Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013450 | AES99309.1 | 48.521 | 169 | 35 | 2 | 1 | 117 | 138 | 306 | 4.14e-41 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013450 | sp|A0A2R6S148|MYB1_ACTCC | 55.556 | 54 | 23 | 1 | 1 | 54 | 85 | 137 | 6.69e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013450 | G7K1W1 | 48.521 | 169 | 35 | 2 | 1 | 117 | 138 | 306 | 1.98e-41 | 148 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1013450 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013450 | MtrunA17_Chr7g0229101 | 53.659 | 164 | 26 | 6 | 1 | 117 | 49 | 209 | 3.34e-44 | 142 |
| Msa1013450 | MtrunA17_Chr8g0360111 | 41.250 | 160 | 51 | 2 | 1 | 117 | 102 | 261 | 5.95e-30 | 107 |
| Msa1013450 | MtrunA17_Chr5g0434861 | 53.846 | 91 | 39 | 2 | 29 | 117 | 31 | 120 | 7.66e-24 | 88.6 |
| Msa1013450 | MtrunA17_Chr7g0228991 | 37.209 | 172 | 49 | 4 | 1 | 116 | 106 | 274 | 2.77e-21 | 85.5 |
| Msa1013450 | MtrunA17_Chr5g0434931 | 39.535 | 172 | 45 | 5 | 1 | 114 | 112 | 282 | 3.00e-21 | 85.5 |
| Msa1013450 | MtrunA17_Chr7g0220341 | 58.571 | 70 | 28 | 1 | 48 | 117 | 207 | 275 | 1.19e-19 | 81.3 |
| Msa1013450 | MtrunA17_Chr7g0220341 | 77.778 | 45 | 8 | 1 | 1 | 45 | 104 | 146 | 2.10e-15 | 69.7 |
| Msa1013450 | MtrunA17_Chr7g0229131 | 62.500 | 56 | 21 | 0 | 1 | 56 | 49 | 104 | 4.21e-17 | 72.4 |
| Msa1013450 | MtrunA17_Chr5g0434891 | 37.008 | 127 | 45 | 3 | 1 | 92 | 13 | 139 | 8.06e-16 | 68.6 |
| Msa1013450 | MtrunA17_Chr5g0434871 | 90.000 | 30 | 3 | 0 | 1 | 30 | 17 | 46 | 3.48e-14 | 62.4 |
| Msa1013450 | MtrunA17_Chr5g0434911 | 87.097 | 31 | 4 | 0 | 1 | 31 | 98 | 128 | 1.07e-12 | 62.4 |
| Msa1013450 | MtrunA17_Chr7g0240821 | 49.153 | 59 | 25 | 1 | 1 | 59 | 78 | 131 | 5.33e-12 | 60.1 |
| Msa1013450 | MtrunA17_Chr4g0055981 | 60.870 | 46 | 17 | 1 | 1 | 46 | 132 | 176 | 1.27e-11 | 58.9 |
| Msa1013450 | MtrunA17_Chr5g0434831 | 80.645 | 31 | 6 | 0 | 1 | 31 | 84 | 114 | 1.85e-11 | 58.2 |
| Msa1013450 | MtrunA17_Chr5g0434841 | 86.207 | 29 | 4 | 0 | 1 | 29 | 97 | 125 | 2.86e-11 | 58.9 |
| Msa1013450 | MtrunA17_Chr4g0037141 | 75.862 | 29 | 7 | 0 | 1 | 29 | 89 | 117 | 4.03e-11 | 58.2 |
| Msa1013450 | MtrunA17_Chr3g0121241 | 70.968 | 31 | 9 | 0 | 1 | 31 | 119 | 149 | 6.72e-11 | 57.8 |
| Msa1013450 | MtrunA17_Chr4g0044421 | 70.968 | 31 | 9 | 0 | 1 | 31 | 89 | 119 | 6.81e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1013450 | AT1G66380.3 | 96.296 | 27 | 1 | 0 | 1 | 27 | 52 | 78 | 1.54e-12 | 59.7 |
| Msa1013450 | AT4G09460.1 | 48.148 | 54 | 28 | 0 | 1 | 54 | 89 | 142 | 1.75e-12 | 62.0 |
