AlfalfaGEDB | Msa0977970

M. sativa cultivar zhongmu-4 / Msa0977970

KEGG: K04498
GO: -
PFAM: PF08214.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977970	RHN50388.1	82.368	777	81	9	3	724	1	776	0.0	1266

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977970	sp\|Q6YXY2\|HACL1_ORYSJ	45.778	758	318	14	45	717	904	1653	0.0	658

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977970	A0A396HCL0	82.368	777	81	9	3	724	1	776	0.0	1266

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TFs/TRs:

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Gene ID	Type	Classification
Msa0977970	TR	TAZ

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0977970	MtrunA17_Chr6g0456861	82.368	777	81	9	3	724	1	776	0.0	1266
Msa0977970	MtrunA17_Chr5g0438991	75.385	715	114	10	57	711	246	958	0.0	1061
Msa0977970	MtrunA17_Chr3g0138411	64.508	803	167	14	38	724	406	1206	0.0	999
Msa0977970	MtrunA17_Chr3g0133461	63.442	796	156	13	57	724	555	1343	0.0	963
Msa0977970	MtrunA17_Chr4g0000031	43.829	794	319	13	50	721	909	1697	0.0	640
Msa0977970	MtrunA17_Chr6g0456851	55.440	579	168	13	57	598	348	873	0.0	582
Msa0977970	MtrunA17_Chr6g0456931	72.034	236	58	2	226	453	1	236	9.72e-118	353
Msa0977970	MtrunA17_Chr5g0401991	79.878	164	20	1	496	646	58	221	9.45e-86	270
Msa0977970	MtrunA17_Chr5g0402001	84.158	101	15	1	297	396	1	101	6.81e-52	175
Msa0977970	MtrunA17_Chr6g0456921	50.000	156	20	4	297	451	1	99	7.23e-35	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0977970	AT1G79000.1	43.899	795	314	16	50	719	896	1683	645
Msa0977970	AT1G79000.3	43.899	795	314	16	50	719	872	1659	645
Msa0977970	AT1G79000.2	43.789	797	316	16	50	721	896	1685	644
Msa0977970	AT1G16710.1	43.270	795	323	15	50	721	905	1694	630
Msa0977970	AT1G16710.3	43.380	793	321	15	50	719	879	1666	629
Msa0977970	AT1G16710.6	43.270	795	323	15	50	721	840	1629	629
Msa0977970	AT1G16710.10	43.380	793	321	15	50	719	879	1666	629
Msa0977970	AT1G16710.8	43.380	793	321	15	50	719	879	1666	629
Msa0977970	AT1G16710.4	43.270	795	323	15	50	721	840	1629	629
Msa0977970	AT1G16710.7	43.380	793	321	15	50	719	879	1666	629

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Find 106 sgRNAs with CRISPR-Local

