AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1003820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003820 MtrunA17_Chr7g0214511 83.946 299 22 5 10 302 9 287 1.52e-169 471
Msa1003820 MtrunA17_Chr1g0174001 60.309 194 58 8 118 302 12 195 1.88e-63 198
Msa1003820 MtrunA17_Chr1g0169521 49.808 261 83 9 60 302 86 316 1.53e-61 197
Msa1003820 MtrunA17_Chr1g0169561 49.624 266 83 12 56 302 83 316 4.21e-60 194
Msa1003820 MtrunA17_Chr1g0193731 47.576 330 81 17 16 302 15 295 2.63e-58 189
Msa1003820 MtrunA17_Chr1g0180581 44.444 288 84 11 15 302 9 220 4.40e-51 167
Msa1003820 MtrunA17_Chr1g0174871 35.907 259 121 11 72 301 42 284 1.83e-22 94.7
Msa1003820 MtrunA17_Chr1g0174861 37.363 182 88 9 93 268 63 224 2.10e-20 88.2
Msa1003820 MtrunA17_Chr1g0180571 78.431 51 11 0 247 297 22 72 3.71e-19 82.0
Msa1003820 MtrunA17_Chr1g0172861 35.055 271 102 15 72 302 42 278 1.54e-16 78.2
Msa1003820 MtrunA17_Chr1g0172961 32.411 253 101 13 72 284 42 264 1.75e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAAGGTGTTACTGAATT+AGG 0.100666 7_1:-895582 Msa1003820:CDS
TGAATCACCGTGGCGCTAAA+AGG 0.215611 7_1:-895830 Msa1003820:CDS
TCGACGCGGCCGGCGTTTCT+TGG 0.252455 7_1:+895956 None:intergenic
TTCTTGGTGTTGTAGAATAT+TGG 0.276502 7_1:+895972 None:intergenic
CTCTTCAATAGCATGATTAG+AGG 0.355993 7_1:+895614 None:intergenic
ATGAAGGTGTTACTGAATTA+GGG 0.357850 7_1:-895581 Msa1003820:CDS
TTCTGTTCTTGGTAATCATA+AGG 0.363019 7_1:-896174 Msa1003820:CDS
ATCGCTCCCTTCAAAATTCC+AGG 0.381362 7_1:-896325 Msa1003820:CDS
CAAAGCATTGAATGGACATA+AGG 0.388435 7_1:-896069 Msa1003820:CDS
TATATCTCGCGGAGGTTATG+AGG 0.390309 7_1:-895874 Msa1003820:CDS
AGTACTTTGAACAGTCAATA+TGG 0.393472 7_1:+896000 None:intergenic
AAAACTTTCTTGCAATATGA+TGG 0.405590 7_1:-895805 Msa1003820:CDS
GCATCATTAGTTGTTGAAGA+TGG 0.412853 7_1:+896245 None:intergenic
ACTTCTAACAAAGCATTGAA+TGG 0.414800 7_1:-896077 Msa1003820:CDS
ATAGCATGATTAGAGGTAGA+AGG 0.440842 7_1:+895621 None:intergenic
GAAGATGGTGGTGCAGTTTG+TGG 0.454649 7_1:+896035 None:intergenic
GAATAGGGTTTGAGTAATCA+AGG 0.456225 7_1:+896285 None:intergenic
AAAGCATTGAATGGACATAA+GGG 0.470952 7_1:-896068 Msa1003820:CDS
TTCTTCTTCTTCGACGCGGC+CGG 0.486419 7_1:+895946 None:intergenic
GAATCACCGTGGCGCTAAAA+GGG 0.488695 7_1:-895829 Msa1003820:CDS
TAATCAAGGAAACGGAAAGA+AGG 0.496330 7_1:+896299 None:intergenic
GTCAATATGGCTCTGTTCAA+AGG 0.497478 7_1:+896013 None:intergenic