| Msa1013450 | AT1G66380.1 | 96.296 | 27 | 1 | 0 | 1 | 27 | 85 | 111 | 3.55e-12 | 59.3 |
| Msa1013450 | AT1G66390.1 | 96.296 | 27 | 1 | 0 | 1 | 27 | 85 | 111 | 9.60e-12 | 60.1 |
| Msa1013450 | AT1G56650.1 | 96.296 | 27 | 1 | 0 | 1 | 27 | 85 | 111 | 1.02e-11 | 60.1 |
| Msa1013450 | AT1G66370.1 | 96.296 | 27 | 1 | 0 | 1 | 27 | 85 | 111 | 1.08e-11 | 59.7 |
| Msa1013450 | AT3G13540.1 | 74.194 | 31 | 8 | 0 | 1 | 31 | 100 | 130 | 1.92e-11 | 59.3 |
| Msa1013450 | AT4G38620.1 | 75.862 | 29 | 7 | 0 | 1 | 29 | 89 | 117 | 2.30e-11 | 59.3 |
| Msa1013450 | AT1G22640.2 | 73.333 | 30 | 8 | 0 | 1 | 30 | 17 | 46 | 2.98e-11 | 57.8 |
| Msa1013450 | AT1G22640.3 | 73.333 | 30 | 8 | 0 | 1 | 30 | 32 | 61 | 3.76e-11 | 57.8 |
| Msa1013450 | AT1G22640.1 | 73.333 | 30 | 8 | 0 | 1 | 30 | 89 | 118 | 8.44e-11 | 57.4 |
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCACATTGCACATGGTATT+TGG | 0.146488 | 7_1:+20102671 | None:intergenic |
| CAATGTGGTCAGACAGTTTA+TGG | 0.252718 | 7_1:-20102657 | Msa1013450:CDS |
| TTGATTGCTGGAAGGCTTCC+AGG | 0.298424 | 7_1:-20102929 | Msa1013450:CDS |
| AGAAAAGTTGAAATCCAAAA+AGG | 0.363874 | 7_1:+20102543 | None:intergenic |
| AATGTGGTCAGACAGTTTAT+GGG | 0.473171 | 7_1:-20102656 | Msa1013450:CDS |
| TACAGATGGTCATTGATTGC+TGG | 0.478657 | 7_1:-20102941 | None:intergenic |
| GAACACACATTTGCGGAAGA+AGG | 0.508101 | 7_1:-20102876 | Msa1013450:CDS |
| GTCAGACAGTTTATGGGACA+TGG | 0.511094 | 7_1:-20102650 | Msa1013450:CDS |
| ACATCATTAGCTGTTCTACC+TGG | 0.537311 | 7_1:+20102911 | None:intergenic |
| ATTTGGACATTACCAAGACT+TGG | 0.537965 | 7_1:+20102688 | None:intergenic |
| ACTATTGGAACACACATTTG+CGG | 0.543348 | 7_1:-20102883 | Msa1013450:CDS |
| TCTGCTGTACAACCAAGTCT+TGG | 0.544122 | 7_1:-20102700 | Msa1013450:CDS |
| CTGTCTGACCACATTGCACA+TGG | 0.580512 | 7_1:+20102664 | None:intergenic |
| TTCTTGTAATGAAGAGCATG+AGG | 0.612500 | 7_1:+20102600 | None:intergenic |
| GATGGTCATTGATTGCTGGA+AGG | 0.633657 | 7_1:-20102937 | Msa1013450:CDS |
| TCAGACAGTTTATGGGACAT+GGG | 0.638905 | 7_1:-20102649 | Msa1013450:CDS |
| CAGACAGTTTATGGGACATG+GGG | 0.654061 | 7_1:-20102648 | Msa1013450:CDS |
| TCCAAATACCATGTGCAATG+TGG | 0.694169 | 7_1:-20102672 | Msa1013450:CDS |
| AGACAGTTTATGGGACATGG+GGG | 0.716102 | 7_1:-20102647 | Msa1013450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAGAAAAAGAGAAATCTA+AGG | - | chr7_1:20102623-20102642 | Msa1013450:CDS | 20.0% |
| ! | CTAATGATGTGAAAAACTAT+TGG | - | chr7_1:20102556-20102575 | Msa1013450:CDS | 25.0% |
| ! | GAACGAGATGATAAATTTAT+AGG | + | chr7_1:20102683-20102702 | None:intergenic | 25.0% |
