AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0958820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0958820 MtrunA17_Chr6g0476301 83.095 349 42 3 91 429 1 342 0.0 573
Msa0958820 MtrunA17_Chr6g0473191 85.135 296 35 1 91 377 1 296 3.01e-175 501
Msa0958820 MtrunA17_Chr6g0476291 86.296 270 37 0 91 360 1 270 3.47e-164 471
Msa0958820 MtrunA17_Chr3g0118361 42.457 464 218 13 42 467 51 503 9.86e-112 350
Msa0958820 MtrunA17_Chr5g0440591 50.779 321 147 4 4 318 14 329 3.02e-109 343
Msa0958820 MtrunA17_Chr3g0080401 48.907 366 145 9 91 443 1 337 6.51e-109 333
Msa0958820 MtrunA17_Chr2g0329151 53.184 267 114 3 32 290 27 290 5.57e-96 308
Msa0958820 MtrunA17_Chr3g0124291 51.163 258 104 6 43 287 32 280 1.52e-86 278
Msa0958820 MtrunA17_Chr8g0382561 46.816 267 133 5 29 287 6 271 1.94e-77 254
Msa0958820 MtrunA17_Chr7g0260281 47.692 65 34 0 80 144 1 65 2.00e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0958820 AT3G20770.1 39.406 505 242 11 31 493 37 519 5.26e-107 338
Msa0958820 AT2G27050.1 39.673 489 218 11 31 472 36 494 1.03e-105 333
Msa0958820 AT1G73730.2 51.493 268 117 3 31 290 14 276 1.10e-91 295
Msa0958820 AT1G73730.1 51.493 268 117 3 31 290 26 288 1.92e-91 295
Msa0958820 AT5G10120.1 49.615 260 123 3 36 287 16 275 1.60e-87 282
Msa0958820 AT5G65100.1 46.263 281 119 7 38 291 33 308 1.44e-79 264
Msa0958820 AT5G21120.3 46.667 255 125 3 48 291 53 307 6.21e-74 248
Msa0958820 AT5G21120.1 46.667 255 125 3 48 291 53 307 6.21e-74 248
Msa0958820 AT5G21120.2 46.667 255 125 3 48 291 60 314 6.72e-74 248

Find 128 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAATGGTTGTTTCCTTAA+TGG 0.114788 6_3:+74878859 None:intergenic
CAAATCCAGCCATTTGAAAT+TGG 0.195986 6_3:+74878465 None:intergenic
TTCAGCAATTCGAATGTAAT+TGG 0.211719 6_3:+74879187 None:intergenic
TTCTCACATTTCTCTCTTGC+TGG 0.213662 6_3:+74878552 None:intergenic
CGGAGATTTCCATTGGAAAA+AGG 0.266811 6_3:-74879097 Msa0958820:CDS
AGCTTCGCCGCTGCTGATTA+TGG 0.273266 6_3:-74877556 Msa0958820:CDS
GCTAAATATGAAGCAGAAAA+TGG 0.280618 6_3:-74879247 Msa0958820:CDS
CCATTTGAGGCTGCATGTTT+TGG 0.280907 6_3:+74877095 None:intergenic
ACAAGATAAACTCACCATTA+AGG 0.283993 6_3:-74878873 Msa0958820:CDS
AGTAATTACCTGCATTGTTA+TGG 0.296901 6_3:+74877530 None:intergenic
AGATCATGAGGCTTTCTATA+AGG 0.298040 6_3:+74878995 None:intergenic
CTTTGATTGACACCGTGATT+TGG 0.301125 6_3:+74877331 None:intergenic
ATTAATTATTGATTCCCTTT+TGG 0.303897 6_3:+74876852 None:intergenic
AACAATGCAAAACAAGAAAA+AGG 0.304941 6_3:-74879871 Msa0958820:CDS
CTTGTTGACCATAGAAATTT+TGG 0.306348 6_3:+74877071 None:intergenic
CTTGTTCTGTTGAACACTTC+CGG 0.312860 6_3:+74877288 None:intergenic
ATGCATTCCAATGTTTATAA+TGG 0.315010 6_3:-74879928 Msa0958820:CDS
TTGATGGACGTATATAATTC+AGG 0.326235 6_3:-74877481 Msa0958820:CDS
ATGAACATGGTTTCAACTTC+CGG 0.329437 6_3:-74877124 Msa0958820:CDS
TTCCTAATCTTCTGAATGTT+AGG 0.342602 6_3:+74878920 None:intergenic
GATCATGAGGCTTTCTATAA+GGG 0.348589 6_3:+74878996 None:intergenic
CCTCCACCATGGTGGCCAAC+TGG 0.352128 6_3:-74879070 Msa0958820:CDS
GGTTTGGAAGGTTTATGTTT+TGG 0.353709 6_3:-74878966 Msa0958820:CDS
TTTGAAGCTTCTCCCCTTGA+AGG 0.355072 6_3:+74879892 None:intergenic
GATGCTCGTGGCTTCATCTA+TGG 0.357885 6_3:-74879373 Msa0958820:CDS
AGAAGTGGTTTCTGATTTGA+TGG 0.358184 6_3:-74877497 Msa0958820:CDS
GCGGCACTAATGATGATAGA+AGG 0.375221 6_3:+74879137 None:intergenic
GTTGAAACCATGTTCATAAC+TGG 0.381589 6_3:+74877130 None:intergenic
TGATAGAAGGGAACTTAATA+TGG 0.382620 6_3:+74879150 None:intergenic
