AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0969310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0969310 MtrunA17_Chr6g0449311 88.889 144 13 1 37 177 1 144 2.34e-74 222
Msa0969310 MtrunA17_Chr5g0439111 63.924 158 45 4 1 153 1 151 5.20e-59 186
Msa0969310 MtrunA17_Chr6g0452661 47.034 236 71 7 1 233 1 185 1.98e-56 180
Msa0969310 MtrunA17_Chr4g0055441 61.871 139 39 3 10 145 3 130 3.79e-52 166
Msa0969310 MtrunA17_Chr6g0452691 55.405 148 53 3 1 142 1 141 6.04e-51 164
Msa0969310 MtrunA17_Chr6g0452671 54.930 142 57 3 1 140 1 137 2.09e-45 150
Msa0969310 MtrunA17_Chr4g0059901 50.943 106 51 1 8 113 9 113 1.09e-30 114
Msa0969310 MtrunA17_Chr8g0342351 52.381 105 49 1 8 112 11 114 3.23e-29 110
Msa0969310 MtrunA17_Chr4g0058651 38.806 134 70 2 7 140 9 130 9.73e-26 99.8
Msa0969310 MtrunA17_Chr3g0097221 45.714 105 56 1 7 111 5 108 1.69e-25 101
Msa0969310 MtrunA17_Chr7g0227511 44.898 98 53 1 7 104 9 105 5.66e-25 97.1
Msa0969310 MtrunA17_Chr2g0330201 45.283 106 57 1 8 113 18 122 4.42e-24 97.8
Msa0969310 MtrunA17_Chr5g0402611 42.857 105 59 1 7 111 6 109 6.01e-24 97.8
Msa0969310 MtrunA17_Chr8g0353981 48.193 83 42 1 7 89 5 86 8.98e-24 95.5
Msa0969310 MtrunA17_Chr3g0115431 43.137 102 57 1 10 111 10 110 1.08e-23 95.1
Msa0969310 MtrunA17_Chr3g0117121 43.878 98 54 1 7 104 8 104 2.90e-23 93.2
Msa0969310 MtrunA17_Chr4g0043421 38.168 131 79 2 10 140 16 144 5.47e-23 92.0
Msa0969310 MtrunA17_Chr6g0449641 41.837 98 56 1 7 104 7 103 4.82e-22 88.6
Msa0969310 MtrunA17_Chr5g0437571 37.931 116 70 2 7 122 4 117 7.68e-22 88.6
Msa0969310 MtrunA17_Chr2g0311601 39.796 98 58 1 10 107 14 110 2.67e-21 87.0
Msa0969310 MtrunA17_Chr5g0444351 30.556 144 96 2 10 153 6 145 4.56e-21 86.3
Msa0969310 MtrunA17_Chr6g0449651 40.816 98 57 1 7 104 65 161 5.36e-21 87.4
Msa0969310 MtrunA17_Chr5g0437401 35.780 109 69 1 5 113 7 114 1.10e-20 85.9
Msa0969310 MtrunA17_Chr3g0106101 36.937 111 68 2 10 120 8 116 1.76e-20 85.5
Msa0969310 MtrunA17_Chr4g0030001 39.655 116 67 3 10 124 25 138 1.88e-20 85.9
Msa0969310 MtrunA17_Chr5g0438091 31.618 136 86 2 10 139 40 174 3.60e-20 85.1
Msa0969310 MtrunA17_Chr7g0225111 36.364 110 69 1 10 119 6 114 5.20e-20 82.4
Msa0969310 MtrunA17_Chr8g0374591 38.462 104 63 1 8 111 7 109 7.31e-20 82.4
Msa0969310 MtrunA17_Chr1g0200201 37.500 128 70 3 10 129 73 198 2.48e-19 83.6
Msa0969310 MtrunA17_Chr8g0374601 39.423 104 62 1 8 111 25 127 2.66e-19 84.7
Msa0969310 MtrunA17_Chr4g0040831 32.558 129 78 3 8 132 14 137 3.69e-19 82.4
Msa0969310 MtrunA17_Chr3g0114061 39.048 105 62 2 10 114 9 111 5.67e-19 80.9
