Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1005610 | AES78253.1 | 89.758 | 537 | 54 | 1 | 1 | 537 | 18 | 553 | 0.0 | 934 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1005610 | sp|Q9ZWJ9|ARR2_ARATH | 36.820 | 239 | 120 | 6 | 6 | 220 | 60 | 291 | 1.09e-37 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1005610 | G7L033 | 89.758 | 537 | 54 | 1 | 1 | 537 | 18 | 553 | 0.0 | 934 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1005610 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1005610 | MtrunA17_Chr7g0223971 | 83.430 | 519 | 52 | 2 | 52 | 537 | 1 | 518 | 0.0 | 812 |
| Msa1005610 | MtrunA17_Chr7g0223991 | 75.587 | 213 | 32 | 3 | 208 | 418 | 24 | 218 | 8.49e-101 | 303 |
| Msa1005610 | MtrunA17_Chr6g0473731 | 45.602 | 432 | 166 | 13 | 50 | 465 | 10 | 388 | 5.55e-96 | 299 |
| Msa1005610 | MtrunA17_Chr2g0294911 | 39.614 | 207 | 112 | 5 | 3 | 197 | 62 | 267 | 2.27e-37 | 145 |
| Msa1005610 | MtrunA17_Chr4g0067981 | 36.279 | 215 | 104 | 4 | 6 | 197 | 67 | 271 | 1.07e-36 | 144 |
| Msa1005610 | MtrunA17_Chr1g0161431 | 34.286 | 245 | 131 | 7 | 1 | 219 | 42 | 282 | 6.43e-36 | 142 |
| Msa1005610 | MtrunA17_Chr1g0149841 | 35.135 | 222 | 113 | 4 | 1 | 199 | 36 | 249 | 9.05e-35 | 137 |
| Msa1005610 | MtrunA17_Chr8g0376881 | 34.335 | 233 | 134 | 6 | 1 | 215 | 56 | 287 | 2.04e-34 | 136 |
| Msa1005610 | MtrunA17_Chr3g0123131 | 32.950 | 261 | 146 | 7 | 1 | 236 | 44 | 300 | 2.79e-34 | 136 |
| Msa1005610 | MtrunA17_Chr4g0009981 | 29.286 | 280 | 167 | 7 | 15 | 285 | 2 | 259 | 1.15e-33 | 128 |
| Msa1005610 | MtrunA17_Chr3g0137101 | 36.652 | 221 | 115 | 5 | 1 | 199 | 39 | 256 | 2.19e-33 | 134 |
| Msa1005610 | MtrunA17_Chr4g0009901 | 36.000 | 200 | 104 | 4 | 2 | 197 | 45 | 224 | 8.38e-33 | 126 |
| Msa1005610 | MtrunA17_Chr4g0009851 | 30.851 | 282 | 164 | 8 | 13 | 285 | 53 | 312 | 1.40e-32 | 127 |
| Msa1005610 | MtrunA17_Chr7g0275401 | 36.279 | 215 | 117 | 4 | 1 | 197 | 36 | 248 | 1.66e-32 | 126 |
| Msa1005610 | MtrunA17_Chr2g0304841 | 32.489 | 237 | 128 | 5 | 1 | 208 | 49 | 282 | 2.98e-30 | 125 |
| Msa1005610 | MtrunA17_Chr7g0275891 | 31.739 | 230 | 123 | 6 | 1 | 199 | 36 | 262 | 4.15e-30 | 120 |
| Msa1005610 | MtrunA17_Chr6g0471041 | 34.804 | 204 | 118 | 4 | 1 | 203 | 45 | 234 | 2.72e-29 | 120 |
| Msa1005610 | MtrunA17_Chr4g0074411 | 32.569 | 218 | 133 | 3 | 1 | 205 | 49 | 265 | 3.24e-28 | 119 |
| Msa1005610 | MtrunA17_Chr3g0134481 | 32.461 | 191 | 82 | 3 | 2 | 192 | 40 | 183 | 1.24e-27 | 109 |
| Msa1005610 | MtrunA17_Chr1g0149851 | 32.340 | 235 | 113 | 5 | 1 | 197 | 36 | 262 | 1.49e-26 | 113 |
| Msa1005610 | MtrunA17_Chr4g0074401 | 31.390 | 223 | 136 | 4 | 1 | 207 | 48 | 269 | 2.03e-26 | 113 |
| Msa1005610 | MtrunA17_Chr3g0134461 | 34.211 | 190 | 87 | 4 | 2 | 190 | 40 | 192 | 4.59e-26 | 105 |
| Msa1005610 | MtrunA17_Chr4g0074421 | 35.450 | 189 | 108 | 4 | 19 | 195 | 1 | 187 | 8.54e-25 | 102 |
| Msa1005610 | MtrunA17_Chr1g0149831 | 34.184 | 196 | 87 | 6 | 27 | 199 | 7 | 183 | 1.21e-22 | 96.7 |
| Msa1005610 | MtrunA17_Chr4g0009991 | 36.842 | 133 | 61 | 4 | 63 | 190 | 2 | 116 | 2.25e-18 | 81.3 |
| Msa1005610 | MtrunA17_Chr4g0011071 | 28.477 | 151 | 89 | 3 | 20 | 166 | 39 | 174 | 1.39e-17 | 80.9 |
| Msa1005610 | MtrunA17_Chr4g0031961 | 42.478 | 113 | 48 | 5 | 117 | 219 | 101 | 206 | 3.43e-17 | 82.8 |
| Msa1005610 | MtrunA17_Chr7g0255711 | 48.611 | 72 | 36 | 1 | 129 | 200 | 80 | 150 | 1.02e-16 | 80.9 |
| Msa1005610 | MtrunA17_Chr8g0391911 | 28.774 | 212 | 113 | 7 | 20 | 205 | 58 | 257 | 3.68e-14 | 72.8 |
