AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0966100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0966100 MtrunA17_Chr2g0296781 72.829 714 161 4 8 689 3 715 0.0 1096
Msa0966100 MtrunA17_Chr6g0477261 71.894 644 138 5 117 718 1 643 0.0 950
Msa0966100 MtrunA17_Chr1g0196541 62.249 747 261 3 8 751 3 731 0.0 934
Msa0966100 MtrunA17_Chr5g0441581 46.753 616 318 3 2 616 32 638 0.0 580
Msa0966100 MtrunA17_Chr2g0291581 37.368 570 330 10 2 562 71 622 7.52e-125 389
Msa0966100 MtrunA17_Chr2g0329031 31.915 752 446 18 8 731 20 733 3.78e-121 380
Msa0966100 MtrunA17_Chr5g0448761 37.217 575 337 13 2 561 60 625 9.68e-120 378
Msa0966100 MtrunA17_Chr1g0207551 36.998 573 335 7 2 563 34 591 2.84e-119 377
Msa0966100 MtrunA17_Chr1g0202341 36.979 576 332 13 2 562 51 610 2.53e-117 369
Msa0966100 MtrunA17_Chr3g0080351 34.983 606 339 9 2 587 76 646 2.06e-115 368
Msa0966100 MtrunA17_Chr2g0291591 35.849 477 300 3 2 475 64 537 5.16e-102 325
Msa0966100 MtrunA17_Chr5g0440451 32.846 615 357 16 2 587 43 630 3.70e-96 319
Msa0966100 MtrunA17_Chr3g0118611 37.981 416 238 7 171 582 118 517 3.86e-87 291
Msa0966100 MtrunA17_Chr5g0448511 35.840 399 248 5 159 556 1 392 1.22e-85 281
Msa0966100 MtrunA17_Chr8g0363841 30.382 576 366 7 4 561 19 577 2.58e-83 278
Msa0966100 MtrunA17_Chr2g0324011 29.268 574 377 11 2 561 10 568 2.07e-79 266
Msa0966100 MtrunA17_Chr2g0287651 27.302 630 426 10 8 622 81 693 2.80e-78 269
Msa0966100 MtrunA17_Chr2g0287061 31.588 573 321 14 2 559 183 699 2.05e-75 260
Msa0966100 MtrunA17_Chr6g0476441 47.931 290 88 5 501 728 1 289 2.39e-75 245
Msa0966100 MtrunA17_Chr1g0198761 30.014 693 407 23 5 657 50 704 1.93e-74 256
Msa0966100 MtrunA17_Chr1g0203051 29.380 548 358 13 8 542 85 616 7.57e-70 241
Msa0966100 MtrunA17_Chr2g0276841 27.638 597 354 20 1 558 69 626 6.38e-65 229
Msa0966100 MtrunA17_Chr1g0203721 28.201 578 376 13 4 561 65 623 1.26e-62 226
Msa0966100 MtrunA17_Chr3g0082121 29.931 578 359 15 1 561 42 590 5.35e-62 221
Msa0966100 MtrunA17_Chr6g0458391 26.923 572 377 10 1 561 224 765 5.99e-62 222
Msa0966100 MtrunA17_Chr2g0294841 24.727 732 466 21 1 683 44 739 8.23e-56 204
Msa0966100 MtrunA17_Chr5g0431001 32.176 432 257 13 1 406 60 481 3.09e-54 195
Msa0966100 MtrunA17_Chr1g0164591 33.739 329 176 9 80 389 3 308 1.05e-46 169
Msa0966100 MtrunA17_Chr2g0290391 26.109 586 380 20 2 559 51 611 1.13e-45 174
Msa0966100 MtrunA17_Chr7g0258861 31.095 283 182 5 2 274 10 289 2.50e-40 150
Msa0966100 MtrunA17_Chr6g0476431 64.286 84 30 0 8 91 3 86 7.65e-32 119
Msa0966100 MtrunA17_Chr6g0451441 32.121 165 105 4 2 163 87 247 6.79e-19 90.5
Msa0966100 MtrunA17_Chr2g0308841 26.484 219 144 5 346 558 17 224 5.43e-15 75.9
Msa0966100 MtrunA17_Chr1g0172041 30.909 110 75 1 475 584 3 111 1.93e-14 70.1
Msa0966100 MtrunA17_Chr1g0149131 30.088 113 72 2 276 384 1 110 7.90e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0966100 AT4G15090.1 31.894 718 465 11 2 708 51 755 4.64e-125 394
Msa0966100 AT4G15090.5 31.894 718 465 11 2 708 51 755 4.64e-125 394
Msa0966100 AT4G15090.3 31.894 718 465 11 2 708 51 755 4.64e-125 394
Msa0966100 AT4G15090.2 31.894 718 465 11 2 708 51 755 4.64e-125 394
Msa0966100 AT4G15090.4 31.894 718 465 11 2 708 51 755 4.64e-125 394
Msa0966100 AT4G38180.1 35.951 573 343 10 2 561 73 634 9.68e-117 370
Msa0966100 AT1G76320.3 35.552 571 332 9 8 566 3 549 8.69e-113 357
Msa0966100 AT1G76320.2 35.552 571 332 9 8 566 3 549 1.72e-112 358
Msa0966100 AT1G76320.1 35.552 571 332 9 8 566 3 549 2.33e-112 357
Msa0966100 AT1G76320.4 35.552 571 332 9 8 566 3 549 2.33e-112 357
Msa0966100 AT1G52520.1 34.790 572 349 13 2 564 85 641 1.36e-110 352
