AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0964080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0964080 MtrunA17_Chr8g0339041 77.419 124 28 0 47 170 139 262 5.38e-64 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0964080 AT1G79220.1 46.094 128 68 1 44 170 162 289 3.03e-32 119

Find 37 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCCTAGTGGATCAGGTTT+TGG 0.256158 6_3:-90238506 Msa0964080:CDS
CCTTCGTTGCTATGTGAAAA+TGG 0.268635 6_3:-90237195 Msa0964080:CDS
TCTTACAAAGTTTGGTTTAT+CGG 0.270206 6_3:-90236941 Msa0964080:CDS
AAATATGTGGTGACGTTAAT+AGG 0.278279 6_3:-90237003 Msa0964080:CDS
GTTGACAATGTCATAATATT+AGG 0.285517 6_3:+90236886 None:intergenic
GAAAATGGCTATGACATTAA+AGG 0.301086 6_3:-90237180 Msa0964080:CDS
TATGACATTGTCAACTGATA+TGG 0.327135 6_3:-90236878 Msa0964080:CDS
TTTGATACTGCACTTGTGAT+TGG 0.335068 6_3:-90238527 Msa0964080:CDS
GTGTTAAATCTTACAAAGTT+TGG 0.362500 6_3:-90236949 Msa0964080:CDS
GTGATTGGCCCTAGTGGATC+AGG 0.372420 6_3:-90238512 Msa0964080:CDS
GTTGCACAATATGAAGAGTT+AGG 0.395236 6_3:-90237153 Msa0964080:CDS
AAGAGTTAGGCGTATTGAAA+AGG 0.444866 6_3:-90237140 Msa0964080:CDS
TCTCCTTCGCATGGAACACC+TGG 0.469405 6_3:+90238449 None:intergenic
TTCCTTTGATGATGAGAAGA+TGG 0.470731 6_3:-90237070 Msa0964080:CDS
ATGGTATATATAAGCAGGAC+TGG 0.475180 6_3:-90237051 Msa0964080:CDS
ATTACTCAAGGAGGAGGTCC+AGG 0.491187 6_3:-90238467 Msa0964080:CDS
AGGCGTATTGAAAAGGGACT+TGG 0.493691 6_3:-90237133 Msa0964080:CDS
TTATCGGATGATGAGATAGT+TGG 0.498981 6_3:-90236925 Msa0964080:CDS
TGCAGCCGGCGATTATTACA+AGG 0.503215 6_3:-90237095 Msa0964080:CDS
AGAAGATGGTATATATAAGC+AGG 0.519672 6_3:-90237056 Msa0964080:CDS
CAACGAAGGGTTTCTTGCAA+TGG 0.530280 6_3:+90237209 None:intergenic
AGAGTTAGGCGTATTGAAAA+GGG 0.532449 6_3:-90237139 Msa0964080:CDS
TGGTATATATAAGCAGGACT+GGG 0.532776 6_3:-90237050 Msa0964080:CDS
GCACTTGTGATTGGCCCTAG+TGG 0.544194 6_3:-90238518 Msa0964080:CDS
AGTCCAAAACCTGATCCACT+AGG 0.566504 6_3:+90238503 None:intergenic
TGCAAAATTGTACAAATATG+TGG 0.567808 6_3:-90237016 Msa0964080:CDS
GAAGTCCTTGTAATAATCGC+CGG 0.570186 6_3:+90237090 None:intergenic
TACCATCTTCTCATCATCAA+AGG 0.572098 6_3:+90237068 None:intergenic
CTAATAGACTCTCCTTCGCA+TGG 0.580381 6_3:+90238440 None:intergenic
CAAGATGGAAAGATTACTCA+AGG 0.582815 6_3:-90238479 Msa0964080:CDS
GGAAAGATTACTCAAGGAGG+AGG 0.596488 6_3:-90238473 Msa0964080:CDS