GTGCACAAGAGCGTGTATTA+AGG 0.383295 6_3:-74879684 Msa0958820:intron
GAACACTTGCATGGTAGATT+TGG 0.390194 6_3:+74877386 None:intergenic
AACAACCAATTTCAAATGGC+TGG 0.404663 6_3:-74878470 Msa0958820:CDS
CAAATGGACCAAAATTTCTA+TGG 0.408363 6_3:-74877079 Msa0958820:CDS
ATGAGATTTAGTGAAGATCT+TGG 0.413274 6_3:-74879025 Msa0958820:CDS
CTCCTAACATTCAGAAGATT+AGG 0.416065 6_3:-74878922 Msa0958820:CDS
ATCATCGTCATCGTCATATG+AGG 0.416418 6_3:-74878651 Msa0958820:CDS
CCAAAACATGCAGCCTCAAA+TGG 0.416883 6_3:-74877095 Msa0958820:CDS
GTGCTATCGCTAACCCTTCA+AGG 0.426255 6_3:-74879906 Msa0958820:CDS
TTTGCTTGCATTGGAACATA+AGG 0.426343 6_3:+74877016 None:intergenic
TTTCTCAACGTTCGACCAAA+AGG 0.429941 6_3:-74876867 Msa0958820:CDS
CTACCATGCAAGTGTTCAAC+AGG 0.437195 6_3:-74877380 Msa0958820:CDS
CTCATGATCTGAAGAAGGTT+TGG 0.440824 6_3:-74878982 Msa0958820:CDS
AAGAGACAACACTACATCAT+TGG 0.447664 6_3:-74879734 Msa0958820:CDS
AACAAGAGAAAGCGTGTACT+TGG 0.454094 6_3:-74878623 Msa0958820:CDS
CCATTAAGGAAACAACCATT+TGG 0.457496 6_3:-74878859 Msa0958820:CDS
CAGGCACATGTTAAAGATGA+TGG 0.466945 6_3:-74879404 Msa0958820:CDS
CACCATGATTCTTTCCCAGT+TGG 0.468071 6_3:+74879055 None:intergenic
TTGTCTGATGAACCACTCAT+TGG 0.468990 6_3:+74879328 None:intergenic
GTTGAGGTATTTGCTTGCAT+TGG 0.470104 6_3:+74877007 None:intergenic
CGACAACAACCAATTTCAAA+TGG 0.471802 6_3:-74878474 Msa0958820:CDS
GTTGTCGTTGTTGTTGATGT+TGG 0.474985 6_3:+74878490 None:intergenic
GAACACCATCATGAGTACTA+TGG 0.478356 6_3:-74877266 Msa0958820:CDS
ACAAATTATGAAAAGTTGAA+TGG 0.493728 6_3:-74879214 Msa0958820:CDS
CATCAGACAATTTACGAGGC+TGG 0.493913 6_3:-74879315 Msa0958820:CDS
CTCCACCATGGTGGCCAACT+GGG 0.496691 6_3:-74879069 Msa0958820:CDS
ATTAATAATAGCAAACCAAA+TGG 0.502082 6_3:+74878844 None:intergenic
AAGGGAACTTAATATGGTAT+CGG 0.502090 6_3:+74879156 None:intergenic
CAAAATTTCTATGGTCAACA+AGG 0.508752 6_3:-74877070 Msa0958820:CDS
TCTTTCCCAGTTGGCCACCA+TGG 0.508797 6_3:+74879064 None:intergenic
AGGGAACTTAATATGGTATC+GGG 0.509697 6_3:+74879157 None:intergenic
CCAGTTGGCCACCATGGTGG+AGG 0.511934 6_3:+74879070 None:intergenic
AATGGAAATCTCCGTTGTGG+TGG 0.514931 6_3:+74879106 None:intergenic
TTGAAGCTTCTCCCCTTGAA+GGG 0.515863 6_3:+74879893 None:intergenic
TTCTCAACGTTCGACCAAAA+GGG 0.517578 6_3:-74876866 Msa0958820:CDS
ACCACAACGGAGATTTCCAT+TGG 0.522988 6_3:-74879104 Msa0958820:CDS
TGAATCAAAGGTTGTTGTTG+AGG 0.525190 6_3:+74876991 None:intergenic
TTATGATTAACACCGGGAGT+TGG 0.525458 6_3:+74877418 None:intergenic
CAAACCTTCTTCAGATCATG+AGG 0.527892 6_3:+74878983 None:intergenic
CACTTGCATGGTAGATTTGG+TGG 0.539023 6_3:+74877389 None:intergenic
TCTCCTGTTGAACACTTGCA+TGG 0.543277 6_3:+74877377 None:intergenic
TGATCTGAAGAAGGTTTGGA+AGG 0.544263 6_3:-74878978 Msa0958820:CDS
CTCATTGGTTTGCCCTCGTG+AGG 0.546764 6_3:+74879343 None:intergenic
TCATATTTAGCAATTGCCGC+TGG 0.547452 6_3:+74879259 None:intergenic
TATGGATTCCATAACAATGC+AGG 0.549065 6_3:-74877538 Msa0958820:CDS
GGCCAACTGGGAAAGAATCA+TGG 0.559080 6_3:-74879057 Msa0958820:CDS
ATGATCAAAAGATGAATCAA+AGG 0.559551 6_3:+74876979 None:intergenic
GCTTGCACACGTTGGTCAAG+AGG 0.560353 6_3:+74878413 None:intergenic
TTCCCAGTTGGCCACCATGG+TGG 0.564701 6_3:+74879067 None:intergenic
TCCAATGGAAATCTCCGTTG+TGG 0.567968 6_3:+74879103 None:intergenic
CATCATTGAACTATTCATCA+TGG 0.573478 6_3:+74877165 None:intergenic
AAAGCCTCATGATCTGAAGA+AGG 0.573569 6_3:-74878987 Msa0958820:CDS
TGCTATCGCTAACCCTTCAA+GGG 0.574060 6_3:-74879905 Msa0958820:CDS
AAGTAATTATGTGCTTGTTG+AGG 0.574583 6_3:-74878753 Msa0958820:CDS