Msa0966100 AT3G22170.2 34.423 581 334 9 2 564 70 621 2.72e-110 355
Msa0966100 AT3G22170.1 34.423 581 334 9 2 564 70 621 2.72e-110 355
Msa0966100 AT1G80010.1 33.275 574 364 11 2 562 95 662 1.11e-99 324
Msa0966100 AT4G19990.5 33.505 582 326 13 5 566 24 564 5.57e-94 309
Msa0966100 AT4G19990.3 33.505 582 326 13 5 566 24 564 6.18e-94 309
Msa0966100 AT4G19990.4 33.505 582 326 13 5 566 24 564 6.18e-94 309
Msa0966100 AT4G19990.6 33.505 582 326 13 5 566 24 564 9.19e-94 309
Msa0966100 AT4G19990.2 33.505 582 326 13 5 566 24 564 9.19e-94 309
Msa0966100 AT4G19990.7 33.505 582 326 13 5 566 38 578 1.29e-93 309
Msa0966100 AT2G27110.2 32.337 569 338 12 2 561 49 579 1.62e-88 297
Msa0966100 AT2G27110.1 32.337 569 338 12 2 561 49 579 1.62e-88 297
Msa0966100 AT4G19990.1 34.462 502 285 9 68 566 47 507 1.68e-88 293
Msa0966100 AT2G32250.8 34.921 441 268 7 178 613 135 561 1.26e-85 287
Msa0966100 AT2G32250.7 34.921 441 268 7 178 613 135 561 1.41e-85 287
Msa0966100 AT2G32250.5 34.921 441 268 7 178 613 176 602 1.43e-85 287
Msa0966100 AT2G32250.2 34.921 441 268 7 178 613 176 602 2.43e-85 287
Msa0966100 AT2G32250.4 34.921 441 268 7 178 613 176 602 2.43e-85 287
Msa0966100 AT2G32250.6 34.921 441 268 7 178 613 176 602 3.23e-85 287
Msa0966100 AT2G32250.1 34.921 441 268 7 178 613 176 602 3.23e-85 287
Msa0966100 AT2G32250.3 34.921 441 268 7 178 613 176 602 3.23e-85 287
Msa0966100 AT2G27110.3 35.374 441 260 9 126 561 14 434 2.46e-83 280
Msa0966100 AT4G38170.1 34.466 412 252 8 167 573 13 411 9.20e-78 260
Msa0966100 AT3G06250.2 30.249 562 339 11 2 559 190 702 1.20e-75 260
Msa0966100 AT3G06250.1 30.249 562 339 11 2 559 190 702 1.20e-75 260
Msa0966100 AT3G06250.3 30.249 562 339 11 2 559 190 702 1.20e-75 260
Msa0966100 AT5G18960.2 30.864 567 332 14 2 559 211 726 3.34e-75 259
Msa0966100 AT5G18960.1 30.864 567 332 14 2 559 211 726 3.34e-75 259
Msa0966100 AT1G10240.2 27.973 597 352 21 1 558 47 604 1.51e-64 228
Msa0966100 AT1G10240.1 27.973 597 352 21 1 558 47 604 1.51e-64 228
Msa0966100 AT5G28530.3 26.667 585 378 17 2 559 55 615 9.21e-50 186
Msa0966100 AT5G28530.2 26.667 585 378 17 2 559 55 615 9.21e-50 186
Msa0966100 AT5G28530.1 26.655 589 377 18 2 559 55 619 6.99e-49 184
Msa0966100 AT5G28530.5 24.433 573 343 19 2 559 55 552 1.33e-32 134
Msa0966100 AT5G28530.4 24.437 577 342 20 2 559 55 556 7.92e-32 132

Find 106 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTGGTGAATGCTTTC+TGG 0.174694 6_3:-94048950 None:intergenic
CCATCTTCTTCTATAATTTC+AGG 0.175398 6_3:-94049464 None:intergenic
CATATGTGAGAGACACATTT+TGG 0.184902 6_3:+94048724 Msa0966100:CDS
TATCAAAACCACATTTAATA+AGG 0.185554 6_3:-94049286 None:intergenic
TCACTCATAAGTATTGAATT+TGG 0.202186 6_3:-94049955 None:intergenic
CAACCTCCACTTGAAATTCT+TGG 0.215469 6_3:-94048977 None:intergenic
GTCGTTTAAGAAATGTATTT+TGG 0.215986 6_3:+94048187 Msa0966100:CDS
AAGGAAAACATGGCTGATTT+TGG 0.228183 6_3:+94048873 Msa0966100:CDS
CACCATCGATGGCGAGCTTT+TGG 0.238537 6_3:-94048492 None:intergenic
TTTGAGTAAAAGTGATTTCA+TGG 0.249102 6_3:+94048611 Msa0966100:CDS
ATAGCTTGTGTAAGTCATTT+TGG 0.262515 6_3:+94048912 Msa0966100:CDS
CGTAGAAGCAACTTCGTAAA+AGG 0.287052 6_3:-94049357 None:intergenic
TTCATGACTTGAGGATAAAA+TGG 0.287642 6_3:-94050073 None:intergenic
CTTTGTGTGCTTAAGCTAAC+AGG 0.298887 6_3:+94049194 Msa0966100:CDS
TATGCCCGGTCTATAGGTTT+TGG 0.300318 6_3:+94047652 Msa0966100:CDS
TGATCAGTTATTATGGCATT+TGG 0.323265 6_3:-94048432 None:intergenic
TGGTGAATGCTTTCTGGAAT+TGG 0.326923 6_3:-94048944 None:intergenic
CTCAAAGTCTTTCTCATTGA+AGG 0.328946 6_3:-94049117 None:intergenic
AGGGCTGCATATGAATATTT+TGG 0.330794 6_3:+94048225 Msa0966100:CDS
AAGTGCGGAGACTGCGACTT+GGG 0.331491 6_3:+94048073 Msa0966100:CDS
