AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0983910 MtrunA17_Chr6g0470151 82.044 362 55 1 1 362 1 352 2.33e-159 459
Msa0983910 MtrunA17_Chr2g0289181 47.210 233 123 0 327 559 1 233 4.95e-71 227
Msa0983910 MtrunA17_Chr6g0470161 80.000 125 20 1 435 559 1 120 1.99e-61 198
Msa0983910 MtrunA17_Chr7g0266611 49.697 165 82 1 395 559 2 165 1.18e-34 128
Msa0983910 MtrunA17_Chr8g0366921 49.462 186 77 5 1 180 1 175 7.65e-28 114
Msa0983910 MtrunA17_Chr1g0202461 40.800 125 63 2 291 415 222 335 2.02e-26 111
Msa0983910 MtrunA17_Chr8g0368721 40.950 337 158 7 1 321 1 312 6.08e-25 105
Msa0983910 MtrunA17_Chr8g0348311 46.939 98 49 2 317 414 134 228 4.26e-23 100
Msa0983910 MtrunA17_Chr7g0248691 43.243 111 54 2 312 414 118 227 2.78e-22 98.2
Msa0983910 MtrunA17_Chr7g0270051 45.977 87 47 0 333 419 46 132 8.81e-20 89.4
Msa0983910 MtrunA17_Chr8g0387501 43.810 105 49 3 341 439 130 230 3.37e-19 87.8
Msa0983910 MtrunA17_Chr7g0266621 36.795 337 162 7 1 321 1 302 3.63e-19 88.6
Msa0983910 MtrunA17_Chr2g0293481 49.351 77 38 1 341 417 268 343 1.03e-18 89.4
Msa0983910 MtrunA17_Chr7g0238921 42.453 106 49 3 292 397 87 180 1.97e-17 83.6
Msa0983910 MtrunA17_Chr2g0319961 52.381 63 30 0 341 403 81 143 8.55e-16 77.8
Msa0983910 MtrunA17_Chr5g0401821 43.529 85 45 1 316 397 172 256 8.64e-16 77.8
Msa0983910 MtrunA17_Chr8g0379461 49.206 63 32 0 341 403 77 139 1.08e-15 77.0
Msa0983910 MtrunA17_Chr2g0293901 46.154 182 82 5 1 181 1 167 1.31e-15 75.9
Msa0983910 MtrunA17_Chr2g0284321 51.667 60 29 0 341 400 36 95 1.48e-15 75.9
Msa0983910 MtrunA17_Chr1g0184691 43.902 82 43 1 321 399 271 352 4.29e-15 77.0
Msa0983910 MtrunA17_Chr4g0071521 42.353 85 46 1 316 397 218 302 4.58e-15 76.3
Msa0983910 MtrunA17_Chr8g0387981 39.130 92 50 2 310 395 98 189 8.71e-15 76.3
Msa0983910 MtrunA17_Chr4g0053281 50.000 62 31 0 341 402 267 328 1.17e-14 75.5
Msa0983910 MtrunA17_Chr7g0228941 44.118 68 38 0 334 401 46 113 1.98e-14 70.1
Msa0983910 MtrunA17_Chr3g0139021 38.889 90 54 1 309 397 282 371 2.38e-14 75.1
Msa0983910 MtrunA17_Chr4g0042831 38.710 93 52 2 338 426 276 367 5.35e-14 73.9
Msa0983910 MtrunA17_Chr7g0218081 42.424 66 37 1 338 403 408 472 9.12e-14 73.9
Msa0983910 MtrunA17_Chr1g0147881 33.684 95 58 1 320 409 237 331 1.46e-13 72.0
Msa0983910 MtrunA17_Chr5g0418191 42.029 69 39 1 338 406 318 385 3.50e-13 71.6
Msa0983910 MtrunA17_Chr2g0302391 45.000 60 33 0 341 400 212 271 9.99e-13 70.5
Msa0983910 MtrunA17_Chr4g0074001 26.633 199 133 7 291 483 313 504 1.46e-12 70.1
Msa0983910 MtrunA17_Chr4g0051881 47.541 61 31 1 340 400 178 237 1.60e-12 68.9
Msa0983910 MtrunA17_Chr7g0263531 48.333 60 30 1 341 400 362 420 1.73e-12 69.7
Msa0983910 MtrunA17_Chr5g0443131 48.387 62 31 1 340 401 157 217 2.36e-12 68.2
Msa0983910 MtrunA17_Chr7g0249311 44.928 69 36 2 332 400 171 237 5.43e-12 68.6
Msa0983910 MtrunA17_Chr7g0249311 40.909 66 38 1 340 405 392 456 1.26e-11 67.4
Msa0983910 MtrunA17_Chr2g0288871 33.981 103 64 3 299 400 266 365 8.02e-12 67.8
Msa0983910 MtrunA17_Chr3g0129321 37.681 69 40 3 337 404 115 181 9.68e-12 64.7
Msa0983910 MtrunA17_Chr1g0194481 41.429 70 40 1 340 409 158 226 1.12e-11 66.2
Msa0983910 MtrunA17_Chr3g0079691 48.387 62 30 2 339 400 279 338 1.22e-11 67.4
Msa0983910 MtrunA17_Chr3g0079691 37.500 64 39 1 340 403 495 557 4.37e-11 65.9
Msa0983910 MtrunA17_Chr4g0001361 40.964 83 48 1 335 416 157 239 1.51e-11 65.9
Msa0983910 MtrunA17_Chr3g0136961 39.286 84 50 1 315 397 112 195 1.67e-11 65.5
Msa0983910 MtrunA17_Chr3g0099641 37.349 83 49 2 318 400 232 311 1.72e-11 66.6
Msa0983910 MtrunA17_Chr7g0243891 47.059 68 34 2 333 400 209 274 1.98e-11 66.6
Msa0983910 MtrunA17_Chr7g0249301 40.625 64 37 1 340 403 292 354 2.94e-11 65.5
Msa0983910 MtrunA17_Chr5g0445311 37.313 67 41 1 340 406 364 429 3.84e-11 65.9
Msa0983910 MtrunA17_Chr3g0101131 37.313 67 41 1 340 406 415 480 4.42e-11 65.5
Msa0983910 MtrunA17_Chr1g0150281 41.667 72 42 0 341 412 141 212 4.55e-11 63.9
Msa0983910 MtrunA17_Chr8g0347251 41.573 89 46 2 313 395 105 193 6.05e-11 64.3
Msa0983910 MtrunA17_Chr1g0150271 33.884 121 72 2 306 421 65 182 6.67e-11 63.2
Msa0983910 MtrunA17_Chr5g0447701 34.513 113 60 3 304 403 203 314 7.72e-11 64.3
Msa0983910 MtrunA17_Chr2g0294231 43.548 62 34 1 340 401 203 263 8.63e-11 63.9
Msa0983910 MtrunA17_Chr7g0270831 39.683 63 38 0 338 400 214 276 9.89e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0983910 AT5G52830.1 51.020 98 46 2 318 415 144 239 9.26e-25 106
Msa0983910 AT4G01250.1 41.176 119 62 3 317 430 107 222 2.03e-22 98.2
Msa0983910 AT4G23550.1 46.512 86 45 1 332 417 132 216 1.44e-19 90.1
Msa0983910 AT1G30650.1 39.655 116 65 2 294 409 175 285 9.77e-18 86.3
Msa0983910 AT5G45050.4 31.579 152 102 2 258 409 960 1109 1.01e-17 87.8
Msa0983910 AT5G45050.1 31.579 152 102 2 258 409 1099 1248 1.19e-17 87.4
