AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999230 MtrunA17_Chr6g0485431 88.643 361 41 0 58 418 1 361 0.0 658
Msa0999230 MtrunA17_Chr1g0189391 45.075 335 152 7 115 418 1 334 1.96e-80 251
Msa0999230 MtrunA17_Chr7g0249311 41.353 133 76 2 160 292 342 472 5.34e-24 104
Msa0999230 MtrunA17_Chr7g0249311 41.333 75 42 2 212 286 180 252 7.77e-13 70.5
Msa0999230 MtrunA17_Chr5g0418191 57.333 75 31 1 197 271 306 379 4.92e-23 100
Msa0999230 MtrunA17_Chr5g0418191 40.385 104 51 5 183 282 119 215 1.57e-13 72.0
Msa0999230 MtrunA17_Chr7g0263531 34.868 152 94 3 137 288 291 437 6.31e-23 100
Msa0999230 MtrunA17_Chr7g0263531 46.667 75 38 2 212 286 190 262 6.86e-14 73.2
Msa0999230 MtrunA17_Chr3g0129321 35.606 132 77 3 141 272 55 178 2.56e-22 94.4
Msa0999230 MtrunA17_Chr4g0042831 56.000 75 32 1 197 271 264 337 3.01e-22 97.8
Msa0999230 MtrunA17_Chr4g0042831 45.833 72 37 2 205 275 138 208 7.64e-13 69.7
Msa0999230 MtrunA17_Chr7g0218081 54.667 75 33 1 197 271 396 469 6.86e-22 97.8
Msa0999230 MtrunA17_Chr7g0218081 48.387 62 30 2 210 271 227 286 5.55e-12 67.4
Msa0999230 MtrunA17_Chr7g0249301 55.263 76 33 1 196 271 277 351 1.88e-21 95.1
Msa0999230 MtrunA17_Chr7g0249301 41.558 77 43 2 210 286 61 135 1.42e-12 68.9
Msa0999230 MtrunA17_Chr4g0051881 49.383 81 40 1 197 277 164 243 2.00e-21 94.7
Msa0999230 MtrunA17_Chr7g0270191 43.182 88 49 1 198 285 132 218 2.24e-21 93.6
Msa0999230 MtrunA17_Chr5g0445311 34.211 152 96 2 120 271 276 423 6.06e-21 95.1
Msa0999230 MtrunA17_Chr3g0101131 37.179 156 82 5 131 271 320 474 1.02e-20 94.4
Msa0999230 MtrunA17_Chr1g0194481 46.835 79 41 1 197 275 144 221 1.14e-20 92.0
Msa0999230 MtrunA17_Chr5g0443131 48.148 81 41 1 197 277 143 222 1.81e-20 91.3
Msa0999230 MtrunA17_Chr3g0079691 45.631 103 55 1 169 271 453 554 1.02e-19 91.7
Msa0999230 MtrunA17_Chr3g0079691 46.154 65 33 2 212 276 281 343 9.83e-12 67.0
Msa0999230 MtrunA17_Chr4g0004911 52.632 76 35 1 197 272 322 396 1.89e-19 90.5
Msa0999230 MtrunA17_Chr4g0004911 44.615 65 35 1 212 276 185 248 4.47e-13 70.9
Msa0999230 MtrunA17_Chr2g0294231 44.444 81 44 1 197 277 189 268 3.05e-19 89.0
Msa0999230 MtrunA17_Chr3g0129361 48.611 72 36 1 197 268 130 200 3.55e-19 85.9
Msa0999230 MtrunA17_Chr8g0383351 47.945 73 36 2 197 268 145 216 7.09e-19 85.1
Msa0999230 MtrunA17_Chr5g0418781 48.718 78 36 2 197 271 110 186 7.73e-19 86.3
Msa0999230 MtrunA17_Chr4g0068541 44.872 78 42 1 198 275 195 271 1.12e-18 87.4
Msa0999230 MtrunA17_Chr7g0248721 50.685 73 34 2 196 268 126 196 1.64e-18 84.0
Msa0999230 MtrunA17_Chr7g0243891 43.878 98 54 1 174 271 337 433 2.82e-18 87.0
Msa0999230 MtrunA17_Chr7g0243891 38.750 80 47 2 207 286 212 289 1.26e-11 66.2
Msa0999230 MtrunA17_Chr5g0447701 35.714 126 80 1 146 271 187 311 2.94e-18 85.9
Msa0999230 MtrunA17_Chr8g0383251 46.753 77 40 1 198 274 95 170 3.10e-18 82.0
Msa0999230 MtrunA17_Chr5g0432161 48.193 83 42 1 189 271 399 480 3.80e-18 86.7
Msa0999230 MtrunA17_Chr5g0432161 43.750 80 43 2 209 288 242 319 8.44e-11 63.9
Msa0999230 MtrunA17_Chr3g0106491 52.000 75 35 1 197 271 351 424 6.85e-18 85.5
Msa0999230 MtrunA17_Chr3g0106491 42.105 76 42 2 202 277 189 262 3.61e-12 68.2
Msa0999230 MtrunA17_Chr8g0335811 47.222 72 37 1 197 268 140 210 1.45e-17 81.3
Msa0999230 MtrunA17_Chr7g0224801 50.000 68 33 1 201 268 83 149 2.73e-17 79.3
Msa0999230 MtrunA17_Chr1g0150801 47.297 74 38 1 195 268 86 158 4.31e-17 78.2
Msa0999230 MtrunA17_Chr1g0188941 46.667 75 39 1 197 271 37 110 5.10e-17 76.6
Msa0999230 MtrunA17_Chr8g0386241 46.479 71 37 1 198 268 90 159 6.66e-16 75.5
Msa0999230 MtrunA17_Chr3g0099641 40.517 116 55 5 191 305 232 334 8.62e-16 79.0
Msa0999230 MtrunA17_Chr4g0074001 44.444 81 44 1 197 277 345 424 2.03e-15 78.2
Msa0999230 MtrunA17_Chr7g0238921 55.172 58 25 1 211 268 124 180 3.20e-15 76.3
Msa0999230 MtrunA17_Chr4g0060271 46.053 76 39 2 198 272 242 316 7.35e-15 76.6
Msa0999230 MtrunA17_Chr2g0293481 34.127 126 68 4 168 285 230 348 8.80e-15 75.9
Msa0999230 MtrunA17_Chr8g0379461 49.206 63 30 2 211 272 76 137 1.16e-14 73.2
Msa0999230 MtrunA17_Chr2g0284321 51.613 62 28 2 211 271 35 95 1.22e-14 72.8
Msa0999230 MtrunA17_Chr7g0270831 46.667 75 38 2 198 271 203 276 1.49e-14 75.1
Msa0999230 MtrunA17_Chr8g0348311 47.561 82 38 4 204 283 147 225 1.55e-14 74.3
Msa0999230 MtrunA17_Chr1g0147881 49.254 67 32 2 212 277 263 328 2.67e-14 73.6
Msa0999230 MtrunA17_Chr5g0401821 34.821 112 60 3 167 277 166 265 3.36e-14 72.4
Msa0999230 MtrunA17_Chr7g0270051 46.250 80 35 3 199 271 35 113 3.93e-14 72.0
Msa0999230 MtrunA17_Chr7g0216891 30.978 184 102 6 106 272 105 280 4.00e-14 74.3
Msa0999230 MtrunA17_Chr2g0319961 50.794 63 29 2 211 272 80 141 4.30e-14 72.0
Msa0999230 MtrunA17_Chr4g0071521 37.000 100 50 3 176 274 221 308 4.40e-14 72.8
