AlfalfaGEDB | Msa0957670

M. sativa cultivar zhongmu-4 / Msa0957670

KEGG: -
GO: -
PFAM: PF01657.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0957670	XP_039691123.1	35.345	232	130	7	58	277	28	251	3.27e-32	143
Msa0957670	XP_039691123.1	84.211	513	63	2	18	513	221	732	0.0	887

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0957670	sp\|Q9M0X5\|CRK25_ARATH	43.699	492	246	9	49	513	25	512	1.21e-125	385

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0957670	MtrunA17_Chr6g0474901	84.211	513	63	2	18	513	221	732	0.0	887
Msa0957670	MtrunA17_Chr6g0474901	35.345	232	130	7	58	277	28	251	3.01e-36	143
Msa0957670	MtrunA17_Chr6g0475091	82.946	516	69	2	15	513	234	747	0.0	857
Msa0957670	MtrunA17_Chr6g0475091	36.842	247	134	9	42	272	28	268	6.19e-37	145
Msa0957670	MtrunA17_Chr6g0474811	79.696	527	84	5	5	513	218	739	0.0	839
Msa0957670	MtrunA17_Chr6g0474811	31.271	291	168	9	50	324	25	299	3.83e-32	131
Msa0957670	MtrunA17_Chr6g0474871	80.418	526	77	6	5	513	215	731	0.0	828
Msa0957670	MtrunA17_Chr6g0474871	31.119	286	170	6	50	324	25	294	1.34e-32	132
Msa0957670	MtrunA17_Chr6g0475001	83.736	455	55	3	18	455	228	680	0.0	783
Msa0957670	MtrunA17_Chr6g0475001	35.124	242	135	7	50	277	25	258	2.11e-38	149

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0957670	AT4G05200.1	43.699	492	246	9	49	513	25	512	1.23e-126	385
Msa0957670	AT4G05200.2	43.699	492	246	9	49	513	43	530	2.16e-126	385
Msa0957670	AT4G23160.1	41.104	489	263	7	47	513	39	524	8.13e-125	380
Msa0957670	AT4G23180.3	43.381	491	244	10	47	513	33	513	1.64e-122	369
Msa0957670	AT4G23180.2	43.381	491	244	10	47	513	33	513	2.98e-121	368
Msa0957670	AT4G23180.1	43.381	491	244	10	47	513	33	513	4.57e-120	367
Msa0957670	AT4G23230.1	43.852	488	235	11	48	513	32	502	2.67e-118	362
Msa0957670	AT4G23140.1	40.763	498	260	7	47	513	23	516	1.33e-115	356
Msa0957670	AT4G23130.3	40.612	490	259	7	49	513	27	509	1.68e-115	353
Msa0957670	AT4G23140.2	40.763	498	260	7	47	513	23	516	2.17e-115	356