| Msa1005610 | MtrunA17_Chr2g0324891 | 43.956 | 91 | 49 | 2 | 103 | 193 | 125 | 213 | 1.57e-13 | 72.8 |
| Msa1005610 | MtrunA17_Chr4g0040481 | 60.000 | 55 | 21 | 1 | 139 | 193 | 157 | 210 | 2.23e-13 | 72.0 |
| Msa1005610 | MtrunA17_Chr5g0400241 | 52.459 | 61 | 28 | 1 | 139 | 199 | 310 | 369 | 1.86e-12 | 69.7 |
| Msa1005610 | MtrunA17_Chr4g0054981 | 50.000 | 64 | 31 | 1 | 139 | 202 | 309 | 371 | 2.45e-12 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1005610 | AT3G16857.3 | 35.081 | 248 | 118 | 6 | 9 | 220 | 2 | 242 | 2.41e-38 | 149 |
| Msa1005610 | AT3G16857.1 | 35.060 | 251 | 120 | 6 | 6 | 220 | 69 | 312 | 3.62e-38 | 149 |
| Msa1005610 | AT3G16857.2 | 35.060 | 251 | 120 | 6 | 6 | 220 | 69 | 312 | 4.10e-38 | 149 |
| Msa1005610 | AT5G07210.2 | 35.907 | 259 | 121 | 8 | 1 | 217 | 43 | 298 | 7.60e-36 | 142 |
| Msa1005610 | AT5G07210.1 | 35.907 | 259 | 121 | 8 | 1 | 217 | 51 | 306 | 8.88e-36 | 141 |
| Msa1005610 | AT2G25180.1 | 36.019 | 211 | 122 | 3 | 1 | 199 | 44 | 253 | 6.18e-32 | 130 |
| Msa1005610 | AT2G01760.1 | 36.792 | 212 | 110 | 5 | 9 | 197 | 46 | 256 | 1.19e-31 | 126 |
| Msa1005610 | AT4G31920.1 | 33.333 | 204 | 135 | 1 | 1 | 204 | 44 | 246 | 2.83e-31 | 127 |
| Msa1005610 | AT2G01760.2 | 36.792 | 212 | 110 | 5 | 9 | 197 | 74 | 284 | 4.26e-31 | 125 |
| Msa1005610 | AT2G27070.1 | 33.846 | 260 | 126 | 7 | 1 | 217 | 45 | 301 | 5.48e-30 | 124 |
| Msa1005610 | AT5G58080.1 | 33.953 | 215 | 115 | 5 | 5 | 201 | 49 | 254 | 1.65e-29 | 123 |
| Msa1005610 | AT1G67710.1 | 34.934 | 229 | 127 | 6 | 1 | 208 | 38 | 265 | 5.39e-29 | 120 |
| Msa1005610 | AT2G01760.3 | 35.938 | 192 | 99 | 5 | 29 | 197 | 1 | 191 | 4.68e-26 | 108 |
| Msa1005610 | AT3G62670.1 | 35.385 | 195 | 116 | 5 | 9 | 193 | 74 | 268 | 1.19e-25 | 109 |
| Msa1005610 | AT1G67710.2 | 35.354 | 198 | 106 | 6 | 32 | 208 | 4 | 200 | 6.73e-24 | 105 |
| Msa1005610 | AT1G49190.1 | 31.628 | 215 | 123 | 5 | 5 | 198 | 65 | 276 | 1.22e-22 | 100 |
| Msa1005610 | AT5G49240.1 | 31.343 | 201 | 114 | 4 | 9 | 190 | 77 | 272 | 2.59e-22 | 97.8 |
| Msa1005610 | AT1G67710.3 | 35.912 | 181 | 94 | 6 | 49 | 208 | 12 | 191 | 9.33e-20 | 92.4 |
| Msa1005610 | AT5G59570.2 | 37.719 | 114 | 59 | 3 | 98 | 200 | 88 | 200 | 5.88e-17 | 82.0 |
| Msa1005610 | AT5G59570.1 | 37.719 | 114 | 59 | 3 | 98 | 200 | 88 | 200 | 5.88e-17 | 82.0 |
| Msa1005610 | AT3G10760.1 | 47.222 | 72 | 37 | 1 | 129 | 200 | 94 | 164 | 7.48e-17 | 82.4 |
| Msa1005610 | AT5G05090.1 | 48.571 | 70 | 35 | 1 | 128 | 197 | 69 | 137 | 1.65e-16 | 80.1 |
| Msa1005610 | AT2G40970.1 | 50.000 | 70 | 34 | 1 | 131 | 200 | 96 | 164 | 1.76e-16 | 79.7 |
| Msa1005610 | AT3G46640.2 | 48.611 | 72 | 34 | 2 | 131 | 200 | 133 | 203 | 2.59e-16 | 80.5 |
| Msa1005610 | AT3G46640.1 | 48.611 | 72 | 34 | 2 | 131 | 200 | 133 | 203 | 2.59e-16 | 80.5 |
| Msa1005610 | AT3G46640.3 | 48.611 | 72 | 34 | 2 | 131 | 200 | 133 | 203 | 3.97e-16 | 80.1 |
| Msa1005610 | AT5G44190.1 | 61.290 | 62 | 21 | 2 | 132 | 193 | 141 | 199 | 5.89e-15 | 77.4 |
| Msa1005610 | AT5G44190.2 | 61.290 | 62 | 21 | 2 | 132 | 193 | 141 | 199 | 6.02e-15 | 77.0 |
| Msa1005610 | AT2G20570.1 | 58.182 | 55 | 22 | 1 | 139 | 193 | 152 | 205 | 1.24e-12 | 70.5 |
| Msa1005610 | AT2G20570.2 | 59.615 | 52 | 20 | 1 | 142 | 193 | 171 | 221 | 5.83e-12 | 68.2 |
| Msa1005610 | AT4G18020.8 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.00e-11 | 67.8 |
| Msa1005610 | AT4G18020.11 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.00e-11 | 67.8 |