TCTTCAACAACTAATGATGC+TGG 0.499426 7_1:-896242 Msa1003820:CDS
GGAGCGATGATAAACATCAT+TGG 0.502169 7_1:+896340 None:intergenic
GAAGAGAAGAAGATTAATGA+AGG 0.510676 7_1:-895597 Msa1003820:CDS
TCAAACATCACAAGATTGCA+AGG 0.513757 7_1:-895646 Msa1003820:CDS
CTCATGTGATGTTTGCAACA+AGG 0.516766 7_1:-896105 Msa1003820:CDS
TTGGTGTTGTAGAATATTGG+CGG 0.527171 7_1:+895975 None:intergenic
TTGAGGTTCAAGATTCCTAA+AGG 0.528982 7_1:-895678 Msa1003820:CDS
AAGATGGTGGTGCAGTTTGT+GGG 0.535375 7_1:+896036 None:intergenic
GAAACGGAAAGAAGGGTACC+TGG 0.540917 7_1:+896307 None:intergenic
TAGCCACAATGAAAAGAAGT+TGG 0.545096 7_1:-895703 Msa1003820:CDS
GGTTTGAGTAATCAAGGAAA+CGG 0.550662 7_1:+896291 None:intergenic
TTTGGTTCATGATCATGAGA+AGG 0.562074 7_1:+896365 None:intergenic
TCATGATCATGAGAAGGAGA+TGG 0.576941 7_1:+896371 None:intergenic
TGGCTCTGTTCAAAGGAAGA+TGG 0.582600 7_1:+896020 None:intergenic
AATCAAGGAAACGGAAAGAA+GGG 0.583538 7_1:+896300 None:intergenic
TCATCTTCAAAATCATCACT+TGG 0.584649 7_1:+895522 None:intergenic
CAAGGTGTGTTTCTAACAAG+TGG 0.594003 7_1:-895753 Msa1003820:CDS
ATGAAAAGAAGTTGGTGTTG+AGG 0.594249 7_1:-895695 Msa1003820:CDS
ACAACGTGAAGAAGATCGTA+TGG 0.595118 7_1:-896208 Msa1003820:CDS
ATATCTCGCGGAGGTTATGA+GGG 0.614101 7_1:-895873 Msa1003820:CDS
ACTTTGTTGCATATATCTCG+CGG 0.675938 7_1:-895885 Msa1003820:CDS
TCTACAACACCAAGAAACGC+CGG 0.683622 7_1:-895965 Msa1003820:CDS
TATCTTCTTCTTCTTCGACG+CGG 0.720402 7_1:+895942 None:intergenic
CTCTGTTCAAAGGAAGATGG+TGG 0.725506 7_1:+896023 None:intergenic
TTGTTGCATATATCTCGCGG+AGG 0.742758 7_1:-895882 Msa1003820:CDS
AATATCGATGTGAATCACCG+TGG 0.797456 7_1:-895840 Msa1003820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTATTCTGTTCT+TGG - chr7_1:895725-895744 Msa1003820:CDS 20.0%
!!! TTTTTTTCTTATTCTGTTCT+TGG - chr7_1:895725-895744 Msa1003820:CDS 20.0%
!!! TGATAAACATCATTGGTTTT+TGG + chr7_1:895566-895585 None:intergenic 25.0%
!!! TTTTGAGTTCATTCTGAATA+GGG + chr7_1:895643-895662 None:intergenic 25.0%
!!! TTTTTGAGTTCATTCTGAAT+AGG + chr7_1:895644-895663 None:intergenic 25.0%
! AAAACTTTCTTGCAATATGA+TGG - chr7_1:896105-896124 Msa1003820:CDS 25.0%
!!! TGATAAACATCATTGGTTTT+TGG + chr7_1:895566-895585 None:intergenic 25.0%
!!! TTTTGAGTTCATTCTGAATA+GGG + chr7_1:895643-895662 None:intergenic 25.0%
!!! TTTTTGAGTTCATTCTGAAT+AGG + chr7_1:895644-895663 None:intergenic 25.0%
