AlfalfaGEDB | Msa0945830

M. sativa cultivar zhongmu-4 / Msa0945830

KEGG: K04733
GO: GO:0001101 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0008150 GO:0009505 GO:0009506 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0030054 GO:0030312 GO:0031090 GO:0031224 GO:0031225 GO:0031226 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071944 GO:0097305 GO:0098588 GO:0098805 GO:1901700
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0945830	sp\|Q9LP77\|Y1848_ARATH	49.489	489	184	10	24	453	32	516	3.70e-142	426

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0945830	G7K1N3	84.008	494	45	7	1	461	1	493	0.0	781

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0945830	MtrunA17_Chr5g0434551	84.008	494	45	7	1	461	1	493	0.0	781
Msa0945830	MtrunA17_Chr8g0393211	50.298	503	184	11	27	468	35	532	2.33e-146	434
Msa0945830	MtrunA17_Chr6g0478621	47.379	515	174	11	24	453	22	524	6.96e-134	402
Msa0945830	MtrunA17_Chr4g0062911	43.011	465	200	15	28	441	30	480	3.70e-95	301
Msa0945830	MtrunA17_Chr3g0128551	38.664	494	240	13	7	452	11	489	3.19e-90	288
Msa0945830	MtrunA17_Chr5g0432851	39.197	523	253	16	3	476	17	523	5.45e-89	285
Msa0945830	MtrunA17_Chr8g0387791	38.182	495	231	13	11	456	5	473	2.68e-81	265
Msa0945830	MtrunA17_Chr3g0126271	37.425	497	229	15	4	451	3	466	6.92e-81	263
Msa0945830	MtrunA17_Chr1g0159161	36.495	485	241	16	5	464	4	446	6.55e-76	249
Msa0945830	MtrunA17_Chr7g0245171	38.398	487	232	15	15	453	16	482	1.80e-75	249

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0945830	AT1G48480.1	49.489	489	184	10	24	453	32	516	3.76e-143	426
Msa0945830	AT3G17840.1	49.378	482	193	9	23	453	29	510	1.60e-138	414
Msa0945830	AT5G16590.1	47.305	501	212	13	8	468	11	499	1.49e-132	397
Msa0945830	AT3G02880.1	46.436	491	220	9	1	451	1	488	7.81e-128	385
Msa0945830	AT2G26730.1	39.960	498	251	12	11	464	12	505	4.17e-92	294
Msa0945830	AT3G08680.1	40.319	501	227	16	7	452	4	487	2.10e-90	289
Msa0945830	AT3G08680.2	40.319	501	227	16	7	452	4	487	2.10e-90	289
Msa0945830	AT5G58300.3	37.449	494	237	14	23	464	43	516	6.89e-84	272
Msa0945830	AT5G58300.1	37.449	494	237	14	23	464	43	516	6.89e-84	272
Msa0945830	AT5G58300.2	37.449	494	237	14	23	464	43	516	6.89e-84	272