AACAATGCAGGTAATTACTC+AGG 0.576216 6_3:-74877526 Msa0958820:CDS
TTCAATGCCAGTTATGAACA+TGG 0.587129 6_3:-74877137 Msa0958820:CDS
ATCATGAGGCTTTCTATAAG+GGG 0.587351 6_3:+74878997 None:intergenic
CGGCACTAATGATGATAGAA+GGG 0.589144 6_3:+74879138 None:intergenic
CACTTTACTAGTACACAACT+AGG 0.591972 6_3:-74878521 Msa0958820:CDS
ACACTACATCATTGGAACAA+AGG 0.595301 6_3:-74879726 Msa0958820:CDS
ATTTATTGACTCTCCTACTG+TGG 0.596370 6_3:-74876906 Msa0958820:CDS
ATGGAAATCTCCGTTGTGGT+GGG 0.597513 6_3:+74879107 None:intergenic
CTATGGAGTAATACCTCACG+AGG 0.599594 6_3:-74879356 Msa0958820:CDS
ACAAAGGAAAAGAAAGACAA+TGG 0.610942 6_3:-74879710 Msa0958820:CDS
GCTAACTATTGAGGAACTAG+AGG 0.611138 6_3:-74879812 Msa0958820:CDS
GATAGCACCATTATAAACAT+TGG 0.611287 6_3:+74879921 None:intergenic
GTGCATGTTATGATTAACAC+CGG 0.611942 6_3:+74877411 None:intergenic
TAACTTGATGATGCCACAGT+AGG 0.612606 6_3:+74876893 None:intergenic
AGTACGATGATTCCAACTCC+CGG 0.614991 6_3:-74877430 Msa0958820:CDS
GGAACATTGTCAAACAGTCA+AGG 0.625113 6_3:-74878901 Msa0958820:CDS
GACGATGACGATGATGATGA+TGG 0.627984 6_3:+74878659 None:intergenic
CAGACAATTTACGAGGCTGG+TGG 0.628174 6_3:-74879312 Msa0958820:CDS
TCATCGTCATCGTCATATGA+GGG 0.630319 6_3:-74878650 Msa0958820:CDS
AAAATTTCTATGGTCAACAA+GGG 0.632348 6_3:-74877069 Msa0958820:CDS
TTACTCAGGAGGAGAAGAAG+TGG 0.633102 6_3:-74877512 Msa0958820:CDS
AAATTTCTATGGTCAACAAG+GGG 0.633963 6_3:-74877068 Msa0958820:CDS
TGGAACCATAGTACTCATGA+TGG 0.643063 6_3:+74877261 None:intergenic
TGGAATTCATGTTGATCAAG+AGG 0.645230 6_3:+74878572 None:intergenic
TGGAATCATCGTACTCATGA+CGG 0.645856 6_3:+74877438 None:intergenic
CGTTGTGGTGGGTCGCAATG+CGG 0.647311 6_3:+74879118 None:intergenic
AGTACTATGATTCCAAATCA+CGG 0.648005 6_3:-74877343 Msa0958820:CDS
CGCATTGCGACCCACCACAA+CGG 0.648689 6_3:-74879117 Msa0958820:CDS
ATGGAAGTTTGTGATGCTCG+TGG 0.649462 6_3:-74879385 Msa0958820:CDS
AATGCAGGTAATTACTCAGG+AGG 0.654047 6_3:-74877523 Msa0958820:CDS
TGACCAACGTGTGCAAGCTG+CGG 0.662625 6_3:-74878408 Msa0958820:intron
ATGCATCGTCAAATCTACAC+CGG 0.672329 6_3:-74877307 Msa0958820:CDS
CACGAGGGCAAACCAATGAG+TGG 0.675933 6_3:-74879340 Msa0958820:CDS
TCATGAGGCTTTCTATAAGG+GGG 0.678672 6_3:+74878998 None:intergenic
ATGGAATCCATAATCAGCAG+CGG 0.681546 6_3:+74877549 None:intergenic
AATTTCTATGGTCAACAAGG+GGG 0.688284 6_3:-74877067 Msa0958820:CDS
TGCATGTTATGATTAACACC+GGG 0.698948 6_3:+74877412 None:intergenic
GCTATCGCTAACCCTTCAAG+GGG 0.702578 6_3:-74879904 Msa0958820:CDS
AAGGCATTCCTCCACCATGG+TGG 0.706316 6_3:-74879078 Msa0958820:CDS
ACACTTGAAGTGTAGACTTG+AGG 0.709845 6_3:+74877211 None:intergenic
CAAGTGTTCATGAACAACAG+AGG 0.720196 6_3:-74877195 Msa0958820:CDS
GGTTCATCAGACAATTTACG+AGG 0.727611 6_3:-74879319 Msa0958820:CDS
GTACCGCAGCTTGCACACGT+TGG 0.738322 6_3:+74878405 None:intergenic
CAACTGGGAAAGAATCATGG+TGG 0.757558 6_3:-74879054 Msa0958820:CDS
TATGGAGTAATACCTCACGA+GGG 0.784582 6_3:-74879355 Msa0958820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGGTATTTTTATTTAAAT+AGG - chr6_3:74878597-74878616 Msa0958820:CDS 10.0%
!!! TAAATGATATATTAGCTTAT+AGG - chr6_3:74878546-74878565 Msa0958820:CDS 15.0%
!! TCTTAATTATTATGAATTGT+AGG + chr6_3:74878729-74878748 None:intergenic 15.0%
!! CTACTTCATTATATATTATT+TGG - chr6_3:74878891-74878910 Msa0958820:CDS 15.0%
!! GATTCAAATCTCATTTAAAA+CGG + chr6_3:74877231-74877250 None:intergenic 20.0%
!! ATAGTTAAATTCGACAAAAA+CGG - chr6_3:74877498-74877517 Msa0958820:CDS 20.0%
!! ACAAATTATGAAAAGTTGAA+TGG - chr6_3:74877567-74877586 Msa0958820:CDS 20.0%
