AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1008570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1008570 MtrunA17_Chr7g0218891 78.302 106 23 0 1 106 1 106 1.16e-46 152
Msa1008570 MtrunA17_Chr7g0218891 76.842 95 22 0 12 106 98 192 3.00e-42 140
Msa1008570 MtrunA17_Chr7g0218891 68.539 89 28 0 2 90 174 262 1.14e-34 121
Msa1008570 MtrunA17_Chr1g0205801 44.444 81 42 2 11 90 10 88 2.09e-12 59.7
Msa1008570 MtrunA17_Chr8g0364231 46.154 65 35 0 29 93 56 120 9.77e-12 61.2
Msa1008570 MtrunA17_Chr5g0415031 46.774 62 33 0 29 90 57 118 4.75e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1008570 AT1G24190.2 38.636 88 52 1 3 90 4 89 2.16e-11 60.8
Msa1008570 AT1G24190.3 38.636 88 52 1 3 90 4 89 2.20e-11 60.8
Msa1008570 AT1G24190.1 38.636 88 52 1 3 90 4 89 2.22e-11 60.8
Msa1008570 AT1G70060.2 37.500 88 53 1 3 90 4 89 4.71e-11 59.7
Msa1008570 AT1G70060.1 37.500 88 53 1 3 90 4 89 4.71e-11 59.7

Find 31 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGATTTATTCAAGTTAAC+AGG 0.306329 7_1:-9034962 Msa1008570:CDS
AGAACATTGTGACGTCAAAA+AGG 0.380889 7_1:-9035012 Msa1008570:CDS
ATCTGAGGAGGAACAATCTC+TGG 0.416325 7_1:-9034669 Msa1008570:CDS
GCTAACTTCAATTGTCTACT+TGG 0.423205 7_1:+9033969 None:intergenic
AAGGGATTATTTGAAGGTCA+TGG 0.456386 7_1:-9034749 Msa1008570:CDS
ATCCTTTGATGCATTGATGA+AGG 0.461664 7_1:-9034023 Msa1008570:CDS
TGCATCAAAGGATTGCCATC+TGG 0.478971 7_1:+9034033 None:intergenic
ACTTCAATTGTCTACTTGGA+AGG 0.486961 7_1:+9033973 None:intergenic
AGCTTGATTTCAAATCCATC+AGG 0.489510 7_1:+9034695 None:intergenic
AAATCTCAGTAAATTCTTGA+AGG 0.490603 7_1:-9034768 Msa1008570:CDS
TTCTTGAAGGGATTATTTGA+AGG 0.491097 7_1:-9034755 Msa1008570:CDS
ATTGTCTACTTGGAAGGGAA+TGG 0.494288 7_1:+9033979 None:intergenic
AACTTCATTGACGAACTCCT+TGG 0.507296 7_1:+9034989 None:intergenic
TTGAACTACTTCATGCCTGA+TGG 0.515459 7_1:-9034710 Msa1008570:CDS
AATCTCAGTAAATTCTTGAA+GGG 0.520509 7_1:-9034767 Msa1008570:CDS
AAGGGACAATATCTACCCTA+TGG 0.523510 7_1:-9034004 Msa1008570:CDS
TTGGAAGGGAATGGATCCAT+AGG 0.524135 7_1:+9033988 None:intergenic
CTTCAATTGTCTACTTGGAA+GGG 0.537149 7_1:+9033974 None:intergenic
ATTGCCATCTGGAGAGAGGA+TGG 0.548537 7_1:+9034044 None:intergenic
TCCTTTGATGCATTGATGAA+GGG 0.554862 7_1:-9034022 Msa1008570:CDS
TCCCTTCATCAATGCATCAA+AGG 0.562349 7_1:+9034021 None:intergenic
TGATGCATTACAAGAAACAA+AGG 0.583155 7_1:-9034908 Msa1008570:intron
TGAAATCAAGCTTCTATCTG+AGG 0.589635 7_1:-9034684 Msa1008570:CDS
GATTTATTCAAGTTAACAGG+AGG 0.594070 7_1:-9034959 Msa1008570:CDS
TGGAAGGGAATGGATCCATA+GGG 0.599599 7_1:+9033989 None:intergenic
TAAACCATCCTCTCTCCAGA+TGG 0.604721 7_1:-9034048 Msa1008570:CDS
GGGCAACCCGTGATAATGGA+AGG 0.616033 7_1:-9035044 None:intergenic
