AlfalfaGEDB | Msa0977960

M. sativa cultivar zhongmu-4 / Msa0977960

KEGG: K04498
GO: -
PFAM: PF00628.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977960	RHN50388.1	91.271	779	64	3	1	778	1	776	0.0	1465

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977960	sp\|Q6YXY2\|HACL1_ORYSJ	47.724	769	348	13	46	775	904	1657	0.0	719

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977960	A0A396HCL0	91.271	779	64	3	1	778	1	776	0.0	1465

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TFs/TRs:

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Gene ID	Type	Classification
Msa0977960	TR	TAZ

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977960	MtrunA17_Chr6g0456861	91.271	779	64	3	1	778	1	776	0.0	1465
Msa0977960	MtrunA17_Chr5g0438991	83.983	718	100	7	58	765	246	958	0.0	1231
Msa0977960	MtrunA17_Chr3g0138411	70.889	821	173	11	22	778	388	1206	0.0	1169
Msa0977960	MtrunA17_Chr3g0133461	67.832	858	185	14	2	778	496	1343	0.0	1133
Msa0977960	MtrunA17_Chr4g0000031	46.617	798	336	15	55	775	913	1697	0.0	708
Msa0977960	MtrunA17_Chr6g0456851	63.747	491	152	9	59	529	349	833	0.0	611
Msa0977960	MtrunA17_Chr6g0456851	73.770	61	15	1	584	644	820	879	8.04e-19	91.7
Msa0977960	MtrunA17_Chr6g0456931	71.020	245	63	2	251	487	1	245	7.69e-120	360
Msa0977960	MtrunA17_Chr5g0401991	77.056	231	41	3	472	700	1	221	1.66e-114	345
Msa0977960	MtrunA17_Chr5g0402001	85.149	101	14	1	322	421	1	101	3.79e-53	179

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0977960	AT1G79000.1	46.106	796	345	14	54	775	900	1685	717
Msa0977960	AT1G79000.2	46.106	796	345	14	54	775	900	1685	717
Msa0977960	AT1G79000.3	46.106	796	345	14	54	775	876	1661	716
Msa0977960	AT1G16710.2	46.532	793	345	16	55	775	880	1665	702
Msa0977960	AT1G16710.3	46.532	793	345	16	55	775	883	1668	702
Msa0977960	AT1G16710.10	46.532	793	345	16	55	775	883	1668	702
Msa0977960	AT1G16710.8	46.532	793	345	16	55	775	883	1668	702
Msa0977960	AT1G16710.7	46.532	793	345	16	55	775	883	1668	702
Msa0977960	AT1G16710.6	46.532	793	345	16	55	775	844	1629	702
Msa0977960	AT1G16710.4	46.532	793	345	16	55	775	844	1629	702