!! ATTAATAATAGCAAACCAAA+TGG + chr6_3:74877940-74877959 None:intergenic 20.0%
!! ATAGGAAAATGTTAGATTAT+AGG - chr6_3:74878615-74878634 Msa0958820:CDS 20.0%
!!! CCATAATTATTTTTCTTTTG+GGG + chr6_3:74878845-74878864 None:intergenic 20.0%
!!! GCCATAATTATTTTTCTTTT+GGG + chr6_3:74878846-74878865 None:intergenic 20.0%
!!! TGCCATAATTATTTTTCTTT+TGG + chr6_3:74878847-74878866 None:intergenic 20.0%
! ATGCATTCCAATGTTTATAA+TGG - chr6_3:74876853-74876872 Msa0958820:CDS 25.0%
! AACAATGCAAAACAAGAAAA+AGG - chr6_3:74876910-74876929 Msa0958820:CDS 25.0%
! GTAAAGTTCTCAAAATCTTA+TGG + chr6_3:74877310-74877329 None:intergenic 25.0%
! TCAAAACTTGAGAAAAATTC+AGG + chr6_3:74877993-74878012 None:intergenic 25.0%
! TAAACAAATGAATAAGTGTG+TGG + chr6_3:74878456-74878475 None:intergenic 25.0%
! GATTCAAGTCATAAATCAAA+TGG + chr6_3:74878505-74878524 None:intergenic 25.0%
! TGCTTGTTTCAAACTTAAAA+AGG - chr6_3:74878579-74878598 Msa0958820:CDS 25.0%
!! TAGATTATAGGTCTCAATTT+TGG - chr6_3:74878627-74878646 Msa0958820:CDS 25.0%
! AAGCTAACAATATTGTAAGA+TGG + chr6_3:74878690-74878709 None:intergenic 25.0%
!! TCACAATTTGTTTATGAATC+TGG - chr6_3:74878783-74878802 Msa0958820:CDS 25.0%
!! CACAATTTGTTTATGAATCT+GGG - chr6_3:74878784-74878803 Msa0958820:CDS 25.0%
!! ACAATTTGTTTATGAATCTG+GGG - chr6_3:74878785-74878804 Msa0958820:CDS 25.0%
! CCCCAAAAGAAAAATAATTA+TGG - chr6_3:74878842-74878861 Msa0958820:CDS 25.0%
! AAAATTTCTATGGTCAACAA+GGG - chr6_3:74879712-74879731 Msa0958820:CDS 25.0%
! ATGATCAAAAGATGAATCAA+AGG + chr6_3:74879805-74879824 None:intergenic 25.0%
GATAGCACCATTATAAACAT+TGG + chr6_3:74876863-74876882 None:intergenic 30.0%
TGCAAAACAAGAAAAAGGAA+AGG - chr6_3:74876915-74876934 Msa0958820:CDS 30.0%
ACAAGAAAAAGGAAAGGAAA+AGG - chr6_3:74876921-74876940 Msa0958820:CDS 30.0%
GAAAAAAGAGCTAACTATTG+AGG - chr6_3:74876960-74876979 Msa0958820:CDS 30.0%
AATGCTGCTAAAAAAGATGA+AGG - chr6_3:74877011-74877030 Msa0958820:CDS 30.0%
ACAAAGGAAAAGAAAGACAA+TGG - chr6_3:74877071-74877090 Msa0958820:CDS 30.0%
!! TTTTGAGAACTTTACCTTTG+AGG - chr6_3:74877315-74877334 Msa0958820:CDS 30.0%
GCTAAATATGAAGCAGAAAA+TGG - chr6_3:74877534-74877553 Msa0958820:CDS 30.0%
TTCAGCAATTCGAATGTAAT+TGG + chr6_3:74877597-74877616 None:intergenic 30.0%
AAGGGAACTTAATATGGTAT+CGG + chr6_3:74877628-74877647 None:intergenic 30.0%
TGATAGAAGGGAACTTAATA+TGG + chr6_3:74877634-74877653 None:intergenic 30.0%
ATGAGATTTAGTGAAGATCT+TGG - chr6_3:74877756-74877775 Msa0958820:intron 30.0%
TTCCTAATCTTCTGAATGTT+AGG + chr6_3:74877864-74877883 None:intergenic 30.0%
ACAAGATAAACTCACCATTA+AGG - chr6_3:74877908-74877927 Msa0958820:intron 30.0%
!!! AGTTTTGATTCTTGTGTTGA+AGG - chr6_3:74878005-74878024 Msa0958820:intron 30.0%
! AAGTAATTATGTGCTTGTTG+AGG - chr6_3:74878028-74878047 Msa0958820:intron 30.0%
!! GGAAAATGATTTTGATGTTG+TGG - chr6_3:74878049-74878068 Msa0958820:intron 30.0%
!! AATGATTTTGATGTTGTGGA+AGG - chr6_3:74878053-74878072 Msa0958820:intron 30.0%
AATCAGATCATTCATCATAC+AGG - chr6_3:74878407-74878426 Msa0958820:intron 30.0%
ATTCATCATACAGGTAATCA+TGG - chr6_3:74878416-74878435 Msa0958820:CDS 30.0%
!! TGACTCATGCTTTAGAAAAT+AGG - chr6_3:74878751-74878770 Msa0958820:CDS 30.0%
! GCTACTTCAAACAATTTTGT+TGG - chr6_3:74878913-74878932 Msa0958820:CDS 30.0%
! AGCTAACACAATTTTTACGA+GGG + chr6_3:74879209-74879228 None:intergenic 30.0%
! AAGCTAACACAATTTTTACG+AGG + chr6_3:74879210-74879229 None:intergenic 30.0%
AGTAATTACCTGCATTGTTA+TGG + chr6_3:74879254-74879273 None:intergenic 30.0%
