AlfalfaGEDB | Msa0936850

M. sativa cultivar zhongmu-4 / Msa0936850

KEGG: K14400
GO: GO:0000993 GO:0001098 GO:0001099 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005849 GO:0006139 GO:0006351 GO:0006353 GO:0006366 GO:0006369 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009909 GO:0009911 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2000026 GO:2000241 GO:2000243
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936850	sp\|Q0WPF2\|PCFS4_ARATH	69.347	199	61	0	234	432	591	789	1.03e-96	310

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936850	A0A2K3PQM1	64.118	510	89	12	15	433	250	756	0.0	603

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TFs/TRs:

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Gene ID	Type	Classification
Msa0936850	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936850	MtrunA17_Chr6g0452881	65.497	513	80	10	15	433	304	813	0.0	596
Msa0936850	MtrunA17_Chr6g0452891	81.513	238	22	3	197	433	1	217	2.40e-135	387
Msa0936850	MtrunA17_Chr4g0037071	82.514	183	32	0	251	433	1	183	4.49e-100	298
Msa0936850	MtrunA17_Chr4g0037081	54.491	167	32	5	6	130	15	179	3.44e-43	150
Msa0936850	MtrunA17_Chr1g0179281	36.813	182	109	4	256	433	829	1008	2.57e-35	139
Msa0936850	MtrunA17_Chr7g0265781	34.314	204	127	5	234	433	815	1015	4.52e-34	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936850	AT4G04885.1	69.347	199	61	0	234	432	591	789	1.05e-97	310
Msa0936850	AT1G66500.1	35.503	338	136	15	159	431	66	386	1.26e-46	166
Msa0936850	AT5G43620.1	34.957	349	149	16	131	431	62	380	1.88e-46	165
Msa0936850	AT2G36480.2	33.533	167	102	3	265	431	785	942	2.19e-25	109
Msa0936850	AT2G36480.1	33.533	167	102	3	265	431	785	942	2.19e-25	109

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Find 119 sgRNAs with CRISPR-Local

Find 340 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGATGAAGAATTTGT+TGG	0.474608	6_3:-7776163	None:intergenic
AAAGAAAGAAGATGAAGAGT+TGG	0.403383	6_3:+7777421	Msa0936850:CDS
AAAGGAAGAAGATGCTTGCC+AGG	0.480340	6_3:+7776131	None:intergenic
AAAGTTGCTGGCGAAACTGG+TGG	0.614136	6_3:-7776451	None:intergenic
AAATTAGAATATCCGACAGT+TGG	0.551878	6_3:-7776882	None:intergenic
AAGAGTTGTTTCCGATTCTT+TGG	0.325210	6_3:-7776776	None:intergenic
AATACTACCACTGTGAGTCA+TGG	0.609522	6_3:+7776568	Msa0936850:CDS
AATATCCGACAGTTGGCTGT+TGG	0.554695	6_3:-7776875	None:intergenic
AATTAGACCATGACTCACAG+TGG	0.751691	6_3:-7776575	None:intergenic
ACAGTGATGAAACGGAGGAG+TGG	0.635697	6_3:+7777507	Msa0936850:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTAGAATATCCGACAGT+TGG	-	chr6_3:7776885-7776904	None:intergenic	30.0%
GGTACGCAATTAAACAATAA+TGG	-	chr6_3:7777078-7777097	None:intergenic	30.0%
GAATATTTGGATCAAACTCA+AGG	-	chr6_3:7777139-7777158	None:intergenic	30.0%
GTTTGATCCAAATATTCTCA+AGG	+	chr6_3:7777142-7777161	Msa0936850:CDS	30.0%
AAAGAAAGAAGATGAAGAGT+TGG	+	chr6_3:7777421-7777440	Msa0936850:CDS	30.0%
CAAAATACATGTGCATTATG+TGG	+	chr6_3:7777464-7777483	Msa0936850:CDS	30.0%
TCTGCATTTAGCATGAATAA+CGG	-	chr6_3:7777606-7777625	None:intergenic	30.0%
TTCATCACAAAATCTTCAGA+CGG	-	chr6_3:7777641-7777660	None:intergenic	30.0%
AAGTCACCGAAAGAATAATA+TGG	-	chr6_3:7777713-7777732	None:intergenic	30.0%
AAATTAGAATATCCGACAGT+TGG	-	chr6_3:7776885-7776904	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	7776135	7778090	7776135	ID=Msa0936850;Name=Msa0936850
chr6_3	mRNA	7776135	7778090	7776135	ID=Msa0936850-mRNA-1;Parent=Msa0936850;Name=Msa0936850-mRNA-1;_AED=0.24;_eAED=0.24;_QI=7\|0.66\|0.5\|1\|0\|0\|4\|75\|433
chr6_3	exon	7776135	7776707	7776135	ID=Msa0936850-mRNA-1:exon:2556;Parent=Msa0936850-mRNA-1
chr6_3	exon	7776734	7776962	7776734	ID=Msa0936850-mRNA-1:exon:2557;Parent=Msa0936850-mRNA-1
chr6_3	exon	7777125	7777667	7777125	ID=Msa0936850-mRNA-1:exon:2558;Parent=Msa0936850-mRNA-1
chr6_3	exon	7778052	7778090	7778052	ID=Msa0936850-mRNA-1:exon:2559;Parent=Msa0936850-mRNA-1
chr6_3	five_prime_UTR	7776135	7776141	7776135	ID=Msa0936850-mRNA-1:five_prime_utr;Parent=Msa0936850-mRNA-1
chr6_3	CDS	7776142	7776707	7776142	ID=Msa0936850-mRNA-1:cds;Parent=Msa0936850-mRNA-1
chr6_3	CDS	7776734	7776962	7776734	ID=Msa0936850-mRNA-1:cds;Parent=Msa0936850-mRNA-1
chr6_3	CDS	7777125	7777631	7777125	ID=Msa0936850-mRNA-1:cds;Parent=Msa0936850-mRNA-1
chr6_3	three_prime_UTR	7777632	7777667	7777632	ID=Msa0936850-mRNA-1:three_prime_utr;Parent=Msa0936850-mRNA-1
chr6_3	three_prime_UTR	7778052	7778090	7778052	ID=Msa0936850-mRNA-1:three_prime_utr;Parent=Msa0936850-mRNA-1

