Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009360 | XP_013447650.1 | 56.818 | 88 | 32 | 1 | 25 | 106 | 192 | 279 | 1.53e-21 | 98.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009360 | A0A072TX13 | 56.818 | 88 | 32 | 1 | 25 | 106 | 192 | 279 | 7.30e-22 | 98.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009360 | MtrunA17_Chr7g0219831 | 56.818 | 88 | 32 | 1 | 25 | 106 | 192 | 279 | 1.41e-25 | 98.2 |
| Msa1009360 | MtrunA17_Chr7g0220711 | 51.111 | 90 | 39 | 3 | 23 | 111 | 186 | 271 | 9.51e-18 | 76.6 |
| Msa1009360 | MtrunA17_Chr7g0219841 | 42.857 | 77 | 41 | 1 | 33 | 106 | 175 | 251 | 3.03e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGTGATGTTTACTCTTT+TGG | 0.218762 | 7_1:-10715119 | Msa1009360:CDS |
| TATTTGTGAAAGTATTTGTT+CGG | 0.416283 | 7_1:+10715141 | None:intergenic |
| GGATAAGATGAACATGTTCA+TGG | 0.455018 | 7_1:-10715048 | Msa1009360:CDS |
| AAACGACAAGTATACGATCT+TGG | 0.483113 | 7_1:-10715069 | Msa1009360:CDS |
| CATCCAACATCACCTTCATC+TGG | 0.510993 | 7_1:+10716016 | None:intergenic |
| CGACCAGATGAAGGTGATGT+TGG | 0.541871 | 7_1:-10716019 | Msa1009360:CDS |
| CAAAATTGAGTTCTTAGAAG+AGG | 0.555297 | 7_1:-10715024 | Msa1009360:CDS |
| TTGACAGGTACAACTGGCCA+TGG | 0.557462 | 7_1:-10715185 | Msa1009360:intron |
| TACAACTGGCCATGGTTACA+CGG | 0.562335 | 7_1:-10715177 | Msa1009360:CDS |
| GTATTTGTTCGGACACACTC+TGG | 0.574638 | 7_1:+10715152 | None:intergenic |
| TGTGAGCGTCGACCAGATGA+AGG | 0.576987 | 7_1:-10716028 | Msa1009360:CDS |
| ACTCTGGCGCCGTGTAACCA+TGG | 0.579905 | 7_1:+10715168 | None:intergenic |
| AAGGTGATGTTGGATGGTGT+CGG | 0.604264 | 7_1:-10716009 | Msa1009360:CDS |
| TGATGTTGGATGGTGTCGGA+TGG | 0.611461 | 7_1:-10716005 | Msa1009360:CDS |
| ACACAAAACATACAAAGTCA+AGG | 0.616287 | 7_1:-10715967 | Msa1009360:CDS |
| CAGATGAAGGTGATGTTGGA+TGG | 0.623760 | 7_1:-10716015 | Msa1009360:CDS |
| TACAAAGTCAAGGACGACAA+TGG | 0.654545 | 7_1:-10715957 | Msa1009360:CDS |
| ATGGCAAGACTGATATATGT+TGG | 0.661397 | 7_1:-10715938 | Msa1009360:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAGAATTTTTTTAGATAT+AGG | - | chr7_1:10715235-10715254 | Msa1009360:intron | 10.0% |
| !!! | TAAAGAATTTTTTTAGATAT+AGG | - | chr7_1:10715235-10715254 | Msa1009360:intron | 10.0% |
| !! | TTCTTCATGAAATTAAAAAT+TGG | - | chr7_1:10715183-10715202 | Msa1009360:intron | 15.0% |
| !!! | ATTTTTTTAGATATAGGATA+TGG | - | chr7_1:10715241-10715260 | Msa1009360:intron | 15.0% |
| !! | ATAGAGTATACATTTATATT+AGG | - | chr7_1:10715589-10715608 | Msa1009360:intron | 15.0% |
| !!! | AAAATTCTGAAGAAATAAAA+TGG | - | chr7_1:10715764-10715783 | Msa1009360:intron | 15.0% |