Msa0983910 AT5G45050.2 31.579 152 102 2 258 409 1071 1220 1.38e-17 87.4
Msa0983910 AT2G34830.1 52.174 69 32 1 341 409 216 283 1.79e-17 85.5
Msa0983910 AT2G34830.2 52.174 69 32 1 341 409 225 292 1.94e-17 85.5
Msa0983910 AT5G28650.1 40.217 92 52 1 311 399 230 321 6.32e-17 82.8
Msa0983910 AT3G04670.1 35.593 118 62 3 285 399 215 321 6.74e-17 82.4
Msa0983910 AT2G23320.1 42.353 85 46 1 316 397 213 297 3.12e-16 80.5
Msa0983910 AT4G24240.1 39.785 93 53 1 308 397 246 338 4.73e-15 77.4
Msa0983910 AT3G58710.1 49.231 65 33 0 341 405 72 136 7.29e-15 75.5
Msa0983910 AT3G58710.2 48.485 66 34 0 341 406 71 136 7.33e-15 75.5
Msa0983910 AT1G29280.1 50.000 60 30 0 341 400 75 134 1.91e-14 73.9
Msa0983910 AT4G31550.2 37.000 100 60 1 309 405 211 310 2.15e-14 75.1
Msa0983910 AT4G31550.1 37.634 93 55 1 316 405 219 311 3.31e-14 74.3
Msa0983910 AT2G24570.1 37.931 87 51 1 316 399 216 302 7.46e-14 73.2
Msa0983910 AT2G03340.1 42.029 69 39 1 338 406 413 480 3.14e-13 72.8
Msa0983910 AT2G30590.1 37.931 87 51 1 316 399 286 372 4.95e-13 71.2
Msa0983910 AT5G45260.1 30.128 156 91 6 254 403 1130 1273 6.41e-13 72.4
Msa0983910 AT2G04880.1 46.032 63 33 1 341 403 308 369 9.80e-13 70.9
Msa0983910 AT2G04880.2 46.032 63 33 1 341 403 284 345 9.98e-13 70.9
Msa0983910 AT1G80840.1 32.836 134 79 3 340 471 146 270 1.01e-12 69.7
Msa0983910 AT5G43290.1 39.726 73 43 1 325 397 99 170 1.70e-12 68.6
Msa0983910 AT3G01080.2 41.270 63 36 1 338 400 342 403 2.91e-12 69.3
Msa0983910 AT3G01080.1 41.270 63 36 1 338 400 304 365 2.97e-12 69.3
Msa0983910 AT2G47260.1 39.773 88 51 2 313 400 148 233 4.39e-12 68.2
Msa0983910 AT4G31800.2 44.000 75 39 1 340 411 111 185 4.94e-12 66.6
Msa0983910 AT5G07100.1 45.455 66 35 1 335 400 112 176 8.20e-12 67.0
Msa0983910 AT4G18170.1 36.538 104 60 2 302 400 129 231 8.48e-12 67.0
Msa0983910 AT5G07100.2 45.455 66 35 1 335 400 19 83 1.04e-11 65.1
Msa0983910 AT5G07100.4 45.455 66 35 1 335 400 19 83 1.04e-11 65.1
Msa0983910 AT5G07100.5 45.455 66 35 1 335 400 19 83 1.04e-11 65.1
Msa0983910 AT5G07100.3 45.455 66 35 1 335 400 19 83 1.04e-11 65.1
Msa0983910 AT2G37260.1 38.272 81 45 2 321 397 326 405 1.07e-11 67.4
Msa0983910 AT1G29860.1 45.161 62 33 1 340 401 136 196 1.10e-11 66.2
Msa0983910 AT2G37260.2 38.272 81 45 2 321 397 244 323 1.16e-11 67.0
Msa0983910 AT2G37260.3 38.272 81 45 2 321 397 246 325 1.17e-11 67.0
Msa0983910 AT4G31800.1 44.000 75 39 1 340 411 176 250 1.19e-11 66.6
Msa0983910 AT2G25000.2 47.541 61 32 0 340 400 103 163 1.30e-11 65.1
Msa0983910 AT2G25000.4 47.541 61 32 0 340 400 103 163 1.30e-11 65.1
Msa0983910 AT2G25000.3 47.541 61 32 0 340 400 125 185 1.40e-11 65.5
Msa0983910 AT2G46400.1 39.785 93 49 2 310 395 69 161 1.72e-11 65.9
Msa0983910 AT1G13960.1 41.270 63 36 1 338 400 407 468 1.79e-11 67.0
Msa0983910 AT1G13960.2 41.270 63 36 1 338 400 254 315 1.87e-11 66.2
Msa0983910 AT4G26640.7 39.744 78 44 3 338 414 169 244 3.38e-11 66.2
Msa0983910 AT4G26640.2 39.744 78 44 3 338 414 209 284 3.56e-11 66.2
Msa0983910 AT2G25000.1 47.541 61 32 0 340 400 146 206 3.61e-11 64.3
Msa0983910 AT4G26640.1 39.744 78 44 3 338 414 137 212 3.87e-11 65.9
Msa0983910 AT1G80590.1 45.570 79 40 2 341 417 86 163 3.90e-11 63.9
Msa0983910 AT4G30935.1 39.683 63 37 1 341 403 332 393 4.03e-11 65.9
Msa0983910 AT4G11070.2 38.235 102 57 2 300 395 65 166 4.21e-11 64.3
Msa0983910 AT4G26640.5 39.744 78 44 3 338 414 33 108 4.53e-11 65.5
Msa0983910 AT4G26640.6 39.744 78 44 3 338 414 33 108 4.53e-11 65.5
Msa0983910 AT4G26640.4 39.744 78 44 3 338 414 33 108 4.53e-11 65.5
Msa0983910 AT4G26640.3 39.744 78 44 3 338 414 33 108 4.53e-11 65.5
Msa0983910 AT2G40750.1 47.541 61 30 1 337 395 149 209 5.65e-11 64.7
Msa0983910 AT4G11070.1 38.235 102 57 2 300 395 97 198 5.81e-11 64.3
Msa0983910 AT4G23810.1 41.176 85 44 2 317 395 131 215 5.88e-11 64.3
Msa0983910 AT1G69310.4 44.068 59 32 1 340 398 147 204 7.15e-11 63.9
Msa0983910 AT1G69310.2 44.068 59 32 1 340 398 147 204 7.15e-11 63.9
Msa0983910 AT1G69310.1 44.068 59 32 1 340 398 147 204 7.15e-11 63.9
Msa0983910 AT5G46350.1 39.706 68 40 1 340 407 183 249 8.19e-11 63.9
Msa0983910 AT2G21900.1 43.548 62 34 1 340 401 109 169 9.64e-11 62.0

Find 144 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCAGGAAACAAACTTAT+TGG 0.153110 6_4:+27275257 Msa0983910:CDS
TTTCTCTAGCAGCAGGTAAA+TGG 0.191301 6_4:-27275121 None:intergenic
TACCCGCTGTTGGAAATTTC+AGG 0.250971 6_4:+27274964 Msa0983910:CDS
TTGAGGAGATATGACGTTAT+TGG 0.276825 6_4:-27272798 None:intergenic
GGTGGGAGTATTGAAATTAT+TGG 0.287618 6_4:-27273224 None:intergenic
TGGTGGTCGTGGACAAAATA+TGG 0.290490 6_4:+27273792 Msa0983910:CDS
TAGTCTTTGATGACTTGTTC+TGG 0.291346 6_4:-27274938 None:intergenic
TGTGATTGATTGAGCTTTCT+TGG 0.296315 6_4:-27273526 None:intergenic