CGCAGTGGAGGAAGGATATA+AGG 0.337986 6_3:+94049252 Msa0966100:CDS
TTTGTGGCATATAATGAAAA+AGG 0.344495 6_3:+94048515 Msa0966100:CDS
AGTATGAACTCATTCTTTGA+TGG 0.348820 6_3:+94048777 Msa0966100:CDS
ATTGTCTTGATCAGTTATTA+TGG 0.355900 6_3:-94048439 None:intergenic
AAGAGTTATGCCCGGTCTAT+AGG 0.361091 6_3:+94047646 Msa0966100:CDS
ACTTATGAGTGAAGTATATC+AGG 0.377433 6_3:+94049966 Msa0966100:CDS
TCATGCTTGCTTTGATAGAT+TGG 0.388712 6_3:+94049016 Msa0966100:CDS
ATCAAAACCACATTTAATAA+GGG 0.391774 6_3:-94049285 None:intergenic
CATCTTCTTCTATAATTTCA+GGG 0.405722 6_3:-94049463 None:intergenic
CCTTTACTTCGAGTCGGTAC+AGG 0.409568 6_3:-94049521 None:intergenic
AATTAAAGACATAGTGTTTG+AGG 0.424296 6_3:+94049091 Msa0966100:CDS
AAAGTGATTTCATGGAAAAG+TGG 0.450883 6_3:+94048619 Msa0966100:CDS
AAGTATTGAATTTGGATTGA+AGG 0.452798 6_3:-94049947 None:intergenic
CTCGCCATCGATGGTGTTTG+TGG 0.452930 6_3:+94048499 Msa0966100:CDS
ATTTGATGAAAGAAATCGTT+GGG 0.454237 6_3:+94048695 Msa0966100:CDS
GAATATGACATTTGGTGAAA+AGG 0.456326 6_3:+94048029 Msa0966100:CDS
CCATGGTCAATCTGTGTTGT+TGG 0.460551 6_3:+94048326 Msa0966100:CDS
CATGGTCAATCTGTGTTGTT+GGG 0.466450 6_3:+94048327 Msa0966100:CDS
ACTCCAACAAAAGGAGCAAA+TGG 0.472118 6_3:-94048297 None:intergenic
TGTTGTTTGTTTCAATTCAA+AGG 0.475247 6_3:+94049152 Msa0966100:CDS
TTTGGGCAGATGCGAGATGT+AGG 0.483477 6_3:+94048205 Msa0966100:CDS
AAGGTCGTCCTCCTTCAAAA+AGG 0.483624 6_3:+94049540 Msa0966100:CDS
AGTATTGAATTTGGATTGAA+GGG 0.484124 6_3:-94049946 None:intergenic
TTGGCTGTTCACAACATGGT+TGG 0.487761 6_3:+94048389 Msa0966100:CDS
CCTGAAATTATAGAAGAAGA+TGG 0.488011 6_3:+94049464 Msa0966100:CDS
CTAAATATAGTTCCAACCTT+AGG 0.488331 6_3:-94047595 None:intergenic
TATCCTCAAGTCATGAATCA+TGG 0.488967 6_3:+94050079 Msa0966100:CDS
CGGAGACTGCGACTTGGGGT+TGG 0.494185 6_3:+94048078 Msa0966100:CDS
GTTCAAGTAGTTGATAAAGT+TGG 0.495312 6_3:+94049758 Msa0966100:CDS
CTATAGTTGTTGAAGCGAAT+GGG 0.503159 6_3:+94048001 Msa0966100:CDS
AAGTGATTTCATGGAAAAGT+GGG 0.506412 6_3:+94048620 Msa0966100:CDS
TCTATAGTTGTTGAAGCGAA+TGG 0.507984 6_3:+94048000 Msa0966100:CDS
CCACTGCGAGAAAATATAAG+AGG 0.509446 6_3:-94049237 None:intergenic
AATGTGTCTCTCACATATGC+AGG 0.509862 6_3:-94048720 None:intergenic
CATTCAATCTAGCCTTGCAC+TGG 0.510411 6_3:-94047801 None:intergenic
AAACACTATGTCTTTAATTG+TGG 0.512674 6_3:-94049087 None:intergenic
AATGCGTGTATTTCTTTAGA+CGG 0.517437 6_3:+94047820 Msa0966100:CDS
GCTTGCTTTGATAGATTGGA+AGG 0.517556 6_3:+94049020 Msa0966100:CDS
ATTGCTCTTGAGCATAATCA+TGG 0.525914 6_3:+94047862 Msa0966100:CDS
TGGTGATTTACTCCAACAAA+AGG 0.525957 6_3:-94048306 None:intergenic
TCAGGATAACGAATGGTTGA+AGG 0.526171 6_3:+94048668 Msa0966100:CDS
TGGATCCTCGTTTGAAAAGG+AGG 0.528466 6_3:+94047929 Msa0966100:CDS
GGGTATGAGAATATGACATT+TGG 0.528947 6_3:+94048021 Msa0966100:CDS
AAAGATGAAATGCCTAAGGT+TGG 0.530444 6_3:+94047583 None:intergenic
TTCCAAAAGCTCGCCATCGA+TGG 0.531681 6_3:+94048490 Msa0966100:CDS
TTGGGCAGATGCGAGATGTA+GGG 0.538615 6_3:+94048206 Msa0966100:CDS
TGTTGTGAACAGCCAAGTAA+AGG 0.547394 6_3:-94048382 None:intergenic
TAACTCCAAAACCTATAGAC+CGG 0.548272 6_3:-94047657 None:intergenic
AGGATACTAAGACCTTTACT+TGG 0.548532 6_3:+94048370 Msa0966100:CDS
CAGGATAACGAATGGTTGAA+GGG 0.548682 6_3:+94048669 Msa0966100:CDS
CCTGTACCGACTCGAAGTAA+AGG 0.550769 6_3:+94049521 Msa0966100:CDS
CTTTACTTCGAGTCGGTACA+GGG 0.551509 6_3:-94049520 None:intergenic
AGGCATACCCTTATTAAATG+TGG 0.553411 6_3:+94049278 Msa0966100:CDS
TTACTTGGCTGTTCACAACA+TGG 0.555090 6_3:+94048385 Msa0966100:CDS
ATTCCAAGAATTTCAAGTGG+AGG 0.555252 6_3:+94048974 Msa0966100:CDS
TTCAGGGCATGTTTCTTGAG+AGG 0.556231 6_3:-94049447 None:intergenic
ATTGAAAGATAAAGTTGAGA+AGG 0.561801 6_3:+94048854 Msa0966100:CDS