! TTGATGGACGTATATAATTC+AGG - chr6_3:74879300-74879319 Msa0958820:CDS 30.0%
AGTACTATGATTCCAAATCA+CGG - chr6_3:74879438-74879457 Msa0958820:intron 30.0%
!!! TGCAAGTTTTGATTAACACT+AGG + chr6_3:74879549-74879568 None:intergenic 30.0%
CATCATTGAACTATTCATCA+TGG + chr6_3:74879619-74879638 None:intergenic 30.0%
!! TAGAAATTTTGGTCCATTTG+AGG + chr6_3:74879702-74879721 None:intergenic 30.0%
CAAATGGACCAAAATTTCTA+TGG - chr6_3:74879702-74879721 Msa0958820:CDS 30.0%
! CTTGTTGACCATAGAAATTT+TGG + chr6_3:74879713-74879732 None:intergenic 30.0%
CAAAATTTCTATGGTCAACA+AGG - chr6_3:74879711-74879730 Msa0958820:CDS 30.0%
AAATTTCTATGGTCAACAAG+GGG - chr6_3:74879713-74879732 Msa0958820:CDS 30.0%
AAGAGACAACACTACATCAT+TGG - chr6_3:74877047-74877066 Msa0958820:CDS 35.0%
ACACTACATCATTGGAACAA+AGG - chr6_3:74877055-74877074 Msa0958820:CDS 35.0%
CAAGATCACAAGTTAACTCA+TGG - chr6_3:74877272-74877291 Msa0958820:CDS 35.0%
! TTGACTTTGTTTCACCTCAA+AGG + chr6_3:74877332-74877351 None:intergenic 35.0%
AGGGAACTTAATATGGTATC+GGG + chr6_3:74877627-74877646 None:intergenic 35.0%
ATCATGAGGCTTTCTATAAG+GGG + chr6_3:74877787-74877806 None:intergenic 35.0%
GATCATGAGGCTTTCTATAA+GGG + chr6_3:74877788-74877807 None:intergenic 35.0%
AGATCATGAGGCTTTCTATA+AGG + chr6_3:74877789-74877808 None:intergenic 35.0%
! GGTTTGGAAGGTTTATGTTT+TGG - chr6_3:74877815-74877834 Msa0958820:intron 35.0%
CTCCTAACATTCAGAAGATT+AGG - chr6_3:74877859-74877878 Msa0958820:intron 35.0%
! CCAAATGGTTGTTTCCTTAA+TGG + chr6_3:74877925-74877944 None:intergenic 35.0%
CCATTAAGGAAACAACCATT+TGG - chr6_3:74877922-74877941 Msa0958820:intron 35.0%
TGGAATTCATGTTGATCAAG+AGG + chr6_3:74878212-74878231 None:intergenic 35.0%
CACTTTACTAGTACACAACT+AGG - chr6_3:74878260-74878279 Msa0958820:intron 35.0%
CGACAACAACCAATTTCAAA+TGG - chr6_3:74878307-74878326 Msa0958820:intron 35.0%
AACAACCAATTTCAAATGGC+TGG - chr6_3:74878311-74878330 Msa0958820:intron 35.0%
! CAAATCCAGCCATTTGAAAT+TGG + chr6_3:74878319-74878338 None:intergenic 35.0%
! TGAATGATCTGATTTGCAAC+CGG + chr6_3:74878401-74878420 None:intergenic 35.0%
! CTAACACAATTTTTACGAGG+GGG + chr6_3:74879207-74879226 None:intergenic 35.0%
! GCTAACACAATTTTTACGAG+GGG + chr6_3:74879208-74879227 None:intergenic 35.0%
TATGGATTCCATAACAATGC+AGG - chr6_3:74879243-74879262 Msa0958820:CDS 35.0%
AACAATGCAGGTAATTACTC+AGG - chr6_3:74879255-74879274 Msa0958820:CDS 35.0%
! AGAAGTGGTTTCTGATTTGA+TGG - chr6_3:74879284-74879303 Msa0958820:CDS 35.0%
!! CTCATGACGGTGTTTTTATT+CGG + chr6_3:74879333-74879352 None:intergenic 35.0%
TGCATGTTATGATTAACACC+GGG + chr6_3:74879372-74879391 None:intergenic 35.0%
GTGCATGTTATGATTAACAC+CGG + chr6_3:74879373-74879392 None:intergenic 35.0%
!! TTTTGATTAACACTAGGCGT+TGG + chr6_3:74879543-74879562 None:intergenic 35.0%
TTCAATGCCAGTTATGAACA+TGG - chr6_3:74879644-74879663 Msa0958820:intron 35.0%
GTTGAAACCATGTTCATAAC+TGG + chr6_3:74879654-74879673 None:intergenic 35.0%
ATGAACATGGTTTCAACTTC+CGG - chr6_3:74879657-74879676 Msa0958820:intron 35.0%
AATTTCTATGGTCAACAAGG+GGG - chr6_3:74879714-74879733 Msa0958820:CDS 35.0%
TTTGCTTGCATTGGAACATA+AGG + chr6_3:74879768-74879787 None:intergenic 35.0%
! TGAATCAAAGGTTGTTGTTG+AGG + chr6_3:74879793-74879812 None:intergenic 35.0%
!! ATTTATTGACTCTCCTACTG+TGG - chr6_3:74879875-74879894 Msa0958820:CDS 35.0%
GCTAACTATTGAGGAACTAG+AGG - chr6_3:74876969-74876988 Msa0958820:CDS 40.0%
GATTTGAATCCTGTCTCTTG+AGG - chr6_3:74877241-74877260 Msa0958820:CDS 40.0%
AGCTAACACCTAATGTGAAC+AGG - chr6_3:74877358-74877377 Msa0958820:CDS 40.0%
