AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0970300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970300 MtrunA17_Chr6g0449311 89.583 144 12 1 37 177 1 144 1.96e-75 224
Msa0970300 MtrunA17_Chr5g0439111 63.924 158 45 4 1 153 1 151 2.38e-59 187
Msa0970300 MtrunA17_Chr6g0452661 47.826 230 72 6 1 227 1 185 3.10e-58 184
Msa0970300 MtrunA17_Chr4g0055441 62.590 139 38 3 10 145 3 130 1.27e-52 167
Msa0970300 MtrunA17_Chr6g0452691 56.081 148 52 3 1 142 1 141 6.66e-52 166
Msa0970300 MtrunA17_Chr6g0452671 55.634 142 56 3 1 140 1 137 6.19e-46 152
Msa0970300 MtrunA17_Chr4g0059901 50.467 107 52 1 8 114 9 114 3.99e-31 115
Msa0970300 MtrunA17_Chr8g0342351 39.535 172 73 3 8 161 11 169 1.84e-29 110
Msa0970300 MtrunA17_Chr4g0058651 38.806 134 70 2 7 140 9 130 9.61e-26 99.4
Msa0970300 MtrunA17_Chr3g0097221 44.643 112 61 1 7 118 5 115 1.07e-25 101
Msa0970300 MtrunA17_Chr7g0227511 44.898 98 53 1 7 104 9 105 3.91e-25 97.4
Msa0970300 MtrunA17_Chr2g0330201 44.954 109 59 1 8 116 18 125 5.87e-25 100
Msa0970300 MtrunA17_Chr5g0402611 42.857 105 59 1 7 111 6 109 5.28e-24 97.8
Msa0970300 MtrunA17_Chr8g0353981 49.398 83 41 1 7 89 5 86 8.01e-24 95.5
Msa0970300 MtrunA17_Chr3g0115431 43.137 102 57 1 10 111 10 110 1.13e-23 94.7
Msa0970300 MtrunA17_Chr3g0117121 43.878 98 54 1 7 104 8 104 2.68e-23 93.2
Msa0970300 MtrunA17_Chr4g0043421 38.168 131 79 2 10 140 16 144 3.14e-22 89.7
Msa0970300 MtrunA17_Chr6g0449641 41.837 98 56 1 7 104 7 103 4.25e-22 88.6
Msa0970300 MtrunA17_Chr5g0444351 31.250 144 95 2 10 153 6 145 4.93e-22 88.6
Msa0970300 MtrunA17_Chr2g0311601 37.500 120 68 2 10 129 14 126 1.49e-21 87.8
Msa0970300 MtrunA17_Chr5g0437571 37.931 116 70 2 7 122 4 117 2.64e-21 87.0
Msa0970300 MtrunA17_Chr6g0449651 40.816 98 57 1 7 104 65 161 4.99e-21 87.4
Msa0970300 MtrunA17_Chr7g0225111 37.273 110 68 1 10 119 6 114 6.77e-21 84.7
Msa0970300 MtrunA17_Chr4g0030001 40.351 114 65 3 10 122 25 136 1.03e-20 86.3
Msa0970300 MtrunA17_Chr5g0437401 35.780 109 69 1 5 113 7 114 1.08e-20 85.9
Msa0970300 MtrunA17_Chr5g0438091 32.331 133 88 2 10 142 40 170 1.46e-20 85.9
Msa0970300 MtrunA17_Chr3g0106101 36.937 111 68 2 10 120 8 116 5.38e-20 84.0
Msa0970300 MtrunA17_Chr8g0374591 38.462 104 63 1 8 111 7 109 7.09e-20 82.4
Msa0970300 MtrunA17_Chr8g0374601 39.423 104 62 1 8 111 25 127 2.81e-19 84.3
Msa0970300 MtrunA17_Chr4g0040831 32.558 129 78 3 8 132 14 137 3.29e-19 82.4
Msa0970300 MtrunA17_Chr3g0114061 39.423 104 61 2 10 113 9 110 8.28e-19 80.5
Msa0970300 MtrunA17_Chr5g0432451 32.576 132 84 2 10 141 44 170 9.61e-19 82.0