| !! | TTCTTCATGAAATTAAAAAT+TGG | - | chr7_1:10715183-10715202 | Msa1009360:intron | 15.0% |
| !!! | ATTTTTTTAGATATAGGATA+TGG | - | chr7_1:10715241-10715260 | Msa1009360:intron | 15.0% |
| !! | ATAGAGTATACATTTATATT+AGG | - | chr7_1:10715589-10715608 | Msa1009360:intron | 15.0% |
| !!! | AAAATTCTGAAGAAATAAAA+TGG | - | chr7_1:10715764-10715783 | Msa1009360:intron | 15.0% |
| !! | GAATAAAAAAAAACAGTTTG+TGG | + | chr7_1:10715146-10715165 | None:intergenic | 20.0% |
| !!! | TTAAAAATTGGCAAGATTTT+AGG | - | chr7_1:10715195-10715214 | Msa1009360:intron | 20.0% |
| !! | GTGAATCAAAAATAATGTTA+CGG | + | chr7_1:10715382-10715401 | None:intergenic | 20.0% |
| !!! | AAATATCCTTACTTTTAATG+TGG | - | chr7_1:10715615-10715634 | Msa1009360:intron | 20.0% |
| !! | TTCAATGAAAATGATAAAGT+TGG | - | chr7_1:10715651-10715670 | Msa1009360:intron | 20.0% |
| !!! | TATTTGTGAAAGTATTTGTT+CGG | + | chr7_1:10715888-10715907 | None:intergenic | 20.0% |
| !! | GAATAAAAAAAAACAGTTTG+TGG | + | chr7_1:10715146-10715165 | None:intergenic | 20.0% |
| !!! | TTAAAAATTGGCAAGATTTT+AGG | - | chr7_1:10715195-10715214 | Msa1009360:intron | 20.0% |
| !! | GTGAATCAAAAATAATGTTA+CGG | + | chr7_1:10715382-10715401 | None:intergenic | 20.0% |
| !!! | AAATATCCTTACTTTTAATG+TGG | - | chr7_1:10715615-10715634 | Msa1009360:intron | 20.0% |
| !! | TTCAATGAAAATGATAAAGT+TGG | - | chr7_1:10715651-10715670 | Msa1009360:intron | 20.0% |
| !!! | TATTTGTGAAAGTATTTGTT+CGG | + | chr7_1:10715888-10715907 | None:intergenic | 20.0% |
| ! | CTCAAATTCTACATTAGAAA+AGG | + | chr7_1:10715521-10715540 | None:intergenic | 25.0% |
| !!! | AATACACTAGTCTTTTAAGA+AGG | + | chr7_1:10715708-10715727 | None:intergenic | 25.0% |
| !! | AAATGTGATGTTTACTCTTT+TGG | - | chr7_1:10715907-10715926 | Msa1009360:intron | 25.0% |
| ! | CTCAAATTCTACATTAGAAA+AGG | + | chr7_1:10715521-10715540 | None:intergenic | 25.0% |
| !!! | AATACACTAGTCTTTTAAGA+AGG | + | chr7_1:10715708-10715727 | None:intergenic | 25.0% |
| !! | AAATGTGATGTTTACTCTTT+TGG | - | chr7_1:10715907-10715926 | Msa1009360:intron | 25.0% |
| ACACAAAACATACAAAGTCA+AGG | - | chr7_1:10715059-10715078 | Msa1009360:CDS | 30.0% | |
| TTAGAACATGTGCAGATTAA+AGG | - | chr7_1:10715298-10715317 | Msa1009360:intron | 30.0% | |
| !! | AGAGAGTCATTTTAAATCAG+AGG | - | chr7_1:10715325-10715344 | Msa1009360:intron | 30.0% |
| ! | AATAATGTTACGGAGTTGAT+TGG | + | chr7_1:10715372-10715391 | None:intergenic | 30.0% |
| TAAGTGCCACATTAAAAGTA+AGG | + | chr7_1:10715624-10715643 | None:intergenic | 30.0% | |
| ! | TGATGTTTACTCTTTTGGTA+CGG | - | chr7_1:10715912-10715931 | Msa1009360:intron | 30.0% |
