AlfalfaGEDB | Msa0999570

M. sativa cultivar zhongmu-4 / Msa0999570

KEGG: K04730|K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999570	XP_003620684.1	70.508	512	122	7	58	555	147	643	0.0	672

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999570	sp\|Q9LV48\|PERK1_ARATH	71.545	369	78	4	192	544	264	621	0.0	543

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999570	G7KPX0	70.508	512	122	7	58	555	147	643	0.0	672

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999570	MtrunA17_Chr6g0485761	70.508	512	122	7	58	555	147	643	0.0	672
Msa0999570	MtrunA17_Chr6g0485721	69.591	342	86	4	193	529	83	411	8.10e-164	472
Msa0999570	MtrunA17_Chr5g0403891	64.943	348	102	4	195	534	243	578	5.16e-162	474
Msa0999570	MtrunA17_Chr8g0373501	57.907	430	146	8	120	535	255	663	1.15e-161	476
Msa0999570	MtrunA17_Chr3g0144481	61.644	365	115	5	192	542	73	426	1.28e-157	458
Msa0999570	MtrunA17_Chr7g0221191	46.069	547	217	9	10	541	234	717	2.13e-154	460
Msa0999570	MtrunA17_Chr0c01g0489481	46.394	513	205	11	31	536	197	646	2.39e-139	418
Msa0999570	MtrunA17_Chr5g0413511	56.380	337	136	1	196	532	47	372	7.13e-137	402
Msa0999570	MtrunA17_Chr8g0389251	49.013	304	139	3	196	498	392	680	9.74e-96	306
Msa0999570	MtrunA17_Chr8g0341881	48.571	315	145	4	196	509	393	691	3.76e-93	301

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999570	AT3G24550.1	71.545	369	78	4	192	544	264	621	0.0	543
Msa0999570	AT3G24540.1	70.588	323	84	1	193	515	168	479	8.67e-168	485
Msa0999570	AT2G18470.1	66.289	353	98	3	192	535	268	608	1.32e-167	490
Msa0999570	AT2G18470.3	66.289	353	98	3	192	535	268	608	1.32e-167	490
Msa0999570	AT2G18470.2	66.289	353	98	3	192	535	268	608	1.32e-167	490
Msa0999570	AT4G34440.2	48.116	584	208	12	17	542	102	648	1.92e-164	483
Msa0999570	AT4G34440.1	48.116	584	208	12	17	542	102	648	1.92e-164	483
Msa0999570	AT1G52290.1	61.351	370	117	5	188	542	123	481	1.99e-164	478
Msa0999570	AT1G49270.1	50.725	552	219	9	32	546	135	670	1.22e-153	456
Msa0999570	AT5G38560.1	48.263	518	195	9	34	541	207	661	2.00e-152	453

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Find 162 sgRNAs with CRISPR-Local

