Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0937090 | A0A072U878 | 63.359 | 131 | 10 | 2 | 1 | 93 | 1 | 131 | 1.72e-46 | 155 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0937090 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0937090 | MtrunA17_Chr3g0082121 | 70.769 | 65 | 19 | 0 | 29 | 93 | 52 | 116 | 2.49e-27 | 103 |
| Msa0937090 | MtrunA17_Chr5g0401121 | 63.077 | 65 | 24 | 0 | 29 | 93 | 65 | 129 | 9.94e-22 | 83.2 |
| Msa0937090 | MtrunA17_Chr6g0458391 | 46.154 | 65 | 35 | 0 | 29 | 93 | 234 | 298 | 3.78e-17 | 74.7 |
| Msa0937090 | MtrunA17_Chr6g0458391 | 46.774 | 62 | 32 | 1 | 32 | 93 | 103 | 163 | 2.58e-11 | 58.2 |
| Msa0937090 | MtrunA17_Chr1g0203721 | 46.154 | 65 | 35 | 0 | 29 | 93 | 72 | 136 | 4.28e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGTATTCGTCAAGAATA+TGG | 0.248739 | 6_3:-8169117 | Msa0937090:CDS |
| TCTGCTCTAGGCTCCCCTTT+TGG | 0.322934 | 6_3:+8169006 | None:intergenic |
| AATGAGTATAGTAAAAGAAT+TGG | 0.336174 | 6_3:-8169141 | Msa0937090:CDS |
| GGAAATAAAAGCAGAGTTGA+TGG | 0.361988 | 6_3:-8169096 | Msa0937090:CDS |
| TATAGTAAAAGAATTGGATT+TGG | 0.393932 | 6_3:-8169135 | Msa0937090:CDS |
| CCTGTTCTAATTTCTGCTCT+AGG | 0.415588 | 6_3:+8168994 | None:intergenic |
| AACAACAATGGCAGCGGCGC+AGG | 0.452252 | 6_3:+8169748 | None:intergenic |
| GGATGGTCGGAGACGGCGGC+GGG | 0.454411 | 6_3:+8169770 | None:intergenic |
| GACAAAAGACGTCAACCAAA+AGG | 0.461065 | 6_3:-8169021 | Msa0937090:CDS |
| GGGATGGTCGGAGACGGCGG+CGG | 0.462743 | 6_3:+8169769 | None:intergenic |
| TAGTGTTGGTAGCAGGATGA+TGG | 0.465525 | 6_3:+8169798 | None:intergenic |
| GGTTCGTTAGTGTTGGTAGC+AGG | 0.468658 | 6_3:+8169791 | None:intergenic |
| GCTGCCATTGTTGTTGATCA+AGG | 0.480338 | 6_3:-8169740 | Msa0937090:intron |
| AGTGTTGGTAGCAGGATGAT+GGG | 0.490017 | 6_3:+8169799 | None:intergenic |
| ACAAAAGACGTCAACCAAAA+GGG | 0.496414 | 6_3:-8169020 | Msa0937090:CDS |
| CCTAGAGCAGAAATTAGAAC+AGG | 0.510209 | 6_3:-8168994 | Msa0937090:CDS |
| GGCAGCGGCGCAGGGATGGT+CGG | 0.512257 | 6_3:+8169757 | None:intergenic |
| CAATGGCAGCGGCGCAGGGA+TGG | 0.526019 | 6_3:+8169753 | None:intergenic |
| GGCGCAGGGATGGTCGGAGA+CGG | 0.533543 | 6_3:+8169763 | None:intergenic |
| GCAGGGATGGTCGGAGACGG+CGG | 0.559162 | 6_3:+8169766 | None:intergenic |
| ACAACAATGGCAGCGGCGCA+GGG | 0.575609 | 6_3:+8169749 | None:intergenic |
| GGCGGCGGGTTCGTTAGTGT+TGG | 0.578521 | 6_3:+8169784 | None:intergenic |
| GTGTTGGTAGCAGGATGATG+GGG | 0.582430 | 6_3:+8169800 | None:intergenic |
| CAAAAGACGTCAACCAAAAG+GGG | 0.607006 | 6_3:-8169019 | Msa0937090:CDS |
| AAGATTCACATGCTACAAAG+AGG | 0.619398 | 6_3:-8169058 | Msa0937090:CDS |