TATGTTAGAGGATTTCTTAT+TGG 0.311118 6_4:+27275325 Msa0983910:CDS
TTGGGATAGTGGTGGTAGTT+GGG 0.331508 6_4:-27272967 None:intergenic
AACATTATCACTAGTGTTCA+TGG 0.339452 6_4:+27273379 Msa0983910:CDS
GTGATTGATTGAGCTTTCTT+GGG 0.341470 6_4:-27273525 None:intergenic
CTAAAACCATCAAAGTAAAA+TGG 0.342350 6_4:-27272830 None:intergenic
TTGGTAGGGGTGGGGTTAAC+AGG 0.344695 6_4:-27273205 None:intergenic
TAGTGGTGGTTGAAGGGGTT+TGG 0.351715 6_4:-27273030 None:intergenic
AGTGGTGGTAGTTGGGATGG+GGG 0.353665 6_4:-27272960 None:intergenic
TAGTGGTGGTAGTTGGGATG+GGG 0.353957 6_4:-27272961 None:intergenic
GAACCCATCTTTGAAATGTT+TGG 0.367325 6_4:-27274993 None:intergenic
CAAATAAGCTTTCAGAAGAT+TGG 0.368905 6_4:+27273772 Msa0983910:CDS
AGTCTTTGATGACTTGTTCT+GGG 0.370924 6_4:-27274937 None:intergenic
GAGGTGGTGGTGACAGTGAT+TGG 0.371002 6_4:-27272770 None:intergenic
TGTAATGAGGGTTGGGATAG+TGG 0.371786 6_4:-27272978 None:intergenic
GGGTTTGGATAATAGTGATT+GGG 0.385706 6_4:-27273015 None:intergenic
GTTGGGATAGTGGTGGTAGT+TGG 0.390091 6_4:-27272968 None:intergenic
CCGGGAATAGTGGTGGTTGA+AGG 0.395917 6_4:-27273037 None:intergenic
CCTTCAACCACCACTATTCC+CGG 0.405389 6_4:+27273037 Msa0983910:CDS
AATTAACAACAAGGTAACTT+TGG 0.407679 6_4:+27273734 Msa0983910:CDS
GTCAAGTTGGTGGTGGTGCT+AGG 0.408314 6_4:-27273355 None:intergenic
ATGGAGAGAGGTGGTGGGAT+TGG 0.408785 6_4:-27272936 None:intergenic
AATTACCCAACACCAGATCT+AGG 0.412137 6_4:+27273554 Msa0983910:CDS
ATAGTGGTGGTAGTTGGGAT+GGG 0.414168 6_4:-27272962 None:intergenic
TGAAATTATTGGTAGGGGTG+GGG 0.414985 6_4:-27273213 None:intergenic
GTGATTCACCGGAGTAAACC+TGG 0.417652 6_4:-27275226 None:intergenic
CAAAACATAATTCAAGAATC+AGG 0.420705 6_4:+27273605 Msa0983910:CDS
CAACAAGAAATGCAATCAAA+TGG 0.425635 6_4:+27273148 Msa0983910:CDS
AAGCATGACTGATATGTTAG+AGG 0.427454 6_4:+27275313 Msa0983910:CDS
GACGTTATTGGTGGTGGAGG+TGG 0.428662 6_4:-27272786 None:intergenic
GTGGTGGTGCTAGGGCTAAT+AGG 0.429133 6_4:-27273346 None:intergenic
GTCACCAAACATTTCAAAGA+TGG 0.429508 6_4:+27274989 Msa0983910:CDS
GAATCACAAATCCTTCCTTC+AGG 0.431729 6_4:+27275242 Msa0983910:CDS
TTGGTGTTTGAGATGGAGAT+GGG 0.438498 6_4:-27272751 None:intergenic
TCCAATGGACATTGACGATT+GGG 0.439428 6_4:+27272661 None:intergenic
CACAACAAAAGTGTCCTCTT+TGG 0.441667 6_4:-27274850 None:intergenic
AAGGCGTTGGTAGTGGTCAT+GGG 0.441960 6_4:-27273280 None:intergenic
ATTCCCGGCCACATAACATT+TGG 0.443207 6_4:+27273052 Msa0983910:CDS
TTGAAATTATTGGTAGGGGT+GGG 0.444762 6_4:-27273214 None:intergenic
AACACTTGACTGTTATTGTT+TGG 0.445737 6_4:-27273463 None:intergenic
TGAAATGGTAGGATATTGAT+TGG 0.447083 6_4:-27273404 None:intergenic
AAGGAACAGGGTTATCGTCT+TGG 0.451273 6_4:-27272859 None:intergenic
TTCTCTAGCAGCAGGTAAAT+GGG 0.451842 6_4:-27275120 None:intergenic
ACTTGAATATTCCTGCTCTC+TGG 0.454557 6_4:-27275047 None:intergenic
GATAGTGGTGGTAGTTGGGA+TGG 0.455456 6_4:-27272963 None:intergenic
ATAAGTTCAGTTTCTGAATG+AGG 0.455899 6_4:-27275092 None:intergenic
GTTATTGGTGGTGGAGGTGG+TGG 0.460419 6_4:-27272783 None:intergenic
AGTGATAATGTTAGTCAAGT+TGG 0.462717 6_4:-27273368 None:intergenic
TAAGGCGTTGGTAGTGGTCA+TGG 0.465082 6_4:-27273281 None:intergenic
AGGAACAGGGTTATCGTCTT+GGG 0.469719 6_4:-27272858 None:intergenic
GCAGGTAAATGGAACTCTAT+TGG 0.471063 6_4:+27274906 Msa0983910:CDS
GGGGTTTGGATAATAGTGAT+TGG 0.471326 6_4:-27273016 None:intergenic
ATTAGGTAAAATCCTAGATC+TGG 0.473232 6_4:-27273566 None:intergenic
ATGAAGTGGTTGTTGTAATG+AGG 0.474053 6_4:-27272991 None:intergenic
GGAGTATTGAAATTATTGGT+AGG 0.476280 6_4:-27273220 None:intergenic
CTCCAATGGACATTGACGAT+TGG 0.476413 6_4:+27272660 None:intergenic
ACCTGGGCATTGCTACTCAT+TGG 0.480044 6_4:-27275209 None:intergenic
TGGTTGTTGTAATGAGGGTT+GGG 0.482879 6_4:-27272985 None:intergenic
ATAGAGTTCCATTTACCTGC+TGG 0.487185 6_4:-27274903 None:intergenic
GAGTATTGAAATTATTGGTA+GGG 0.491396 6_4:-27273219 None:intergenic
GATTCAATTTCTCTAGCAGC+AGG 0.497334 6_4:-27275128 None:intergenic
AGATATGACGTTATTGGTGG+TGG 0.499087 6_4:-27272792 None:intergenic
AACAAGAAATGCAATCAAAT+GGG 0.503558 6_4:+27273149 Msa0983910:CDS
TCAAGTTGGTGGTGGTGCTA+GGG 0.503913 6_4:-27273354 None:intergenic
TGTTATGTGGCCGGGAATAG+TGG 0.508241 6_4:-27273047 None:intergenic
TAAGTTCAGTTTCTGAATGA+GGG 0.508550 6_4:-27275091 None:intergenic
GTTAGCTAAGGCGTTGGTAG+TGG 0.511992 6_4:-27273287 None:intergenic
AGCTTTCTTGGGGATTGTTG+TGG 0.514180 6_4:-27273514 None:intergenic
CGGGAATAGTGGTGGTTGAA+GGG 0.514280 6_4:-27273036 None:intergenic
TCTTGTTGTTCGATAAGTGT+TGG 0.517204 6_4:-27273133 None:intergenic
TAAGTTTGTTTCCTGAAGGA+AGG 0.517251 6_4:-27275253 None:intergenic
GGTGGAAGGAGTAGTGAGGG+TGG 0.517876 6_4:-27273242 None:intergenic
GTGGTTGTTGTAATGAGGGT+TGG 0.523332 6_4:-27272986 None:intergenic
AGTATTGAAATTATTGGTAG+GGG 0.523845 6_4:-27273218 None:intergenic
AAAATCCTAGATCTGGTGTT+GGG 0.524682 6_4:-27273559 None:intergenic