ATTTATTCAAGTTAACAGGA+GGG 0.617938 7_1:-9034958 Msa1008570:CDS
AAGGATTGCCATCTGGAGAG+AGG 0.624231 7_1:+9034040 None:intergenic
AATCAAGCTTCTATCTGAGG+AGG 0.631110 7_1:-9034681 Msa1008570:CDS
TGGTTTAGTCGAAAACGTTG+AGG 0.659672 7_1:+9034064 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTACAATAAAAAAAATAG+TGG + chr7_1:9034138-9034157 None:intergenic 15.0%
!! CATTACAATAAAAAAAATAG+TGG + chr7_1:9034138-9034157 None:intergenic 15.0%
!!! ATAGATTTATTCAAGTTAAC+AGG - chr7_1:9034043-9034062 Msa1008570:CDS 20.0%
!!! AACTAATTTATCTATCCTTT+TGG - chr7_1:9034190-9034209 Msa1008570:intron 20.0%
!!! ACATAGATACATTTATCTTT+TGG - chr7_1:9034721-9034740 Msa1008570:CDS 20.0%
!!! CATAGATACATTTATCTTTT+GGG - chr7_1:9034722-9034741 Msa1008570:CDS 20.0%
!!! ATAGATTTATTCAAGTTAAC+AGG - chr7_1:9034043-9034062 Msa1008570:CDS 20.0%
!!! AACTAATTTATCTATCCTTT+TGG - chr7_1:9034190-9034209 Msa1008570:intron 20.0%
!!! ACATAGATACATTTATCTTT+TGG - chr7_1:9034721-9034740 Msa1008570:CDS 20.0%
!!! CATAGATACATTTATCTTTT+GGG - chr7_1:9034722-9034741 Msa1008570:CDS 20.0%
!! ATTTATTCAAGTTAACAGGA+GGG - chr7_1:9034047-9034066 Msa1008570:CDS 25.0%
!! ATCCTTTTGGTATATTTTGT+AGG - chr7_1:9034203-9034222 Msa1008570:intron 25.0%
! ATCCTACAAAATATACCAAA+AGG + chr7_1:9034208-9034227 None:intergenic 25.0%
! AAATCTCAGTAAATTCTTGA+AGG - chr7_1:9034237-9034256 Msa1008570:intron 25.0%
! AATCTCAGTAAATTCTTGAA+GGG - chr7_1:9034238-9034257 Msa1008570:intron 25.0%
! TGGATATATTGAGTAAAACA+TGG - chr7_1:9034550-9034569 Msa1008570:intron 25.0%
! CAGAATCTATTTAATTGTTG+TGG + chr7_1:9034625-9034644 None:intergenic 25.0%
!! CTTTGGCTTATTAAAAAACA+AGG + chr7_1:9034648-9034667 None:intergenic 25.0%
!!! GTTTTTTAATAAGCCAAAGA+TGG - chr7_1:9034649-9034668 Msa1008570:CDS 25.0%
!! ATTTATTCAAGTTAACAGGA+GGG - chr7_1:9034047-9034066 Msa1008570:CDS 25.0%
!! ATCCTTTTGGTATATTTTGT+AGG - chr7_1:9034203-9034222 Msa1008570:intron 25.0%
! ATCCTACAAAATATACCAAA+AGG + chr7_1:9034208-9034227 None:intergenic 25.0%
! AAATCTCAGTAAATTCTTGA+AGG - chr7_1:9034237-9034256 Msa1008570:intron 25.0%
! AATCTCAGTAAATTCTTGAA+GGG - chr7_1:9034238-9034257 Msa1008570:intron 25.0%
! TGGATATATTGAGTAAAACA+TGG - chr7_1:9034550-9034569 Msa1008570:intron 25.0%
! CAGAATCTATTTAATTGTTG+TGG + chr7_1:9034625-9034644 None:intergenic 25.0%
!! CTTTGGCTTATTAAAAAACA+AGG + chr7_1:9034648-9034667 None:intergenic 25.0%
!!! GTTTTTTAATAAGCCAAAGA+TGG - chr7_1:9034649-9034668 Msa1008570:CDS 25.0%
! GATTTATTCAAGTTAACAGG+AGG - chr7_1:9034046-9034065 Msa1008570:CDS 30.0%
TGATGCATTACAAGAAACAA+AGG - chr7_1:9034097-9034116 Msa1008570:intron 30.0%
TTCTTGAAGGGATTATTTGA+AGG - chr7_1:9034250-9034269 Msa1008570:intron 30.0%
! GACAAATTCTTTCGCATTTT+TGG + chr7_1:9034374-9034393 None:intergenic 30.0%