Gene Sequence

>Msa0936850

ATGCTTGCCAGGAGAGAAACACCAACAAATTCTTCATCAGTTTTCTCACACTTTCCGCGAAAAAGATGTTTTGATGAGTTCAGCTATCGCAGCAACGGACAACCTCGTCAAAATCCAATAGCTCTAATTGATGCCTATGGTAGTGCGAAAAGACAAGAAACTTCATGGAAGAATACCGAAGAGGAGGAGTTCAATTGGGAAGATATGATTCCGACACCAGCAACTGGGAAGCCCGCTACTATTGCAGCAAATGCAACTTTTTCAGCGTCAAGGGTGTTTCCTGGTCTCAATTCTAATACTGAGTACCATCCACCAGTTTCGCCAGCAACTTTTGAAACGAGGCCCTCCGTAAATGTGCATGCTCCACGTCCTCCTATATATCCATCGAAGAATCCTGTTAGGAATCCATTCGAATCAATAAATGCTAATACTACCACTGTGAGTCATGGTCTAATTAATAGACCTATTCCTGTGCATGAACATGGTGTCGAGAACAATGGTATCAGTAAAAGAAACCTGCATCAGTTGCCTAACTCGCCAACCAGTGGACAAACACCGCCTTTCCACGTCGCAAATGCACGACCTGTTATGCCACTTCCTATGCCAGACCAAAGAATCGGAAACAACTCTTTCCGACCTTTACTTCCAGCTGGCCATGCTCTACCCCAGATTCTACCTCATCCGAATCCTGGTCCTAATGTGTCAAGCCAACAGCCAACTGTCGGATATTCTAATTTGATTAGTTCACTCGTGGCCCAAGGTGTTATCTCATTAGCTAATCAAGCCCCTGCGGAGGATTTTATTGGCCTTGAGTTTGATCCAAATATTCTCAAGGTTCGTCATAAATCTGTAATCCGTGGCTTATATGGTGATTTTCCTAGACAGTGCACAACTTGTGGCCTCCGATTCAAATCCAAAGATGAGCACAGCAGTCACATGGATTGGCATGTAACTAAGAATCGTATGTCTAAAAACAATAAGCAGAAACCCTCTCGCAAGTGGTTTGTGAGTGAGACGATGTGGCTTAAAGGTGCCGAGGCATTGGGAGTCAAGTCAGCTCCAGGTTTTTTGCCAACCGAGACCACTGAAGAAAAGAAAGAAGATGAAGAGTTGGCTGTTCTGGCCGACGAGGACCAAAATACATGTGCATTATGTGGAGAACCTTTTGAGGAGTTTTACAGTGATGAAACGGAGGAGTGGATGTATAGAGGAGCTGTATACCTTAATGCGCCTAATGGAATAACAACAGGCATAGACAGGTCGCAGTTAGGCCCCGTTATTCATGCTAAATGCAGATCTTAA

Protein sequence

>Msa0936850

MLARRETPTNSSSVFSHFPRKRCFDEFSYRSNGQPRQNPIALIDAYGSAKRQETSWKNTEEEEFNWEDMIPTPATGKPATIAANATFSASRVFPGLNSNTEYHPPVSPATFETRPSVNVHAPRPPIYPSKNPVRNPFESINANTTTVSHGLINRPIPVHEHGVENNGISKRNLHQLPNSPTSGQTPPFHVANARPVMPLPMPDQRIGNNSFRPLLPAGHALPQILPHPNPGPNVSSQQPTVGYSNLISSLVAQGVISLANQAPAEDFIGLEFDPNILKVRHKSVIRGLYGDFPRQCTTCGLRFKSKDEHSSHMDWHVTKNRMSKNNKQKPSRKWFVSETMWLKGAEALGVKSAPGFLPTETTEEKKEDEELAVLADEDQNTCALCGEPFEEFYSDETEEWMYRGAVYLNAPNGITTGIDRSQLGPVIHAKCRS*