Msa0969310 MtrunA17_Chr3g0113891 37.500 104 64 1 5 108 10 112 1.24e-18 80.9
Msa0969310 MtrunA17_Chr3g0129071 36.735 98 61 1 10 107 48 144 3.32e-18 80.9
Msa0969310 MtrunA17_Chr5g0432451 31.429 140 84 2 10 149 44 171 6.69e-18 79.7
Msa0969310 MtrunA17_Chr7g0260971 38.835 103 61 2 10 111 15 116 1.67e-16 75.9
Msa0969310 MtrunA17_Chr8g0351721 35.185 108 68 2 5 111 14 120 7.27e-15 71.2
Msa0969310 MtrunA17_Chr1g0184281 34.783 115 71 3 10 121 16 129 2.09e-14 69.7
Msa0969310 MtrunA17_Chr7g0245871 35.354 99 62 2 10 107 28 125 2.90e-14 70.1
Msa0969310 MtrunA17_Chr8g0351701 33.981 103 66 2 10 111 24 125 5.32e-14 69.3
Msa0969310 MtrunA17_Chr4g0046201 37.500 88 54 1 9 96 6 92 6.77e-14 69.3
Msa0969310 MtrunA17_Chr6g0460951 33.333 102 66 2 10 110 41 141 1.38e-13 67.8
Msa0969310 MtrunA17_Chr8g0374581 34.615 104 66 2 8 110 7 109 1.04e-12 65.5
Msa0969310 MtrunA17_Chr1g0184271 33.000 100 65 2 10 108 13 111 1.24e-12 65.5
Msa0969310 MtrunA17_Chr6g0460941 35.227 88 55 2 10 96 8 94 2.07e-12 61.6
Msa0969310 MtrunA17_Chr5g0435641 28.302 106 74 2 7 111 5 109 5.96e-12 62.8
Msa0969310 MtrunA17_Chr2g0326041 50.000 48 24 0 10 57 34 81 3.34e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0969310 AT3G47870.1 55.769 104 45 1 9 112 36 138 3.33e-34 125
Msa0969310 AT3G13850.1 43.243 148 79 3 8 152 35 180 1.92e-29 111
Msa0969310 AT3G27650.1 42.424 99 56 1 7 105 37 134 3.13e-23 92.4
Msa0969310 AT5G63090.4 43.878 98 54 1 7 104 9 105 7.12e-23 92.4
Msa0969310 AT5G63090.3 43.878 98 54 1 7 104 9 105 7.12e-23 92.4
Msa0969310 AT5G63090.2 43.878 98 54 1 7 104 9 105 7.12e-23 92.4
Msa0969310 AT5G63090.1 43.878 98 54 1 7 104 9 105 7.12e-23 92.4
Msa0969310 AT1G65620.1 46.988 83 43 1 7 89 7 88 2.30e-22 91.3
Msa0969310 AT1G65620.4 46.988 83 43 1 7 89 7 88 2.30e-22 91.3
Msa0969310 AT1G65620.3 46.988 83 43 1 7 89 7 88 2.30e-22 91.3
Msa0969310 AT1G65620.5 46.988 83 43 1 7 89 7 88 2.30e-22 91.3
Msa0969310 AT1G65620.2 46.988 83 43 1 7 89 7 88 2.30e-22 91.3
Msa0969310 AT5G66870.1 41.176 102 59 1 10 111 8 108 9.18e-22 92.0
Msa0969310 AT3G26660.1 38.261 115 68 2 8 120 4 117 1.30e-21 87.0
Msa0969310 AT1G31320.1 45.263 95 48 2 5 96 6 99 2.95e-21 87.8
Msa0969310 AT3G26620.1 37.143 105 65 1 8 112 4 107 1.37e-20 84.3
Msa0969310 AT1G07900.1 39.130 115 68 2 10 124 34 146 2.10e-20 85.9
Msa0969310 AT1G72980.1 41.071 112 64 2 9 119 13 123 3.83e-20 85.9
Msa0969310 AT2G30130.1 38.596 114 68 2 10 123 9 120 3.37e-19 82.8
Msa0969310 AT2G28500.1 37.391 115 70 2 10 124 56 168 1.07e-18 82.4
