AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1026330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1026330 MtrunA17_Chr2g0300441 86.017 236 33 0 1 236 1 236 2.98e-150 417
Msa1026330 MtrunA17_Chr7g0238991 83.784 222 35 1 16 236 27 248 4.12e-137 385
Msa1026330 MtrunA17_Chr8g0355091 71.730 237 66 1 1 236 1 237 1.04e-121 345
Msa1026330 MtrunA17_Chr1g0206721 73.973 219 57 0 1 219 1 219 9.59e-117 333
Msa1026330 MtrunA17_Chr1g0206701 72.000 225 63 0 1 225 1 225 5.12e-116 331
Msa1026330 MtrunA17_Chr2g0292791 73.059 219 59 0 1 219 1 219 9.12e-115 328
Msa1026330 MtrunA17_Chr1g0149391 72.146 219 61 0 1 219 1 219 1.30e-112 322
Msa1026330 MtrunA17_Chr1g0208561 68.511 235 56 3 1 225 1 227 4.59e-111 318
Msa1026330 MtrunA17_Chr3g0089141 68.511 235 56 3 1 225 1 227 1.38e-109 314
Msa1026330 MtrunA17_Chr2g0325971 68.936 235 55 3 1 225 1 227 3.91e-109 313
Msa1026330 MtrunA17_Chr3g0099421 70.320 219 65 0 1 219 26 244 5.57e-109 314
Msa1026330 MtrunA17_Chr1g0208581 68.220 236 55 3 1 225 1 227 8.72e-107 307
Msa1026330 MtrunA17_Chr2g0317451 63.136 236 86 1 1 235 1 236 7.15e-104 300
Msa1026330 MtrunA17_Chr2g0317481 63.136 236 86 1 1 235 1 236 2.52e-103 299
Msa1026330 MtrunA17_Chr1g0187361 63.556 225 82 0 1 225 1 225 3.82e-101 293
Msa1026330 MtrunA17_Chr1g0206691 65.000 220 76 1 18 236 1 220 6.11e-101 292
Msa1026330 MtrunA17_Chr5g0422941 61.181 237 91 1 1 236 1 237 1.26e-100 292
Msa1026330 MtrunA17_Chr1g0188671 62.222 225 85 0 1 225 39 263 6.44e-99 289
Msa1026330 MtrunA17_Chr5g0422421 63.556 225 82 0 1 225 1 225 5.58e-97 283
Msa1026330 MtrunA17_Chr5g0419601 61.778 225 86 0 1 225 1 225 7.45e-96 280
Msa1026330 MtrunA17_Chr3g0099411 63.927 219 60 1 1 219 1 200 1.09e-93 273
Msa1026330 MtrunA17_Chr1g0206991 72.376 181 50 0 1 181 1 181 1.18e-92 270
Msa1026330 MtrunA17_Chr3g0089151 82.576 132 23 0 1 132 1 132 1.22e-74 222
Msa1026330 MtrunA17_Chr2g0300421 71.642 134 38 0 1 134 1 134 2.71e-62 192
Msa1026330 MtrunA17_Chr3g0085241 44.949 198 100 1 3 191 35 232 3.10e-54 174
Msa1026330 MtrunA17_Chr3g0119901 52.632 152 72 0 9 160 9 160 1.93e-53 171
Msa1026330 MtrunA17_Chr3g0111931 63.725 102 36 1 21 121 2 103 2.62e-39 131
Msa1026330 MtrunA17_Chr8g0379021 38.462 182 96 3 5 183 8 176 4.49e-34 120
Msa1026330 MtrunA17_Chr2g0333981 38.037 163 97 1 5 167 8 166 1.17e-32 117
Msa1026330 MtrunA17_Chr1g0187351 80.303 66 13 0 18 83 1 66 5.25e-32 112
Msa1026330 MtrunA17_Chr3g0111841 83.051 59 10 0 15 73 2 60 1.60e-28 103
Msa1026330 MtrunA17_Chr4g0018951 42.754 138 67 3 22 159 25 150 6.76e-27 101
Msa1026330 MtrunA17_Chr4g0071601 32.927 164 96 3 22 175 25 184 5.46e-23 92.0
