AlfalfaGEDB | Msa0969740

M. sativa cultivar zhongmu-4 / Msa0969740

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0969740	KEH24761.1	97.143	105	3	0	2	106	210	314	1.57e-69	220

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0969740	A0A072U5Y5	97.143	105	3	0	2	106	210	314	7.48e-70	220

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0969740	MtrunA17_Chr6g0449241	97.143	105	3	0	2	106	248	352	7.56e-73	219
Msa0969740	MtrunA17_Chr3g0116981	58.095	105	44	0	2	106	248	352	7.36e-43	143

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0969740	AT5G50180.1	60.000	105	42	0	2	106	242	346	7.14e-43	143
Msa0969740	AT5G40540.3	65.385	104	33	2	2	102	151	254	4.73e-37	125
Msa0969740	AT5G40540.2	65.385	104	33	2	2	102	172	275	7.67e-37	125
Msa0969740	AT5G40540.1	65.385	104	33	2	2	102	248	351	3.75e-36	125
Msa0969740	AT3G27560.1	61.905	105	34	2	2	100	248	352	8.99e-35	122

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Find 32 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTAATGCACTTGTAC+CGG	0.492205	6_4:-1166747	None:intergenic
AAATCATACAGATGCTCCTT+CGG	0.494850	6_4:+1166639	Msa0969740:CDS
AAATGGTAGAGAGATACCGA+AGG	0.694007	6_4:-1166655	None:intergenic
AACATTAATGCACTTGTACC+GGG	0.545157	6_4:-1166746	None:intergenic
AACCGGTTCTAATGGCGAAA+TGG	0.471939	6_4:-1166672	None:intergenic
AATGTGGAAGACAGGTTCAA+AGG	0.531304	6_4:+1166803	Msa0969740:CDS
ACAATCATAGCTAAATCACC+AGG	0.643445	6_4:-1166569	None:intergenic
ACCGGTTGTTCCAATGCGGA+TGG	0.555137	6_4:+1166688	Msa0969740:CDS
AGAAACCCCGAAAGCCAATG+TGG	0.669643	6_4:+1166787	Msa0969740:CDS
ATGTGGAAGACAGGTTCAAA+GGG	0.506061	6_4:+1166804	Msa0969740:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTTTAGACAATAGGTAAAC+CGG	-	chr6_4:1166343-1166362	None:intergenic	30.0%
ACACAATCAGTTTAGACAAT+AGG	-	chr6_4:1166351-1166370	None:intergenic	30.0%
CTATGATTGTAACATCATGT+TGG	+	chr6_4:1166582-1166601	Msa0969740:CDS	30.0%
AAACATTAATGCACTTGTAC+CGG	-	chr6_4:1166750-1166769	None:intergenic	30.0%
AGCATGCTGACATATATTCT+AGG	+	chr6_4:1166177-1166196	Msa0969740:intron	35.0%
AAAGCTACTTGAGACGTATA+TGG	+	chr6_4:1166392-1166411	Msa0969740:intron	35.0%
GACGTATATGGTTTCTATTC+TGG	+	chr6_4:1166404-1166423	Msa0969740:intron	35.0%
ACAATCATAGCTAAATCACC+AGG	-	chr6_4:1166572-1166591	None:intergenic	35.0%
TGTAACATCATGTTGGAAAG+AGG	+	chr6_4:1166589-1166608	Msa0969740:CDS	35.0%
CTAAAATTCGGCCTATCATT+TGG	-	chr6_4:1166617-1166636	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	1166140	1166856	1166140	ID=Msa0969740;Name=Msa0969740
chr6_4	mRNA	1166140	1166856	1166140	ID=Msa0969740-mRNA-1;Parent=Msa0969740;Name=Msa0969740-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|106
chr6_4	exon	1166140	1166145	1166140	ID=Msa0969740-mRNA-1:exon:601;Parent=Msa0969740-mRNA-1
chr6_4	exon	1166542	1166856	1166542	ID=Msa0969740-mRNA-1:exon:602;Parent=Msa0969740-mRNA-1
chr6_4	CDS	1166140	1166145	1166140	ID=Msa0969740-mRNA-1:cds;Parent=Msa0969740-mRNA-1
chr6_4	CDS	1166542	1166856	1166542	ID=Msa0969740-mRNA-1:cds;Parent=Msa0969740-mRNA-1

Gene Sequence	>Msa0969740 ATGAGAAATACAAGGCCTAGTGCTGAAGATCTTCCTGGTGATTTAGCTATGATTGTAACATCATGTTGGAAAGAGGATCCAAATGATAGGCCGAATTTTAGTGAAATCATACAGATGCTCCTTCGGTATCTCTCTACCATTTCGCCATTAGAACCGGTTGTTCCAATGCGGATGGCTTCATCTGAGAATATTGTATTGCCACCAGAATCTCCCGGTACAAGTGCATTAATGTTTCGACGAGACGACTCTGGAGAAACCCCGAAAGCCAATGTGGAAGACAGGTTCAAAGGGTATTTCTTCTGCTTCAATCAGTGTTATTAA
Protein sequence	>Msa0969740 MRNTRPSAEDLPGDLAMIVTSCWKEDPNDRPNFSEIIQMLLRYLSTISPLEPVVPMRMASSENIVLPPESPGTSALMFRRDDSGETPKANVEDRFKGYFFCFNQCY*