| TTGATCAACAACAATGGCAG+CGG | 0.627743 | 6_3:+8169742 | None:intergenic |
| TGCTACAAAGAGGGTGTACG+AGG | 0.638548 | 6_3:-8169048 | Msa0937090:CDS |
| AGATTCACATGCTACAAAGA+GGG | 0.654496 | 6_3:-8169057 | Msa0937090:CDS |
| ATTACCTTGATCAACAACAA+TGG | 0.673823 | 6_3:+8169736 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTACTATTAAAAAAACATT+GGG | + | chr6_3:8169272-8169291 | None:intergenic | 10.0% |
| !! | TATTACTATTAAAAAAACAT+TGG | + | chr6_3:8169273-8169292 | None:intergenic | 10.0% |
| !! | ATTACTATTAAAAAAACATT+GGG | + | chr6_3:8169272-8169291 | None:intergenic | 10.0% |
| !! | TATTACTATTAAAAAAACAT+TGG | + | chr6_3:8169273-8169292 | None:intergenic | 10.0% |
| !!! | TATACATATATATAGCTTTA+TGG | - | chr6_3:8169207-8169226 | Msa0937090:intron | 15.0% |
| !! | TTACTATTAAAAAAACATTG+GGG | + | chr6_3:8169271-8169290 | None:intergenic | 15.0% |
| !! | AAAACCAAAATTTACAATTA+AGG | + | chr6_3:8169508-8169527 | None:intergenic | 15.0% |
| !!! | TATACATATATATAGCTTTA+TGG | - | chr6_3:8169207-8169226 | Msa0937090:intron | 15.0% |
| !! | TTACTATTAAAAAAACATTG+GGG | + | chr6_3:8169271-8169290 | None:intergenic | 15.0% |
| !! | AAAACCAAAATTTACAATTA+AGG | + | chr6_3:8169508-8169527 | None:intergenic | 15.0% |
| !! | GACAAATAATAAAATAGAGA+AGG | + | chr6_3:8169100-8169119 | None:intergenic | 20.0% |
| !!! | TTGATGAAAAATTAGTTTCT+AGG | - | chr6_3:8169152-8169171 | Msa0937090:CDS | 20.0% |
| !! | TACTATTAAAAAAACATTGG+GGG | + | chr6_3:8169270-8169289 | None:intergenic | 20.0% |
| !!! | AATAGTAATAGATAGCTTTA+TGG | - | chr6_3:8169283-8169302 | Msa0937090:intron | 20.0% |
| !! | AATGAGTATAGTAAAAGAAT+TGG | - | chr6_3:8169661-8169680 | Msa0937090:intron | 20.0% |
| !! | TATAGTAAAAGAATTGGATT+TGG | - | chr6_3:8169667-8169686 | Msa0937090:intron | 20.0% |
| !! | GACAAATAATAAAATAGAGA+AGG | + | chr6_3:8169100-8169119 | None:intergenic | 20.0% |
| !!! | TTGATGAAAAATTAGTTTCT+AGG | - | chr6_3:8169152-8169171 | Msa0937090:CDS | 20.0% |
| !! | TACTATTAAAAAAACATTGG+GGG | + | chr6_3:8169270-8169289 | None:intergenic | 20.0% |
| !!! | AATAGTAATAGATAGCTTTA+TGG | - | chr6_3:8169283-8169302 | Msa0937090:intron | 20.0% |
| !! | AATGAGTATAGTAAAAGAAT+TGG | - | chr6_3:8169661-8169680 | Msa0937090:intron | 20.0% |
| !! | TATAGTAAAAGAATTGGATT+TGG | - | chr6_3:8169667-8169686 | Msa0937090:intron | 20.0% |
| ! | TTCAGATTGTGTCAATAAAT+TGG | - | chr6_3:8169528-8169547 | Msa0937090:intron | 25.0% |
| ! | TTCAGATTGTGTCAATAAAT+TGG | - | chr6_3:8169528-8169547 | Msa0937090:intron | 25.0% |
| ATTACCTTGATCAACAACAA+TGG | + | chr6_3:8169069-8169088 | None:intergenic | 30.0% | |
