AlfalfaGEDB | Msa0963710

M. sativa cultivar zhongmu-4 / Msa0963710

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963710	sp\|Q93YN1\|CRPK1_ARATH	53.378	296	124	3	31	312	26	321	3.48e-105	316

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963710	A0A2K3MPD5	60.405	346	113	4	1	326	1	342	3.10e-137	404

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963710	MtrunA17_Chr8g0358911	60.116	346	114	4	1	326	1	342	9.16e-140	400
Msa0963710	MtrunA17_Chr2g0312561	54.103	329	136	4	1	317	1	326	3.50e-120	350
Msa0963710	MtrunA17_Chr2g0312571	54.819	332	134	5	1	317	1	331	7.25e-119	350
Msa0963710	MtrunA17_Chr8g0358901	49.107	336	123	3	31	322	14	345	2.36e-99	296
Msa0963710	MtrunA17_Chr8g0360591	51.701	294	130	2	31	312	29	322	1.15e-96	291
Msa0963710	MtrunA17_Chr2g0281971	39.365	315	175	4	10	312	16	326	1.88e-77	241
Msa0963710	MtrunA17_Chr2g0314961	42.357	314	149	5	3	307	119	409	6.83e-77	242
Msa0963710	MtrunA17_Chr2g0315081	42.368	321	167	4	3	311	617	931	7.33e-77	253
Msa0963710	MtrunA17_Chr2g0315061	42.759	290	154	2	30	307	21	310	1.07e-76	239
Msa0963710	MtrunA17_Chr5g0438071	42.284	324	167	5	3	313	623	939	1.08e-75	250

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963710	AT1G16670.1	53.378	296	124	3	31	312	26	321	3.54e-106	316
Msa0963710	AT3G09010.1	39.935	308	169	5	24	317	24	329	3.58e-76	239
Msa0963710	AT1G53420.1	43.548	310	159	3	11	308	593	898	1.49e-75	249
Msa0963710	AT1G53430.2	45.424	295	147	3	30	311	612	905	3.97e-75	249
Msa0963710	AT1G53430.1	45.424	295	147	3	30	311	653	946	6.47e-75	249
Msa0963710	AT1G53440.1	44.558	294	151	2	30	311	651	944	1.19e-74	248
Msa0963710	AT3G14840.2	40.446	314	171	3	11	312	641	950	7.90e-74	246
Msa0963710	AT1G56130.3	43.493	292	148	5	33	311	482	769	9.82e-73	240
Msa0963710	AT1G56120.3	43.092	304	154	6	24	312	520	819	1.75e-72	240
Msa0963710	AT1G56130.1	43.493	292	148	5	33	311	533	820	2.05e-72	240

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Find 62 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGATGTTTACAGTTT+TGG	0.218688	6_3:+88820394	Msa0963710:CDS
AAGACCGAAATTTGAAATCA+CGG	0.541130	6_3:-88820206	None:intergenic
AATAGGGAGTACTAAAAGGT+GGG	0.590822	6_3:+88819479	Msa0963710:intron
AATGTAATATCTACTTTGAT+TGG	0.353910	6_3:+88820056	Msa0963710:CDS
AATTTCCATCAGTACAGAGA+AGG	0.556745	6_3:-88820690	None:intergenic
AATTTGAAATCACGGGTGTA+AGG	0.500761	6_3:-88820198	None:intergenic
ACACCCGTGATTTCAAATTT+CGG	0.389135	6_3:+88820202	Msa0963710:CDS
ACACGATGTAGGATATGTAT+TGG	0.482662	6_3:+88820082	Msa0963710:CDS
ACATGTCATTTGTCCCATTG+TGG	0.548326	6_3:-88820371	None:intergenic
ACGGGAACTTTATAAGAAAA+AGG	0.325369	6_3:+88820591	Msa0963710:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCGTCATATCTAATGTAGAT+AGG	-	chr6_3:88819143-88819162	None:intergenic	30.0%
ATTAGCAGAACTGAAATTGT+TGG	-	chr6_3:88819290-88819309	None:intergenic	30.0%
TTCAGTTCTGCTAATCATAT+CGG	+	chr6_3:88819295-88819314	Msa0963710:CDS	30.0%
TGTTAAGGTTCCATTAATAC+AGG	-	chr6_3:88819441-88819460	None:intergenic	30.0%
TATAGTGCATTTACCTAGAA+GGG	-	chr6_3:88819689-88819708	None:intergenic	30.0%
GTTACATACCTGATGTAATA+AGG	-	chr6_3:88820256-88820275	None:intergenic	30.0%
TTTATTTCAGCTGTCATGAT+GGG	+	chr6_3:88820329-88820348	Msa0963710:intron	30.0%
GTCATCCTTATAGAAATAGT+CGG	+	chr6_3:88820418-88820437	Msa0963710:CDS	30.0%
ATAAGCAATACGATGAACTA+TGG	-	chr6_3:88820546-88820565	None:intergenic	30.0%
TATGTTCTTATTACAGACAC+GGG	+	chr6_3:88820573-88820592	Msa0963710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	88819061	88821258	88819061	ID=Msa0963710;Name=Msa0963710
chr6_3	mRNA	88819061	88821258	88819061	ID=Msa0963710-mRNA-1;Parent=Msa0963710;Name=Msa0963710-mRNA-1;_AED=0.18;_eAED=0.18;_QI=2\|0.16\|0\|0.85\|0.83\|0.85\|7\|0\|328
chr6_3	exon	88819061	88819135	88819061	ID=Msa0963710-mRNA-1:exon:16096;Parent=Msa0963710-mRNA-1
chr6_3	exon	88819233	88819345	88819233	ID=Msa0963710-mRNA-1:exon:16097;Parent=Msa0963710-mRNA-1
chr6_3	exon	88819484	88819694	88819484	ID=Msa0963710-mRNA-1:exon:16098;Parent=Msa0963710-mRNA-1
chr6_3	exon	88820047	88820266	88820047	ID=Msa0963710-mRNA-1:exon:16099;Parent=Msa0963710-mRNA-1
chr6_3	exon	88820339	88820471	88820339	ID=Msa0963710-mRNA-1:exon:16100;Parent=Msa0963710-mRNA-1
chr6_3	exon	88820589	88820778	88820589	ID=Msa0963710-mRNA-1:exon:16101;Parent=Msa0963710-mRNA-1
chr6_3	exon	88821215	88821258	88821215	ID=Msa0963710-mRNA-1:exon:16102;Parent=Msa0963710-mRNA-1
chr6_3	five_prime_UTR	88819061	88819062	88819061	ID=Msa0963710-mRNA-1:five_prime_utr;Parent=Msa0963710-mRNA-1
chr6_3	CDS	88819063	88819135	88819063	ID=Msa0963710-mRNA-1:cds;Parent=Msa0963710-mRNA-1
chr6_3	CDS	88819233	88819345	88819233	ID=Msa0963710-mRNA-1:cds;Parent=Msa0963710-mRNA-1
chr6_3	CDS	88819484	88819694	88819484	ID=Msa0963710-mRNA-1:cds;Parent=Msa0963710-mRNA-1
chr6_3	CDS	88820047	88820266	88820047	ID=Msa0963710-mRNA-1:cds;Parent=Msa0963710-mRNA-1
chr6_3	CDS	88820339	88820471	88820339	ID=Msa0963710-mRNA-1:cds;Parent=Msa0963710-mRNA-1
chr6_3	CDS	88820589	88820778	88820589	ID=Msa0963710-mRNA-1:cds;Parent=Msa0963710-mRNA-1
chr6_3	CDS	88821215	88821258	88821215	ID=Msa0963710-mRNA-1:cds;Parent=Msa0963710-mRNA-1

