AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1001510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1001510 MtrunA17_Chr6g0488471 96.552 290 9 1 1 290 1 289 0.0 570
Msa1001510 MtrunA17_Chr8g0389241 50.325 308 107 11 1 290 1 280 2.76e-73 226
Msa1001510 MtrunA17_Chr2g0331031 52.717 184 55 5 1 162 1 174 7.01e-49 166
Msa1001510 MtrunA17_Chr4g0055931 48.634 183 72 4 1 173 1 171 1.81e-47 161
Msa1001510 MtrunA17_Chr5g0415191 46.237 186 77 5 1 175 3 176 1.20e-41 145
Msa1001510 MtrunA17_Chr3g0135671 44.751 181 80 5 1 174 3 170 1.83e-36 130
Msa1001510 MtrunA17_Chr1g0162081 60.784 102 33 2 91 185 116 217 3.47e-34 125
Msa1001510 MtrunA17_Chr4g0062741 73.239 71 19 0 91 161 132 202 6.01e-34 125
Msa1001510 MtrunA17_Chr7g0241761 78.873 71 15 0 91 161 134 204 2.09e-33 124
Msa1001510 MtrunA17_Chr4g0059701 48.780 164 69 5 32 187 15 171 8.20e-32 119
Msa1001510 MtrunA17_Chr3g0104071 73.611 72 19 0 91 162 141 212 1.37e-31 119
Msa1001510 MtrunA17_Chr5g0429521 74.648 71 18 0 91 161 126 196 2.15e-31 119
Msa1001510 MtrunA17_Chr1g0210471 63.529 85 24 1 92 176 119 196 2.12e-30 114
Msa1001510 MtrunA17_Chr5g0436501 69.863 73 20 1 91 161 119 191 3.63e-29 112
Msa1001510 MtrunA17_Chr3g0092621 60.976 82 30 1 82 161 67 148 1.19e-28 108
Msa1001510 MtrunA17_Chr6g0452791 67.797 59 19 0 97 155 80 138 1.48e-22 92.0
Msa1001510 MtrunA17_Chr5g0409841 52.857 70 31 1 93 160 88 157 2.53e-19 84.7
Msa1001510 MtrunA17_Chr5g0440731 50.704 71 32 1 93 160 101 171 6.02e-18 82.0
Msa1001510 MtrunA17_Chr5g0440741 55.385 65 26 1 99 160 102 166 1.39e-17 80.9
Msa1001510 MtrunA17_Chr5g0409831 58.929 56 23 0 93 148 73 128 9.56e-17 77.4
Msa1001510 MtrunA17_Chr8g0365011 51.948 77 23 2 99 161 94 170 3.10e-15 74.3
Msa1001510 MtrunA17_Chr1g0192061 50.633 79 33 3 99 174 63 138 4.05e-15 72.0
Msa1001510 MtrunA17_Chr8g0364731 64.912 57 18 1 107 161 11 67 4.26e-15 72.0
Msa1001510 MtrunA17_Chr5g0432921 56.250 64 24 3 99 159 115 177 2.05e-12 65.5
Msa1001510 MtrunA17_Chr5g0432941 54.688 64 25 3 99 159 115 177 6.72e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1001510 AT5G56840.1 56.345 197 53 6 1 185 1 176 1.16e-61 195
Msa1001510 AT5G47390.1 52.907 172 60 4 1 162 1 161 1.78e-49 168
Msa1001510 AT5G61620.1 49.123 171 71 6 1 161 8 172 4.64e-46 157
Msa1001510 AT3G16350.1 40.850 306 116 11 1 252 1 295 5.99e-44 154
Msa1001510 AT1G70000.1 50.857 175 61 7 1 162 1 163 8.25e-43 147
Msa1001510 AT1G70000.2 50.857 175 61 7 1 162 1 163 8.25e-43 147
Msa1001510 AT1G49010.1 66.667 81 27 0 91 171 128 208 1.43e-33 125
Msa1001510 AT5G08520.1 67.949 78 25 0 91 168 112 189 4.86e-33 123
Msa1001510 AT2G38090.1 71.250 80 21 1 85 162 127 206 1.08e-32 122
Msa1001510 AT5G58900.1 74.286 70 18 0 92 161 136 205 6.42e-31 117
Msa1001510 AT5G01200.1 70.423 71 21 0 91 161 140 210 7.33e-30 114
Msa1001510 AT3G11280.2 69.014 71 22 0 91 161 121 191 1.19e-28 110
Msa1001510 AT3G11280.1 69.014 71 22 0 91 161 121 191 1.19e-28 110
Msa1001510 AT5G04760.1 65.714 70 24 0 92 161 94 163 2.96e-28 108
Msa1001510 AT5G05790.2 67.606 71 23 0 91 161 125 195 9.71e-28 108
Msa1001510 AT5G05790.1 67.606 71 23 0 91 161 125 195 9.71e-28 108
Msa1001510 AT1G74840.2 46.259 147 68 4 23 161 17 160 8.98e-27 105
Msa1001510 AT1G74840.1 46.259 147 68 4 23 161 17 160 1.94e-26 105
Msa1001510 AT1G19000.2 65.000 80 28 0 93 172 98 177 2.33e-26 105
Msa1001510 AT1G19000.1 65.000 80 28 0 93 172 98 177 2.33e-26 105
Msa1001510 AT5G23650.1 60.563 71 28 0 91 161 114 184 2.49e-23 98.2
