AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1014160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1014160 MtrunA17_Chr2g0296781 66.667 135 45 0 48 182 3 137 1.51e-55 190
Msa1014160 MtrunA17_Chr1g0196541 47.368 171 88 1 48 216 3 173 3.60e-43 155
Msa1014160 MtrunA17_Chr6g0476431 61.628 86 33 0 48 133 3 88 3.97e-33 116
Msa1014160 MtrunA17_Chr6g0451441 32.022 178 111 5 10 181 52 225 5.43e-18 83.2
Msa1014160 MtrunA17_Chr2g0329031 34.167 120 71 3 48 160 20 138 5.44e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1014160 AT1G76320.2 36.792 106 64 2 48 151 3 107 7.46e-15 74.7
Msa1014160 AT1G76320.1 36.792 106 64 2 48 151 3 107 7.75e-15 74.3
Msa1014160 AT1G76320.4 36.792 106 64 2 48 151 3 107 7.75e-15 74.3
Msa1014160 AT1G76320.3 36.792 106 64 2 48 151 3 107 1.19e-14 73.9

Find 28 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTCATAAGCATTGAATT+TGG 0.196629 7_1:+22187327 None:intergenic
AATGCATGTATTTCTTTAGA+TGG 0.249459 7_1:-22187730 Msa1014160:CDS
CTAAAATAGCGTGCTTTGTC+AGG 0.272247 7_1:+22187658 None:intergenic
TATGCCCAATCGATGGGTTT+TGG 0.287180 7_1:-22187898 Msa1014160:CDS
TACCTGTAACAATTCAAAAT+AGG 0.377069 7_1:+22187175 None:intergenic
TGCATGACTTGAGAATAAAA+TGG 0.407764 7_1:+22187209 None:intergenic
GCTTATGAGTGAAGTAAATC+AGG 0.434328 7_1:-22187316 Msa1014160:intron
AAGCATTGAATTTGGATTGA+AGG 0.439628 7_1:+22187335 None:intergenic
AGCATTGAATTTGGATTGAA+GGG 0.452863 7_1:+22187336 None:intergenic
CATTCAATCTAGCCTTGCAT+TGG 0.455682 7_1:+22187749 None:intergenic
GTTCAAGTAGTTGATGTAGT+TGG 0.457215 7_1:-22187532 Msa1014160:CDS
AACTCCAAAACCCATCGATT+GGG 0.481007 7_1:+22187894 None:intergenic
ATTCAATCTAGCCTTGCATT+GGG 0.485892 7_1:+22187750 None:intergenic
TAACTCCAAAACCCATCGAT+TGG 0.508337 7_1:+22187893 None:intergenic
CAAGAGTTATGCCCAATCGA+TGG 0.518094 7_1:-22187905 Msa1014160:CDS
ATTTGTATCTTCTGATGAAG+AGG 0.520293 7_1:-22188046 Msa1014160:CDS
TATTCTCAAGTCATGCATCA+TGG 0.520944 7_1:-22187203 Msa1014160:CDS
AAGAGTTATGCCCAATCGAT+GGG 0.531992 7_1:-22187904 Msa1014160:CDS
TGGTTCCTCGTTTGAAAAGG+AGG 0.532707 7_1:-22187621 Msa1014160:CDS
TAAAATAGCGTGCTTTGTCA+GGG 0.562727 7_1:+22187659 None:intergenic
TTAGAACTCAATGATCAAGC+TGG 0.567016 7_1:-22187598 Msa1014160:CDS
TTTACTCTAGCATGTAGTTG+TGG 0.583557 7_1:-22187829 Msa1014160:CDS
CGTGTTATTGATGACGACAA+CGG 0.597290 7_1:+22187044 None:intergenic
GATGAAGAGGAAATCAGAGA+GGG 0.625109 7_1:-22188033 Msa1014160:CDS
CTAAATATAGTTCCAACCTG+AGG 0.648571 7_1:+22187955 None:intergenic
