AlfalfaGEDB | Msa0960540

M. sativa cultivar zhongmu-4 / Msa0960540

KEGG: K04733
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0960540	sp\|Q9LP77\|Y1848_ARATH	57.252	655	247	12	22	674	32	655	0.0	702

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0960540	A0A072UCA8	95.912	685	19	2	1	685	1	676	0.0	1337

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0960540	MtrunA17_Chr6g0478621	95.912	685	19	2	1	685	1	676	0.0	1337
Msa0960540	MtrunA17_Chr8g0393211	67.428	657	173	9	25	672	35	659	0.0	824
Msa0960540	MtrunA17_Chr5g0434551	53.421	687	259	13	1	681	1	632	0.0	664
Msa0960540	MtrunA17_Chr3g0108301	43.881	670	319	11	1	663	13	632	1.94e-173	510
Msa0960540	MtrunA17_Chr5g0432851	43.862	668	312	13	1	662	22	632	3.49e-170	502
Msa0960540	MtrunA17_Chr3g0128551	42.922	664	311	15	22	673	28	635	5.04e-163	483
Msa0960540	MtrunA17_Chr8g0387791	43.192	639	288	13	40	669	38	610	7.65e-156	464
Msa0960540	MtrunA17_Chr3g0126271	39.201	676	336	10	7	675	6	613	8.80e-149	446
Msa0960540	MtrunA17_Chr1g0209521	43.722	661	318	18	11	658	19	638	2.18e-144	436
Msa0960540	MtrunA17_Chr5g0396941	38.282	687	313	14	3	674	9	599	7.41e-144	432

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0960540	AT1G48480.1	57.252	655	247	12	22	674	32	655	0.0	702
Msa0960540	AT3G17840.1	57.253	648	232	12	22	660	30	641	0.0	677
Msa0960540	AT5G16590.1	50.377	663	277	14	5	665	9	621	0.0	607
Msa0960540	AT3G02880.1	51.201	666	267	14	2	661	6	619	0.0	596
Msa0960540	AT3G08680.1	43.251	689	329	17	1	680	2	637	9.09e-167	493
Msa0960540	AT3G08680.2	43.251	689	329	17	1	680	2	637	9.09e-167	493
Msa0960540	AT5G05160.1	42.136	693	320	20	6	681	12	640	4.29e-150	450
Msa0960540	AT1G68400.1	43.838	641	318	19	25	658	32	637	2.31e-143	434
Msa0960540	AT5G24100.1	38.657	670	333	14	3	659	9	613	7.35e-135	410
Msa0960540	AT2G36570.1	39.815	648	325	16	44	665	45	653	1.39e-132	406

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Find 255 sgRNAs with CRISPR-Local

