Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009080 | AES61431.2 | 96.045 | 177 | 7 | 0 | 1 | 177 | 81 | 257 | 6.94e-125 | 363 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009080 | G7I8T1 | 96.045 | 177 | 7 | 0 | 1 | 177 | 81 | 257 | 3.32e-125 | 363 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1009080 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009080 | MtrunA17_Chr6g0475591 | 96.296 | 54 | 2 | 0 | 124 | 177 | 1 | 54 | 9.04e-33 | 112 |
| Msa1009080 | MtrunA17_Chr1g0172971 | 84.615 | 52 | 8 | 0 | 1 | 52 | 66 | 117 | 3.13e-23 | 95.9 |
| Msa1009080 | MtrunA17_Chr7g0258861 | 27.485 | 171 | 107 | 5 | 10 | 178 | 43 | 198 | 3.05e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATGTTGGCCTGTTGTT+AGG | 0.302249 | 7_1:+9963186 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 7_1:-9962920 | Msa1009080:CDS |
| TTACTTCTTGAAATGATCTC+AGG | 0.422944 | 7_1:+9962995 | None:intergenic |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 7_1:-9962907 | Msa1009080:CDS |
| ATGGGTATAAGATATATAGA+TGG | 0.430188 | 7_1:-9963065 | Msa1009080:CDS |
| CAAGAAGTAAATGAAGTTCA+AGG | 0.439524 | 7_1:-9962982 | Msa1009080:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 7_1:-9963117 | Msa1009080:CDS |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 7_1:-9962839 | Msa1009080:CDS |
| GCTCTAATAAATTCTAAAGC+TGG | 0.462891 | 7_1:-9962952 | Msa1009080:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 7_1:-9962919 | Msa1009080:CDS |
| TTTAAGTCTGAAAACCGAAA+AGG | 0.483010 | 7_1:+9962868 | None:intergenic |
| GTTTGCTTAATCCTAACAAC+AGG | 0.484661 | 7_1:-9963197 | Msa1009080:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 7_1:-9963234 | Msa1009080:CDS |
| CTCAGGTAACACAAATGCTC+TGG | 0.496027 | 7_1:+9963012 | None:intergenic |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | 7_1:-9963100 | Msa1009080:CDS |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 7_1:+9963123 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 7_1:-9963223 | Msa1009080:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.514358 | 7_1:+9963024 | None:intergenic |
| TGGGTATAAGATATATAGAT+GGG | 0.523615 | 7_1:-9963064 | Msa1009080:CDS |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 7_1:-9963268 | Msa1009080:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 7_1:-9963222 | Msa1009080:CDS |
| TTCACAAACTCAATAACCAA+TGG | 0.554620 | 7_1:+9963159 | None:intergenic |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 7_1:-9962826 | Msa1009080:CDS |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 7_1:-9962833 | Msa1009080:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 7_1:-9963058 | Msa1009080:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 7_1:-9962904 | Msa1009080:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 7_1:-9962913 | Msa1009080:CDS |
| ACCAATGGACTGTCTGATGT+TGG | 0.594809 | 7_1:+9963174 | None:intergenic |
| GCCAACATCAGACAGTCCAT+TGG | 0.606903 | 7_1:-9963175 | Msa1009080:CDS |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 7_1:-9962830 | Msa1009080:CDS |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 7_1:-9962899 | Msa1009080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | - | chr7_1:9962785-9962804 | Msa1009080:CDS | 20.0% |
| ! | ATGGGTATAAGATATATAGA+TGG | - | chr7_1:9962988-9963007 | Msa1009080:CDS | 25.0% |
| ! | TGGGTATAAGATATATAGAT+GGG | - | chr7_1:9962989-9963008 | Msa1009080:CDS | 25.0% |
| CGTTATGTTTGTAACAAACA+AGG | - | chr7_1:9962819-9962838 | Msa1009080:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | chr7_1:9962830-9962849 | Msa1009080:CDS | 30.0% | |