| ! | AGAAAAGTTGAAATCCAAAA+AGG | + | chr7_1:20102914-20102933 | None:intergenic | 25.0% |
| !! | TTTTCTTGATGACTCCTTTT+TGG | - | chr7_1:20102897-20102916 | Msa1013450:CDS | 30.0% |
| ACTATTGGAACACACATTTG+CGG | - | chr7_1:20102571-20102590 | Msa1013450:CDS | 35.0% | |
| GCTCATGAGGTTATTAAACA+CGG | - | chr7_1:20102657-20102676 | Msa1013450:CDS | 35.0% | |
| ATTTGGACATTACCAAGACT+TGG | + | chr7_1:20102769-20102788 | None:intergenic | 35.0% | |
| AATGTGGTCAGACAGTTTAT+GGG | - | chr7_1:20102798-20102817 | Msa1013450:intron | 35.0% | |
| ! | TTCTTGTAATGAAGAGCATG+AGG | + | chr7_1:20102857-20102876 | None:intergenic | 35.0% |
| ACATCATTAGCTGTTCTACC+TGG | + | chr7_1:20102546-20102565 | None:intergenic | 40.0% | |
| GGAAACAATGTAAGCTCATG+AGG | - | chr7_1:20102644-20102663 | Msa1013450:CDS | 40.0% | |
| TCCAAATACCATGTGCAATG+TGG | - | chr7_1:20102782-20102801 | Msa1013450:intron | 40.0% | |
| ACCACATTGCACATGGTATT+TGG | + | chr7_1:20102786-20102805 | None:intergenic | 40.0% | |
| CAATGTGGTCAGACAGTTTA+TGG | - | chr7_1:20102797-20102816 | Msa1013450:intron | 40.0% | |
| TCAGACAGTTTATGGGACAT+GGG | - | chr7_1:20102805-20102824 | Msa1013450:intron | 40.0% | |
| GAACACACATTTGCGGAAGA+AGG | - | chr7_1:20102578-20102597 | Msa1013450:CDS | 45.0% | |
| TCTGCTGTACAACCAAGTCT+TGG | - | chr7_1:20102754-20102773 | Msa1013450:intron | 45.0% | |
| GTCAGACAGTTTATGGGACA+TGG | - | chr7_1:20102804-20102823 | Msa1013450:intron | 45.0% | |
| CAGACAGTTTATGGGACATG+GGG | - | chr7_1:20102806-20102825 | Msa1013450:intron | 45.0% | |
| AGACAGTTTATGGGACATGG+GGG | - | chr7_1:20102807-20102826 | Msa1013450:intron | 45.0% | |
| TTGATTGCTGGAAGGCTTCC+AGG | - | chr7_1:20102525-20102544 | Msa1013450:CDS | 50.0% | |
| CTGTCTGACCACATTGCACA+TGG | + | chr7_1:20102793-20102812 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 20102519 | 20102957 | 20102519 | ID=Msa1013450;Name=Msa1013450 |
| chr7_1 | mRNA | 20102519 | 20102957 | 20102519 | ID=Msa1013450-mRNA-1;Parent=Msa1013450;Name=Msa1013450-mRNA-1;_AED=0.11;_eAED=0.13;_QI=0|0|0|1|1|0|2|0|117 |
| chr7_1 | exon | 20102824 | 20102957 | 20102824 | ID=Msa1013450-mRNA-1:exon:4847;Parent=Msa1013450-mRNA-1 |
| chr7_1 | exon | 20102519 | 20102735 | 20102519 | ID=Msa1013450-mRNA-1:exon:4846;Parent=Msa1013450-mRNA-1 |
| chr7_1 | CDS | 20102824 | 20102957 | 20102824 | ID=Msa1013450-mRNA-1:cds;Parent=Msa1013450-mRNA-1 |
| chr7_1 | CDS | 20102519 | 20102735 | 20102519 | ID=Msa1013450-mRNA-1:cds;Parent=Msa1013450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