Msa0969310 AT2G30340.2 38.776 98 59 1 10 107 54 150 1.95e-18 82.4
Msa0969310 AT2G30340.1 38.776 98 59 1 10 107 53 149 2.13e-18 82.0
Msa0969310 AT2G23660.2 40.594 101 59 1 10 110 6 105 7.74e-18 81.3
Msa0969310 AT2G23660.3 40.594 101 59 1 10 110 6 105 7.74e-18 81.3
Msa0969310 AT2G23660.1 40.594 101 59 1 10 110 6 105 7.74e-18 81.3
Msa0969310 AT2G40470.2 37.755 98 60 1 10 107 21 117 8.25e-18 79.3
Msa0969310 AT3G11090.1 35.354 99 63 1 5 103 7 104 8.54e-18 78.2
Msa0969310 AT1G16530.1 36.283 113 63 3 5 109 10 121 1.63e-17 77.8
Msa0969310 AT2G40470.1 37.755 98 60 1 10 107 46 142 2.34e-17 78.6
Msa0969310 AT5G15060.1 45.946 74 39 1 10 82 18 91 4.30e-17 75.9
Msa0969310 AT3G50510.1 36.449 107 66 2 10 115 13 118 1.17e-15 73.6
Msa0969310 AT3G50510.2 36.449 107 66 2 10 115 13 118 1.17e-15 73.6
Msa0969310 AT1G06280.1 38.298 94 53 3 8 101 23 111 1.43e-15 73.2
Msa0969310 AT2G19820.1 34.343 99 62 1 1 96 1 99 2.85e-15 70.5
Msa0969310 AT2G42430.1 28.966 145 101 2 10 153 16 159 8.43e-15 72.0
Msa0969310 AT4G00210.1 33.333 138 84 4 10 145 12 143 1.74e-14 70.5
Msa0969310 AT3G58190.1 33.645 107 69 2 10 115 12 117 1.05e-13 68.6
Msa0969310 AT2G42440.1 31.481 108 72 2 10 116 8 114 1.18e-13 68.9
Msa0969310 AT4G00220.1 34.653 101 64 2 12 111 20 119 1.98e-13 67.8
Msa0969310 AT2G45420.1 33.663 101 65 2 12 111 40 139 9.41e-13 66.6
Msa0969310 AT3G03760.1 34.314 102 65 2 10 110 52 152 2.18e-12 65.5
Msa0969310 AT5G35900.1 28.431 102 72 1 10 111 6 106 7.92e-12 63.2
Msa0969310 AT2G45410.1 35.644 101 63 2 12 111 19 118 2.13e-11 61.6
Msa0969310 AT2G31310.1 28.689 122 77 3 10 122 8 128 2.14e-11 61.6

Find 27 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCAACATTGCTAGATAT+TGG 0.233878 6_4:-811327 Msa0969300:intron
TCACCAACATTGTTAAATAT+TGG 0.234385 6_4:-811136 Msa0969300:intron
CTTTCTTCTTTGATATTTGC+AGG 0.243555 6_4:-810753 Msa0969300:intron
ATTATTATGTGATAATAATA+AGG 0.272056 6_4:-811163 Msa0969300:intron
TGGATGTCTTGGTCTTATTA+TGG 0.281895 6_4:+810967 Msa0969310:CDS
GGTTTATCGGCTGGAAAATA+AGG 0.296290 6_4:-810800 Msa0969300:intron
ATAATGAATACTCAAGTTCT+TGG 0.299220 6_4:+811181 Msa0969310:CDS
GCCAATGCTCATCGTTTGTT+TGG 0.347008 6_4:+810833 Msa0969310:CDS
TAAGGAGCTAAAGGGAAATC+TGG 0.373984 6_4:-810782 Msa0969300:intron
ATTCCAATATTTAACAATGT+TGG 0.448330 6_4:+811133 Msa0969310:CDS
TCTTCTACATTTCTCTTCAT+CGG 0.464845 6_4:+811078 Msa0969310:CDS
GGAATTGAAGAACTAGTAGA+AGG 0.471090 6_4:-811115 Msa0969300:intron
TTCCCAAGTCATGGATGTCT+TGG 0.482571 6_4:+810956 Msa0969310:CDS
GCTGGAAAATAAGGAGCTAA+AGG 0.488184 6_4:-810791 Msa0969300:intron