Msa1001510 AT3G10580.2 58.228 79 31 1 93 171 91 167 6.75e-23 95.1
Msa1001510 AT3G10580.1 58.228 79 31 1 93 171 91 167 8.98e-23 95.5
Msa1001510 AT4G09450.1 57.971 69 29 0 93 161 87 155 8.28e-20 85.5
Msa1001510 AT3G10590.1 51.316 76 34 2 89 162 101 175 7.85e-16 75.1

Find 58 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACACCAACACTATTTAAT+TGG 0.185566 6_4:-85310955 None:intergenic
GCTAATTAAGTCACACTAAT+TGG 0.200852 6_4:-85313015 None:intergenic
CATCAAGTTCATTTCTTATT+GGG 0.230277 6_4:+85312992 Msa1001510:CDS
GATGCTACATTTGAAGAATT+TGG 0.240336 6_4:-85312907 None:intergenic
TCATCAAGTTCATTTCTTAT+TGG 0.242280 6_4:+85312991 Msa1001510:CDS
AATTACAAGTCCTTGAATTA+TGG 0.245021 6_4:-85311183 None:intergenic
TTGTTCCATGTTATTTGAAT+TGG 0.302002 6_4:-85312959 None:intergenic
CATATCATAATCATCTGATT+TGG 0.325159 6_4:-85312788 None:intergenic
TTGTGTTTATTGTCTCTTAG+AGG 0.354122 6_4:-85311078 None:intergenic
GCGGTGGAATTTGTGAAAAT+TGG 0.355606 6_4:-85310980 None:intergenic
ATATTTAAAGACTTTGTTAG+AGG 0.361817 6_4:-85312871 None:intergenic
ACGTCTCTTCTTCTTATTCA+AGG 0.365533 6_4:-85311835 None:intergenic
CTATGCTCTTCTTCTGTCCA+TGG 0.368105 6_4:-85311684 None:intergenic
ACCAACACTATTTAATTGGT+TGG 0.373969 6_4:-85310951 None:intergenic
AGGACTTGTAATTCATTAAG+AGG 0.396462 6_4:+85311193 Msa1001510:CDS
AGAGACAATAAACACAAGTT+TGG 0.398819 6_4:+85311084 Msa1001510:five_prime_UTR
GAGCATAGAAAATTCCTTGT+TGG 0.413219 6_4:+85311699 Msa1001510:CDS
CTTGTTGGACTTGAGAAGCT+TGG 0.417301 6_4:+85311714 Msa1001510:CDS
GTTAGAGGATGTAGCCAATC+TGG 0.418818 6_4:-85312856 None:intergenic
TCTTCATAAATTACATACAT+AGG 0.424154 6_4:-85313070 None:intergenic
ATTGATGATAGAGCCTCTAT+TGG 0.438397 6_4:+85311364 Msa1001510:CDS
CACAAAAGAACTTATGTGTC+TGG 0.441747 6_4:-85311031 None:intergenic
GAAGAAGAAAAGGAAGAAGT+AGG 0.461242 6_4:-85311331 None:intergenic
TCTATTGGTTATCTTTCAGA+TGG 0.468296 6_4:+85311379 Msa1001510:CDS
CTTCATAAATTACATACATA+GGG 0.469802 6_4:-85313069 None:intergenic
GGACTTGAGAAGCTTGGAAA+AGG 0.471359 6_4:+85311720 Msa1001510:CDS
GGATAATCTTGAAGAAGAAA+AGG 0.479396 6_4:-85311341 None:intergenic
GTGATATATATGATGATTGA+TGG 0.479509 6_4:-85311009 None:intergenic
GATCTTGAGCTAACTCTGGC+AGG 0.479985 6_4:+85312934 Msa1001510:CDS
AATAGAGGCTCTATCATCAA+TGG 0.491413 6_4:-85311362 None:intergenic
GAAACATAGGCCATAATTCA+AGG 0.492974 6_4:+85311173 Msa1001510:CDS
AGAAGCTTGGAAAAGGAGAC+TGG 0.494349 6_4:+85311727 Msa1001510:CDS
TTTATTCATTAGGAGTTCCA+TGG 0.499440 6_4:+85311667 Msa1001510:intron
ATCTGAAAGATAACCAATAG+AGG 0.500436 6_4:-85311377 None:intergenic
GCCAGAGTTAGCTCAAGATC+AGG 0.513405 6_4:-85312931 None:intergenic
TCATAGTTCGTACACAAGAA+AGG 0.523916 6_4:+85311404 Msa1001510:CDS
TGTGTTTATTGTCTCTTAGA+GGG 0.525698 6_4:-85311077 None:intergenic
GCATGGCTTGCAACTTGTGT+TGG 0.527834 6_4:-85311786 None:intergenic
GTAGGAGAAGAAGATGAAGT+AGG 0.532464 6_4:-85311313 None:intergenic
TCCAACCAATTAAATAGTGT+TGG 0.537821 6_4:+85310950 Msa1001510:five_prime_UTR
AAAGCAAAGAAATAAATAAG+TGG 0.539050 6_4:+85313183 Msa1001510:three_prime_UTR
TTTAAGGTTAAGGTTTGTGT+CGG 0.548532 6_4:+85311117 Msa1001510:five_prime_UTR
AAAGTCTTTAAATATGACAT+TGG 0.555274 6_4:+85312879 Msa1001510:CDS
TGGACATAGTTTATTCAATG+TGG 0.555850 6_4:-85310931 None:intergenic
TGCTCACATTGTGGAAACAT+AGG 0.564462 6_4:+85311160 Msa1001510:CDS
GCTTGGAAAAGGAGACTGGA+GGG 0.565371 6_4:+85311731 Msa1001510:CDS
ACCTGATCTTGAGCTAACTC+TGG 0.566325 6_4:+85312930 Msa1001510:CDS