! AAAACTTTCTTGCAATATGA+TGG - chr7_1:896105-896124 Msa1003820:CDS 25.0%
! TTCTGTTCTTGGTAATCATA+AGG - chr7_1:895736-895755 Msa1003820:CDS 30.0%
!! ACTTCTAACAAAGCATTGAA+TGG - chr7_1:895833-895852 Msa1003820:CDS 30.0%
!! AAAGCATTGAATGGACATAA+GGG - chr7_1:895842-895861 Msa1003820:CDS 30.0%
AGTACTTTGAACAGTCAATA+TGG + chr7_1:895913-895932 None:intergenic 30.0%
!! TTCTTGGTGTTGTAGAATAT+TGG + chr7_1:895941-895960 None:intergenic 30.0%
GTTTGAAAAATCTTGCCTTT+AGG + chr7_1:896250-896269 None:intergenic 30.0%
GAAGAGAAGAAGATTAATGA+AGG - chr7_1:896313-896332 Msa1003820:CDS 30.0%
!! AATGAAGGTGTTACTGAATT+AGG - chr7_1:896328-896347 Msa1003820:CDS 30.0%
!! ATGAAGGTGTTACTGAATTA+GGG - chr7_1:896329-896348 Msa1003820:CDS 30.0%
TCATCTTCAAAATCATCACT+TGG + chr7_1:896391-896410 None:intergenic 30.0%
! TTCTGTTCTTGGTAATCATA+AGG - chr7_1:895736-895755 Msa1003820:CDS 30.0%
!! ACTTCTAACAAAGCATTGAA+TGG - chr7_1:895833-895852 Msa1003820:CDS 30.0%
!! AAAGCATTGAATGGACATAA+GGG - chr7_1:895842-895861 Msa1003820:CDS 30.0%
AGTACTTTGAACAGTCAATA+TGG + chr7_1:895913-895932 None:intergenic 30.0%
!! TTCTTGGTGTTGTAGAATAT+TGG + chr7_1:895941-895960 None:intergenic 30.0%
GTTTGAAAAATCTTGCCTTT+AGG + chr7_1:896250-896269 None:intergenic 30.0%
GAAGAGAAGAAGATTAATGA+AGG - chr7_1:896313-896332 Msa1003820:CDS 30.0%
!! AATGAAGGTGTTACTGAATT+AGG - chr7_1:896328-896347 Msa1003820:CDS 30.0%
!! ATGAAGGTGTTACTGAATTA+GGG - chr7_1:896329-896348 Msa1003820:CDS 30.0%
TCATCTTCAAAATCATCACT+TGG + chr7_1:896391-896410 None:intergenic 30.0%
! TTTGGTTCATGATCATGAGA+AGG + chr7_1:895548-895567 None:intergenic 35.0%
AATCAAGGAAACGGAAAGAA+GGG + chr7_1:895613-895632 None:intergenic 35.0%
TAATCAAGGAAACGGAAAGA+AGG + chr7_1:895614-895633 None:intergenic 35.0%
GGTTTGAGTAATCAAGGAAA+CGG + chr7_1:895622-895641 None:intergenic 35.0%
GAATAGGGTTTGAGTAATCA+AGG + chr7_1:895628-895647 None:intergenic 35.0%
GCATCATTAGTTGTTGAAGA+TGG + chr7_1:895668-895687 None:intergenic 35.0%
TCTTCAACAACTAATGATGC+TGG - chr7_1:895668-895687 Msa1003820:CDS 35.0%
!! CAAAGCATTGAATGGACATA+AGG - chr7_1:895841-895860 Msa1003820:CDS 35.0%
!! TTGGTGTTGTAGAATATTGG+CGG + chr7_1:895938-895957 None:intergenic 35.0%
ACTTTGTTGCATATATCTCG+CGG - chr7_1:896025-896044 Msa1003820:CDS 35.0%
TGATGAGAAGAAGAAGAAGA+AGG - chr7_1:896132-896151 Msa1003820:CDS 35.0%
AAGAAGAAGAAGAAGGAACA+AGG - chr7_1:896139-896158 Msa1003820:CDS 35.0%