| CAAAATTGAGTTCTTAGAAG+AGG | - | chr7_1:10716002-10716021 | Msa1009360:CDS | 30.0% | |
| ACACAAAACATACAAAGTCA+AGG | - | chr7_1:10715059-10715078 | Msa1009360:CDS | 30.0% | |
| TTAGAACATGTGCAGATTAA+AGG | - | chr7_1:10715298-10715317 | Msa1009360:intron | 30.0% | |
| !! | AGAGAGTCATTTTAAATCAG+AGG | - | chr7_1:10715325-10715344 | Msa1009360:intron | 30.0% |
| ! | AATAATGTTACGGAGTTGAT+TGG | + | chr7_1:10715372-10715391 | None:intergenic | 30.0% |
| TAAGTGCCACATTAAAAGTA+AGG | + | chr7_1:10715624-10715643 | None:intergenic | 30.0% | |
| ! | TGATGTTTACTCTTTTGGTA+CGG | - | chr7_1:10715912-10715931 | Msa1009360:intron | 30.0% |
| CAAAATTGAGTTCTTAGAAG+AGG | - | chr7_1:10716002-10716021 | Msa1009360:CDS | 30.0% | |
| ATGGCAAGACTGATATATGT+TGG | - | chr7_1:10715088-10715107 | Msa1009360:CDS | 35.0% | |
| CAAGACTGATATATGTTGGT+TGG | - | chr7_1:10715092-10715111 | Msa1009360:CDS | 35.0% | |
| !!! | AAGTTATTTTTCACCCGATG+AGG | - | chr7_1:10715115-10715134 | Msa1009360:CDS | 35.0% |
| AGTTTGTGGATATTCCTCAT+CGG | + | chr7_1:10715132-10715151 | None:intergenic | 35.0% | |
| !! | TGATTGGTGTCAAGTATAGT+CGG | + | chr7_1:10715356-10715375 | None:intergenic | 35.0% |
| TGTTGTTGTGTATCCTTACT+AGG | - | chr7_1:10715457-10715476 | Msa1009360:intron | 35.0% | |
| ! | TGATGCTTTTCATCCTAGTA+AGG | + | chr7_1:10715473-10715492 | None:intergenic | 35.0% |
| !! | TTTTAAGAAGGAGAAACCCA+GGG | + | chr7_1:10715696-10715715 | None:intergenic | 35.0% |
| TTCATAATTAGTGCTCGAGA+AGG | + | chr7_1:10715741-10715760 | None:intergenic | 35.0% | |
| ! | GATAGAATGCTTTTGTTGAC+AGG | - | chr7_1:10715826-10715845 | Msa1009360:intron | 35.0% |
| AAACGACAAGTATACGATCT+TGG | - | chr7_1:10715957-10715976 | Msa1009360:CDS | 35.0% | |
| GGATAAGATGAACATGTTCA+TGG | - | chr7_1:10715978-10715997 | Msa1009360:CDS | 35.0% | |
| ATGGCAAGACTGATATATGT+TGG | - | chr7_1:10715088-10715107 | Msa1009360:CDS | 35.0% | |
| CAAGACTGATATATGTTGGT+TGG | - | chr7_1:10715092-10715111 | Msa1009360:CDS | 35.0% | |
| !!! | AAGTTATTTTTCACCCGATG+AGG | - | chr7_1:10715115-10715134 | Msa1009360:CDS | 35.0% |
| AGTTTGTGGATATTCCTCAT+CGG | + | chr7_1:10715132-10715151 | None:intergenic | 35.0% | |
| !! | TGATTGGTGTCAAGTATAGT+CGG | + | chr7_1:10715356-10715375 | None:intergenic | 35.0% |
| TGTTGTTGTGTATCCTTACT+AGG | - | chr7_1:10715457-10715476 | Msa1009360:intron | 35.0% | |
| ! | TGATGCTTTTCATCCTAGTA+AGG | + | chr7_1:10715473-10715492 | None:intergenic | 35.0% |
| !! | TTTTAAGAAGGAGAAACCCA+GGG | + | chr7_1:10715696-10715715 | None:intergenic | 35.0% |