Msa1026330 MtrunA17_Chr1g0175601 44.554 101 54 1 9 109 2 100 2.53e-22 90.1
Msa1026330 MtrunA17_Chr1g0175611 52.564 78 37 0 9 86 2 79 8.87e-22 88.6
Msa1026330 MtrunA17_Chr1g0169761 47.778 90 38 1 9 89 2 91 1.53e-21 88.2
Msa1026330 MtrunA17_Chr4g0074101 37.600 125 63 2 9 118 2 126 2.22e-21 88.2
Msa1026330 MtrunA17_Chr8g0376821 55.072 69 31 0 9 77 2 70 3.98e-21 87.0
Msa1026330 MtrunA17_Chr1g0180591 42.574 101 56 1 9 109 2 100 9.48e-21 85.9
Msa1026330 MtrunA17_Chr3g0111831 66.102 59 20 0 16 74 11 69 9.73e-21 83.2
Msa1026330 MtrunA17_Chr8g0357771 53.623 69 32 0 9 77 2 70 1.24e-19 83.2
Msa1026330 MtrunA17_Chr8g0356071 52.174 69 33 0 9 77 2 70 1.01e-18 80.1
Msa1026330 MtrunA17_Chr4g0052061 37.273 110 67 1 9 118 2 109 2.64e-17 76.3
Msa1026330 MtrunA17_Chr4g0049901 32.353 204 102 6 11 186 30 225 3.56e-17 77.8
Msa1026330 MtrunA17_Chr3g0077501 48.529 68 35 0 9 76 2 69 2.49e-16 75.1
Msa1026330 MtrunA17_Chr8g0351041 30.000 170 103 3 12 177 5 162 4.97e-16 73.2
Msa1026330 MtrunA17_Chr4g0057151 50.769 65 32 0 12 76 5 69 6.02e-16 73.9
Msa1026330 MtrunA17_Chr4g0052081 36.792 106 66 1 9 113 2 107 6.88e-16 73.2
Msa1026330 MtrunA17_Chr5g0412461 48.485 66 34 0 11 76 4 69 7.56e-16 73.9
Msa1026330 MtrunA17_Chr3g0097101 40.476 84 50 0 3 86 11 94 9.57e-16 73.9
Msa1026330 MtrunA17_Chr3g0134451 43.137 102 55 3 9 109 2 101 1.01e-15 75.1
Msa1026330 MtrunA17_Chr5g0412271 48.485 66 34 0 11 76 4 69 1.15e-15 73.6
Msa1026330 MtrunA17_Chr8g0351051 47.059 68 36 0 9 76 2 69 4.76e-15 72.0
Msa1026330 MtrunA17_Chr8g0380021 38.776 98 53 2 9 100 18 114 7.77e-15 71.6
Msa1026330 MtrunA17_Chr8g0351031 43.902 82 45 1 12 92 5 86 1.03e-14 70.9
Msa1026330 MtrunA17_Chr5g0427401 45.455 66 36 0 11 76 4 69 1.04e-14 70.9
Msa1026330 MtrunA17_Chr5g0425191 56.604 53 23 0 9 61 2 54 1.31e-14 67.8
Msa1026330 MtrunA17_Chr6g0474371 44.928 69 37 1 9 76 2 70 2.15e-14 69.3
Msa1026330 MtrunA17_Chr2g0284911 47.059 68 36 0 9 76 17 84 2.63e-14 70.1
Msa1026330 MtrunA17_Chr8g0345001 32.418 182 103 6 2 177 13 180 2.82e-14 68.9
Msa1026330 MtrunA17_Chr4g0020221 40.244 82 49 0 9 90 2 83 2.91e-14 70.1
Msa1026330 MtrunA17_Chr7g0246431 47.692 65 34 0 12 76 5 69 3.47e-14 69.3
Msa1026330 MtrunA17_Chr2g0279821 44.118 68 38 0 9 76 2 69 3.73e-14 69.7
Msa1026330 MtrunA17_Chr5g0420081 37.391 115 53 5 9 111 2 109 4.49e-14 69.3
Msa1026330 MtrunA17_Chr4g0061691 43.478 69 38 1 9 76 2 70 6.06e-14 68.9
Msa1026330 MtrunA17_Chr4g0044071 52.632 57 27 0 9 65 2 58 6.09e-14 66.2
Msa1026330 MtrunA17_Chr7g0220121 34.483 116 65 4 9 114 2 116 7.96e-14 68.9
