Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007120 | A0A4P1RP25 | 69.231 | 39 | 12 | 0 | 2 | 40 | 21 | 59 | 5.27e-11 | 65.1 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1007120 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007120 | MtrunA17_Chr3g0113891 | 66.667 | 39 | 13 | 0 | 2 | 40 | 24 | 62 | 6.89e-14 | 63.2 |
| Msa1007120 | MtrunA17_Chr5g0437401 | 69.231 | 39 | 12 | 0 | 2 | 40 | 21 | 59 | 1.03e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAAGGTCCACAAGGTATT+TGG | 0.188295 | 7_1:-6519793 | Msa1007120:CDS |
| ACATAGCTTCGGTCATAAAC+TGG | 0.301911 | 7_1:+6519671 | None:intergenic |
| TTTGAAGTGTATAAAAGTAT+AGG | 0.322451 | 7_1:-6519745 | Msa1007120:CDS |
| TTGAAGTGTATAAAAGTATA+GGG | 0.335851 | 7_1:-6519744 | Msa1007120:CDS |
| GCAATATGTAAATATACAAT+TGG | 0.344878 | 7_1:+6519843 | None:intergenic |
| TCCAAATACCTTGTGGACCT+TGG | 0.461303 | 7_1:+6519792 | None:intergenic |
| CAATATGTAAATATACAATT+GGG | 0.492428 | 7_1:+6519844 | None:intergenic |
| ATCTCTTGCACTCATCAGAA+TGG | 0.506971 | 7_1:+6519818 | None:intergenic |
| CATCCTTTGCAACATAGCTT+CGG | 0.508683 | 7_1:+6519660 | None:intergenic |
| CATAGCTTCGGTCATAAACT+GGG | 0.549032 | 7_1:+6519672 | None:intergenic |
| CTAGTCATGTTAAAGTTCCA+CGG | 0.557872 | 7_1:+6519629 | None:intergenic |
| TGTTTCCTCCAAATACCTTG+TGG | 0.575556 | 7_1:+6519785 | None:intergenic |
| AAGGTCCACAAGGTATTTGG+AGG | 0.579293 | 7_1:-6519790 | Msa1007120:CDS |
| TGACCGAAGCTATGTTGCAA+AGG | 0.589935 | 7_1:-6519663 | Msa1007120:CDS |
| GAGATTCACCAAGGTCCACA+AGG | 0.618512 | 7_1:-6519800 | Msa1007120:CDS |
| TGGCTTACGAGGAAAACACA+AGG | 0.691624 | 7_1:-6519703 | Msa1007120:CDS |
| CAAAGGATGACTGAAAACCG+TGG | 0.705802 | 7_1:-6519646 | Msa1007120:CDS |
| TGAGTGCAAGAGATTCACCA+AGG | 0.768339 | 7_1:-6519809 | Msa1007120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATATGTAAATATACAATT+GGG | + | chr7_1:6519929-6519948 | None:intergenic | 15.0% |
| !! | CTAAAATAAGATCATGAAAA+TGG | + | chr7_1:6519655-6519674 | None:intergenic | 20.0% |
| !!! | CATTTTCATGATCTTATTTT+AGG | - | chr7_1:6519653-6519672 | Msa1007120:CDS | 20.0% |
| !! | TTAATTAATGGTAAACAACT+TGG | + | chr7_1:6519760-6519779 | None:intergenic | 20.0% |
| !! | AAGTTGTTTACCATTAATTA+AGG | - | chr7_1:6519759-6519778 | Msa1007120:CDS | 20.0% |
| !! | GCAATATGTAAATATACAAT+TGG | + | chr7_1:6519930-6519949 | None:intergenic | 20.0% |
| !!! | TTTGAAGTGTATAAAAGTAT+AGG | - | chr7_1:6520025-6520044 | Msa1007120:intron | 20.0% |
| !!! | TTGAAGTGTATAAAAGTATA+GGG | - | chr7_1:6520026-6520045 | Msa1007120:intron | 20.0% |
| !!! | ATGTATCTAGCCTTAATTAA+TGG | + | chr7_1:6519772-6519791 | None:intergenic | 25.0% |
| !! | GAGCTTTTTAATAATTTCCA+AGG | + | chr7_1:6519869-6519888 | None:intergenic | 25.0% |