CTATGTTATAGTGAACAAAG+TGG + chr7_1:9034707-9034726 None:intergenic 30.0%
!!! ACATTTATCTTTTGGGATCT+AGG - chr7_1:9034729-9034748 Msa1008570:CDS 30.0%
!! TCATATGCTTTTGAAGAATC+TGG + chr7_1:9034842-9034861 None:intergenic 30.0%
! GATTTATTCAAGTTAACAGG+AGG - chr7_1:9034046-9034065 Msa1008570:CDS 30.0%
TGATGCATTACAAGAAACAA+AGG - chr7_1:9034097-9034116 Msa1008570:intron 30.0%
TTCTTGAAGGGATTATTTGA+AGG - chr7_1:9034250-9034269 Msa1008570:intron 30.0%
! GACAAATTCTTTCGCATTTT+TGG + chr7_1:9034374-9034393 None:intergenic 30.0%
CTATGTTATAGTGAACAAAG+TGG + chr7_1:9034707-9034726 None:intergenic 30.0%
!!! ACATTTATCTTTTGGGATCT+AGG - chr7_1:9034729-9034748 Msa1008570:CDS 30.0%
!! TCATATGCTTTTGAAGAATC+TGG + chr7_1:9034842-9034861 None:intergenic 30.0%
AGAACATTGTGACGTCAAAA+AGG - chr7_1:9033993-9034012 Msa1008570:CDS 35.0%
AAGGGATTATTTGAAGGTCA+TGG - chr7_1:9034256-9034275 Msa1008570:intron 35.0%
AGCTTGATTTCAAATCCATC+AGG + chr7_1:9034313-9034332 None:intergenic 35.0%
TGAAATCAAGCTTCTATCTG+AGG - chr7_1:9034321-9034340 Msa1008570:intron 35.0%
GATTTAGTCGATATCGTTCT+TGG - chr7_1:9034530-9034549 Msa1008570:intron 35.0%
TCATGAGTTCTTATGGGAAA+TGG + chr7_1:9034777-9034796 None:intergenic 35.0%
TCCTAGTCATGAGTTCTTAT+GGG + chr7_1:9034783-9034802 None:intergenic 35.0%
ATCCTAGTCATGAGTTCTTA+TGG + chr7_1:9034784-9034803 None:intergenic 35.0%
ATAAGAACTCATGACTAGGA+TGG - chr7_1:9034783-9034802 Msa1008570:CDS 35.0%
ACCAGAAACATGTTTATGTG+AGG + chr7_1:9034811-9034830 None:intergenic 35.0%
!! TCACATAAACATGTTTCTGG+TGG - chr7_1:9034810-9034829 Msa1008570:intron 35.0%
!! CACATAAACATGTTTCTGGT+GGG - chr7_1:9034811-9034830 Msa1008570:intron 35.0%
AAAGTCCTTCGACAAGAATT+TGG + chr7_1:9034876-9034895 None:intergenic 35.0%
! ATTCTTGTCGAAGGACTTTT+TGG - chr7_1:9034877-9034896 Msa1008570:intron 35.0%
! TCAGCCATATGAAGTATCTT+TGG - chr7_1:9034914-9034933 Msa1008570:CDS 35.0%
CTGACCAAAGATACTTCATA+TGG + chr7_1:9034921-9034940 None:intergenic 35.0%
! ATCCTTTGATGCATTGATGA+AGG - chr7_1:9034982-9035001 Msa1008570:CDS 35.0%
! TCCTTTGATGCATTGATGAA+GGG - chr7_1:9034983-9035002 Msa1008570:CDS 35.0%
AGAACATTGTGACGTCAAAA+AGG - chr7_1:9033993-9034012 Msa1008570:CDS 35.0%
AAGGGATTATTTGAAGGTCA+TGG - chr7_1:9034256-9034275 Msa1008570:intron 35.0%
AGCTTGATTTCAAATCCATC+AGG + chr7_1:9034313-9034332 None:intergenic 35.0%
TGAAATCAAGCTTCTATCTG+AGG - chr7_1:9034321-9034340 Msa1008570:intron 35.0%
GATTTAGTCGATATCGTTCT+TGG - chr7_1:9034530-9034549 Msa1008570:intron 35.0%
TCATGAGTTCTTATGGGAAA+TGG + chr7_1:9034777-9034796 None:intergenic 35.0%
TCCTAGTCATGAGTTCTTAT+GGG + chr7_1:9034783-9034802 None:intergenic 35.0%
ATCCTAGTCATGAGTTCTTA+TGG + chr7_1:9034784-9034803 None:intergenic 35.0%
