AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0932380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0932380 MtrunA17_Chr6g0449311 88.889 144 13 1 37 177 1 144 1.41e-74 222
Msa0932380 MtrunA17_Chr5g0439111 64.557 158 44 4 1 153 1 151 1.85e-60 189
Msa0932380 MtrunA17_Chr6g0452661 59.615 156 51 3 1 153 1 147 9.04e-58 183
Msa0932380 MtrunA17_Chr4g0055441 62.590 139 38 3 10 145 3 130 1.13e-53 169
Msa0932380 MtrunA17_Chr6g0452691 56.081 148 52 3 1 142 1 141 1.41e-52 167
Msa0932380 MtrunA17_Chr6g0452671 55.634 142 56 3 1 140 1 137 6.50e-47 154
Msa0932380 MtrunA17_Chr4g0059901 51.887 106 50 1 8 113 9 113 2.32e-32 118
Msa0932380 MtrunA17_Chr8g0342351 53.333 105 48 1 8 112 11 114 4.32e-31 114
Msa0932380 MtrunA17_Chr3g0097221 46.667 105 55 1 7 111 5 108 2.24e-27 105
Msa0932380 MtrunA17_Chr4g0058651 39.552 134 69 2 7 140 9 130 2.60e-27 103
Msa0932380 MtrunA17_Chr7g0227511 45.918 98 52 1 7 104 9 105 1.49e-26 100
Msa0932380 MtrunA17_Chr2g0330201 46.226 106 56 1 8 113 18 122 7.97e-26 101
Msa0932380 MtrunA17_Chr5g0402611 43.810 105 58 1 7 111 6 109 1.07e-25 101
Msa0932380 MtrunA17_Chr8g0353981 49.398 83 41 1 7 89 5 86 2.05e-25 99.0
Msa0932380 MtrunA17_Chr3g0115431 44.118 102 56 1 10 111 10 110 2.33e-25 99.0
Msa0932380 MtrunA17_Chr3g0117121 44.898 98 53 1 7 104 8 104 6.23e-25 97.1
Msa0932380 MtrunA17_Chr4g0043421 38.931 131 78 2 10 140 16 144 8.26e-25 96.3
Msa0932380 MtrunA17_Chr6g0449641 42.857 98 55 1 7 104 7 103 1.32e-23 92.0
Msa0932380 MtrunA17_Chr5g0437571 38.793 116 69 2 7 122 4 117 2.10e-23 92.4
Msa0932380 MtrunA17_Chr2g0311601 42.553 94 53 1 10 103 14 106 6.41e-23 90.9
Msa0932380 MtrunA17_Chr5g0444351 31.250 144 95 2 10 153 6 145 8.58e-23 90.1
Msa0932380 MtrunA17_Chr6g0449651 41.837 98 56 1 7 104 65 161 1.58e-22 90.9
Msa0932380 MtrunA17_Chr5g0437401 36.697 109 68 1 5 113 7 114 3.33e-22 89.4
Msa0932380 MtrunA17_Chr4g0030001 41.228 114 64 3 10 122 25 136 5.39e-22 89.4
Msa0932380 MtrunA17_Chr3g0106101 37.838 111 67 2 10 120 8 116 5.42e-22 89.0
Msa0932380 MtrunA17_Chr5g0438091 32.353 136 85 2 10 139 40 174 1.33e-21 88.2
Msa0932380 MtrunA17_Chr7g0225111 37.273 110 68 1 10 119 6 114 1.68e-21 85.9
Msa0932380 MtrunA17_Chr8g0374591 39.423 104 62 1 8 111 7 109 1.82e-21 86.3
Msa0932380 MtrunA17_Chr4g0040831 33.333 129 77 3 8 132 14 137 6.80e-21 86.7
Msa0932380 MtrunA17_Chr8g0374601 40.385 104 61 1 8 111 25 127 7.35e-21 88.2
Msa0932380 MtrunA17_Chr1g0200201 37.405 131 72 3 10 132 73 201 1.08e-20 86.7
