Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023770 | KEH17097.1 | 83.333 | 36 | 6 | 0 | 1 | 36 | 1 | 36 | 3.20e-12 | 68.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1023770 | G7ZUP9 | 83.333 | 36 | 6 | 0 | 1 | 36 | 1 | 36 | 1.53e-12 | 68.9 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1023770 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGTCCTTGAAGCTTCTA+TGG | 0.208414 | 7_1:+51923453 | None:intergenic |
| GTGCTTTGAACGTCCATAAA+AGG | 0.292733 | 7_1:-51923951 | Msa1023770:CDS |
| TGATCACCTCTTGCATGATT+TGG | 0.298179 | 7_1:+51923484 | None:intergenic |
| AAGGATTGAACAAGGATTCT+TGG | 0.320931 | 7_1:-51923417 | Msa1023770:CDS |
| CAAGAACCATAGAAGCTTCA+AGG | 0.445019 | 7_1:-51923459 | Msa1023770:CDS |
| TCCCAAGTCCTTCGAAAGTA+GGG | 0.449688 | 7_1:-51923974 | Msa1023770:CDS |
| TGAACAAGGATTCTTGGCCT+AGG | 0.463724 | 7_1:-51923411 | Msa1023770:CDS |
| TCTCCTCCTTCTCTCTAAGA+TGG | 0.479787 | 7_1:-51923542 | Msa1023770:CDS |
| ACAAGAAACAACACTGAGAA+AGG | 0.481337 | 7_1:-51923436 | Msa1023770:CDS |
| ACCCCTACTTTCGAAGGACT+TGG | 0.483526 | 7_1:+51923972 | None:intergenic |
| GAACAAGGATTCTTGGCCTA+GGG | 0.522869 | 7_1:-51923410 | Msa1023770:CDS |
| TTGGAAAGCCTAGGATTGAT+CGG | 0.533594 | 7_1:+51923503 | None:intergenic |
| CCCCTACTTTCGAAGGACTT+GGG | 0.544991 | 7_1:+51923973 | None:intergenic |
| TTCCCTCTCCGATCAATCCT+AGG | 0.547053 | 7_1:-51923511 | Msa1023770:CDS |
| AGGATTGATCGGAGAGGGAA+GGG | 0.548590 | 7_1:+51923514 | None:intergenic |
| GTCCCAAGTCCTTCGAAAGT+AGG | 0.556450 | 7_1:-51923975 | Msa1023770:CDS |
| TTGCATGATTTGGAAAGCCT+AGG | 0.562308 | 7_1:+51923494 | None:intergenic |
| CAAAGCACCCCTACTTTCGA+AGG | 0.573103 | 7_1:+51923966 | None:intergenic |
| AGCCTAGGATTGATCGGAGA+GGG | 0.573131 | 7_1:+51923509 | None:intergenic |
| TAGGATTGATCGGAGAGGGA+AGG | 0.584318 | 7_1:+51923513 | None:intergenic |
| GCACCATCTTAGAGAGAAGG+AGG | 0.588986 | 7_1:+51923539 | None:intergenic |
| GTGGCACCATCTTAGAGAGA+AGG | 0.589764 | 7_1:+51923536 | None:intergenic |
| GGCTTTCCAAATCATGCAAG+AGG | 0.598475 | 7_1:-51923490 | Msa1023770:CDS |
| CACTGAGAAAGGATTGAACA+AGG | 0.609104 | 7_1:-51923425 | Msa1023770:CDS |
| ATTGATCGGAGAGGGAAGGG+TGG | 0.650282 | 7_1:+51923517 | None:intergenic |
| CCCAAGTCCTTCGAAAGTAG+GGG | 0.658544 | 7_1:-51923973 | Msa1023770:CDS |
| TGGGACAATCAAAGCAAGCG+TGG | 0.684170 | 7_1:+51923992 | None:intergenic |
| AAAACACGTTGCGATAGATG+TGG | 0.685090 | 7_1:-51923913 | Msa1023770:intron |
| AAGCCTAGGATTGATCGGAG+AGG | 0.706988 | 7_1:+51923508 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTGGAAAAATGAATAATTT+TGG | + | chr7_1:51923804-51923823 | None:intergenic | 20.0% |
| ! | CTTCACATTCAAACCTTTTA+TGG | + | chr7_1:51923467-51923486 | None:intergenic | 30.0% |