TTTGGGAGGAATGGACATGG+TGG 0.651520 7_1:-22187375 Msa1014160:CDS
TGATGAAGAGGAAATCAGAG+AGG 0.679814 7_1:-22188034 Msa1014160:CDS
ACGACAACGGATCATTCACA+TGG 0.697567 7_1:+22187057 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTGATTTATCATATAA+AGG + chr7_1:22187116-22187135 None:intergenic 10.0%
!!! TTTAATACATATATGTTTTT+AGG - chr7_1:22187914-22187933 Msa1014160:CDS 10.0%
!!! TTAATACATATATGTTTTTA+GGG - chr7_1:22187915-22187934 Msa1014160:CDS 10.0%
!!! AAAATTGATTTATCATATAA+AGG + chr7_1:22187116-22187135 None:intergenic 10.0%
!!! TTTAATACATATATGTTTTT+AGG - chr7_1:22187914-22187933 Msa1014160:CDS 10.0%
!!! TTAATACATATATGTTTTTA+GGG - chr7_1:22187915-22187934 Msa1014160:CDS 10.0%
!!! TTTTTAATTTATTGCATTAG+TGG - chr7_1:22187250-22187269 Msa1014160:intron 15.0%
!!! TAATACATATATGTTTTTAG+GGG - chr7_1:22187916-22187935 Msa1014160:CDS 15.0%
!!! TTTTTAATTTATTGCATTAG+TGG - chr7_1:22187250-22187269 Msa1014160:intron 15.0%
!!! TAATACATATATGTTTTTAG+GGG - chr7_1:22187916-22187935 Msa1014160:CDS 15.0%
!!! TTTTGATATTGAAACTGATT+TGG - chr7_1:22187139-22187158 Msa1014160:intron 20.0%
!! TAAGCATTAAATACAAATCT+AGG + chr7_1:22187170-22187189 None:intergenic 20.0%
!!! TTTTAGAAGTAGTAAGTTTT+TGG - chr7_1:22187685-22187704 Msa1014160:CDS 20.0%
!!! AATACATATATGTTTTTAGG+GGG - chr7_1:22187917-22187936 Msa1014160:CDS 20.0%
!! TTCATGTAACATTAATATTG+TGG - chr7_1:22188062-22188081 Msa1014160:intron 20.0%
!!! TTTTGATATTGAAACTGATT+TGG - chr7_1:22187139-22187158 Msa1014160:intron 20.0%
!! TAAGCATTAAATACAAATCT+AGG + chr7_1:22187170-22187189 None:intergenic 20.0%
!!! TTTTAGAAGTAGTAAGTTTT+TGG - chr7_1:22187685-22187704 Msa1014160:CDS 20.0%
!!! AATACATATATGTTTTTAGG+GGG - chr7_1:22187917-22187936 Msa1014160:CDS 20.0%
!! TTCATGTAACATTAATATTG+TGG - chr7_1:22188062-22188081 Msa1014160:intron 20.0%
! TAAGTTAACTACCTTAAACT+AGG - chr7_1:22187057-22187076 Msa1014160:CDS 25.0%
! AATGCATGTATTTCTTTAGA+TGG - chr7_1:22187596-22187615 Msa1014160:CDS 25.0%
!!! AAGCATTTTCCATGTTATTT+AGG - chr7_1:22188092-22188111 Msa1014160:intron 25.0%
! AAAATGGAACCTAAATAACA+TGG + chr7_1:22188104-22188123 None:intergenic 25.0%
!!! TTCCTATTTTGAATTGTTAC+AGG - chr7_1:22188149-22188168 Msa1014160:intron 25.0%
! TACCTGTAACAATTCAAAAT+AGG + chr7_1:22188154-22188173 None:intergenic 25.0%
!!! TGTTAGTTTGTTCTTTTTGT+AGG - chr7_1:22188239-22188258 Msa1014160:intron 25.0%
! TAAGTTAACTACCTTAAACT+AGG - chr7_1:22187057-22187076 Msa1014160:CDS 25.0%