AACATGTGCCTGTTCACATT+AGG + chr6_3:74877369-74877388 None:intergenic 40.0%
CAGGCACATGTTAAAGATGA+TGG - chr6_3:74877377-74877396 Msa0958820:CDS 40.0%
TATGGAGTAATACCTCACGA+GGG - chr6_3:74877426-74877445 Msa0958820:CDS 40.0%
TTGTCTGATGAACCACTCAT+TGG + chr6_3:74877456-74877475 None:intergenic 40.0%
GGTTCATCAGACAATTTACG+AGG - chr6_3:74877462-74877481 Msa0958820:CDS 40.0%
TCATATTTAGCAATTGCCGC+TGG + chr6_3:74877525-74877544 None:intergenic 40.0%
CGGCACTAATGATGATAGAA+GGG + chr6_3:74877646-74877665 None:intergenic 40.0%
CGGAGATTTCCATTGGAAAA+AGG - chr6_3:74877684-74877703 Msa0958820:intron 40.0%
TCATGAGGCTTTCTATAAGG+GGG + chr6_3:74877786-74877805 None:intergenic 40.0%
AAAGCCTCATGATCTGAAGA+AGG - chr6_3:74877794-74877813 Msa0958820:intron 40.0%
CAAACCTTCTTCAGATCATG+AGG + chr6_3:74877801-74877820 None:intergenic 40.0%
CTCATGATCTGAAGAAGGTT+TGG - chr6_3:74877799-74877818 Msa0958820:intron 40.0%
TGATCTGAAGAAGGTTTGGA+AGG - chr6_3:74877803-74877822 Msa0958820:intron 40.0%
GGAACATTGTCAAACAGTCA+AGG - chr6_3:74877880-74877899 Msa0958820:intron 40.0%
!! GATTTTGATGTTGTGGAAGG+CGG - chr6_3:74878056-74878075 Msa0958820:intron 40.0%
ATCATCGTCATCGTCATATG+AGG - chr6_3:74878130-74878149 Msa0958820:intron 40.0%
TCATCGTCATCGTCATATGA+GGG - chr6_3:74878131-74878150 Msa0958820:intron 40.0%
! AACAAGAGAAAGCGTGTACT+TGG - chr6_3:74878158-74878177 Msa0958820:intron 40.0%
TTCTCACATTTCTCTCTTGC+TGG + chr6_3:74878232-74878251 None:intergenic 40.0%
! GTTGTCGTTGTTGTTGATGT+TGG + chr6_3:74878294-74878313 None:intergenic 40.0%
CAAATGAATAAGTGTGTGGC+TGG + chr6_3:74878452-74878471 None:intergenic 40.0%
GGTGAATCCTTCACATCAAT+GGG - chr6_3:74878806-74878825 Msa0958820:CDS 40.0%
GTGAATCCTTCACATCAATG+GGG - chr6_3:74878807-74878826 Msa0958820:CDS 40.0%
ATGGAATCCATAATCAGCAG+CGG + chr6_3:74879235-74879254 None:intergenic 40.0%
AATGCAGGTAATTACTCAGG+AGG - chr6_3:74879258-74879277 Msa0958820:CDS 40.0%
TGGAATCATCGTACTCATGA+CGG + chr6_3:74879346-74879365 None:intergenic 40.0%
TTATGATTAACACCGGGAGT+TGG + chr6_3:74879366-74879385 None:intergenic 40.0%
GAACACTTGCATGGTAGATT+TGG + chr6_3:74879398-74879417 None:intergenic 40.0%
CTTTGATTGACACCGTGATT+TGG + chr6_3:74879453-74879472 None:intergenic 40.0%
ATGCATCGTCAAATCTACAC+CGG - chr6_3:74879474-74879493 Msa0958820:intron 40.0%
CTTGTTCTGTTGAACACTTC+CGG + chr6_3:74879496-74879515 None:intergenic 40.0%
GAACACCATCATGAGTACTA+TGG - chr6_3:74879515-74879534 Msa0958820:intron 40.0%
TGGAACCATAGTACTCATGA+TGG + chr6_3:74879523-74879542 None:intergenic 40.0%
ACACTTGAAGTGTAGACTTG+AGG + chr6_3:74879573-74879592 None:intergenic 40.0%
CAAGTGTTCATGAACAACAG+AGG - chr6_3:74879586-74879605 Msa0958820:intron 40.0%
!!! CTGCATGTTTTGGCTAAATC+CGG + chr6_3:74879679-74879698 None:intergenic 40.0%
! GTTGAGGTATTTGCTTGCAT+TGG + chr6_3:74879777-74879796 None:intergenic 40.0%
!! TAACTTGATGATGCCACAGT+AGG + chr6_3:74879891-74879910 None:intergenic 40.0%
TTTCTCAACGTTCGACCAAA+AGG - chr6_3:74879914-74879933 Msa0958820:CDS 40.0%
TTCTCAACGTTCGACCAAAA+GGG - chr6_3:74879915-74879934 Msa0958820:CDS 40.0%
TGCTATCGCTAACCCTTCAA+GGG - chr6_3:74876876-74876895 Msa0958820:CDS 45.0%
TTGAAGCTTCTCCCCTTGAA+GGG + chr6_3:74876891-74876910 None:intergenic 45.0%
! TTTGAAGCTTCTCCCCTTGA+AGG + chr6_3:74876892-74876911 None:intergenic 45.0%
! GTGCACAAGAGCGTGTATTA+AGG - chr6_3:74877097-74877116 Msa0958820:CDS 45.0%
ATGGAAGTTTGTGATGCTCG+TGG - chr6_3:74877396-74877415 Msa0958820:CDS 45.0%
CTATGGAGTAATACCTCACG+AGG - chr6_3:74877425-74877444 Msa0958820:CDS 45.0%