Msa0932380 MtrunA17_Chr3g0114061 40.000 105 61 2 10 114 9 111 1.74e-20 84.3
Msa0932380 MtrunA17_Chr3g0113891 38.462 104 63 1 5 108 10 112 3.24e-20 84.3
Msa0932380 MtrunA17_Chr3g0129071 37.755 98 60 1 10 107 48 144 9.16e-20 84.3
Msa0932380 MtrunA17_Chr5g0432451 32.143 140 83 2 10 149 44 171 1.46e-19 83.6
Msa0932380 MtrunA17_Chr7g0260971 39.806 103 60 2 10 111 15 116 4.77e-18 79.3
Msa0932380 MtrunA17_Chr8g0351721 36.111 108 67 2 5 111 14 120 1.09e-16 75.9
Msa0932380 MtrunA17_Chr1g0184281 35.652 115 70 3 10 121 16 129 4.93e-16 73.6
Msa0932380 MtrunA17_Chr7g0245871 36.364 99 61 2 10 107 28 125 7.09e-16 73.9
Msa0932380 MtrunA17_Chr8g0351701 34.951 103 65 2 10 111 24 125 9.21e-16 73.6
Msa0932380 MtrunA17_Chr4g0046201 38.636 88 53 1 9 96 6 92 9.52e-16 73.9
Msa0932380 MtrunA17_Chr6g0460951 34.314 102 65 2 10 110 41 141 2.36e-15 72.4
Msa0932380 MtrunA17_Chr8g0374581 35.577 104 65 2 8 110 7 109 2.00e-14 69.7
Msa0932380 MtrunA17_Chr1g0184271 28.082 146 92 4 10 154 13 146 2.89e-14 69.3
Msa0932380 MtrunA17_Chr6g0460941 36.364 88 54 2 10 96 8 94 5.35e-14 65.5
Msa0932380 MtrunA17_Chr5g0435641 29.245 106 73 2 7 111 5 109 1.28e-13 67.0
Msa0932380 MtrunA17_Chr7g0261031 33.000 100 65 2 10 108 9 107 1.99e-13 67.0
Msa0932380 MtrunA17_Chr2g0326041 52.083 48 23 0 10 57 34 81 6.85e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0932380 AT3G47870.1 56.731 104 44 1 9 112 36 138 4.99e-36 129
Msa0932380 AT3G13850.1 43.919 148 78 3 8 152 35 180 1.97e-31 116
Msa0932380 AT3G27650.1 43.000 100 56 1 7 106 37 135 6.42e-25 96.3
Msa0932380 AT5G63090.4 42.727 110 62 1 7 116 9 117 1.16e-24 96.3
Msa0932380 AT5G63090.3 42.727 110 62 1 7 116 9 117 1.16e-24 96.3
Msa0932380 AT5G63090.2 42.727 110 62 1 7 116 9 117 1.16e-24 96.3
Msa0932380 AT5G63090.1 42.727 110 62 1 7 116 9 117 1.16e-24 96.3
Msa0932380 AT1G65620.1 48.193 83 42 1 7 89 7 88 5.24e-24 95.1
Msa0932380 AT1G65620.4 48.193 83 42 1 7 89 7 88 5.24e-24 95.1
Msa0932380 AT1G65620.3 48.193 83 42 1 7 89 7 88 5.24e-24 95.1
Msa0932380 AT1G65620.5 48.193 83 42 1 7 89 7 88 5.24e-24 95.1
Msa0932380 AT1G65620.2 48.193 83 42 1 7 89 7 88 5.24e-24 95.1
Msa0932380 AT5G66870.1 42.157 102 58 1 10 111 8 108 1.12e-23 96.7
Msa0932380 AT3G26660.1 39.130 115 67 2 8 120 4 117 4.13e-23 90.5
Msa0932380 AT1G31320.1 46.316 95 47 2 5 96 6 99 8.98e-23 91.3
Msa0932380 AT3G26620.1 38.095 105 64 1 8 112 4 107 5.33e-22 87.4
Msa0932380 AT1G07900.1 40.000 115 67 2 10 124 34 146 5.43e-22 89.7
Msa0932380 AT1G72980.1 41.964 112 63 2 9 119 13 123 7.51e-22 89.7