Msa0970300 MtrunA17_Chr3g0113891 37.500 104 64 1 5 108 10 112 1.13e-18 80.9
Msa0970300 MtrunA17_Chr1g0200201 36.434 129 70 4 10 129 73 198 1.37e-18 81.6
Msa0970300 MtrunA17_Chr3g0129071 36.735 98 61 1 10 107 48 144 2.27e-18 80.9
Msa0970300 MtrunA17_Chr7g0260971 38.835 103 61 2 10 111 15 116 1.46e-16 75.9
Msa0970300 MtrunA17_Chr8g0351721 34.862 109 69 2 4 111 13 120 6.09e-15 71.2
Msa0970300 MtrunA17_Chr7g0245871 35.354 99 62 2 10 107 28 125 2.61e-14 70.1
Msa0970300 MtrunA17_Chr1g0184281 36.364 99 61 2 10 107 16 113 3.64e-14 68.9
Msa0970300 MtrunA17_Chr8g0351701 33.981 103 66 2 10 111 24 125 4.26e-14 69.3
Msa0970300 MtrunA17_Chr4g0046201 37.500 88 54 1 9 96 6 92 7.07e-14 69.3
Msa0970300 MtrunA17_Chr6g0460951 33.333 102 66 2 10 110 41 141 1.36e-13 67.8
Msa0970300 MtrunA17_Chr1g0184271 26.404 178 107 6 10 185 13 168 8.06e-13 65.9
Msa0970300 MtrunA17_Chr8g0374581 34.615 104 66 2 8 110 7 109 9.77e-13 65.5
Msa0970300 MtrunA17_Chr6g0460941 35.227 88 55 2 10 96 8 94 1.75e-12 61.6
Msa0970300 MtrunA17_Chr5g0435641 28.302 106 74 2 7 111 5 109 6.22e-12 62.8
Msa0970300 MtrunA17_Chr2g0326041 50.000 48 24 0 10 57 34 81 3.40e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970300 AT3G47870.1 55.769 104 45 1 9 112 36 138 3.04e-34 125
Msa0970300 AT3G13850.1 43.243 148 79 3 8 152 35 180 4.61e-30 113
Msa0970300 AT3G27650.1 42.424 99 56 1 7 105 37 134 2.75e-23 92.4
Msa0970300 AT5G63090.4 41.818 110 63 1 7 116 9 117 4.80e-23 92.8
Msa0970300 AT5G63090.3 41.818 110 63 1 7 116 9 117 4.80e-23 92.8
Msa0970300 AT5G63090.2 41.818 110 63 1 7 116 9 117 4.80e-23 92.8
Msa0970300 AT5G63090.1 41.818 110 63 1 7 116 9 117 4.80e-23 92.8
Msa0970300 AT1G65620.1 46.988 83 43 1 7 89 7 88 2.02e-22 91.3
Msa0970300 AT1G65620.4 46.988 83 43 1 7 89 7 88 2.02e-22 91.3
Msa0970300 AT1G65620.3 46.988 83 43 1 7 89 7 88 2.02e-22 91.3
Msa0970300 AT1G65620.5 46.988 83 43 1 7 89 7 88 2.02e-22 91.3
Msa0970300 AT1G65620.2 46.988 83 43 1 7 89 7 88 2.02e-22 91.3
Msa0970300 AT3G26660.1 39.130 115 67 2 8 120 4 117 2.12e-22 89.0
Msa0970300 AT5G66870.1 41.176 102 59 1 10 111 8 108 8.39e-22 92.0
Msa0970300 AT1G31320.1 45.263 95 48 2 5 96 6 99 2.35e-21 87.8
Msa0970300 AT3G26620.1 38.393 112 66 2 8 117 4 114 5.39e-21 85.5
Msa0970300 AT1G72980.1 41.071 112 64 2 9 119 13 123 1.10e-20 87.0
Msa0970300 AT1G07900.1 41.489 94 54 1 10 103 34 126 1.38e-19 83.6
Msa0970300 AT2G30130.1 40.385 104 60 2 10 113 9 110 9.03e-19 81.6
Msa0970300 AT2G28500.1 37.391 115 70 2 10 124 56 168 1.39e-18 82.0
Msa0970300 AT2G30340.2 38.776 98 59 1 10 107 54 150 1.56e-18 82.4