CATCAGACAATTTACGAGGC+TGG - chr6_3:74877466-74877485 Msa0958820:CDS 45.0%
ATTCGACAAAAACGGTCCAG+CGG - chr6_3:74877506-74877525 Msa0958820:CDS 45.0%
GCGGCACTAATGATGATAGA+AGG + chr6_3:74877647-74877666 None:intergenic 45.0%
ATGGAAATCTCCGTTGTGGT+GGG + chr6_3:74877677-74877696 None:intergenic 45.0%
AATGGAAATCTCCGTTGTGG+TGG + chr6_3:74877678-74877697 None:intergenic 45.0%
ACCACAACGGAGATTTCCAT+TGG - chr6_3:74877677-74877696 Msa0958820:intron 45.0%
TCCAATGGAAATCTCCGTTG+TGG + chr6_3:74877681-74877700 None:intergenic 45.0%
! GGAGGAATGCCTTTTTCCAA+TGG + chr6_3:74877696-74877715 None:intergenic 45.0%
!! AAAAAGGCATTCCTCCACCA+TGG - chr6_3:74877700-74877719 Msa0958820:intron 45.0%
CACCATGATTCTTTCCCAGT+TGG + chr6_3:74877729-74877748 None:intergenic 45.0%
CAACTGGGAAAGAATCATGG+TGG - chr6_3:74877727-74877746 Msa0958820:intron 45.0%
! GACGATGACGATGATGATGA+TGG + chr6_3:74878125-74878144 None:intergenic 45.0%
GGGTGAATCCTTCACATCAA+TGG - chr6_3:74878805-74878824 Msa0958820:CDS 45.0%
ATGCTACCCCATTGATGTGA+AGG + chr6_3:74878816-74878835 None:intergenic 45.0%
TCACATCAATGGGGTAGCAT+TGG - chr6_3:74878816-74878835 Msa0958820:CDS 45.0%
TTACTCAGGAGGAGAAGAAG+TGG - chr6_3:74879269-74879288 Msa0958820:CDS 45.0%
AGTACGATGATTCCAACTCC+CGG - chr6_3:74879351-74879370 Msa0958820:CDS 45.0%
CACTTGCATGGTAGATTTGG+TGG + chr6_3:74879395-74879414 None:intergenic 45.0%
CTACCATGCAAGTGTTCAAC+AGG - chr6_3:74879401-74879420 Msa0958820:CDS 45.0%
TCTCCTGTTGAACACTTGCA+TGG + chr6_3:74879407-74879426 None:intergenic 45.0%
!! CCATTTGAGGCTGCATGTTT+TGG + chr6_3:74879689-74879708 None:intergenic 45.0%
CCAAAACATGCAGCCTCAAA+TGG - chr6_3:74879686-74879705 Msa0958820:CDS 45.0%
!!! ATTTTATTTTTATTTTAACT+AGG - chr6_3:74877125-74877144 Msa0958820:CDS 5.0%
GTGCTATCGCTAACCCTTCA+AGG - chr6_3:74876875-74876894 Msa0958820:CDS 50.0%
GCTATCGCTAACCCTTCAAG+GGG - chr6_3:74876877-74876896 Msa0958820:CDS 50.0%
GACTACTTGCCTCAAGAGAC+AGG + chr6_3:74877253-74877272 None:intergenic 50.0%
GATGCTCGTGGCTTCATCTA+TGG - chr6_3:74877408-74877427 Msa0958820:CDS 50.0%
CAGACAATTTACGAGGCTGG+TGG - chr6_3:74877469-74877488 Msa0958820:CDS 50.0%
GGCCAACTGGGAAAGAATCA+TGG - chr6_3:74877724-74877743 Msa0958820:intron 50.0%
!! CTCATTGGTTTGCCCTCGTG+AGG + chr6_3:74877441-74877460 None:intergenic 55.0%
CACGAGGGCAAACCAATGAG+TGG - chr6_3:74877441-74877460 Msa0958820:CDS 55.0%
! AAGGCATTCCTCCACCATGG+TGG - chr6_3:74877703-74877722 Msa0958820:intron 55.0%
TCTTTCCCAGTTGGCCACCA+TGG + chr6_3:74877720-74877739 None:intergenic 55.0%
GCTTGCACACGTTGGTCAAG+AGG + chr6_3:74878371-74878390 None:intergenic 55.0%
TGACCAACGTGTGCAAGCTG+CGG - chr6_3:74878373-74878392 Msa0958820:intron 55.0%
! AGCTTCGCCGCTGCTGATTA+TGG - chr6_3:74879225-74879244 Msa0958820:CDS 55.0%
!! CGTTGTGGTGGGTCGCAATG+CGG + chr6_3:74877666-74877685 None:intergenic 60.0%
CGCATTGCGACCCACCACAA+CGG - chr6_3:74877664-74877683 Msa0958820:intron 60.0%
CTCCACCATGGTGGCCAACT+GGG - chr6_3:74877712-74877731 Msa0958820:intron 60.0%
TTCCCAGTTGGCCACCATGG+TGG + chr6_3:74877717-74877736 None:intergenic 60.0%
GTACCGCAGCTTGCACACGT+TGG + chr6_3:74878379-74878398 None:intergenic 60.0%
ACGTGTGCAAGCTGCGGTAC+CGG - chr6_3:74878379-74878398 Msa0958820:intron 60.0%
CCAGTTGGCCACCATGGTGG+AGG + chr6_3:74877714-74877733 None:intergenic 65.0%
CCTCCACCATGGTGGCCAAC+TGG - chr6_3:74877711-74877730 Msa0958820:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 74876853 74879950 74876853 ID=Msa0958820;Name=Msa0958820
