AlfalfaGEDB | Msa0943350

M. sativa cultivar zhongmu-4 / Msa0943350

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943350	sp\|Q9SZL8\|FRS5_ARATH	45.489	266	143	1	98	363	374	637	1.15e-74	255

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943350	A0A2Z6PSQ9	53.516	512	147	11	1	470	6	468	6.10e-167	492

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TFs/TRs:

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Gene ID	Type	Classification
Msa0943350	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943350	MtrunA17_Chr1g0202341	65.205	342	114	3	81	419	332	671	2.05e-157	465
Msa0943350	MtrunA17_Chr1g0202341	57.426	101	39	1	1	97	14	114	4.99e-26	112
Msa0943350	MtrunA17_Chr5g0448761	43.357	286	154	2	98	383	365	642	5.26e-75	253
Msa0943350	MtrunA17_Chr5g0448511	36.184	304	192	1	98	401	137	438	5.13e-68	229
Msa0943350	MtrunA17_Chr3g0118611	39.163	263	156	2	98	360	243	501	2.78e-57	204
Msa0943350	MtrunA17_Chr5g0440451	36.029	272	170	2	89	360	337	604	6.55e-52	190
Msa0943350	MtrunA17_Chr2g0296781	34.737	380	202	13	89	432	282	651	8.82e-52	188
Msa0943350	MtrunA17_Chr6g0477261	33.687	377	208	11	98	440	180	548	4.34e-51	185
Msa0943350	MtrunA17_Chr1g0207551	36.765	272	169	2	89	360	321	589	1.33e-50	186
Msa0943350	MtrunA17_Chr5g0441581	37.868	272	157	7	98	362	319	585	5.04e-50	183

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943350	AT4G38180.1	45.489	266	143	1	98	363	374	637	1.17e-75	255
Msa0943350	AT4G38170.1	34.591	318	194	6	89	401	130	438	7.21e-58	202
Msa0943350	AT2G27110.3	34.708	291	184	3	89	379	167	451	2.79e-53	192
Msa0943350	AT2G27110.2	34.708	291	184	3	89	379	312	596	1.12e-52	192
Msa0943350	AT2G27110.1	34.708	291	184	3	89	379	312	596	1.12e-52	192
Msa0943350	AT4G15090.1	34.035	285	184	3	89	372	341	622	1.70e-52	192
Msa0943350	AT4G15090.5	34.035	285	184	3	89	372	341	622	1.70e-52	192
Msa0943350	AT4G15090.3	34.035	285	184	3	89	372	341	622	1.70e-52	192
Msa0943350	AT4G15090.2	34.035	285	184	3	89	372	341	622	1.70e-52	192
Msa0943350	AT4G15090.4	34.035	285	184	3	89	372	341	622	1.70e-52	192

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Find 74 sgRNAs with CRISPR-Local