TTAGAACTCAATGATCAAGC+TGG 0.567016 6_3:+94047952 Msa0966100:CDS
AAAGTGCGGAGACTGCGACT+TGG 0.575984 6_3:+94048072 Msa0966100:CDS
TCGCTTCAACAACTATAGAT+CGG 0.588019 6_3:-94047996 None:intergenic
ACATGGTTGGAATGCATGCA+TGG 0.592737 6_3:+94048402 Msa0966100:CDS
TTGAACTTCAGGATAACGAA+TGG 0.592982 6_3:+94048661 Msa0966100:CDS
TCATAAAGATGAAATGCCTA+AGG 0.597014 6_3:+94047579 None:intergenic
ACACCATGATTCATGACTTG+AGG 0.597741 6_3:-94050082 None:intergenic
CATTTGGTGAAAAGGACTGT+AGG 0.603039 6_3:+94048037 Msa0966100:CDS
TATTTGATGAAAGAAATCGT+TGG 0.605775 6_3:+94048694 Msa0966100:CDS
AACTCCAAAACCTATAGACC+GGG 0.618933 6_3:-94047656 None:intergenic
CTGTTAGCTTAAGCACACAA+AGG 0.625605 6_3:-94049193 None:intergenic
TGCGAGAAAATATAAGAGGA+CGG 0.628214 6_3:-94049233 None:intergenic
AGTATTACAAGAGTTATGCC+CGG 0.630025 6_3:+94047638 Msa0966100:CDS
AGTGCGGAGACTGCGACTTG+GGG 0.633117 6_3:+94048074 Msa0966100:CDS
TAAAGTTGAGAAGGAAAACA+TGG 0.634633 6_3:+94048863 Msa0966100:CDS
TATGCCACAAACACCATCGA+TGG 0.638359 6_3:-94048503 None:intergenic
TTTACTCTAGCATGTAGTCG+TGG 0.642195 6_3:+94047721 Msa0966100:CDS
AAAATTCCAAGAATTTCAAG+TGG 0.644675 6_3:+94048971 Msa0966100:CDS
ACTTGGGGTTGGAGATGCTG+AGG 0.644897 6_3:+94048089 Msa0966100:CDS
GGAGGAAGGATATAAGGCGA+AGG 0.647816 6_3:+94049258 Msa0966100:CDS
AGGATAACGAATGGTTGAAG+GGG 0.650586 6_3:+94048670 Msa0966100:CDS
CTTGCAATTTAGATGCACAC+AGG 0.652836 6_3:-94049730 None:intergenic
TTTGTTGGAGTAAATCACCA+TGG 0.661689 6_3:+94048309 Msa0966100:CDS
TTTAGGAGGAATGAACATGG+TGG 0.670141 6_3:+94049907 Msa0966100:CDS
CGTGTTGTTGATGATGACGA+TGG 0.670761 6_3:-94050236 None:intergenic
ATGTGCTTTACTGTCAAGCG+AGG 0.675011 6_3:+94048350 Msa0966100:CDS
CCAACAACACAGATTGACCA+TGG 0.675112 6_3:-94048326 None:intergenic
GGACGACCTTTACTTCGAGT+CGG 0.678639 6_3:-94049527 None:intergenic
GGAACTATATAGATAAAGTG+CGG 0.685509 6_3:+94048058 Msa0966100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGAAGTAATAAGATTT+TGG + chr6_3:94047909-94047928 Msa0966100:CDS 15.0%
!!! TTTATTACATGTATGTTTTT+AGG + chr6_3:94049870-94049889 Msa0966100:intron 15.0%
!!! TTATTACATGTATGTTTTTA+GGG + chr6_3:94049871-94049890 Msa0966100:intron 15.0%
!!! TTCATATTTTGAATTATTAC+AGG + chr6_3:94050105-94050124 Msa0966100:CDS 15.0%
!! TTAATTAAAAGAAACATAAG+AGG - chr6_3:94050148-94050167 None:intergenic 15.0%
!!! TCGTTTAAGAAATGTATTTT+GGG + chr6_3:94048188-94048207 Msa0966100:CDS 20.0%
!!! TGATTTTGGTTCATTTAATA+CGG + chr6_3:94048887-94048906 Msa0966100:CDS 20.0%
!! ATCAAAACCACATTTAATAA+GGG - chr6_3:94049288-94049307 None:intergenic 20.0%
!! TATCAAAACCACATTTAATA+AGG - chr6_3:94049289-94049308 None:intergenic 20.0%
!!! TGTGGTTTTGATAATTTAAT+TGG + chr6_3:94049296-94049315 Msa0966100:CDS 20.0%
!!! GTCTAATTTTCAATATTTGA+TGG + chr6_3:94049702-94049721 Msa0966100:intron 20.0%
!!! TATTACATGTATGTTTTTAG+GGG + chr6_3:94049872-94049891 Msa0966100:intron 20.0%
! ACTAAACTTAGTAGCAAAAA+TGG + chr6_3:94047679-94047698 Msa0966100:CDS 25.0%
!!! TAAAATAGCGTGTTTTGTTA+GGG - chr6_3:94047894-94047913 None:intergenic 25.0%
!! GTCGTTTAAGAAATGTATTT+TGG + chr6_3:94048187-94048206 Msa0966100:CDS 25.0%
! ATTGTCTTGATCAGTTATTA+TGG - chr6_3:94048442-94048461 None:intergenic 25.0%
! TTTGTGGCATATAATGAAAA+AGG + chr6_3:94048515-94048534 Msa0966100:CDS 25.0%
! TTTGAGTAAAAGTGATTTCA+TGG + chr6_3:94048611-94048630 Msa0966100:CDS 25.0%
! TATTTGATGAAAGAAATCGT+TGG + chr6_3:94048694-94048713 Msa0966100:CDS 25.0%
! ATTTGATGAAAGAAATCGTT+GGG + chr6_3:94048695-94048714 Msa0966100:CDS 25.0%
! ATTGAAAGATAAAGTTGAGA+AGG + chr6_3:94048854-94048873 Msa0966100:CDS 25.0%
!! AAATTCTTGGAATTTTGCAT+TGG - chr6_3:94048967-94048986 None:intergenic 25.0%