| Msa1005610 | AT4G18020.4 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.00e-11 | 67.8 |
| Msa1005610 | AT4G18020.5 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.00e-11 | 67.8 |
| Msa1005610 | AT4G18020.10 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.00e-11 | 67.8 |
| Msa1005610 | AT4G18020.7 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.00e-11 | 67.8 |
| Msa1005610 | AT4G18020.3 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.11e-11 | 67.8 |
| Msa1005610 | AT4G18020.2 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.11e-11 | 67.8 |
| Msa1005610 | AT4G18020.1 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.11e-11 | 67.8 |
| Msa1005610 | AT4G18020.6 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.11e-11 | 67.8 |
| Msa1005610 | AT4G18020.9 | 44.737 | 76 | 38 | 2 | 140 | 215 | 296 | 367 | 1.11e-11 | 67.8 |
| Msa1005610 | AT1G49190.2 | 33.708 | 89 | 59 | 0 | 5 | 93 | 65 | 153 | 4.46e-11 | 65.9 |
Find 84 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATTCTATTTCTGATATT+AGG | 0.142363 | 7_1:+3386892 | Msa1005610:CDS |
| CTGAAGACTTCAAAGATATT+TGG | 0.172247 | 7_1:+3385456 | Msa1005610:CDS |
| GTAAGAAACAATAACCATTT+TGG | 0.206140 | 7_1:+3386693 | Msa1005610:CDS |
| AAATTCTAACTTTGGATCTT+TGG | 0.211667 | 7_1:+3386632 | Msa1005610:CDS |
| GAATTATGAATAATGGATTA+AGG | 0.219129 | 7_1:+3386754 | Msa1005610:CDS |
| CAATTCCCTTACATACAAAA+AGG | 0.229371 | 7_1:+3386336 | Msa1005610:CDS |
| ACTTACTTTCCAATCCAATT+TGG | 0.246719 | 7_1:-3385694 | None:intergenic |
| CTGCTTGCTATTAACCAAAT+TGG | 0.262572 | 7_1:+3385680 | Msa1005610:CDS |
| GGATTCTATTTCTGATATTA+GGG | 0.272190 | 7_1:+3386893 | Msa1005610:CDS |
| GGAATTGGAATTATGAATAA+TGG | 0.274410 | 7_1:+3386747 | Msa1005610:CDS |
| AACTTGTGAGGCATAATATT+AGG | 0.274422 | 7_1:-3386660 | None:intergenic |
| AAATGTAGCAAGTCACTTAC+AGG | 0.277878 | 7_1:+3385900 | Msa1005610:CDS |
| ATGACACACTTACTTATTAT+AGG | 0.282733 | 7_1:-3384558 | None:intergenic |
| CAAAGCATGACGAGCATCTT+TGG | 0.288698 | 7_1:-3384424 | None:intergenic |
| TTTGGAATTCAGCTGAATAA+TGG | 0.297513 | 7_1:+3386711 | Msa1005610:CDS |
| CAACATGATGCGTTGGAAAT+TGG | 0.299414 | 7_1:-3386311 | None:intergenic |
| ACTTACTTATTATAGGTATC+TGG | 0.300133 | 7_1:-3384551 | None:intergenic |
| TATTCAGCTGAATTCCAAAA+TGG | 0.301790 | 7_1:-3386707 | None:intergenic |
| TTAAGAAACAAAAGCTTGTT+TGG | 0.309580 | 7_1:+3385633 | Msa1005610:CDS |
| TTGAAGTCTTCAGCACAAAA+AGG | 0.324880 | 7_1:-3385446 | None:intergenic |
| ATCTCAAAGACACTAGAAAA+TGG | 0.331289 | 7_1:+3385401 | Msa1005610:CDS |
| TTTACTCAACATGACTTAGA+TGG | 0.332469 | 7_1:+3386984 | Msa1005610:CDS |
| TTCAAGATTGGGAACAAGTT+AGG | 0.352069 | 7_1:+3386367 | Msa1005610:CDS |
| AGATATTTGGAAATATGTTA+TGG | 0.355548 | 7_1:+3385469 | Msa1005610:CDS |
| GGCTGTTCCAAAGAAAATTC+TGG | 0.359742 | 7_1:+3385843 | Msa1005610:CDS |
| AATTATGAATAATGGATTAA+GGG | 0.361569 | 7_1:+3386755 | Msa1005610:CDS |
| GGAGCAGAAATGGTTGGAAT+TGG | 0.369244 | 7_1:+3386732 | Msa1005610:CDS |
| TTTCATCTGGTGGGAATCAA+TGG | 0.369590 | 7_1:+3384495 | Msa1005610:CDS |
| AAGGGTTGGCTCTTCAAGAT+TGG | 0.372343 | 7_1:+3386355 | Msa1005610:CDS |
| ACTTGTGAGGCATAATATTA+GGG | 0.374491 | 7_1:-3386659 | None:intergenic |
| TGCTATTAACCAAATTGGAT+TGG | 0.390260 | 7_1:+3385685 | Msa1005610:CDS |
| CGTTATTGCAGAATTTCATC+TGG | 0.397811 | 7_1:+3384482 | Msa1005610:CDS |
| GGCCATGGTCAAAACAGGTT+TGG | 0.410656 | 7_1:+3386435 | Msa1005610:CDS |