Msa0932380 AT2G30130.1 39.474 114 67 2 10 123 9 120 7.97e-21 86.7
Msa0932380 AT2G28500.1 38.261 115 69 2 10 124 56 168 2.49e-20 86.3
Msa0932380 AT2G30340.2 39.796 98 58 1 10 107 54 150 6.17e-20 85.9
Msa0932380 AT2G30340.1 39.796 98 58 1 10 107 53 149 6.88e-20 85.5
Msa0932380 AT2G23660.2 41.584 101 58 1 10 110 6 105 6.89e-20 86.3
Msa0932380 AT2G23660.3 41.584 101 58 1 10 110 6 105 6.89e-20 86.3
Msa0932380 AT2G23660.1 41.584 101 58 1 10 110 6 105 6.89e-20 86.3
Msa0932380 AT3G11090.1 29.518 166 103 3 5 170 7 158 1.66e-19 82.4
Msa0932380 AT2G40470.2 38.776 98 59 1 10 107 21 117 1.97e-19 83.2
Msa0932380 AT1G16530.1 36.752 117 62 4 4 109 6 121 3.29e-19 81.6
Msa0932380 AT2G40470.1 38.776 98 59 1 10 107 46 142 5.03e-19 82.4
Msa0932380 AT5G15060.1 47.297 74 38 1 10 82 18 91 7.36e-19 80.1
Msa0932380 AT1G06280.1 39.362 94 52 3 8 101 23 111 1.98e-17 77.8
Msa0932380 AT3G50510.1 37.383 107 65 2 10 115 13 118 2.07e-17 77.8
Msa0932380 AT3G50510.2 37.383 107 65 2 10 115 13 118 2.07e-17 77.8
Msa0932380 AT2G19820.1 35.354 99 61 1 1 96 1 99 4.03e-17 75.1
Msa0932380 AT2G42430.1 29.655 145 100 2 10 153 16 159 8.04e-17 77.0
Msa0932380 AT4G00210.1 34.058 138 83 4 10 145 12 143 2.93e-16 75.1
Msa0932380 AT2G42440.1 32.407 108 71 2 10 116 8 114 2.27e-15 73.2
Msa0932380 AT4G00220.1 35.644 101 63 2 12 111 20 119 2.87e-15 72.4
Msa0932380 AT3G58190.1 34.579 107 68 2 10 115 12 117 3.33e-15 72.0
Msa0932380 AT2G45420.1 34.653 101 64 2 12 111 40 139 1.71e-14 70.9
Msa0932380 AT3G03760.1 35.294 102 64 2 10 110 52 152 4.12e-14 70.1
Msa0932380 AT5G35900.1 29.412 102 71 1 10 111 6 106 1.73e-13 67.0
Msa0932380 AT2G45410.1 36.634 101 62 2 12 111 19 118 3.00e-13 66.2
Msa0932380 AT2G31310.1 29.508 122 76 3 10 122 8 128 5.87e-13 65.5
Msa0932380 AT5G06080.1 31.959 97 64 2 9 104 7 102 1.50e-12 63.9

Find 27 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCAACATTGCTAGATAT+TGG 0.233878 6_3:+452260 Msa0932370:intron
TCACCAACATTGTTAAATAT+TGG 0.234385 6_3:+452464 Msa0932370:intron
CTTTCTTCTTTGATATTTGC+AGG 0.243555 6_3:+452847 Msa0932370:intron
ATTATTATGTGATAATAATA+AGG 0.272056 6_3:+452437 Msa0932370:intron
TGGATGTCTTGGTCTTATTA+TGG 0.281895 6_3:-452633 Msa0932380:CDS
GGTTTATCGGCTGGAAAATA+AGG 0.296290 6_3:+452800 Msa0932370:intron
ATAATGAATACTCAAGTTCT+TGG 0.299220 6_3:-452419 Msa0932380:CDS
GCCAATGCTCATCGTTTGTT+TGG 0.347008 6_3:-452767 Msa0932380:CDS
TAAGGAGCTAAAGGGCAATC+TGG 0.416523 6_3:+452818 Msa0932370:intron