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Find 135 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGCAGAAGAAGCAGC+AGG	0.606388	6_3:-69687546	Msa0957670:five_prime_UTR
AAAACTTCACGTGCATTTCC+TGG	0.373577	6_3:+69686531	None:intergenic
AAACTGTCTTTCATGTCCAA+AGG	0.553597	6_3:-69686994	Msa0957670:CDS
AAAGGGGAAGAAAGAGTCAT+AGG	0.402530	6_3:-69686359	Msa0957670:CDS
AAAGTGGGAAAGGATAACAG+AGG	0.754510	6_3:+69685344	None:intergenic
AAAGTTCCATACTGTTGAAG+TGG	0.509358	6_3:-69687111	Msa0957670:CDS
AAATCCAGGAATTGTATCAA+TGG	0.514014	6_3:+69687638	None:intergenic
AACGACAAAAGTTGTTAACT+TGG	0.437515	6_3:-69685596	Msa0957670:CDS
AACTGTCTTTCATGTCCAAA+GGG	0.486209	6_3:-69686993	Msa0957670:CDS
AAGGACAATTACATTGGCAA+AGG	0.614752	6_3:-69686125	Msa0957670:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCCAGGAATTGTATCAA+TGG	+	chr6_3:69685875-69685894	None:intergenic	30.0%
GCATTAAGAGACCTATAAAA+AGG	+	chr6_3:69686208-69686227	None:intergenic	30.0%
TGTTGTCCCTACAATTATTT+CGG	-	chr6_3:69687092-69687111	Msa0957670:CDS	30.0%
TCACTATATCTCAATTGAAG+AGG	-	chr6_3:69687513-69687532	Msa0957670:five_prime_UTR	30.0%
TAGATTTCTATGTTGAAGCT+TGG	+	chr6_3:69687700-69687719	None:intergenic	30.0%
AGAAATCTAGTGGCATTTAT+TGG	-	chr6_3:69687711-69687730	Msa0957670:five_prime_UTR	30.0%
AACGACAAAAGTTGTTAACT+TGG	-	chr6_3:69687914-69687933	Msa0957670:five_prime_UTR	30.0%
ACGACAAAAGTTGTTAACTT+GGG	-	chr6_3:69687915-69687934	Msa0957670:five_prime_UTR	30.0%
ACCTTGTCATCAAAATCCAT+AGG	-	chr6_3:69685628-69685647	Msa0957670:intron	35.0%
AGAATCAGTTCGATTTACAC+CGG	+	chr6_3:69685683-69685702	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	69685341	69688191	69685341	ID=Msa0957670;Name=Msa0957670
chr6_3	mRNA	69685341	69688191	69685341	ID=Msa0957670-mRNA-1;Parent=Msa0957670;Name=Msa0957670-mRNA-1;_AED=0.14;_eAED=0.14;_QI=753\|1\|1\|1\|0.8\|0.66\|6\|45\|513
chr6_3	exon	69685341	69685624	69685341	ID=Msa0957670-mRNA-1:exon:12702;Parent=Msa0957670-mRNA-1
chr6_3	exon	69685722	69685932	69685722	ID=Msa0957670-mRNA-1:exon:12701;Parent=Msa0957670-mRNA-1
chr6_3	exon	69686103	69686224	69686103	ID=Msa0957670-mRNA-1:exon:12700;Parent=Msa0957670-mRNA-1
chr6_3	exon	69686340	69686462	69686340	ID=Msa0957670-mRNA-1:exon:12699;Parent=Msa0957670-mRNA-1
chr6_3	exon	69686541	69687240	69686541	ID=Msa0957670-mRNA-1:exon:12698;Parent=Msa0957670-mRNA-1
chr6_3	exon	69687292	69688191	69687292	ID=Msa0957670-mRNA-1:exon:12697;Parent=Msa0957670-mRNA-1
chr6_3	five_prime_UTR	69687439	69688191	69687439	ID=Msa0957670-mRNA-1:five_prime_utr;Parent=Msa0957670-mRNA-1
chr6_3	CDS	69687292	69687438	69687292	ID=Msa0957670-mRNA-1:cds;Parent=Msa0957670-mRNA-1
chr6_3	CDS	69686541	69687240	69686541	ID=Msa0957670-mRNA-1:cds;Parent=Msa0957670-mRNA-1
chr6_3	CDS	69686340	69686462	69686340	ID=Msa0957670-mRNA-1:cds;Parent=Msa0957670-mRNA-1
chr6_3	CDS	69686103	69686224	69686103	ID=Msa0957670-mRNA-1:cds;Parent=Msa0957670-mRNA-1
chr6_3	CDS	69685722	69685932	69685722	ID=Msa0957670-mRNA-1:cds;Parent=Msa0957670-mRNA-1
chr6_3	CDS	69685386	69685624	69685386	ID=Msa0957670-mRNA-1:cds;Parent=Msa0957670-mRNA-1
chr6_3	three_prime_UTR	69685341	69685385	69685341	ID=Msa0957670-mRNA-1:three_prime_utr;Parent=Msa0957670-mRNA-1