| TAGAGGAGAACAAGGATTGA+TGG | 0.421210 | 7_1:+3386536 | Msa1005610:CDS |
| CTACTTTCACTAATGAGGTT+GGG | 0.423849 | 7_1:+3387042 | Msa1005610:CDS |
| GCTGAATAATGGAGCAGAAA+TGG | 0.428747 | 7_1:+3386722 | Msa1005610:CDS |
| CTGCGAGATGTTGAAGAGAA+AGG | 0.429447 | 7_1:+3386108 | Msa1005610:CDS |
| CAGTGATGTCTGAAGACAAT+AGG | 0.437039 | 7_1:+3385369 | Msa1005610:intron |
| AGGGTTGGCTCTTCAAGATT+GGG | 0.442206 | 7_1:+3386356 | Msa1005610:CDS |
| GCGTTGGAAATTGGTTGAAA+TGG | 0.443167 | 7_1:-3386302 | None:intergenic |
| ATAATGGCCATGGTCAAAAC+AGG | 0.444362 | 7_1:+3386430 | Msa1005610:CDS |
| CTTCTTCAAGTTTGTTAACA+AGG | 0.448083 | 7_1:+3386565 | Msa1005610:CDS |
| ATCACTCTTCAAGAAAATGA+AGG | 0.453796 | 7_1:+3384447 | Msa1005610:CDS |
| GCTACTTTCACTAATGAGGT+TGG | 0.473474 | 7_1:+3387041 | Msa1005610:CDS |
| GGTTCAAGGAGGGTTTGCTC+TGG | 0.484414 | 7_1:+3386872 | Msa1005610:CDS |
| GCAGGATCAGTTAATAATGA+AGG | 0.491730 | 7_1:+3386939 | Msa1005610:CDS |
| TTCATCTGGTGGGAATCAAT+GGG | 0.491841 | 7_1:+3384496 | Msa1005610:CDS |
| AGTGTCTTTGAGATTAAACT+TGG | 0.491853 | 7_1:-3385392 | None:intergenic |
| GTTGAAGAGAAAGGAATGGT+TGG | 0.491902 | 7_1:+3386117 | Msa1005610:CDS |
| TCTGATATTAGGGGAGGGAG+TGG | 0.493957 | 7_1:+3386903 | Msa1005610:CDS |
| CTTGAATATCTCTCTCTCCT+TGG | 0.495918 | 7_1:-3385607 | None:intergenic |
| GATTGGGAACAAGTTAGGCT+TGG | 0.497594 | 7_1:+3386372 | Msa1005610:CDS |
| TAGTGCTACTTTCACTAATG+AGG | 0.497764 | 7_1:+3387037 | Msa1005610:CDS |
| CTGCAATAACGAGATCAAAC+AGG | 0.499260 | 7_1:-3384470 | None:intergenic |
| TAGGCTTGGAAAATCAAAGT+TGG | 0.508790 | 7_1:+3386386 | Msa1005610:CDS |
| ATTTCTCTGATCACAGAACT+TGG | 0.510262 | 7_1:-3386186 | None:intergenic |
| GATGTTGTTGCGGAGAATAA+TGG | 0.517615 | 7_1:+3386414 | Msa1005610:CDS |
| GACATTAGAAACCAGAATCA+TGG | 0.525782 | 7_1:+3386603 | Msa1005610:CDS |
| ATTGCAGAATTTCATCTGGT+GGG | 0.533922 | 7_1:+3384486 | Msa1005610:CDS |
| TTCTTCAAGTTTGTTAACAA+GGG | 0.536372 | 7_1:+3386566 | Msa1005610:CDS |
| AGTGGAAGAAAGTTTCTAGC+AGG | 0.545083 | 7_1:+3386921 | Msa1005610:CDS |
| AGCTAGTGGAAGCACCATCA+TGG | 0.545478 | 7_1:-3386503 | None:intergenic |
| GACTTAGATGGAAATGTTGT+GGG | 0.546117 | 7_1:+3386996 | Msa1005610:CDS |
| TATTGCAGAATTTCATCTGG+TGG | 0.554479 | 7_1:+3384485 | Msa1005610:CDS |
| AATAATGGAGCAGAAATGGT+TGG | 0.555811 | 7_1:+3386726 | Msa1005610:CDS |
| CTATTTCTGATATTAGGGGA+GGG | 0.562145 | 7_1:+3386898 | Msa1005610:CDS |
| TCTATATGATGAAAAGCTAG+TGG | 0.562508 | 7_1:-3386517 | None:intergenic |
| AATTTGGTTTGGTTCAAGGA+GGG | 0.563542 | 7_1:+3386862 | Msa1005610:CDS |
| GAAGAGAAAGGAATGGTTGG+AGG | 0.566539 | 7_1:+3386120 | Msa1005610:CDS |
| AATTGGCCAACATGATGCGT+TGG | 0.583725 | 7_1:-3386318 | None:intergenic |
| TCTATTTCTGATATTAGGGG+AGG | 0.587568 | 7_1:+3386897 | Msa1005610:CDS |
| AAATTTGGTTTGGTTCAAGG+AGG | 0.591953 | 7_1:+3386861 | Msa1005610:CDS |
| TCATCATATAGAGGAGAACA+AGG | 0.594028 | 7_1:+3386528 | Msa1005610:CDS |
| TGACTTAGATGGAAATGTTG+TGG | 0.597242 | 7_1:+3386995 | Msa1005610:CDS |
| GATTCTATTTCTGATATTAG+GGG | 0.603781 | 7_1:+3386894 | Msa1005610:CDS |
| AGAAAGAGTAAAGAATGCCA+AGG | 0.604346 | 7_1:+3385590 | Msa1005610:CDS |
| CAATTTCCAACGCATCATGT+TGG | 0.607673 | 7_1:+3386312 | Msa1005610:CDS |
| GTTGGAAGCTGATGTTGTTG+CGG | 0.645784 | 7_1:+3386404 | Msa1005610:CDS |
| AAAGTCTTCATACCTTGTTG+CGG | 0.645913 | 7_1:-3387121 | None:intergenic |
| TGACAAGAATCTCCGCAACA+AGG | 0.646714 | 7_1:+3387109 | Msa1005610:CDS |