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Find 119 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGAGAAGATGCTTGCCA+AGG	0.601647	6_4:+12864763	None:intergenic
AAAGAAATGATCATACACGT+TGG	0.679280	6_4:-12866918	None:intergenic
AAATAGAACAGTAAAGGACA+TGG	0.650828	6_4:+12867090	Msa0977960:CDS
AACGAGAAGATGCTTGCCAA+GGG	0.580333	6_4:+12864764	None:intergenic
AAGTAGGATTCTCCAAGGAC+TGG	0.543459	6_4:+12864852	Msa0977960:CDS
AATAGAACAGTAAAGGACAT+GGG	0.660656	6_4:+12867091	Msa0977960:CDS
AATATCGCAAGTGAGCAACG+AGG	0.784334	6_4:-12867277	None:intergenic
AATGAAGAAAATATTGTCGT+TGG	0.489418	6_4:+12866890	Msa0977960:CDS
AATGATATCGTCCTTGAGAG+TGG	0.608339	6_4:+12867651	Msa0977960:CDS
AATGCAATAAATGCAAAAGA+TGG	0.545817	6_4:+12865689	Msa0977960:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATGAATTAAGCAATCAAGT+AGG	+	chr6_4:12864836-12864855	Msa0977960:CDS	30.0%
ACATATTAGTAGTCTGAGAA+AGG	+	chr6_4:12864988-12865007	Msa0977960:CDS	30.0%
ATCCAAGGATTAACAAATCA+AGG	-	chr6_4:12865057-12865076	None:intergenic	30.0%
TTTAACACAGATTACTTCTG+AGG	+	chr6_4:12865292-12865311	Msa0977960:intron	30.0%
CCACAACATAAACAAAAAAG+TGG	-	chr6_4:12865386-12865405	None:intergenic	30.0%
GTGAAGATGTCAAAATCAAT+GGG	+	chr6_4:12865498-12865517	Msa0977960:CDS	30.0%
TGATGAAGTAATTGAAGAAC+CGG	+	chr6_4:12865559-12865578	Msa0977960:CDS	30.0%
CTATAACAAGAAAGCAGATT+TGG	+	chr6_4:12865732-12865751	Msa0977960:CDS	30.0%
AAAAAACTGGGAAAAAGATG+AGG	+	chr6_4:12865915-12865934	Msa0977960:CDS	30.0%
GATCTATAAGAGAATTCAGT+AGG	-	chr6_4:12866178-12866197	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	12864773	12868579	12864773	ID=Msa0977960;Name=Msa0977960
chr6_4	mRNA	12864773	12868579	12864773	ID=Msa0977960-mRNA-1;Parent=Msa0977960;Name=Msa0977960-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0.5\|0.46\|1\|0.91\|0.84\|13\|86\|778
chr6_4	exon	12864773	12865021	12864773	ID=Msa0977960-mRNA-1:exon:4451;Parent=Msa0977960-mRNA-1
chr6_4	exon	12865302	12865580	12865302	ID=Msa0977960-mRNA-1:exon:4452;Parent=Msa0977960-mRNA-1
chr6_4	exon	12865682	12865936	12865682	ID=Msa0977960-mRNA-1:exon:4453;Parent=Msa0977960-mRNA-1
chr6_4	exon	12866073	12866201	12866073	ID=Msa0977960-mRNA-1:exon:4454;Parent=Msa0977960-mRNA-1
chr6_4	exon	12866281	12866481	12866281	ID=Msa0977960-mRNA-1:exon:4455;Parent=Msa0977960-mRNA-1
chr6_4	exon	12866588	12866730	12866588	ID=Msa0977960-mRNA-1:exon:4456;Parent=Msa0977960-mRNA-1
chr6_4	exon	12866865	12867153	12866865	ID=Msa0977960-mRNA-1:exon:4457;Parent=Msa0977960-mRNA-1
chr6_4	exon	12867242	12867381	12867242	ID=Msa0977960-mRNA-1:exon:4458;Parent=Msa0977960-mRNA-1
chr6_4	exon	12867471	12867528	12867471	ID=Msa0977960-mRNA-1:exon:4459;Parent=Msa0977960-mRNA-1
chr6_4	exon	12867615	12867887	12867615	ID=Msa0977960-mRNA-1:exon:4460;Parent=Msa0977960-mRNA-1
chr6_4	exon	12867981	12868213	12867981	ID=Msa0977960-mRNA-1:exon:4461;Parent=Msa0977960-mRNA-1
chr6_4	exon	12868382	12868502	12868382	ID=Msa0977960-mRNA-1:exon:4462;Parent=Msa0977960-mRNA-1
chr6_4	exon	12868527	12868579	12868527	ID=Msa0977960-mRNA-1:exon:4463;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12864773	12865021	12864773	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12865302	12865580	12865302	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12865682	12865936	12865682	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12866073	12866201	12866073	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12866281	12866481	12866281	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12866588	12866730	12866588	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12866865	12867153	12866865	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12867242	12867381	12867242	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12867471	12867528	12867471	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12867615	12867887	12867615	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12867981	12868213	12867981	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	CDS	12868382	12868469	12868382	ID=Msa0977960-mRNA-1:cds;Parent=Msa0977960-mRNA-1
chr6_4	three_prime_UTR	12868470	12868502	12868470	ID=Msa0977960-mRNA-1:three_prime_utr;Parent=Msa0977960-mRNA-1
chr6_4	three_prime_UTR	12868527	12868579	12868527	ID=Msa0977960-mRNA-1:three_prime_utr;Parent=Msa0977960-mRNA-1