ATCTTGAGCTAACTCTGGCA+GGG 0.569532 6_4:+85312935 Msa1001510:CDS
GGCAGAAAGTGCTCACATTG+TGG 0.586228 6_4:+85311151 Msa1001510:CDS
CAAGCTTCTCAAGTCCAACA+AGG 0.608243 6_4:-85311713 None:intergenic
TGATGATCATGAAGTTGAGA+AGG 0.617743 6_4:+85312723 Msa1001510:CDS
TGATATATATGATGATTGAT+GGG 0.621044 6_4:-85311008 None:intergenic
TGATTGATGGGTGTGAGCGG+TGG 0.632800 6_4:-85310996 None:intergenic
ACGTTCAAGTCTCTTTGACA+TGG 0.636224 6_4:+85311854 Msa1001510:CDS
AGCTTGGAAAAGGAGACTGG+AGG 0.640674 6_4:+85311730 Msa1001510:CDS
AGGGTCCAATTCAAATAACA+TGG 0.651228 6_4:+85312954 Msa1001510:CDS
CTTGGAAAAGGAGACTGGAG+GGG 0.658703 6_4:+85311732 Msa1001510:CDS
TGATGATTGATGGGTGTGAG+CGG 0.665275 6_4:-85310999 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAACTTTTACAAAAT+GGG - chr6_4:85312073-85312092 None:intergenic 10.0%
!!! TAAAAAAAACTTTTACAAAA+TGG - chr6_4:85312074-85312093 None:intergenic 10.0%
!!! TTAATGTTATTTTAGAATTT+GGG - chr6_4:85312279-85312298 None:intergenic 10.0%
!!! TTTAATGTTATTTTAGAATT+TGG - chr6_4:85312280-85312299 None:intergenic 10.0%
!!! AAAAAAAACTTTTACAAAAT+GGG - chr6_4:85312073-85312092 None:intergenic 10.0%
!!! TAAAAAAAACTTTTACAAAA+TGG - chr6_4:85312074-85312093 None:intergenic 10.0%
!!! TTAATGTTATTTTAGAATTT+GGG - chr6_4:85312279-85312298 None:intergenic 10.0%
!!! TTTAATGTTATTTTAGAATT+TGG - chr6_4:85312280-85312299 None:intergenic 10.0%
!!! ATCTTGTTATTTTATTCATT+AGG + chr6_4:85311657-85311676 Msa1001510:intron 15.0%
!! ATTATAAAAAGAAGATTCTT+CGG - chr6_4:85312239-85312258 None:intergenic 15.0%
!!! CTTTTTATAATTGTTGAATT+AGG + chr6_4:85312248-85312267 Msa1001510:intron 15.0%
!!! ATCTTGTTATTTTATTCATT+AGG + chr6_4:85311657-85311676 Msa1001510:intron 15.0%
!! ATTATAAAAAGAAGATTCTT+CGG - chr6_4:85312239-85312258 None:intergenic 15.0%
!!! CTTTTTATAATTGTTGAATT+AGG + chr6_4:85312248-85312267 Msa1001510:intron 15.0%
!! TGATATATATGATGATTGAT+GGG - chr6_4:85311011-85311030 None:intergenic 20.0%
!!! TATTCTAGTTTTTAAGGTTA+AGG + chr6_4:85311107-85311126 Msa1001510:five_prime_UTR 20.0%
!! TTAACAAAAGCAATAACAAA+GGG - chr6_4:85311492-85311511 None:intergenic 20.0%
!! ATTAACAAAAGCAATAACAA+AGG - chr6_4:85311493-85311512 None:intergenic 20.0%
!! CTATGATGTATTTATTATGA+AGG + chr6_4:85311519-85311538 Msa1001510:intron 20.0%
!! GTATGCATTTATTGAATTTA+AGG - chr6_4:85312012-85312031 None:intergenic 20.0%
!!! GAAAAAACACATTTTTTTTC+CGG - chr6_4:85312166-85312185 None:intergenic 20.0%
!! TTAGTTAAATAGTCCTAATA+AGG - chr6_4:85312492-85312511 None:intergenic 20.0%
!!! TGATATTGTGGAAATTTTTT+AGG + chr6_4:85312621-85312640 Msa1001510:intron 20.0%
!! ATATTTAAAGACTTTGTTAG+AGG - chr6_4:85312874-85312893 None:intergenic 20.0%
!! AAAGTCTTTAAATATGACAT+TGG + chr6_4:85312879-85312898 Msa1001510:CDS 20.0%
!! CTTCATAAATTACATACATA+GGG - chr6_4:85313072-85313091 None:intergenic 20.0%
!! TCTTCATAAATTACATACAT+AGG - chr6_4:85313073-85313092 None:intergenic 20.0%
!! AAAGCAAAGAAATAAATAAG+TGG + chr6_4:85313183-85313202 Msa1001510:three_prime_UTR 20.0%
!! TGATATATATGATGATTGAT+GGG - chr6_4:85311011-85311030 None:intergenic 20.0%
!!! TATTCTAGTTTTTAAGGTTA+AGG + chr6_4:85311107-85311126 Msa1001510:five_prime_UTR 20.0%
!! TTAACAAAAGCAATAACAAA+GGG - chr6_4:85311492-85311511 None:intergenic 20.0%
!! ATTAACAAAAGCAATAACAA+AGG - chr6_4:85311493-85311512 None:intergenic 20.0%
!! CTATGATGTATTTATTATGA+AGG + chr6_4:85311519-85311538 Msa1001510:intron 20.0%
!! GTATGCATTTATTGAATTTA+AGG - chr6_4:85312012-85312031 None:intergenic 20.0%
