AlfalfaGEDB | Msa0961390

M. sativa cultivar zhongmu-4 / Msa0961390

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961390	KEH16067.1	75.000	204	11	2	102	265	83	286	1.88e-98	300

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961390	A0A072TFB0	75.000	204	11	2	102	265	83	286	8.96e-99	300

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961390	MtrunA17_Chr6g0480541	79.255	188	2	1	115	265	1015	1202	3.18e-96	306
Msa0961390	MtrunA17_Chr6g0480541	93.750	64	4	0	54	117	363	426	5.26e-32	124
Msa0961390	MtrunA17_Chr6g0480541	87.234	47	5	1	1	47	239	284	1.05e-16	79.7
Msa0961390	MtrunA17_Chr5g0437131	50.532	188	56	1	115	265	520	707	1.48e-61	206
Msa0961390	MtrunA17_Chr6g0480291	92.308	78	5	1	188	265	1	77	2.11e-45	147
Msa0961390	MtrunA17_Chr6g0480471	96.078	51	2	0	163	213	25	75	6.74e-30	107
Msa0961390	MtrunA17_Chr6g0480481	97.959	49	1	0	115	163	115	163	6.62e-28	106
Msa0961390	MtrunA17_Chr6g0480331	92.683	41	3	0	210	250	2	42	3.13e-21	84.0
Msa0961390	MtrunA17_Chr6g0480191	97.143	35	1	0	231	265	1	35	3.65e-18	75.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961390	AT1G73450.1	69.149	188	21	1	115	265	958	1145	2.30e-85	276
Msa0961390	AT1G73450.1	65.517	58	20	0	54	111	322	379	1.78e-17	82.4
Msa0961390	AT1G73460.2	69.149	188	21	1	115	265	975	1162	3.85e-85	276
Msa0961390	AT1G73460.2	59.677	62	25	0	54	115	323	384	4.79e-17	81.3
Msa0961390	AT1G73460.1	69.149	188	21	1	115	265	975	1162	3.85e-85	276
Msa0961390	AT1G73460.1	59.677	62	25	0	54	115	323	384	4.79e-17	81.3
Msa0961390	AT3G17750.1	70.213	188	19	1	115	265	944	1131	3.43e-83	270
Msa0961390	AT3G17750.1	55.738	61	27	0	55	115	326	386	1.84e-13	70.5
Msa0961390	AT2G40120.1	47.059	187	62	1	115	264	378	564	4.56e-54	184
Msa0961390	AT4G03175.1	53.261	92	43	0	173	264	2	93	2.01e-32	115