CTAATAGGTGCAGTGGTGGT+CGG 0.525114 6_4:-27273331 None:intergenic
AAGACTAGATTACCCGCTGT+TGG 0.528458 6_4:+27274954 Msa0983910:CDS
TCACCAAACATTTCAAAGAT+GGG 0.532829 6_4:+27274990 Msa0983910:CDS
ATTGAAATTATTGGTAGGGG+TGG 0.533722 6_4:-27273215 None:intergenic
CCAATAAGTTTGTTTCCTGA+AGG 0.534237 6_4:-27275257 None:intergenic
GTACCAAATGTTATGTGGCC+GGG 0.534649 6_4:-27273055 None:intergenic
GAGTATTGCATACTCTGAAA+TGG 0.535792 6_4:-27273419 None:intergenic
AGGAGATATGACGTTATTGG+TGG 0.536868 6_4:-27272795 None:intergenic
TCCCAATCGTCAATGTCCAT+TGG 0.539210 6_4:-27272662 None:intergenic
AGGAAGGATTTGTGATTCAC+CGG 0.548075 6_4:-27275237 None:intergenic
GGTTTGGATAATAGTGATTG+GGG 0.549958 6_4:-27273014 None:intergenic
TGAAGTGGTTGTTGTAATGA+GGG 0.550704 6_4:-27272990 None:intergenic
AAACCATCAAAGTAAAATGG+TGG 0.550726 6_4:-27272827 None:intergenic
ATTGGTGTTTGAGATGGAGA+TGG 0.554952 6_4:-27272752 None:intergenic
ATCACTAACACTAGTGCTCA+TGG 0.555133 6_4:+27273079 Msa0983910:CDS
ATAAGCTTTCAGAAGATTGG+TGG 0.556521 6_4:+27273775 Msa0983910:CDS
AGCTTTGTTGTTATTGACAA+AGG 0.557392 6_4:-27272693 None:intergenic
GGGAATAGTGGTGGTTGAAG+GGG 0.558417 6_4:-27273035 None:intergenic
TAAAATCCTAGATCTGGTGT+TGG 0.562048 6_4:-27273560 None:intergenic
TATGACGTTATTGGTGGTGG+AGG 0.562705 6_4:-27272789 None:intergenic
ACAGTGATTGGTGTTTGAGA+TGG 0.562764 6_4:-27272758 None:intergenic
TCTAACATATCAGTCATGCT+TGG 0.562921 6_4:-27275311 None:intergenic
AGTTGTTGTGGGGTTAGCTA+AGG 0.564800 6_4:-27273299 None:intergenic
TGATTGATTGAGCTTTCTTG+GGG 0.565498 6_4:-27273524 None:intergenic
GTAATTCTAGTATGGGTGGA+AGG 0.566336 6_4:-27273256 None:intergenic
GCCAATGAGTAGCAATGCCC+AGG 0.566515 6_4:+27275208 Msa0983910:CDS
AAAAGAGGCGGGGTTAGTAA+AGG 0.569673 6_4:-27272720 None:intergenic
GGGGCATGGAGAGAGGTGGT+GGG 0.569845 6_4:-27272941 None:intergenic
CAGGGTTATCGTCTTGGGCA+AGG 0.570064 6_4:-27272853 None:intergenic
ATGTCGATCATCCAGAGAGC+AGG 0.571282 6_4:+27275036 Msa0983910:CDS
TTTCAGAAGATTGGTGGTCG+TGG 0.572428 6_4:+27273781 Msa0983910:CDS
TGTACCAAATGTTATGTGGC+CGG 0.574015 6_4:-27273056 None:intergenic
AATAGTGATTGGGGATGAAG+TGG 0.575835 6_4:-27273005 None:intergenic
AGCAATGCCCAGGTTTACTC+CGG 0.575866 6_4:+27275218 Msa0983910:CDS
ATGTGAAAAGACCTTGGATG+TGG 0.577521 6_4:-27273837 None:intergenic
GTGGAAGGAGTAGTGAGGGT+GGG 0.580092 6_4:-27273241 None:intergenic
AGGCGTTGGTAGTGGTCATG+GGG 0.582833 6_4:-27273279 None:intergenic
AACCATCAAAGTAAAATGGT+GGG 0.584704 6_4:-27272826 None:intergenic
TATGGGTGGAAGGAGTAGTG+AGG 0.591730 6_4:-27273246 None:intergenic
CTTTGATGACTTGTTCTGGG+AGG 0.593215 6_4:-27274934 None:intergenic
TGGTGTTTGAGATGGAGATG+GGG 0.596571 6_4:-27272750 None:intergenic
TGATTGTACCAAATGTTATG+TGG 0.596955 6_4:-27273060 None:intergenic
AAAGCTTATTTGTTCTCACA+TGG 0.598586 6_4:-27273761 None:intergenic
GGTAAATGGAACTCTATTGG+TGG 0.600259 6_4:+27274909 Msa0983910:CDS
ATGGGTGGAAGGAGTAGTGA+GGG 0.602721 6_4:-27273245 None:intergenic
GACCTGAAATTTCCAACAGC+GGG 0.604025 6_4:-27274966 None:intergenic
ATTGCATACTCTGAAATGGT+AGG 0.607029 6_4:-27273415 None:intergenic
GCTAGGGCTAATAGGTGCAG+TGG 0.611408 6_4:-27273338 None:intergenic
TGATTCACCGGAGTAAACCT+GGG 0.612077 6_4:-27275225 None:intergenic
TGTGGGGTTAGCTAAGGCGT+TGG 0.612971 6_4:-27273293 None:intergenic
GAAATAGAAAACCATCATTG+AGG 0.620026 6_4:-27275358 None:intergenic
TTAGCGGGTCCCCACATCCA+AGG 0.623038 6_4:+27273826 Msa0983910:CDS
TGTGAAAAGACCTTGGATGT+GGG 0.630725 6_4:-27273836 None:intergenic
GATAATGTTAGTCAAGTTGG+TGG 0.636625 6_4:-27273365 None:intergenic
GTGAAAAGACCTTGGATGTG+GGG 0.637723 6_4:-27273835 None:intergenic
AGGGCTAATAGGTGCAGTGG+TGG 0.638903 6_4:-27273335 None:intergenic
AATGTTAGTCAAGTTGGTGG+TGG 0.642962 6_4:-27273362 None:intergenic
ATAGAAAACCATCATTGAGG+TGG 0.645904 6_4:-27275355 None:intergenic
GGCGTTGGTAGTGGTCATGG+GGG 0.697585 6_4:-27273278 None:intergenic
CGACCTGAAATTTCCAACAG+CGG 0.704425 6_4:-27274967 None:intergenic
AATGAGGGTTGGGATAGTGG+TGG 0.711606 6_4:-27272975 None:intergenic
TATGTGGCCGGGAATAGTGG+TGG 0.724076 6_4:-27273044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAATAAATAAATTTTAT+GGG - chr6_4:27274276-27274295 None:intergenic 0.0%
!!! TTTAAAATAAATAAATTTTA+TGG - chr6_4:27274277-27274296 None:intergenic 0.0%
!!! TATCAAAATTAATGTATTTT+TGG + chr6_4:27274133-27274152 Msa0983910:intron 10.0%
!!! TAAATATTTTACATATAACA+AGG + chr6_4:27274346-27274365 Msa0983910:intron 10.0%
!! ACTAAAATTATATGTTTATT+GGG - chr6_4:27274690-27274709 None:intergenic 10.0%
!! TACTAAAATTATATGTTTAT+TGG - chr6_4:27274691-27274710 None:intergenic 10.0%
!!! ATATGTAAAAGTTAGATTTT+TGG - chr6_4:27273863-27273882 None:intergenic 15.0%
!!! CTAATGTTTAAATATGTTTT+TGG - chr6_4:27274489-27274508 None:intergenic 15.0%
!! AAATTAATCTTATCAAGTAT+TGG - chr6_4:27274547-27274566 None:intergenic 15.0%