CGTGGAAATGATTCACGCAA+AGG 0.597282 6_3:-90236971 Msa0964080:CDS
GGTCCAGGTGTTCCATGCGA+AGG 0.614059 6_3:-90238452 Msa0964080:CDS
GTCCAAAACCTGATCCACTA+GGG 0.614268 6_3:+90238504 None:intergenic
GTTAATAGGAATCTCTCGCG+TGG 0.661196 6_3:-90236989 Msa0964080:CDS
GGTATATATAAGCAGGACTG+GGG 0.703023 6_3:-90237049 Msa0964080:CDS
GATGGAAAGATTACTCAAGG+AGG 0.704189 6_3:-90238476 Msa0964080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGTTAACTTTTAGTTAGA+AGG - chr6_3:90237031-90237050 Msa0964080:CDS 20.0%
!! TAATTAAACAAGTTAGTTAG+AGG - chr6_3:90237071-90237090 Msa0964080:CDS 20.0%
!! CAATTTCATAAGATTCAATA+TGG - chr6_3:90237207-90237226 Msa0964080:CDS 20.0%
!! TAGAAATTACATAAATCTGA+TGG - chr6_3:90237497-90237516 Msa0964080:intron 20.0%
!! ACAATAAGTCAATAACAATT+GGG - chr6_3:90237746-90237765 Msa0964080:intron 20.0%
! AAATTCATTGTACAATGAGT+AGG + chr6_3:90237164-90237183 None:intergenic 25.0%
!! TGAATTTTCAATCCAATCAA+TGG - chr6_3:90237177-90237196 Msa0964080:CDS 25.0%
!! GAATTTTCAATCCAATCAAT+GGG - chr6_3:90237178-90237197 Msa0964080:CDS 25.0%
!! ATTGACTTATTGTGAGATAT+AGG + chr6_3:90237739-90237758 None:intergenic 25.0%
! CACAATAAGTCAATAACAAT+TGG - chr6_3:90237745-90237764 Msa0964080:intron 25.0%
! TTGTGAAGATTATCAATTGT+AGG - chr6_3:90238078-90238097 Msa0964080:intron 25.0%
! TGCAAAATTGTACAAATATG+TGG - chr6_3:90238390-90238409 Msa0964080:intron 25.0%
! GTGTTAAATCTTACAAAGTT+TGG - chr6_3:90238457-90238476 Msa0964080:CDS 25.0%
!! TCTTACAAAGTTTGGTTTAT+CGG - chr6_3:90238465-90238484 Msa0964080:CDS 25.0%
! GTTGACAATGTCATAATATT+AGG + chr6_3:90238523-90238542 None:intergenic 25.0%
GTCTATTAGCGAAAAAATTG+CGG - chr6_3:90236980-90236999 Msa0964080:CDS 30.0%
ATCCTGCATGTTATATAAAC+TGG - chr6_3:90237136-90237155 Msa0964080:CDS 30.0%
ATCCAGTTTATATAACATGC+AGG + chr6_3:90237141-90237160 None:intergenic 30.0%
TCAGTTATATGGTTAACTCT+CGG + chr6_3:90237293-90237312 None:intergenic 30.0%
AGACTATGTACTCAGTTATA+TGG + chr6_3:90237304-90237323 None:intergenic 30.0%
TCCTATCTAGACTTACAAAA+CGG + chr6_3:90237333-90237352 None:intergenic 30.0%
ACGACATTATGTAACTTTGT+GGG - chr6_3:90237455-90237474 Msa0964080:intron 30.0%
AGCTTCAATAATTGCAACAA+AGG + chr6_3:90237840-90237859 None:intergenic 30.0%
ATTCAACCTGATATTTGGTT+GGG + chr6_3:90237903-90237922 None:intergenic 30.0%
AATTCAACCTGATATTTGGT+TGG + chr6_3:90237904-90237923 None:intergenic 30.0%
!!! TACGGAAGTTTTGAGTAAAT+GGG - chr6_3:90237930-90237949 Msa0964080:intron 30.0%