| AAACTGGCATATCAAACAAG+AGG | 0.659644 | 7_1:+3386261 | Msa1005610:CDS |
| AGATGTTGAAGAGAAAGGAA+TGG | 0.660542 | 7_1:+3386113 | Msa1005610:CDS |
| ACATCAGAAGTAAACTTGTG+AGG | 0.669577 | 7_1:-3386672 | None:intergenic |
| GTTGCGGAGAATAATGGCCA+TGG | 0.690083 | 7_1:+3386420 | Msa1005610:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAATATTTTTCTTATGAA+AGG | + | chr7_1:3385725-3385744 | Msa1005610:intron | 10.0% |
| !!! | TATAATATTTTTGTGTCATT+TGG | + | chr7_1:3384886-3384905 | Msa1005610:intron | 15.0% |
| !! | TATAAACAACTTTAATAAGT+TGG | + | chr7_1:3385127-3385146 | Msa1005610:intron | 15.0% |
| !!! | AATTATGAATAATGGATTAA+GGG | + | chr7_1:3386755-3386774 | Msa1005610:CDS | 15.0% |
| !!! | AACTGGATTTCTAAAATAAA+TGG | - | chr7_1:3384733-3384752 | None:intergenic | 20.0% |
| !!! | TATTTTTCATGAAAATCGTA+AGG | - | chr7_1:3384770-3384789 | None:intergenic | 20.0% |
| !!! | TCATTAACTCTTTAAAAATG+TGG | + | chr7_1:3384845-3384864 | Msa1005610:intron | 20.0% |
| !!! | TTAAAAATGTGGTCAAATTT+TGG | + | chr7_1:3384856-3384875 | Msa1005610:intron | 20.0% |
| !! | TAATAAAGTTTAATGTAACG+TGG | + | chr7_1:3385088-3385107 | Msa1005610:intron | 20.0% |
| !! | AGATATTTGGAAATATGTTA+TGG | + | chr7_1:3385469-3385488 | Msa1005610:CDS | 20.0% |
| !!! | TTAACAAGGGATTTTAAATT+TGG | + | chr7_1:3386579-3386598 | Msa1005610:CDS | 20.0% |
| !!! | GAATTATGAATAATGGATTA+AGG | + | chr7_1:3386754-3386773 | Msa1005610:CDS | 20.0% |
| !!! | TCTTTTGTGAATGAAAAATT+TGG | + | chr7_1:3386846-3386865 | Msa1005610:CDS | 20.0% |
| !!! | CAACAATTTTGATTACTTAA+TGG | + | chr7_1:3387082-3387101 | Msa1005610:CDS | 20.0% |
| ! | ACTTACTTATTATAGGTATC+TGG | - | chr7_1:3384554-3384573 | None:intergenic | 25.0% |
| ! | ATGACACACTTACTTATTAT+AGG | - | chr7_1:3384561-3384580 | None:intergenic | 25.0% |
| ! | AACAAGTAAGCATTAAAAAC+TGG | - | chr7_1:3384638-3384657 | None:intergenic | 25.0% |
| ! | TTATTATGCAAACATGCTTA+AGG | + | chr7_1:3384657-3384676 | Msa1005610:intron | 25.0% |
| ! | AGGTTAAGATAATAAAGAAC+TGG | - | chr7_1:3384750-3384769 | None:intergenic | 25.0% |
| !! | TGTCATTTGGATAGATAATT+TGG | + | chr7_1:3384899-3384918 | Msa1005610:intron | 25.0% |
| !!! | AGACATTATTTGTGTCATTT+TGG | + | chr7_1:3384999-3385018 | Msa1005610:intron | 25.0% |
| !! | TTAAGAAACAAAAGCTTGTT+TGG | + | chr7_1:3385633-3385652 | Msa1005610:CDS | 25.0% |
| !! | TTATATTTTCCCTAATTCAG+AGG | + | chr7_1:3385822-3385841 | Msa1005610:intron | 25.0% |
| !! | TTTTGCAAATATTGAAGTGT+TGG | - | chr7_1:3386243-3386262 | None:intergenic | 25.0% |
| ! | ACTTCAATATTTGCAAAAAC+TGG | + | chr7_1:3386245-3386264 | Msa1005610:CDS | 25.0% |
| ! | AATTCCCTTACATACAAAAA+GGG | + | chr7_1:3386337-3386356 | Msa1005610:CDS | 25.0% |
| !! | TTTTTGCTTGAAAAAATGGA+AGG | - | chr7_1:3386476-3386495 | None:intergenic | 25.0% |
| !!! | GAGTTTTTTGCTTGAAAAAA+TGG | - | chr7_1:3386480-3386499 | None:intergenic | 25.0% |
| !!! | TTCTTCAAGTTTGTTAACAA+GGG | + | chr7_1:3386566-3386585 | Msa1005610:CDS | 25.0% |
| !! | AATTTTTAACACCATGATTC+TGG | - | chr7_1:3386617-3386636 | None:intergenic | 25.0% |
| ! | GGTGTTAAAAATTCTAACTT+TGG | + | chr7_1:3386624-3386643 | Msa1005610:CDS | 25.0% |
| ! | AAATTCTAACTTTGGATCTT+TGG | + | chr7_1:3386632-3386651 | Msa1005610:CDS | 25.0% |
| !! | GTAAGAAACAATAACCATTT+TGG | + | chr7_1:3386693-3386712 | Msa1005610:CDS | 25.0% |
| ! | GGAATTGGAATTATGAATAA+TGG | + | chr7_1:3386747-3386766 | Msa1005610:CDS | 25.0% |
| ! | TGTGAATGAAAAATTTGGTT+TGG | + | chr7_1:3386851-3386870 | Msa1005610:CDS | 25.0% |