| AACAAACAAGGTTTCAAGAA+GGG | - | chr7_1:9962831-9962850 | Msa1009080:CDS | 30.0% | |
| TTCACAAACTCAATAACCAA+TGG | + | chr7_1:9962897-9962916 | None:intergenic | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | chr7_1:9962995-9963014 | Msa1009080:CDS | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | chr7_1:9963032-9963051 | None:intergenic | 30.0% |
| TTACTTCTTGAAATGATCTC+AGG | + | chr7_1:9963061-9963080 | None:intergenic | 30.0% | |
| CAAGAAGTAAATGAAGTTCA+AGG | - | chr7_1:9963071-9963090 | Msa1009080:CDS | 30.0% | |
| !! | GCTCTAATAAATTCTAAAGC+TGG | - | chr7_1:9963101-9963120 | Msa1009080:CDS | 30.0% |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr7_1:9963134-9963153 | Msa1009080:CDS | 30.0% | |
| TTTAAGTCTGAAAACCGAAA+AGG | + | chr7_1:9963188-9963207 | None:intergenic | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | - | chr7_1:9963214-9963233 | Msa1009080:CDS | 30.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | chr7_1:9962856-9962875 | Msa1009080:CDS | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | - | chr7_1:9962914-9962933 | Msa1009080:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | - | chr7_1:9962919-9962938 | Msa1009080:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | - | chr7_1:9962920-9962939 | Msa1009080:CDS | 35.0% | |
| !!! | AAGCTGGATGAAGTTTTGAA+TGG | - | chr7_1:9962969-9962988 | Msa1009080:CDS | 35.0% |
| !!! | AGCTGGATGAAGTTTTGAAT+GGG | - | chr7_1:9962970-9962989 | Msa1009080:CDS | 35.0% |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr7_1:9963133-9963152 | Msa1009080:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | chr7_1:9963140-9963159 | Msa1009080:CDS | 35.0% | |
| !! | AGCTGGAATATCTTTGAAGC+TGG | - | chr7_1:9962953-9962972 | Msa1009080:CDS | 40.0% |
| GACAATTCGTCAAAAGGAGA+TGG | - | chr7_1:9963220-9963239 | Msa1009080:CDS | 40.0% | |
| AATTCGTCAAAAGGAGATGG+TGG | - | chr7_1:9963223-9963242 | Msa1009080:CDS | 40.0% | |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | + | chr7_1:9962870-9962889 | None:intergenic | 45.0% |
| !! | ACCAATGGACTGTCTGATGT+TGG | + | chr7_1:9962882-9962901 | None:intergenic | 45.0% |
| GAAAAACCTGAGGAAAGGGT+TGG | - | chr7_1:9962924-9962943 | Msa1009080:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | + | chr7_1:9962933-9962952 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr7_1:9962936-9962955 | Msa1009080:CDS | 45.0% |
| CTCAGGTAACACAAATGCTC+TGG | + | chr7_1:9963044-9963063 | None:intergenic | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | - | chr7_1:9963146-9963165 | Msa1009080:CDS | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+CGG | - | chr7_1:9963171-9963190 | Msa1009080:CDS | 45.0% |
| GCCAACATCAGACAGTCCAT+TGG | - | chr7_1:9962878-9962897 | Msa1009080:CDS | 50.0% | |
| GTTATGGGTCAAGGAGTTGG+AGG | - | chr7_1:9963149-9963168 | Msa1009080:CDS | 50.0% | |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | - | chr7_1:9963227-9963246 | Msa1009080:CDS | 50.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | chr7_1:9963154-9963173 | Msa1009080:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 9962762 | 9963313 | 9962762 | ID=Msa1009080;Name=Msa1009080 |
| chr7_1 | mRNA | 9962762 | 9963313 | 9962762 | ID=Msa1009080-mRNA-1;Parent=Msa1009080;Name=Msa1009080-mRNA-1;_AED=0.15;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|183 |
| chr7_1 | exon | 9962762 | 9963313 | 9962762 | ID=Msa1009080-mRNA-1:exon:2795;Parent=Msa1009080-mRNA-1 |
| chr7_1 | CDS | 9962762 | 9963313 | 9962762 | ID=Msa1009080-mRNA-1:cds;Parent=Msa1009080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