Msa0970300 AT2G30340.1 38.776 98 59 1 10 107 53 149 1.88e-18 82.0
Msa0970300 AT2G40470.2 33.813 139 82 4 10 148 21 149 4.27e-18 79.7
Msa0970300 AT2G40470.1 33.813 139 82 4 10 148 46 174 6.78e-18 79.7
Msa0970300 AT2G23660.2 40.594 101 59 1 10 110 6 105 7.61e-18 81.3
Msa0970300 AT2G23660.3 40.594 101 59 1 10 110 6 105 7.61e-18 81.3
Msa0970300 AT2G23660.1 40.594 101 59 1 10 110 6 105 7.61e-18 81.3
Msa0970300 AT3G11090.1 35.354 99 63 1 5 103 7 104 8.33e-18 78.2
Msa0970300 AT1G16530.1 36.283 113 63 3 5 109 10 121 1.49e-17 77.4
Msa0970300 AT5G15060.1 45.946 74 39 1 10 82 18 91 3.53e-17 75.9
Msa0970300 AT1G06280.1 38.298 94 53 3 8 101 23 111 1.58e-15 73.2
Msa0970300 AT2G19820.1 34.343 99 62 1 1 96 1 99 2.52e-15 70.5
Msa0970300 AT3G50510.1 37.143 105 64 2 10 113 13 116 2.60e-15 72.4
Msa0970300 AT3G50510.2 37.143 105 64 2 10 113 13 116 2.60e-15 72.4
Msa0970300 AT2G42430.1 28.966 145 101 2 10 153 16 159 3.74e-15 72.8
Msa0970300 AT4G00210.1 33.333 138 84 4 10 145 12 143 6.16e-15 71.6
Msa0970300 AT4G00220.1 34.286 105 67 2 12 115 20 123 7.14e-14 68.9
Msa0970300 AT3G58190.1 35.000 100 63 2 10 108 12 110 2.55e-13 67.4
Msa0970300 AT2G42440.1 33.000 100 65 2 10 108 8 106 2.78e-13 67.8
Msa0970300 AT2G45420.1 33.333 105 68 2 12 115 40 143 5.64e-13 67.0
Msa0970300 AT3G03760.1 34.314 102 65 2 10 110 52 152 1.90e-12 65.5
Msa0970300 AT5G35900.1 28.431 102 72 1 10 111 6 106 7.54e-12 63.2
Msa0970300 AT2G31310.1 28.689 122 77 3 10 122 8 128 1.06e-11 62.4
Msa0970300 AT2G45410.1 35.644 101 63 2 12 111 19 118 1.94e-11 61.6

Find 27 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCAACATTGCTAGATAT+TGG 0.233878 6_4:-1770674 Msa0970290:intron
TCACCAACATTGTTAAATAT+TGG 0.234385 6_4:-1770501 Msa0970290:intron
CTTTCTTCTTTGATATTTGC+AGG 0.243555 6_4:-1770118 Msa0970290:intron
ATTATTATGTGATAATAATA+AGG 0.272056 6_4:-1770528 Msa0970290:intron
TGGATGTCTTGGTCTTATTA+TGG 0.281895 6_4:+1770332 Msa0970300:CDS
GGTTTATCGGCTGGAAAATA+AGG 0.296290 6_4:-1770165 Msa0970290:intron
ATAATGAATACTCAAGTTCT+TGG 0.299220 6_4:+1770546 Msa0970300:CDS
GCCAATGCTCATCGTTTGTT+TGG 0.347008 6_4:+1770198 Msa0970300:CDS
TAAGGAGCTAAAGGGAAATC+TGG 0.373984 6_4:-1770147 Msa0970290:intron
ATTCCAATATTTAACAATGT+TGG 0.448330 6_4:+1770498 Msa0970300:CDS
TCTTCTACATTTCTCTTCAT+CGG 0.464845 6_4:+1770443 Msa0970300:CDS
GGAATTGAAGAACTAGTAGA+AGG 0.471090 6_4:-1770480 Msa0970290:intron
TTCCCAAGTCATGGATGTCT+TGG 0.482571 6_4:+1770321 Msa0970300:CDS
GCTGGAAAATAAGGAGCTAA+AGG 0.488184 6_4:-1770156 Msa0970290:intron