! AATGCATGTATTTCTTTAGA+TGG - chr7_1:22187596-22187615 Msa1014160:CDS 25.0%
!!! AAGCATTTTCCATGTTATTT+AGG - chr7_1:22188092-22188111 Msa1014160:intron 25.0%
! AAAATGGAACCTAAATAACA+TGG + chr7_1:22188104-22188123 None:intergenic 25.0%
!!! TTCCTATTTTGAATTGTTAC+AGG - chr7_1:22188149-22188168 Msa1014160:intron 25.0%
! TACCTGTAACAATTCAAAAT+AGG + chr7_1:22188154-22188173 None:intergenic 25.0%
!!! TGTTAGTTTGTTCTTTTTGT+AGG - chr7_1:22188239-22188258 Msa1014160:intron 25.0%
ATTTGTATCTTCTGATGAAG+AGG - chr7_1:22187280-22187299 Msa1014160:intron 30.0%
AGTAACAAAAATGCAGATGA+TGG - chr7_1:22187464-22187483 Msa1014160:intron 30.0%
GTAACAAAAATGCAGATGAT+GGG - chr7_1:22187465-22187484 Msa1014160:intron 30.0%
! GGGTTTTTGTTAAAGGATTT+GGG + chr7_1:22187559-22187578 None:intergenic 30.0%
! TGGGTTTTTGTTAAAGGATT+TGG + chr7_1:22187560-22187579 None:intergenic 30.0%
! TTGCATTGGGTTTTTGTTAA+AGG + chr7_1:22187566-22187585 None:intergenic 30.0%
TTTAACAAAAACCCAATGCA+AGG - chr7_1:22187565-22187584 Msa1014160:CDS 30.0%
!! TAGGGGGAAAAAATTAGTTT+TGG - chr7_1:22187933-22187952 Msa1014160:CDS 30.0%
!!! AGGGGGAAAAAATTAGTTTT+GGG - chr7_1:22187934-22187953 Msa1014160:CDS 30.0%
!!! AAAATTAGTTTTGGGAGGAA+TGG - chr7_1:22187942-22187961 Msa1014160:CDS 30.0%
! AGCATTGAATTTGGATTGAA+GGG + chr7_1:22187993-22188012 None:intergenic 30.0%
!! AAGCATTGAATTTGGATTGA+AGG + chr7_1:22187994-22188013 None:intergenic 30.0%
!! TCACTCATAAGCATTGAATT+TGG + chr7_1:22188002-22188021 None:intergenic 30.0%
TGCATGACTTGAGAATAAAA+TGG + chr7_1:22188120-22188139 None:intergenic 30.0%
ATTTGTATCTTCTGATGAAG+AGG - chr7_1:22187280-22187299 Msa1014160:intron 30.0%
AGTAACAAAAATGCAGATGA+TGG - chr7_1:22187464-22187483 Msa1014160:intron 30.0%
GTAACAAAAATGCAGATGAT+GGG - chr7_1:22187465-22187484 Msa1014160:intron 30.0%
! GGGTTTTTGTTAAAGGATTT+GGG + chr7_1:22187559-22187578 None:intergenic 30.0%
! TGGGTTTTTGTTAAAGGATT+TGG + chr7_1:22187560-22187579 None:intergenic 30.0%
! TTGCATTGGGTTTTTGTTAA+AGG + chr7_1:22187566-22187585 None:intergenic 30.0%
TTTAACAAAAACCCAATGCA+AGG - chr7_1:22187565-22187584 Msa1014160:CDS 30.0%
!! TAGGGGGAAAAAATTAGTTT+TGG - chr7_1:22187933-22187952 Msa1014160:CDS 30.0%
!!! AGGGGGAAAAAATTAGTTTT+GGG - chr7_1:22187934-22187953 Msa1014160:CDS 30.0%
!!! AAAATTAGTTTTGGGAGGAA+TGG - chr7_1:22187942-22187961 Msa1014160:CDS 30.0%
! AGCATTGAATTTGGATTGAA+GGG + chr7_1:22187993-22188012 None:intergenic 30.0%
!! AAGCATTGAATTTGGATTGA+AGG + chr7_1:22187994-22188013 None:intergenic 30.0%