!!! GAAAAAACACATTTTTTTTC+CGG - chr6_4:85312166-85312185 None:intergenic 20.0%
!! TTAGTTAAATAGTCCTAATA+AGG - chr6_4:85312492-85312511 None:intergenic 20.0%
!!! TGATATTGTGGAAATTTTTT+AGG + chr6_4:85312621-85312640 Msa1001510:intron 20.0%
!! ATATTTAAAGACTTTGTTAG+AGG - chr6_4:85312874-85312893 None:intergenic 20.0%
!! AAAGTCTTTAAATATGACAT+TGG + chr6_4:85312879-85312898 Msa1001510:CDS 20.0%
!! CTTCATAAATTACATACATA+GGG - chr6_4:85313072-85313091 None:intergenic 20.0%
!! TCTTCATAAATTACATACAT+AGG - chr6_4:85313073-85313092 None:intergenic 20.0%
!! AAAGCAAAGAAATAAATAAG+TGG + chr6_4:85313183-85313202 Msa1001510:three_prime_UTR 20.0%
! GTGATATATATGATGATTGA+TGG - chr6_4:85311012-85311031 None:intergenic 25.0%
!!! GTTTGGTATTCTAGTTTTTA+AGG + chr6_4:85311101-85311120 Msa1001510:five_prime_UTR 25.0%
! AATTACAAGTCCTTGAATTA+TGG - chr6_4:85311186-85311205 None:intergenic 25.0%
!! GAATAAAATACTTTTGAGCA+TGG - chr6_4:85311806-85311825 None:intergenic 25.0%
!! TTATTGAATTTAAGGTCATG+AGG - chr6_4:85312004-85312023 None:intergenic 25.0%
! TCTGAATTTAGTCATTTACA+CGG - chr6_4:85312188-85312207 None:intergenic 25.0%
!!! TGAATGGTTTTTAAGAATCA+TGG + chr6_4:85312210-85312229 Msa1001510:intron 25.0%
! ATAGTCAATCTTAACCATAA+GGG + chr6_4:85312353-85312372 Msa1001510:intron 25.0%
! TTAGAGACTTATTTGTTCTA+TGG + chr6_4:85312432-85312451 Msa1001510:intron 25.0%
! CAATGAAATTAGTCCTTATT+AGG + chr6_4:85312476-85312495 Msa1001510:intron 25.0%
! TCTTGAATTGAATGATATTG+TGG + chr6_4:85312609-85312628 Msa1001510:intron 25.0%
!!! ATTGTGGAAATTTTTTAGGT+TGG + chr6_4:85312625-85312644 Msa1001510:intron 25.0%
! CATATCATAATCATCTGATT+TGG - chr6_4:85312791-85312810 None:intergenic 25.0%
!! TTGTTCCATGTTATTTGAAT+TGG - chr6_4:85312962-85312981 None:intergenic 25.0%
! TCATCAAGTTCATTTCTTAT+TGG + chr6_4:85312991-85313010 Msa1001510:CDS 25.0%
! CATCAAGTTCATTTCTTATT+GGG + chr6_4:85312992-85313011 Msa1001510:CDS 25.0%
! GTGATATATATGATGATTGA+TGG - chr6_4:85311012-85311031 None:intergenic 25.0%
!!! GTTTGGTATTCTAGTTTTTA+AGG + chr6_4:85311101-85311120 Msa1001510:five_prime_UTR 25.0%
! AATTACAAGTCCTTGAATTA+TGG - chr6_4:85311186-85311205 None:intergenic 25.0%
!! GAATAAAATACTTTTGAGCA+TGG - chr6_4:85311806-85311825 None:intergenic 25.0%
!! TTATTGAATTTAAGGTCATG+AGG - chr6_4:85312004-85312023 None:intergenic 25.0%
! TCTGAATTTAGTCATTTACA+CGG - chr6_4:85312188-85312207 None:intergenic 25.0%
!!! TGAATGGTTTTTAAGAATCA+TGG + chr6_4:85312210-85312229 Msa1001510:intron 25.0%
! ATAGTCAATCTTAACCATAA+GGG + chr6_4:85312353-85312372 Msa1001510:intron 25.0%
! TTAGAGACTTATTTGTTCTA+TGG + chr6_4:85312432-85312451 Msa1001510:intron 25.0%
! CAATGAAATTAGTCCTTATT+AGG + chr6_4:85312476-85312495 Msa1001510:intron 25.0%
! TCTTGAATTGAATGATATTG+TGG + chr6_4:85312609-85312628 Msa1001510:intron 25.0%
!!! ATTGTGGAAATTTTTTAGGT+TGG + chr6_4:85312625-85312644 Msa1001510:intron 25.0%
! CATATCATAATCATCTGATT+TGG - chr6_4:85312791-85312810 None:intergenic 25.0%
!! TTGTTCCATGTTATTTGAAT+TGG - chr6_4:85312962-85312981 None:intergenic 25.0%
! TCATCAAGTTCATTTCTTAT+TGG + chr6_4:85312991-85313010 Msa1001510:CDS 25.0%
! CATCAAGTTCATTTCTTATT+GGG + chr6_4:85312992-85313011 Msa1001510:CDS 25.0%
TGGACATAGTTTATTCAATG+TGG - chr6_4:85310934-85310953 None:intergenic 30.0%
TCCAACCAATTAAATAGTGT+TGG + chr6_4:85310950-85310969 Msa1001510:five_prime_UTR 30.0%
ACCAACACTATTTAATTGGT+TGG - chr6_4:85310954-85310973 None:intergenic 30.0%
