AlfalfaGEDB | Msa0974920

M. sativa cultivar zhongmu-4 / Msa0974920

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974920	KAG4968099.1	65.000	60	21	0	1	60	1	60	3.22e-19	85.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974920	sp\|O81833\|SD11_ARATH	54.386	57	26	0	1	57	669	725	3.32e-13	65.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974920	A0A396HC12	66.667	60	20	0	1	60	37	96	5.11e-19	85.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974920	MtrunA17_Chr6g0453561	66.667	60	20	1	60	37	96	9.83e-23	85.1
Msa0974920	MtrunA17_Chr3g0084631	65.306	49	17	1	49	33	81	1.12e-17	72.0
Msa0974920	MtrunA17_Chr3g0084681	67.347	49	16	1	49	634	682	2.52e-17	73.6
Msa0974920	MtrunA17_Chr3g0098781	65.306	49	17	1	49	81	129	2.76e-17	72.0
Msa0974920	MtrunA17_Chr3g0084431	53.226	62	29	1	62	664	725	7.29e-17	72.4
Msa0974920	MtrunA17_Chr3g0084421	63.265	49	18	1	49	632	680	7.58e-17	72.4
Msa0974920	MtrunA17_Chr3g0084661	54.839	62	28	1	62	203	264	1.04e-16	71.6
Msa0974920	MtrunA17_Chr3g0084411	63.265	49	18	1	49	669	717	6.18e-16	69.7
Msa0974920	MtrunA17_Chr3g0084691	63.265	49	18	1	49	692	740	1.02e-15	68.9
Msa0974920	MtrunA17_Chr3g0084491	51.613	62	30	1	62	676	737	6.06e-15	67.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0974920	AT4G27300.1	54.386	57	26	0	1	57	669	725	3.38e-14	65.1
Msa0974920	AT4G21366.1	59.184	49	20	0	1	49	1	49	3.97e-14	61.6
Msa0974920	AT4G27290.1	55.102	49	22	0	1	49	634	682	2.17e-12	60.1
Msa0974920	AT4G27290.2	55.102	49	22	0	1	49	680	728	2.26e-12	60.1
Msa0974920	AT1G61360.2	55.102	49	22	0	1	49	586	634	3.23e-12	59.7
Msa0974920	AT1G61360.3	55.102	49	22	0	1	49	586	634	3.23e-12	59.7
Msa0974920	AT1G61360.4	55.102	49	22	0	1	49	586	634	3.23e-12	59.7
Msa0974920	AT1G61360.1	55.102	49	22	0	1	49	667	715	3.40e-12	59.7
Msa0974920	AT4G23200.1	54.000	50	20	2	1	48	505	553	1.84e-11	57.4
Msa0974920	AT1G65790.1	52.083	48	23	0	1	48	688	735	2.12e-11	57.4

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Find 7 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAGATGTCTTTAATTA+TGG	0.234358	6_4:-8462087	Msa0974920:CDS
ACATGCCTCCAGAGTATGCT+AGG	0.542978	6_4:-8462128	Msa0974920:CDS
ATTGTACTAGAGATTGTGTT+CGG	0.581542	6_4:-8462060	Msa0974920:CDS
CCACTCCTAGCATACTCTGG+AGG	0.698493	6_4:+8462123	None:intergenic
CCTCCAGAGTATGCTAGGAG+TGG	0.626998	6_4:-8462123	Msa0974920:CDS
GAATGTAAGAGCAAAGTTTG+TGG	0.459228	6_4:-8461971	Msa0974920:CDS
TGTCCACTCCTAGCATACTC+TGG	0.561698	6_4:+8462120	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTGTACTAGAGATTGTGTT+CGG	-	chr6_4:8462026-8462045	Msa0974920:CDS	30.0%
	GAATGTAAGAGCAAAGTTTG+TGG	-	chr6_4:8462115-8462134	Msa0974920:CDS	35.0%
	TGTCCACTCCTAGCATACTC+TGG	+	chr6_4:8461969-8461988	None:intergenic	50.0%
	CCACTCCTAGCATACTCTGG+AGG	+	chr6_4:8461966-8461985	None:intergenic	55.0%
	CCTCCAGAGTATGCTAGGAG+TGG	-	chr6_4:8461963-8461982	Msa0974920:CDS	55.0%
!!	AAATGTAACAAAAATATTCT+TGG	-	chr6_4:8462077-8462096	Msa0974920:CDS	15.0%
!!	AAATCAGATGTCTTTAATTA+TGG	-	chr6_4:8461999-8462018	Msa0974920:CDS	20.0%
!!!	GAATATTTTTGTTACATTTA+GGG	+	chr6_4:8462076-8462095	None:intergenic	15.0%
!!!	AGAATATTTTTGTTACATTT+AGG	+	chr6_4:8462077-8462096	None:intergenic	15.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	8461960	8462148	8461960	ID=Msa0974920;Name=Msa0974920
chr6_4	mRNA	8461960	8462148	8461960	ID=Msa0974920-mRNA-1;Parent=Msa0974920;Name=Msa0974920-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|62
chr6_4	exon	8461960	8462148	8461960	ID=Msa0974920-mRNA-1:exon:3096;Parent=Msa0974920-mRNA-1
chr6_4	CDS	8461960	8462148	8461960	ID=Msa0974920-mRNA-1:cds;Parent=Msa0974920-mRNA-1

Gene Sequence	>Msa0974920 ATGCCTCCAGAGTATGCTAGGAGTGGACATTTCTCTACGAAATCAGATGTCTTTAATTATGGTGTGATTGTACTAGAGATTGTGTTCGGAAAGAAGAATAGAGAATTTTCAGACCCTAAATGTAACAAAAATATTCTTGGATATGCAAGTATTATGAATGTAAGAGCAAAGTTTGTGGAAAGTATCTAA
Protein sequence	>Msa0974920 MPPEYARSGHFSTKSDVFNYGVIVLEIVFGKKNREFSDPKCNKNILGYASIMNVRAKFVESI*