! AAAATTCCAAGAATTTCAAG+TGG + chr6_3:94048971-94048990 Msa0966100:CDS 25.0%
! AAACACTATGTCTTTAATTG+TGG - chr6_3:94049090-94049109 None:intergenic 25.0%
! AATTAAAGACATAGTGTTTG+AGG + chr6_3:94049091-94049110 Msa0966100:CDS 25.0%
!! TGTTGTTTGTTTCAATTCAA+AGG + chr6_3:94049152-94049171 Msa0966100:CDS 25.0%
! CATCTTCTTCTATAATTTCA+GGG - chr6_3:94049466-94049485 None:intergenic 25.0%
!!! AATTTTCAATATTTGATGGC+AGG + chr6_3:94049706-94049725 Msa0966100:intron 25.0%
!!! ATTACATGTATGTTTTTAGG+GGG + chr6_3:94049873-94049892 Msa0966100:intron 25.0%
!! AGTATTGAATTTGGATTGAA+GGG - chr6_3:94049949-94049968 None:intergenic 25.0%
!!! AAGTATTGAATTTGGATTGA+AGG - chr6_3:94049950-94049969 None:intergenic 25.0%
!!! TCACTCATAAGTATTGAATT+TGG - chr6_3:94049958-94049977 None:intergenic 25.0%
!! AAGTATGTTTCCTGTTATTT+AGG + chr6_3:94050048-94050067 Msa0966100:intron 25.0%
! TAAAATGGAACCTAAATAAC+AGG - chr6_3:94050061-94050080 None:intergenic 25.0%
!!! CTGTCATTTTTTCTTTTTGA+AGG + chr6_3:94050193-94050212 Msa0966100:intron 25.0%
CTAAATATAGTTCCAACCTT+AGG - chr6_3:94047598-94047617 None:intergenic 30.0%
!! TTGCACTGGATTTTTGTTAT+AGG - chr6_3:94047790-94047809 None:intergenic 30.0%
TATAACAAAAATCCAGTGCA+AGG + chr6_3:94047789-94047808 Msa0966100:CDS 30.0%
AATGCGTGTATTTCTTTAGA+CGG + chr6_3:94047820-94047839 Msa0966100:CDS 30.0%
!! CTAAAATAGCGTGTTTTGTT+AGG - chr6_3:94047895-94047914 None:intergenic 30.0%
! GAATATGACATTTGGTGAAA+AGG + chr6_3:94048029-94048048 Msa0966100:CDS 30.0%
GGAACTATATAGATAAAGTG+CGG + chr6_3:94048058-94048077 Msa0966100:CDS 30.0%
TGATCAGTTATTATGGCATT+TGG - chr6_3:94048435-94048454 None:intergenic 30.0%
AAAGTGATTTCATGGAAAAG+TGG + chr6_3:94048619-94048638 Msa0966100:CDS 30.0%
AAGTGATTTCATGGAAAAGT+GGG + chr6_3:94048620-94048639 Msa0966100:CDS 30.0%
! GATTGAAGATTTTGAACTTC+AGG + chr6_3:94048650-94048669 Msa0966100:CDS 30.0%
! ATATGTGAGAGACACATTTT+GGG + chr6_3:94048725-94048744 Msa0966100:CDS 30.0%
AGTATGAACTCATTCTTTGA+TGG + chr6_3:94048777-94048796 Msa0966100:CDS 30.0%
TAAAGTTGAGAAGGAAAACA+TGG + chr6_3:94048863-94048882 Msa0966100:CDS 30.0%
!! ATAGCTTGTGTAAGTCATTT+TGG + chr6_3:94048912-94048931 Msa0966100:CDS 30.0%
CCATCTTCTTCTATAATTTC+AGG - chr6_3:94049467-94049486 None:intergenic 30.0%
CCTGAAATTATAGAAGAAGA+TGG + chr6_3:94049464-94049483 Msa0966100:CDS 30.0%
!!! ACTTACTTGGCTTCTTTTTT+TGG - chr6_3:94049614-94049633 None:intergenic 30.0%
AAAAAAAGAAGCCAAGTAAG+TGG + chr6_3:94049613-94049632 Msa0966100:intron 30.0%
! TTTGATGATTTCCACTTACT+TGG - chr6_3:94049627-94049646 None:intergenic 30.0%
AAATCATCAAAGTCAACAAG+AGG + chr6_3:94049636-94049655 Msa0966100:intron 30.0%
!! GTTCAAGTAGTTGATAAAGT+TGG + chr6_3:94049758-94049777 Msa0966100:CDS 30.0%
!!! TTACATGTATGTTTTTAGGG+GGG + chr6_3:94049874-94049893 Msa0966100:intron 30.0%
ACTTATGAGTGAAGTATATC+AGG + chr6_3:94049966-94049985 Msa0966100:CDS 30.0%
TTCCTGGAACATTATTATTG+TGG + chr6_3:94050017-94050036 Msa0966100:intron 30.0%
ATCCACAATAATAATGTTCC+AGG - chr6_3:94050022-94050041 None:intergenic 30.0%
TTCATGACTTGAGGATAAAA+TGG - chr6_3:94050076-94050095 None:intergenic 30.0%
AGTATTACAAGAGTTATGCC+CGG + chr6_3:94047638-94047657 Msa0966100:CDS 35.0%
TAACTCCAAAACCTATAGAC+CGG - chr6_3:94047660-94047679 None:intergenic 35.0%
AGTAGCAAAAATGGAGATGA+TGG + chr6_3:94047688-94047707 Msa0966100:CDS 35.0%
ATTGCTCTTGAGCATAATCA+TGG + chr6_3:94047862-94047881 Msa0966100:CDS 35.0%
! TTTTGGATCCTCGTTTGAAA+AGG + chr6_3:94047926-94047945 Msa0966100:CDS 35.0%
TTAGAACTCAATGATCAAGC+TGG + chr6_3:94047952-94047971 Msa0966100:CDS 35.0%
TCGCTTCAACAACTATAGAT+CGG - chr6_3:94047999-94048018 None:intergenic 35.0%
TCTATAGTTGTTGAAGCGAA+TGG + chr6_3:94048000-94048019 Msa0966100:CDS 35.0%