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Find 152 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTTCAGCTGAAGTTT+TGG	0.268030	6_3:+25069602	Msa0945830:CDS
AAACCTCATTATTTCAATAT+TGG	0.267757	6_3:+25071895	Msa0945830:three_prime_UTR
AAACCTCTTCGGAATTTCTC+CGG	0.500931	6_3:-25069172	None:intergenic
AAACGTAGCAGTAAATTAAC+TGG	0.402342	6_3:-25071977	None:intergenic
AAAGCAGACGTCTATAGCTT+TGG	0.451446	6_3:+25071472	Msa0945830:CDS
AAAGGAGGAACATTAAGATC+AGG	0.541154	6_3:-25069117	None:intergenic
AACAACTTATAAGGCAACAT+TGG	0.530845	6_3:+25069641	Msa0945830:CDS
AAGAGTACGTCCACCCACCG+TGG	0.742353	6_3:-25068698	None:intergenic
AAGGAGTAGACCTACCAAGA+TGG	0.552958	6_3:+25071554	Msa0945830:three_prime_UTR
AATCCCTTGAGCAGCACCAA+GGG	0.602496	6_3:-25071258	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGCAAAATCCATAGGAAT+TGG	-	chr6_3:25068898-25068917	None:intergenic	30.0%
TTTATACGGTTTGCAGAATT+TGG	+	chr6_3:25068981-25069000	Msa0945830:intron	30.0%
TGTGAGTAGAAAGTTTAGTT+TGG	+	chr6_3:25069569-25069588	Msa0945830:CDS	30.0%
AACAACTTATAAGGCAACAT+TGG	+	chr6_3:25069641-25069660	Msa0945830:CDS	30.0%
CTAAAATAATAACCCCTAAG+TGG	-	chr6_3:25069777-25069796	None:intergenic	30.0%
TGTGTATGATTATATGCCAA+TGG	+	chr6_3:25069815-25069834	Msa0945830:CDS	30.0%
GTGTATGATTATATGCCAAT+GGG	+	chr6_3:25069816-25069835	Msa0945830:CDS	30.0%
GAGACAATGATTAACACATT+CGG	+	chr6_3:25070291-25070310	Msa0945830:intron	30.0%
ATAGCAAACTAAAATCAGAC+AGG	-	chr6_3:25070382-25070401	None:intergenic	30.0%
GACAGAATTGATAGATATTC+TGG	+	chr6_3:25070612-25070631	Msa0945830:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	25068589	25072081	25068589	ID=Msa0945830;Name=Msa0945830
chr6_3	mRNA	25068589	25072081	25068589	ID=Msa0945830-mRNA-1;Parent=Msa0945830;Name=Msa0945830-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0.14\|0.12\|1\|0.14\|0.12\|8\|582\|491
chr6_3	exon	25068589	25068835	25068589	ID=Msa0945830-mRNA-1:exon:7101;Parent=Msa0945830-mRNA-1
chr6_3	exon	25068842	25068988	25068842	ID=Msa0945830-mRNA-1:exon:7102;Parent=Msa0945830-mRNA-1
chr6_3	exon	25069019	25069128	25069019	ID=Msa0945830-mRNA-1:exon:7103;Parent=Msa0945830-mRNA-1
chr6_3	exon	25069147	25069367	25069147	ID=Msa0945830-mRNA-1:exon:7104;Parent=Msa0945830-mRNA-1
chr6_3	exon	25069372	25069531	25069372	ID=Msa0945830-mRNA-1:exon:7105;Parent=Msa0945830-mRNA-1
chr6_3	exon	25069549	25069783	25069549	ID=Msa0945830-mRNA-1:exon:7106;Parent=Msa0945830-mRNA-1
chr6_3	exon	25069802	25069861	25069802	ID=Msa0945830-mRNA-1:exon:7107;Parent=Msa0945830-mRNA-1
chr6_3	exon	25071204	25072081	25071204	ID=Msa0945830-mRNA-1:exon:7108;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25068589	25068835	25068589	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25068842	25068988	25068842	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25069019	25069128	25069019	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25069147	25069367	25069147	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25069372	25069531	25069372	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25069549	25069783	25069549	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25069802	25069861	25069802	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	CDS	25071204	25071499	25071204	ID=Msa0945830-mRNA-1:cds;Parent=Msa0945830-mRNA-1
chr6_3	three_prime_UTR	25071500	25072081	25071500	ID=Msa0945830-mRNA-1:three_prime_utr;Parent=Msa0945830-mRNA-1