Find 504 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGATTTAGTTTCCGTTT+GGG	0.421062	6_3:+19013178	Msa0943350:CDS
AAATGATTACGCACATAAGC+AGG	0.530436	6_3:-19014260	None:intergenic
AACTTTCTCCTTTGCATCAA+TGG	0.302581	6_3:-19014783	None:intergenic
AAGCAATGATTGATAAATAT+GGG	0.540447	6_3:+19014177	Msa0943350:CDS
AAGTATGAATAAGTTCTTTA+AGG	0.149127	6_3:+19014316	Msa0943350:CDS
AATCTTCAACAGTTGTAGAT+TGG	0.417126	6_3:-19014139	None:intergenic
ACAAACTCCATTCCTTCATA+GGG	0.549722	6_3:-19013109	None:intergenic
ACAAATTCTTTGTTCAAAAG+AGG	0.356383	6_3:+19013243	Msa0943350:CDS
ACAAGGTATGCAAAGAACAA+AGG	0.624420	6_3:+19013160	Msa0943350:CDS
ACCCTAATGGTTTCACATCT+AGG	0.418321	6_3:-19016944	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCACTATCAATGATAATCA+TGG	-	chr6_3:19013038-19013057	None:intergenic	30.0%
TGTATCATTTGAAGATGCAA+AGG	+	chr6_3:19013129-19013148	Msa0943350:CDS	30.0%
GAGACAAAAAACGTTTGAAA+GGG	+	chr6_3:19013272-19013291	Msa0943350:CDS	30.0%
AGACAAAAAACGTTTGAAAG+GGG	+	chr6_3:19013273-19013292	Msa0943350:CDS	30.0%
AACCAAAAAGATTGGATTGT+TGG	-	chr6_3:19013862-19013881	None:intergenic	30.0%
GATCTGTGATAATAGACTTA+GGG	-	chr6_3:19013965-19013984	None:intergenic	30.0%
TGATCTGTGATAATAGACTT+AGG	-	chr6_3:19013966-19013985	None:intergenic	30.0%
TCTATTATCACAGATCAAGA+CGG	+	chr6_3:19013969-19013988	Msa0943350:intron	30.0%
TATTACTAATGCAGTTGCAA+GGG	+	chr6_3:19013995-19014014	Msa0943350:intron	30.0%
AATCTTCAACAGTTGTAGAT+TGG	-	chr6_3:19014142-19014161	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	19013010	19017096	19013010	ID=Msa0943350;Name=Msa0943350
chr6_3	mRNA	19013010	19017096	19013010	ID=Msa0943350-mRNA-1;Parent=Msa0943350;Name=Msa0943350-mRNA-1;_AED=0.26;_eAED=0.35;_QI=0\|0\|0\|0.75\|1\|1\|4\|0\|546
chr6_3	exon	19013010	19013301	19013010	ID=Msa0943350-mRNA-1:exon:5655;Parent=Msa0943350-mRNA-1
chr6_3	exon	19014009	19014895	19014009	ID=Msa0943350-mRNA-1:exon:5656;Parent=Msa0943350-mRNA-1
chr6_3	exon	19014994	19015214	19014994	ID=Msa0943350-mRNA-1:exon:5657;Parent=Msa0943350-mRNA-1
chr6_3	exon	19016856	19017096	19016856	ID=Msa0943350-mRNA-1:exon:5658;Parent=Msa0943350-mRNA-1
chr6_3	CDS	19013010	19013301	19013010	ID=Msa0943350-mRNA-1:cds;Parent=Msa0943350-mRNA-1
chr6_3	CDS	19014009	19014895	19014009	ID=Msa0943350-mRNA-1:cds;Parent=Msa0943350-mRNA-1
chr6_3	CDS	19014994	19015214	19014994	ID=Msa0943350-mRNA-1:cds;Parent=Msa0943350-mRNA-1
chr6_3	CDS	19016856	19017096	19016856	ID=Msa0943350-mRNA-1:cds;Parent=Msa0943350-mRNA-1