Msa0999230 MtrunA17_Chr2g0322391 46.667 75 38 2 198 271 336 409 4.47e-14 73.9
Msa0999230 MtrunA17_Chr2g0288871 45.333 75 39 2 198 271 292 365 5.27e-14 73.9
Msa0999230 MtrunA17_Chr7g0248691 47.619 84 40 3 207 289 150 230 5.31e-14 72.8
Msa0999230 MtrunA17_Chr3g0139021 39.806 103 49 3 167 268 281 371 6.38e-14 73.2
Msa0999230 MtrunA17_Chr1g0150281 38.947 95 52 4 180 269 105 198 6.52e-14 72.0
Msa0999230 MtrunA17_Chr8g0387501 39.583 96 48 4 212 306 130 216 1.45e-13 70.5
Msa0999230 MtrunA17_Chr4g0051181 45.000 80 42 2 194 272 226 304 2.30e-13 71.6
Msa0999230 MtrunA17_Chr2g0302391 46.154 78 40 2 212 288 212 288 3.39e-13 70.9
Msa0999230 MtrunA17_Chr2g0333851 40.260 77 42 3 198 271 154 229 4.16e-13 69.7
Msa0999230 MtrunA17_Chr4g0025061 42.500 80 44 2 198 276 347 425 6.29e-13 70.5
Msa0999230 MtrunA17_Chr4g0053281 35.714 98 50 3 174 270 240 325 8.29e-13 69.3
Msa0999230 MtrunA17_Chr8g0387981 43.750 80 36 3 195 266 111 189 1.16e-12 68.9
Msa0999230 MtrunA17_Chr7g0228941 51.724 58 26 2 212 268 53 109 2.08e-12 63.9
Msa0999230 MtrunA17_Chr1g0184691 50.847 59 27 2 212 269 294 351 2.14e-12 68.2
Msa0999230 MtrunA17_Chr1g0202461 45.556 90 44 4 212 299 262 348 2.61e-12 68.2
Msa0999230 MtrunA17_Chr1g0150271 36.364 77 45 3 198 271 86 161 2.82e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999230 AT5G41570.1 53.425 73 33 1 196 268 83 154 2.96e-22 93.6
Msa0999230 AT1G29860.1 56.757 74 31 1 198 271 123 195 3.05e-22 96.3
Msa0999230 AT4G18170.1 56.000 75 32 1 197 271 158 231 3.25e-22 97.1
Msa0999230 AT1G13960.1 54.667 75 33 1 197 271 395 468 3.58e-22 99.0
Msa0999230 AT1G13960.1 46.575 73 36 3 205 276 222 292 6.79e-13 70.9
Msa0999230 AT1G13960.2 54.667 75 33 1 197 271 242 315 4.59e-22 97.1
Msa0999230 AT1G13960.2 46.575 73 36 3 205 276 69 139 1.99e-12 68.6
Msa0999230 AT2G03340.1 54.667 75 33 1 197 271 401 474 8.53e-22 97.8
Msa0999230 AT2G03340.1 47.761 67 32 3 206 271 244 308 2.65e-12 68.9
Msa0999230 AT1G64000.1 53.521 71 32 1 198 268 101 170 1.00e-21 92.8
Msa0999230 AT5G46350.1 54.667 75 33 1 197 271 169 242 1.13e-21 95.5
Msa0999230 AT5G56270.1 38.194 144 85 3 128 271 407 546 1.69e-20 94.4
Msa0999230 AT5G56270.1 50.877 57 26 2 212 268 274 328 3.36e-11 65.9
Msa0999230 AT2G47260.1 47.436 78 40 1 198 275 161 237 3.09e-20 91.7
Msa0999230 AT1G69310.4 46.591 88 40 3 197 278 133 219 3.54e-20 90.5
Msa0999230 AT1G69310.2 46.591 88 40 3 197 278 133 219 3.54e-20 90.5
Msa0999230 AT1G69310.1 46.591 88 40 3 197 278 133 219 3.54e-20 90.5
Msa0999230 AT5G49520.1 49.333 75 37 1 198 272 208 281 4.18e-20 92.0
Msa0999230 AT3G01080.2 53.333 75 34 1 197 271 330 403 4.57e-20 92.4
Msa0999230 AT3G01080.2 48.387 62 30 2 210 271 204 263 8.31e-11 63.9
Msa0999230 AT3G01080.1 53.333 75 34 1 197 271 292 365 5.46e-20 92.0
Msa0999230 AT3G01080.1 48.387 62 30 2 210 271 166 225 9.29e-11 63.9
Msa0999230 AT2G46130.1 51.389 72 34 1 197 268 16 86 7.64e-20 84.7
Msa0999230 AT5G07100.2 54.667 75 33 1 197 271 127 200 1.55e-19 87.0
Msa0999230 AT5G07100.4 54.667 75 33 1 197 271 127 200 1.55e-19 87.0
Msa0999230 AT5G07100.5 54.667 75 33 1 197 271 127 200 1.55e-19 87.0
Msa0999230 AT5G07100.3 54.667 75 33 1 197 271 127 200 1.55e-19 87.0
Msa0999230 AT5G07100.1 54.667 75 33 1 197 271 220 293 1.56e-19 89.0
Msa0999230 AT5G07100.1 35.484 93 56 3 188 277 91 182 5.91e-11 63.5
Msa0999230 AT5G64810.1 32.847 137 84 2 132 268 38 166 3.62e-19 85.5
Msa0999230 AT1G55600.1 50.000 74 36 1 198 271 294 366 4.28e-19 89.7
Msa0999230 AT4G26640.7 52.000 75 35 1 197 271 327 400 9.53e-19 88.6
Msa0999230 AT4G26640.2 52.000 75 35 1 197 271 367 440 1.05e-18 88.6
Msa0999230 AT4G26640.1 52.000 75 35 1 197 271 295 368 1.18e-18 88.2
Msa0999230 AT4G26640.5 52.000 75 35 1 197 271 191 264 1.24e-18 87.4
Msa0999230 AT4G26640.6 52.000 75 35 1 197 271 191 264 1.24e-18 87.4
Msa0999230 AT4G26640.4 52.000 75 35 1 197 271 191 264 1.24e-18 87.4
Msa0999230 AT4G26640.3 52.000 75 35 1 197 271 191 264 1.24e-18 87.4
Msa0999230 AT2G04880.2 50.000 74 36 1 198 271 270 342 1.66e-18 87.8
Msa0999230 AT2G04880.2 40.217 92 51 3 205 294 105 194 3.22e-11 65.5
Msa0999230 AT2G04880.1 50.000 74 36 1 198 271 294 366 1.93e-18 87.8
Msa0999230 AT2G04880.1 39.216 102 56 4 205 302 105 204 4.23e-11 65.1
Msa0999230 AT2G38470.1 52.000 75 35 1 197 271 348 421 1.95e-18 87.8
Msa0999230 AT2G21900.1 44.156 77 39 2 200 273 95 170 2.11e-18 83.6
Msa0999230 AT2G37260.3 52.174 69 32 1 200 268 258 325 2.73e-18 85.9
Msa0999230 AT2G37260.3 36.220 127 56 5 212 338 86 187 2.72e-12 68.2
Msa0999230 AT4G39410.1 50.000 72 35 1 197 268 209 279 2.80e-18 85.5
Msa0999230 AT2G37260.2 52.174 69 32 1 200 268 256 323 2.89e-18 85.9
Msa0999230 AT2G37260.2 36.220 127 56 5 212 338 84 185 3.05e-12 67.8
Msa0999230 AT5G26170.1 45.070 71 38 1 198 268 100 169 2.99e-18 82.4