!! AATACTTGATAAGATTAATT+TGG + chr6_4:27274546-27274565 Msa0983910:intron 15.0%
!!! TGAAATCATTTTGTTTAAGT+GGG - chr6_4:27272889-27272908 None:intergenic 20.0%
!!! ATGAAATCATTTTGTTTAAG+TGG - chr6_4:27272890-27272909 None:intergenic 20.0%
!! TTACAGAAAAATTAACAACA+AGG + chr6_4:27273725-27273744 Msa0983910:intron 20.0%
!!! TTTTTGGTTTAAGTACAAAA+TGG + chr6_4:27274149-27274168 Msa0983910:intron 20.0%
!!! TAACAATCAAAACTTTTACA+TGG - chr6_4:27274194-27274213 None:intergenic 20.0%
!!! ATTTTAGTCGATCATAAATA+AGG - chr6_4:27274326-27274345 None:intergenic 20.0%
!! AATTTATTCGTAGGATAATT+TGG - chr6_4:27274373-27274392 None:intergenic 20.0%
!! TTCACTTTGTATTTATGATA+AGG + chr6_4:27274512-27274531 Msa0983910:intron 20.0%
!! TCACTTTGTATTTATGATAA+GGG + chr6_4:27274513-27274532 Msa0983910:intron 20.0%
!! TGTAACATTTATGTAAAACT+TGG + chr6_4:27274664-27274683 Msa0983910:intron 20.0%
!! ATTAAAGTGATACAATTTAC+AGG + chr6_4:27274724-27274743 Msa0983910:intron 20.0%
!!! AAAACAAGTTTTCAACATTA+GGG - chr6_4:27275157-27275176 None:intergenic 20.0%
! CTAAAACCATCAAAGTAAAA+TGG - chr6_4:27272833-27272852 None:intergenic 25.0%
!!! GAAATCATTTTGTTTAAGTG+GGG - chr6_4:27272888-27272907 None:intergenic 25.0%
! AACAAGAAATGCAATCAAAT+GGG + chr6_4:27273149-27273168 Msa0983910:CDS 25.0%
!! AGTATTGAAATTATTGGTAG+GGG - chr6_4:27273221-27273240 None:intergenic 25.0%
!!! GAGTATTGAAATTATTGGTA+GGG - chr6_4:27273222-27273241 None:intergenic 25.0%
! CAAAACATAATTCAAGAATC+AGG + chr6_4:27273605-27273624 Msa0983910:CDS 25.0%
! AATTAACAACAAGGTAACTT+TGG + chr6_4:27273734-27273753 Msa0983910:CDS 25.0%
! TATGGACAAAAAAACATTAG+CGG + chr6_4:27273810-27273829 Msa0983910:CDS 25.0%
! CAACAAGTAGAAAAAACAAA+AGG - chr6_4:27274241-27274260 None:intergenic 25.0%
!!! TTTCTACTTGTTGTTTGTTT+TGG + chr6_4:27274248-27274267 Msa0983910:intron 25.0%
!! AAACAAGTTTTCAACATTAG+GGG - chr6_4:27275156-27275175 None:intergenic 25.0%
!! GAAAACAAGTTTTCAACATT+AGG - chr6_4:27275158-27275177 None:intergenic 25.0%
! TATGTTAGAGGATTTCTTAT+TGG + chr6_4:27275325-27275344 Msa0983910:CDS 25.0%
!! AGCTTTGTTGTTATTGACAA+AGG - chr6_4:27272696-27272715 None:intergenic 30.0%
!! TAAAATGGTGGGATATTTTG+AGG - chr6_4:27272818-27272837 None:intergenic 30.0%
AACCATCAAAGTAAAATGGT+GGG - chr6_4:27272829-27272848 None:intergenic 30.0%
AAACCATCAAAGTAAAATGG+TGG - chr6_4:27272830-27272849 None:intergenic 30.0%
!! TTTTGTTTAAGTGGGGAAAA+AGG - chr6_4:27272881-27272900 None:intergenic 30.0%
TGATTGTACCAAATGTTATG+TGG - chr6_4:27273063-27273082 None:intergenic 30.0%
!! AAGTGTTGGACAATCAAAAA+AGG - chr6_4:27273122-27273141 None:intergenic 30.0%
CAACAAGAAATGCAATCAAA+TGG + chr6_4:27273148-27273167 Msa0983910:CDS 30.0%
!! GGAGTATTGAAATTATTGGT+AGG - chr6_4:27273223-27273242 None:intergenic 30.0%
! AGTGATAATGTTAGTCAAGT+TGG - chr6_4:27273371-27273390 None:intergenic 30.0%
AACATTATCACTAGTGTTCA+TGG + chr6_4:27273379-27273398 Msa0983910:CDS 30.0%
TGAAATGGTAGGATATTGAT+TGG - chr6_4:27273407-27273426 None:intergenic 30.0%
AACACTTGACTGTTATTGTT+TGG - chr6_4:27273466-27273485 None:intergenic 30.0%
ATTAGGTAAAATCCTAGATC+TGG - chr6_4:27273569-27273588 None:intergenic 30.0%
! GCTTTTTGTGAATCTGAATT+AGG - chr6_4:27273586-27273605 None:intergenic 30.0%
AAAGCTTATTTGTTCTCACA+TGG - chr6_4:27273764-27273783 None:intergenic 30.0%
CAAATAAGCTTTCAGAAGAT+TGG + chr6_4:27273772-27273791 Msa0983910:CDS 30.0%
ATGGACAAAAAAACATTAGC+GGG + chr6_4:27273811-27273830 Msa0983910:CDS 30.0%
TCTAATACAACATGATACCT+AGG + chr6_4:27273921-27273940 Msa0983910:intron 30.0%
CTAATACAACATGATACCTA+GGG + chr6_4:27273922-27273941 Msa0983910:intron 30.0%
!!! ATACCTAGGGTTTTAAATAG+TGG + chr6_4:27273935-27273954 Msa0983910:intron 30.0%
TCTCTGCAAAATTTATTCGT+AGG - chr6_4:27274382-27274401 None:intergenic 30.0%
!!! CATCTTTGTTTTCTATCTGT+TGG - chr6_4:27274405-27274424 None:intergenic 30.0%
ACAAATATATCACTCGTTTC+AGG + chr6_4:27274626-27274645 Msa0983910:intron 30.0%
CAAATATATCACTCGTTTCA+GGG + chr6_4:27274627-27274646 Msa0983910:intron 30.0%
! CAATCTATGAATGATTTTGC+AGG + chr6_4:27274763-27274782 Msa0983910:intron 30.0%
TCACCAAACATTTCAAAGAT+GGG + chr6_4:27274990-27275009 Msa0983910:CDS 30.0%
TAAGTTCAGTTTCTGAATGA+GGG - chr6_4:27275094-27275113 None:intergenic 30.0%
ATAAGTTCAGTTTCTGAATG+AGG - chr6_4:27275095-27275114 None:intergenic 30.0%
GAAATAGAAAACCATCATTG+AGG - chr6_4:27275361-27275380 None:intergenic 30.0%
AGATGGGGTTTCAAAAAAAG+AGG - chr6_4:27272738-27272757 None:intergenic 35.0%
TTGAGGAGATATGACGTTAT+TGG - chr6_4:27272801-27272820 None:intergenic 35.0%
! ATCCCACCATTTTACTTTGA+TGG + chr6_4:27272824-27272843 Msa0983910:CDS 35.0%
TGAAGTGGTTGTTGTAATGA+GGG - chr6_4:27272993-27273012 None:intergenic 35.0%
ATGAAGTGGTTGTTGTAATG+AGG - chr6_4:27272994-27273013 None:intergenic 35.0%