Gene Sequence

>Msa0957670

ATGATTGCAGAACATGCCTCGGTCGAGCTATTAGGGAACTTTCATATGTATGATGGACAACAAGGTGGGAGAAATTTATATCCTAGTTGTAATATTAGGTATGAACTATATCCTTTTTATAGGTCTCTTAATGCTCCTTCACCAAATGATCCAGTTTATCTTTCCTATAATTGCTCAAGAAACCACAGTACAATCACTAACAAAAACTTCAAATTACTTCTCTCTTATTTGTCCTCCAACGCCACAAATGGCAAAAAGTTCCATACTGTTGAAGTGGAAGAAATGCTGTATGGCCTCTTCATGTGTCGAGGTGACCTTTCCGTTCGCCTCTGTGGTCGATGTGTGAAAAATTCAACCGATCAAATATACTCAAACTGTCTTTCATGTCCAAAGGGTATAATTTGGTACAGCCACTGCTTGGTTCGCTATTCAGATAAAAATATCTTCTCTAACATCGAAACAAGTCCTATGTATAGAGATATAAATATCACCACATATTCCATGACATACGAAAACTTGTTCACTAGTACATTATCAAATCAGTTATCTCAACTTGCGAATGATACAGGGAACAGTGATGAAAGGTATAAGACTGATTCATTAAAACTGAATGATGTACAAACCTTGTATACTCTTGGACAATGTACGCGAGGTTTGTCTAGTCAGGATTGCGCTTCTTGTCTAAATGATGTCATCGTTTCAGCAATTCCATGGTCAAATTTGGGTAGTGTTGGGGGAAGAATTATATATCCTAGCTGCAATCTGAGGTTTGAATTGTTCCGATTTTACATGGAGGGCGACGAACCTCGGCTGTCTGGGAGTCCTTCTCCATTACCAGGAAATGCACAGAAACGGAAAATCGTCTTCATTGTTGTCCCTACAATTATTTCGGTGATGCTATTTTCTATTGGCTACTATTGGCTAAAGAAAAGGGGAAGAAAGAGTCATAGGACTATTCTTCGAGAAAATTTTGGTGAAGAAAGTGTCACTTTAGAGCCATTGCGATTCGACTGGGTGGTAATTGAAGCAGCAACCAACAACTTCTCTAAGGACAATTACATTGGCAAAGGCGGGTTTGGAGAGGTTTACAAGGGTATCCTTCTTGATGGAAGAGAAGTGGCTATAAAAAGGCTTTCAAAAAGTTCTAAGCAAGGTGTAGAAGAGTTCAAGAATGAAGTTTTATTGATAGCCAAGCTTCAACATAGAAATCTAGTGGCATTTATTGGTTTTTGCCTTGAAGAACATGAGAAAATACTTATTTATGAATTTGTGCCCAACAAAAGTCTTGATTTCTTTCTATTTGATTCTCAACGACAAAAGTTGTTAACTTGGGTTGAACGCTTCAATATCATTGGAGGAATTGTTCGAGGAATTCTTTATTTGCATGAACATTCTCGACTCAAAGTTATACATCGCGATCTCAAACCTAGCAATATTTTATTAGACAAAAATATGATTCCTAAAATTTCAGATTTTGGTTTGGCTCGAATTGTGGAAATAAGTCAAGATGAGGGAAGTACAAAGAGAATTGTTGGAACATAG

Protein sequence

>Msa0957670

MIAEHASVELLGNFHMYDGQQGGRNLYPSCNIRYELYPFYRSLNAPSPNDPVYLSYNCSRNHSTITNKNFKLLLSYLSSNATNGKKFHTVEVEEMLYGLFMCRGDLSVRLCGRCVKNSTDQIYSNCLSCPKGIIWYSHCLVRYSDKNIFSNIETSPMYRDINITTYSMTYENLFTSTLSNQLSQLANDTGNSDERYKTDSLKLNDVQTLYTLGQCTRGLSSQDCASCLNDVIVSAIPWSNLGSVGGRIIYPSCNLRFELFRFYMEGDEPRLSGSPSPLPGNAQKRKIVFIVVPTIISVMLFSIGYYWLKKRGRKSHRTILRENFGEESVTLEPLRFDWVVIEAATNNFSKDNYIGKGGFGEVYKGILLDGREVAIKRLSKSSKQGVEEFKNEVLLIAKLQHRNLVAFIGFCLEEHEKILIYEFVPNKSLDFFLFDSQRQKLLTWVERFNIIGGIVRGILYLHEHSRLKVIHRDLKPSNILLDKNMIPKISDFGLARIVEISQDEGSTKRIVGT*