| TTCATAATTAGTGCTCGAGA+AGG | + | chr7_1:10715741-10715760 | None:intergenic | 35.0% | |
| ! | GATAGAATGCTTTTGTTGAC+AGG | - | chr7_1:10715826-10715845 | Msa1009360:intron | 35.0% |
| AAACGACAAGTATACGATCT+TGG | - | chr7_1:10715957-10715976 | Msa1009360:CDS | 35.0% | |
| GGATAAGATGAACATGTTCA+TGG | - | chr7_1:10715978-10715997 | Msa1009360:CDS | 35.0% | |
| TACAAAGTCAAGGACGACAA+TGG | - | chr7_1:10715069-10715088 | Msa1009360:CDS | 40.0% | |
| GTTTGTGGATATTCCTCATC+GGG | + | chr7_1:10715131-10715150 | None:intergenic | 40.0% | |
| TACCTTACGCCTAGTACTAT+AGG | - | chr7_1:10715553-10715572 | Msa1009360:intron | 40.0% | |
| ACCTTACGCCTAGTACTATA+GGG | - | chr7_1:10715554-10715573 | Msa1009360:intron | 40.0% | |
| CAAGTCAACCCTATAGTACT+AGG | + | chr7_1:10715565-10715584 | None:intergenic | 40.0% | |
| CTAGTACTATAGGGTTGACT+TGG | - | chr7_1:10715563-10715582 | Msa1009360:intron | 40.0% | |
| ATGATAATCCCTAAATGCCC+TGG | - | chr7_1:10715676-10715695 | Msa1009360:intron | 40.0% | |
| TGATAATCCCTAAATGCCCT+GGG | - | chr7_1:10715677-10715696 | Msa1009360:intron | 40.0% | |
| !! | CTTTTAAGAAGGAGAAACCC+AGG | + | chr7_1:10715697-10715716 | None:intergenic | 40.0% |
| ! | CTTTTGTTGACAGGTACAAC+TGG | - | chr7_1:10715835-10715854 | Msa1009360:intron | 40.0% |
| TACAAAGTCAAGGACGACAA+TGG | - | chr7_1:10715069-10715088 | Msa1009360:CDS | 40.0% | |
| GTTTGTGGATATTCCTCATC+GGG | + | chr7_1:10715131-10715150 | None:intergenic | 40.0% | |
| TACCTTACGCCTAGTACTAT+AGG | - | chr7_1:10715553-10715572 | Msa1009360:intron | 40.0% | |
| ACCTTACGCCTAGTACTATA+GGG | - | chr7_1:10715554-10715573 | Msa1009360:intron | 40.0% | |
| CAAGTCAACCCTATAGTACT+AGG | + | chr7_1:10715565-10715584 | None:intergenic | 40.0% | |
| CTAGTACTATAGGGTTGACT+TGG | - | chr7_1:10715563-10715582 | Msa1009360:intron | 40.0% | |
| ATGATAATCCCTAAATGCCC+TGG | - | chr7_1:10715676-10715695 | Msa1009360:intron | 40.0% | |
| TGATAATCCCTAAATGCCCT+GGG | - | chr7_1:10715677-10715696 | Msa1009360:intron | 40.0% | |
| !! | CTTTTAAGAAGGAGAAACCC+AGG | + | chr7_1:10715697-10715716 | None:intergenic | 40.0% |
| ! | CTTTTGTTGACAGGTACAAC+TGG | - | chr7_1:10715835-10715854 | Msa1009360:intron | 40.0% |
| CATCCAACATCACCTTCATC+TGG | + | chr7_1:10715013-10715032 | None:intergenic | 45.0% | |
| CAGATGAAGGTGATGTTGGA+TGG | - | chr7_1:10715011-10715030 | Msa1009360:CDS | 45.0% | |
| AAGGTGATGTTGGATGGTGT+CGG | - | chr7_1:10715017-10715036 | Msa1009360:CDS | 45.0% | |
| ACCCTATAGTACTAGGCGTA+AGG | + | chr7_1:10715558-10715577 | None:intergenic | 45.0% | |
| TACAACTGGCCATGGTTACA+CGG | - | chr7_1:10715849-10715868 | Msa1009360:intron | 45.0% | |