| AACAAAAGAACAAGGAACAA+GGG | + | chr7_1:6519715-6519734 | None:intergenic | 30.0% | |
| ! | GTTGTTTCAACAAAAGAACA+AGG | + | chr7_1:6519723-6519742 | None:intergenic | 30.0% |
| ATAATCGGACTCATCATAAA+TGG | + | chr7_1:6519828-6519847 | None:intergenic | 30.0% | |
| AGTCCTATAAGCATGATAAT+CGG | + | chr7_1:6519843-6519862 | None:intergenic | 30.0% | |
| ACAAAAGAACAAGGAACAAG+GGG | + | chr7_1:6519714-6519733 | None:intergenic | 35.0% | |
| CAACAAAAGAACAAGGAACA+AGG | + | chr7_1:6519716-6519735 | None:intergenic | 35.0% | |
| AGTCCGATTATCATGCTTAT+AGG | - | chr7_1:6519837-6519856 | Msa1007120:CDS | 35.0% | |
| ATGCTTATAGGACTGAACCT+TGG | - | chr7_1:6519849-6519868 | Msa1007120:CDS | 40.0% | |
| ATCTCTTGCACTCATCAGAA+TGG | + | chr7_1:6519955-6519974 | None:intergenic | 40.0% | |
| TGTTTCCTCCAAATACCTTG+TGG | + | chr7_1:6519988-6520007 | None:intergenic | 40.0% | |
| !!! | GGAAGACACAGTCATTTTTC+TGG | - | chr7_1:6520047-6520066 | Msa1007120:intron | 40.0% |
| !!! | AGTCATTTTTCTGGCTTACG+AGG | - | chr7_1:6520056-6520075 | Msa1007120:intron | 40.0% |
| CATAGCTTCGGTCATAAACT+GGG | + | chr7_1:6520101-6520120 | None:intergenic | 40.0% | |
| ACATAGCTTCGGTCATAAAC+TGG | + | chr7_1:6520102-6520121 | None:intergenic | 40.0% | |
| CATCCTTTGCAACATAGCTT+CGG | + | chr7_1:6520113-6520132 | None:intergenic | 40.0% | |
| TGAGTGCAAGAGATTCACCA+AGG | - | chr7_1:6519961-6519980 | Msa1007120:intron | 45.0% | |
| ! | ACCAAGGTCCACAAGGTATT+TGG | - | chr7_1:6519977-6519996 | Msa1007120:intron | 45.0% |
| TCCAAATACCTTGTGGACCT+TGG | + | chr7_1:6519981-6520000 | None:intergenic | 45.0% | |
| ! | AAGGTCCACAAGGTATTTGG+AGG | - | chr7_1:6519980-6519999 | Msa1007120:intron | 45.0% |
| TGGCTTACGAGGAAAACACA+AGG | - | chr7_1:6520067-6520086 | Msa1007120:intron | 45.0% | |
| TGACCGAAGCTATGTTGCAA+AGG | - | chr7_1:6520107-6520126 | Msa1007120:intron | 45.0% | |
| CAAAGGATGACTGAAAACCG+TGG | - | chr7_1:6520124-6520143 | Msa1007120:intron | 45.0% | |
| GAGATTCACCAAGGTCCACA+AGG | - | chr7_1:6519970-6519989 | Msa1007120:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 6519633 | 6520159 | 6519633 | ID=Msa1007120;Name=Msa1007120 |
| chr7_1 | mRNA | 6519633 | 6520159 | 6519633 | ID=Msa1007120-mRNA-1;Parent=Msa1007120;Name=Msa1007120-mRNA-1;_AED=0.45;_eAED=0.66;_QI=0|0|0|0.5|0|0|2|0|81 |
| chr7_1 | exon | 6520155 | 6520159 | 6520155 | ID=Msa1007120-mRNA-1:exon:1957;Parent=Msa1007120-mRNA-1 |
| chr7_1 | exon | 6519633 | 6519873 | 6519633 | ID=Msa1007120-mRNA-1:exon:1956;Parent=Msa1007120-mRNA-1 |
| chr7_1 | CDS | 6520155 | 6520159 | 6520155 | ID=Msa1007120-mRNA-1:cds;Parent=Msa1007120-mRNA-1 |
| chr7_1 | CDS | 6519633 | 6519873 | 6519633 | ID=Msa1007120-mRNA-1:cds;Parent=Msa1007120-mRNA-1 |
| Gene Sequence |
| Protein sequence |