Find 945 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGAATTTGTTGCAC+AGG	0.445796	6_4:+12884937	Msa0977970:CDS
AAACGAGAAGATGCTTGCCA+AGG	0.601647	6_4:+12884305	Msa0977970:intron
AAAGAAATGATCATACACGT+TGG	0.679280	6_4:-12886417	None:intergenic
AAAGAAGAATTTGTTGCACA+GGG	0.561260	6_4:+12884938	Msa0977970:CDS
AACGAGAAGATGCTTGCCAA+GGG	0.580333	6_4:+12884306	Msa0977970:intron
AAGGCTCAGCATTACTTGCG+CGG	0.667558	6_4:-12897204	None:intergenic
AAGTGTGTTCTTCAGAACTA+TGG	0.361251	6_4:-12896655	None:intergenic
AATATCGCAAGTGAGCTACG+AGG	0.789451	6_4:-12896458	None:intergenic
AATGAAGAAAATATTGTCGT+TGG	0.489418	6_4:+12886389	Msa0977970:CDS
AATGATATCGTCCTTGAGAG+TGG	0.592327	6_4:+12896833	Msa0977970:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTGTGGAATTTCAACAATT+CGG	-	chr6_4:12883751-12883770	None:intergenic	30.0%
TTTGTACTAAATAGCGTATG+TGG	+	chr6_4:12883792-12883811	Msa0977970:intron	30.0%
GCTCATTAAAGCTAAAAGTT+GGG	-	chr6_4:12883918-12883937	None:intergenic	30.0%
AGCTCATTAAAGCTAAAAGT+TGG	-	chr6_4:12883919-12883938	None:intergenic	30.0%
TGGGATTGTTAACAGTAAAA+TGG	+	chr6_4:12884015-12884034	Msa0977970:intron	30.0%
GTTAACAGTAAAATGGTCTA+TGG	+	chr6_4:12884022-12884041	Msa0977970:intron	30.0%
TGAATTAAGCAATGAAGTAG+CGG	+	chr6_4:12884380-12884399	Msa0977970:CDS	30.0%
ACATATTAGTAGTCTGAGAA+AGG	+	chr6_4:12884521-12884540	Msa0977970:CDS	30.0%
TTTAACACAGATTACTTCTG+AGG	+	chr6_4:12884793-12884812	Msa0977970:intron	30.0%
CACAACATACACAAAAAAGT+GGG	-	chr6_4:12884886-12884905	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	12883272	12897682	12883272	ID=Msa0977970;Name=Msa0977970
chr6_4	mRNA	12883272	12897682	12883272	ID=Msa0977970-mRNA-1;Parent=Msa0977970;Name=Msa0977970-mRNA-1;_AED=0.15;_eAED=0.16;_QI=0\|0.23\|0.28\|0.92\|0.76\|0.78\|14\|37\|724
chr6_4	exon	12883272	12883277	12883272	ID=Msa0977970-mRNA-1:exon:4475;Parent=Msa0977970-mRNA-1
chr6_4	exon	12884315	12884554	12884315	ID=Msa0977970-mRNA-1:exon:4476;Parent=Msa0977970-mRNA-1
chr6_4	exon	12884803	12884959	12884803	ID=Msa0977970-mRNA-1:exon:4477;Parent=Msa0977970-mRNA-1
chr6_4	exon	12884996	12885078	12884996	ID=Msa0977970-mRNA-1:exon:4478;Parent=Msa0977970-mRNA-1
chr6_4	exon	12885213	12885434	12885213	ID=Msa0977970-mRNA-1:exon:4479;Parent=Msa0977970-mRNA-1
chr6_4	exon	12885571	12885699	12885571	ID=Msa0977970-mRNA-1:exon:4480;Parent=Msa0977970-mRNA-1
chr6_4	exon	12885779	12885979	12885779	ID=Msa0977970-mRNA-1:exon:4481;Parent=Msa0977970-mRNA-1
chr6_4	exon	12886087	12886229	12886087	ID=Msa0977970-mRNA-1:exon:4482;Parent=Msa0977970-mRNA-1
chr6_4	exon	12886364	12886610	12886364	ID=Msa0977970-mRNA-1:exon:4483;Parent=Msa0977970-mRNA-1
chr6_4	exon	12896423	12896562	12896423	ID=Msa0977970-mRNA-1:exon:4484;Parent=Msa0977970-mRNA-1
chr6_4	exon	12896652	12896709	12896652	ID=Msa0977970-mRNA-1:exon:4485;Parent=Msa0977970-mRNA-1
chr6_4	exon	12896797	12897024	12896797	ID=Msa0977970-mRNA-1:exon:4486;Parent=Msa0977970-mRNA-1
chr6_4	exon	12897157	12897389	12897157	ID=Msa0977970-mRNA-1:exon:4487;Parent=Msa0977970-mRNA-1
chr6_4	exon	12897558	12897682	12897558	ID=Msa0977970-mRNA-1:exon:4488;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12883272	12883277	12883272	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12884315	12884554	12884315	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12884803	12884959	12884803	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12884996	12885078	12884996	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12885213	12885434	12885213	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12885571	12885699	12885571	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12885779	12885979	12885779	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12886087	12886229	12886087	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12886364	12886610	12886364	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12896423	12896562	12896423	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12896652	12896709	12896652	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12896797	12897024	12896797	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12897157	12897389	12897157	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	CDS	12897558	12897645	12897558	ID=Msa0977970-mRNA-1:cds;Parent=Msa0977970-mRNA-1
chr6_4	three_prime_UTR	12897646	12897682	12897646	ID=Msa0977970-mRNA-1:three_prime_utr;Parent=Msa0977970-mRNA-1