GGTTTGGATAATAGTGATTG+GGG - chr6_4:27273017-27273036 None:intergenic 35.0%
GGGTTTGGATAATAGTGATT+GGG - chr6_4:27273018-27273037 None:intergenic 35.0%
GTTGGACAATCAAAAAAGGT+GGG - chr6_4:27273118-27273137 None:intergenic 35.0%
TGTTGGACAATCAAAAAAGG+TGG - chr6_4:27273119-27273138 None:intergenic 35.0%
!! TCTTGTTGTTCGATAAGTGT+TGG - chr6_4:27273136-27273155 None:intergenic 35.0%
TTGAAATTATTGGTAGGGGT+GGG - chr6_4:27273217-27273236 None:intergenic 35.0%
ATTGAAATTATTGGTAGGGG+TGG - chr6_4:27273218-27273237 None:intergenic 35.0%
!! GGTGGGAGTATTGAAATTAT+TGG - chr6_4:27273227-27273246 None:intergenic 35.0%
!! GATAATGTTAGTCAAGTTGG+TGG - chr6_4:27273368-27273387 None:intergenic 35.0%
!! ATTGCATACTCTGAAATGGT+AGG - chr6_4:27273418-27273437 None:intergenic 35.0%
! GAGTATTGCATACTCTGAAA+TGG - chr6_4:27273422-27273441 None:intergenic 35.0%
TGATTGATTGAGCTTTCTTG+GGG - chr6_4:27273527-27273546 None:intergenic 35.0%
GTGATTGATTGAGCTTTCTT+GGG - chr6_4:27273528-27273547 None:intergenic 35.0%
TGTGATTGATTGAGCTTTCT+TGG - chr6_4:27273529-27273548 None:intergenic 35.0%
AAAATCCTAGATCTGGTGTT+GGG - chr6_4:27273562-27273581 None:intergenic 35.0%
TAAAATCCTAGATCTGGTGT+TGG - chr6_4:27273563-27273582 None:intergenic 35.0%
ATAAGCTTTCAGAAGATTGG+TGG + chr6_4:27273775-27273794 Msa0983910:CDS 35.0%
! TTTTGGATGTGAAAAGACCT+TGG - chr6_4:27273846-27273865 None:intergenic 35.0%
! AGACCACTATTTAAAACCCT+AGG - chr6_4:27273941-27273960 None:intergenic 35.0%
! CTGAATTGTGGTCTTAATCT+AGG + chr6_4:27273987-27274006 Msa0983910:intron 35.0%
ACAAAAAAGTGACACCAAAG+AGG + chr6_4:27274836-27274855 Msa0983910:CDS 35.0%
!! ACTTTTGTTGTGACGTTTGT+TGG + chr6_4:27274861-27274880 Msa0983910:CDS 35.0%
!!! ATTTACCTGCTGGTTTTTCA+TGG - chr6_4:27274896-27274915 None:intergenic 35.0%
ATGAAAAACCAGCAGGTAAA+TGG + chr6_4:27274895-27274914 Msa0983910:CDS 35.0%
!! AGTCTTTGATGACTTGTTCT+GGG - chr6_4:27274940-27274959 None:intergenic 35.0%
!! TAGTCTTTGATGACTTGTTC+TGG - chr6_4:27274941-27274960 None:intergenic 35.0%
GTCACCAAACATTTCAAAGA+TGG + chr6_4:27274989-27275008 Msa0983910:CDS 35.0%
!! GAACCCATCTTTGAAATGTT+TGG - chr6_4:27274996-27275015 None:intergenic 35.0%
! TAAGTTTGTTTCCTGAAGGA+AGG - chr6_4:27275256-27275275 None:intergenic 35.0%
! CCAATAAGTTTGTTTCCTGA+AGG - chr6_4:27275260-27275279 None:intergenic 35.0%
CCTTCAGGAAACAAACTTAT+TGG + chr6_4:27275257-27275276 Msa0983910:CDS 35.0%
TCTAACATATCAGTCATGCT+TGG - chr6_4:27275314-27275333 None:intergenic 35.0%
AAGCATGACTGATATGTTAG+AGG + chr6_4:27275313-27275332 Msa0983910:CDS 35.0%
ATAGAAAACCATCATTGAGG+TGG - chr6_4:27275358-27275377 None:intergenic 35.0%
TGGGGTTTCAAAAAAAGAGG+CGG - chr6_4:27272735-27272754 None:intergenic 40.0%
! TTGGTGTTTGAGATGGAGAT+GGG - chr6_4:27272754-27272773 None:intergenic 40.0%
! ATTGGTGTTTGAGATGGAGA+TGG - chr6_4:27272755-27272774 None:intergenic 40.0%
! ACAGTGATTGGTGTTTGAGA+TGG - chr6_4:27272761-27272780 None:intergenic 40.0%
AGATATGACGTTATTGGTGG+TGG - chr6_4:27272795-27272814 None:intergenic 40.0%
AGGAGATATGACGTTATTGG+TGG - chr6_4:27272798-27272817 None:intergenic 40.0%
TAAGTGGGGAAAAAGGAACA+GGG - chr6_4:27272874-27272893 None:intergenic 40.0%
TTAAGTGGGGAAAAAGGAAC+AGG - chr6_4:27272875-27272894 None:intergenic 40.0%
TGGTTGTTGTAATGAGGGTT+GGG - chr6_4:27272988-27273007 None:intergenic 40.0%
AATAGTGATTGGGGATGAAG+TGG - chr6_4:27273008-27273027 None:intergenic 40.0%
GGGGTTTGGATAATAGTGAT+TGG - chr6_4:27273019-27273038 None:intergenic 40.0%
TGTACCAAATGTTATGTGGC+CGG - chr6_4:27273059-27273078 None:intergenic 40.0%
ATCACTAACACTAGTGCTCA+TGG + chr6_4:27273079-27273098 Msa0983910:CDS 40.0%
TGAAATTATTGGTAGGGGTG+GGG - chr6_4:27273216-27273235 None:intergenic 40.0%
GTAATTCTAGTATGGGTGGA+AGG - chr6_4:27273259-27273278 None:intergenic 40.0%
CATGGGGGTAATTCTAGTAT+GGG - chr6_4:27273266-27273285 None:intergenic 40.0%
TCATGGGGGTAATTCTAGTA+TGG - chr6_4:27273267-27273286 None:intergenic 40.0%
GCGTGAAAAAAGTTGTTGTG+GGG - chr6_4:27273312-27273331 None:intergenic 40.0%
GGCGTGAAAAAAGTTGTTGT+GGG - chr6_4:27273313-27273332 None:intergenic 40.0%
!! AATGTTAGTCAAGTTGGTGG+TGG - chr6_4:27273365-27273384 None:intergenic 40.0%
AATTACCCAACACCAGATCT+AGG + chr6_4:27273554-27273573 Msa0983910:CDS 40.0%
TGTGAAAAGACCTTGGATGT+GGG - chr6_4:27273839-27273858 None:intergenic 40.0%
ATGTGAAAAGACCTTGGATG+TGG - chr6_4:27273840-27273859 None:intergenic 40.0%
AAGACCACAATTCAGTGCAA+TGG - chr6_4:27273982-27274001 None:intergenic 40.0%
!! CACAACAAAAGTGTCCTCTT+TGG - chr6_4:27274853-27274872 None:intergenic 40.0%
ATAGAGTTCCATTTACCTGC+TGG - chr6_4:27274906-27274925 None:intergenic 40.0%
GCAGGTAAATGGAACTCTAT+TGG + chr6_4:27274906-27274925 Msa0983910:CDS 40.0%
GGTAAATGGAACTCTATTGG+TGG + chr6_4:27274909-27274928 Msa0983910:CDS 40.0%
ACTTGAATATTCCTGCTCTC+TGG - chr6_4:27275050-27275069 None:intergenic 40.0%
