Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0940980 | sp|Q9FWQ5|HAC12_ARATH | 44.681 | 94 | 47 | 1 | 3 | 91 | 1599 | 1692 | 3.05e-22 | 92.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0940980 | A0A396HCL0 | 70.642 | 109 | 19 | 2 | 1 | 99 | 671 | 776 | 1.86e-39 | 149 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0940980 | TR | TAZ |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0940980 | MtrunA17_Chr6g0456861 | 70.642 | 109 | 19 | 2 | 1 | 99 | 671 | 776 | 3.57e-43 | 149 |
| Msa0940980 | MtrunA17_Chr3g0133461 | 68.224 | 107 | 26 | 1 | 1 | 99 | 1237 | 1343 | 4.20e-43 | 149 |
| Msa0940980 | MtrunA17_Chr3g0138411 | 65.421 | 107 | 29 | 1 | 1 | 99 | 1100 | 1206 | 2.18e-42 | 147 |
| Msa0940980 | MtrunA17_Chr5g0438991 | 76.923 | 91 | 13 | 1 | 1 | 83 | 868 | 958 | 4.41e-42 | 145 |
| Msa0940980 | MtrunA17_Chr4g0000031 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1602 | 1695 | 4.58e-22 | 89.0 |
| Msa0940980 | MtrunA17_Chr5g0401281 | 38.235 | 68 | 41 | 1 | 16 | 82 | 283 | 350 | 4.21e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0940980 | AT1G16710.3 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1573 | 1666 | 3.07e-23 | 92.8 |
| Msa0940980 | AT1G16710.10 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1573 | 1666 | 3.07e-23 | 92.8 |
| Msa0940980 | AT1G16710.8 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1573 | 1666 | 3.07e-23 | 92.8 |
| Msa0940980 | AT1G16710.7 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1573 | 1666 | 3.07e-23 | 92.8 |
| Msa0940980 | AT1G16710.1 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1599 | 1692 | 3.10e-23 | 92.8 |
| Msa0940980 | AT1G16710.2 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1570 | 1663 | 3.19e-23 | 92.8 |
| Msa0940980 | AT1G16710.6 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1534 | 1627 | 3.55e-23 | 92.4 |
| Msa0940980 | AT1G16710.4 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1534 | 1627 | 3.55e-23 | 92.4 |
| Msa0940980 | AT1G16710.5 | 44.681 | 94 | 47 | 1 | 3 | 91 | 1534 | 1627 | 3.55e-23 | 92.4 |
| Msa0940980 | AT1G79000.1 | 46.809 | 94 | 45 | 2 | 3 | 91 | 1590 | 1683 | 4.67e-23 | 92.4 |
| Msa0940980 | AT1G79000.3 | 46.809 | 94 | 45 | 2 | 3 | 91 | 1566 | 1659 | 4.99e-23 | 92.0 |
| Msa0940980 | AT1G79000.2 | 46.809 | 94 | 45 | 2 | 3 | 91 | 1590 | 1683 | 5.05e-23 | 92.0 |
| Msa0940980 | AT3G12980.1 | 37.895 | 95 | 51 | 2 | 2 | 91 | 1564 | 1655 | 1.52e-14 | 68.2 |
| Msa0940980 | AT3G12980.2 | 37.895 | 95 | 51 | 2 | 2 | 91 | 1483 | 1574 | 1.55e-14 | 68.2 |
| Msa0940980 | AT5G67480.2 | 42.029 | 69 | 39 | 1 | 16 | 83 | 288 | 356 | 2.41e-12 | 61.6 |
| Msa0940980 | AT5G67480.1 | 42.029 | 69 | 39 | 1 | 16 | 83 | 277 | 345 | 2.45e-12 | 61.6 |
| Msa0940980 | AT5G67480.3 | 42.029 | 69 | 39 | 1 | 16 | 83 | 277 | 345 | 2.45e-12 | 61.6 |
| Msa0940980 | AT1G55970.2 | 35.802 | 81 | 49 | 1 | 11 | 91 | 1366 | 1443 | 3.68e-11 | 58.5 |
| Msa0940980 | AT1G55970.1 | 35.802 | 81 | 49 | 1 | 11 | 91 | 1380 | 1457 | 3.95e-11 | 58.2 |
