Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0997750 | AES76575.1 | 71.852 | 135 | 17 | 1 | 133 | 267 | 36 | 149 | 2.15e-46 | 164 |
| Msa0997750 | AES76575.1 | 77.612 | 134 | 16 | 2 | 3 | 125 | 19 | 149 | 4.07e-51 | 176 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0997750 | MtrunA17_Chr4g0052061 | 55.285 | 123 | 35 | 2 | 175 | 277 | 28 | 150 | 5.42e-32 | 116 |
| Msa0997750 | MtrunA17_Chr4g0052061 | 48.421 | 95 | 30 | 1 | 68 | 143 | 64 | 158 | 2.53e-16 | 75.1 |
| Msa0997750 | MtrunA17_Chr4g0052081 | 48.718 | 117 | 34 | 4 | 177 | 267 | 30 | 146 | 3.07e-17 | 78.2 |
Find 63 sgRNAs with CRISPR-Local
Find 722 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATCAACATGCTCAAGA+AGG | 0.584063 | 6_4:+77829840 | Msa0997750:CDS |
| AAGAAACCTATATAATCAAG+AGG | 0.508847 | 6_4:+77829996 | Msa0997750:CDS |
| AAGGACAATGTGAATCATCG+AGG | 0.753335 | 6_4:+77829791 | Msa0997750:CDS |
| AAGGTGGAGGAAGTTGACAA+AGG | 0.605500 | 6_4:+77820425 | Msa0997750:CDS |
| AAGGTGGAGGAAGTTGACAA+AGG | 0.721445 | 6_4:+77829859 | Msa0997750:CDS |
| AATCAAACAAATCCCGTCCA+AGG | 0.598244 | 6_4:-77819585 | None:intergenic |
| AATCAAACAAATCCCGTCCA+AGG | 0.598244 | 6_4:-77828951 | None:intergenic |
| ACCTCCTTATATGCTGCAGA+AGG | 0.605430 | 6_4:+77819648 | Msa0997750:CDS |
| ACCTCCTTATGTGCTGCAGA+AGG | 0.585928 | 6_4:+77829014 | Msa0997750:CDS |
| ACCTTCTGCAGCACATAAGG+AGG | 0.613637 | 6_4:-77829015 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGACTGAAATTCGATATATT+TGG | - | chr6_4:77819550-77819569 | None:intergenic | 30.0% | |
| TGTTCATTTCTTGAAAACCA+AGG | - | chr6_4:77820161-77820180 | None:intergenic | 30.0% | |
| TTCTAAATCAAGATCAACAG+GGG | - | chr6_4:77820465-77820484 | None:intergenic | 30.0% | |
| CTTCTAAATCAAGATCAACA+GGG | - | chr6_4:77820466-77820485 | None:intergenic | 30.0% | |
| TCTTCTAAATCAAGATCAAC+AGG | - | chr6_4:77820467-77820486 | None:intergenic | 30.0% | |
| TCACACACACAAGATTATAT+AGG | - | chr6_4:77820735-77820754 | None:intergenic | 30.0% | |
| TCAACTATGTATGTAATGGT+TGG | + | chr6_4:77821319-77821338 | Msa0997750:intron | 30.0% | |
| TCAATTATGGTATCATGCTA+AGG | - | chr6_4:77821869-77821888 | None:intergenic | 30.0% | |
| TGTTGTAAATGAAGTGTAGT+TGG | + | chr6_4:77821924-77821943 | Msa0997750:intron | 30.0% | |
| ACCCATTACAGAAAAATCAT+AGG | - | chr6_4:77822026-77822045 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 77819454 | 77830089 | 77819454 | ID=Msa0997750;Name=Msa0997750 |
| chr6_4 | mRNA | 77819454 | 77830089 | 77819454 | ID=Msa0997750-mRNA-1;Parent=Msa0997750;Name=Msa0997750-mRNA-1;_AED=0.26;_eAED=0.27;_QI=0|0|0|0.85|0.16|0|7|0|305 |
| chr6_4 | exon | 77819454 | 77819461 | 77819454 | ID=Msa0997750-mRNA-1:exon:14709;Parent=Msa0997750-mRNA-1 |
