Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023750 | KEH17097.1 | 80.000 | 60 | 12 | 0 | 1 | 60 | 1 | 60 | 2.25e-27 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023750 | G7ZUP9 | 80.000 | 60 | 12 | 0 | 1 | 60 | 1 | 60 | 1.07e-27 | 107 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1023750 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGCTTTGAACGTCCATAAA+AGG | 0.292733 | 7_1:+51913158 | Msa1023750:CDS |
| ATATACTATAAACTGAAATA+TGG | 0.301575 | 7_1:-51913343 | None:intergenic |
| TGTTCTCCAGTATATCTTCT+TGG | 0.311912 | 7_1:-51913318 | None:intergenic |
| AATTCTCCAAGAAGATATAC+TGG | 0.369151 | 7_1:+51913312 | Msa1023750:CDS |
| TCCCAAGTCCTTCGAAAGTA+GGG | 0.449688 | 7_1:+51913135 | Msa1023750:CDS |
| GTGGTTGACATGTCTTCATA+TGG | 0.455172 | 7_1:-51913252 | None:intergenic |
| ACCCCTACTTTCGAAGGACT+TGG | 0.483526 | 7_1:-51913137 | None:intergenic |
| CCCCTACTTTCGAAGGACTT+GGG | 0.544991 | 7_1:-51913136 | None:intergenic |
| GTCCCAAGTCCTTCGAAAGT+AGG | 0.556450 | 7_1:+51913134 | Msa1023750:CDS |
| TTATGTACTTGCGGCTTCTG+TGG | 0.572456 | 7_1:-51913271 | None:intergenic |
| CAAAGCACCCCTACTTTCGA+AGG | 0.573103 | 7_1:-51913143 | None:intergenic |
| CCCAAGTCCTTCGAAAGTAG+GGG | 0.658544 | 7_1:+51913136 | Msa1023750:CDS |
| TGGGACAATCAAAGCAAGCG+TGG | 0.684170 | 7_1:-51913117 | None:intergenic |
| AAAACACGTTGCGATAGATG+TGG | 0.685090 | 7_1:+51913196 | Msa1023750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATACTATAAACTGAAATA+TGG | - | chr7_1:51913346-51913365 | None:intergenic | 15.0% |
| ! | AGAATTCTTCAAATTCATTG+AGG | - | chr7_1:51913298-51913317 | None:intergenic | 25.0% |
| ! | CTTCACATTCAAACCTTTTA+TGG | - | chr7_1:51913174-51913193 | None:intergenic | 30.0% |
| AATTCTCCAAGAAGATATAC+TGG | + | chr7_1:51913312-51913331 | Msa1023750:CDS | 30.0% | |
| CATTGAGGATTATGTACTTG+CGG | - | chr7_1:51913283-51913302 | None:intergenic | 35.0% | |
| TGTTCTCCAGTATATCTTCT+TGG | - | chr7_1:51913321-51913340 | None:intergenic | 35.0% | |
| ! | GTGCTTTGAACGTCCATAAA+AGG | + | chr7_1:51913158-51913177 | Msa1023750:CDS | 40.0% |
| AAAACACGTTGCGATAGATG+TGG | + | chr7_1:51913196-51913215 | Msa1023750:CDS | 40.0% | |
| GTGGTTGACATGTCTTCATA+TGG | - | chr7_1:51913255-51913274 | None:intergenic | 40.0% | |
| TCCCAAGTCCTTCGAAAGTA+GGG | + | chr7_1:51913135-51913154 | Msa1023750:CDS | 45.0% | |
| TTATGTACTTGCGGCTTCTG+TGG | - | chr7_1:51913274-51913293 | None:intergenic | 45.0% | |
| TGGGACAATCAAAGCAAGCG+TGG | - | chr7_1:51913120-51913139 | None:intergenic | 50.0% | |
| GTCCCAAGTCCTTCGAAAGT+AGG | + | chr7_1:51913134-51913153 | Msa1023750:CDS | 50.0% | |
| CCCCTACTTTCGAAGGACTT+GGG | - | chr7_1:51913139-51913158 | None:intergenic | 50.0% | |
| CCCAAGTCCTTCGAAAGTAG+GGG | + | chr7_1:51913136-51913155 | Msa1023750:CDS | 50.0% | |
| ACCCCTACTTTCGAAGGACT+TGG | - | chr7_1:51913140-51913159 | None:intergenic | 50.0% | |
| ! | CAAAGCACCCCTACTTTCGA+AGG | - | chr7_1:51913146-51913165 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 51913112 | 51913413 | 51913112 | ID=Msa1023750;Name=Msa1023750 |
| chr7_1 | mRNA | 51913112 | 51913413 | 51913112 | ID=Msa1023750-mRNA-1;Parent=Msa1023750;Name=Msa1023750-mRNA-1;_AED=0.43;_eAED=0.63;_QI=0|0|0|1|0|0|2|0|91 |
| chr7_1 | exon | 51913112 | 51913285 | 51913112 | ID=Msa1023750-mRNA-1:exon:10250;Parent=Msa1023750-mRNA-1 |
| chr7_1 | exon | 51913312 | 51913413 | 51913312 | ID=Msa1023750-mRNA-1:exon:10251;Parent=Msa1023750-mRNA-1 |
| chr7_1 | CDS | 51913112 | 51913285 | 51913112 | ID=Msa1023750-mRNA-1:cds;Parent=Msa1023750-mRNA-1 |
| chr7_1 | CDS | 51913312 | 51913413 | 51913312 | ID=Msa1023750-mRNA-1:cds;Parent=Msa1023750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