| GTATTTGTTCGGACACACTC+TGG | + | chr7_1:10715877-10715896 | None:intergenic | 45.0% | |
| CATCCAACATCACCTTCATC+TGG | + | chr7_1:10715013-10715032 | None:intergenic | 45.0% | |
| CAGATGAAGGTGATGTTGGA+TGG | - | chr7_1:10715011-10715030 | Msa1009360:CDS | 45.0% | |
| AAGGTGATGTTGGATGGTGT+CGG | - | chr7_1:10715017-10715036 | Msa1009360:CDS | 45.0% | |
| ACCCTATAGTACTAGGCGTA+AGG | + | chr7_1:10715558-10715577 | None:intergenic | 45.0% | |
| TACAACTGGCCATGGTTACA+CGG | - | chr7_1:10715849-10715868 | Msa1009360:intron | 45.0% | |
| GTATTTGTTCGGACACACTC+TGG | + | chr7_1:10715877-10715896 | None:intergenic | 45.0% | |
| CGACCAGATGAAGGTGATGT+TGG | - | chr7_1:10715007-10715026 | Msa1009360:CDS | 50.0% | |
| ! | TGATGTTGGATGGTGTCGGA+TGG | - | chr7_1:10715021-10715040 | Msa1009360:CDS | 50.0% |
| ! | GGAGAAACCCAGGGCATTTA+GGG | + | chr7_1:10715687-10715706 | None:intergenic | 50.0% |
| ! | AGGAGAAACCCAGGGCATTT+AGG | + | chr7_1:10715688-10715707 | None:intergenic | 50.0% |
| TTGACAGGTACAACTGGCCA+TGG | - | chr7_1:10715841-10715860 | Msa1009360:intron | 50.0% | |
| CGACCAGATGAAGGTGATGT+TGG | - | chr7_1:10715007-10715026 | Msa1009360:CDS | 50.0% | |
| ! | TGATGTTGGATGGTGTCGGA+TGG | - | chr7_1:10715021-10715040 | Msa1009360:CDS | 50.0% |
| ! | GGAGAAACCCAGGGCATTTA+GGG | + | chr7_1:10715687-10715706 | None:intergenic | 50.0% |
| ! | AGGAGAAACCCAGGGCATTT+AGG | + | chr7_1:10715688-10715707 | None:intergenic | 50.0% |
| TTGACAGGTACAACTGGCCA+TGG | - | chr7_1:10715841-10715860 | Msa1009360:intron | 50.0% | |
| !! | TGTGAGCGTCGACCAGATGA+AGG | - | chr7_1:10714998-10715017 | Msa1009360:CDS | 55.0% |
| !! | TGTGAGCGTCGACCAGATGA+AGG | - | chr7_1:10714998-10715017 | Msa1009360:CDS | 55.0% |
| ACTCTGGCGCCGTGTAACCA+TGG | + | chr7_1:10715861-10715880 | None:intergenic | 60.0% | |
| ACTCTGGCGCCGTGTAACCA+TGG | + | chr7_1:10715861-10715880 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 10714983 | 10716065 | 10714983 | ID=Msa1009360;Name=Msa1009360 |
| chr7_1 | mRNA | 10714983 | 10716065 | 10714983 | ID=Msa1009360-mRNA-1;Parent=Msa1009360;Name=Msa1009360-mRNA-1;_AED=0.61;_eAED=0.66;_QI=0|0|0|0.5|1|1|2|0|114 |
| chr7_1 | exon | 10715939 | 10716065 | 10715939 | ID=Msa1009360-mRNA-1:exon:2893;Parent=Msa1009360-mRNA-1 |
| chr7_1 | exon | 10714983 | 10715200 | 10714983 | ID=Msa1009360-mRNA-1:exon:2892;Parent=Msa1009360-mRNA-1 |
| chr7_1 | CDS | 10715939 | 10716065 | 10715939 | ID=Msa1009360-mRNA-1:cds;Parent=Msa1009360-mRNA-1 |
| chr7_1 | CDS | 10714983 | 10715200 | 10714983 | ID=Msa1009360-mRNA-1:cds;Parent=Msa1009360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