Msa0999230 AT2G37260.1 54.688 64 28 1 205 268 343 405 3.43e-18 86.7
Msa0999230 AT2G37260.1 36.220 127 56 5 212 338 166 267 2.82e-12 68.6
Msa0999230 AT2G30250.1 45.679 81 43 1 197 277 314 393 3.89e-18 86.3
Msa0999230 AT2G44745.1 47.222 72 37 1 197 268 131 201 6.44e-18 82.4
Msa0999230 AT3G62340.1 46.154 78 41 1 196 273 103 179 7.18e-18 83.6
Msa0999230 AT4G30935.1 52.113 71 33 1 212 282 332 401 4.05e-17 83.6
Msa0999230 AT5G13080.1 47.887 71 36 1 198 268 54 123 9.76e-17 77.4
Msa0999230 AT4G23550.1 39.496 119 60 6 194 304 124 238 2.50e-16 79.7
Msa0999230 AT4G26440.1 40.404 99 58 1 173 271 334 431 4.02e-16 80.9
Msa0999230 AT1G69310.3 43.182 88 39 4 197 278 133 215 1.41e-15 77.0
Msa0999230 AT1G29280.1 49.231 65 31 2 211 274 74 137 2.03e-15 76.3
Msa0999230 AT4G01250.1 42.991 107 47 5 211 304 128 233 2.08e-15 77.0
Msa0999230 AT2G46130.2 47.222 72 33 2 197 268 16 82 2.57e-15 72.0
Msa0999230 AT3G01970.1 41.667 72 41 1 198 269 52 122 1.31e-14 71.2
Msa0999230 AT1G30650.1 49.206 63 30 2 211 272 217 278 2.47e-14 74.7
Msa0999230 AT5G15130.2 41.935 93 44 3 198 289 172 255 2.78e-14 75.1
Msa0999230 AT2G34830.2 48.529 68 31 3 211 277 224 288 2.81e-14 74.7
Msa0999230 AT2G34830.1 48.529 68 31 3 211 277 215 279 2.92e-14 74.7
Msa0999230 AT5G15130.1 41.237 97 47 3 198 293 214 301 2.93e-14 75.1
Msa0999230 AT1G62300.1 48.000 75 37 2 198 271 299 372 3.17e-14 75.1
Msa0999230 AT3G58710.1 44.578 83 42 3 211 290 71 152 4.31e-14 72.4
Msa0999230 AT4G04450.2 37.615 109 64 3 164 271 153 258 4.54e-14 73.9
Msa0999230 AT3G58710.2 44.578 83 42 3 211 290 70 151 4.55e-14 72.4
Msa0999230 AT1G68150.1 41.304 92 52 2 194 284 218 308 4.69e-14 73.6
Msa0999230 AT4G31550.2 39.216 102 49 3 168 268 213 302 5.42e-14 73.2
Msa0999230 AT2G25000.2 46.032 63 32 2 210 271 102 163 6.29e-14 71.2
Msa0999230 AT2G25000.4 46.032 63 32 2 210 271 102 163 6.29e-14 71.2
Msa0999230 AT2G25000.3 46.032 63 32 2 210 271 124 185 9.71e-14 71.2
Msa0999230 AT4G04450.1 37.615 109 64 3 164 271 247 352 1.48e-13 72.8
Msa0999230 AT2G25000.1 46.032 63 32 2 210 271 145 206 1.50e-13 70.9
Msa0999230 AT4G22070.4 46.667 75 38 2 198 271 284 357 1.72e-13 72.8
Msa0999230 AT4G22070.3 46.667 75 38 2 198 271 284 357 1.72e-13 72.8
Msa0999230 AT4G22070.2 46.667 75 38 2 198 271 284 357 1.72e-13 72.8
Msa0999230 AT4G22070.1 46.667 75 38 2 198 271 284 357 1.72e-13 72.8
Msa0999230 AT2G23320.1 51.667 60 27 2 212 270 241 299 2.69e-13 70.9
Msa0999230 AT2G30590.1 53.448 58 25 2 212 268 314 370 2.91e-13 71.2
Msa0999230 AT1G18860.1 26.214 206 130 7 84 271 98 299 3.44e-13 71.6
Msa0999230 AT4G31550.1 53.448 58 25 2 212 268 247 303 3.62e-13 70.5
Msa0999230 AT5G52830.1 42.308 104 49 4 207 309 161 254 6.34e-13 70.1
Msa0999230 AT4G24240.1 36.697 109 62 4 172 274 237 344 7.92e-13 69.7
Msa0999230 AT4G11070.2 31.944 144 78 6 183 308 76 217 9.28e-13 68.9
Msa0999230 AT5G43290.1 40.476 84 48 2 211 293 114 196 9.79e-13 68.6
Msa0999230 AT4G31800.2 44.444 63 33 2 210 271 110 171 1.01e-12 68.2
Msa0999230 AT4G31800.1 44.444 63 33 2 210 271 175 236 1.41e-12 68.6
Msa0999230 AT4G11070.1 31.944 144 78 6 183 308 108 249 1.44e-12 68.6
Msa0999230 AT5G28650.1 41.284 109 57 5 163 268 215 319 2.42e-12 68.2
Msa0999230 AT2G24570.1 51.724 58 26 2 212 268 244 300 7.39e-12 66.6
Msa0999230 AT1G80840.1 41.270 63 35 2 210 271 145 206 8.61e-12 66.2
Msa0999230 AT5G45050.4 46.875 64 32 2 211 273 1041 1103 1.21e-11 67.4
Msa0999230 AT5G45050.1 46.875 64 32 2 211 273 1180 1242 1.25e-11 67.4
Msa0999230 AT5G45050.2 46.875 64 32 2 211 273 1152 1214 1.27e-11 67.4
Msa0999230 AT3G04670.1 51.724 58 26 2 212 268 263 319 1.59e-11 65.5
Msa0999230 AT5G24110.1 47.541 61 28 2 210 267 112 171 1.93e-11 65.1

Find 89 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAACTTGTTGTTCATCTA+AGG 0.247246 6_4:-81028071 None:intergenic
GGAAAGGAAATATTTGAAAT+TGG 0.258518 6_4:-81027875 None:intergenic
CATCAGCTGCAACAACTTCT+TGG 0.283729 6_4:-81028033 None:intergenic
TATGAAACAATTACATATTT+CGG 0.286298 6_4:-81029173 None:intergenic
ATGCCTTATGGATATATTAA+TGG 0.289062 6_4:+81029434 Msa0999230:CDS
TATTTGAAATCCACCAATAC+TGG 0.290258 6_4:-81029513 None:intergenic
TATATATACCTGAGAATAAT+CGG 0.300770 6_4:-81026442 None:intergenic
TTCAAGAAAGGATTATTATT+AGG 0.304221 6_4:-81029389 None:intergenic
TCAATTCTGAACACTACTTT+TGG 0.317569 6_4:-81028345 None:intergenic
GAGTTGATTGCTCGGCTATA+AGG 0.347334 6_4:+81026373 Msa0999230:CDS
CGAAGAAAATCATGAAGTTC+TGG 0.347870 6_4:-81029548 None:intergenic
AATACCATGATGATGCCTTA+TGG 0.355278 6_4:+81029422 Msa0999230:CDS
GGAAACCAAGTACTAAATTC+TGG 0.369352 6_4:+81026351 Msa0999230:CDS
TCTGAAAGTTATGAATCTTA+TGG 0.371782 6_4:+81029350 Msa0999230:CDS
GGTTATCGTTGGCATAAGTA+TGG 0.380213 6_4:+81028393 Msa0999230:CDS