Msa1026330 MtrunA17_Chr1g0173161 49.180 61 31 0 9 69 2 62 1.00e-13 64.7
Msa1026330 MtrunA17_Chr1g0204111 45.588 68 37 0 9 76 18 85 2.39e-13 67.4
Msa1026330 MtrunA17_Chr6g0455511 44.928 69 37 1 9 76 2 70 2.62e-13 67.4
Msa1026330 MtrunA17_Chr7g0220111 44.928 69 37 1 9 76 2 70 3.42e-13 67.0
Msa1026330 MtrunA17_Chr4g0049541 42.647 68 39 0 9 76 2 69 3.66e-13 66.6
Msa1026330 MtrunA17_Chr2g0308251 51.786 56 27 0 9 64 2 57 4.44e-13 63.2
Msa1026330 MtrunA17_Chr8g0366231 42.029 69 39 1 9 76 2 70 4.66e-13 66.6
Msa1026330 MtrunA17_Chr3g0142191 48.333 60 31 0 12 71 5 64 5.00e-13 66.2
Msa1026330 MtrunA17_Chr5g0411691 41.176 68 40 0 9 76 2 69 7.13e-13 65.9
Msa1026330 MtrunA17_Chr1g0164791 44.286 70 37 1 9 76 2 71 7.14e-13 65.9
Msa1026330 MtrunA17_Chr7g0246381 45.455 66 31 1 9 69 2 67 8.16e-13 62.0
Msa1026330 MtrunA17_Chr5g0427921 43.860 57 32 0 9 65 2 58 9.21e-13 61.6
Msa1026330 MtrunA17_Chr3g0093131 34.965 143 76 4 26 159 36 170 1.09e-12 64.7
Msa1026330 MtrunA17_Chr8g0377561 50.000 56 28 0 9 64 2 57 1.24e-12 62.4
Msa1026330 MtrunA17_Chr1g0159661 45.455 66 35 1 9 74 2 66 1.73e-12 63.9
Msa1026330 MtrunA17_Chr5g0404951 45.714 70 36 1 9 76 2 71 1.81e-12 64.7
Msa1026330 MtrunA17_Chr5g0420091 50.000 56 28 0 9 64 2 57 2.37e-12 64.3
Msa1026330 MtrunA17_Chr3g0122251 51.786 56 27 0 9 64 2 57 2.56e-12 64.7
Msa1026330 MtrunA17_Chr4g0061671 47.368 57 30 0 9 65 2 58 3.10e-12 63.2
Msa1026330 MtrunA17_Chr3g0125241 45.455 66 35 1 9 74 27 91 5.28e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1026330 AT5G60440.1 53.293 167 75 2 5 168 3 169 3.73e-52 171
Msa1026330 AT2G24840.1 45.000 160 79 3 9 163 63 218 3.65e-36 129
Msa1026330 AT1G01530.1 43.902 164 89 2 4 166 2 163 5.71e-36 127
Msa1026330 AT1G65360.1 35.556 225 121 7 5 211 3 221 7.10e-34 122
Msa1026330 AT4G36590.1 39.560 182 102 3 5 179 3 183 1.56e-32 119
Msa1026330 AT3G04100.1 32.934 167 98 4 2 159 7 168 8.16e-24 95.1
Msa1026330 AT2G34440.1 38.938 113 68 1 9 121 2 113 1.86e-20 85.5
Msa1026330 AT3G66656.1 37.815 119 70 1 9 123 2 120 1.17e-19 83.6
Msa1026330 AT1G28460.1 32.778 180 107 4 5 177 4 176 1.46e-19 83.2
Msa1026330 AT1G72350.1 52.055 73 33 1 9 81 43 113 2.47e-18 80.9
Msa1026330 AT4G09960.2 38.000 100 52 1 9 108 2 91 2.06e-17 78.6
Msa1026330 AT4G09960.1 38.000 100 52 1 9 108 2 91 2.88e-17 78.2
Msa1026330 AT4G09960.6 38.000 100 52 1 9 108 3 92 3.15e-17 78.2
Msa1026330 AT4G09960.5 38.000 100 52 1 9 108 12 101 4.16e-17 78.2
Msa1026330 AT2G45660.2 33.835 133 63 2 12 119 5 137 4.40e-17 76.3
Msa1026330 AT4G09960.3 39.024 123 65 3 9 126 28 145 5.14e-17 78.2
Msa1026330 AT4G09960.4 39.024 123 65 3 9 126 97 214 1.23e-16 78.2