! TTTTACGCAAACAATGACAT+AGG + chr6_3:90238160-90238179 None:intergenic 30.0%
TATGTCATTGTTTGCGTAAA+AGG - chr6_3:90238159-90238178 Msa0964080:intron 30.0%
GAAAATGGCTATGACATTAA+AGG - chr6_3:90238226-90238245 Msa0964080:intron 30.0%
AGAAGATGGTATATATAAGC+AGG - chr6_3:90238350-90238369 Msa0964080:intron 30.0%
! AAATATGTGGTGACGTTAAT+AGG - chr6_3:90238403-90238422 Msa0964080:intron 30.0%
TATGACATTGTCAACTGATA+TGG - chr6_3:90238528-90238547 Msa0964080:CDS 30.0%
TTTGATACTGCACTTGTGAT+TGG - chr6_3:90236879-90236898 Msa0964080:CDS 35.0%
CAAGATGGAAAGATTACTCA+AGG - chr6_3:90236927-90236946 Msa0964080:CDS 35.0%
TGCAGGATCAATCGAATAAA+GGG + chr6_3:90237124-90237143 None:intergenic 35.0%
ATGCAGGATCAATCGAATAA+AGG + chr6_3:90237125-90237144 None:intergenic 35.0%
AATTGATCTCACCCATTGAT+TGG + chr6_3:90237192-90237211 None:intergenic 35.0%
! TCCGTTTTGTAAGTCTAGAT+AGG - chr6_3:90237329-90237348 Msa0964080:intron 35.0%
GACGACATTATGTAACTTTG+TGG - chr6_3:90237454-90237473 Msa0964080:intron 35.0%
CAATTATTGAAGCTCCTTGT+CGG - chr6_3:90237846-90237865 Msa0964080:intron 35.0%
TGGCAATTCAACCTGATATT+TGG + chr6_3:90237908-90237927 None:intergenic 35.0%
CATTTACTCAAAACTTCCGT+AGG + chr6_3:90237931-90237950 None:intergenic 35.0%
!!! CTACGGAAGTTTTGAGTAAA+TGG - chr6_3:90237929-90237948 Msa0964080:intron 35.0%
!!! ACGGAAGTTTTGAGTAAATG+GGG - chr6_3:90237931-90237950 Msa0964080:intron 35.0%
!! TTTTGAGTAAATGGGGTTTG+TGG - chr6_3:90237938-90237957 Msa0964080:intron 35.0%
ACTTTGATTGTTCAGTGTCT+TGG - chr6_3:90238037-90238056 Msa0964080:intron 35.0%
CTTTGATTGTTCAGTGTCTT+GGG - chr6_3:90238038-90238057 Msa0964080:intron 35.0%
! CATTTTCACATAGCAACGAA+GGG + chr6_3:90238213-90238232 None:intergenic 35.0%
GTTGCACAATATGAAGAGTT+AGG - chr6_3:90238253-90238272 Msa0964080:intron 35.0%
AAGAGTTAGGCGTATTGAAA+AGG - chr6_3:90238266-90238285 Msa0964080:intron 35.0%
AGAGTTAGGCGTATTGAAAA+GGG - chr6_3:90238267-90238286 Msa0964080:intron 35.0%
!! TTCCTTTGATGATGAGAAGA+TGG - chr6_3:90238336-90238355 Msa0964080:intron 35.0%
TACCATCTTCTCATCATCAA+AGG + chr6_3:90238341-90238360 None:intergenic 35.0%
ATGGTATATATAAGCAGGAC+TGG - chr6_3:90238355-90238374 Msa0964080:intron 35.0%
TGGTATATATAAGCAGGACT+GGG - chr6_3:90238356-90238375 Msa0964080:intron 35.0%
TTATCGGATGATGAGATAGT+TGG - chr6_3:90238481-90238500 Msa0964080:CDS 35.0%
!!! GATGAGATAGTTGGTCTTTT+TGG - chr6_3:90238490-90238509 Msa0964080:CDS 35.0%