CCTAAATATCATTCATTACT+AGG 0.380869 6_4:-81029653 None:intergenic
TCGTTGGCTTCATGATTAAT+TGG 0.383142 6_4:-81029626 None:intergenic
AGTGTAGTACTTGACTTTGT+TGG 0.385433 6_4:-81029242 None:intergenic
TATGTAATTGTTTCATATGA+AGG 0.393620 6_4:+81029179 Msa0999230:CDS
ATCCATTAGGCTGTTGTTGA+TGG 0.395630 6_4:+81027800 Msa0999230:intron
CAAGTTACAAATACAACTAT+TGG 0.398555 6_4:+81028085 Msa0999230:CDS
AAGCAACATCATTCCTTCAA+TGG 0.416911 6_4:-81028122 None:intergenic
CAACTCCAGAATTTAGTACT+TGG 0.423817 6_4:-81026356 None:intergenic
TTGGATGTTGAACTTCATCT+TGG 0.424960 6_4:-81027856 None:intergenic
GATGGTTTCCACATGGCAAC+GGG 0.430688 6_4:+81027818 Msa0999230:CDS
TGTGAGAAAACAGGTGGAAA+GGG 0.431043 6_4:+81029139 Msa0999230:CDS
TCCTTCTCCCGATTATTCTC+AGG 0.437382 6_4:+81026434 Msa0999230:CDS
TTCCACATGGCAACGGGAAG+AGG 0.449038 6_4:+81027824 Msa0999230:CDS
GCATCTTCACGATAGTTCAT+TGG 0.451177 6_4:-81028004 None:intergenic
CAAGCTGTTCCCAGTATTGG+TGG 0.453491 6_4:+81029503 Msa0999230:CDS
ATCAGCTGCAACAACTTCTT+GGG 0.455443 6_4:-81028032 None:intergenic
TCTCAAGCTGTTCCCAGTAT+TGG 0.455665 6_4:+81029500 Msa0999230:CDS
GGTGAGGAGGTGTGAAAGGT+TGG 0.471015 6_4:-81029325 None:intergenic
GGTGTGAAAGGTTGGGTCAT+AGG 0.474536 6_4:-81029317 None:intergenic
TTATTAACCATGTTCAAGAA+AGG 0.482422 6_4:-81029401 None:intergenic
TTTCCAACAACACTTGATCA+AGG 0.493003 6_4:+81027893 Msa0999230:CDS
GCTTGAGAGGCAGCAATTGA+AGG 0.494366 6_4:-81029485 None:intergenic
TTGATCAAGTGTTGTTGGAA+AGG 0.495068 6_4:-81027891 None:intergenic
ATTGGTGTAGCACCTTCAAA+AGG 0.502923 6_4:-81029608 None:intergenic
CTTATGGTAAGAACTGTACT+TGG 0.502973 6_4:+81029366 Msa0999230:CDS
GTTGTTGTTGTCGTCGTCCA+TGG 0.504429 6_4:-81026316 None:intergenic
TAATAATCCTTTCTTGAACA+TGG 0.506250 6_4:+81029394 Msa0999230:CDS
GTACTTGATCGATTTAAATG+AGG 0.506847 6_4:+81026407 Msa0999230:CDS
TGTCCTTGATCAAGTGTTGT+TGG 0.508287 6_4:-81027896 None:intergenic
GTTGAAGCAAGTTCCATTGA+AGG 0.510335 6_4:+81028109 Msa0999230:CDS
GCTAAACGCTGAAATTCTAA+AGG 0.517982 6_4:+81027964 Msa0999230:CDS
CATAATGATACTAGTGCTAG+TGG 0.520978 6_4:+81028148 Msa0999230:CDS
AACCTGTAATGTGAGAAAAC+AGG 0.528589 6_4:+81029130 Msa0999230:CDS
AATGAAGACAAAGTGAATGA+TGG 0.529985 6_4:+81028372 Msa0999230:CDS
GTGAGGAGGTGTGAAAGGTT+GGG 0.531143 6_4:-81029324 None:intergenic
CACCTATATGTCTCATACCA+TGG 0.531735 6_4:+81026299 None:intergenic
ATGTGAGAAAACAGGTGGAA+AGG 0.535021 6_4:+81029138 Msa0999230:CDS
AAATCATGAAGTTCTGGTAG+TGG 0.536148 6_4:-81029542 None:intergenic
TGATGGTTTCCACATGGCAA+CGG 0.539308 6_4:+81027817 Msa0999230:CDS
ATATATACCTGAGAATAATC+GGG 0.550091 6_4:-81026441 None:intergenic
TGTCCATTAATATATCCATA+AGG 0.552023 6_4:-81029437 None:intergenic
AATACTGGGAACAGCTTGAG+AGG 0.558169 6_4:-81029498 None:intergenic
TCATGAAGTTCTGGTAGTGG+TGG 0.567685 6_4:-81029539 None:intergenic
GAGCGGTGAGGAGGTGTGAA+AGG 0.568345 6_4:-81029329 None:intergenic
TTCATTACTAGGACTGTCGT+TGG 0.572830 6_4:-81029642 None:intergenic
AAATTCTGGAGTTGATTGCT+CGG 0.577016 6_4:+81026365 Msa0999230:CDS
TGGGTCATAGGCAATGCAAA+AGG 0.578767 6_4:-81029305 None:intergenic
AAGGAAATGCTAAATACCCT+AGG 0.580382 6_4:+81028430 Msa0999230:CDS
ATTTGAAATCCACCAATACT+GGG 0.584480 6_4:-81029512 None:intergenic
GCAACATTTATCATGGTGCA+AGG 0.584736 6_4:-81028321 None:intergenic
GAAATTCTAAAGGCTAGGAA+AGG 0.587867 6_4:+81027974 Msa0999230:CDS
ACGCTGAAATTCTAAAGGCT+AGG 0.591343 6_4:+81027969 Msa0999230:CDS
TTGATTGCTCGGCTATAAGG+AGG 0.594643 6_4:+81026376 Msa0999230:CDS
ACCTGAGAATAATCGGGAGA+AGG 0.596196 6_4:-81026435 None:intergenic
TAATTGCATTACTTTGCACA+TGG 0.597072 6_4:-81027940 None:intergenic
TATGGACGCAAGGAGATCAA+AGG 0.600725 6_4:+81028411 Msa0999230:CDS
TGCATTATAGGGGATACTAC+AGG 0.601431 6_4:+81029096 Msa0999230:intron
CACCTCTTCCCGTTGCCATG+TGG 0.601831 6_4:-81027826 None:intergenic
GCTGCAACAACTTCTTGGGT+AGG 0.614907 6_4:-81028028 None:intergenic
TTGGCATAAGTATGGACGCA+AGG 0.617304 6_4:+81028401 Msa0999230:CDS
CTAGTGGATCTTCTTCACAA+AGG 0.623228 6_4:+81028164 Msa0999230:CDS
TAACTTTCAGAGCGGTGAGG+AGG 0.625278 6_4:-81029338 None:intergenic
TATGAAGGCAAACATAATCA+CGG 0.626212 6_4:+81029194 Msa0999230:CDS
AAGTGAATGATGGTTATCGT+TGG 0.626452 6_4:+81028382 Msa0999230:CDS
GTCATAGGCAATGCAAAAGG+TGG 0.645286 6_4:-81029302 None:intergenic
CTGTAATGTGAGAAAACAGG+TGG 0.647364 6_4:+81029133 Msa0999230:CDS
CAACATTTATCATGGTGCAA+GGG 0.652471 6_4:-81028320 None:intergenic
ATATCCATAAGGCATCATCA+TGG 0.655849 6_4:-81029426 None:intergenic
TGATTGCTCGGCTATAAGGA+GGG 0.663653 6_4:+81026377 Msa0999230:CDS
TGTTGTTGATGGTTTCCACA+TGG 0.683427 6_4:+81027811 Msa0999230:CDS
ACAACACTTGATCAAGGACA+TGG 0.685805 6_4:+81027899 Msa0999230:CDS
TCATAACTTTCAGAGCGGTG+AGG 0.687777 6_4:-81029341 None:intergenic
AAGATTCATAACTTTCAGAG+CGG 0.728852 6_4:-81029346 None:intergenic