! ACACATAAGTTCTTTTGTGT+TGG + chr6_4:85311035-85311054 Msa1001510:five_prime_UTR 30.0%
TGTGTTTATTGTCTCTTAGA+GGG - chr6_4:85311080-85311099 None:intergenic 30.0%
TTGTGTTTATTGTCTCTTAG+AGG - chr6_4:85311081-85311100 None:intergenic 30.0%
AGAGACAATAAACACAAGTT+TGG + chr6_4:85311084-85311103 Msa1001510:five_prime_UTR 30.0%
! TTTAAGGTTAAGGTTTGTGT+CGG + chr6_4:85311117-85311136 Msa1001510:five_prime_UTR 30.0%
!! TTTGTGTCGGTTCAAAAAAT+GGG + chr6_4:85311130-85311149 Msa1001510:exon 30.0%
AGGACTTGTAATTCATTAAG+AGG + chr6_4:85311193-85311212 Msa1001510:CDS 30.0%
! TTTGTTGGAGTTAGACTTTT+TGG + chr6_4:85311226-85311245 Msa1001510:CDS 30.0%
!! CATGCTAAAACTCTTTTTCA+TGG - chr6_4:85311284-85311303 None:intergenic 30.0%
!! CATGAAAAAGAGTTTTAGCA+TGG + chr6_4:85311282-85311301 Msa1001510:CDS 30.0%
GGATAATCTTGAAGAAGAAA+AGG - chr6_4:85311344-85311363 None:intergenic 30.0%
ATCTGAAAGATAACCAATAG+AGG - chr6_4:85311380-85311399 None:intergenic 30.0%
! TCTATTGGTTATCTTTCAGA+TGG + chr6_4:85311379-85311398 Msa1001510:CDS 30.0%
TTTATTCATTAGGAGTTCCA+TGG + chr6_4:85311667-85311686 Msa1001510:intron 30.0%
!! GTGCTTTTTAAGAAATCAGA+GGG - chr6_4:85312099-85312118 None:intergenic 30.0%
!! TGTGCTTTTTAAGAAATCAG+AGG - chr6_4:85312100-85312119 None:intergenic 30.0%
!! ATGGTTTTTAAGAATCATGG+TGG + chr6_4:85312213-85312232 Msa1001510:intron 30.0%
GATAGTCAATCTTAACCATA+AGG + chr6_4:85312352-85312371 Msa1001510:intron 30.0%
TAGTCAATCTTAACCATAAG+GGG + chr6_4:85312354-85312373 Msa1001510:intron 30.0%
TTGTTTCCTCTAAGCATATT+TGG - chr6_4:85312397-85312416 None:intergenic 30.0%
TATGCTTAGAGGAAACAAAT+TGG + chr6_4:85312399-85312418 Msa1001510:intron 30.0%
!!! GAAATTTTTTAGGTTGGTAG+TGG + chr6_4:85312631-85312650 Msa1001510:intron 30.0%
GATGCTACATTTGAAGAATT+TGG - chr6_4:85312910-85312929 None:intergenic 30.0%
GCTAATTAAGTCACACTAAT+TGG - chr6_4:85313018-85313037 None:intergenic 30.0%
! ACTACACTTTGAAAAATGAC+AGG - chr6_4:85313115-85313134 None:intergenic 30.0%
TGGACATAGTTTATTCAATG+TGG - chr6_4:85310934-85310953 None:intergenic 30.0%
TCCAACCAATTAAATAGTGT+TGG + chr6_4:85310950-85310969 Msa1001510:five_prime_UTR 30.0%
ACCAACACTATTTAATTGGT+TGG - chr6_4:85310954-85310973 None:intergenic 30.0%
! ACACATAAGTTCTTTTGTGT+TGG + chr6_4:85311035-85311054 Msa1001510:five_prime_UTR 30.0%
TGTGTTTATTGTCTCTTAGA+GGG - chr6_4:85311080-85311099 None:intergenic 30.0%
TTGTGTTTATTGTCTCTTAG+AGG - chr6_4:85311081-85311100 None:intergenic 30.0%
AGAGACAATAAACACAAGTT+TGG + chr6_4:85311084-85311103 Msa1001510:five_prime_UTR 30.0%
! TTTAAGGTTAAGGTTTGTGT+CGG + chr6_4:85311117-85311136 Msa1001510:five_prime_UTR 30.0%
!! TTTGTGTCGGTTCAAAAAAT+GGG + chr6_4:85311130-85311149 Msa1001510:exon 30.0%
AGGACTTGTAATTCATTAAG+AGG + chr6_4:85311193-85311212 Msa1001510:CDS 30.0%
! TTTGTTGGAGTTAGACTTTT+TGG + chr6_4:85311226-85311245 Msa1001510:CDS 30.0%
!! CATGCTAAAACTCTTTTTCA+TGG - chr6_4:85311284-85311303 None:intergenic 30.0%
!! CATGAAAAAGAGTTTTAGCA+TGG + chr6_4:85311282-85311301 Msa1001510:CDS 30.0%
GGATAATCTTGAAGAAGAAA+AGG - chr6_4:85311344-85311363 None:intergenic 30.0%
ATCTGAAAGATAACCAATAG+AGG - chr6_4:85311380-85311399 None:intergenic 30.0%
! TCTATTGGTTATCTTTCAGA+TGG + chr6_4:85311379-85311398 Msa1001510:CDS 30.0%
TTTATTCATTAGGAGTTCCA+TGG + chr6_4:85311667-85311686 Msa1001510:intron 30.0%
!! GTGCTTTTTAAGAAATCAGA+GGG - chr6_4:85312099-85312118 None:intergenic 30.0%
!! TGTGCTTTTTAAGAAATCAG+AGG - chr6_4:85312100-85312119 None:intergenic 30.0%