TAGCCACAATGAAAAGAAGT+TGG - chr7_1:896207-896226 Msa1003820:CDS 35.0%
! ACACCAACTTCTTTTCATTG+TGG + chr7_1:896213-896232 None:intergenic 35.0%
!! ATGAAAAGAAGTTGGTGTTG+AGG - chr7_1:896215-896234 Msa1003820:CDS 35.0%
TTGAGGTTCAAGATTCCTAA+AGG - chr7_1:896232-896251 Msa1003820:CDS 35.0%
TCAAACATCACAAGATTGCA+AGG - chr7_1:896264-896283 Msa1003820:CDS 35.0%
ATAGCATGATTAGAGGTAGA+AGG + chr7_1:896292-896311 None:intergenic 35.0%
CTCTTCAATAGCATGATTAG+AGG + chr7_1:896299-896318 None:intergenic 35.0%
! TTTGGTTCATGATCATGAGA+AGG + chr7_1:895548-895567 None:intergenic 35.0%
AATCAAGGAAACGGAAAGAA+GGG + chr7_1:895613-895632 None:intergenic 35.0%
TAATCAAGGAAACGGAAAGA+AGG + chr7_1:895614-895633 None:intergenic 35.0%
GGTTTGAGTAATCAAGGAAA+CGG + chr7_1:895622-895641 None:intergenic 35.0%
GAATAGGGTTTGAGTAATCA+AGG + chr7_1:895628-895647 None:intergenic 35.0%
GCATCATTAGTTGTTGAAGA+TGG + chr7_1:895668-895687 None:intergenic 35.0%
TCTTCAACAACTAATGATGC+TGG - chr7_1:895668-895687 Msa1003820:CDS 35.0%
!! CAAAGCATTGAATGGACATA+AGG - chr7_1:895841-895860 Msa1003820:CDS 35.0%
!! TTGGTGTTGTAGAATATTGG+CGG + chr7_1:895938-895957 None:intergenic 35.0%
ACTTTGTTGCATATATCTCG+CGG - chr7_1:896025-896044 Msa1003820:CDS 35.0%
TGATGAGAAGAAGAAGAAGA+AGG - chr7_1:896132-896151 Msa1003820:CDS 35.0%
AAGAAGAAGAAGAAGGAACA+AGG - chr7_1:896139-896158 Msa1003820:CDS 35.0%
TAGCCACAATGAAAAGAAGT+TGG - chr7_1:896207-896226 Msa1003820:CDS 35.0%
! ACACCAACTTCTTTTCATTG+TGG + chr7_1:896213-896232 None:intergenic 35.0%
!! ATGAAAAGAAGTTGGTGTTG+AGG - chr7_1:896215-896234 Msa1003820:CDS 35.0%
TTGAGGTTCAAGATTCCTAA+AGG - chr7_1:896232-896251 Msa1003820:CDS 35.0%
TCAAACATCACAAGATTGCA+AGG - chr7_1:896264-896283 Msa1003820:CDS 35.0%
ATAGCATGATTAGAGGTAGA+AGG + chr7_1:896292-896311 None:intergenic 35.0%
CTCTTCAATAGCATGATTAG+AGG + chr7_1:896299-896318 None:intergenic 35.0%
TCATGATCATGAGAAGGAGA+TGG + chr7_1:895542-895561 None:intergenic 40.0%
GGAGCGATGATAAACATCAT+TGG + chr7_1:895573-895592 None:intergenic 40.0%
!! AGGGTACCTGGAATTTTGAA+GGG + chr7_1:895594-895613 None:intergenic 40.0%
!!! AAGGGTACCTGGAATTTTGA+AGG + chr7_1:895595-895614 None:intergenic 40.0%
ACAACGTGAAGAAGATCGTA+TGG - chr7_1:895702-895721 Msa1003820:CDS 40.0%
CTCATGTGATGTTTGCAACA+AGG - chr7_1:895805-895824 Msa1003820:CDS 40.0%
! GTCAATATGGCTCTGTTCAA+AGG + chr7_1:895900-895919 None:intergenic 40.0%