Find 361 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCGCCGGAAGTTCAC+CGG	0.507469	6_3:+79971616	None:intergenic
AAACTTAAATTATTTGTAAT+TGG	0.111670	6_3:+79968738	None:intergenic
AAATGGATATTCGTCGGCCG+CGG	0.578033	6_3:-79970982	Msa0960540:CDS
AAATTATTTAAAAGTTTAGG+AGG	0.508117	6_3:+79968856	None:intergenic
AACAATGGTTCATGAAAATT+TGG	0.147687	6_3:-79970685	Msa0960540:CDS
AACCCTTGTCCTAAGGAAGG+GGG	0.668984	6_3:-79971323	Msa0960540:CDS
AACGGAGGCTGAGAGTGTGG+AGG	0.705941	6_3:+79971723	None:intergenic
AACTTAAATTATTTGTAATT+GGG	0.338423	6_3:+79968739	None:intergenic
AAGAAAGGGAAATTATCTGG+TGG	0.682756	6_3:-79971188	Msa0960540:CDS
AAGAAGAAAGGGAAATTATC+TGG	0.385486	6_3:-79971191	Msa0960540:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGATTTGAGAGATAAAAAGA+AGG	+	chr6_3:79968530-79968549	None:intergenic	30.0%
GTTTCAAACAACATGCTAAA+CGG	-	chr6_3:79969200-79969219	Msa0960540:CDS	30.0%
AAGAAGAAAGGGAAATTATC+TGG	-	chr6_3:79969428-79969447	Msa0960540:CDS	30.0%
CATAATCAGCTTATTGATGA+AGG	-	chr6_3:79969575-79969594	Msa0960540:CDS	30.0%
TAATGGAAATGGATATTCGT+CGG	-	chr6_3:79969631-79969650	Msa0960540:CDS	30.0%
CTATAATAATAAGCCCTAAG+AGG	+	chr6_3:79969962-79969981	None:intergenic	30.0%
GAAACATGAACAAGCAAAAA+AGG	+	chr6_3:79970074-79970093	None:intergenic	30.0%
CATAAACAAGTGAACTGAAA+TGG	+	chr6_3:79970533-79970552	None:intergenic	30.0%
CCCAAATAGATGTATGTAAA+AGG	+	chr6_3:79970559-79970578	None:intergenic	30.0%
ATGTTTCCATGAGAAACATT+AGG	+	chr6_3:79970947-79970966	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	79968437	79972204	79968437	ID=Msa0960540;Name=Msa0960540
chr6_3	mRNA	79968437	79972204	79968437	ID=Msa0960540-mRNA-1;Parent=Msa0960540;Name=Msa0960540-mRNA-1;_AED=0.00;_eAED=0.00;_QI=190\|1\|1\|1\|1\|1\|2\|721\|685
chr6_3	exon	79968437	79969795	79968437	ID=Msa0960540-mRNA-1:exon:14384;Parent=Msa0960540-mRNA-1
chr6_3	exon	79970595	79972204	79970595	ID=Msa0960540-mRNA-1:exon:14383;Parent=Msa0960540-mRNA-1
chr6_3	five_prime_UTR	79972015	79972204	79972015	ID=Msa0960540-mRNA-1:five_prime_utr;Parent=Msa0960540-mRNA-1
chr6_3	CDS	79970595	79972014	79970595	ID=Msa0960540-mRNA-1:cds;Parent=Msa0960540-mRNA-1
chr6_3	CDS	79969158	79969795	79969158	ID=Msa0960540-mRNA-1:cds;Parent=Msa0960540-mRNA-1
chr6_3	three_prime_UTR	79968437	79969157	79968437	ID=Msa0960540-mRNA-1:three_prime_utr;Parent=Msa0960540-mRNA-1