| TTAAAAAAACATTGGGGGAA+AGG | + | chr6_3:8169265-8169284 | None:intergenic | 30.0% | |
| !! | GTGGCCTTAATTGTAAATTT+TGG | - | chr6_3:8169501-8169520 | Msa0937090:intron | 30.0% |
| GGAAATGAAGTTTGATTATG+AGG | - | chr6_3:8169618-8169637 | Msa0937090:intron | 30.0% | |
| TTTGGTATTCGTCAAGAATA+TGG | - | chr6_3:8169685-8169704 | Msa0937090:intron | 30.0% | |
| ATTACCTTGATCAACAACAA+TGG | + | chr6_3:8169069-8169088 | None:intergenic | 30.0% | |
| TTAAAAAAACATTGGGGGAA+AGG | + | chr6_3:8169265-8169284 | None:intergenic | 30.0% | |
| !! | GTGGCCTTAATTGTAAATTT+TGG | - | chr6_3:8169501-8169520 | Msa0937090:intron | 30.0% |
| GGAAATGAAGTTTGATTATG+AGG | - | chr6_3:8169618-8169637 | Msa0937090:intron | 30.0% | |
| TTTGGTATTCGTCAAGAATA+TGG | - | chr6_3:8169685-8169704 | Msa0937090:intron | 30.0% | |
| ! | ATGCAGTCTAAGTTTTCTAG+TGG | - | chr6_3:8169319-8169338 | Msa0937090:intron | 35.0% |
| !! | AAAACAACATTCTGAAGTCG+CGG | + | chr6_3:8169427-8169446 | None:intergenic | 35.0% |
| ! | ATGTCTGAAATCGTCACTTA+TGG | - | chr6_3:8169479-8169498 | Msa0937090:intron | 35.0% |
| CATGAACAAAATGTGCAACA+CGG | - | chr6_3:8169562-8169581 | Msa0937090:intron | 35.0% | |
| TCAAACTTCATTTCCAAGTG+AGG | + | chr6_3:8169613-8169632 | None:intergenic | 35.0% | |
| GGAAATAAAAGCAGAGTTGA+TGG | - | chr6_3:8169706-8169725 | Msa0937090:intron | 35.0% | |
| AAGATTCACATGCTACAAAG+AGG | - | chr6_3:8169744-8169763 | Msa0937090:CDS | 35.0% | |
| AGATTCACATGCTACAAAGA+GGG | - | chr6_3:8169745-8169764 | Msa0937090:CDS | 35.0% | |
| ACAAAAGACGTCAACCAAAA+GGG | - | chr6_3:8169782-8169801 | Msa0937090:CDS | 35.0% | |
| ! | ATGCAGTCTAAGTTTTCTAG+TGG | - | chr6_3:8169319-8169338 | Msa0937090:intron | 35.0% |
| !! | AAAACAACATTCTGAAGTCG+CGG | + | chr6_3:8169427-8169446 | None:intergenic | 35.0% |
| ! | ATGTCTGAAATCGTCACTTA+TGG | - | chr6_3:8169479-8169498 | Msa0937090:intron | 35.0% |
| CATGAACAAAATGTGCAACA+CGG | - | chr6_3:8169562-8169581 | Msa0937090:intron | 35.0% | |
| TCAAACTTCATTTCCAAGTG+AGG | + | chr6_3:8169613-8169632 | None:intergenic | 35.0% | |
| GGAAATAAAAGCAGAGTTGA+TGG | - | chr6_3:8169706-8169725 | Msa0937090:intron | 35.0% | |
| AAGATTCACATGCTACAAAG+AGG | - | chr6_3:8169744-8169763 | Msa0937090:CDS | 35.0% | |
| AGATTCACATGCTACAAAGA+GGG | - | chr6_3:8169745-8169764 | Msa0937090:CDS | 35.0% | |
| ACAAAAGACGTCAACCAAAA+GGG | - | chr6_3:8169782-8169801 | Msa0937090:CDS | 35.0% | |
| TTGATCAACAACAATGGCAG+CGG | + | chr6_3:8169063-8169082 | None:intergenic | 40.0% | |
| AAAAACATTGGGGGAAAGGA+TGG | + | chr6_3:8169261-8169280 | None:intergenic | 40.0% | |
| ! | TCTGAAATCGTCACTTATGG+TGG | - | chr6_3:8169482-8169501 | Msa0937090:intron | 40.0% |