CACTGACAACAGTAACACGT+AGG 0.735467 6_4:+81029261 Msa0999230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTAAATAATAAATTT+TGG - chr6_4:81028922-81028941 None:intergenic 0.0%
!! TATTATTATATAATACATGT+TGG - chr6_4:81026787-81026806 None:intergenic 10.0%
!!! ATATAAATTTTACTTTTCAA+GGG - chr6_4:81026897-81026916 None:intergenic 10.0%
!!! AATATAAATTTTACTTTTCA+AGG - chr6_4:81026898-81026917 None:intergenic 10.0%
!!! TATTATATTTGCTATATTAT+TGG - chr6_4:81027366-81027385 None:intergenic 10.0%
!!! AAATTTGGATTAGTTTATAA+TGG - chr6_4:81026600-81026619 None:intergenic 15.0%
!! AATTTCCTATTGAAAAATAA+TGG - chr6_4:81026679-81026698 None:intergenic 15.0%
!! AAATATTTAAACATGACAAA+CGG + chr6_4:81026735-81026754 Msa0999230:intron 15.0%
!! AGTGCATATTTAATTATTAA+GGG - chr6_4:81026957-81026976 None:intergenic 15.0%
!! TAGTGCATATTTAATTATTA+AGG - chr6_4:81026958-81026977 None:intergenic 15.0%
!!! ATATTTTTATGATACATAAG+AGG + chr6_4:81027005-81027024 Msa0999230:intron 15.0%
!!! TATTTTTATGATACATAAGA+GGG + chr6_4:81027006-81027025 Msa0999230:intron 15.0%
!!! TAAAAATGTTACATTGTTTT+TGG - chr6_4:81027505-81027524 None:intergenic 15.0%
!!! AAACAATGTAACATTTTTAT+AGG + chr6_4:81027506-81027525 Msa0999230:intron 15.0%
!! TTTATAGGTAAATTTATTGA+AGG + chr6_4:81027521-81027540 Msa0999230:intron 15.0%
!! TTATAGGTAAATTTATTGAA+GGG + chr6_4:81027522-81027541 Msa0999230:intron 15.0%
!! TAGTATAAATTATAGTTTCA+TGG + chr6_4:81027594-81027613 Msa0999230:intron 15.0%
!! AGTATAAATTATAGTTTCAT+GGG + chr6_4:81027595-81027614 Msa0999230:intron 15.0%
!! TAAAAAAAATCATTTGATGA+TGG - chr6_4:81027650-81027669 None:intergenic 15.0%
!! TATTTGTTTCAAACAAAAAA+TGG + chr6_4:81028216-81028235 Msa0999230:intron 15.0%
!! AAACTTCTAATTATAAAGAA+CGG - chr6_4:81028628-81028647 None:intergenic 15.0%
!! CAATTTGATATTAAATTTGT+GGG + chr6_4:81028856-81028875 Msa0999230:intron 15.0%
!! ATTAATATTGTATGCATTAT+AGG + chr6_4:81029084-81029103 Msa0999230:intron 15.0%
!! TTAATATTGTATGCATTATA+GGG + chr6_4:81029085-81029104 Msa0999230:intron 15.0%
!! TATGAAACAATTACATATTT+CGG - chr6_4:81029176-81029195 None:intergenic 15.0%
!! TATATATACCTGAGAATAAT+CGG - chr6_4:81026445-81026464 None:intergenic 20.0%
!!! TGTATATTTTAACATAAGAG+GGG - chr6_4:81027110-81027129 None:intergenic 20.0%
!!! GTGTATATTTTAACATAAGA+GGG - chr6_4:81027109-81027128 None:intergenic 20.0%
!!! AGTGTATATTTTAACATAAG+AGG - chr6_4:81027108-81027127 None:intergenic 20.0%
!! TATTTAATTATTAAGGGAGA+GGG - chr6_4:81026951-81026970 None:intergenic 20.0%
!! ATATTTAATTATTAAGGGAG+AGG - chr6_4:81026952-81026971 None:intergenic 20.0%
!!! ATTTTTATGATACATAAGAG+GGG + chr6_4:81027007-81027026 Msa0999230:intron 20.0%
!!! TCTAATATGTTTTGAATAAG+AGG + chr6_4:81027078-81027097 Msa0999230:intron 20.0%
!!! CTAATATGTTTTGAATAAGA+GGG + chr6_4:81027079-81027098 Msa0999230:intron 20.0%
!!! TAATATGTTTTGAATAAGAG+GGG + chr6_4:81027080-81027099 Msa0999230:intron 20.0%
!!! AGTGTATATTTTAACATAAG+AGG + chr6_4:81027108-81027127 Msa0999230:intron 20.0%
!!! GTGTATATTTTAACATAAGA+GGG + chr6_4:81027109-81027128 Msa0999230:intron 20.0%
!!! TGTATATTTTAACATAAGAG+GGG + chr6_4:81027110-81027129 Msa0999230:intron 20.0%
!! CTACATATAAAAAGAATACA+TGG - chr6_4:81027577-81027596 None:intergenic 20.0%
!! AACTTCTAATTATAAAGAAC+GGG - chr6_4:81028627-81028646 None:intergenic 20.0%
!!! TATACTTTGAGATGATTTTA+AGG + chr6_4:81028780-81028799 Msa0999230:intron 20.0%
!! GCAATTTGATATTAAATTTG+TGG + chr6_4:81028855-81028874 Msa0999230:intron 20.0%
!! ATGATGTAAATATTGTTCAT+TGG + chr6_4:81029005-81029024 Msa0999230:intron 20.0%
!! AATATTGTTCATTGGAATTA+AGG + chr6_4:81029013-81029032 Msa0999230:intron 20.0%
!! ATATTTATGTATATGCTACA+TGG - chr6_4:81029047-81029066 None:intergenic 20.0%
!! TAATATTGTATGCATTATAG+GGG + chr6_4:81029086-81029105 Msa0999230:intron 20.0%
!! TATGTAATTGTTTCATATGA+AGG + chr6_4:81029179-81029198 Msa0999230:CDS 20.0%
!!! GATTTTTTTTTGTGATGATA+GGG - chr6_4:81029223-81029242 None:intergenic 20.0%
!! TTCAAGAAAGGATTATTATT+AGG - chr6_4:81029392-81029411 None:intergenic 20.0%
! ATATATACCTGAGAATAATC+GGG - chr6_4:81026444-81026463 None:intergenic 25.0%
!!! TTGATCATTTTTTGTTGCAT+TGG - chr6_4:81026481-81026500 None:intergenic 25.0%
! GGTAGAAAATGATGTAAATT+TGG - chr6_4:81026615-81026634 None:intergenic 25.0%
!! TTTTAATAATCACATAAGCG+AGG - chr6_4:81026639-81026658 None:intergenic 25.0%
!!! AAATTTTACTTTTCAAGGGA+GGG - chr6_4:81026893-81026912 None:intergenic 25.0%
!!! TAAATTTTACTTTTCAAGGG+AGG - chr6_4:81026894-81026913 None:intergenic 25.0%
! GTAATTCAAGCATCTTATAA+GGG - chr6_4:81026926-81026945 None:intergenic 25.0%
! TGTAATTCAAGCATCTTATA+AGG - chr6_4:81026927-81026946 None:intergenic 25.0%
! ATGTAATTCAAACTTCTCTT+AGG + chr6_4:81027036-81027055 Msa0999230:intron 25.0%
! TGTAATTCAAACTTCTCTTA+GGG + chr6_4:81027037-81027056 Msa0999230:intron 25.0%