!! ATGGTTTTTAAGAATCATGG+TGG + chr6_4:85312213-85312232 Msa1001510:intron 30.0%
GATAGTCAATCTTAACCATA+AGG + chr6_4:85312352-85312371 Msa1001510:intron 30.0%
TAGTCAATCTTAACCATAAG+GGG + chr6_4:85312354-85312373 Msa1001510:intron 30.0%
TTGTTTCCTCTAAGCATATT+TGG - chr6_4:85312397-85312416 None:intergenic 30.0%
TATGCTTAGAGGAAACAAAT+TGG + chr6_4:85312399-85312418 Msa1001510:intron 30.0%
!!! GAAATTTTTTAGGTTGGTAG+TGG + chr6_4:85312631-85312650 Msa1001510:intron 30.0%
GATGCTACATTTGAAGAATT+TGG - chr6_4:85312910-85312929 None:intergenic 30.0%
GCTAATTAAGTCACACTAAT+TGG - chr6_4:85313018-85313037 None:intergenic 30.0%
! ACTACACTTTGAAAAATGAC+AGG - chr6_4:85313115-85313134 None:intergenic 30.0%
GCACACCAACACTATTTAAT+TGG - chr6_4:85310958-85310977 None:intergenic 35.0%
CACAAAAGAACTTATGTGTC+TGG - chr6_4:85311034-85311053 None:intergenic 35.0%
!! GTTTGTGTCGGTTCAAAAAA+TGG + chr6_4:85311129-85311148 Msa1001510:exon 35.0%
GAAACATAGGCCATAATTCA+AGG + chr6_4:85311173-85311192 Msa1001510:CDS 35.0%
!!! AGAGGAAGTAGTAGTTTTGT+TGG + chr6_4:85311211-85311230 Msa1001510:CDS 35.0%
GAAGAAGAAAAGGAAGAAGT+AGG - chr6_4:85311334-85311353 None:intergenic 35.0%
AATAGAGGCTCTATCATCAA+TGG - chr6_4:85311365-85311384 None:intergenic 35.0%
! ATTGATGATAGAGCCTCTAT+TGG + chr6_4:85311364-85311383 Msa1001510:CDS 35.0%
TCATAGTTCGTACACAAGAA+AGG + chr6_4:85311404-85311423 Msa1001510:CDS 35.0%
CGTACACAAGAAAGGAAAAA+AGG + chr6_4:85311412-85311431 Msa1001510:CDS 35.0%
GAGCATAGAAAATTCCTTGT+TGG + chr6_4:85311699-85311718 Msa1001510:CDS 35.0%
ACGTCTCTTCTTCTTATTCA+AGG - chr6_4:85311838-85311857 None:intergenic 35.0%
ACGCATTTGGATGTATAATC+CGG + chr6_4:85312144-85312163 Msa1001510:intron 35.0%
TGACTAAATTCAGACGTGAA+TGG + chr6_4:85312194-85312213 Msa1001510:intron 35.0%
TCAAGTGATCTTAGAGTGAT+CGG - chr6_4:85312321-85312340 None:intergenic 35.0%
AGAGTTCCAAATATGCTTAG+AGG + chr6_4:85312388-85312407 Msa1001510:intron 35.0%
!!! TTGCTTTTTGAGCTGCTATT+TGG - chr6_4:85312679-85312698 None:intergenic 35.0%
TGATGATCATGAAGTTGAGA+AGG + chr6_4:85312723-85312742 Msa1001510:CDS 35.0%
AACAAAAAGATGTGCCAGAT+TGG + chr6_4:85312842-85312861 Msa1001510:CDS 35.0%
AGGGTCCAATTCAAATAACA+TGG + chr6_4:85312954-85312973 Msa1001510:CDS 35.0%
GCACACCAACACTATTTAAT+TGG - chr6_4:85310958-85310977 None:intergenic 35.0%
CACAAAAGAACTTATGTGTC+TGG - chr6_4:85311034-85311053 None:intergenic 35.0%
!! GTTTGTGTCGGTTCAAAAAA+TGG + chr6_4:85311129-85311148 Msa1001510:exon 35.0%
GAAACATAGGCCATAATTCA+AGG + chr6_4:85311173-85311192 Msa1001510:CDS 35.0%
!!! AGAGGAAGTAGTAGTTTTGT+TGG + chr6_4:85311211-85311230 Msa1001510:CDS 35.0%
GAAGAAGAAAAGGAAGAAGT+AGG - chr6_4:85311334-85311353 None:intergenic 35.0%
AATAGAGGCTCTATCATCAA+TGG - chr6_4:85311365-85311384 None:intergenic 35.0%
! ATTGATGATAGAGCCTCTAT+TGG + chr6_4:85311364-85311383 Msa1001510:CDS 35.0%
TCATAGTTCGTACACAAGAA+AGG + chr6_4:85311404-85311423 Msa1001510:CDS 35.0%
CGTACACAAGAAAGGAAAAA+AGG + chr6_4:85311412-85311431 Msa1001510:CDS 35.0%
GAGCATAGAAAATTCCTTGT+TGG + chr6_4:85311699-85311718 Msa1001510:CDS 35.0%
ACGTCTCTTCTTCTTATTCA+AGG - chr6_4:85311838-85311857 None:intergenic 35.0%
ACGCATTTGGATGTATAATC+CGG + chr6_4:85312144-85312163 Msa1001510:intron 35.0%
TGACTAAATTCAGACGTGAA+TGG + chr6_4:85312194-85312213 Msa1001510:intron 35.0%
TCAAGTGATCTTAGAGTGAT+CGG - chr6_4:85312321-85312340 None:intergenic 35.0%
AGAGTTCCAAATATGCTTAG+AGG + chr6_4:85312388-85312407 Msa1001510:intron 35.0%
!!! TTGCTTTTTGAGCTGCTATT+TGG - chr6_4:85312679-85312698 None:intergenic 35.0%