ATAAGAACTCATGACTAGGA+TGG - chr7_1:9034783-9034802 Msa1008570:CDS 35.0%
ACCAGAAACATGTTTATGTG+AGG + chr7_1:9034811-9034830 None:intergenic 35.0%
!! TCACATAAACATGTTTCTGG+TGG - chr7_1:9034810-9034829 Msa1008570:intron 35.0%
!! CACATAAACATGTTTCTGGT+GGG - chr7_1:9034811-9034830 Msa1008570:intron 35.0%
AAAGTCCTTCGACAAGAATT+TGG + chr7_1:9034876-9034895 None:intergenic 35.0%
! ATTCTTGTCGAAGGACTTTT+TGG - chr7_1:9034877-9034896 Msa1008570:intron 35.0%
! TCAGCCATATGAAGTATCTT+TGG - chr7_1:9034914-9034933 Msa1008570:CDS 35.0%
CTGACCAAAGATACTTCATA+TGG + chr7_1:9034921-9034940 None:intergenic 35.0%
! ATCCTTTGATGCATTGATGA+AGG - chr7_1:9034982-9035001 Msa1008570:CDS 35.0%
! TCCTTTGATGCATTGATGAA+GGG - chr7_1:9034983-9035002 Msa1008570:CDS 35.0%
AACTTCATTGACGAACTCCT+TGG + chr7_1:9034019-9034038 None:intergenic 40.0%
TTGAACTACTTCATGCCTGA+TGG - chr7_1:9034295-9034314 Msa1008570:intron 40.0%
AATCAAGCTTCTATCTGAGG+AGG - chr7_1:9034324-9034343 Msa1008570:intron 40.0%
AAGCCAAAGATGGCATTAAG+AGG - chr7_1:9034659-9034678 Msa1008570:CDS 40.0%
AGCCAAAGATGGCATTAAGA+GGG - chr7_1:9034660-9034679 Msa1008570:CDS 40.0%
TCCCATAAGAACTCATGACT+AGG - chr7_1:9034779-9034798 Msa1008570:CDS 40.0%
GCCTCACATAAACATGTTTC+TGG - chr7_1:9034807-9034826 Msa1008570:intron 40.0%
ATTCGCCAAATTCTTGTCGA+AGG - chr7_1:9034868-9034887 Msa1008570:intron 40.0%
!!! CGAAGGACTTTTTGGTTATG+TGG - chr7_1:9034885-9034904 Msa1008570:intron 40.0%
TGGTTTAGTCGAAAACGTTG+AGG + chr7_1:9034944-9034963 None:intergenic 40.0%
TCCCTTCATCAATGCATCAA+AGG + chr7_1:9034987-9035006 None:intergenic 40.0%
AAGGGACAATATCTACCCTA+TGG - chr7_1:9035001-9035020 Msa1008570:CDS 40.0%
ATTGTCTACTTGGAAGGGAA+TGG + chr7_1:9035029-9035048 None:intergenic 40.0%
AACTTCATTGACGAACTCCT+TGG + chr7_1:9034019-9034038 None:intergenic 40.0%
TTGAACTACTTCATGCCTGA+TGG - chr7_1:9034295-9034314 Msa1008570:intron 40.0%
AATCAAGCTTCTATCTGAGG+AGG - chr7_1:9034324-9034343 Msa1008570:intron 40.0%
AAGCCAAAGATGGCATTAAG+AGG - chr7_1:9034659-9034678 Msa1008570:CDS 40.0%
AGCCAAAGATGGCATTAAGA+GGG - chr7_1:9034660-9034679 Msa1008570:CDS 40.0%
TCCCATAAGAACTCATGACT+AGG - chr7_1:9034779-9034798 Msa1008570:CDS 40.0%
GCCTCACATAAACATGTTTC+TGG - chr7_1:9034807-9034826 Msa1008570:intron 40.0%
ATTCGCCAAATTCTTGTCGA+AGG - chr7_1:9034868-9034887 Msa1008570:intron 40.0%
!!! CGAAGGACTTTTTGGTTATG+TGG - chr7_1:9034885-9034904 Msa1008570:intron 40.0%
TGGTTTAGTCGAAAACGTTG+AGG + chr7_1:9034944-9034963 None:intergenic 40.0%
TCCCTTCATCAATGCATCAA+AGG + chr7_1:9034987-9035006 None:intergenic 40.0%
AAGGGACAATATCTACCCTA+TGG - chr7_1:9035001-9035020 Msa1008570:CDS 40.0%
ATTGTCTACTTGGAAGGGAA+TGG + chr7_1:9035029-9035048 None:intergenic 40.0%
TTGTGACGTCAAAAAGGCCA+AGG - chr7_1:9033999-9034018 Msa1008570:CDS 45.0%