CTGGAAAATAAGGAGCTAAA+GGG 0.491954 6_4:-1770155 Msa0970290:intron
GAATGATCCATTGAAAAGTA+AGG 0.496518 6_4:-1770624 Msa0970290:intron
AATCCAATATCTAGCAATGT+TGG 0.505027 6_4:+1770671 Msa0970300:CDS
ATGGATACAACAAGGGTACA+TGG 0.514070 6_4:+1770756 Msa0970300:CDS
AGACCAAGACATCCATGACT+TGG 0.541471 6_4:-1770324 Msa0970290:intron
ACCAAACAAACGATGAGCAT+TGG 0.553402 6_4:-1770199 Msa0970290:intron
CGTGCAAGATTCCCAAGTCA+TGG 0.565788 6_4:+1770312 Msa0970300:CDS
TACATGGTTGCACAACTGAT+TGG 0.568443 6_4:+1770772 Msa0970300:CDS
GTAAATTTGTTAACATCGTG+AGG 0.570877 6_4:-1770705 Msa0970290:intron
GACCAAGACATCCATGACTT+GGG 0.599785 6_4:-1770323 Msa0970290:intron
ATATTGGATTTGAGAACTAG+TGG 0.602290 6_4:-1770658 Msa0970290:intron
TTTGTTTGGTATTTCCAACA+TGG 0.612102 6_4:+1770212 Msa0970300:CDS
TGGATTTGAGAACTAGTGGT+AGG 0.612330 6_4:-1770654 Msa0970290:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATGTGATAATAATA+AGG - chr6_4:1770531-1770550 Msa0970290:intron 10.0%
!!! AAATTCGAATATAAATTTTA+TGG + chr6_4:1770737-1770756 Msa0970300:CDS 10.0%
!! ATTCCAATATTTAACAATGT+TGG + chr6_4:1770498-1770517 Msa0970300:CDS 20.0%
!!! TAAATTTTATGGATACAACA+AGG + chr6_4:1770748-1770767 Msa0970300:CDS 20.0%
!!! AAATTTTATGGATACAACAA+GGG + chr6_4:1770749-1770768 Msa0970300:CDS 20.0%
!!! GAAGTCAATTATTTTTGAGT+CGG + chr6_4:1770284-1770303 Msa0970300:CDS 25.0%
! TCACCAACATTGTTAAATAT+TGG - chr6_4:1770504-1770523 Msa0970290:intron 25.0%
! ATAATGAATACTCAAGTTCT+TGG + chr6_4:1770546-1770565 Msa0970300:CDS 25.0%
!!! TAATCTACCTTACTTTTCAA+TGG + chr6_4:1770617-1770636 Msa0970300:CDS 25.0%
GGATATTCACAGAAAAAACA+AGG + chr6_4:1770086-1770105 Msa0970300:CDS 30.0%
CTTTCTTCTTTGATATTTGC+AGG - chr6_4:1770121-1770140 Msa0970290:intron 30.0%
TTTGTTTGGTATTTCCAACA+TGG + chr6_4:1770212-1770231 Msa0970300:CDS 30.0%
TCTTCTACATTTCTCTTCAT+CGG + chr6_4:1770443-1770462 Msa0970300:CDS 30.0%
GAATGATCCATTGAAAAGTA+AGG - chr6_4:1770627-1770646 Msa0970290:intron 30.0%
ATATTGGATTTGAGAACTAG+TGG - chr6_4:1770661-1770680 Msa0970290:intron 30.0%
AATCCAATATCTAGCAATGT+TGG + chr6_4:1770671-1770690 Msa0970300:CDS 30.0%
TTACCAACATTGCTAGATAT+TGG - chr6_4:1770677-1770696 Msa0970290:intron 30.0%
!! GTAAATTTGTTAACATCGTG+AGG - chr6_4:1770708-1770727 Msa0970290:intron 30.0%
CTGGAAAATAAGGAGCTAAA+GGG - chr6_4:1770158-1770177 Msa0970290:intron 35.0%
! TTTTAGCATCTTCACCATGT+TGG - chr6_4:1770229-1770248 Msa0970290:intron 35.0%
!! TGGATGTCTTGGTCTTATTA+TGG + chr6_4:1770332-1770351 Msa0970300:CDS 35.0%
GGAATTGAAGAACTAGTAGA+AGG - chr6_4:1770483-1770502 Msa0970290:intron 35.0%