TGATGATCATGAAGTTGAGA+AGG + chr6_4:85312723-85312742 Msa1001510:CDS 35.0%
AACAAAAAGATGTGCCAGAT+TGG + chr6_4:85312842-85312861 Msa1001510:CDS 35.0%
AGGGTCCAATTCAAATAACA+TGG + chr6_4:85312954-85312973 Msa1001510:CDS 35.0%
GCGGTGGAATTTGTGAAAAT+TGG - chr6_4:85310983-85311002 None:intergenic 40.0%
TGCTCACATTGTGGAAACAT+AGG + chr6_4:85311160-85311179 Msa1001510:CDS 40.0%
GTAGGAGAAGAAGATGAAGT+AGG - chr6_4:85311316-85311335 None:intergenic 40.0%
ACGTTCAAGTCTCTTTGACA+TGG + chr6_4:85311854-85311873 Msa1001510:CDS 40.0%
! AAAAGCACACTACTCCGAAT+TGG + chr6_4:85312111-85312130 Msa1001510:intron 40.0%
CAAGTGATCTTAGAGTGATC+GGG - chr6_4:85312320-85312339 None:intergenic 40.0%
GCGGTGGAATTTGTGAAAAT+TGG - chr6_4:85310983-85311002 None:intergenic 40.0%
TGCTCACATTGTGGAAACAT+AGG + chr6_4:85311160-85311179 Msa1001510:CDS 40.0%
GTAGGAGAAGAAGATGAAGT+AGG - chr6_4:85311316-85311335 None:intergenic 40.0%
ACGTTCAAGTCTCTTTGACA+TGG + chr6_4:85311854-85311873 Msa1001510:CDS 40.0%
! AAAAGCACACTACTCCGAAT+TGG + chr6_4:85312111-85312130 Msa1001510:intron 40.0%
CAAGTGATCTTAGAGTGATC+GGG - chr6_4:85312320-85312339 None:intergenic 40.0%
! TGATGATTGATGGGTGTGAG+CGG - chr6_4:85311002-85311021 None:intergenic 45.0%
CTATGCTCTTCTTCTGTCCA+TGG - chr6_4:85311687-85311706 None:intergenic 45.0%
CAAGCTTCTCAAGTCCAACA+AGG - chr6_4:85311716-85311735 None:intergenic 45.0%
CTTGTTGGACTTGAGAAGCT+TGG + chr6_4:85311714-85311733 Msa1001510:CDS 45.0%
GGACTTGAGAAGCTTGGAAA+AGG + chr6_4:85311720-85311739 Msa1001510:CDS 45.0%
AGAAGCTTGGAAAAGGAGAC+TGG + chr6_4:85311727-85311746 Msa1001510:CDS 45.0%
CACACTACTCCGAATTGGAA+AGG + chr6_4:85312116-85312135 Msa1001510:intron 45.0%
CTTAACACACACACCCCTTA+TGG - chr6_4:85312370-85312389 None:intergenic 45.0%
AGCAGCTCAAAAAGCAAGTC+AGG + chr6_4:85312682-85312701 Msa1001510:CDS 45.0%
GTTAGAGGATGTAGCCAATC+TGG - chr6_4:85312859-85312878 None:intergenic 45.0%
ACCTGATCTTGAGCTAACTC+TGG + chr6_4:85312930-85312949 Msa1001510:CDS 45.0%
ATCTTGAGCTAACTCTGGCA+GGG + chr6_4:85312935-85312954 Msa1001510:CDS 45.0%
! TGATGATTGATGGGTGTGAG+CGG - chr6_4:85311002-85311021 None:intergenic 45.0%
CTATGCTCTTCTTCTGTCCA+TGG - chr6_4:85311687-85311706 None:intergenic 45.0%
CAAGCTTCTCAAGTCCAACA+AGG - chr6_4:85311716-85311735 None:intergenic 45.0%
CTTGTTGGACTTGAGAAGCT+TGG + chr6_4:85311714-85311733 Msa1001510:CDS 45.0%
GGACTTGAGAAGCTTGGAAA+AGG + chr6_4:85311720-85311739 Msa1001510:CDS 45.0%
AGAAGCTTGGAAAAGGAGAC+TGG + chr6_4:85311727-85311746 Msa1001510:CDS 45.0%
CACACTACTCCGAATTGGAA+AGG + chr6_4:85312116-85312135 Msa1001510:intron 45.0%
CTTAACACACACACCCCTTA+TGG - chr6_4:85312370-85312389 None:intergenic 45.0%
AGCAGCTCAAAAAGCAAGTC+AGG + chr6_4:85312682-85312701 Msa1001510:CDS 45.0%
GTTAGAGGATGTAGCCAATC+TGG - chr6_4:85312859-85312878 None:intergenic 45.0%
ACCTGATCTTGAGCTAACTC+TGG + chr6_4:85312930-85312949 Msa1001510:CDS 45.0%
ATCTTGAGCTAACTCTGGCA+GGG + chr6_4:85312935-85312954 Msa1001510:CDS 45.0%
!! TATATATATATAATTATGAA+AGG - chr6_4:85311920-85311939 None:intergenic 5.0%
!! TATATATATATAATTATGAA+AGG - chr6_4:85311920-85311939 None:intergenic 5.0%
!! GGCAGAAAGTGCTCACATTG+TGG + chr6_4:85311151-85311170 Msa1001510:CDS 50.0%
AGCTTGGAAAAGGAGACTGG+AGG + chr6_4:85311730-85311749 Msa1001510:CDS 50.0%
GCTTGGAAAAGGAGACTGGA+GGG + chr6_4:85311731-85311750 Msa1001510:CDS 50.0%
CTTGGAAAAGGAGACTGGAG+GGG + chr6_4:85311732-85311751 Msa1001510:CDS 50.0%
! GCATGGCTTGCAACTTGTGT+TGG - chr6_4:85311789-85311808 None:intergenic 50.0%