! AAACTCTTATCTTTCTTATG+CGG + chr6_4:81027387-81027406 Msa0999230:intron 25.0%
!! GGAAAGGAAATATTTGAAAT+TGG - chr6_4:81027878-81027897 None:intergenic 25.0%
! CAAGTTACAAATACAACTAT+TGG + chr6_4:81028085-81028104 Msa0999230:CDS 25.0%
! AAAAGGTAAATGATTACCTA+GGG - chr6_4:81028449-81028468 None:intergenic 25.0%
! AAAAAGGTAAATGATTACCT+AGG - chr6_4:81028450-81028469 None:intergenic 25.0%
! ATATATGATTCAAGACATCA+TGG + chr6_4:81028556-81028575 Msa0999230:intron 25.0%
! ACTTCTAATTATAAAGAACG+GGG - chr6_4:81028626-81028645 None:intergenic 25.0%
! ATAAATTTATGTCACTGTCT+TGG - chr6_4:81028673-81028692 None:intergenic 25.0%
! GGTGAAAAATCGATAATTAA+GGG - chr6_4:81028901-81028920 None:intergenic 25.0%
! TGGTGAAAAATCGATAATTA+AGG - chr6_4:81028902-81028921 None:intergenic 25.0%
!!! GGATTTTTTTTTGTGATGAT+AGG - chr6_4:81029224-81029243 None:intergenic 25.0%
! TCTGAAAGTTATGAATCTTA+TGG + chr6_4:81029350-81029369 Msa0999230:CDS 25.0%
! TAATAATCCTTTCTTGAACA+TGG + chr6_4:81029394-81029413 Msa0999230:CDS 25.0%
! TTATTAACCATGTTCAAGAA+AGG - chr6_4:81029404-81029423 None:intergenic 25.0%
! ATGCCTTATGGATATATTAA+TGG + chr6_4:81029434-81029453 Msa0999230:CDS 25.0%
! TGTCCATTAATATATCCATA+AGG - chr6_4:81029440-81029459 None:intergenic 25.0%
!! ATGATTTTCTTCGACTTAAT+GGG + chr6_4:81029558-81029577 Msa0999230:CDS 25.0%
AACAACAACAACAACAACAA+GGG + chr6_4:81026330-81026349 Msa0999230:CDS 30.0%
! GTACTTGATCGATTTAAATG+AGG + chr6_4:81026407-81026426 Msa0999230:CDS 30.0%
!! ACGCACCATTATTTTTCAAT+AGG + chr6_4:81026671-81026690 Msa0999230:intron 30.0%
!!! TATTTTAACATAAGAGGGGA+GGG - chr6_4:81027114-81027133 None:intergenic 30.0%
!!! ATATTTTAACATAAGAGGGG+AGG - chr6_4:81027113-81027132 None:intergenic 30.0%
!! AATTTTACTTTTCAAGGGAG+GGG - chr6_4:81026892-81026911 None:intergenic 30.0%
GTAATTCAAACTTCTCTTAG+GGG + chr6_4:81027038-81027057 Msa0999230:intron 30.0%
!!! ATATTTTAACATAAGAGGGG+AGG + chr6_4:81027113-81027132 Msa0999230:intron 30.0%
!!! TATTTTAACATAAGAGGGGA+GGG + chr6_4:81027114-81027133 Msa0999230:intron 30.0%
TGTAATTCAAGCATCTTTGA+GGG + chr6_4:81027140-81027159 Msa0999230:intron 30.0%
GTTGTTGGTGTAATTGAAAA+AGG - chr6_4:81027469-81027488 None:intergenic 30.0%
GTAATTCGATTGAATCCATT+AGG + chr6_4:81027787-81027806 Msa0999230:intron 30.0%
TAATTGCATTACTTTGCACA+TGG - chr6_4:81027943-81027962 None:intergenic 30.0%
!! TGTAACTTGTTGTTCATCTA+AGG - chr6_4:81028074-81028093 None:intergenic 30.0%
TCCTAATTATTATGCAATGC+AGG + chr6_4:81028262-81028281 Msa0999230:intron 30.0%
TCCTGCATTGCATAATAATT+AGG - chr6_4:81028266-81028285 None:intergenic 30.0%
! TCAATTCTGAACACTACTTT+TGG - chr6_4:81028348-81028367 None:intergenic 30.0%
AATGAAGACAAAGTGAATGA+TGG + chr6_4:81028372-81028391 Msa0999230:CDS 30.0%
! TAATCATTTACCTTTTTCCC+TGG + chr6_4:81028453-81028472 Msa0999230:intron 30.0%
CCTCTTTCATATATACACTA+AGG - chr6_4:81028535-81028554 None:intergenic 30.0%
CCTTAGTGTATATATGAAAG+AGG + chr6_4:81028532-81028551 Msa0999230:intron 30.0%
CTTCTAATTATAAAGAACGG+GGG - chr6_4:81028625-81028644 None:intergenic 30.0%
! GTCACATTTGAGACAATAAA+CGG - chr6_4:81028709-81028728 None:intergenic 30.0%
!! ACGATAAATCACTTTTAGCA+GGG - chr6_4:81028824-81028843 None:intergenic 30.0%
!! TACGATAAATCACTTTTAGC+AGG - chr6_4:81028825-81028844 None:intergenic 30.0%
TATGAAGGCAAACATAATCA+CGG + chr6_4:81029194-81029213 Msa0999230:CDS 30.0%
AAGATTCATAACTTTCAGAG+CGG - chr6_4:81029349-81029368 None:intergenic 30.0%
! ATTTGAAATCCACCAATACT+GGG - chr6_4:81029515-81029534 None:intergenic 30.0%
! TATTTGAAATCCACCAATAC+TGG - chr6_4:81029516-81029535 None:intergenic 30.0%
! CATGATTTTCTTCGACTTAA+TGG + chr6_4:81029557-81029576 Msa0999230:CDS 30.0%
CAACAACAACAACAACAACA+AGG + chr6_4:81026329-81026348 Msa0999230:CDS 35.0%
GGAAACCAAGTACTAAATTC+TGG + chr6_4:81026351-81026370 Msa0999230:CDS 35.0%
CAACTCCAGAATTTAGTACT+TGG - chr6_4:81026359-81026378 None:intergenic 35.0%
!! AAATTCTGGAGTTGATTGCT+CGG + chr6_4:81026365-81026384 Msa0999230:CDS 35.0%
TAATAATCACATAAGCGAGG+TGG - chr6_4:81026636-81026655 None:intergenic 35.0%
! TGACAAACGGATTCCTAATT+AGG + chr6_4:81026748-81026767 Msa0999230:intron 35.0%
TACATGTTGGATTGAATCGA+CGG - chr6_4:81026774-81026793 None:intergenic 35.0%
!! ATTTTAACATAAGAGGGGAG+GGG - chr6_4:81027115-81027134 None:intergenic 35.0%
TTCAAGCATCTTATAAGGGA+GGG - chr6_4:81026922-81026941 None:intergenic 35.0%
ATTCAAGCATCTTATAAGGG+AGG - chr6_4:81026923-81026942 None:intergenic 35.0%
TTTATGATACATAAGAGGGG+AGG + chr6_4:81027010-81027029 Msa0999230:intron 35.0%
!! ATTTTAACATAAGAGGGGAG+GGG + chr6_4:81027115-81027134 Msa0999230:intron 35.0%
CTGTAATTCAAGCATCTTTG+AGG + chr6_4:81027139-81027158 Msa0999230:intron 35.0%
GTAATTCAAGCATCTTTGAG+GGG + chr6_4:81027141-81027160 Msa0999230:intron 35.0%