Find 613 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATCACCAATGGCTA+TGG	0.521233	6_4:+81756949	None:intergenic
AAAGATCAGACTTACATGAA+TGG	0.560322	6_4:-81748428	Msa0999570:CDS
AAAGATTCAGATGACTCGTA+TGG	0.587003	6_4:-81748181	Msa0999570:CDS
AAAGGAGACTGCAGTCCTGA+TGG	0.530244	6_4:+81750066	None:intergenic
AAAGTCATCTGAATCTGATT+AGG	0.276319	6_4:+81748213	None:intergenic
AAAGTGCTATAACAGGTCTC+TGG	0.374023	6_4:-81754272	Msa0999570:five_prime_UTR
AACCGCCATGGAAATCACCA+TGG	0.664122	6_4:+81747183	None:intergenic
AACTGCCACACCATCAACAA+CGG	0.616297	6_4:-81754043	Msa0999570:CDS
AAGAAGACATGAGACAGTTG+AGG	0.631142	6_4:-81747611	Msa0999570:CDS
AAGCAACCATGTAGAGTTGC+TGG	0.407403	6_4:+81756916	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATGCTATAAGCTGCAAAAT+AGG	-	chr6_4:81747471-81747490	Msa0999570:three_prime_UTR	30.0%
ACCAAAATCAACTCAAATGT+GGG	-	chr6_4:81747516-81747535	Msa0999570:exon	30.0%
AGAAACCGAACCAAATTAAT+CGG	-	chr6_4:81747634-81747653	Msa0999570:CDS	30.0%
AATGGGATAGAAAATGAACA+TGG	-	chr6_4:81748305-81748324	Msa0999570:intron	30.0%
CAAATATAGCAATATCACGT+TGG	-	chr6_4:81748584-81748603	Msa0999570:intron	30.0%
AAAAAATATCCGACCTTGTT+TGG	+	chr6_4:81748799-81748818	None:intergenic	30.0%
AAGCTATTATGTAGAACTTG+TGG	+	chr6_4:81749018-81749037	None:intergenic	30.0%
CACCAAAATCAACTCAAATT+TGG	-	chr6_4:81749143-81749162	Msa0999570:CDS	30.0%
TATAAAACGCTATTGTTGGA+GGG	+	chr6_4:81749762-81749781	None:intergenic	30.0%
TTATAAAACGCTATTGTTGG+AGG	+	chr6_4:81749763-81749782	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	81747116	81757052	81747116	ID=Msa0999570;Name=Msa0999570
chr6_4	mRNA	81747116	81757052	81747116	ID=Msa0999570-mRNA-1;Parent=Msa0999570;Name=Msa0999570-mRNA-1;_AED=0.03;_eAED=0.03;_QI=306\|1\|1\|1\|0.87\|0.77\|9\|402\|556
chr6_4	exon	81747116	81747727	81747116	ID=Msa0999570-mRNA-1:exon:15618;Parent=Msa0999570-mRNA-1
chr6_4	exon	81748122	81748316	81748122	ID=Msa0999570-mRNA-1:exon:15617;Parent=Msa0999570-mRNA-1
chr6_4	exon	81748412	81748559	81748412	ID=Msa0999570-mRNA-1:exon:15616;Parent=Msa0999570-mRNA-1
chr6_4	exon	81748720	81748796	81748720	ID=Msa0999570-mRNA-1:exon:15615;Parent=Msa0999570-mRNA-1
chr6_4	exon	81749125	81749195	81749125	ID=Msa0999570-mRNA-1:exon:15614;Parent=Msa0999570-mRNA-1
chr6_4	exon	81749285	81749371	81749285	ID=Msa0999570-mRNA-1:exon:15613;Parent=Msa0999570-mRNA-1
chr6_4	exon	81749632	81750180	81749632	ID=Msa0999570-mRNA-1:exon:15612;Parent=Msa0999570-mRNA-1
chr6_4	exon	81753845	81754304	81753845	ID=Msa0999570-mRNA-1:exon:15611;Parent=Msa0999570-mRNA-1
chr6_4	exon	81756873	81757052	81756873	ID=Msa0999570-mRNA-1:exon:15610;Parent=Msa0999570-mRNA-1
chr6_4	five_prime_UTR	81756873	81757052	81756873	ID=Msa0999570-mRNA-1:five_prime_utr;Parent=Msa0999570-mRNA-1
chr6_4	five_prime_UTR	81754179	81754304	81754179	ID=Msa0999570-mRNA-1:five_prime_utr;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81753845	81754178	81753845	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81749632	81750180	81749632	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81749285	81749371	81749285	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81749125	81749195	81749125	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81748720	81748796	81748720	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81748412	81748559	81748412	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81748122	81748316	81748122	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	CDS	81747518	81747727	81747518	ID=Msa0999570-mRNA-1:cds;Parent=Msa0999570-mRNA-1
chr6_4	three_prime_UTR	81747116	81747517	81747116	ID=Msa0999570-mRNA-1:three_prime_utr;Parent=Msa0999570-mRNA-1