GATGGAAAGATTACTCAAGG+AGG - chr6_3:90236930-90236949 Msa0964080:CDS 40.0%
CAGGATCAATCGAATAAAGG+GGG + chr6_3:90237122-90237141 None:intergenic 40.0%
GCAGGATCAATCGAATAAAG+GGG + chr6_3:90237123-90237142 None:intergenic 40.0%
GTGAAGATCTGAAAAAGCAG+TGG + chr6_3:90237249-90237268 None:intergenic 40.0%
ATGGTTAACTCTCGGTTAGA+AGG + chr6_3:90237285-90237304 None:intergenic 40.0%
GTAAGTCTAGATAGGAGAGT+TGG - chr6_3:90237337-90237356 Msa0964080:intron 40.0%
ATAGGAGAGTTGGAGTATGA+GGG - chr6_3:90237347-90237366 Msa0964080:intron 40.0%
!!! TGGATAGTTTTGAGTCTGCT+CGG - chr6_3:90237374-90237393 Msa0964080:intron 40.0%
AACTTTGTGGGACAGTACAT+AGG - chr6_3:90237467-90237486 Msa0964080:intron 40.0%
ACTTTGTGGGACAGTACATA+GGG - chr6_3:90237468-90237487 Msa0964080:intron 40.0%
GATGCTCGAGAAATGAAACA+TGG + chr6_3:90237569-90237588 None:intergenic 40.0%
AGGCTTGTAGTTCAGATCTT+TGG + chr6_3:90237660-90237679 None:intergenic 40.0%
AGGATCAAACAGAACATTGC+AGG + chr6_3:90237680-90237699 None:intergenic 40.0%
AAGAAGCCAACGAAGGAAAA+GGG + chr6_3:90237785-90237804 None:intergenic 40.0%
CAAAATGACAGTCTCTTCGA+AGG + chr6_3:90237815-90237834 None:intergenic 40.0%
TTACTCAAAACTTCCGTAGG+TGG + chr6_3:90237928-90237947 None:intergenic 40.0%
!! GCACAGTGTTGATCTTTGTA+AGG - chr6_3:90238004-90238023 Msa0964080:intron 40.0%
TGTTCAGTGTCTTGGGTTTA+AGG - chr6_3:90238045-90238064 Msa0964080:intron 40.0%
GTCGAATTCAGGGTTAATCT+GGG + chr6_3:90238122-90238141 None:intergenic 40.0%
! CCATTTTCACATAGCAACGA+AGG + chr6_3:90238214-90238233 None:intergenic 40.0%
CCTTCGTTGCTATGTGAAAA+TGG - chr6_3:90238211-90238230 Msa0964080:intron 40.0%
!!! TCAAATGCTGATTTTGCAGC+CGG - chr6_3:90238297-90238316 Msa0964080:intron 40.0%
GAAGTCCTTGTAATAATCGC+CGG + chr6_3:90238319-90238338 None:intergenic 40.0%
GGTATATATAAGCAGGACTG+GGG - chr6_3:90238357-90238376 Msa0964080:intron 40.0%
GTCCAAAACCTGATCCACTA+GGG + chr6_3:90236905-90236924 None:intergenic 45.0%
AGTCCAAAACCTGATCCACT+AGG + chr6_3:90236906-90236925 None:intergenic 45.0%
!!! TCAGGTTTTGGACTGCAAGA+TGG - chr6_3:90236912-90236931 Msa0964080:CDS 45.0%
GGAAAGATTACTCAAGGAGG+AGG - chr6_3:90236933-90236952 Msa0964080:CDS 45.0%
CTAATAGACTCTCCTTCGCA+TGG + chr6_3:90236969-90236988 None:intergenic 45.0%
GATAGGAGAGTTGGAGTATG+AGG - chr6_3:90237346-90237365 Msa0964080:intron 45.0%
TAGGAGAGTTGGAGTATGAG+GGG - chr6_3:90237348-90237367 Msa0964080:intron 45.0%