| !!! | TGGATTCTATTTCTGATATT+AGG | + | chr7_1:3386892-3386911 | Msa1005610:CDS | 25.0% |
| !!! | GGATTCTATTTCTGATATTA+GGG | + | chr7_1:3386893-3386912 | Msa1005610:CDS | 25.0% |
| !!! | GATTCTATTTCTGATATTAG+GGG | + | chr7_1:3386894-3386913 | Msa1005610:CDS | 25.0% |
| ATCACTCTTCAAGAAAATGA+AGG | + | chr7_1:3384447-3384466 | Msa1005610:CDS | 30.0% | |
| TTTAGTGCACCTATCAATTT+AGG | - | chr7_1:3384692-3384711 | None:intergenic | 30.0% | |
| AGTCAAACTCAAAATTACTG+AGG | - | chr7_1:3384793-3384812 | None:intergenic | 30.0% | |
| AAGAAGCTTAAAGAACTGTA+TGG | - | chr7_1:3385238-3385257 | None:intergenic | 30.0% | |
| TGCATAAGAACTAAAGTAGT+GGG | - | chr7_1:3385314-3385333 | None:intergenic | 30.0% | |
| ATGCATAAGAACTAAAGTAG+TGG | - | chr7_1:3385315-3385334 | None:intergenic | 30.0% | |
| !! | AGTGTCTTTGAGATTAAACT+TGG | - | chr7_1:3385395-3385414 | None:intergenic | 30.0% |
| ATCTCAAAGACACTAGAAAA+TGG | + | chr7_1:3385401-3385420 | Msa1005610:CDS | 30.0% | |
| CTGAAGACTTCAAAGATATT+TGG | + | chr7_1:3385456-3385475 | Msa1005610:CDS | 30.0% | |
| ! | CAATGCTTCTTTTTGTTCTT+AGG | - | chr7_1:3385566-3385585 | None:intergenic | 30.0% |
| ! | AGAAACATTTTGTGCAGATA+AGG | - | chr7_1:3385662-3385681 | None:intergenic | 30.0% |
| TGCTATTAACCAAATTGGAT+TGG | + | chr7_1:3385685-3385704 | Msa1005610:CDS | 30.0% | |
| ACTTACTTTCCAATCCAATT+TGG | - | chr7_1:3385697-3385716 | None:intergenic | 30.0% | |
| ! | ATGATCTCCAGAATTTTCTT+TGG | - | chr7_1:3385853-3385872 | None:intergenic | 30.0% |
| AAGACAACGACAAAAATTAG+TGG | - | chr7_1:3386012-3386031 | None:intergenic | 30.0% | |
| ! | ATAAGATCAAGTTTTGCATC+AGG | + | chr7_1:3386159-3386178 | Msa1005610:CDS | 30.0% |
| TCAGAGAAATCGAAGAAAAA+AGG | + | chr7_1:3386199-3386218 | Msa1005610:CDS | 30.0% | |
| ! | CTTTTTGTATGTAAGGGAAT+TGG | - | chr7_1:3386338-3386357 | None:intergenic | 30.0% |
| CAATTCCCTTACATACAAAA+AGG | + | chr7_1:3386336-3386355 | Msa1005610:CDS | 30.0% | |
| TCTATATGATGAAAAGCTAG+TGG | - | chr7_1:3386520-3386539 | None:intergenic | 30.0% | |
| !!! | ACTAGCTTTTCATCATATAG+AGG | + | chr7_1:3386519-3386538 | Msa1005610:CDS | 30.0% |
| !! | CTTCTTCAAGTTTGTTAACA+AGG | + | chr7_1:3386565-3386584 | Msa1005610:CDS | 30.0% |
| ACTTGTGAGGCATAATATTA+GGG | - | chr7_1:3386662-3386681 | None:intergenic | 30.0% | |
| AACTTGTGAGGCATAATATT+AGG | - | chr7_1:3386663-3386682 | None:intergenic | 30.0% | |
| TATTCAGCTGAATTCCAAAA+TGG | - | chr7_1:3386710-3386729 | None:intergenic | 30.0% | |
| ! | TTTGGAATTCAGCTGAATAA+TGG | + | chr7_1:3386711-3386730 | Msa1005610:CDS | 30.0% |
| ! | ATAGTTTTTGCTTCAACGAA+AGG | + | chr7_1:3386796-3386815 | Msa1005610:CDS | 30.0% |
| ! | TAGTTTTTGCTTCAACGAAA+GGG | + | chr7_1:3386797-3386816 | Msa1005610:CDS | 30.0% |
| !! | GAAAAATTTGGTTTGGTTCA+AGG | + | chr7_1:3386858-3386877 | Msa1005610:CDS | 30.0% |
| TTTACTCAACATGACTTAGA+TGG | + | chr7_1:3386984-3387003 | Msa1005610:CDS | 30.0% | |
| CGTTATTGCAGAATTTCATC+TGG | + | chr7_1:3384482-3384501 | Msa1005610:CDS | 35.0% | |
| TATTGCAGAATTTCATCTGG+TGG | + | chr7_1:3384485-3384504 | Msa1005610:CDS | 35.0% | |
| ATTGCAGAATTTCATCTGGT+GGG | + | chr7_1:3384486-3384505 | Msa1005610:CDS | 35.0% | |
| !! | TTGTTTTTCTTTGCATGCAC+AGG | + | chr7_1:3384607-3384626 | Msa1005610:intron | 35.0% |
| ! | TGTTTTTCTTTGCATGCACA+GGG | + | chr7_1:3384608-3384627 | Msa1005610:intron | 35.0% |
| TGCAAACATGCTTAAGGATT+TGG | + | chr7_1:3384663-3384682 | Msa1005610:intron | 35.0% | |
| GCAAACATGCTTAAGGATTT+GGG | + | chr7_1:3384664-3384683 | Msa1005610:intron | 35.0% | |