CTATAGTTGTTGAAGCGAAT+GGG + chr6_3:94048001-94048020 Msa0966100:CDS 35.0%
GGGTATGAGAATATGACATT+TGG + chr6_3:94048021-94048040 Msa0966100:CDS 35.0%
! AGGGCTGCATATGAATATTT+TGG + chr6_3:94048225-94048244 Msa0966100:CDS 35.0%
TGGTGATTTACTCCAACAAA+AGG - chr6_3:94048309-94048328 None:intergenic 35.0%
TTTGTTGGAGTAAATCACCA+TGG + chr6_3:94048309-94048328 Msa0966100:CDS 35.0%
AGGATACTAAGACCTTTACT+TGG + chr6_3:94048370-94048389 Msa0966100:CDS 35.0%
TGCCATGAAAAATGCAATTG+CGG + chr6_3:94048461-94048480 Msa0966100:CDS 35.0%
! ATCCGCAATTGCATTTTTCA+TGG - chr6_3:94048466-94048485 None:intergenic 35.0%
AAAAAGGTTCCAGAAAAGCT+AGG + chr6_3:94048531-94048550 Msa0966100:CDS 35.0%
TTGAACTTCAGGATAACGAA+TGG + chr6_3:94048661-94048680 Msa0966100:CDS 35.0%
! CATATGTGAGAGACACATTT+TGG + chr6_3:94048724-94048743 Msa0966100:CDS 35.0%
!! AAGGAAAACATGGCTGATTT+TGG + chr6_3:94048873-94048892 Msa0966100:CDS 35.0%
! ATTCCAAGAATTTCAAGTGG+AGG + chr6_3:94048974-94048993 Msa0966100:CDS 35.0%
TCATGCTTGCTTTGATAGAT+TGG + chr6_3:94049016-94049035 Msa0966100:CDS 35.0%
CTCAAAGTCTTTCTCATTGA+AGG - chr6_3:94049120-94049139 None:intergenic 35.0%
TGCGAGAAAATATAAGAGGA+CGG - chr6_3:94049236-94049255 None:intergenic 35.0%
AGGCATACCCTTATTAAATG+TGG + chr6_3:94049278-94049297 Msa0966100:CDS 35.0%
! CTTTTACGAAGTTGCTTCTA+CGG + chr6_3:94049358-94049377 Msa0966100:CDS 35.0%
! TTTGAGGACTTCCTTTTTGA+AGG - chr6_3:94049554-94049573 None:intergenic 35.0%
! CACAATTTGAGCAACTTTTG+AGG - chr6_3:94049570-94049589 None:intergenic 35.0%
GTGAATAAACTTGCGAAAAG+AGG + chr6_3:94049587-94049606 Msa0966100:intron 35.0%
!!! AGTTTTAGGAGGAATGAACA+TGG + chr6_3:94049904-94049923 Msa0966100:CDS 35.0%
TATCCTCAAGTCATGAATCA+TGG + chr6_3:94050079-94050098 Msa0966100:CDS 35.0%
AACTCCAAAACCTATAGACC+GGG - chr6_3:94047659-94047678 None:intergenic 40.0%
TTTACTCTAGCATGTAGTCG+TGG + chr6_3:94047721-94047740 Msa0966100:CDS 40.0%
! TCTAACCTCCTTTTCAAACG+AGG - chr6_3:94047937-94047956 None:intergenic 40.0%
! CATTTGGTGAAAAGGACTGT+AGG + chr6_3:94048037-94048056 Msa0966100:CDS 40.0%
!! ATGCCATTTGCTCCTTTTGT+TGG + chr6_3:94048294-94048313 Msa0966100:CDS 40.0%
ACTCCAACAAAAGGAGCAAA+TGG - chr6_3:94048300-94048319 None:intergenic 40.0%
! CATGGTCAATCTGTGTTGTT+GGG + chr6_3:94048327-94048346 Msa0966100:CDS 40.0%
TGTTGTGAACAGCCAAGTAA+AGG - chr6_3:94048385-94048404 None:intergenic 40.0%
TTACTTGGCTGTTCACAACA+TGG + chr6_3:94048385-94048404 Msa0966100:CDS 40.0%
TCAGGATAACGAATGGTTGA+AGG + chr6_3:94048668-94048687 Msa0966100:CDS 40.0%
CAGGATAACGAATGGTTGAA+GGG + chr6_3:94048669-94048688 Msa0966100:CDS 40.0%
AGGATAACGAATGGTTGAAG+GGG + chr6_3:94048670-94048689 Msa0966100:CDS 40.0%
AATGTGTCTCTCACATATGC+AGG - chr6_3:94048723-94048742 None:intergenic 40.0%
!! TGGTGAATGCTTTCTGGAAT+TGG - chr6_3:94048947-94048966 None:intergenic 40.0%
! TTGCATTGGTGAATGCTTTC+TGG - chr6_3:94048953-94048972 None:intergenic 40.0%
CAACCTCCACTTGAAATTCT+TGG - chr6_3:94048980-94048999 None:intergenic 40.0%
GCTTGCTTTGATAGATTGGA+AGG + chr6_3:94049020-94049039 Msa0966100:CDS 40.0%
! CTGTTAGCTTAAGCACACAA+AGG - chr6_3:94049196-94049215 None:intergenic 40.0%
CTTTGTGTGCTTAAGCTAAC+AGG + chr6_3:94049194-94049213 Msa0966100:CDS 40.0%
CCACTGCGAGAAAATATAAG+AGG - chr6_3:94049240-94049259 None:intergenic 40.0%
! CCTCTTATATTTTCTCGCAG+TGG + chr6_3:94049237-94049256 Msa0966100:CDS 40.0%
! CTTATATTTTCTCGCAGTGG+AGG + chr6_3:94049240-94049259 Msa0966100:CDS 40.0%
CGTAGAAGCAACTTCGTAAA+AGG - chr6_3:94049360-94049379 None:intergenic 40.0%
CTTGCAATTTAGATGCACAC+AGG - chr6_3:94049733-94049752 None:intergenic 40.0%
TTTAGGAGGAATGAACATGG+TGG + chr6_3:94049907-94049926 Msa0966100:CDS 40.0%