Gene Sequence

>Msa0943350

ATGATAGATGTGCACGAAGACTCTGCCATGATTATCATTGATAGTGGACGTGAAATCGCTAATAATGTGGACCATCACAATATTGTGCAGTCGTTAGAGCCCTATGAAGGAATGGAGTTTGTATCATTTGAAGATGCAAAGGATCACTATACAAGGTATGCAAAGAACAAAGGATTTAGTTTCCGTTTGGGTCGTATTACTAGATCAAGAACAAATGGAATGATAATTGGTCAACAAATTCTTTGTTCAAAAGAGGGATTTCGAGACAAAAAACGTTTGAAAGGGGAAAACATTGCAAGGGTGTTTCCTAATGTTAATCATCATTATTGTATGTGGCACATTGAGAAAAAAATACCAGAGTACTTGAGCAGTATCTATCATGAACATAGTGAATTTAAAAATCAGTTTCACAACTTCATTCACCAATCTACAACTGTTGAAGATTTTGAGTCTGATTGGGAAGCAATGATTGATAAATATGGGTTGCAAGATAATCGATGGCTTCAGAATATATATTCTATTACTATTAGAGAAAAATGGATTCCTGCTTATGTGCGTAATCATTTTTGTGCTGGCATGTCGACTACCCAAAGGAGTGAAAGTATGAATAAGTTCTTTAAGGATTTTTTGAGTTCAAGTACTCCATTGAGTAAGTTTGTGAAACAGTATGAGAAGGCTCTTGATGCACGTTATAACAAGGAAAGAGAGAAGACTTTCAAGACAATAAATTCAAAACCATTTTTGCGAACTTTATATCCCATGGAAAAAGAAGCTTCAAAAAGTTACACACGGAAACTGTTCAAGATTTTTCAGGATGAGTTGGTTGGGTCTCAAATGTTTATTGCAGAGAAAGTTGAGTTTTCCACTGATGTTTCTACATACAAGCTTCATGAAATTTACAAAGAGAAGCCTATCTACTATGCGACTTTTCACATTGCTTCAAAAGAAGCTACTTGCAGTTGTCACATGTTTGAATTTTTAGGTATTCTTTGTAGGCATGTGTTAAATATATTCATAAAGAAAAAGATTTTCTCGCTTCCTTCAAGTTATATTTTACGCCGCTGGACCATTGATGCAAAGGAGAAAGTTAAAGGGCAGATGAGTGAAGAATTGCAGGAAGGAAGCAATAAACCTTCTACTACTTTGTTGTTTAATAGTATTATGATTCATTCTCTACAATCAAATGAAGAGCTTGGCAATTCAACATCTAATGTTATTCCGGAAGTCTCTAATAATGAGATAACTGTGTGTGATCTTCCTGTTGCAAAAAATAAAGGGCGTCCACAATCTTTGCGAATGAAAGGTAGCTTGGAATCTCTTAATAAAGGTTCATTTGCTTGTAGTTATTGCAAGAAGAGAGGTCATAACAAACGTAAATGTCCCGATTTAAAGAAAGCAAGGCCAAAGAACAAATTCTATTTACTATGCATAAATGAACAAACGACATCTTTCTTCATCCTCTGGTTCTCCGACGATTACAAGGCATGTCCTAGATGTGAAACCATTAGGGTGGTGCCTCTGCTGCTCCAAGTCGACGACGCCTTTGCAGCCTACAGAGGAATGACAAAATCTAAGACTCTAACTAAGATCTCTCAAAAATGGATTCTCTTAAGGACACCATACGCATGGTCTGAAATGTGA

Protein sequence

>Msa0943350

MIDVHEDSAMIIIDSGREIANNVDHHNIVQSLEPYEGMEFVSFEDAKDHYTRYAKNKGFSFRLGRITRSRTNGMIIGQQILCSKEGFRDKKRLKGENIARVFPNVNHHYCMWHIEKKIPEYLSSIYHEHSEFKNQFHNFIHQSTTVEDFESDWEAMIDKYGLQDNRWLQNIYSITIREKWIPAYVRNHFCAGMSTTQRSESMNKFFKDFLSSSTPLSKFVKQYEKALDARYNKEREKTFKTINSKPFLRTLYPMEKEASKSYTRKLFKIFQDELVGSQMFIAEKVEFSTDVSTYKLHEIYKEKPIYYATFHIASKEATCSCHMFEFLGILCRHVLNIFIKKKIFSLPSSYILRRWTIDAKEKVKGQMSEELQEGSNKPSTTLLFNSIMIHSLQSNEELGNSTSNVIPEVSNNEITVCDLPVAKNKGRPQSLRMKGSLESLNKGSFACSYCKKRGHNKRKCPDLKKARPKNKFYLLCINEQTTSFFILWFSDDYKACPRCETIRVVPLLLQVDDAFAAYRGMTKSKTLTKISQKWILLRTPYAWSEM*