Gene Sequence

>Msa0963710

ATGTCTTGGTTTAATTTCTTATTTGGTAAGAAACCATCTTCTAATGTAAGCCGTGATCCTAAAATTCTTGAAGGACTTTCAAGCACTCAGATAAAAGTTTATACCTACGAAGAACTAAAAAGAGCCTCCAACAATTTCAGTTCTGCTAATCATATCGGGGGTGGTAATTTTGGTTCTGTCTATAAGGGAGTACTAAAAGGTGGGAACTTAATGGCTCTAAAAGCTTTTTTAGTTCCAACAAGAGAGGGGTTGAATGAATTCTTGACCGATGTTAATGCTATTTTCAAAATAAAACATGAAAATTTGGTTGAACTATATGGTTACTGTGCAGAAGGAGATCACCGAATTTTGGTCTACAGATACCTTGAGAACAAGAGCCTTGCAAAAACCCTTCTAGGTGGAGGTCAATGTAATATCTACTTTGATTGGCGAACACGATGTAGGATATGTATTGGAGTTGCACGTGGACTTGCCTTTCTTCATGAGCAAGAAAGACCAAACAATATTCACAGCGAGCTAACATGCAACAATATTCTACTTGACAAAGACCTTACACCCGTGATTTCAAATTTCGGTCTTGGAAGACTTATTCGAGGAGACCGCCCTTATTACATCAGCTGTCATGATGGGCATGTTGCACCAGAGTACGCCACAATGGGACAAATGACATGTAAAACAGATGTTTACAGTTTTGGGGTCATCCTTATAGAAATAGTCGGAGGGAGATCTAACACATATCTTATACGACAAACACGGGAACTTTATAAGAAAAAGGAGCTATTCAAGTTGGTAGATTTGTCTCTAAATGGGTTTTTCGATGGTGAGGAGGCTTATAAGATTCTTAAGATTGCCCTTCTCTGTACTGATGGAAATTTTAAGAGGAGGCCAACTATGTCTTCTGTGGTCAAAATGCTCACCGGAGAAATTGATGTTAACAAAGGTTTTGCAACGATCACTAACTCTGGTGATTTCAAATTGTTCAGA

Protein sequence

>Msa0963710

MSWFNFLFGKKPSSNVSRDPKILEGLSSTQIKVYTYEELKRASNNFSSANHIGGGNFGSVYKGVLKGGNLMALKAFLVPTREGLNEFLTDVNAIFKIKHENLVELYGYCAEGDHRILVYRYLENKSLAKTLLGGGQCNIYFDWRTRCRICIGVARGLAFLHEQERPNNIHSELTCNNILLDKDLTPVISNFGLGRLIRGDRPYYISCHDGHVAPEYATMGQMTCKTDVYSFGVILIEIVGGRSNTYLIRQTRELYKKKELFKLVDLSLNGFFDGEEAYKILKIALLCTDGNFKRRPTMSSVVKMLTGEIDVNKGFATITNSGDFKLFR