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Find 59 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATATGCTTTATGAG+AGG	0.623112	6_3:-82987321	Msa0961390:CDS
AAAGTATGCTAGCAAAAGGA+CGG	0.592834	6_3:-82987366	Msa0961390:CDS
AAGAATCAAGGGAAATTGAT+CGG	0.562657	6_3:-82990704	Msa0961390:CDS
AAGATGTCTTCTTTGGAATC+AGG	0.354438	6_3:+82986867	None:intergenic
AAGTATGCTAGCAAAAGGAC+GGG	0.589744	6_3:-82987365	Msa0961390:CDS
AATACATTGTGACTTGAAGC+CGG	0.584972	6_3:-82988847	Msa0961390:CDS
ACATTACTTGCAAGGGTGAT+CGG	0.483894	6_3:-82987409	Msa0961390:CDS
ACCCTTGCAAGTAATGTTGC+CGG	0.412272	6_3:+82987415	None:intergenic
AGATTGGGGAAGCCAGATAT+TGG	0.390908	6_3:-82990577	Msa0961390:intron
AGCAGAAGGAAGAGTATCAA+AGG	0.545097	6_3:-82990430	Msa0961390:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAATCAAGGGAAATTGAT+CGG	-	chr6_3:82986755-82986774	Msa0961390:CDS	30.0%
TTCATCATTTAAAGAGAACG+TGG	-	chr6_3:82986798-82986817	Msa0961390:CDS	30.0%
CAAATTCATCTTCTGATGTA+TGG	-	chr6_3:82986857-82986876	Msa0961390:CDS	30.0%
TTCTGATGTATGGAAAGATT+GGG	-	chr6_3:82986867-82986886	Msa0961390:CDS	30.0%
AGATCTGAACAAATAACATC+GGG	-	chr6_3:82986978-82986997	Msa0961390:intron	30.0%
GGACAAGAAATTAAAAATGC+AGG	-	chr6_3:82987193-82987212	Msa0961390:intron	30.0%
CTTTCATCTCTTGTAATTGA+TGG	-	chr6_3:82987711-82987730	Msa0961390:intron	30.0%
GTTCCAAATTGGAATAGAAT+TGG	-	chr6_3:82987755-82987774	Msa0961390:intron	30.0%
AAATGAAAACTAACATCCTG+AGG	+	chr6_3:82988118-82988137	None:intergenic	30.0%
TTCAAACTTAATTTCCGTGA+AGG	+	chr6_3:82988214-82988233	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	82986745	82990736	82986745	ID=Msa0961390;Name=Msa0961390
chr6_3	mRNA	82986745	82990736	82986745	ID=Msa0961390-mRNA-1;Parent=Msa0961390;Name=Msa0961390-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0\|0.25\|0\|0.6\|0.5\|0.8\|5\|0\|265
chr6_3	exon	82986745	82986909	82986745	ID=Msa0961390-mRNA-1:exon:14843;Parent=Msa0961390-mRNA-1
chr6_3	exon	82987315	82987458	82987315	ID=Msa0961390-mRNA-1:exon:14842;Parent=Msa0961390-mRNA-1
chr6_3	exon	82988755	82988897	82988755	ID=Msa0961390-mRNA-1:exon:14841;Parent=Msa0961390-mRNA-1
chr6_3	exon	82990267	82990450	82990267	ID=Msa0961390-mRNA-1:exon:14840;Parent=Msa0961390-mRNA-1
chr6_3	exon	82990578	82990736	82990578	ID=Msa0961390-mRNA-1:exon:14839;Parent=Msa0961390-mRNA-1
chr6_3	CDS	82990578	82990736	82990578	ID=Msa0961390-mRNA-1:cds;Parent=Msa0961390-mRNA-1
chr6_3	CDS	82990267	82990450	82990267	ID=Msa0961390-mRNA-1:cds;Parent=Msa0961390-mRNA-1
chr6_3	CDS	82988755	82988897	82988755	ID=Msa0961390-mRNA-1:cds;Parent=Msa0961390-mRNA-1
chr6_3	CDS	82987315	82987458	82987315	ID=Msa0961390-mRNA-1:cds;Parent=Msa0961390-mRNA-1
chr6_3	CDS	82986745	82986909	82986745	ID=Msa0961390-mRNA-1:cds;Parent=Msa0961390-mRNA-1

Gene Sequence

>Msa0961390

ATGCAAAACAAAGAATCAAGGGAAATTGATCGGCAGCAGCTTAAATTTTGTAGTTCATCATTTAAAGAGAACGTGGCTGATAACGTGTTGTCAAGAGCTGCTAATGAGAATGCAAATTCATCTTCTGATGTATGGAAAGATTGGGGAAGCCAGATATTGCAGAAGGAAGAGTATCAAAGGCTTCCTCCTGTGAAAATTAAGTCTGAAGATAAGCCGTTGACTATTAATTGGAGCGAGAAGTTTGAATCCGATGGATTGGCTGCTAAGCTGGCAAGTGCTGACAGCTCTTTACTTATTGGATCATATCTGGATGTACCTATTGGACAAGAAATTAAAAATGCAGCACTTCAATATTTACATAACCTTGGACTAATACATTGTGACTTGAAGCCGGAGAATATTTTGGTTAAAAGCTATAGCCGATGTGAGGTGAAGGTCGTTGATCTTGGAAGTAGTTGTTTTGAGACAGATCACCTTTGCTCATATGTCTTATTTCAAAATGATTCCCCGGCAACATTACTTGCAAGGGTGATCGGGATTATCGGTCCAATTGGTCAAAGTATGCTAGCAAAAGGACGGGATACCTATAAGTACTTCACAAAAAATCATATGCTTTATGAGAGGAATCAGGAAAGCAACAGGTTAGAATACCTGATTCCAAAGAAGACATCTTTGAGGCATAGATTACCGATGGGGGACCAAGGCTTCATTGACTTTGTTGCTCATTTGCTCGAGGTTAACCCGAAGAAGCGACCTTCTGCATCTGAAGCTCTAAAGCACCCCTGGTTATCATAC

Protein sequence

>Msa0961390

MQNKESREIDRQQLKFCSSSFKENVADNVLSRAANENANSSSDVWKDWGSQILQKEEYQRLPPVKIKSEDKPLTINWSEKFESDGLAAKLASADSSLLIGSYLDVPIGQEIKNAALQYLHNLGLIHCDLKPENILVKSYSRCEVKVVDLGSSCFETDHLCSYVLFQNDSPATLLARVIGIIGPIGQSMLAKGRDTYKYFTKNHMLYERNQESNRLEYLIPKKTSLRHRLPMGDQGFIDFVAHLLEVNPKKRPSASEALKHPWLSY