Gene Sequence

>Msa0945830

ATGAAGCTCAAAAAAACCGTTCTGCTTTATTTCACAGCATGTTTAATAATCACCATTGTTTCCGGCGCCGATCTAGCTTCCGACAGAGCCAGTTTATTGACACTCCGCGCCACGGTGGGTGGACGTACTCTTCTATGGAATTCATCAGAAACAAACCCATGTTTATGGACCGGAGTTATCTGCAATAACAAAAGAGTCACTGCCTTAAGGTTACCGGCGATGGGTCTCTCCGGTAACCTTCCTTCAGGTAATCTTACTGAATTGCAGACACTTTCTCTCCGGTATAACGCATTAACAGGACCAATTCCTATGGATTTTGCAAAACTGGTTTCACTTAGAAATCTTTATCTTCATAGTAACTTTTTCTCCGGTGAGGTGCCGGAGTTTTTATACGGTAAAAACAATTTCAGTGGTGAAATTTCTGAACATTTTAATAATCTTACTCGTTTGGATACATTGTTTTTGGAACAGAACATGTTTACTGGTTCTGTTCCTGATCTTAATTTCAATGTATCGTTTAATAATTTAACCGGAGAAATTCCGAAGAGGTTTTCGCGCTTAAATGTAAGTGCTTTTTCTGGGAATTTGCTTTGTGGGAATCCTCTTCAAGTAGCTTGTCCTGGAAACAATGACAAAAATGGTTTATCTGGTGGAGCTATTGCTGGTATTGTGATTGGTTGTGTTTTTCGGTTTGGTGTTGATTCTTGTTCTTTTGGTGTTGTGTTAAAAAGAAAAAAAAGTGATTCTGATAATGTAGCACGTGCCAAGAGTGTTGAAGGTGAGGTGTCACGTGAGAAAACTAGAGATTTTGAGAGTGGTGGTGGTGGAGTTATTCAGGTTTGGCTTCGGCTTCAACGACGGCTTCAACTTCGGTTTCAGCTTCTGAGTTTGATATTTATTGGGAATGTGAGTAGAAAGTTTAGTTTGGATGATTTGTTAAAAGCTTCAGCTGAAGTTTTGGGAAAAGGGACATTTGGAACAACTTATAAGGCAACATTGGAGATGGGAATAAGTGTGGCTGTGAAGAGGTTGAAGGATGTTACAGCAAGTGAAAGAGAATTCAGGGAGAAAATTGAGGAGGTTGGGAAATTGGTTCATGAGAAATTGGTCCCACTTAGGGATGAGAAGCTTGTTGTGTATGATTATATGCCAATGGGAAGCTTATCTGCACTCTTGCATGCAAATAATGGAGCTGGAAGGACTCCACTGAATTGGGAAACTAGGTCAACCATTGCCCTTGGTGCTGCTCAAGGGATTGCATACCTACACTCACAAGGTCCAACATCTTCCCATGGAAACATCAAGTCATCAAACATCCTTCTCACCAAATCATTTGAACCTCGCGTCTCAGATTTGGCCTCGCTTACCTGGCTCTTCCAACCGCTACACCCAACCGAGTTTCCGGTTACCGTGCACCAGAGGTCACCGATGCGCGCAAAGTATCCCAAAAAGCAGACGTCTATAGCTTTGGTATAA

Protein sequence

>Msa0945830

MKLKKTVLLYFTACLIITIVSGADLASDRASLLTLRATVGGRTLLWNSSETNPCLWTGVICNNKRVTALRLPAMGLSGNLPSGNLTELQTLSLRYNALTGPIPMDFAKLVSLRNLYLHSNFFSGEVPEFLYGKNNFSGEISEHFNNLTRLDTLFLEQNMFTGSVPDLNFNVSFNNLTGEIPKRFSRLNVSAFSGNLLCGNPLQVACPGNNDKNGLSGGAIAGIVIGCVFRFGVDSCSFGVVLKRKKSDSDNVARAKSVEGEVSREKTRDFESGGGGVIQVWLRLQRRLQLRFQLLSLIFIGNVSRKFSLDDLLKASAEVLGKGTFGTTYKATLEMGISVAVKRLKDVTASEREFREKIEEVGKLVHEKLVPLRDEKLVVYDYMPMGSLSALLHANNGAGRTPLNWETRSTIALGAAQGIAYLHSQGPTSSHGNIKSSNILLTKSFEPRVSDLASLTWLFQPLHPTEFPVTVHQRSPMRAKYPKKQTSIALV*