| TTCCAAGTGAGGTACATTGT+TGG | + | chr6_3:8169602-8169621 | None:intergenic | 40.0% | |
| GAAGTTTGATTATGAGGCAG+CGG | - | chr6_3:8169624-8169643 | Msa0937090:intron | 40.0% | |
| GACAAAAGACGTCAACCAAA+AGG | - | chr6_3:8169781-8169800 | Msa0937090:CDS | 40.0% | |
| CAAAAGACGTCAACCAAAAG+GGG | - | chr6_3:8169783-8169802 | Msa0937090:CDS | 40.0% | |
| CCTGTTCTAATTTCTGCTCT+AGG | + | chr6_3:8169811-8169830 | None:intergenic | 40.0% | |
| CCTAGAGCAGAAATTAGAAC+AGG | - | chr6_3:8169808-8169827 | Msa0937090:CDS | 40.0% | |
| TTGATCAACAACAATGGCAG+CGG | + | chr6_3:8169063-8169082 | None:intergenic | 40.0% | |
| AAAAACATTGGGGGAAAGGA+TGG | + | chr6_3:8169261-8169280 | None:intergenic | 40.0% | |
| ! | TCTGAAATCGTCACTTATGG+TGG | - | chr6_3:8169482-8169501 | Msa0937090:intron | 40.0% |
| TTCCAAGTGAGGTACATTGT+TGG | + | chr6_3:8169602-8169621 | None:intergenic | 40.0% | |
| GAAGTTTGATTATGAGGCAG+CGG | - | chr6_3:8169624-8169643 | Msa0937090:intron | 40.0% | |
| GACAAAAGACGTCAACCAAA+AGG | - | chr6_3:8169781-8169800 | Msa0937090:CDS | 40.0% | |
| CAAAAGACGTCAACCAAAAG+GGG | - | chr6_3:8169783-8169802 | Msa0937090:CDS | 40.0% | |
| CCTGTTCTAATTTCTGCTCT+AGG | + | chr6_3:8169811-8169830 | None:intergenic | 40.0% | |
| CCTAGAGCAGAAATTAGAAC+AGG | - | chr6_3:8169808-8169827 | Msa0937090:CDS | 40.0% | |
| !! | AGTGTTGGTAGCAGGATGAT+GGG | + | chr6_3:8169006-8169025 | None:intergenic | 45.0% |
| !! | TAGTGTTGGTAGCAGGATGA+TGG | + | chr6_3:8169007-8169026 | None:intergenic | 45.0% |
| ! | GCTGCCATTGTTGTTGATCA+AGG | - | chr6_3:8169062-8169081 | Msa0937090:CDS | 45.0% |
| CAAAATGTGCAACACGGACA+AGG | - | chr6_3:8169568-8169587 | Msa0937090:intron | 45.0% | |
| TGCCAACAATGTACCTCACT+TGG | - | chr6_3:8169597-8169616 | Msa0937090:intron | 45.0% | |
| !! | AGTGTTGGTAGCAGGATGAT+GGG | + | chr6_3:8169006-8169025 | None:intergenic | 45.0% |
| !! | TAGTGTTGGTAGCAGGATGA+TGG | + | chr6_3:8169007-8169026 | None:intergenic | 45.0% |
| ! | GCTGCCATTGTTGTTGATCA+AGG | - | chr6_3:8169062-8169081 | Msa0937090:CDS | 45.0% |
| CAAAATGTGCAACACGGACA+AGG | - | chr6_3:8169568-8169587 | Msa0937090:intron | 45.0% | |
| TGCCAACAATGTACCTCACT+TGG | - | chr6_3:8169597-8169616 | Msa0937090:intron | 45.0% | |
| !! | GTGTTGGTAGCAGGATGATG+GGG | + | chr6_3:8169005-8169024 | None:intergenic | 50.0% |
| !! | GGTTCGTTAGTGTTGGTAGC+AGG | + | chr6_3:8169014-8169033 | None:intergenic | 50.0% |
| ! | TGCTACAAAGAGGGTGTACG+AGG | - | chr6_3:8169754-8169773 | Msa0937090:CDS | 50.0% |
| !! | GTGTTGGTAGCAGGATGATG+GGG | + | chr6_3:8169005-8169024 | None:intergenic | 50.0% |