!! GGTCATATTTGTTGTGTTGT+TGG - chr6_4:81027484-81027503 None:intergenic 35.0%
TTGGATGTTGAACTTCATCT+TGG - chr6_4:81027859-81027878 None:intergenic 35.0%
! TTGATCAAGTGTTGTTGGAA+AGG - chr6_4:81027894-81027913 None:intergenic 35.0%
TTTCCAACAACACTTGATCA+AGG + chr6_4:81027893-81027912 Msa0999230:CDS 35.0%
GCTAAACGCTGAAATTCTAA+AGG + chr6_4:81027964-81027983 Msa0999230:CDS 35.0%
! GAAATTCTAAAGGCTAGGAA+AGG + chr6_4:81027974-81027993 Msa0999230:CDS 35.0%
AAGCAACATCATTCCTTCAA+TGG - chr6_4:81028125-81028144 None:intergenic 35.0%
CATAATGATACTAGTGCTAG+TGG + chr6_4:81028148-81028167 Msa0999230:CDS 35.0%
TTATTATGCAATGCAGGAAG+AGG + chr6_4:81028268-81028287 Msa0999230:intron 35.0%
CAACATTTATCATGGTGCAA+GGG - chr6_4:81028323-81028342 None:intergenic 35.0%
! TTTTGGCGCAACATTTATCA+TGG - chr6_4:81028331-81028350 None:intergenic 35.0%
AAGTGAATGATGGTTATCGT+TGG + chr6_4:81028382-81028401 Msa0999230:CDS 35.0%
AAGGAAATGCTAAATACCCT+AGG + chr6_4:81028430-81028449 Msa0999230:CDS 35.0%
!! CGATAAATCACTTTTAGCAG+GGG - chr6_4:81028823-81028842 None:intergenic 35.0%
AACCTGTAATGTGAGAAAAC+AGG + chr6_4:81029130-81029149 Msa0999230:CDS 35.0%
AGTGTAGTACTTGACTTTGT+TGG - chr6_4:81029245-81029264 None:intergenic 35.0%
CTTATGGTAAGAACTGTACT+TGG + chr6_4:81029366-81029385 Msa0999230:CDS 35.0%
! AATACCATGATGATGCCTTA+TGG + chr6_4:81029422-81029441 Msa0999230:CDS 35.0%
! ATATCCATAAGGCATCATCA+TGG - chr6_4:81029429-81029448 None:intergenic 35.0%
! AAATCATGAAGTTCTGGTAG+TGG - chr6_4:81029545-81029564 None:intergenic 35.0%
CGAAGAAAATCATGAAGTTC+TGG - chr6_4:81029551-81029570 None:intergenic 35.0%
! GCTACAATGTTTCCTTTTGA+AGG + chr6_4:81029596-81029615 Msa0999230:CDS 35.0%
!! TCGTTGGCTTCATGATTAAT+TGG - chr6_4:81029629-81029648 None:intergenic 35.0%
! TTTTTGTTGCATTGGAGCAG+AGG - chr6_4:81026473-81026492 None:intergenic 40.0%
ATTGAATCGACGGCCTAATT+AGG - chr6_4:81026764-81026783 None:intergenic 40.0%
GTAACTCAAGCTTCCTTGAA+GGG - chr6_4:81026836-81026855 None:intergenic 40.0%
TGTAACTCAAGCTTCCTTGA+AGG - chr6_4:81026837-81026856 None:intergenic 40.0%
TCAAGCATCTTATAAGGGAG+GGG - chr6_4:81026921-81026940 None:intergenic 40.0%
ATTCAAACTTCTCTTAGGGG+AGG + chr6_4:81027041-81027060 Msa0999230:intron 40.0%
TTCAAACTTCTCTTAGGGGA+GGG + chr6_4:81027042-81027061 Msa0999230:intron 40.0%
!! TGTTTTGAATAAGAGGGGAG+TGG + chr6_4:81027085-81027104 Msa0999230:intron 40.0%
ATCCATTAGGCTGTTGTTGA+TGG + chr6_4:81027800-81027819 Msa0999230:intron 40.0%
AACCATCAACAACAGCCTAA+TGG - chr6_4:81027805-81027824 None:intergenic 40.0%
!! TGTTGTTGATGGTTTCCACA+TGG + chr6_4:81027811-81027830 Msa0999230:CDS 40.0%
! TGTCCTTGATCAAGTGTTGT+TGG - chr6_4:81027899-81027918 None:intergenic 40.0%
ACAACACTTGATCAAGGACA+TGG + chr6_4:81027899-81027918 Msa0999230:CDS 40.0%
! ACGCTGAAATTCTAAAGGCT+AGG + chr6_4:81027969-81027988 Msa0999230:CDS 40.0%
GCATCTTCACGATAGTTCAT+TGG - chr6_4:81028007-81028026 None:intergenic 40.0%
ATCAGCTGCAACAACTTCTT+GGG - chr6_4:81028035-81028054 None:intergenic 40.0%
TGCAGCTGATGAAGCAAAAA+CGG + chr6_4:81028045-81028064 Msa0999230:CDS 40.0%
GTTGAAGCAAGTTCCATTGA+AGG + chr6_4:81028109-81028128 Msa0999230:CDS 40.0%
! CTAGTGGATCTTCTTCACAA+AGG + chr6_4:81028164-81028183 Msa0999230:CDS 40.0%
GCAACATTTATCATGGTGCA+AGG - chr6_4:81028324-81028343 None:intergenic 40.0%
GGTTATCGTTGGCATAAGTA+TGG + chr6_4:81028393-81028412 Msa0999230:CDS 40.0%
! ACATACTTGCATTGACACCA+GGG - chr6_4:81028473-81028492 None:intergenic 40.0%
TGCATTATAGGGGATACTAC+AGG + chr6_4:81029096-81029115 Msa0999230:intron 40.0%
! CACCTGTTTTCTCACATTAC+AGG - chr6_4:81029135-81029154 None:intergenic 40.0%
CTGTAATGTGAGAAAACAGG+TGG + chr6_4:81029133-81029152 Msa0999230:CDS 40.0%
ATGTGAGAAAACAGGTGGAA+AGG + chr6_4:81029138-81029157 Msa0999230:CDS 40.0%
TGTGAGAAAACAGGTGGAAA+GGG + chr6_4:81029139-81029158 Msa0999230:CDS 40.0%
ATTGGTGTAGCACCTTCAAA+AGG - chr6_4:81029611-81029630 None:intergenic 40.0%
TTCATTACTAGGACTGTCGT+TGG - chr6_4:81029645-81029664 None:intergenic 40.0%
! GAGTTGATTGCTCGGCTATA+AGG + chr6_4:81026373-81026392 Msa0999230:CDS 45.0%
TTGATTGCTCGGCTATAAGG+AGG + chr6_4:81026376-81026395 Msa0999230:CDS 45.0%
TGATTGCTCGGCTATAAGGA+GGG + chr6_4:81026377-81026396 Msa0999230:CDS 45.0%
TCCTTCTCCCGATTATTCTC+AGG + chr6_4:81026434-81026453 Msa0999230:CDS 45.0%
ACCTGAGAATAATCGGGAGA+AGG - chr6_4:81026438-81026457 None:intergenic 45.0%
TCAAACTTCTCTTAGGGGAG+GGG + chr6_4:81027043-81027062 Msa0999230:intron 45.0%
! ATTCAAGCATCTTTGAGGGG+AGG + chr6_4:81027144-81027163 Msa0999230:intron 45.0%
TGATGGTTTCCACATGGCAA+CGG + chr6_4:81027817-81027836 Msa0999230:CDS 45.0%
CATCAGCTGCAACAACTTCT+TGG - chr6_4:81028036-81028055 None:intergenic 45.0%
TTGGCATAAGTATGGACGCA+AGG + chr6_4:81028401-81028420 Msa0999230:CDS 45.0%
TATGGACGCAAGGAGATCAA+AGG + chr6_4:81028411-81028430 Msa0999230:CDS 45.0%