| Msa0940980 | AT4G37610.1 | 42.424 | 66 | 36 | 2 | 19 | 82 | 273 | 338 | 5.82e-11 | 57.8 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCGAGTCTCTGCAATTCC+TGG | 0.327710 | 6_3:+14127372 | None:intergenic |
| TTGTCGTAGAGCTGAAGTTT+TGG | 0.361135 | 6_3:-14127149 | Msa0940980:CDS |
| AATGTAGAATACCGCGTTGT+AGG | 0.362248 | 6_3:-14127351 | Msa0940980:intron |
| AAGTGCAAAATTCGAATGAA+TGG | 0.438598 | 6_3:-14127442 | Msa0940980:CDS |
| AGTGCAAAATTCGAATGAAT+GGG | 0.457982 | 6_3:-14127441 | Msa0940980:CDS |
| GAAAGCGACGGAGCAGTCAT+TGG | 0.464537 | 6_3:-14127127 | Msa0940980:CDS |
| TTATACTAGCTACGCACTCC+AGG | 0.495911 | 6_3:-14127390 | Msa0940980:CDS |
| CTCCAGGAATTGCAGAGACT+CGG | 0.528433 | 6_3:-14127374 | Msa0940980:CDS |
| GTGCAAAATTCGAATGAATG+GGG | 0.623329 | 6_3:-14127440 | Msa0940980:CDS |
| TGCAAAATTCGAATGAATGG+GGG | 0.645250 | 6_3:-14127439 | Msa0940980:CDS |
| ATTGTAACACACCTACAACG+CGG | 0.798908 | 6_3:+14127340 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACCATATCTTTTTACAATGT+TGG | + | chr6_3:14127242-14127261 | None:intergenic | 25.0% |
| ! | GTAGAATATCAAAATTCTCT+AGG | + | chr6_3:14127353-14127372 | None:intergenic | 25.0% |
| AAGTGCAAAATTCGAATGAA+TGG | - | chr6_3:14127210-14127229 | Msa0940980:intron | 30.0% | |
| AGTGCAAAATTCGAATGAAT+GGG | - | chr6_3:14127211-14127230 | Msa0940980:intron | 30.0% | |
| GCCAACATTGTAAAAAGATA+TGG | - | chr6_3:14127238-14127257 | Msa0940980:intron | 30.0% | |
| ! | CATTCGAATTTTGCACTTAC+TGG | + | chr6_3:14127208-14127227 | None:intergenic | 35.0% |
| GTGCAAAATTCGAATGAATG+GGG | - | chr6_3:14127212-14127231 | Msa0940980:intron | 35.0% | |
| TGCAAAATTCGAATGAATGG+GGG | - | chr6_3:14127213-14127232 | Msa0940980:intron | 35.0% | |
| AATGTAGAATACCGCGTTGT+AGG | - | chr6_3:14127301-14127320 | Msa0940980:intron | 40.0% | |
| ATTGTAACACACCTACAACG+CGG | + | chr6_3:14127315-14127334 | None:intergenic | 40.0% | |
| ! | TTGTCGTAGAGCTGAAGTTT+TGG | - | chr6_3:14127503-14127522 | Msa0940980:CDS | 40.0% |
| TTATACTAGCTACGCACTCC+AGG | - | chr6_3:14127262-14127281 | Msa0940980:intron | 45.0% | |
| !!! | GCTGAAGTTTTGGAAAGCGA+CGG | - | chr6_3:14127513-14127532 | Msa0940980:CDS | 45.0% |
| CTCCAGGAATTGCAGAGACT+CGG | - | chr6_3:14127278-14127297 | Msa0940980:intron | 50.0% | |
| TTCCGAGTCTCTGCAATTCC+TGG | + | chr6_3:14127283-14127302 | None:intergenic | 50.0% | |
| GAAAGCGACGGAGCAGTCAT+TGG | - | chr6_3:14127525-14127544 | Msa0940980:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 14127120 | 14127554 | 14127120 | ID=Msa0940980;Name=Msa0940980 |
| chr6_3 | mRNA | 14127120 | 14127554 | 14127120 | ID=Msa0940980-mRNA-1;Parent=Msa0940980;Name=Msa0940980-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0.5|2|0|99 |
| chr6_3 | exon | 14127352 | 14127554 | 14127352 | ID=Msa0940980-mRNA-1:exon:4470;Parent=Msa0940980-mRNA-1 |
| chr6_3 | exon | 14127120 | 14127216 | 14127120 | ID=Msa0940980-mRNA-1:exon:4469;Parent=Msa0940980-mRNA-1 |
| chr6_3 | CDS | 14127352 | 14127554 | 14127352 | ID=Msa0940980-mRNA-1:cds;Parent=Msa0940980-mRNA-1 |
| chr6_3 | CDS | 14127120 | 14127216 | 14127120 | ID=Msa0940980-mRNA-1:cds;Parent=Msa0940980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