TTCTCTAGCAGCAGGTAAAT+GGG - chr6_4:27275123-27275142 None:intergenic 40.0%
TTTCTCTAGCAGCAGGTAAA+TGG - chr6_4:27275124-27275143 None:intergenic 40.0%
GATTCAATTTCTCTAGCAGC+AGG - chr6_4:27275131-27275150 None:intergenic 40.0%
AGGAAGGATTTGTGATTCAC+CGG - chr6_4:27275240-27275259 None:intergenic 40.0%
GAATCACAAATCCTTCCTTC+AGG + chr6_4:27275242-27275261 Msa0983910:CDS 40.0%
!! GATTTTGACCACCTCAATGA+TGG + chr6_4:27275347-27275366 Msa0983910:CDS 40.0%
! AAAAGAGGCGGGGTTAGTAA+AGG - chr6_4:27272723-27272742 None:intergenic 45.0%
GGGTTTCAAAAAAAGAGGCG+GGG - chr6_4:27272733-27272752 None:intergenic 45.0%
GGGGTTTCAAAAAAAGAGGC+GGG - chr6_4:27272734-27272753 None:intergenic 45.0%
! TGGTGTTTGAGATGGAGATG+GGG - chr6_4:27272753-27272772 None:intergenic 45.0%
!! TATGACGTTATTGGTGGTGG+AGG - chr6_4:27272792-27272811 None:intergenic 45.0%
AGGAACAGGGTTATCGTCTT+GGG - chr6_4:27272861-27272880 None:intergenic 45.0%
AAGGAACAGGGTTATCGTCT+TGG - chr6_4:27272862-27272881 None:intergenic 45.0%
ATAGTGGTGGTAGTTGGGAT+GGG - chr6_4:27272965-27272984 None:intergenic 45.0%
! TTGGGATAGTGGTGGTAGTT+GGG - chr6_4:27272970-27272989 None:intergenic 45.0%
TGTAATGAGGGTTGGGATAG+TGG - chr6_4:27272981-27273000 None:intergenic 45.0%
GTGGTTGTTGTAATGAGGGT+TGG - chr6_4:27272989-27273008 None:intergenic 45.0%
ATTCCCGGCCACATAACATT+TGG + chr6_4:27273052-27273071 Msa0983910:CDS 45.0%
GTACCAAATGTTATGTGGCC+GGG - chr6_4:27273058-27273077 None:intergenic 45.0%
!! AGTTGTTGTGGGGTTAGCTA+AGG - chr6_4:27273302-27273321 None:intergenic 45.0%
CGGCGTGAAAAAAGTTGTTG+TGG - chr6_4:27273314-27273333 None:intergenic 45.0%
AGCTTTCTTGGGGATTGTTG+TGG - chr6_4:27273517-27273536 None:intergenic 45.0%
! TTTCAGAAGATTGGTGGTCG+TGG + chr6_4:27273781-27273800 Msa0983910:CDS 45.0%
TGGTGGTCGTGGACAAAATA+TGG + chr6_4:27273792-27273811 Msa0983910:CDS 45.0%
GTGAAAAGACCTTGGATGTG+GGG - chr6_4:27273838-27273857 None:intergenic 45.0%
ATTCAGTGCAATGGCAAGGA+TGG - chr6_4:27273973-27273992 None:intergenic 45.0%
CACAATTCAGTGCAATGGCA+AGG - chr6_4:27273977-27273996 None:intergenic 45.0%
CTTGCCATTGCACTGAATTG+TGG + chr6_4:27273975-27273994 Msa0983910:intron 45.0%
CACAACCATGAAAAACCAGC+AGG + chr6_4:27274888-27274907 Msa0983910:CDS 45.0%
!! CTTTGATGACTTGTTCTGGG+AGG - chr6_4:27274937-27274956 None:intergenic 45.0%
AAGACTAGATTACCCGCTGT+TGG + chr6_4:27274954-27274973 Msa0983910:CDS 45.0%
TACCCGCTGTTGGAAATTTC+AGG + chr6_4:27274964-27274983 Msa0983910:CDS 45.0%
GACCTGAAATTTCCAACAGC+GGG - chr6_4:27274969-27274988 None:intergenic 45.0%
CGACCTGAAATTTCCAACAG+CGG - chr6_4:27274970-27274989 None:intergenic 45.0%
TGATTCACCGGAGTAAACCT+GGG - chr6_4:27275228-27275247 None:intergenic 45.0%
!! TAAAAAAAAATGTATTTATA+TGG + chr6_4:27274462-27274481 Msa0983910:intron 5.0%
TAGTGGTGGTAGTTGGGATG+GGG - chr6_4:27272964-27272983 None:intergenic 50.0%
! GATAGTGGTGGTAGTTGGGA+TGG - chr6_4:27272966-27272985 None:intergenic 50.0%
! GTTGGGATAGTGGTGGTAGT+TGG - chr6_4:27272971-27272990 None:intergenic 50.0%
! AATGAGGGTTGGGATAGTGG+TGG - chr6_4:27272978-27272997 None:intergenic 50.0%
TAGTGGTGGTTGAAGGGGTT+TGG - chr6_4:27273033-27273052 None:intergenic 50.0%
GGGAATAGTGGTGGTTGAAG+GGG - chr6_4:27273038-27273057 None:intergenic 50.0%
CGGGAATAGTGGTGGTTGAA+GGG - chr6_4:27273039-27273058 None:intergenic 50.0%
CCTTCAACCACCACTATTCC+CGG + chr6_4:27273037-27273056 Msa0983910:CDS 50.0%
TGTTATGTGGCCGGGAATAG+TGG - chr6_4:27273050-27273069 None:intergenic 50.0%
ATGGGTGGAAGGAGTAGTGA+GGG - chr6_4:27273248-27273267 None:intergenic 50.0%
TATGGGTGGAAGGAGTAGTG+AGG - chr6_4:27273249-27273268 None:intergenic 50.0%
GGGGGTAATTCTAGTATGGG+TGG - chr6_4:27273263-27273282 None:intergenic 50.0%
!! AAGGCGTTGGTAGTGGTCAT+GGG - chr6_4:27273283-27273302 None:intergenic 50.0%
!! TAAGGCGTTGGTAGTGGTCA+TGG - chr6_4:27273284-27273303 None:intergenic 50.0%
!! GTTAGCTAAGGCGTTGGTAG+TGG - chr6_4:27273290-27273309 None:intergenic 50.0%
CTAATAGGTGCAGTGGTGGT+CGG - chr6_4:27273334-27273353 None:intergenic 50.0%
!! TCAAGTTGGTGGTGGTGCTA+GGG - chr6_4:27273357-27273376 None:intergenic 50.0%
TCGTTTCAGGGAGAGAGAGT+TGG + chr6_4:27274639-27274658 Msa0983910:intron 50.0%
CGTTTCAGGGAGAGAGAGTT+GGG + chr6_4:27274640-27274659 Msa0983910:intron 50.0%
ATGTCGATCATCCAGAGAGC+AGG + chr6_4:27275036-27275055 Msa0983910:CDS 50.0%
! ACCTGGGCATTGCTACTCAT+TGG - chr6_4:27275212-27275231 None:intergenic 50.0%
AGCAATGCCCAGGTTTACTC+CGG + chr6_4:27275218-27275237 Msa0983910:CDS 50.0%
GTGATTCACCGGAGTAAACC+TGG - chr6_4:27275229-27275248 None:intergenic 50.0%
! GAGGTGGTGGTGACAGTGAT+TGG - chr6_4:27272773-27272792 None:intergenic 55.0%
!! GTTATTGGTGGTGGAGGTGG+TGG - chr6_4:27272786-27272805 None:intergenic 55.0%
!! GACGTTATTGGTGGTGGAGG+TGG - chr6_4:27272789-27272808 None:intergenic 55.0%
CAGGGTTATCGTCTTGGGCA+AGG - chr6_4:27272856-27272875 None:intergenic 55.0%