ATCTGAGGAGGAACAATCTC+TGG - chr7_1:9034336-9034355 Msa1008570:intron 45.0%
CTCCCTCTTAATGCCATCTT+TGG + chr7_1:9034665-9034684 None:intergenic 45.0%
TAAACCATCCTCTCTCCAGA+TGG - chr7_1:9034957-9034976 Msa1008570:CDS 45.0%
TGCATCAAAGGATTGCCATC+TGG + chr7_1:9034975-9034994 None:intergenic 45.0%
TGGAAGGGAATGGATCCATA+GGG + chr7_1:9035019-9035038 None:intergenic 45.0%
TTGGAAGGGAATGGATCCAT+AGG + chr7_1:9035020-9035039 None:intergenic 45.0%
TTGTGACGTCAAAAAGGCCA+AGG - chr7_1:9033999-9034018 Msa1008570:CDS 45.0%
ATCTGAGGAGGAACAATCTC+TGG - chr7_1:9034336-9034355 Msa1008570:intron 45.0%
CTCCCTCTTAATGCCATCTT+TGG + chr7_1:9034665-9034684 None:intergenic 45.0%
TAAACCATCCTCTCTCCAGA+TGG - chr7_1:9034957-9034976 Msa1008570:CDS 45.0%
TGCATCAAAGGATTGCCATC+TGG + chr7_1:9034975-9034994 None:intergenic 45.0%
TGGAAGGGAATGGATCCATA+GGG + chr7_1:9035019-9035038 None:intergenic 45.0%
TTGGAAGGGAATGGATCCAT+AGG + chr7_1:9035020-9035039 None:intergenic 45.0%
ATTGCCATCTGGAGAGAGGA+TGG + chr7_1:9034964-9034983 None:intergenic 50.0%
AAGGATTGCCATCTGGAGAG+AGG + chr7_1:9034968-9034987 None:intergenic 50.0%
ATTGCCATCTGGAGAGAGGA+TGG + chr7_1:9034964-9034983 None:intergenic 50.0%
AAGGATTGCCATCTGGAGAG+AGG + chr7_1:9034968-9034987 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 9033976 9035051 9033976 ID=Msa1008570;Name=Msa1008570
chr7_1 mRNA 9033976 9035051 9033976 ID=Msa1008570-mRNA-1;Parent=Msa1008570;Name=Msa1008570-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|0.66|1|1|3|0|142
chr7_1 exon 9034909 9035051 9034909 ID=Msa1008570-mRNA-1:exon:2604;Parent=Msa1008570-mRNA-1
chr7_1 exon 9034628 9034802 9034628 ID=Msa1008570-mRNA-1:exon:2603;Parent=Msa1008570-mRNA-1
chr7_1 exon 9033976 9034086 9033976 ID=Msa1008570-mRNA-1:exon:2602;Parent=Msa1008570-mRNA-1
chr7_1 CDS 9034909 9035051 9034909 ID=Msa1008570-mRNA-1:cds;Parent=Msa1008570-mRNA-1
chr7_1 CDS 9034628 9034802 9034628 ID=Msa1008570-mRNA-1:cds;Parent=Msa1008570-mRNA-1
chr7_1 CDS 9033976 9034086 9033976 ID=Msa1008570-mRNA-1:cds;Parent=Msa1008570-mRNA-1
Gene Sequence

>Msa1008570

ATGGAAGGACATTCTCAAGAACATTGTGACGTCAAAAAGGCCAAGGAGTTCGTCAATGAAGTTAAGCATAGATTTATTCAAGTTAACAGGAGGGATAAGTATGTTGACTTTTTGAATGTCTTGATGCATTACAAGAAACAAAGGATCGATGATGCAAATCTCAGTAAATTCTTGAAGGGATTATTTGAAGGTCATGGAGATCTTATATCAAGATTGAACTACTTCATGCCTGATGGATTTGAAATCAAGCTTCTATCTGAGGAGGAACAATCTCTGGAGCATTTTGTAGCCAAAAATGCGAAAGAATTTGTCAATGATCCTCAACGTTTTCGACTAAACCATCCTCTCTCCAGATGGCAATCCTTTGATGCATTGATGAAGGGACAATATCTACCCTATGGATCCATTCCCTTCCAAGTAGACAATTGA

Protein sequence

>Msa1008570

MEGHSQEHCDVKKAKEFVNEVKHRFIQVNRRDKYVDFLNVLMHYKKQRIDDANLSKFLKGLFEGHGDLISRLNYFMPDGFEIKLLSEEEQSLEHFVAKNAKEFVNDPQRFRLNHPLSRWQSFDALMKGQYLPYGSIPFQVDN*