| TTATCTGTTAAATGTGTCAG+AGG | - | chr7_1:51923610-51923629 | Msa1023770:intron | 30.0% | |
| !!! | ACTAATGTGAAGGGTATTTT+GGG | - | chr7_1:51923742-51923761 | Msa1023770:intron | 30.0% |
| !! | AAGGGAGTATATAAGTGTTT+TGG | - | chr7_1:51923776-51923795 | Msa1023770:intron | 30.0% |
| !! | AGGGAGTATATAAGTGTTTT+GGG | - | chr7_1:51923777-51923796 | Msa1023770:intron | 30.0% |
| AATTTCCATTAGCTGACGAA+CGG | + | chr7_1:51923590-51923609 | None:intergenic | 35.0% | |
| TGACGAAAATGTTAAGTACG+AGG | - | chr7_1:51923657-51923676 | Msa1023770:intron | 35.0% | |
| GACGAAAATGTTAAGTACGA+GGG | - | chr7_1:51923658-51923677 | Msa1023770:intron | 35.0% | |
| TAGGTGATATCAGTCAAGTA+TGG | - | chr7_1:51923691-51923710 | Msa1023770:intron | 35.0% | |
| !!! | GACTAATGTGAAGGGTATTT+TGG | - | chr7_1:51923741-51923760 | Msa1023770:intron | 35.0% |
| !! | GGGAGTATATAAGTGTTTTG+GGG | - | chr7_1:51923778-51923797 | Msa1023770:intron | 35.0% |
| ACAAGAAACAACACTGAGAA+AGG | - | chr7_1:51923966-51923985 | Msa1023770:CDS | 35.0% | |
| AAGGATTGAACAAGGATTCT+TGG | - | chr7_1:51923985-51924004 | Msa1023770:CDS | 35.0% | |
| ! | GTGCTTTGAACGTCCATAAA+AGG | - | chr7_1:51923451-51923470 | Msa1023770:CDS | 40.0% |
| AAAACACGTTGCGATAGATG+TGG | - | chr7_1:51923489-51923508 | Msa1023770:CDS | 40.0% | |
| GTGGTTGACATGTCTTCATA+TGG | + | chr7_1:51923548-51923567 | None:intergenic | 40.0% | |
| ATTTCCATTAGCTGACGAAC+GGG | + | chr7_1:51923589-51923608 | None:intergenic | 40.0% | |
| TGTCAGAGGACTAAGATTCA+TGG | - | chr7_1:51923624-51923643 | Msa1023770:intron | 40.0% | |
| GTACGAGGGAAGCTAATATT+AGG | - | chr7_1:51923672-51923691 | Msa1023770:intron | 40.0% | |
| GTAAACGCGACTAATGTGAA+GGG | - | chr7_1:51923733-51923752 | Msa1023770:intron | 40.0% | |
| !! | ATTTTGGGTAATTGCTCGCA+AGG | - | chr7_1:51923757-51923776 | Msa1023770:intron | 40.0% |
| !! | TTTTGGGTAATTGCTCGCAA+GGG | - | chr7_1:51923758-51923777 | Msa1023770:intron | 40.0% |
| TTGGAAAGCCTAGGATTGAT+CGG | + | chr7_1:51923902-51923921 | None:intergenic | 40.0% | |
| ! | TTGCATGATTTGGAAAGCCT+AGG | + | chr7_1:51923911-51923930 | None:intergenic | 40.0% |
| TGATCACCTCTTGCATGATT+TGG | + | chr7_1:51923921-51923940 | None:intergenic | 40.0% | |
| CAAGAACCATAGAAGCTTCA+AGG | - | chr7_1:51923943-51923962 | Msa1023770:CDS | 40.0% | |
| TCTTGTCCTTGAAGCTTCTA+TGG | + | chr7_1:51923952-51923971 | None:intergenic | 40.0% | |
| CACTGAGAAAGGATTGAACA+AGG | - | chr7_1:51923977-51923996 | Msa1023770:CDS | 40.0% | |
| TCCCAAGTCCTTCGAAAGTA+GGG | - | chr7_1:51923428-51923447 | Msa1023770:CDS | 45.0% | |
| GATATTACATGCGGCTTCTG+TGG | + | chr7_1:51923567-51923586 | None:intergenic | 45.0% | |
| GACGAACGGGATATTACATG+CGG | + | chr7_1:51923576-51923595 | None:intergenic | 45.0% | |
| ATATCCCGTTCGTCAGCTAA+TGG | - | chr7_1:51923582-51923601 | Msa1023770:intron | 45.0% | |