| ATTTGGGCTCCTAAATTGAT+AGG | + | chr7_1:3384680-3384699 | Msa1005610:intron | 35.0% | |
| TTGAAGTCTTCAGCACAAAA+AGG | - | chr7_1:3385449-3385468 | None:intergenic | 35.0% | |
| AGAAAGAGTAAAGAATGCCA+AGG | + | chr7_1:3385590-3385609 | Msa1005610:CDS | 35.0% | |
| !! | CTGCTTGCTATTAACCAAAT+TGG | + | chr7_1:3385680-3385699 | Msa1005610:CDS | 35.0% |
| AAATGTAGCAAGTCACTTAC+AGG | + | chr7_1:3385900-3385919 | Msa1005610:CDS | 35.0% | |
| AGATGTTGAAGAGAAAGGAA+TGG | + | chr7_1:3386113-3386132 | Msa1005610:CDS | 35.0% | |
| ATTTCTCTGATCACAGAACT+TGG | - | chr7_1:3386189-3386208 | None:intergenic | 35.0% | |
| AAACTGGCATATCAAACAAG+AGG | + | chr7_1:3386261-3386280 | Msa1005610:CDS | 35.0% | |
| ! | CCAACCCTTTTTGTATGTAA+GGG | - | chr7_1:3386344-3386363 | None:intergenic | 35.0% |
| TTCAAGATTGGGAACAAGTT+AGG | + | chr7_1:3386367-3386386 | Msa1005610:CDS | 35.0% | |
| TAGGCTTGGAAAATCAAAGT+TGG | + | chr7_1:3386386-3386405 | Msa1005610:CDS | 35.0% | |
| GCAAAAAACTCATTCCATGA+TGG | + | chr7_1:3386489-3386508 | Msa1005610:CDS | 35.0% | |
| TCATCATATAGAGGAGAACA+AGG | + | chr7_1:3386528-3386547 | Msa1005610:CDS | 35.0% | |
| GACATTAGAAACCAGAATCA+TGG | + | chr7_1:3386603-3386622 | Msa1005610:CDS | 35.0% | |
| ACATCAGAAGTAAACTTGTG+AGG | - | chr7_1:3386675-3386694 | None:intergenic | 35.0% | |
| ! | AATAATGGAGCAGAAATGGT+TGG | + | chr7_1:3386726-3386745 | Msa1005610:CDS | 35.0% |
| ! | AGTTTTTGCTTCAACGAAAG+GGG | + | chr7_1:3386798-3386817 | Msa1005610:CDS | 35.0% |
| !! | AAATTTGGTTTGGTTCAAGG+AGG | + | chr7_1:3386861-3386880 | Msa1005610:CDS | 35.0% |
| !! | AATTTGGTTTGGTTCAAGGA+GGG | + | chr7_1:3386862-3386881 | Msa1005610:CDS | 35.0% |
| !! | TCTATTTCTGATATTAGGGG+AGG | + | chr7_1:3386897-3386916 | Msa1005610:CDS | 35.0% |
| !! | CTATTTCTGATATTAGGGGA+GGG | + | chr7_1:3386898-3386917 | Msa1005610:CDS | 35.0% |
| GCAGGATCAGTTAATAATGA+AGG | + | chr7_1:3386939-3386958 | Msa1005610:CDS | 35.0% | |
| TGACTTAGATGGAAATGTTG+TGG | + | chr7_1:3386995-3387014 | Msa1005610:CDS | 35.0% | |
| GACTTAGATGGAAATGTTGT+GGG | + | chr7_1:3386996-3387015 | Msa1005610:CDS | 35.0% | |
| !! | TAGTGCTACTTTCACTAATG+AGG | + | chr7_1:3387037-3387056 | Msa1005610:CDS | 35.0% |
| CTACTTTCACTAATGAGGTT+GGG | + | chr7_1:3387042-3387061 | Msa1005610:CDS | 35.0% | |
| CTGCAATAACGAGATCAAAC+AGG | - | chr7_1:3384473-3384492 | None:intergenic | 40.0% | |
| TTTCATCTGGTGGGAATCAA+TGG | + | chr7_1:3384495-3384514 | Msa1005610:CDS | 40.0% | |
| TTCATCTGGTGGGAATCAAT+GGG | + | chr7_1:3384496-3384515 | Msa1005610:CDS | 40.0% | |
| CAGTGATGTCTGAAGACAAT+AGG | + | chr7_1:3385369-3385388 | Msa1005610:intron | 40.0% | |
| CTTGAATATCTCTCTCTCCT+TGG | - | chr7_1:3385610-3385629 | None:intergenic | 40.0% | |
| TTGGAACAGCCTCTGAATTA+GGG | - | chr7_1:3385834-3385853 | None:intergenic | 40.0% | |
| ! | TTTGGAACAGCCTCTGAATT+AGG | - | chr7_1:3385835-3385854 | None:intergenic | 40.0% |
| GGCTGTTCCAAAGAAAATTC+TGG | + | chr7_1:3385843-3385862 | Msa1005610:CDS | 40.0% | |
| ! | ACATTTTCTCTGGTCAGGTT+TGG | - | chr7_1:3385886-3385905 | None:intergenic | 40.0% |
| ! | TTGCTACATTTTCTCTGGTC+AGG | - | chr7_1:3385891-3385910 | None:intergenic | 40.0% |
| !!! | GTGACTTGCTACATTTTCTC+TGG | - | chr7_1:3385896-3385915 | None:intergenic | 40.0% |
| GTTGAAGAGAAAGGAATGGT+TGG | + | chr7_1:3386117-3386136 | Msa1005610:CDS | 40.0% | |
| GCGTTGGAAATTGGTTGAAA+TGG | - | chr7_1:3386305-3386324 | None:intergenic | 40.0% | |