!! TCACTCATAAGCATTGAATT+TGG + chr7_1:22188002-22188021 None:intergenic 30.0%
TGCATGACTTGAGAATAAAA+TGG + chr7_1:22188120-22188139 None:intergenic 30.0%
ATGGGTGATAACCTAGTTTA+AGG + chr7_1:22187071-22187090 None:intergenic 35.0%
!! GTAGTTTGATCAGTTGATGA+GGG + chr7_1:22187094-22187113 None:intergenic 35.0%
TCATGAAGATGAAAAACCTC+AGG - chr7_1:22187355-22187374 Msa1014160:CDS 35.0%
CTAAATATAGTTCCAACCTG+AGG + chr7_1:22187374-22187393 None:intergenic 35.0%
TTTACTCTAGCATGTAGTTG+TGG - chr7_1:22187497-22187516 Msa1014160:intron 35.0%
!! ATTCAATCTAGCCTTGCATT+GGG + chr7_1:22187579-22187598 None:intergenic 35.0%
TAAAATAGCGTGCTTTGTCA+GGG + chr7_1:22187670-22187689 None:intergenic 35.0%
!!! TTTTGGTTCCTCGTTTGAAA+AGG - chr7_1:22187702-22187721 Msa1014160:CDS 35.0%
TTAGAACTCAATGATCAAGC+TGG - chr7_1:22187728-22187747 Msa1014160:CDS 35.0%
! GTTCAAGTAGTTGATGTAGT+TGG - chr7_1:22187794-22187813 Msa1014160:CDS 35.0%
!!! GGGAAAAAATTAGTTTTGGG+AGG - chr7_1:22187937-22187956 Msa1014160:CDS 35.0%
GCTTATGAGTGAAGTAAATC+AGG - chr7_1:22188010-22188029 Msa1014160:CDS 35.0%
TATTCTCAAGTCATGCATCA+TGG - chr7_1:22188123-22188142 Msa1014160:intron 35.0%
ATGGGTGATAACCTAGTTTA+AGG + chr7_1:22187071-22187090 None:intergenic 35.0%
!! GTAGTTTGATCAGTTGATGA+GGG + chr7_1:22187094-22187113 None:intergenic 35.0%
TCATGAAGATGAAAAACCTC+AGG - chr7_1:22187355-22187374 Msa1014160:CDS 35.0%
CTAAATATAGTTCCAACCTG+AGG + chr7_1:22187374-22187393 None:intergenic 35.0%
TTTACTCTAGCATGTAGTTG+TGG - chr7_1:22187497-22187516 Msa1014160:intron 35.0%
!! ATTCAATCTAGCCTTGCATT+GGG + chr7_1:22187579-22187598 None:intergenic 35.0%
TAAAATAGCGTGCTTTGTCA+GGG + chr7_1:22187670-22187689 None:intergenic 35.0%
!!! TTTTGGTTCCTCGTTTGAAA+AGG - chr7_1:22187702-22187721 Msa1014160:CDS 35.0%
TTAGAACTCAATGATCAAGC+TGG - chr7_1:22187728-22187747 Msa1014160:CDS 35.0%
! GTTCAAGTAGTTGATGTAGT+TGG - chr7_1:22187794-22187813 Msa1014160:CDS 35.0%
!!! GGGAAAAAATTAGTTTTGGG+AGG - chr7_1:22187937-22187956 Msa1014160:CDS 35.0%
GCTTATGAGTGAAGTAAATC+AGG - chr7_1:22188010-22188029 Msa1014160:CDS 35.0%
TATTCTCAAGTCATGCATCA+TGG - chr7_1:22188123-22188142 Msa1014160:intron 35.0%
!! TTGATCAGTTGATGAGGGAT+GGG + chr7_1:22187089-22187108 None:intergenic 40.0%
!! TTTGATCAGTTGATGAGGGA+TGG + chr7_1:22187090-22187109 None:intergenic 40.0%
! GGTAGTTTGATCAGTTGATG+AGG + chr7_1:22187095-22187114 None:intergenic 40.0%
TGATGAAGAGGAAATCAGAG+AGG - chr7_1:22187292-22187311 Msa1014160:intron 40.0%