| chr6_4 | exon | 77819477 | 77819669 | 77819477 | ID=Msa0997750-mRNA-1:exon:14710;Parent=Msa0997750-mRNA-1 |
| chr6_4 | exon | 77820299 | 77820352 | 77820299 | ID=Msa0997750-mRNA-1:exon:14711;Parent=Msa0997750-mRNA-1 |
| chr6_4 | exon | 77820419 | 77820569 | 77820419 | ID=Msa0997750-mRNA-1:exon:14712;Parent=Msa0997750-mRNA-1 |
| chr6_4 | exon | 77828911 | 77829035 | 77828911 | ID=Msa0997750-mRNA-1:exon:14713;Parent=Msa0997750-mRNA-1 |
| chr6_4 | exon | 77829668 | 77829731 | 77829668 | ID=Msa0997750-mRNA-1:exon:14714;Parent=Msa0997750-mRNA-1 |
| chr6_4 | exon | 77829767 | 77830089 | 77829767 | ID=Msa0997750-mRNA-1:exon:14715;Parent=Msa0997750-mRNA-1 |
| chr6_4 | CDS | 77819454 | 77819461 | 77819454 | ID=Msa0997750-mRNA-1:cds;Parent=Msa0997750-mRNA-1 |
| chr6_4 | CDS | 77819477 | 77819669 | 77819477 | ID=Msa0997750-mRNA-1:cds;Parent=Msa0997750-mRNA-1 |
| chr6_4 | CDS | 77820299 | 77820352 | 77820299 | ID=Msa0997750-mRNA-1:cds;Parent=Msa0997750-mRNA-1 |
| chr6_4 | CDS | 77820419 | 77820569 | 77820419 | ID=Msa0997750-mRNA-1:cds;Parent=Msa0997750-mRNA-1 |
| chr6_4 | CDS | 77828911 | 77829035 | 77828911 | ID=Msa0997750-mRNA-1:cds;Parent=Msa0997750-mRNA-1 |
| chr6_4 | CDS | 77829668 | 77829731 | 77829668 | ID=Msa0997750-mRNA-1:cds;Parent=Msa0997750-mRNA-1 |
| chr6_4 | CDS | 77829767 | 77830089 | 77829767 | ID=Msa0997750-mRNA-1:cds;Parent=Msa0997750-mRNA-1 |
| Gene Sequence |
>Msa0997750 ATGATAAACGATGATGGATACCTTATATATCAAATTATTCTTCGTGAAATAACGAATTCCAATCAAATCAACGAAGTACCAAATATATCGAATTTCAGTCGAATTGAATCGCATGTCCTTGGACGGGATTTGTTTGATTGTCATAGGGCTAGAGCTACTACATCTGGATGTCAAAATCAACCTCCTTATATGCTGCAGAAGGCTGTGACTGAGGAGTACCTTCATGTAGGACAATGTGAATCATCTAGACAAAATCTGAAGAAGGTGGAGGAAGTTGACAAAGGTAAAGTCTCTACTTCCCCTGTTGATCTTGATTTAGAAGAACTTCTAAAAGTGAAGGCTTCATTGAAAGAGCTCCATGGTGTCATTGAAGTATCAACATATTTGCTTCTCCTTTCAATGAAACTACCAAATATATCGAATTTCGATCGAATTGAATCGCATGTCCTTGGACGGGATTTGTTTGATTGTCATGGTGATAGAGCTACTACATCGGGAGGTCGAAATCAACCTCCTTATGTGCTGCAGAAGAAAGCAAGTGAATTATCAGTTCTTTGCGGTGCTAGAATTAGTATTGTTGGATTCACCTCTGCCGTTGCGACTAAGGAGTACCTTCATGAAGGACAATGTGAATCATCGAGGCAAAATGTTAAGCCTTCTCGAAATCAAAACATCAACATGCTCAAGAAGGTGGAGGAAGTTGACAAAGGTAAAGTCTCTATTTCCCTTGCTGATCTTGATTTAGAGGAACTTCTAAAAGTGAAGGCTTCATTGAAAGTGCTCCATGGTGACATTGAAGCAACAACATATTTGCTCTCCTTTCAAAGAAACCTATATAATCAAGAGGGTCAAGTAGATTGCAGCATACAAGTCTCATTGGTTCGGATTGTAAGGCTTGAGAAAAATACAATGTTCTGA |
| Protein sequence |
>Msa0997750 MINDDGYLIYQIILREITNSNQINEVPNISNFSRIESHVLGRDLFDCHRARATTSGCQNQPPYMLQKAVTEEYLHVGQCESSRQNLKKVEEVDKGKVSTSPVDLDLEELLKVKASLKELHGVIEVSTYLLLLSMKLPNISNFDRIESHVLGRDLFDCHGDRATTSGGRNQPPYVLQKKASELSVLCGARISIVGFTSAVATKEYLHEGQCESSRQNVKPSRNQNINMLKKVEEVDKGKVSISLADLDLEELLKVKASLKVLHGDIEATTYLLSFQRNLYNQEGQVDCSIQVSLVRIVRLEKNTMF* |