| CAACATGATGCGTTGGAAAT+TGG | - | chr7_1:3386314-3386333 | None:intergenic | 40.0% | |
| CAATTTCCAACGCATCATGT+TGG | + | chr7_1:3386312-3386331 | Msa1005610:CDS | 40.0% | |
| CCCTTACATACAAAAAGGGT+TGG | + | chr7_1:3386341-3386360 | Msa1005610:CDS | 40.0% | |
| ! | GCCAACCCTTTTTGTATGTA+AGG | - | chr7_1:3386345-3386364 | None:intergenic | 40.0% |
| GATGTTGTTGCGGAGAATAA+TGG | + | chr7_1:3386414-3386433 | Msa1005610:CDS | 40.0% | |
| ATAATGGCCATGGTCAAAAC+AGG | + | chr7_1:3386430-3386449 | Msa1005610:CDS | 40.0% | |
| !!! | TTCCAAACCTGTTTTGACCA+TGG | - | chr7_1:3386440-3386459 | None:intergenic | 40.0% |
| TAGAGGAGAACAAGGATTGA+TGG | + | chr7_1:3386536-3386555 | Msa1005610:CDS | 40.0% | |
| ! | GCTGAATAATGGAGCAGAAA+TGG | + | chr7_1:3386722-3386741 | Msa1005610:CDS | 40.0% |
| !! | AGTGGAAGAAAGTTTCTAGC+AGG | + | chr7_1:3386921-3386940 | Msa1005610:CDS | 40.0% |
| ! | GCTACTTTCACTAATGAGGT+TGG | + | chr7_1:3387041-3387060 | Msa1005610:CDS | 40.0% |
| CAAAGCATGACGAGCATCTT+TGG | - | chr7_1:3384427-3384446 | None:intergenic | 45.0% | |
| CTGCGAGATGTTGAAGAGAA+AGG | + | chr7_1:3386108-3386127 | Msa1005610:CDS | 45.0% | |
| GAAGAGAAAGGAATGGTTGG+AGG | + | chr7_1:3386120-3386139 | Msa1005610:CDS | 45.0% | |
| AATTGGCCAACATGATGCGT+TGG | - | chr7_1:3386321-3386340 | None:intergenic | 45.0% | |
| !! | AAGGGTTGGCTCTTCAAGAT+TGG | + | chr7_1:3386355-3386374 | Msa1005610:CDS | 45.0% |
| !! | AGGGTTGGCTCTTCAAGATT+GGG | + | chr7_1:3386356-3386375 | Msa1005610:CDS | 45.0% |
| GATTGGGAACAAGTTAGGCT+TGG | + | chr7_1:3386372-3386391 | Msa1005610:CDS | 45.0% | |
| GTTGGAAGCTGATGTTGTTG+CGG | + | chr7_1:3386404-3386423 | Msa1005610:CDS | 45.0% | |
| GGAGCAGAAATGGTTGGAAT+TGG | + | chr7_1:3386732-3386751 | Msa1005610:CDS | 45.0% | |
| TGACAAGAATCTCCGCAACA+AGG | + | chr7_1:3387109-3387128 | Msa1005610:CDS | 45.0% | |
| !!! | TATTTTGATAAATAATTTAT+AGG | + | chr7_1:3385032-3385051 | Msa1005610:intron | 5.0% |
| GTTGCGGAGAATAATGGCCA+TGG | + | chr7_1:3386420-3386439 | Msa1005610:CDS | 50.0% | |
| GGCCATGGTCAAAACAGGTT+TGG | + | chr7_1:3386435-3386454 | Msa1005610:CDS | 50.0% | |
| !! | AGCTAGTGGAAGCACCATCA+TGG | - | chr7_1:3386506-3386525 | None:intergenic | 50.0% |
| TCTGATATTAGGGGAGGGAG+TGG | + | chr7_1:3386903-3386922 | Msa1005610:CDS | 50.0% | |
| ! | GGTTCAAGGAGGGTTTGCTC+TGG | + | chr7_1:3386872-3386891 | Msa1005610:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 3384417 | 3387136 | 3384417 | ID=Msa1005610;Name=Msa1005610 |
| chr7_1 | mRNA | 3384417 | 3387136 | 3384417 | ID=Msa1005610-mRNA-1;Parent=Msa1005610;Name=Msa1005610-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|0.75|4|0|537 |
| chr7_1 | exon | 3384417 | 3384567 | 3384417 | ID=Msa1005610-mRNA-1:exon:1369;Parent=Msa1005610-mRNA-1 |
| chr7_1 | exon | 3385372 | 3385710 | 3385372 | ID=Msa1005610-mRNA-1:exon:1370;Parent=Msa1005610-mRNA-1 |
| chr7_1 | exon | 3385842 | 3385921 | 3385842 | ID=Msa1005610-mRNA-1:exon:1371;Parent=Msa1005610-mRNA-1 |
| chr7_1 | exon | 3386093 | 3387136 | 3386093 | ID=Msa1005610-mRNA-1:exon:1372;Parent=Msa1005610-mRNA-1 |
| chr7_1 | CDS | 3384417 | 3384567 | 3384417 | ID=Msa1005610-mRNA-1:cds;Parent=Msa1005610-mRNA-1 |
| chr7_1 | CDS | 3385372 | 3385710 | 3385372 | ID=Msa1005610-mRNA-1:cds;Parent=Msa1005610-mRNA-1 |
| chr7_1 | CDS | 3385842 | 3385921 | 3385842 | ID=Msa1005610-mRNA-1:cds;Parent=Msa1005610-mRNA-1 |
| chr7_1 | CDS | 3386093 | 3387136 | 3386093 | ID=Msa1005610-mRNA-1:cds;Parent=Msa1005610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