TATCTTCTTCTTCTTCGACG+CGG + chr7_1:895971-895990 None:intergenic 40.0%
AATATCGATGTGAATCACCG+TGG - chr7_1:896070-896089 Msa1003820:CDS 40.0%
! CAAGGTGTGTTTCTAACAAG+TGG - chr7_1:896157-896176 Msa1003820:CDS 40.0%
TCATGATCATGAGAAGGAGA+TGG + chr7_1:895542-895561 None:intergenic 40.0%
GGAGCGATGATAAACATCAT+TGG + chr7_1:895573-895592 None:intergenic 40.0%
!! AGGGTACCTGGAATTTTGAA+GGG + chr7_1:895594-895613 None:intergenic 40.0%
!!! AAGGGTACCTGGAATTTTGA+AGG + chr7_1:895595-895614 None:intergenic 40.0%
ACAACGTGAAGAAGATCGTA+TGG - chr7_1:895702-895721 Msa1003820:CDS 40.0%
CTCATGTGATGTTTGCAACA+AGG - chr7_1:895805-895824 Msa1003820:CDS 40.0%
! GTCAATATGGCTCTGTTCAA+AGG + chr7_1:895900-895919 None:intergenic 40.0%
TATCTTCTTCTTCTTCGACG+CGG + chr7_1:895971-895990 None:intergenic 40.0%
AATATCGATGTGAATCACCG+TGG - chr7_1:896070-896089 Msa1003820:CDS 40.0%
! CAAGGTGTGTTTCTAACAAG+TGG - chr7_1:896157-896176 Msa1003820:CDS 40.0%
ATCGCTCCCTTCAAAATTCC+AGG - chr7_1:895585-895604 Msa1003820:CDS 45.0%
! AAGATGGTGGTGCAGTTTGT+GGG + chr7_1:895877-895896 None:intergenic 45.0%
CTCTGTTCAAAGGAAGATGG+TGG + chr7_1:895890-895909 None:intergenic 45.0%
! TGGCTCTGTTCAAAGGAAGA+TGG + chr7_1:895893-895912 None:intergenic 45.0%
TCTACAACACCAAGAAACGC+CGG - chr7_1:895945-895964 Msa1003820:CDS 45.0%
TTGTTGCATATATCTCGCGG+AGG - chr7_1:896028-896047 Msa1003820:CDS 45.0%
TATATCTCGCGGAGGTTATG+AGG - chr7_1:896036-896055 Msa1003820:CDS 45.0%
ATATCTCGCGGAGGTTATGA+GGG - chr7_1:896037-896056 Msa1003820:CDS 45.0%
! GTTTTTCCCTTTTAGCGCCA+CGG + chr7_1:896090-896109 None:intergenic 45.0%
ATCGCTCCCTTCAAAATTCC+AGG - chr7_1:895585-895604 Msa1003820:CDS 45.0%
! AAGATGGTGGTGCAGTTTGT+GGG + chr7_1:895877-895896 None:intergenic 45.0%
CTCTGTTCAAAGGAAGATGG+TGG + chr7_1:895890-895909 None:intergenic 45.0%
! TGGCTCTGTTCAAAGGAAGA+TGG + chr7_1:895893-895912 None:intergenic 45.0%
TCTACAACACCAAGAAACGC+CGG - chr7_1:895945-895964 Msa1003820:CDS 45.0%
TTGTTGCATATATCTCGCGG+AGG - chr7_1:896028-896047 Msa1003820:CDS 45.0%
TATATCTCGCGGAGGTTATG+AGG - chr7_1:896036-896055 Msa1003820:CDS 45.0%
ATATCTCGCGGAGGTTATGA+GGG - chr7_1:896037-896056 Msa1003820:CDS 45.0%
! GTTTTTCCCTTTTAGCGCCA+CGG + chr7_1:896090-896109 None:intergenic 45.0%
!! GAAACGGAAAGAAGGGTACC+TGG + chr7_1:895606-895625 None:intergenic 50.0%
! GAAGATGGTGGTGCAGTTTG+TGG + chr7_1:895878-895897 None:intergenic 50.0%
TGAATCACCGTGGCGCTAAA+AGG - chr7_1:896080-896099 Msa1003820:CDS 50.0%