Msa1026330 AT2G45660.1 33.835 133 63 2 12 119 5 137 1.47e-16 75.9
Msa1026330 AT4G18960.1 38.318 107 56 1 2 108 11 107 1.62e-16 76.6
Msa1026330 AT2G22540.3 51.515 66 32 0 11 76 4 69 1.64e-16 76.3
Msa1026330 AT2G22540.1 51.515 66 32 0 11 76 4 69 1.64e-16 76.3
Msa1026330 AT2G45650.1 48.529 68 35 0 9 76 2 69 3.63e-16 75.5
Msa1026330 AT1G29962.1 30.435 184 109 6 5 177 4 179 3.98e-16 74.3
Msa1026330 AT4G22950.2 44.595 74 41 0 12 85 5 78 4.47e-16 73.9
Msa1026330 AT5G51870.2 52.308 65 31 0 12 76 5 69 1.18e-15 72.8
Msa1026330 AT5G51870.1 52.308 65 31 0 12 76 5 69 1.73e-15 73.2
Msa1026330 AT4G22950.3 44.000 75 42 0 12 86 5 79 2.46e-15 72.8
Msa1026330 AT4G22950.1 44.000 75 42 0 12 86 5 79 2.46e-15 72.8
Msa1026330 AT5G51870.4 52.308 65 31 0 12 76 5 69 2.70e-15 72.8
Msa1026330 AT5G51870.3 52.308 65 31 0 12 76 15 79 2.97e-15 72.8
Msa1026330 AT5G51870.5 52.308 65 31 0 12 76 28 92 3.09e-15 72.0
Msa1026330 AT1G22130.1 39.785 93 55 1 9 100 2 94 3.73e-15 73.9
Msa1026330 AT1G28450.1 31.285 179 103 6 5 168 4 177 4.06e-15 71.6
Msa1026330 AT1G77980.1 46.479 71 37 1 9 78 2 72 7.73e-15 72.8
Msa1026330 AT4G24540.1 41.667 96 48 2 11 98 4 99 8.19e-15 71.2
Msa1026330 AT3G57230.2 42.647 68 39 0 9 76 2 69 1.15e-14 69.3
Msa1026330 AT1G77950.3 43.421 76 41 2 9 83 2 76 1.40e-14 71.2
Msa1026330 AT1G77950.2 43.421 76 41 2 9 83 2 76 1.40e-14 71.2
Msa1026330 AT1G77950.1 43.421 76 41 2 9 83 2 76 1.40e-14 71.2
Msa1026330 AT1G77950.4 43.421 76 41 2 9 83 2 76 1.40e-14 71.2
Msa1026330 AT5G62165.14 46.154 65 35 0 12 76 5 69 1.59e-14 67.8
Msa1026330 AT5G62165.17 46.154 65 35 0 12 76 5 69 2.28e-14 68.9
Msa1026330 AT5G62165.12 46.154 65 35 0 12 76 5 69 2.28e-14 68.9
Msa1026330 AT5G62165.11 46.154 65 35 0 12 76 5 69 2.28e-14 68.9
Msa1026330 AT5G13790.2 48.529 68 35 0 9 76 2 69 2.33e-14 70.9
Msa1026330 AT3G54340.1 35.537 121 64 4 12 125 5 118 2.41e-14 70.5
Msa1026330 AT5G13790.1 48.529 68 35 0 9 76 2 69 2.47e-14 70.9
Msa1026330 AT2G22540.2 50.000 66 28 1 11 76 4 64 3.36e-14 70.1
Msa1026330 AT5G62165.6 46.154 65 35 0 12 76 5 69 3.58e-14 68.2
Msa1026330 AT5G62165.9 46.154 65 35 0 12 76 5 69 3.58e-14 68.2
Msa1026330 AT5G62165.7 46.154 65 35 0 12 76 5 69 3.58e-14 68.2
Msa1026330 AT5G62165.8 46.154 65 35 0 12 76 5 69 3.58e-14 68.2
Msa1026330 AT5G62165.13 46.154 65 35 0 12 76 5 69 3.58e-14 68.2
Msa1026330 AT4G37940.3 44.118 68 38 0 9 76 2 69 3.76e-14 68.6
Msa1026330 AT5G10140.2 46.377 69 36 1 9 76 2 70 4.59e-14 68.2
Msa1026330 AT5G51860.2 47.692 65 34 0 12 76 5 69 4.60e-14 68.9
Msa1026330 AT2G42830.1 44.118 68 38 0 9 76 17 84 4.75e-14 69.7