| !! | GGTTCGTTAGTGTTGGTAGC+AGG | + | chr6_3:8169014-8169033 | None:intergenic | 50.0% |
| ! | TGCTACAAAGAGGGTGTACG+AGG | - | chr6_3:8169754-8169773 | Msa0937090:CDS | 50.0% |
| !! | TCTGCTCTAGGCTCCCCTTT+TGG | + | chr6_3:8169799-8169818 | None:intergenic | 55.0% |
| !! | TCTGCTCTAGGCTCCCCTTT+TGG | + | chr6_3:8169799-8169818 | None:intergenic | 55.0% |
| ACAACAATGGCAGCGGCGCA+GGG | + | chr6_3:8169056-8169075 | None:intergenic | 60.0% | |
| AACAACAATGGCAGCGGCGC+AGG | + | chr6_3:8169057-8169076 | None:intergenic | 60.0% | |
| ACAACAATGGCAGCGGCGCA+GGG | + | chr6_3:8169056-8169075 | None:intergenic | 60.0% | |
| AACAACAATGGCAGCGGCGC+AGG | + | chr6_3:8169057-8169076 | None:intergenic | 60.0% | |
| ! | GGCGGCGGGTTCGTTAGTGT+TGG | + | chr6_3:8169021-8169040 | None:intergenic | 65.0% |
| ! | GGCGGCGGGTTCGTTAGTGT+TGG | + | chr6_3:8169021-8169040 | None:intergenic | 65.0% |
| GCAGGGATGGTCGGAGACGG+CGG | + | chr6_3:8169039-8169058 | None:intergenic | 70.0% | |
| GGCGCAGGGATGGTCGGAGA+CGG | + | chr6_3:8169042-8169061 | None:intergenic | 70.0% | |
| CAATGGCAGCGGCGCAGGGA+TGG | + | chr6_3:8169052-8169071 | None:intergenic | 70.0% | |
| GCAGGGATGGTCGGAGACGG+CGG | + | chr6_3:8169039-8169058 | None:intergenic | 70.0% | |
| GGCGCAGGGATGGTCGGAGA+CGG | + | chr6_3:8169042-8169061 | None:intergenic | 70.0% | |
| CAATGGCAGCGGCGCAGGGA+TGG | + | chr6_3:8169052-8169071 | None:intergenic | 70.0% | |
| GGATGGTCGGAGACGGCGGC+GGG | + | chr6_3:8169035-8169054 | None:intergenic | 75.0% | |
| GGGATGGTCGGAGACGGCGG+CGG | + | chr6_3:8169036-8169055 | None:intergenic | 75.0% | |
| GGCAGCGGCGCAGGGATGGT+CGG | + | chr6_3:8169048-8169067 | None:intergenic | 75.0% | |
| GGATGGTCGGAGACGGCGGC+GGG | + | chr6_3:8169035-8169054 | None:intergenic | 75.0% | |
| GGGATGGTCGGAGACGGCGG+CGG | + | chr6_3:8169036-8169055 | None:intergenic | 75.0% | |
| GGCAGCGGCGCAGGGATGGT+CGG | + | chr6_3:8169048-8169067 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 8168993 | 8169831 | 8168993 | ID=Msa0937090;Name=Msa0937090 |
| chr6_3 | mRNA | 8168993 | 8169831 | 8168993 | ID=Msa0937090-mRNA-1;Parent=Msa0937090;Name=Msa0937090-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|0|0|2|0|93 |
| chr6_3 | exon | 8169741 | 8169831 | 8169741 | ID=Msa0937090-mRNA-1:exon:2675;Parent=Msa0937090-mRNA-1 |
| chr6_3 | exon | 8168993 | 8169180 | 8168993 | ID=Msa0937090-mRNA-1:exon:2674;Parent=Msa0937090-mRNA-1 |
| chr6_3 | CDS | 8169741 | 8169831 | 8169741 | ID=Msa0937090-mRNA-1:cds;Parent=Msa0937090-mRNA-1 |
| chr6_3 | CDS | 8168993 | 8169180 | 8168993 | ID=Msa0937090-mRNA-1:cds;Parent=Msa0937090-mRNA-1 |
| Gene Sequence |
| Protein sequence |