TAAGGAGCTAAAGGGAAATC+TGG - chr6_4:1770150-1770169 Msa0970290:intron 40.0%
GCTGGAAAATAAGGAGCTAA+AGG - chr6_4:1770159-1770178 Msa0970290:intron 40.0%
! GGTTTATCGGCTGGAAAATA+AGG - chr6_4:1770168-1770187 Msa0970290:intron 40.0%
!!! AGCATTTTTGGTTTATCGGC+TGG - chr6_4:1770177-1770196 Msa0970290:intron 40.0%
!!! GGCGAGCATTTTTGGTTTAT+CGG - chr6_4:1770181-1770200 Msa0970290:intron 40.0%
ACCAAACAAACGATGAGCAT+TGG - chr6_4:1770202-1770221 Msa0970290:intron 40.0%
TGGATTTGAGAACTAGTGGT+AGG - chr6_4:1770657-1770676 Msa0970290:intron 40.0%
! ATGGATACAACAAGGGTACA+TGG + chr6_4:1770756-1770775 Msa0970300:CDS 40.0%
TACATGGTTGCACAACTGAT+TGG + chr6_4:1770772-1770791 Msa0970300:CDS 40.0%
!!! TGAGCATTGGCGAGCATTTT+TGG - chr6_4:1770189-1770208 Msa0970290:intron 45.0%
GCCAATGCTCATCGTTTGTT+TGG + chr6_4:1770198-1770217 Msa0970300:CDS 45.0%
TTCCCAAGTCATGGATGTCT+TGG + chr6_4:1770321-1770340 Msa0970300:CDS 45.0%
GACCAAGACATCCATGACTT+GGG - chr6_4:1770326-1770345 Msa0970290:intron 45.0%
AGACCAAGACATCCATGACT+TGG - chr6_4:1770327-1770346 Msa0970290:intron 45.0%
CGTGCAAGATTCCCAAGTCA+TGG + chr6_4:1770312-1770331 Msa0970300:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 1770084 1770797 1770084 ID=Msa0970300;Name=Msa0970300
chr6_4 mRNA 1770084 1770797 1770084 ID=Msa0970300-mRNA-1;Parent=Msa0970300;Name=Msa0970300-mRNA-1;_AED=0.02;_eAED=0.10;_QI=0|-1|0|1|-1|1|1|0|237
chr6_4 exon 1770084 1770797 1770084 ID=Msa0970300-mRNA-1:exon:845;Parent=Msa0970300-mRNA-1
chr6_4 CDS 1770084 1770797 1770084 ID=Msa0970300-mRNA-1:cds;Parent=Msa0970300-mRNA-1
Gene Sequence

>Msa0970300

ATGGATATTCACAGAAAAAACAAGGCATGTGCAGCCTGCAAATATCAAAGAAGAAAGTGTACTCCAGATTTCCCTTTAGCTCCTTATTTTCCAGCCGATAAACCAAAAATGCTCGCCAATGCTCATCGTTTGTTTGGTATTTCCAACATGGTGAAGATGCTAAAATCTATTGACGATGATGATCATAAAGATGAAGCAATGAAGTCAATTATTTTTGAGTCGGAAATGCGTGCAAGATTCCCAAGTCATGGATGTCTTGGTCTTATTATGGAGTATCAAGATATGATAATTGAGTCTATGAAAGAGTTAGATCATGTAAAACAGTTACTAAGTTTGTGTAAGCTATCACAACAACAAAATCTTCTACATTTCTCTTCATCGGATCATTCTTCAGTACCTTCTACTAGTTCTTCAATTCCAATATTTAACAATGTTGGTGATGTTCCTTATTATTATCACATAATAATGAATACTCAAGTTCTTGGAAATCTTGATATGATAAAGTATGAAGAAGAGTTAGATTATGTAAAAAATAATCTACCTTACTTTTCAATGGATCATTCTTCTGTACCTACCACTAGTTCTCAAATCCAATATCTAGCAATGTTGGTAACATTCATTCCTCACGATGTTAACAAATTTACATCATATACAAATTCGAATATAAATTTTATGGATACAACAAGGGTACATGGTTGCACAACTGATTGGTGA

Protein sequence

>Msa0970300

MDIHRKNKACAACKYQRRKCTPDFPLAPYFPADKPKMLANAHRLFGISNMVKMLKSIDDDDHKDEAMKSIIFESEMRARFPSHGCLGLIMEYQDMIIESMKELDHVKQLLSLCKLSQQQNLLHFSSSDHSSVPSTSSSIPIFNNVGDVPYYYHIIMNTQVLGNLDMIKYEEELDYVKNNLPYFSMDHSSVPTTSSQIQYLAMLVTFIPHDVNKFTSYTNSNINFMDTTRVHGCTTDW*