GAATCACCGTGGCGCTAAAA+GGG - chr7_1:896081-896100 Msa1003820:CDS 50.0%
!! GAAACGGAAAGAAGGGTACC+TGG + chr7_1:895606-895625 None:intergenic 50.0%
! GAAGATGGTGGTGCAGTTTG+TGG + chr7_1:895878-895897 None:intergenic 50.0%
TGAATCACCGTGGCGCTAAA+AGG - chr7_1:896080-896099 Msa1003820:CDS 50.0%
GAATCACCGTGGCGCTAAAA+GGG - chr7_1:896081-896100 Msa1003820:CDS 50.0%
TTCTTCTTCTTCGACGCGGC+CGG + chr7_1:895967-895986 None:intergenic 55.0%
TTCTTCTTCTTCGACGCGGC+CGG + chr7_1:895967-895986 None:intergenic 55.0%
TCGACGCGGCCGGCGTTTCT+TGG + chr7_1:895957-895976 None:intergenic 70.0%
TCGACGCGGCCGGCGTTTCT+TGG + chr7_1:895957-895976 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 895512 896420 895512 ID=Msa1003820;Name=Msa1003820
chr7_1 mRNA 895512 896420 895512 ID=Msa1003820-mRNA-1;Parent=Msa1003820;Name=Msa1003820-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|302
chr7_1 exon 895512 896420 895512 ID=Msa1003820-mRNA-1:exon:434;Parent=Msa1003820-mRNA-1
chr7_1 CDS 895512 896420 895512 ID=Msa1003820-mRNA-1:cds;Parent=Msa1003820-mRNA-1
Gene Sequence

>Msa1003820

ATGTATTCTTCTTCTTCTTCTTCATCTCCATCTCCTTCTCATGATCATGAACCAAAAACCAATGATGTTTATCATCGCTCCCTTCAAAATTCCAGGTACCCTTCTTTCCGTTTCCTTGATTACTCAAACCCTATTCAGAATGAACTCAAAAATCCATCTTCAACAACTAATGATGCTGGAAAACAACAGAACAACGTGAAGAAGATCGTATGGTTTTTTTCTTATTCTGTTCTTGGTAATCATAAGGATGTTGAAAAACAGAACAAACATACATACTCATACTCATACTCATACTCATGTGATGTTTGCAACAAGGTTTTTACTTCTAACAAAGCATTGAATGGACATAAGGGTGTTATTCACCCACAAACTGCACCACCATCTTCCTTTGAACAGAGCCATATTGACTGTTCAAAGTACTTGCCGCCAATATTCTACAACACCAAGAAACGCCGGCCGCGTCGAAGAAGAAGAAGATATATGAACATTGATGATGATGTGATTGCTGCTGAAACTTTGTTGCATATATCTCGCGGAGGTTATGAGGGATCATCTGTCAATATCGATGTGAATCACCGTGGCGCTAAAAGGGAAAAACTTTCTTGCAATATGATGGATGATGATGAGAAGAAGAAGAAGAAGGAACAAGGTGTGTTTCTAACAAGTGGCGATATTGATGAAACATTGAAAGATAATAGCCACAATGAAAAGAAGTTGGTGTTGAGGTTCAAGATTCCTAAAGGCAAGATTTTTCAAACATCACAAGATTGCAAGGAACCTTCTACCTCTAATCATGCTATTGAAGAGAAGAAGATTAATGAAGGTGTTACTGAATTAGGGTCAAAAGTTGTGAAGAATTTTGATTTGAACGAGCTTCCAAGTGATGATTTTGAAGATGAAACTAATTAG

Protein sequence

>Msa1003820

MYSSSSSSSPSPSHDHEPKTNDVYHRSLQNSRYPSFRFLDYSNPIQNELKNPSSTTNDAGKQQNNVKKIVWFFSYSVLGNHKDVEKQNKHTYSYSYSYSCDVCNKVFTSNKALNGHKGVIHPQTAPPSSFEQSHIDCSKYLPPIFYNTKKRRPRRRRRRYMNIDDDVIAAETLLHISRGGYEGSSVNIDVNHRGAKREKLSCNMMDDDEKKKKKEQGVFLTSGDIDETLKDNSHNEKKLVLRFKIPKGKIFQTSQDCKEPSTSNHAIEEKKINEGVTELGSKVVKNFDLNELPSDDFEDETN*