Msa1026330 AT4G37940.4 44.118 68 38 0 9 76 2 69 5.00e-14 69.3
Msa1026330 AT2G42830.2 44.118 68 38 0 9 76 17 84 5.28e-14 69.7
Msa1026330 AT3G58780.5 43.662 71 40 0 6 76 20 90 5.30e-14 69.7
Msa1026330 AT5G51860.1 47.692 65 34 0 12 76 5 69 5.60e-14 68.9
Msa1026330 AT4G37940.1 44.118 68 38 0 9 76 2 69 5.68e-14 69.3
Msa1026330 AT3G58780.1 43.662 71 40 0 6 76 14 84 5.72e-14 69.7
Msa1026330 AT1G47760.1 37.624 101 49 2 9 109 2 88 6.30e-14 68.2
Msa1026330 AT3G57390.3 45.588 68 37 0 9 76 2 69 7.20e-14 68.9
Msa1026330 AT5G10140.3 46.377 69 36 1 9 76 2 70 7.38e-14 68.2
Msa1026330 AT5G10140.4 46.377 69 36 1 9 76 2 70 7.67e-14 68.2
Msa1026330 AT5G10140.1 46.377 69 36 1 9 76 2 70 9.35e-14 68.2
Msa1026330 AT5G62165.10 46.154 65 35 0 12 76 5 69 9.94e-14 67.8
Msa1026330 AT5G62165.4 46.154 65 35 0 12 76 5 69 9.94e-14 67.8
Msa1026330 AT5G62165.3 46.154 65 35 0 12 76 5 69 1.00e-13 68.2
Msa1026330 AT5G62165.1 46.154 65 35 0 12 76 5 69 1.00e-13 68.2
Msa1026330 AT5G62165.2 46.154 65 35 0 12 76 5 69 1.00e-13 68.2
Msa1026330 AT3G58780.4 43.662 71 40 0 6 76 14 84 1.01e-13 69.3
Msa1026330 AT3G57230.1 42.647 68 39 0 9 76 2 69 1.09e-13 68.6
Msa1026330 AT3G57230.4 42.647 68 39 0 9 76 2 69 1.09e-13 68.6
Msa1026330 AT1G17310.1 33.929 168 70 4 8 170 47 178 1.23e-13 68.2
Msa1026330 AT3G57390.1 45.588 68 37 0 9 76 2 69 1.42e-13 68.6
Msa1026330 AT1G17310.2 33.929 168 70 4 8 170 58 189 1.50e-13 68.2
Msa1026330 AT2G22630.1 44.776 67 37 0 9 75 2 68 1.89e-13 67.8
Msa1026330 AT2G22630.2 44.776 67 37 0 9 75 2 68 1.89e-13 67.8
Msa1026330 AT3G61120.1 45.588 68 37 0 9 76 2 69 1.93e-13 68.2
Msa1026330 AT3G30260.1 42.029 69 39 1 9 76 2 70 3.05e-13 67.4
Msa1026330 AT4G11880.2 45.714 70 37 1 12 80 5 74 3.24e-13 66.2
Msa1026330 AT4G11880.3 45.714 70 37 1 12 80 5 74 3.24e-13 66.2
Msa1026330 AT5G60910.1 46.377 69 34 1 9 77 2 67 3.97e-13 67.0
Msa1026330 AT1G26310.1 43.478 69 38 1 9 76 2 70 5.25e-13 67.0
Msa1026330 AT4G11880.4 45.714 70 37 1 12 80 5 74 6.44e-13 66.2
Msa1026330 AT4G11880.1 45.714 70 37 1 12 80 5 74 6.44e-13 66.2
Msa1026330 AT5G15800.1 44.928 69 37 1 9 76 2 70 8.91e-13 66.2
Msa1026330 AT5G23260.1 45.333 75 39 1 5 77 14 88 9.25e-13 66.2
Msa1026330 AT5G23260.4 45.333 75 39 1 5 77 14 88 9.34e-13 66.2
Msa1026330 AT1G69120.2 40.580 69 40 1 9 76 2 70 1.06e-12 65.9
Msa1026330 AT5G15800.2 44.928 69 37 1 9 76 2 70 1.28e-12 65.9
Msa1026330 AT2G14210.1 42.029 69 39 1 9 76 2 70 1.66e-12 65.1
Msa1026330 AT1G69120.1 40.580 69 40 1 9 76 2 70 1.89e-12 65.5
Msa1026330 AT5G23260.3 47.887 71 35 1 9 77 2 72 2.04e-12 65.1
Msa1026330 AT5G23260.2 47.887 71 35 1 9 77 2 72 2.08e-12 65.1