CTGGAAAATAAGGAGCTAAA+GGG 0.491954 6_4:-810790 Msa0969300:intron
GAATGATCCATTGAAAAGTA+AGG 0.496518 6_4:-811277 Msa0969300:intron
AATCCAATATCTAGCAATGT+TGG 0.505027 6_4:+811324 Msa0969310:CDS
ATGGATACAACAAGGGTACA+TGG 0.514070 6_4:+811409 Msa0969310:CDS
AGACCAAGACATCCATGACT+TGG 0.541471 6_4:-810959 Msa0969300:intron
ACCAAACAAACGATGAGCAT+TGG 0.553402 6_4:-810834 Msa0969300:intron
CGTGCAAGATTCCCAAGTCA+TGG 0.565788 6_4:+810947 Msa0969310:CDS
TACATGGTTGCACAACTGAT+TGG 0.568443 6_4:+811425 Msa0969310:CDS
GTAAATTTGTTAACATCGTG+AGG 0.570877 6_4:-811358 Msa0969300:intron
GACCAAGACATCCATGACTT+GGG 0.599785 6_4:-810958 Msa0969300:intron
ATATTGGATTTGAGAACTAG+TGG 0.602290 6_4:-811311 Msa0969300:intron
TTTGTTTGGTATTTCCAACA+TGG 0.612102 6_4:+810847 Msa0969310:CDS
TGGATTTGAGAACTAGTGGT+AGG 0.612330 6_4:-811307 Msa0969300:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATGTGATAATAATA+AGG - chr6_4:811166-811185 Msa0969300:intron 10.0%
!!! AAATTCGAATATAAATTTTA+TGG + chr6_4:811390-811409 Msa0969310:CDS 10.0%
!! ATTCCAATATTTAACAATGT+TGG + chr6_4:811133-811152 Msa0969310:CDS 20.0%
!!! TAAATTTTATGGATACAACA+AGG + chr6_4:811401-811420 Msa0969310:CDS 20.0%
!!! AAATTTTATGGATACAACAA+GGG + chr6_4:811402-811421 Msa0969310:CDS 20.0%
!!! GAAGTCAATTATTTTTGAGT+CGG + chr6_4:810919-810938 Msa0969310:CDS 25.0%
! TCACCAACATTGTTAAATAT+TGG - chr6_4:811139-811158 Msa0969300:intron 25.0%
! ATAATGAATACTCAAGTTCT+TGG + chr6_4:811181-811200 Msa0969310:CDS 25.0%
!!! TTTTCTACCTTACTTTTCAA+TGG + chr6_4:811270-811289 Msa0969310:CDS 25.0%
GGATATTCACAGAAAAAACA+AGG + chr6_4:810721-810740 Msa0969310:CDS 30.0%
CTTTCTTCTTTGATATTTGC+AGG - chr6_4:810756-810775 Msa0969300:intron 30.0%
TTTGTTTGGTATTTCCAACA+TGG + chr6_4:810847-810866 Msa0969310:CDS 30.0%
TCTTCTACATTTCTCTTCAT+CGG + chr6_4:811078-811097 Msa0969310:CDS 30.0%
GAATGATCCATTGAAAAGTA+AGG - chr6_4:811280-811299 Msa0969300:intron 30.0%
ATATTGGATTTGAGAACTAG+TGG - chr6_4:811314-811333 Msa0969300:intron 30.0%
AATCCAATATCTAGCAATGT+TGG + chr6_4:811324-811343 Msa0969310:CDS 30.0%
TTACCAACATTGCTAGATAT+TGG - chr6_4:811330-811349 Msa0969300:intron 30.0%
!! GTAAATTTGTTAACATCGTG+AGG - chr6_4:811361-811380 Msa0969300:intron 30.0%
CTGGAAAATAAGGAGCTAAA+GGG - chr6_4:810793-810812 Msa0969300:intron 35.0%
! TTTTAGCATCTTCACCATGT+TGG - chr6_4:810864-810883 Msa0969300:intron 35.0%
!! TGGATGTCTTGGTCTTATTA+TGG + chr6_4:810967-810986 Msa0969310:CDS 35.0%
GGAATTGAAGAACTAGTAGA+AGG - chr6_4:811118-811137 Msa0969300:intron 35.0%