! CATTTCTCGAGCATCGTTGA+CGG - chr6_3:90237574-90237593 Msa0964080:intron 45.0%
TGCAAGTGGTGTTTGTGAAG+AGG + chr6_3:90237700-90237719 None:intergenic 45.0%
AGAAGCCAACGAAGGAAAAG+GGG + chr6_3:90237784-90237803 None:intergenic 45.0%
CAAGAAGCCAACGAAGGAAA+AGG + chr6_3:90237786-90237805 None:intergenic 45.0%
!!! CTTTTCCTTCGTTGGCTTCT+TGG - chr6_3:90237784-90237803 Msa0964080:intron 45.0%
TCAAACCAAGAAGCCAACGA+AGG + chr6_3:90237792-90237811 None:intergenic 45.0%
GGAAGTCCCAACCAAATATC+AGG - chr6_3:90237894-90237913 Msa0964080:intron 45.0%
!! TCAGGTTGAATTGCCACCTA+CGG - chr6_3:90237912-90237931 Msa0964080:intron 45.0%
CGTCGAATTCAGGGTTAATC+TGG + chr6_3:90238123-90238142 None:intergenic 45.0%
TTAACCCTGAATTCGACGAG+AGG - chr6_3:90238125-90238144 Msa0964080:intron 45.0%
CAACGAAGGGTTTCTTGCAA+TGG + chr6_3:90238200-90238219 None:intergenic 45.0%
AGGCGTATTGAAAAGGGACT+TGG - chr6_3:90238273-90238292 Msa0964080:intron 45.0%
GTTAATAGGAATCTCTCGCG+TGG - chr6_3:90238417-90238436 Msa0964080:intron 45.0%
CGTGGAAATGATTCACGCAA+AGG - chr6_3:90238435-90238454 Msa0964080:CDS 45.0%
ATTACTCAAGGAGGAGGTCC+AGG - chr6_3:90236939-90236958 Msa0964080:CDS 50.0%
AGTTGGAGTATGAGGGGACA+TGG - chr6_3:90237354-90237373 Msa0964080:intron 50.0%
CATGGTTGTTCTGCGTTGTG+AGG + chr6_3:90237551-90237570 None:intergenic 50.0%
AGAGACTTCAGCTGTGCAAG+TGG + chr6_3:90237714-90237733 None:intergenic 50.0%
! AAACCCCCCTTTTCCTTCGT+TGG - chr6_3:90237776-90237795 Msa0964080:intron 50.0%
AAGCCAACGAAGGAAAAGGG+GGG + chr6_3:90237782-90237801 None:intergenic 50.0%
GAAGCCAACGAAGGAAAAGG+GGG + chr6_3:90237783-90237802 None:intergenic 50.0%
CACTCCTGCCACCTATTTGA+CGG - chr6_3:90237873-90237892 Msa0964080:intron 50.0%
ACTTCCGTCAAATAGGTGGC+AGG + chr6_3:90237880-90237899 None:intergenic 50.0%
TGGGACTTCCGTCAAATAGG+TGG + chr6_3:90237884-90237903 None:intergenic 50.0%
GGTTGGGACTTCCGTCAAAT+AGG + chr6_3:90237887-90237906 None:intergenic 50.0%
!! ATTTGACGAGAGTGTTCGCC+CGG - chr6_3:90237970-90237989 Msa0964080:intron 50.0%
ACTGTGCAGTGAAGTTCACC+GGG + chr6_3:90237991-90238010 None:intergenic 50.0%
CACTGTGCAGTGAAGTTCAC+CGG + chr6_3:90237992-90238011 None:intergenic 50.0%
CAAGCCTCTCGTCGAATTCA+GGG + chr6_3:90238132-90238151 None:intergenic 50.0%
TCAAGCCTCTCGTCGAATTC+AGG + chr6_3:90238133-90238152 None:intergenic 50.0%
TGCAGCCGGCGATTATTACA+AGG - chr6_3:90238311-90238330 Msa0964080:intron 50.0%