GCGTTCGTGCCTTTCCAATT+CGG - chr6_4:85312128-85312147 None:intergenic 50.0%
! TGGAAAGGCACGAACGCATT+TGG + chr6_4:85312131-85312150 Msa1001510:intron 50.0%
GCCAGAGTTAGCTCAAGATC+AGG - chr6_4:85312934-85312953 None:intergenic 50.0%
GATCTTGAGCTAACTCTGGC+AGG + chr6_4:85312934-85312953 Msa1001510:CDS 50.0%
!! GGCAGAAAGTGCTCACATTG+TGG + chr6_4:85311151-85311170 Msa1001510:CDS 50.0%
AGCTTGGAAAAGGAGACTGG+AGG + chr6_4:85311730-85311749 Msa1001510:CDS 50.0%
GCTTGGAAAAGGAGACTGGA+GGG + chr6_4:85311731-85311750 Msa1001510:CDS 50.0%
CTTGGAAAAGGAGACTGGAG+GGG + chr6_4:85311732-85311751 Msa1001510:CDS 50.0%
! GCATGGCTTGCAACTTGTGT+TGG - chr6_4:85311789-85311808 None:intergenic 50.0%
GCGTTCGTGCCTTTCCAATT+CGG - chr6_4:85312128-85312147 None:intergenic 50.0%
! TGGAAAGGCACGAACGCATT+TGG + chr6_4:85312131-85312150 Msa1001510:intron 50.0%
GCCAGAGTTAGCTCAAGATC+AGG - chr6_4:85312934-85312953 None:intergenic 50.0%
GATCTTGAGCTAACTCTGGC+AGG + chr6_4:85312934-85312953 Msa1001510:CDS 50.0%
! TGATTGATGGGTGTGAGCGG+TGG - chr6_4:85310999-85311018 None:intergenic 55.0%
! TGATTGATGGGTGTGAGCGG+TGG - chr6_4:85310999-85311018 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 85310907 85313232 85310907 ID=Msa1001510;Name=Msa1001510
chr6_4 mRNA 85310907 85313232 85310907 ID=Msa1001510-mRNA-1;Parent=Msa1001510;Name=Msa1001510-mRNA-1;_AED=0.04;_eAED=0.04;_QI=241|1|1|1|1|1|3|200|290
chr6_4 exon 85310907 85311433 85310907 ID=Msa1001510-mRNA-1:exon:16743;Parent=Msa1001510-mRNA-1
chr6_4 exon 85311679 85311875 85311679 ID=Msa1001510-mRNA-1:exon:16744;Parent=Msa1001510-mRNA-1
chr6_4 exon 85312643 85313232 85312643 ID=Msa1001510-mRNA-1:exon:16745;Parent=Msa1001510-mRNA-1
chr6_4 five_prime_UTR 85310907 85311147 85310907 ID=Msa1001510-mRNA-1:five_prime_utr;Parent=Msa1001510-mRNA-1
chr6_4 CDS 85311148 85311433 85311148 ID=Msa1001510-mRNA-1:cds;Parent=Msa1001510-mRNA-1
chr6_4 CDS 85311679 85311875 85311679 ID=Msa1001510-mRNA-1:cds;Parent=Msa1001510-mRNA-1
chr6_4 CDS 85312643 85313032 85312643 ID=Msa1001510-mRNA-1:cds;Parent=Msa1001510-mRNA-1
chr6_4 three_prime_UTR 85313033 85313232 85313033 ID=Msa1001510-mRNA-1:three_prime_utr;Parent=Msa1001510-mRNA-1
Gene Sequence

>Msa1001510

ATGGGCAGAAAGTGCTCACATTGTGGAAACATAGGCCATAATTCAAGGACTTGTAATTCATTAAGAGGAAGTAGTAGTTTTGTTGGAGTTAGACTTTTTGGTGTTCAACTTGATTTATCTTCTTCTTGTGTTTCCATGAAAAAGAGTTTTAGCATGGATTCTTTTCCTACTTCATCTTCTTCTCCTACTTCTTCCTTTTCTTCTTCAAGATTATCCATTGATGATAGAGCCTCTATTGGTTATCTTTCAGATGGTCTCATAGTTCGTACACAAGAAAGGAAAAAAGGAGTTCCATGGACAGAAGAAGAGCATAGAAAATTCCTTGTTGGACTTGAGAAGCTTGGAAAAGGAGACTGGAGGGGTATCTCTAGAAACTATGTGACTACAAGAACACCAACACAAGTTGCAAGCCATGCTCAAAAGTATTTTATTCGACTTGCAACCTTGAATAAGAAGAAGAGACGTTCAAGTCTCTTTGACATGGTTGGTAGTGGCAAGACAAACAAAGCAGTTGATCCAAATAGCAGCTCAAAAAGCAAGTCAGGTGATTCAGTTTGTAGACATGATGATCATGAAGTTGAGAAGGATGAAACTTTGTCACTACTAATAAACTCACTTCAACAACAAACCAAATCAGATGATTATGATATGCAAAAAATTGAAGATGATTCTGAAGAAGCAAAACAAAAAGATGTGCCAGATTGGCTACATCCTCTAACAAAGTCTTTAAATATGACATTGGTGATACCAAATTCTTCAAATGTAGCATCACCTGATCTTGAGCTAACTCTGGCAGGGTCCAATTCAAATAACATGGAACAAGATAAAACATCATCAAGTTCATTTCTTATTGGGCCAATTAGTGTGACTTAA
Protein sequence

>Msa1001510

MGRKCSHCGNIGHNSRTCNSLRGSSSFVGVRLFGVQLDLSSSCVSMKKSFSMDSFPTSSSSPTSSFSSSRLSIDDRASIGYLSDGLIVRTQERKKGVPWTEEEHRKFLVGLEKLGKGDWRGISRNYVTTRTPTQVASHAQKYFIRLATLNKKKRRSSLFDMVGSGKTNKAVDPNSSSKSKSGDSVCRHDDHEVEKDETLSLLINSLQQQTKSDDYDMQKIEDDSEEAKQKDVPDWLHPLTKSLNMTLVIPNSSNVASPDLELTLAGSNSNNMEQDKTSSSSFLIGPISVT*