Gene Sequence

>Msa0960540

ATGAAGTTTTATTTCTCATTCTTTTTCTTTTTTCTGACACTTTTTCTTCCGTCGGTAAAACCCGACCTCACTTCCGAACGCGCCGCCTTATTAACCCTACGCGCCGCCGTTACCGGCCGAACTCTACTCTGGAACACTACCTCCGCCTCTCCCTGCAACTGGGCTGGCGTCCATTGTAACCAAAACCACACTCACGTTGTCGAGCTTCATCTCCCCGCCGTCGCACTCTCCGGCAACCTCCCCGCCGGAGTATTCTCCGAACTCCCTAACCTCCACACTCTCAGCCTCCGTTTCAACTCTCTCTCCGGTCCTCTCCCCTCCGACCTTTCTTCTTGCACCTCCCTCAAAAACCTCTACCTTCAGCAAAATCTTCTCTCCGGTGAACTTCCGGCGACTTTTTTCAACCTCACCGGACTTGTTCGCCTGAACTTAGCTTCAAATAATTTCTCCGGTGAAATTCCGGTGGGGTTCAACAACCTAACCCGGTTGAAAACATTGTATCTTCAAGACAACCGGTTCACCGGTTCGTTAAGCGAGTTTGAGTCAAACCCGGTTCAACTCGCTCAGTTCAATGTTTCAAACAACATGCTAAACGGTTCTGTTCCTGAAAAGTTGCAAACTTTTGGTAAAGATTCGTTTTTGGGTAATTTGTTATGTGGGAAACCACTTAACCCTTGTCCTAAGGAAGGGGGTGCAAATAGTGGAAAAGGGGGTGCAAATAGTGGTAATGGGGGTAGAAATAGTGGAAATGGGGGTAGAAATTCAAGTGTGGTTGATGATAATAATGGGTTTGTGCGGAACAAGAAGAAAGGGAAATTATCTGGTGGTGCTATAGCTGGAATTGTTATTGGGTCAGTTGTGATTCTTTTGCTTGTGGTTTTTGCTTTGATTGTTTTATGTAGGAATAGAAATGGTGAAAAAATTGATGAAGTTGCAAGAACTTTGAAGCATAATCAGCTTATTGATGAAGGGATTCATGGTGAGAATGTTGGTCATGGTAATGGTAATGGAAATGGATATTCGTCGGCCGCGGCTATGACAGTTGCGGCACCGATGAATGGTGAGAGAAGTAGTGAAGGTGTTGTGATTGGTGGTGGTGATAAAAAATTGGTATTTTTTAAGAATTGGGGTAGGGTGTTTGATTTAGAGGATTTGTTAAGGGCTTCTGCTGAGGTATTAGGGAAAGGGACATTTGGGACATCTTACAAGGCAGTTATGGAGGCTGGTCCTGTGGCTGTGGCTGTGAAGAGGTTAAGGGATGTGACAATTTCTGAGAGGGAGTTTAAGGAGAAGATTGAGAAAGTTGGAACAATGGTTCATGAAAATTTGGCACCTCTTAGGGCTTATTATTATAGTAGAGATGAGAAGCTTCTTGTTCATGATTATTTGCATATGGGAAGTTTATCTGCACTTTTGCATGGAAACAGAGGAGGGGGTAGGACACCATTGACTTGGGAAATGAGATCAGGCATTGCACTTGGAGCCGCTCGCGGCATTGAATATCTACATTCACAAGGTCCTAATGTTTCTCATGGAAACATAAAGTCATCTAACATCCTACTAACCAAGTCCTACGATGCAAGAGTATCTGATTTCGGTCTTGCCCATCTTGTTGGTCCTTCATCCACACCCAATAGGGTTGCTGGCTACCGTGCTCCCGAGGTAACTGATCCTCGGAAAGTTTCTCAAAAGGCAGATGTATACAGCTTTGGTGTATTGCTCTTGGAACTTCTGACCGGAAAGGCTCCAACTCACGCCCTCTTGAACGACGAAGGTGTGGACCTTCCGAGATGGGTGCAATCGGTGGTTAAAGAAGAATGGACTTCCGAGGTCTTTGATCTTGAACTTCTTAGGTATCAAAATGTTGAAGAGGAAATGGTTCAGTTGTTGCAACTTGCCGTAGATTGTGCTGCTCCATACCCCGATAACCGCCCTTCCATGTCGCAAGTGAGACAGCACATAGAAGAGATGCATAGGTCTAGCTCGAAAGAGGGTAGTCAAGACCAAATTCAACAACCTGATGTGATCAGTGACATAGATGATATCTCTTCTAGATGA

Protein sequence

>Msa0960540

MKFYFSFFFFFLTLFLPSVKPDLTSERAALLTLRAAVTGRTLLWNTTSASPCNWAGVHCNQNHTHVVELHLPAVALSGNLPAGVFSELPNLHTLSLRFNSLSGPLPSDLSSCTSLKNLYLQQNLLSGELPATFFNLTGLVRLNLASNNFSGEIPVGFNNLTRLKTLYLQDNRFTGSLSEFESNPVQLAQFNVSNNMLNGSVPEKLQTFGKDSFLGNLLCGKPLNPCPKEGGANSGKGGANSGNGGRNSGNGGRNSSVVDDNNGFVRNKKKGKLSGGAIAGIVIGSVVILLLVVFALIVLCRNRNGEKIDEVARTLKHNQLIDEGIHGENVGHGNGNGNGYSSAAAMTVAAPMNGERSSEGVVIGGGDKKLVFFKNWGRVFDLEDLLRASAEVLGKGTFGTSYKAVMEAGPVAVAVKRLRDVTISEREFKEKIEKVGTMVHENLAPLRAYYYSRDEKLLVHDYLHMGSLSALLHGNRGGGRTPLTWEMRSGIALGAARGIEYLHSQGPNVSHGNIKSSNILLTKSYDARVSDFGLAHLVGPSSTPNRVAGYRAPEVTDPRKVSQKADVYSFGVLLLELLTGKAPTHALLNDEGVDLPRWVQSVVKEEWTSEVFDLELLRYQNVEEEMVQLLQLAVDCAAPYPDNRPSMSQVRQHIEEMHRSSSKEGSQDQIQQPDVISDIDDISSR*