TAAGGAGCTAAAGGGAAATC+TGG - chr6_4:810785-810804 Msa0969300:intron 40.0%
GCTGGAAAATAAGGAGCTAA+AGG - chr6_4:810794-810813 Msa0969300:intron 40.0%
! GGTTTATCGGCTGGAAAATA+AGG - chr6_4:810803-810822 Msa0969300:intron 40.0%
!!! AGCATTTTTGGTTTATCGGC+TGG - chr6_4:810812-810831 Msa0969300:intron 40.0%
!!! GGCGAGCATTTTTGGTTTAT+CGG - chr6_4:810816-810835 Msa0969300:intron 40.0%
ACCAAACAAACGATGAGCAT+TGG - chr6_4:810837-810856 Msa0969300:intron 40.0%
TGGATTTGAGAACTAGTGGT+AGG - chr6_4:811310-811329 Msa0969300:intron 40.0%
! ATGGATACAACAAGGGTACA+TGG + chr6_4:811409-811428 Msa0969310:CDS 40.0%
TACATGGTTGCACAACTGAT+TGG + chr6_4:811425-811444 Msa0969310:CDS 40.0%
!!! TGAGCATTGGCGAGCATTTT+TGG - chr6_4:810824-810843 Msa0969300:intron 45.0%
GCCAATGCTCATCGTTTGTT+TGG + chr6_4:810833-810852 Msa0969310:CDS 45.0%
TTCCCAAGTCATGGATGTCT+TGG + chr6_4:810956-810975 Msa0969310:CDS 45.0%
GACCAAGACATCCATGACTT+GGG - chr6_4:810961-810980 Msa0969300:intron 45.0%
AGACCAAGACATCCATGACT+TGG - chr6_4:810962-810981 Msa0969300:intron 45.0%
CGTGCAAGATTCCCAAGTCA+TGG + chr6_4:810947-810966 Msa0969310:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 810719 811450 810719 ID=Msa0969310;Name=Msa0969310
chr6_4 mRNA 810719 811450 810719 ID=Msa0969310-mRNA-1;Parent=Msa0969310;Name=Msa0969310-mRNA-1;_AED=0.02;_eAED=0.09;_QI=0|-1|0|1|-1|1|1|0|243
chr6_4 exon 810719 811450 810719 ID=Msa0969310-mRNA-1:exon:395;Parent=Msa0969310-mRNA-1
chr6_4 CDS 810719 811450 810719 ID=Msa0969310-mRNA-1:cds;Parent=Msa0969310-mRNA-1
Gene Sequence

>Msa0969310

ATGGATATTCACAGAAAAAACAAGGCATGTGCAGCCTGCAAATATCAAAGAAGAAAGTGTACTCCAGATTTCCCTTTAGCTCCTTATTTTCCAGCCGATAAACCAAAAATGCTCGCCAATGCTCATCGTTTGTTTGGTATTTCCAACATGGTGAAGATGCTAAAATCTATTGACGATGATGATCATAAAGATGAAGCAATGAAGTCAATTATTTTTGAGTCGGAAATGCGTGCAAGATTCCCAAGTCATGGATGTCTTGGTCTTATTATGGAGTATCAAGATATGATAATTGAGTCTATGAAAGAGTTAGATCATGTAAAACAGTTACTAAGTTTGTGTATGCTATCACAACAACAAAATCTTCTACATTTCTCTTCATCGGATCATTCTTCAGTACCTTCTACTAGTTCTTCAATTCCAATATTTAACAATGTTGGTGATGTTCCTTATTATTATCACATAATAATGAATACTCAAGTTCTTGGAAATCTTGATATGATAAAGTATGAAGAAGAGTTAGATTATGTAAAAAATAATCTACAGCAGCAAAATTTTCTACCTTACTTTTCAATGGATCATTCTTCTGTACCTACCACTAGTTCTCAAATCCAATATCTAGCAATGTTGGTAACATTCATTCCTCACGATGTTAACAAATTTACATCATATACAAATTCGAATATAAATTTTATGGATACAACAAGGGTACATGGTTGCACAACTGATTGGTGA

Protein sequence

>Msa0969310

MDIHRKNKACAACKYQRRKCTPDFPLAPYFPADKPKMLANAHRLFGISNMVKMLKSIDDDDHKDEAMKSIIFESEMRARFPSHGCLGLIMEYQDMIIESMKELDHVKQLLSLCMLSQQQNLLHFSSSDHSSVPSTSSSIPIFNNVGDVPYYYHIIMNTQVLGNLDMIKYEEELDYVKNNLQQQNFLPYFSMDHSSVPTTSSQIQYLAMLVTFIPHDVNKFTSYTNSNINFMDTTRVHGCTTDW*