Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023760 | KEH17097.1 | 80.000 | 60 | 12 | 0 | 1 | 60 | 1 | 60 | 2.25e-27 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023760 | G7ZUP9 | 80.000 | 60 | 12 | 0 | 1 | 60 | 1 | 60 | 1.07e-27 | 107 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1023760 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGCTTTGAACGTCCATAAA+AGG | 0.292733 | 7_1:+51915353 | Msa1023760:CDS |
| ATATACTATAAACTGAAATA+TGG | 0.301575 | 7_1:-51915538 | None:intergenic |
| TGTTCTCCAGTATATCTTCT+TGG | 0.311912 | 7_1:-51915513 | None:intergenic |
| AATTCTCCAAGAAGATATAC+TGG | 0.369151 | 7_1:+51915507 | Msa1023760:CDS |
| TCCCAAGTCCTTCGAAAGTA+GGG | 0.449688 | 7_1:+51915330 | Msa1023760:CDS |
| GTGGTTGACATGTCTTCATA+TGG | 0.455172 | 7_1:-51915447 | None:intergenic |
| ACCCCTACTTTCGAAGGACT+TGG | 0.483526 | 7_1:-51915332 | None:intergenic |
| CCCCTACTTTCGAAGGACTT+GGG | 0.544991 | 7_1:-51915331 | None:intergenic |
| GTCCCAAGTCCTTCGAAAGT+AGG | 0.556450 | 7_1:+51915329 | Msa1023760:CDS |
| TTATGTACTTGCGGCTTCTG+TGG | 0.572456 | 7_1:-51915466 | None:intergenic |
| CAAAGCACCCCTACTTTCGA+AGG | 0.573103 | 7_1:-51915338 | None:intergenic |
| CCCAAGTCCTTCGAAAGTAG+GGG | 0.658544 | 7_1:+51915331 | Msa1023760:CDS |
| TGGGACAATCAAAGCAAGCG+TGG | 0.684170 | 7_1:-51915312 | None:intergenic |
| AAAACACGTTGCGATAGATG+TGG | 0.685090 | 7_1:+51915391 | Msa1023760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATACTATAAACTGAAATA+TGG | - | chr7_1:51915541-51915560 | None:intergenic | 15.0% |
| ! | AGAATTCTTCAAATTCATTG+AGG | - | chr7_1:51915493-51915512 | None:intergenic | 25.0% |
| ! | CTTCACATTCAAACCTTTTA+TGG | - | chr7_1:51915369-51915388 | None:intergenic | 30.0% |
| AATTCTCCAAGAAGATATAC+TGG | + | chr7_1:51915507-51915526 | Msa1023760:CDS | 30.0% | |
| CATTGAGGATTATGTACTTG+CGG | - | chr7_1:51915478-51915497 | None:intergenic | 35.0% | |
| TGTTCTCCAGTATATCTTCT+TGG | - | chr7_1:51915516-51915535 | None:intergenic | 35.0% | |
| ! | GTGCTTTGAACGTCCATAAA+AGG | + | chr7_1:51915353-51915372 | Msa1023760:CDS | 40.0% |
| AAAACACGTTGCGATAGATG+TGG | + | chr7_1:51915391-51915410 | Msa1023760:CDS | 40.0% | |
| GTGGTTGACATGTCTTCATA+TGG | - | chr7_1:51915450-51915469 | None:intergenic | 40.0% | |
| TCCCAAGTCCTTCGAAAGTA+GGG | + | chr7_1:51915330-51915349 | Msa1023760:CDS | 45.0% | |
| TTATGTACTTGCGGCTTCTG+TGG | - | chr7_1:51915469-51915488 | None:intergenic | 45.0% | |
| TGGGACAATCAAAGCAAGCG+TGG | - | chr7_1:51915315-51915334 | None:intergenic | 50.0% | |
| GTCCCAAGTCCTTCGAAAGT+AGG | + | chr7_1:51915329-51915348 | Msa1023760:CDS | 50.0% | |
| CCCCTACTTTCGAAGGACTT+GGG | - | chr7_1:51915334-51915353 | None:intergenic | 50.0% | |
| CCCAAGTCCTTCGAAAGTAG+GGG | + | chr7_1:51915331-51915350 | Msa1023760:CDS | 50.0% | |
| ACCCCTACTTTCGAAGGACT+TGG | - | chr7_1:51915335-51915354 | None:intergenic | 50.0% | |
| ! | CAAAGCACCCCTACTTTCGA+AGG | - | chr7_1:51915341-51915360 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 51915307 | 51915608 | 51915307 | ID=Msa1023760;Name=Msa1023760 |
| chr7_1 | mRNA | 51915307 | 51915608 | 51915307 | ID=Msa1023760-mRNA-1;Parent=Msa1023760;Name=Msa1023760-mRNA-1;_AED=0.43;_eAED=0.63;_QI=0|0|0|1|0|0|2|0|91 |
| chr7_1 | exon | 51915307 | 51915480 | 51915307 | ID=Msa1023760-mRNA-1:exon:10254;Parent=Msa1023760-mRNA-1 |
| chr7_1 | exon | 51915507 | 51915608 | 51915507 | ID=Msa1023760-mRNA-1:exon:10255;Parent=Msa1023760-mRNA-1 |
| chr7_1 | CDS | 51915307 | 51915480 | 51915307 | ID=Msa1023760-mRNA-1:cds;Parent=Msa1023760-mRNA-1 |
| chr7_1 | CDS | 51915507 | 51915608 | 51915507 | ID=Msa1023760-mRNA-1:cds;Parent=Msa1023760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