Gene Sequence

>Msa0977960

ATGCTTGCCAAGGGTGGGAATACTTCATCAGATGTCTCAAACATCTCGAGAAAAAGATGTTTTGATGAATTAAGCAATCAAGTAGGATTCTCCAAGGACTGGAGGTATCATCCTGAAATGGTACGCAAACGTTATGATCAATCGATTCACAATACAATTCCCCCAAAAGTTGCTGTTTCCTTGATTGATTCGTTCAGACGTGACCAAATAGAAGAACATATTAGTAGTCTGAGAAAGGAATCTGATCAGATTACTTCTGAGGATCAAGCGGGAATTGATGCAAACACTTGCAACCTATGTAAAAGGGAAAGACTTTACTTTGCTAAAGTCCCACTTTTTTGTTTATGTTGTGGTGTTCGCATCAAGAAAATATATTTTTGTACAAAAGAAGAAGAATTTGTTGCACAAGGTTGCATTTGCTCTGAGTGTTACAAGACTTTTAAAGGTGAAGATGTCAAAATCAATGGGACATTTATTTCCAAGAGAAATCTTGCAAAGAGGAATAATGATGAAGTAATTGAAGAACCGTGGGTTGAATGCAATAAATGCAAAAGATGGCAGCATCAAATATGTGCACTCTATAACAAGAAAGCAGATTTGGACTGCAGTGCTGAGTATATATGTCCTTTATGCCGCTTAAAAGAAATTGAAAATAGAATGCATGTTCCAAAAGCTGCTAACTTTGGTGCCAAGGACTTGACAAGAACTGTGCTAAGTGACCACTTAGAGAAAAGACTTTTCAAGCGTCTCATGCAAGAGAGAAAAAACTGGGAAAAAGATGAGGTTTTAGCAGCAGAAAGTCTTTCAATTAGAGAAGTGCTATCTGTCGACAAGCAATTGAAAGTGAATAAGCAGTTTCTGGACATCATCCCAGAGGAGAATTACCCTACTGAATTCTCTTATAGATCAAGGGTTATACTTCTGTTTCAGAAAATTGAAGGAGTAGATGTATGCATTTTTGGAATGTATGTCCAGGAGTTTGGCTCGGAATGTGGCACTCCGAATCAGCGATGTGTATACATTTCATATCTTGATTCCGTCAAGTATTTCAGGCCTAAAAGAAGGACAAAGAGTGGAGAAGCCCTTCGTACCTTTGTTTACCATGAGATATTGATTGGATATCTTGATTTTTGTAAGGAAAGGGGTTTCTCAACTTGCTACATATGGGCCTGTCCTCCAAAAAAAGGTGATGATTATATACTATATTGCCATCCCAAAGAGCAAAAGACTCCGAAGAAAGATAAGCTACGCCACTGGTATCTTTCAATGCTAAAAAAGGCTAATGAAGAAAATATTGTCGTTGGTTTAACCAACGTGTATGATCATTTCTTTGTTCCTACTGAAAAAAGGAAATCCAAGATGACAGTTTCTCGTTTGCCATACTTTGATGGAGACTGGTGGTGCGGTAATGCTATGGTAGTTGCGAAGACCCTCGAAAAAGAGAGTAGAGCAGACTATGAAAAGTTGTTGAAACAAGTGTCAAATAGAACAGTAAAGGACATGGGACATGCCAAGCCTTCCAAAGACATACTGGTGATGCAAAAAGTTGGCCAAAATATATTGCCTACAAGAGAAAACTTCCTCGTTGCTCACTTGCGATATTCTTGCATGCACTGCCGTGAAGTGATAGTTTCTGGGAAGCGTTGGTTTTGCACTGAATGCAAGAATTTTCAGGAGTGTGAAAGATGCTATAGTTCTGAAAATCACACTTTAAAGAACGGCGAGGTGCATACACTTTGTCAGGCTAATGTTGATGATATCCCTTCCGACACTAAATGTAATGATATCGTCCTTGAGAGTGGATTGTTTGAAAATAGGGACAACTTTTTGATCTTCTGCCAGAAGTCTCAGTTACAGTTTGATACACTCCGTCGTGCCAAGTATTCCTCCATGATGATCCTCTACCATCTACACAATCCTAATGTGTTGACTCAAAATGAACACCAGTTGACTCAAAATTGTAATCGTTCAACACCAGTATATCAATCTGGGAATCAGGAGTCAAATGAGAAAATGATGGTGAAACTGATGAACGTTTTGAAGCATGCATCTCAATGCCGCCGCCGCGCAAGTAATGCTGAGCCATGCTCTCATCCAAACTGTTCTCAAATCAAGAAGCTGTTCTATCATGCCAGTAAGTGTGAAATTCGAGCTAATCGAGGCTGCCAGCATTGTAAAAAGATATGGTTTATACTAACTGTTCACTCCAGAAATTGCAAAGACTCGGAATGTAGAATACCACGCTGCAGTGATCTAAAGAAGCATTTAGAAAAGAGATCAAAGCAGTCTGAATCTTGCCGTAGAGCTGAAGTTTTGGAAGCAGTCATTGGCCAATGA

Protein sequence

>Msa0977960

MLAKGGNTSSDVSNISRKRCFDELSNQVGFSKDWRYHPEMVRKRYDQSIHNTIPPKVAVSLIDSFRRDQIEEHISSLRKESDQITSEDQAGIDANTCNLCKRERLYFAKVPLFCLCCGVRIKKIYFCTKEEEFVAQGCICSECYKTFKGEDVKINGTFISKRNLAKRNNDEVIEEPWVECNKCKRWQHQICALYNKKADLDCSAEYICPLCRLKEIENRMHVPKAANFGAKDLTRTVLSDHLEKRLFKRLMQERKNWEKDEVLAAESLSIREVLSVDKQLKVNKQFLDIIPEENYPTEFSYRSRVILLFQKIEGVDVCIFGMYVQEFGSECGTPNQRCVYISYLDSVKYFRPKRRTKSGEALRTFVYHEILIGYLDFCKERGFSTCYIWACPPKKGDDYILYCHPKEQKTPKKDKLRHWYLSMLKKANEENIVVGLTNVYDHFFVPTEKRKSKMTVSRLPYFDGDWWCGNAMVVAKTLEKESRADYEKLLKQVSNRTVKDMGHAKPSKDILVMQKVGQNILPTRENFLVAHLRYSCMHCREVIVSGKRWFCTECKNFQECERCYSSENHTLKNGEVHTLCQANVDDIPSDTKCNDIVLESGLFENRDNFLIFCQKSQLQFDTLRRAKYSSMMILYHLHNPNVLTQNEHQLTQNCNRSTPVYQSGNQESNEKMMVKLMNVLKHASQCRRRASNAEPCSHPNCSQIKKLFYHASKCEIRANRGCQHCKKIWFILTVHSRNCKDSECRIPRCSDLKKHLEKRSKQSESCRRAEVLEAVIGQ*