chr6_3 mRNA 74876853 74879950 74876853 ID=Msa0958820-mRNA-1;Parent=Msa0958820;Name=Msa0958820-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|3|0|675
chr6_3 exon 74879685 74879950 74879685 ID=Msa0958820-mRNA-1:exon:13365;Parent=Msa0958820-mRNA-1
chr6_3 exon 74878409 74879423 74878409 ID=Msa0958820-mRNA-1:exon:13364;Parent=Msa0958820-mRNA-1
chr6_3 exon 74876853 74877599 74876853 ID=Msa0958820-mRNA-1:exon:13363;Parent=Msa0958820-mRNA-1
chr6_3 CDS 74879685 74879950 74879685 ID=Msa0958820-mRNA-1:cds;Parent=Msa0958820-mRNA-1
chr6_3 CDS 74878409 74879423 74878409 ID=Msa0958820-mRNA-1:cds;Parent=Msa0958820-mRNA-1
chr6_3 CDS 74876853 74877599 74876853 ID=Msa0958820-mRNA-1:cds;Parent=Msa0958820-mRNA-1
Gene Sequence

>Msa0958820

ATGCATTCCAATGTTTATAATGGTGCTATCGCTAACCCTTCAAGGGGAGAAGCTTCAAACAATGCAAAACAAGAAAAAGGAAAGGAAAAGGTAGAAGATGAAATTGAGAAAAAAGAGCTAACTATTGAGGAACTAGAGGCAAAGATATCTAAAGATAAAATGCTGCTAAAAAAGATGAAGGAAGAAAGAAGTAAAAGAGACAACACTACATCATTGGAACAAAGGAAAAGAAAGACAATGGCTCGTGCACAAGAGCGTGTATTAAGGCACATGTTAAAGATGATGGAAGTTTGTGATGCTCGTGGCTTCATCTATGGAGTAATACCTCACGAGGGCAAACCAATGAGTGGTTCATCAGACAATTTACGAGGCTGGTGGAAAGACATAGTTAAATTCGACAAAAACGGTCCAGCGGCAATTGCTAAATATGAAGCAGAAAATGGAATTACAACAACAAATTATGAAAAGTTGAATGGTGAACCAATTACATTCGAATTGCTGAATGAACTACCCGATACCATATTAAGTTCCCTTCTATCATCATTAGTGCCGCATTGCGACCCACCACAACGGAGATTTCCATTGGAAAAAGGCATTCCTCCACCATGGTGGCCAACTGGGAAAGAATCATGGTGGAATGAGATGAGATTTAGTGAAGATCTTGGCATACCCCCTTATAGAAAGCCTCATGATCTGAAGAAGGTTTGGAAGGTTTATGTTTTGGCAGCTGTAATAAAACACATGTCTCCTAACATTCAGAAGATTAGGAACATTGTCAAACAGTCAAGGAATTTACAAGATAAACTCACCATTAAGGAAACAACCATTTGGTTTGCTATTATTAATCATGAAGAAACAATTGCAAGAAAACTACATCCTGAATTTTTCTCAAGTTTTGATTCTTGTGTTGAAGGAAGTAATTATGTGCTTGTTGAGGAAAATGATTTTGATGTTGTGGAAGGCGGTGAGCACAATCTAGCAAAACGAAAACTATCATCATCATCTTCACCATCATCATCATCGTCATCGTCATATGAGGGTACTAACAAGAGAAAGCGTGTACTTGGTAAGAGAATTAGTACTGAACATGCTACACCTCTTGATCAACATGAATTCCAGCAAGAGAGAAATGTGAGAAACAATCATCACTTTACTAGTACACAACTAGGAAGCAGCACCAACATCAACAACAACGACAACAACCAATTTCAAATGGCTGGATTTGAAGTGTCAACAGTTCATCAAAATGTTGCACCTCTTGACCAACGTGTGCAAGCTGCGCCCCCTCGTAAAAATTGTGTTAGCTTCGCCGCTGCTGATTATGGATTCCATAACAATGCAGGTAATTACTCAGGAGGAGAAGAAGTGGTTTCTGATTTGATGGACGTATATAATTCAGGCATTCAGCCGAATAAAAACACCGTCATGAGTACGATGATTCCAACTCCCGGTGTTAATCATAACATGCACCACCAAATCTACCATGCAAGTGTTCAACAGGAGAAGAACAGCATGAGTACTATGATTCCAAATCACGGTGTCAATCAAAGCATGCATCGTCAAATCTACACCGGAAGTGTTCAACAGAACAAGAACACCATCATGAGTACTATGGTTCCAACGCCTAGTGTTAATCAAAACTTGCATCCTCAAGTCTACACTTCAAGTGTTCATGAACAACAGAGGAGCAACACCATGATGAATAGTTCAATGATGAGTACTTCAATGCCAGTTATGAACATGGTTTCAACTTCCGGATTTAGCCAAAACATGCAGCCTCAAATGGACCAAAATTTCTATGGTCAACAAGGGGGTGCAGATAGCTACTATCACTACAAAGTGCCTTATGTTCCAATGCAAGCAAATACCTCAACAACAACCTTTGATTCATCTTTTGATCATTTGAATGCATTCAATTCTCAATCTGATTTTGATGCTTACATCAACTCAGCATTTATTGACTCTCCTACTGTGGCATCATCAAGTTATGATTTCTCAACGTTCGACCAAAAGGGAATCAATAATTAA
Protein sequence

>Msa0958820

MHSNVYNGAIANPSRGEASNNAKQEKGKEKVEDEIEKKELTIEELEAKISKDKMLLKKMKEERSKRDNTTSLEQRKRKTMARAQERVLRHMLKMMEVCDARGFIYGVIPHEGKPMSGSSDNLRGWWKDIVKFDKNGPAAIAKYEAENGITTTNYEKLNGEPITFELLNELPDTILSSLLSSLVPHCDPPQRRFPLEKGIPPPWWPTGKESWWNEMRFSEDLGIPPYRKPHDLKKVWKVYVLAAVIKHMSPNIQKIRNIVKQSRNLQDKLTIKETTIWFAIINHEETIARKLHPEFFSSFDSCVEGSNYVLVEENDFDVVEGGEHNLAKRKLSSSSSPSSSSSSSYEGTNKRKRVLGKRISTEHATPLDQHEFQQERNVRNNHHFTSTQLGSSTNINNNDNNQFQMAGFEVSTVHQNVAPLDQRVQAAPPRKNCVSFAAADYGFHNNAGNYSGGEEVVSDLMDVYNSGIQPNKNTVMSTMIPTPGVNHNMHHQIYHASVQQEKNSMSTMIPNHGVNQSMHRQIYTGSVQQNKNTIMSTMVPTPSVNQNLHPQVYTSSVHEQQRSNTMMNSSMMSTSMPVMNMVSTSGFSQNMQPQMDQNFYGQQGGADSYYHYKVPYVPMQANTSTTTFDSSFDHLNAFNSQSDFDAYINSAFIDSPTVASSSYDFSTFDQKGINN*