ACACCATGATTCATGACTTG+AGG - chr6_3:94050085-94050104 None:intergenic 40.0%
AAGAGTTATGCCCGGTCTAT+AGG + chr6_3:94047646-94047665 Msa0966100:CDS 45.0%
! TATGCCCGGTCTATAGGTTT+TGG + chr6_3:94047652-94047671 Msa0966100:CDS 45.0%
!! CATTCAATCTAGCCTTGCAC+TGG - chr6_3:94047804-94047823 None:intergenic 45.0%
TGGATCCTCGTTTGAAAAGG+AGG + chr6_3:94047929-94047948 Msa0966100:CDS 45.0%
CCAACAACACAGATTGACCA+TGG - chr6_3:94048329-94048348 None:intergenic 45.0%
CCATGGTCAATCTGTGTTGT+TGG + chr6_3:94048326-94048345 Msa0966100:CDS 45.0%
ATGTGCTTTACTGTCAAGCG+AGG + chr6_3:94048350-94048369 Msa0966100:CDS 45.0%
TTGGCTGTTCACAACATGGT+TGG + chr6_3:94048389-94048408 Msa0966100:CDS 45.0%
ACATGGTTGGAATGCATGCA+TGG + chr6_3:94048402-94048421 Msa0966100:CDS 45.0%
TATGCCACAAACACCATCGA+TGG - chr6_3:94048506-94048525 None:intergenic 45.0%
!! GAGTGTCTACCTAGCTTTTC+TGG - chr6_3:94048543-94048562 None:intergenic 45.0%
! TGTGAGAGACACATTTTGGG+CGG + chr6_3:94048728-94048747 Msa0966100:CDS 45.0%
! TATTTTCTCGCAGTGGAGGA+AGG + chr6_3:94049244-94049263 Msa0966100:CDS 45.0%
TTCAGGGCATGTTTCTTGAG+AGG - chr6_3:94049450-94049469 None:intergenic 45.0%
CTTTACTTCGAGTCGGTACA+GGG - chr6_3:94049523-94049542 None:intergenic 45.0%
AAGGTCGTCCTCCTTCAAAA+AGG + chr6_3:94049540-94049559 Msa0966100:CDS 45.0%
!! GAGGACTTCCTTTTTGAAGG+AGG - chr6_3:94049551-94049570 None:intergenic 45.0%
!!! AGGGGGGACACATTAGTTTT+AGG + chr6_3:94049890-94049909 Msa0966100:intron 45.0%
!!! GGGGACACATTAGTTTTAGG+AGG + chr6_3:94049893-94049912 Msa0966100:CDS 45.0%
! TCCAGTGCTCTTTAGTTTCC+TGG + chr6_3:94050001-94050020 Msa0966100:intron 45.0%
! TCCAGGAAACTAAAGAGCAC+TGG - chr6_3:94050005-94050024 None:intergenic 45.0%
!! CGTGTTGTTGATGATGACGA+TGG - chr6_3:94050239-94050258 None:intergenic 45.0%
TTTGGGCAGATGCGAGATGT+AGG + chr6_3:94048205-94048224 Msa0966100:CDS 50.0%
TTGGGCAGATGCGAGATGTA+GGG + chr6_3:94048206-94048225 Msa0966100:CDS 50.0%
TTCCAAAAGCTCGCCATCGA+TGG + chr6_3:94048490-94048509 Msa0966100:CDS 50.0%
! GTGAGAGACACATTTTGGGC+GGG + chr6_3:94048729-94048748 Msa0966100:CDS 50.0%
CGCAGTGGAGGAAGGATATA+AGG + chr6_3:94049252-94049271 Msa0966100:CDS 50.0%
GGAGGAAGGATATAAGGCGA+AGG + chr6_3:94049258-94049277 Msa0966100:CDS 50.0%
CCTTTACTTCGAGTCGGTAC+AGG - chr6_3:94049524-94049543 None:intergenic 50.0%
!! CCTGTACCGACTCGAAGTAA+AGG + chr6_3:94049521-94049540 Msa0966100:CDS 50.0%
GGACGACCTTTACTTCGAGT+CGG - chr6_3:94049530-94049549 None:intergenic 50.0%
AAAGTGCGGAGACTGCGACT+TGG + chr6_3:94048072-94048091 Msa0966100:CDS 55.0%
AAGTGCGGAGACTGCGACTT+GGG + chr6_3:94048073-94048092 Msa0966100:CDS 55.0%
ACTTGGGGTTGGAGATGCTG+AGG + chr6_3:94048089-94048108 Msa0966100:CDS 55.0%
! CACCATCGATGGCGAGCTTT+TGG - chr6_3:94048495-94048514 None:intergenic 55.0%
CTCGCCATCGATGGTGTTTG+TGG + chr6_3:94048499-94048518 Msa0966100:CDS 55.0%
AGTGCGGAGACTGCGACTTG+GGG + chr6_3:94048074-94048093 Msa0966100:CDS 60.0%
! CGGAGACTGCGACTTGGGGT+TGG + chr6_3:94048078-94048097 Msa0966100:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 94047592 94050268 94047592 ID=Msa0966100;Name=Msa0966100
chr6_3 mRNA 94047592 94050268 94047592 ID=Msa0966100-mRNA-1;Parent=Msa0966100;Name=Msa0966100-mRNA-1;_AED=0.27;_eAED=0.37;_QI=0|0.25|0|1|1|1|5|0|757
chr6_3 exon 94047592 94049586 94047592 ID=Msa0966100-mRNA-1:exon:17298;Parent=Msa0966100-mRNA-1
chr6_3 exon 94049728 94049799 94049728 ID=Msa0966100-mRNA-1:exon:17299;Parent=Msa0966100-mRNA-1
chr6_3 exon 94049892 94049987 94049892 ID=Msa0966100-mRNA-1:exon:17300;Parent=Msa0966100-mRNA-1
chr6_3 exon 94050070 94050126 94050070 ID=Msa0966100-mRNA-1:exon:17301;Parent=Msa0966100-mRNA-1
chr6_3 exon 94050215 94050268 94050215 ID=Msa0966100-mRNA-1:exon:17302;Parent=Msa0966100-mRNA-1