| TGGACTTATGTATCAGAGCC+AGG | - | chr7_1:51923711-51923730 | Msa1023770:intron | 45.0% | |
| TCACATTAGTCGCGTTTACC+TGG | + | chr7_1:51923732-51923751 | None:intergenic | 45.0% | |
| GGTAAACGCGACTAATGTGA+AGG | - | chr7_1:51923732-51923751 | Msa1023770:intron | 45.0% | |
| CCTAGAGAGAGAAAGAGAAG+TGG | + | chr7_1:51923822-51923841 | None:intergenic | 45.0% | |
| CCACTTCTCTTTCTCTCTCT+AGG | - | chr7_1:51923819-51923838 | Msa1023770:intron | 45.0% | |
| ! | TCTCCTCCTTCTCTCTAAGA+TGG | - | chr7_1:51923860-51923879 | Msa1023770:intron | 45.0% |
| GGCTTTCCAAATCATGCAAG+AGG | - | chr7_1:51923912-51923931 | Msa1023770:intron | 45.0% | |
| TGAACAAGGATTCTTGGCCT+AGG | - | chr7_1:51923991-51924010 | Msa1023770:CDS | 45.0% | |
| GAACAAGGATTCTTGGCCTA+GGG | - | chr7_1:51923992-51924011 | Msa1023770:CDS | 45.0% | |
| TGGGACAATCAAAGCAAGCG+TGG | + | chr7_1:51923413-51923432 | None:intergenic | 50.0% | |
| GTCCCAAGTCCTTCGAAAGT+AGG | - | chr7_1:51923427-51923446 | Msa1023770:CDS | 50.0% | |
| CCCCTACTTTCGAAGGACTT+GGG | + | chr7_1:51923432-51923451 | None:intergenic | 50.0% | |
| CCCAAGTCCTTCGAAAGTAG+GGG | - | chr7_1:51923429-51923448 | Msa1023770:CDS | 50.0% | |
| ACCCCTACTTTCGAAGGACT+TGG | + | chr7_1:51923433-51923452 | None:intergenic | 50.0% | |
| ! | CAAAGCACCCCTACTTTCGA+AGG | + | chr7_1:51923439-51923458 | None:intergenic | 50.0% |
| GCACCATCTTAGAGAGAAGG+AGG | + | chr7_1:51923866-51923885 | None:intergenic | 50.0% | |
| GTGGCACCATCTTAGAGAGA+AGG | + | chr7_1:51923869-51923888 | None:intergenic | 50.0% | |
| AGGATTGATCGGAGAGGGAA+GGG | + | chr7_1:51923891-51923910 | None:intergenic | 50.0% | |
| TAGGATTGATCGGAGAGGGA+AGG | + | chr7_1:51923892-51923911 | None:intergenic | 50.0% | |
| TTCCCTCTCCGATCAATCCT+AGG | - | chr7_1:51923891-51923910 | Msa1023770:intron | 50.0% | |
| AGCCTAGGATTGATCGGAGA+GGG | + | chr7_1:51923896-51923915 | None:intergenic | 50.0% | |
| AAGCCTAGGATTGATCGGAG+AGG | + | chr7_1:51923897-51923916 | None:intergenic | 50.0% | |
| ATTGATCGGAGAGGGAAGGG+TGG | + | chr7_1:51923888-51923907 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 51923405 | 51924019 | 51923405 | ID=Msa1023770;Name=Msa1023770 |
| chr7_1 | mRNA | 51923405 | 51924019 | 51923405 | ID=Msa1023770-mRNA-1;Parent=Msa1023770;Name=Msa1023770-mRNA-1;_AED=0.76;_eAED=0.76;_QI=0|0|0|0.5|1|1|2|0|94 |
| chr7_1 | exon | 51923914 | 51924019 | 51923914 | ID=Msa1023770-mRNA-1:exon:10239;Parent=Msa1023770-mRNA-1 |
| chr7_1 | exon | 51923405 | 51923583 | 51923405 | ID=Msa1023770-mRNA-1:exon:10238;Parent=Msa1023770-mRNA-1 |
| chr7_1 | CDS | 51923914 | 51924019 | 51923914 | ID=Msa1023770-mRNA-1:cds;Parent=Msa1023770-mRNA-1 |
| chr7_1 | CDS | 51923405 | 51923583 | 51923405 | ID=Msa1023770-mRNA-1:cds;Parent=Msa1023770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