ACCACTAGTTCTCAAATTCC+AGG 0.434386 6_3:-452290 Msa0932380:CDS
ATTCCAATATTTAACAATGT+TGG 0.448330 6_3:-452467 Msa0932380:CDS
TCTTCTACATTTCTCTTCAT+CGG 0.464845 6_3:-452522 Msa0932380:CDS
GGAATTGAAGAACTAGTAGA+AGG 0.471090 6_3:+452485 Msa0932370:intron
TTCCCAAGTCATGGATGTCT+TGG 0.482571 6_3:-452644 Msa0932380:CDS
GCTGGAAAATAAGGAGCTAA+AGG 0.488184 6_3:+452809 Msa0932370:intron
CTAGATATTGGATTAATACC+TGG 0.493908 6_3:+452272 Msa0932370:intron
CTGGAAAATAAGGAGCTAAA+GGG 0.495690 6_3:+452810 Msa0932370:intron
GAATGATCCATTGAAAAGTA+AGG 0.496518 6_3:+452323 Msa0932370:intron
AATCCAATATCTAGCAATGT+TGG 0.514330 6_3:-452263 Msa0932380:CDS
AGACCAAGACATCCATGACT+TGG 0.541471 6_3:+452641 Msa0932370:intron
ACCAAACAAACGATGAGCAT+TGG 0.553402 6_3:+452766 Msa0932370:intron
CGTGCAAGATTCCCAAGTCA+TGG 0.565788 6_3:-452653 Msa0932380:CDS
GTAAATTTGTTAACATCGTG+AGG 0.570877 6_3:+452229 Msa0932370:intron
ACCTGGAATTTGAGAACTAG+TGG 0.587717 6_3:+452289 Msa0932370:intron
GACCAAGACATCCATGACTT+GGG 0.599785 6_3:+452642 Msa0932370:intron
TTTGTTTGGTATTTCCAACA+TGG 0.612102 6_3:-452753 Msa0932380:CDS
GGAATTTGAGAACTAGTGGT+AGG 0.664884 6_3:+452293 Msa0932370:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATGTGATAATAATA+AGG + chr6_3:452685-452704 Msa0932370:intron 10.0%
!! ATTCCAATATTTAACAATGT+TGG - chr6_3:452652-452671 Msa0932380:CDS 20.0%
!!! GAAGTCAATTATTTTTGAGT+CGG - chr6_3:452438-452457 Msa0932380:CDS 25.0%
! TCACCAACATTGTTAAATAT+TGG + chr6_3:452658-452677 Msa0932370:intron 25.0%
! ATAATGAATACTCAAGTTCT+TGG - chr6_3:452700-452719 Msa0932380:CDS 25.0%
!!! TTTTCTACCTTACTTTTCAA+TGG - chr6_3:452789-452808 Msa0932380:CDS 25.0%
GGATATTCACAGAAAAAACA+AGG - chr6_3:452240-452259 Msa0932380:CDS 30.0%
CTTTCTTCTTTGATATTTGC+AGG + chr6_3:452275-452294 Msa0932370:intron 30.0%
TTTGTTTGGTATTTCCAACA+TGG - chr6_3:452366-452385 Msa0932380:CDS 30.0%
TCTTCTACATTTCTCTTCAT+CGG - chr6_3:452597-452616 Msa0932380:CDS 30.0%
GAATGATCCATTGAAAAGTA+AGG + chr6_3:452799-452818 Msa0932370:intron 30.0%
CTAGATATTGGATTAATACC+TGG + chr6_3:452850-452869 Msa0932370:intron 30.0%
AATCCAATATCTAGCAATGT+TGG - chr6_3:452856-452875 Msa0932380:CDS 30.0%
TTACCAACATTGCTAGATAT+TGG + chr6_3:452862-452881 Msa0932370:intron 30.0%
CTGGAAAATAAGGAGCTAAA+GGG + chr6_3:452312-452331 Msa0932370:intron 35.0%
! TTTTAGCATCTTCACCATGT+TGG + chr6_3:452383-452402 Msa0932370:intron 35.0%
!! TGGATGTCTTGGTCTTATTA+TGG - chr6_3:452486-452505 Msa0932380:CDS 35.0%
GGAATTGAAGAACTAGTAGA+AGG + chr6_3:452637-452656 Msa0932370:intron 35.0%