!! ATGGAGAGAGGTGGTGGGAT+TGG - chr6_4:27272939-27272958 None:intergenic 55.0%
AGTGGTGGTAGTTGGGATGG+GGG - chr6_4:27272963-27272982 None:intergenic 55.0%
CCGGGAATAGTGGTGGTTGA+AGG - chr6_4:27273040-27273059 None:intergenic 55.0%
TATGTGGCCGGGAATAGTGG+TGG - chr6_4:27273047-27273066 None:intergenic 55.0%
TTGGTAGGGGTGGGGTTAAC+AGG - chr6_4:27273208-27273227 None:intergenic 55.0%
GTGGAAGGAGTAGTGAGGGT+GGG - chr6_4:27273244-27273263 None:intergenic 55.0%
!! AGGCGTTGGTAGTGGTCATG+GGG - chr6_4:27273282-27273301 None:intergenic 55.0%
!! TGTGGGGTTAGCTAAGGCGT+TGG - chr6_4:27273296-27273315 None:intergenic 55.0%
AGGGCTAATAGGTGCAGTGG+TGG - chr6_4:27273338-27273357 None:intergenic 55.0%
GCTAGGGCTAATAGGTGCAG+TGG - chr6_4:27273341-27273360 None:intergenic 55.0%
! GTGGTGGTGCTAGGGCTAAT+AGG - chr6_4:27273349-27273368 None:intergenic 55.0%
!! GTCAAGTTGGTGGTGGTGCT+AGG - chr6_4:27273358-27273377 None:intergenic 55.0%
GCCAATGAGTAGCAATGCCC+AGG + chr6_4:27275208-27275227 Msa0983910:CDS 55.0%
TGGTAGTTGGGATGGGGGCA+TGG - chr6_4:27272958-27272977 None:intergenic 60.0%
GGTGGAAGGAGTAGTGAGGG+TGG - chr6_4:27273245-27273264 None:intergenic 60.0%
!! GGCGTTGGTAGTGGTCATGG+GGG - chr6_4:27273281-27273300 None:intergenic 60.0%
! TTAGCGGGTCCCCACATCCA+AGG + chr6_4:27273826-27273845 Msa0983910:CDS 60.0%
GGGGCATGGAGAGAGGTGGT+GGG - chr6_4:27272944-27272963 None:intergenic 65.0%
! GATGGGGGCATGGAGAGAGG+TGG - chr6_4:27272948-27272967 None:intergenic 65.0%
! TGGGATGGGGGCATGGAGAG+AGG - chr6_4:27272951-27272970 None:intergenic 65.0%
! GGGGGCATGGAGAGAGGTGG+TGG - chr6_4:27272945-27272964 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 27272665 27275385 27272665 ID=Msa0983910;Name=Msa0983910
chr6_4 mRNA 27272665 27275385 27272665 ID=Msa0983910-mRNA-1;Parent=Msa0983910;Name=Msa0983910-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|559
chr6_4 exon 27272665 27273626 27272665 ID=Msa0983910-mRNA-1:exon:7722;Parent=Msa0983910-mRNA-1
chr6_4 exon 27273731 27273847 27273731 ID=Msa0983910-mRNA-1:exon:7723;Parent=Msa0983910-mRNA-1
chr6_4 exon 27274785 27275385 27274785 ID=Msa0983910-mRNA-1:exon:7724;Parent=Msa0983910-mRNA-1
chr6_4 CDS 27272665 27273626 27272665 ID=Msa0983910-mRNA-1:cds;Parent=Msa0983910-mRNA-1
chr6_4 CDS 27273731 27273847 27273731 ID=Msa0983910-mRNA-1:cds;Parent=Msa0983910-mRNA-1
chr6_4 CDS 27274785 27275385 27274785 ID=Msa0983910-mRNA-1:cds;Parent=Msa0983910-mRNA-1
Gene Sequence

>Msa0983910

ATGGACATTGACGATTGGGATCTCTTTTCCTTTGTCAATAACAACAAAGCTACAACCTTTACTAACCCCGCCTCTTTTTTTGAAACCCCATCTCCATCTCAAACACCAATCACTGTCACCACCACCTCCACCACCAATAACGTCATATCTCCTCAAAATATCCCACCATTTTACTTTGATGGTTTTAGCCTTGCCCAAGACGATAACCCTGTTCCTTTTTCCCCACTTAAACAAAATGATTTCATAGATCTAGACAAATTGAAAGTCAATTCCAATCCCACCACCTCTCTCCATGCCCCCATCCCAACTACCACCACTATCCCAACCCTCATTACAACAACCACTTCATCCCCAATCACTATTATCCAAACCCCTTCAACCACCACTATTCCCGGCCACATAACATTTGGTACAATCACTAACACTAGTGCTCATGGTTTCAAAAAAAAACCCACCTTTTTTGATTGTCCAACACTTATCGAACAACAAGAAATGCAATCAAATGGGCACGAGCTAAATAATTTTATACTCAACAAAAATCCTGTTAACCCCACCCCTACCAATAATTTCAATACTCCCACCCTCACTACTCCTTCCACCCATACTAGAATTACCCCCATGACCACTACCAACGCCTTAGCTAACCCCACAACAACTTTTTTCACGCCGACCACCACTGCACCTATTAGCCCTAGCACCACCACCAACTTGACTAACATTATCACTAGTGTTCATGGAACCAATCAATATCCTACCATTTCAGAGTATGCAATACTCATCAAACAACAACAAATTCAACCAAACAATAACAGTCAAGTGTTTGATCATGCATACAACTATTCATTTGCTCCACAACAATCCCCAAGAAAGCTCAATCAATCACAAATTCAATTACCCAACACCAGATCTAGGATTTTACCTAATTCAGATTCACAAAAAGCAAAACATAATTCAAGAATCAGAAAAATTAACAACAAGGTAACTTTGGAATGCCATGTGAGAACAAATAAGCTTTCAGAAGATTGGTGGTCGTGGACAAAATATGGACAAAAAAACATTAGCGGGTCCCCACATCCAAGGAGCTACTTTAGATGTAGCAGTTCTAAAGATTGTTCAGCGAAAAAACTAGTACAAAAAAGTGACACCAAAGAGGACACTTTTGTTGTGACGTTTGTTGGAGCACACAACCATGAAAAACCAGCAGGTAAATGGAACTCTATTGGTGGAACCTCCCAGAACAAGTCATCAAAGACTAGATTACCCGCTGTTGGAAATTTCAGGTCGTCACCAAACATTTCAAAGATGGGTTCTCAAAATGTGACGATGATGCATGTCGATCATCCAGAGAGCAGGAATATTCAAGTTCTCTCTAGTCAATCAAAAATCCCTCATTCAGAAACTGAACTTATAGAGACCCATTTACCTGCTGCTAGAGAAATTGAATCATCCCCTAATGTTGAAAACTTGTTTTCATATGATGTGACGATGCTGCAGTTTGATGAGCCAATGAGTAGCAATGCCCAGGTTTACTCCGGTGAATCACAAATCCTTCCTTCAGGAAACAAACTTATTGGAAACTCTGATGACGATGAAGAAATCATGATACCAAGCATGACTGATATGTTAGAGGATTTCTTATTGGATTTTGACCACCTCAATGATGGTTTTCTATTTCCTTAG
Protein sequence

>Msa0983910

MDIDDWDLFSFVNNNKATTFTNPASFFETPSPSQTPITVTTTSTTNNVISPQNIPPFYFDGFSLAQDDNPVPFSPLKQNDFIDLDKLKVNSNPTTSLHAPIPTTTTIPTLITTTTSSPITIIQTPSTTTIPGHITFGTITNTSAHGFKKKPTFFDCPTLIEQQEMQSNGHELNNFILNKNPVNPTPTNNFNTPTLTTPSTHTRITPMTTTNALANPTTTFFTPTTTAPISPSTTTNLTNIITSVHGTNQYPTISEYAILIKQQQIQPNNNSQVFDHAYNYSFAPQQSPRKLNQSQIQLPNTRSRILPNSDSQKAKHNSRIRKINNKVTLECHVRTNKLSEDWWSWTKYGQKNISGSPHPRSYFRCSSSKDCSAKKLVQKSDTKEDTFVVTFVGAHNHEKPAGKWNSIGGTSQNKSSKTRLPAVGNFRSSPNISKMGSQNVTMMHVDHPESRNIQVLSSQSKIPHSETELIETHLPAAREIESSPNVENLFSYDVTMLQFDEPMSSNAQVYSGESQILPSGNKLIGNSDDDEEIMIPSMTDMLEDFLLDFDHLNDGFLFP*