GATGAAGAGGAAATCAGAGA+GGG - chr7_1:22187293-22187312 Msa1014160:intron 40.0%
GAAGATGAAAAACCTCAGGT+TGG - chr7_1:22187359-22187378 Msa1014160:CDS 40.0%
AAGAGTTATGCCCAATCGAT+GGG - chr7_1:22187422-22187441 Msa1014160:intron 40.0%
! AACTCCAAAACCCATCGATT+GGG + chr7_1:22187435-22187454 None:intergenic 40.0%
TAACTCCAAAACCCATCGAT+TGG + chr7_1:22187436-22187455 None:intergenic 40.0%
!! CATTCAATCTAGCCTTGCAT+TGG + chr7_1:22187580-22187599 None:intergenic 40.0%
CTAAAATAGCGTGCTTTGTC+AGG + chr7_1:22187671-22187690 None:intergenic 40.0%
! TCTAACCTCCTTTTCAAACG+AGG + chr7_1:22187713-22187732 None:intergenic 40.0%
!! CGTGTTATTGATGACGACAA+CGG + chr7_1:22188285-22188304 None:intergenic 40.0%
!! TTGATCAGTTGATGAGGGAT+GGG + chr7_1:22187089-22187108 None:intergenic 40.0%
!! TTTGATCAGTTGATGAGGGA+TGG + chr7_1:22187090-22187109 None:intergenic 40.0%
! GGTAGTTTGATCAGTTGATG+AGG + chr7_1:22187095-22187114 None:intergenic 40.0%
TGATGAAGAGGAAATCAGAG+AGG - chr7_1:22187292-22187311 Msa1014160:intron 40.0%
GATGAAGAGGAAATCAGAGA+GGG - chr7_1:22187293-22187312 Msa1014160:intron 40.0%
GAAGATGAAAAACCTCAGGT+TGG - chr7_1:22187359-22187378 Msa1014160:CDS 40.0%
AAGAGTTATGCCCAATCGAT+GGG - chr7_1:22187422-22187441 Msa1014160:intron 40.0%
! AACTCCAAAACCCATCGATT+GGG + chr7_1:22187435-22187454 None:intergenic 40.0%
TAACTCCAAAACCCATCGAT+TGG + chr7_1:22187436-22187455 None:intergenic 40.0%
!! CATTCAATCTAGCCTTGCAT+TGG + chr7_1:22187580-22187599 None:intergenic 40.0%
CTAAAATAGCGTGCTTTGTC+AGG + chr7_1:22187671-22187690 None:intergenic 40.0%
! TCTAACCTCCTTTTCAAACG+AGG + chr7_1:22187713-22187732 None:intergenic 40.0%
!! CGTGTTATTGATGACGACAA+CGG + chr7_1:22188285-22188304 None:intergenic 40.0%
CAAGAGTTATGCCCAATCGA+TGG - chr7_1:22187421-22187440 Msa1014160:intron 45.0%
!! TATGCCCAATCGATGGGTTT+TGG - chr7_1:22187428-22187447 Msa1014160:intron 45.0%
TGGTTCCTCGTTTGAAAAGG+AGG - chr7_1:22187705-22187724 Msa1014160:CDS 45.0%
!!! AGTTTTGGGAGGAATGGACA+TGG - chr7_1:22187948-22187967 Msa1014160:CDS 45.0%
ACGACAACGGATCATTCACA+TGG + chr7_1:22188272-22188291 None:intergenic 45.0%
CAAGAGTTATGCCCAATCGA+TGG - chr7_1:22187421-22187440 Msa1014160:intron 45.0%
!! TATGCCCAATCGATGGGTTT+TGG - chr7_1:22187428-22187447 Msa1014160:intron 45.0%
TGGTTCCTCGTTTGAAAAGG+AGG - chr7_1:22187705-22187724 Msa1014160:CDS 45.0%
!!! AGTTTTGGGAGGAATGGACA+TGG - chr7_1:22187948-22187967 Msa1014160:CDS 45.0%
ACGACAACGGATCATTCACA+TGG + chr7_1:22188272-22188291 None:intergenic 45.0%
