Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1019360 | AES79095.2 | 60.000 | 95 | 9 | 2 | 1 | 72 | 13 | 101 | 7.62e-22 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1019360 | G7L066 | 60.000 | 95 | 9 | 2 | 1 | 72 | 13 | 101 | 3.64e-22 | 93.2 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1019360 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1019360 | MtrunA17_Chr8g0351751 | 70.833 | 48 | 14 | 0 | 35 | 82 | 6 | 53 | 5.33e-15 | 67.0 |
| Msa1019360 | MtrunA17_Chr4g0014321 | 60.417 | 48 | 19 | 0 | 35 | 82 | 6 | 53 | 1.20e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCACATAAGGACCTTTAAA+AGG | 0.247852 | 7_1:+40975695 | None:intergenic |
| GAAGAAGGGTCTGTTGAATA+AGG | 0.356989 | 7_1:-40975430 | Msa1019360:CDS |
| ATAGTTACTCCTCCCACATA+AGG | 0.498334 | 7_1:+40975683 | None:intergenic |
| AACATACAAGAAAACGAAGA+AGG | 0.502252 | 7_1:-40975445 | Msa1019360:CDS |
| ATCTCACGTTATGGTTCACA+AGG | 0.544170 | 7_1:+40975354 | None:intergenic |
| TTTAAAGGTCCTTATGTGGG+AGG | 0.591138 | 7_1:-40975692 | Msa1019360:CDS |
| GGGAGGAGTAACTATGTTGG+TGG | 0.612331 | 7_1:-40975675 | Msa1019360:intron |
| ACATACAAGAAAACGAAGAA+GGG | 0.616612 | 7_1:-40975444 | Msa1019360:CDS |
| TTGGTTTATGCAGGGGAACG+AGG | 0.621187 | 7_1:-40975770 | None:intergenic |
| TGTGGGAGGAGTAACTATGT+TGG | 0.636800 | 7_1:-40975678 | Msa1019360:CDS |
| CACTAACTACAATTCTACAA+AGG | 0.644458 | 7_1:+40975391 | None:intergenic |
| AAGTTAAAGAGATTCTTGAA+CGG | 0.683075 | 7_1:-40975724 | Msa1019360:CDS |
| TCTCACGTTATGGTTCACAA+GGG | 0.748751 | 7_1:+40975355 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGTTAAAGAGATTCTTGAA+CGG | - | chr7_1:40975396-40975415 | Msa1019360:CDS | 25.0% |
| !!! | ATATTTTTGTGCCATAAGAA+TGG | - | chr7_1:40975489-40975508 | Msa1019360:CDS | 25.0% |
| ! | GAACTTTTCATCCATTCTTA+TGG | + | chr7_1:40975503-40975522 | None:intergenic | 30.0% |
| ! | TTTTACCTTTTCTCTAGTCA+TGG | + | chr7_1:40975620-40975639 | None:intergenic | 30.0% |
| AACATACAAGAAAACGAAGA+AGG | - | chr7_1:40975675-40975694 | Msa1019360:intron | 30.0% | |
| ACATACAAGAAAACGAAGAA+GGG | - | chr7_1:40975676-40975695 | Msa1019360:CDS | 30.0% | |
| CACTAACTACAATTCTACAA+AGG | + | chr7_1:40975732-40975751 | None:intergenic | 30.0% | |
| ! | GAACGGTGTAATCCTTTTAA+AGG | - | chr7_1:40975413-40975432 | Msa1019360:CDS | 35.0% |
| !! | TCCTTTTAAAGGTCCTTATG+TGG | - | chr7_1:40975424-40975443 | Msa1019360:CDS | 35.0% |
| !! | CCTTTTAAAGGTCCTTATGT+GGG | - | chr7_1:40975425-40975444 | Msa1019360:CDS | 35.0% |
| !!! | CATGGTTTTGACAAGAACAA+AGG | + | chr7_1:40975602-40975621 | None:intergenic | 35.0% |
| CAAAACCATGACTAGAGAAA+AGG | - | chr7_1:40975612-40975631 | Msa1019360:intron | 35.0% | |
| CCCACATAAGGACCTTTAAA+AGG | + | chr7_1:40975428-40975447 | None:intergenic | 40.0% | |
| TTTAAAGGTCCTTATGTGGG+AGG | - | chr7_1:40975428-40975447 | Msa1019360:CDS | 40.0% | |
| ATAGTTACTCCTCCCACATA+AGG | + | chr7_1:40975440-40975459 | None:intergenic | 40.0% | |
| GAAGAAGGGTCTGTTGAATA+AGG | - | chr7_1:40975690-40975709 | Msa1019360:CDS | 40.0% | |
| TGTGGGAGGAGTAACTATGT+TGG | - | chr7_1:40975442-40975461 | Msa1019360:CDS | 45.0% | |
| GGGAGGAGTAACTATGTTGG+TGG | - | chr7_1:40975445-40975464 | Msa1019360:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 40975365 | 40975777 | 40975365 | ID=Msa1019360;Name=Msa1019360 |
| chr7_1 | mRNA | 40975365 | 40975777 | 40975365 | ID=Msa1019360-mRNA-1;Parent=Msa1019360;Name=Msa1019360-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|0|0|2|0|82 |
| chr7_1 | exon | 40975676 | 40975777 | 40975676 | ID=Msa1019360-mRNA-1:exon:7968;Parent=Msa1019360-mRNA-1 |
| chr7_1 | exon | 40975365 | 40975508 | 40975365 | ID=Msa1019360-mRNA-1:exon:7967;Parent=Msa1019360-mRNA-1 |
| chr7_1 | CDS | 40975676 | 40975777 | 40975676 | ID=Msa1019360-mRNA-1:cds;Parent=Msa1019360-mRNA-1 |
| chr7_1 | CDS | 40975365 | 40975508 | 40975365 | ID=Msa1019360-mRNA-1:cds;Parent=Msa1019360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