chr6_3 CDS 94047592 94049586 94047592 ID=Msa0966100-mRNA-1:cds;Parent=Msa0966100-mRNA-1
chr6_3 CDS 94049728 94049799 94049728 ID=Msa0966100-mRNA-1:cds;Parent=Msa0966100-mRNA-1
chr6_3 CDS 94049892 94049987 94049892 ID=Msa0966100-mRNA-1:cds;Parent=Msa0966100-mRNA-1
chr6_3 CDS 94050070 94050126 94050070 ID=Msa0966100-mRNA-1:cds;Parent=Msa0966100-mRNA-1
chr6_3 CDS 94050215 94050268 94050215 ID=Msa0966100-mRNA-1:cds;Parent=Msa0966100-mRNA-1
Gene Sequence

>Msa0966100

ATGCCTAAGGTTGGAACTATATTTAGCTCTAAAGAAGAACTTGAAGAGTATTACAAGAGTTATGCCCGGTCTATAGGTTTTGGAGTTACTAAACTTAGTAGCAAAAATGGAGATGATGGAAAAAAGTATTTTACTCTAGCATGTAGTCGTGGAACAAAGTATGTGAGTAAATCAAAGAAGCTTTTGAAGTCAAATCCTATAACAAAAATCCAGTGCAAGGCTAGATTGAATGCGTGTATTTCTTTAGACGGAACAGTTACTATTTCAAAAATTGCTCTTGAGCATAATCATGGTCTAAGCCCTAACAAAACACGCTATTTTAGAAGTAATAAGATTTTGGATCCTCGTTTGAAAAGGAGGTTAGAACTCAATGATCAAGCTGGAATAAATGTGAGCAGAAATTTCCGATCTATAGTTGTTGAAGCGAATGGGTATGAGAATATGACATTTGGTGAAAAGGACTGTAGGAACTATATAGATAAAGTGCGGAGACTGCGACTTGGGGTTGGAGATGCTGAGGCGATTCAAAATTATTTTGTTAAAATGCAAAAGCAAAATAGTCAATTCTATTATATAATAGACATTGATGATAAAAGTCGTTTAAGAAATGTATTTTGGGCAGATGCGAGATGTAGGGCTGCATATGAATATTTTGGTGATGTCGTAACATTTGACACTACTTACTTAACAAATAAATATGACATGCCATTTGCTCCTTTTGTTGGAGTAAATCACCATGGTCAATCTGTGTTGTTGGGATGTGCTTTACTGTCAAGCGAGGATACTAAGACCTTTACTTGGCTGTTCACAACATGGTTGGAATGCATGCATGGACGTGCTCCAAATGCCATAATAACTGATCAAGACAATGCCATGAAAAATGCAATTGCGGATGTTTTTCCAAAAGCTCGCCATCGATGGTGTTTGTGGCATATAATGAAAAAGGTTCCAGAAAAGCTAGGTAGACACTCTCATTATGAGTCTATCAAAGAACTTTTGCATGATGTTGTATACGATTCTTTGAGTAAAAGTGATTTCATGGAAAAGTGGGAAAGAATGATTGAAGATTTTGAACTTCAGGATAACGAATGGTTGAAGGGGCTATTTGATGAAAGAAATCGTTGGGTTCCTGCATATGTGAGAGACACATTTTGGGCGGGCATGTCAACGACTCAAAGAAGTGAAAGTATGAACTCATTCTTTGATGGTTATGTAAATTTAAAGACAACATTGAAGCAATTTGTTGAACAATATGACAATGCATTGAAAGATAAAGTTGAGAAGGAAAACATGGCTGATTTTGGTTCATTTAATACGGTGATAGCTTGTGTAAGTCATTTTGGCTTTGAGTTCCAATTCCAGAAAGCATTCACCAATGCAAAATTCCAAGAATTTCAAGTGGAGGTTGCTTCTATGATTTATTGTCATGCTTGCTTTGATAGATTGGAAGGATCGAATTCTATATATTCTGTTATAGAAAGCAAGAAAAAATTTGCCACAATTAAAGACATAGTGTTTGAGGTTTCCTTCAATGAGAAAGACTTTGAGTTACAATGTATGTGTTGTTTGTTTCAATTCAAAGGTATTTTATGCAGACACATCCTTTGTGTGCTTAAGCTAACAGGAAAAACAGAGTCATTGCCGTCCTCTTATATTTTCTCGCAGTGGAGGAAGGATATAAGGCGAAGGCATACCCTTATTAAATGTGGTTTTGATAATTTAATTGGAAATGCTGAATTGCAAAGAGTTGATAAAGCTTGTTCTGCCTTTTACGAAGTTGCTTCTACGGTGATAAATAACGATAATGATTTGTCGAAAATGATGAATCTAATCAAGAAAATAAAAAGTGAGTTTACCTCTCAAGAAACATGCCCTGAAATTATAGAAGAAGATGGTTTAGTTCATAATCAAGTGACTAAAATTCTTGACCCTGTACCGACTCGAAGTAAAGGTCGTCCTCCTTCAAAAAGGAAGTCCTCAAAAGTTGCTCAAATTGACCTGTGTGCATCTAAATTGCAAGATAGTGTTCAAGTAGTTGATAAAGTTGGCACACAAGAGAGCATTCAAGGGGGACACATTAGTTTTAGGAGGAATGAACATGGTGGAGTAAATAATTTAGCTCCCTTCAATCCAAATTCAATACTTATGAGTGAAGTATATCAGGTTCCATTTTATCCTCAAGTCATGAATCATGGTGTTTCATATTTTGAATTATTACAGGCACAACATCATGTGAATGATCCATCGTCATCATCAACAACACGTGAATCATAA
Protein sequence

>Msa0966100

MPKVGTIFSSKEELEEYYKSYARSIGFGVTKLSSKNGDDGKKYFTLACSRGTKYVSKSKKLLKSNPITKIQCKARLNACISLDGTVTISKIALEHNHGLSPNKTRYFRSNKILDPRLKRRLELNDQAGINVSRNFRSIVVEANGYENMTFGEKDCRNYIDKVRRLRLGVGDAEAIQNYFVKMQKQNSQFYYIIDIDDKSRLRNVFWADARCRAAYEYFGDVVTFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSSEDTKTFTWLFTTWLECMHGRAPNAIITDQDNAMKNAIADVFPKARHRWCLWHIMKKVPEKLGRHSHYESIKELLHDVVYDSLSKSDFMEKWERMIEDFELQDNEWLKGLFDERNRWVPAYVRDTFWAGMSTTQRSESMNSFFDGYVNLKTTLKQFVEQYDNALKDKVEKENMADFGSFNTVIACVSHFGFEFQFQKAFTNAKFQEFQVEVASMIYCHACFDRLEGSNSIYSVIESKKKFATIKDIVFEVSFNEKDFELQCMCCLFQFKGILCRHILCVLKLTGKTESLPSSYIFSQWRKDIRRRHTLIKCGFDNLIGNAELQRVDKACSAFYEVASTVINNDNDLSKMMNLIKKIKSEFTSQETCPEIIEEDGLVHNQVTKILDPVPTRSKGRPPSKRKSSKVAQIDLCASKLQDSVQVVDKVGTQESIQGGHISFRRNEHGGVNNLAPFNPNSILMSEVYQVPFYPQVMNHGVSYFELLQAQHHVNDPSSSSTTRES*