!! AAGCAGGACTGGGGTTTTGA+AGG - chr6_3:90238366-90238385 Msa0964080:intron 50.0%
! GCACTTGTGATTGGCCCTAG+TGG - chr6_3:90236888-90236907 Msa0964080:CDS 55.0%
! GTGATTGGCCCTAGTGGATC+AGG - chr6_3:90236894-90236913 Msa0964080:CDS 55.0%
!! GGCCCTAGTGGATCAGGTTT+TGG - chr6_3:90236900-90236919 Msa0964080:CDS 55.0%
TCTCCTTCGCATGGAACACC+TGG + chr6_3:90236960-90236979 None:intergenic 55.0%
TCTGCTCGGTATCAGAGCTG+CGG - chr6_3:90237388-90237407 Msa0964080:intron 55.0%
GGTATCAGAGCTGCGGCATT+GGG - chr6_3:90237395-90237414 Msa0964080:intron 55.0%
CGTCAAATAGGTGGCAGGAG+TGG + chr6_3:90237875-90237894 None:intergenic 55.0%
GGTCCAGGTGTTCCATGCGA+AGG - chr6_3:90236954-90236973 Msa0964080:CDS 60.0%
CGGTATCAGAGCTGCGGCAT+TGG - chr6_3:90237394-90237413 Msa0964080:intron 60.0%
! AGCTGCGGCATTGGGATGTG+TGG - chr6_3:90237403-90237422 Msa0964080:intron 60.0%
! GCTGCGGCATTGGGATGTGT+GGG - chr6_3:90237404-90237423 Msa0964080:intron 60.0%
GGCAGGAGTGGAAACCGACA+AGG + chr6_3:90237863-90237882 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 90236870 90238558 90236870 ID=Msa0964080;Name=Msa0964080
chr6_3 mRNA 90236870 90238558 90236870 ID=Msa0964080-mRNA-1;Parent=Msa0964080;Name=Msa0964080-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|0|0|0.5|1|1|2|0|170
chr6_3 exon 90238424 90238558 90238424 ID=Msa0964080-mRNA-1:exon:16234;Parent=Msa0964080-mRNA-1
chr6_3 exon 90236870 90237247 90236870 ID=Msa0964080-mRNA-1:exon:16233;Parent=Msa0964080-mRNA-1
chr6_3 CDS 90238424 90238558 90238424 ID=Msa0964080-mRNA-1:cds;Parent=Msa0964080-mRNA-1
chr6_3 CDS 90236870 90237247 90236870 ID=Msa0964080-mRNA-1:cds;Parent=Msa0964080-mRNA-1
Gene Sequence

>Msa0964080

ATGATTTTCTTTGATACTGCACTTGTGATTGGCCCTAGTGGATCAGGTTTTGGACTGCAAGATGGAAAGATTACTCAAGGAGGAGGTCCAGGTGTTCCATGCGAAGGAGAGTCTATTAGCGAAAAAATTGCGGCAGAAATGCTTCATAAGACCATTGCAAGAAACCCTTCGTTGCTATGTGAAAATGGCTATGACATTAAAGGTATTGTTGCACAATATGAAGAGTTAGGCGTATTGAAAAGGGACTTGGTTCAAATGCTGATTTTGCAGCCGGCGATTATTACAAGGACTTCCTTTGATGATGAGAAGATGGTATATATAAGCAGGACTGGGGTTTTGAAGGATGCAAAATTGTACAAATATGTGGTGACGTTAATAGGAATCTCTCGCGTGGAAATGATTCACGCAAAGGTGTTAAATCTTACAAAGTTTGGTTTATCGGATGATGAGATAGTTGGTCTTTTTGGTAAGTCTCCTAATATTATGACATTGTCAACTGATATGGCACAATGA

Protein sequence

>Msa0964080

MIFFDTALVIGPSGSGFGLQDGKITQGGGPGVPCEGESISEKIAAEMLHKTIARNPSLLCENGYDIKGIVAQYEELGVLKRDLVQMLILQPAIITRTSFDDEKMVYISRTGVLKDAKLYKYVVTLIGISRVEMIHAKVLNLTKFGLSDDEIVGLFGKSPNIMTLSTDMAQ*