Gene Sequence

>Msa0999570

ATGTCGAATGCTTCGGCGGGAAGCCCAACAGCTGCACCATATTCATATTCACCACCGTCACCGCCAACAACTCCCGCAACATCACTGCCGCTTTCACCGCCATCACCATCACCAACTGCCACACCATCAACAACGGCAAACCCCAAAGCACCACCTTCATCATCATCAAATATGTCAACCGGAGCTATCGTTGGTATCGCCTTTGGAGCAGTGTTGGTTTTTGTTATGTGGATCATTTGTAGCATATGGTGTTGGAAGAAGTGGAAGAAAAACCGTGATCGAGAAAGCTTTAGTCGGAAGCAACCTCAGGAACCACAACTTCAACGAAGTCAAGTTGATCCTTATGGTGGTTCACCGCAACAATGGCAAGGACTGCAGTCTCCATCCGATCATGTGGTCTCAAAACCACTGCTTCTTGCTCAAATTCCATCAGGACTGCAGTCTCCTTTAGCACCACCATCATCGCTGTCCCATGCTTCAATTCCATCAGCACCTCTGTCTTTCGTAAGCAGCAGCAGTGGTGGCTCCGGATCAAAACATTCAGGTGTCGAACTGCTTCGATCTTCTTCTCGAAGCAAGAGCACGTTTACATATGAGGAGTTGGTGCGCGCAACAGATGGCTTCTCCGATGCTAACCTCCTAGGACAAGGGGGGTTTGGATTTGTGCACAAAGGAATTCTCCCTAATGATAAAGAGGTTGCTGTTAAGCAGCTGAAAGCTGGTAGTGGACAAGGAGAACGTGAATTTCAAGCTGAAGTTGAGATTATTAGCCGTGTTCATCACAGACATCTTGTTTCTCTTGTTGGATACTGCAGCACTGGTTTTCAGAGGCTACTTGCTTATGAATTTGTTCCTAACAATACATTGGAATTCCATTTACATGGAAAAGCACTACCGATACTTGATTGGTCAGCAAGACAGCTAATTGCTTTAGGGTCTGCGAAAGGACTTGCATATCTTCATGAAGATTGTCATCCTAAGATCATTCACCGTGATATTAAAGCTGCTAACATCCTTCTTGATTTTAAGTTTGAAGCAAAGGTTGCAGATTTTGGCCTTGCAAAGATTGCTTCTGACCTCAATACTCATGTTTCTACTCGAGTGATGGGGACTTTCGGGTATTTGGCTCCAGAATATGCTGCCAGTGGAAAACTTACAGAAAAATCAGATGTTTTCTCTTATGGAGTCGTGCTCCTTGAGTTGTTAACTGGACGACGACCTATTGATAAAGATCAGACTTACATGAATGGTAGTTTGGTTGAGTGGGCCAGGCCTTTGCTCATGCGTGCTATAGAAGAAGATAATGTAGATTCTCTGATTGATCCAAGGCTTCAGAATGAATTTGACCCTAATCAGATTCAGATGACTTTCAAACAAAAAAAGATTCAGATGACTCGTATGGTGGCATGCGCTGCAGCTTGCACGCGTCACTCAGCTATGCATCGACCAAAGATGAGTCAGGTTGTGCGAGTTCTAGAAGGAGATGTGTCACTAGCAGATCTTAATGCAGGGGTTATACCTGGACACAGCAGTGTCTACAATACTCAACAACACAAAGAAGACATGAGACAGTTGAGGAAAACAGCATTCGGAACTCAAGAGTATACTGGAATCAGCGACTACGGATCATTCAGGGAAAACATCAAGGGAAATGGAAATGAGAACAGTTAA

Protein sequence

>Msa0999570

MSNASAGSPTAAPYSYSPPSPPTTPATSLPLSPPSPSPTATPSTTANPKAPPSSSSNMSTGAIVGIAFGAVLVFVMWIICSIWCWKKWKKNRDRESFSRKQPQEPQLQRSQVDPYGGSPQQWQGLQSPSDHVVSKPLLLAQIPSGLQSPLAPPSSLSHASIPSAPLSFVSSSSGGSGSKHSGVELLRSSSRSKSTFTYEELVRATDGFSDANLLGQGGFGFVHKGILPNDKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCSTGFQRLLAYEFVPNNTLEFHLHGKALPILDWSARQLIALGSAKGLAYLHEDCHPKIIHRDIKAANILLDFKFEAKVADFGLAKIASDLNTHVSTRVMGTFGYLAPEYAASGKLTEKSDVFSYGVVLLELLTGRRPIDKDQTYMNGSLVEWARPLLMRAIEEDNVDSLIDPRLQNEFDPNQIQMTFKQKKIQMTRMVACAAACTRHSAMHRPKMSQVVRVLEGDVSLADLNAGVIPGHSSVYNTQQHKEDMRQLRKTAFGTQEYTGISDYGSFRENIKGNGNENS*