Gene Sequence

>Msa0977970

ATGAATATGCTTGCCAAGGGTGGGAATACTTCATCAGTTGTCTCAAACATCTCGAGAAAAAGATGTTTTGATGAATTAAGCAATGAAGTAGCGGACTGGAGGTATCTTCCTGAAATGGTACGCAAACGTTATGATCAATCGATACACAAGACAATTCCCCCAAAAGTTTCCGTTTCCTTGATTGATTCGTTCAGACGTCACCAAATAGAAGAACATATTAGTAGTCTGAGAAAGGAATCTGATCAGATTACTTCTGAGGATGCAGCGGGAATTGATGTAAACACTTGCAACCTATGTAAAAGGGAAAGACTTTACTTTGCTAAAGTCCCACTTTTTTGTGTATGTTGTGGTGTCCGCATCAAGAAAATATATTTTTGTACAAAAGAAGAATTTGTTGCACAGGGTGAAAATGTCGCATTCAATGGGACATCTATTCCCAAGAAAAATCTTGCAAAAAGGAATAATGATGAAGTAATTGAAGAACCGCATCAAATATGTGCACTCTATAACAAGAAAGCAGATTTGGACTGCAGTGCTGAGTATATATGTCCTTTATGCCGCTTAAAAGAAATTGAAAATAGAATGCATGTTCCAAAAGCTGCTAACTTTGGTGCTAAGGACTTGACAAGAACTGTGCTAAGTGACCACTTAGAGAAAAGACTTTTCAAGCGTCTCATGCAAGAGAGAAAAAACTGGGGAAAAGATGAGGTTTTAGCAGTAGAAAGTCTTTCAATTAGAGAAGTGCTATCTGTCGACAAGCAATTGAAAGTGAATAAGCAGTTTCTGGACATCATCCCAGAGGAGAATTACCCTACTGAATTCTGTTATAGATCAAGGGTTATACTTCTGTTTCAGAAAATTGAAGGAGTAGATGTATGCATTTTTGGAATGTATGTCCAGGAGTTTGGCTCGGAATGTGGCACTCCGAATCAGCGATGTGTATACATTTCATATCTTGATTCCGTCAAGTATTTCAGGCCTAAAAGAAGGACAAAGAGTGGAGAAGCCCTTCGCACCTTCGTTTACCATGAGATATTGATTGGATATCTTTATTTTTGTAAGAAAAGGGGTTTCTCAACTTGCTACATATGGGCCTGTCCTCCAAAAAAAGGTGATGATTATATACTATATTGCCATCCCAAAGAGCAAAAGATTCCGAAGAAAGATAAGCTACGCCACTGGTATCTTTCAATGCTAAAAAAGGCTAATGAAGAAAATATTGTCGTTGGTTTAACCAACGTGTATGATCATTTCTTTGTTCCTACTGAAAAAAGGAAATCCAAGGTGACAGTTTCTCGTTTGCCATACTTTGATGGAGACTGGTGGTGCGGTAATGCTATGGTAGTTGCTGTAATACTCCGCATGTTAATTCAGTCAGTTATATGTATCAGTTATTCTATCCTTGGAAATTTTAAGAGAAGGACAATGGTTGGCCAAAATATATTGCCTACAAAAGAAAACTTCCTCGTAGCTCACTTGCGATATTCATGCATGCACTGCCGTGAAGTGATAGTTTCTGGGAAGCGTTGGTTTTGCACTGAATGCAAGAAATTTCAGGAGTGTGAAAGATGCCATAGTTCTGAAGAACACACTTTAATGAACGGCGAGGTGCATGCACTTTGTCAGGCTGCTGTTGATGATATCCCCTCCGAAACGAAACATAATGATATCGTCCTTGAGAGTGGATTGTTTGAAAATAGGGACAACTTTTTGATCTTTTGCCAGAAGTCTCAGTTTCAGTTTGATACACTCCGCCGTGCCAAGTATTCGTCTATGATGATCCTCTACCATCTACACAATCCTGCTGTGATGAGTCAAAATGAACACCAGTTAACTCAAAAGTGCTCAACACCAATGGTGAAACTGATGAACGTTTTGAAGCATGCATCTCAATGTCGTCGCCGCGCAAGTAATGCTGAGCCTTGCTCTCATCCAAACTGTTCTCAAATCAAGAAGTTGTTCTATCATGCCAGTAAGTGTGAAATTCGAGCTAATCGAGGCTGCCAGCATTGTAAAAAGATATGGTTTATACTAACTGTTCACTCCAGAAATTGCAAAGACTCGGAATGTAGAATACCACGCTGTAGTGATTTAAAGAAGCATTTAGAAAAGAGATCAAAGCAGTCTGAATCTTGCCGGAGAGCTGAAGTTTTGAAAGCAGTCATTGGCCAATGA

Protein sequence

>Msa0977970

MNMLAKGGNTSSVVSNISRKRCFDELSNEVADWRYLPEMVRKRYDQSIHKTIPPKVSVSLIDSFRRHQIEEHISSLRKESDQITSEDAAGIDVNTCNLCKRERLYFAKVPLFCVCCGVRIKKIYFCTKEEFVAQGENVAFNGTSIPKKNLAKRNNDEVIEEPHQICALYNKKADLDCSAEYICPLCRLKEIENRMHVPKAANFGAKDLTRTVLSDHLEKRLFKRLMQERKNWGKDEVLAVESLSIREVLSVDKQLKVNKQFLDIIPEENYPTEFCYRSRVILLFQKIEGVDVCIFGMYVQEFGSECGTPNQRCVYISYLDSVKYFRPKRRTKSGEALRTFVYHEILIGYLYFCKKRGFSTCYIWACPPKKGDDYILYCHPKEQKIPKKDKLRHWYLSMLKKANEENIVVGLTNVYDHFFVPTEKRKSKVTVSRLPYFDGDWWCGNAMVVAVILRMLIQSVICISYSILGNFKRRTMVGQNILPTKENFLVAHLRYSCMHCREVIVSGKRWFCTECKKFQECERCHSSEEHTLMNGEVHALCQAAVDDIPSETKHNDIVLESGLFENRDNFLIFCQKSQFQFDTLRRAKYSSMMILYHLHNPAVMSQNEHQLTQKCSTPMVKLMNVLKHASQCRRRASNAEPCSHPNCSQIKKLFYHASKCEIRANRGCQHCKKIWFILTVHSRNCKDSECRIPRCSDLKKHLEKRSKQSESCRRAEVLKAVIGQ*