GCTGGAAAATAAGGAGCTAA+AGG + chr6_3:452313-452332 Msa0932370:intron 40.0%
! GGTTTATCGGCTGGAAAATA+AGG + chr6_3:452322-452341 Msa0932370:intron 40.0%
!!! AGCATTTTTGGTTTATCGGC+TGG + chr6_3:452331-452350 Msa0932370:intron 40.0%
!!! GGCGAGCATTTTTGGTTTAT+CGG + chr6_3:452335-452354 Msa0932370:intron 40.0%
ACCAAACAAACGATGAGCAT+TGG + chr6_3:452356-452375 Msa0932370:intron 40.0%
GGAATTTGAGAACTAGTGGT+AGG + chr6_3:452829-452848 Msa0932370:intron 40.0%
! ACCACTAGTTCTCAAATTCC+AGG - chr6_3:452829-452848 Msa0932380:CDS 40.0%
ACCTGGAATTTGAGAACTAG+TGG + chr6_3:452833-452852 Msa0932370:intron 40.0%
TAAGGAGCTAAAGGGCAATC+TGG + chr6_3:452304-452323 Msa0932370:intron 45.0%
!!! TGAGCATTGGCGAGCATTTT+TGG + chr6_3:452343-452362 Msa0932370:intron 45.0%
GCCAATGCTCATCGTTTGTT+TGG - chr6_3:452352-452371 Msa0932380:CDS 45.0%
TTCCCAAGTCATGGATGTCT+TGG - chr6_3:452475-452494 Msa0932380:CDS 45.0%
GACCAAGACATCCATGACTT+GGG + chr6_3:452480-452499 Msa0932370:intron 45.0%
AGACCAAGACATCCATGACT+TGG + chr6_3:452481-452500 Msa0932370:intron 45.0%
CGTGCAAGATTCCCAAGTCA+TGG - chr6_3:452466-452485 Msa0932380:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 452238 452903 452238 ID=Msa0932380;Name=Msa0932380
chr6_3 mRNA 452238 452903 452238 ID=Msa0932380-mRNA-1;Parent=Msa0932380;Name=Msa0932380-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|-1|0|1|-1|1|1|0|221
chr6_3 exon 452238 452903 452238 ID=Msa0932380-mRNA-1:exon:187;Parent=Msa0932380-mRNA-1
chr6_3 CDS 452238 452903 452238 ID=Msa0932380-mRNA-1:cds;Parent=Msa0932380-mRNA-1
Gene Sequence

>Msa0932380

ATGGATATTCACAGAAAAAACAAGGCATGTGCAGCCTGCAAATATCAAAGAAGAAAGTGTACTCCAGATTGCCCTTTAGCTCCTTATTTTCCAGCCGATAAACCAAAAATGCTCGCCAATGCTCATCGTTTGTTTGGTATTTCCAACATGGTGAAGATGCTAAAATCTATTGACGATGATGATCATAAAGATGAAGCAATGAAGTCAATTATTTTTGAGTCGGAAATGCGTGCAAGATTCCCAAGTCATGGATGTCTTGGTCTTATTATGGAGTATCAAGATATGATAATTGAGTCTATGAAAGAGTTAGATCATGTAAAACAGTTACTAAGTTTGTGTATGCTATCACAACAACAAAATCTTCTACATTTCTCTTCATCGGATCATTCTTCAGTACCTTCTACTAGTTCTTCAATTCCAATATTTAACAATGTTGGTGATGTTCCTTATTATTATCACATAATAATGAATACTCAAGTTCTTGGAAATCTTGATATGATAAAGTATGAAGAAGAGTTAGATTATGTAAAAAATAATCTACAGCAGCAAAATTTTCTACCTTACTTTTCAATGGATCATTCTTCTGTACCTACCACTAGTTCTCAAATTCCAGGTATTAATCCAATATCTAGCAATGTTGGTAACATTCATTCCTCACGATGTTAA

Protein sequence

>Msa0932380

MDIHRKNKACAACKYQRRKCTPDCPLAPYFPADKPKMLANAHRLFGISNMVKMLKSIDDDDHKDEAMKSIIFESEMRARFPSHGCLGLIMEYQDMIIESMKELDHVKQLLSLCMLSQQQNLLHFSSSDHSSVPSTSSSIPIFNNVGDVPYYYHIIMNTQVLGNLDMIKYEEELDYVKNNLQQQNFLPYFSMDHSSVPTTSSQIPGINPISSNVGNIHSSRC*