TTTGGGAGGAATGGACATGG+TGG - chr7_1:22187951-22187970 Msa1014160:CDS 50.0%
TTTGGGAGGAATGGACATGG+TGG - chr7_1:22187951-22187970 Msa1014160:CDS 50.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 22187034 22188314 22187034 ID=Msa1014160;Name=Msa1014160
chr7_1 mRNA 22187034 22188314 22187034 ID=Msa1014160-mRNA-1;Parent=Msa1014160;Name=Msa1014160-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|0.25|0|0.8|1|1|5|0|264
chr7_1 exon 22188293 22188314 22188293 ID=Msa1014160-mRNA-1:exon:5260;Parent=Msa1014160-mRNA-1
chr7_1 exon 22187513 22188078 22187513 ID=Msa1014160-mRNA-1:exon:5259;Parent=Msa1014160-mRNA-1
chr7_1 exon 22187317 22187412 22187317 ID=Msa1014160-mRNA-1:exon:5258;Parent=Msa1014160-mRNA-1
chr7_1 exon 22187178 22187234 22187178 ID=Msa1014160-mRNA-1:exon:5257;Parent=Msa1014160-mRNA-1
chr7_1 exon 22187034 22187087 22187034 ID=Msa1014160-mRNA-1:exon:5256;Parent=Msa1014160-mRNA-1
chr7_1 CDS 22188293 22188314 22188293 ID=Msa1014160-mRNA-1:cds;Parent=Msa1014160-mRNA-1
chr7_1 CDS 22187513 22188078 22187513 ID=Msa1014160-mRNA-1:cds;Parent=Msa1014160-mRNA-1
chr7_1 CDS 22187317 22187412 22187317 ID=Msa1014160-mRNA-1:cds;Parent=Msa1014160-mRNA-1
chr7_1 CDS 22187178 22187234 22187178 ID=Msa1014160-mRNA-1:cds;Parent=Msa1014160-mRNA-1
chr7_1 CDS 22187034 22187087 22187034 ID=Msa1014160-mRNA-1:cds;Parent=Msa1014160-mRNA-1
Gene Sequence

>Msa1014160

ATGGAAACAGTGCAAAAATCAATGGAAGAAGTATTTGTATCTTCTGATGAAGAGGAAATCAGAGAGGGAAATAATAATTTTGATGATAATTTAGAAGAAGATATTGTTCATGAAGATGAAAAACCTCAGGTTGGAACTATATTTAGCTCTAAAGATGAACTTTTTGAGTATTACAAGAGTTATGCCCAATCGATGGGTTTTGGAGTTACTAAACTTAGTAACAAAAATGCAGATGATGGGAAAAAATACTTTACTCTAGCATGTAGTTGTGGAACAAAGTATGTGAGTAAGTCGAAGAAGTTTTTGAACCCAAATCCTTTAACAAAAACCCAATGCAAGGCTAGATTGAATGCATGTATTTCTTTAGATGGAACAGTTACTATTGCAAAACTTTTTCTTGAGCATAATCATGATCTAATCCCTGACAAAGCACGCTATTTTAGAAGTAGTAAGTTTTTGGTTCCTCGTTTGAAAAGGAGGTTAGAACTCAATGATCAAGCTGGAATAAATGTACGCAGAAATTTTCGATCTAGAGTACAAGAGAGTGTTCAAGTAGTTGATGTAGTTGGCACACAAGAGAGTGTTCAAGGGGAAAAAATTAGTTTTGGGAGGAATGGACATGGTGGTGTAAATAATTTAGCTCCCTTCAATCCAAATTCAATGCTTATGAGTGAAGTAAATCAGGTTCCATTTTATTCTCAAGTCATGCATCATGGTGTTTCCTATTTTGAATTGTTACAGGCACAACACCATGTGAATGATCCGTTGTCGTCATCAATAACACGTGAATTATAA

Protein sequence

>Msa1014160

METVQKSMEEVFVSSDEEEIREGNNNFDDNLEEDIVHEDEKPQVGTIFSSKDELFEYYKSYAQSMGFGVTKLSNKNADDGKKYFTLACSCGTKYVSKSKKFLNPNPLTKTQCKARLNACISLDGTVTIAKLFLEHNHDLIPDKARYFRSSKFLVPRLKRRLELNDQAGINVRRNFRSRVQESVQVVDVVGTQESVQGEKISFGRNGHGGVNNLAPFNPNSMLMSEVNQVPFYSQVMHHGVSYFELLQAQHHVNDPLSSSITREL*