! TGCATTGACACCAGGGAAAA+AGG - chr6_4:81028466-81028485 None:intergenic 45.0%
! CACATACTTGCATTGACACC+AGG - chr6_4:81028474-81028493 None:intergenic 45.0%
CACTGACAACAGTAACACGT+AGG + chr6_4:81029261-81029280 Msa0999230:CDS 45.0%
GTCATAGGCAATGCAAAAGG+TGG - chr6_4:81029305-81029324 None:intergenic 45.0%
TGGGTCATAGGCAATGCAAA+AGG - chr6_4:81029308-81029327 None:intergenic 45.0%
TCATAACTTTCAGAGCGGTG+AGG - chr6_4:81029344-81029363 None:intergenic 45.0%
AATACTGGGAACAGCTTGAG+AGG - chr6_4:81029501-81029520 None:intergenic 45.0%
TCTCAAGCTGTTCCCAGTAT+TGG + chr6_4:81029500-81029519 Msa0999230:CDS 45.0%
!! TCATGAAGTTCTGGTAGTGG+TGG - chr6_4:81029542-81029561 None:intergenic 45.0%
! GTTGTTGTTGTCGTCGTCCA+TGG - chr6_4:81026319-81026338 None:intergenic 50.0%
ATTAGACTCCCCTCCCTTCA+AGG + chr6_4:81026820-81026839 Msa0999230:intron 50.0%
TCAAGCTTCCTTGAAGGGAG+GGG - chr6_4:81026831-81026850 None:intergenic 50.0%
CTCAAGCTTCCTTGAAGGGA+GGG - chr6_4:81026832-81026851 None:intergenic 50.0%
ACTCAAGCTTCCTTGAAGGG+AGG - chr6_4:81026833-81026852 None:intergenic 50.0%
GATGGTTTCCACATGGCAAC+GGG + chr6_4:81027818-81027837 Msa0999230:CDS 50.0%
GCTGCAACAACTTCTTGGGT+AGG - chr6_4:81028031-81028050 None:intergenic 50.0%
GGTGTGAAAGGTTGGGTCAT+AGG - chr6_4:81029320-81029339 None:intergenic 50.0%
! GTGAGGAGGTGTGAAAGGTT+GGG - chr6_4:81029327-81029346 None:intergenic 50.0%
TAACTTTCAGAGCGGTGAGG+AGG - chr6_4:81029341-81029360 None:intergenic 50.0%
GCTTGAGAGGCAGCAATTGA+AGG - chr6_4:81029488-81029507 None:intergenic 50.0%
! CAAGCTGTTCCCAGTATTGG+TGG + chr6_4:81029503-81029522 Msa0999230:CDS 50.0%
TTCCACATGGCAACGGGAAG+AGG + chr6_4:81027824-81027843 Msa0999230:CDS 55.0%
! GGTGAGGAGGTGTGAAAGGT+TGG - chr6_4:81029328-81029347 None:intergenic 55.0%
CACCTCTTCCCGTTGCCATG+TGG - chr6_4:81027829-81027848 None:intergenic 60.0%
!! ACGGGGGCGTCAGTAGTTGT+TGG - chr6_4:81028609-81028628 None:intergenic 60.0%
! GAGCGGTGAGGAGGTGTGAA+AGG - chr6_4:81029332-81029351 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81026306 81029667 81026306 ID=Msa0999230;Name=Msa0999230
chr6_4 mRNA 81026306 81029667 81026306 ID=Msa0999230-mRNA-1;Parent=Msa0999230;Name=Msa0999230-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|4|0|418
chr6_4 exon 81026306 81026455 81026306 ID=Msa0999230-mRNA-1:exon:15433;Parent=Msa0999230-mRNA-1
chr6_4 exon 81027809 81028185 81027809 ID=Msa0999230-mRNA-1:exon:15434;Parent=Msa0999230-mRNA-1
chr6_4 exon 81028284 81028451 81028284 ID=Msa0999230-mRNA-1:exon:15435;Parent=Msa0999230-mRNA-1
chr6_4 exon 81029106 81029667 81029106 ID=Msa0999230-mRNA-1:exon:15436;Parent=Msa0999230-mRNA-1
chr6_4 CDS 81026306 81026455 81026306 ID=Msa0999230-mRNA-1:cds;Parent=Msa0999230-mRNA-1
chr6_4 CDS 81027809 81028185 81027809 ID=Msa0999230-mRNA-1:cds;Parent=Msa0999230-mRNA-1
chr6_4 CDS 81028284 81028451 81028284 ID=Msa0999230-mRNA-1:cds;Parent=Msa0999230-mRNA-1
chr6_4 CDS 81029106 81029667 81029106 ID=Msa0999230-mRNA-1:cds;Parent=Msa0999230-mRNA-1
Gene Sequence

>Msa0999230

ATGTCTCATACCATGGACGACGACAACAACAACAACAACAACAAGGGAAACCAAGTACTAAATTCTGGAGTTGATTGCTCGGCTATAAGGAGGGAGAAAAAGTACTTGATCGATTTAAATGAGGTTTGTCCTTCTCCCGATTATTCTCAGGCTGTTGTTGATGGTTTCCACATGGCAACGGGAAGAGGTGTAGAACTCCAAGATGAAGTTCAACATCCAATTTCAAATATTTCCTTTCCAACAACACTTGATCAAGGACATGGTGCAGAAAAAGAACAAGTCCATGTGCAAAGTAATGCAATTACGCTAAACGCTGAAATTCTAAAGGCTAGGAAAGGAAAGATGCCAATGAACTATCGTGAAGATGCACCTACCCAAGAAGTTGTTGCAGCTGATGAAGCAAAAACGGTATCCTTAGATGAACAACAAGTTACAAATACAACTATTGGTGTTGAAGCAAGTTCCATTGAAGGAATGATGTTGCTTCAACATAATGATACTAGTGCTAGTGGATCTTCTTCACAAAGGAAGAGGAAAACTGACTTCTCTGAACACTATAAACTCCCTTGCACCATGATAAATGTTGCGCCAAAAGTAGTGTTCAGAATTGAAATCAATGAAGACAAAGTGAATGATGGTTATCGTTGGCATAAGTATGGACGCAAGGAGATCAAAGGAAATGCTAAATACCCTAGGGGATACTACAGGTGTACAACAAAAACCTGTAATGTGAGAAAACAGGTGGAAAGGGATGCACAGAATCCGAAATATGTAATTGTTTCATATGAAGGCAAACATAATCACGGATTCCCTATCATCACAAAAAAAAATCCAACAAAGTCAAGTACTACACTGACAACAGTAACACGTAGGAATGCTAGCATTGTATCGCCACCTTTTGCATTGCCTATGACCCAACCTTTCACACCTCCTCACCGCTCTGAAAGTTATGAATCTTATGGTAAGAACTGTACTTGGCCTAATAATAATCCTTTCTTGAACATGGTTAATAATACCATGATGATGCCTTATGGATATATTAATGGACAACATTTTCGAGATTGTTATGCTGCTCCTTCAATTGCTGCCTCTCAAGCTGTTCCCAGTATTGGTGGATTTCAAATATCTCCACCACTACCAGAACTTCATGATTTTCTTCGACTTAATGGGTACAATATTGCTGCTGCTACAATGTTTCCTTTTGAAGGTGCTACACCAATTAATCATGAAGCCAACGACAGTCCTAGTAATGAATGA
Protein sequence

>Msa0999230

MSHTMDDDNNNNNNKGNQVLNSGVDCSAIRREKKYLIDLNEVCPSPDYSQAVVDGFHMATGRGVELQDEVQHPISNISFPTTLDQGHGAEKEQVHVQSNAITLNAEILKARKGKMPMNYREDAPTQEVVAADEAKTVSLDEQQVTNTTIGVEASSIEGMMLLQHNDTSASGSSSQRKRKTDFSEHYKLPCTMINVAPKVVFRIEINEDKVNDGYRWHKYGRKEIKGNAKYPRGYYRCTTKTCNVRKQVERDAQNPKYVIVSYEGKHNHGFPIITKKNPTKSSTTLTTVTRRNASIVSPPFALPMTQPFTPPHRSESYESYGKNCTWPNNNPFLNMVNNTMMMPYGYINGQHFRDCYAAPSIAASQAVPSIGGFQISPPLPELHDFLRLNGYNIAAATMFPFEGATPINHEANDSPSNE*