Msa1026330 AT2G14210.2 42.029 69 39 1 9 76 2 70 2.18e-12 65.1
Msa1026330 AT5G20240.2 46.774 62 33 0 9 70 2 63 2.64e-12 63.2
Msa1026330 AT5G65050.2 43.478 69 38 1 9 76 2 70 2.93e-12 63.5
Msa1026330 AT5G65050.5 43.478 69 38 1 9 76 2 70 3.51e-12 63.5
Msa1026330 AT2G03710.3 48.214 56 29 0 9 64 2 57 4.02e-12 63.5
Msa1026330 AT5G65050.3 43.478 69 38 1 9 76 2 70 4.11e-12 63.5
Msa1026330 AT5G65050.1 43.478 69 38 1 9 76 2 70 4.38e-12 63.2
Msa1026330 AT3G02310.2 43.478 69 38 1 9 76 2 70 4.47e-12 63.5
Msa1026330 AT5G20240.1 44.000 75 38 2 9 79 2 76 4.83e-12 63.5
Msa1026330 AT3G58780.2 45.902 61 33 0 6 66 14 74 5.00e-12 63.9
Msa1026330 AT1G24260.3 50.000 56 28 0 9 64 2 57 5.27e-12 63.9
Msa1026330 AT1G24260.1 50.000 56 28 0 9 64 2 57 5.73e-12 63.9
Msa1026330 AT1G24260.2 50.000 56 28 0 9 64 2 57 5.79e-12 63.9
Msa1026330 AT3G58780.3 45.902 61 33 0 6 66 14 74 6.77e-12 63.9
Msa1026330 AT2G03710.1 48.214 56 29 0 9 64 2 57 9.74e-12 63.5
Msa1026330 AT2G03710.2 48.214 56 29 0 9 64 2 57 9.94e-12 63.5
Msa1026330 AT3G02310.1 50.000 56 28 0 9 64 2 57 1.02e-11 63.2
Msa1026330 AT5G65060.2 42.029 69 39 1 9 76 2 70 1.38e-11 62.0
Msa1026330 AT5G65060.3 42.029 69 39 1 9 76 2 70 1.69e-11 61.6
Msa1026330 AT5G65060.1 42.029 69 39 1 9 76 2 70 1.79e-11 61.6
Msa1026330 AT5G65070.4 43.333 60 34 0 9 68 2 61 2.58e-11 60.5
Msa1026330 AT1G18750.4 26.667 195 123 9 9 187 2 192 3.58e-11 62.4
Msa1026330 AT1G18750.1 26.667 195 123 9 9 187 2 192 4.04e-11 62.4
Msa1026330 AT5G65070.1 30.612 147 95 4 9 152 2 144 4.71e-11 60.8
Msa1026330 AT1G71692.1 38.806 67 40 1 12 77 5 71 4.77e-11 60.8
Msa1026330 AT1G18750.3 34.831 89 53 3 9 93 2 89 6.38e-11 62.0
Msa1026330 AT5G65070.5 45.614 57 31 0 9 65 2 58 9.68e-11 60.1

Find 52 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTATTCAAGAAGATTT+TGG 0.235888 7_1:-58088409 Msa1026330:CDS
ATCTCAGTTTGTTGAGGATT+TGG 0.255732 7_1:+58088264 None:intergenic
ATGTTGCAACCTCATTTAAT+TGG 0.256996 7_1:-58088213 Msa1026330:CDS
TGTTGATAGGAGTGCAATTC+AGG 0.259070 7_1:-58088358 Msa1026330:CDS
TTTAATTGGCTTCAACAATA+TGG 0.267629 7_1:-58088199 Msa1026330:CDS
AAATCATTACCAAGAGAAAA+TGG 0.314064 7_1:+58088315 None:intergenic
AATATGGAAGGAGGAGGATA+TGG 0.330377 7_1:-58088183 Msa1026330:CDS
AGGCGTTCAAGGCTCAATTT+TGG 0.334747 7_1:-58088470 Msa1026330:CDS
GCCCTATTCATTCTATCAAT+TGG 0.337270 7_1:+58088438 None:intergenic
GTCATAGATCGCCATCTCTC+TGG 0.351921 7_1:-58088639 Msa1026330:CDS
CCTTGAACGCCTTGCACAAA+AGG 0.373825 7_1:+58088481 None:intergenic
GTTGAGGATTTGGCTGATAA+TGG 0.382243 7_1:+58088274 None:intergenic
TCCAATTGATAGAATGAATA+GGG 0.395255 7_1:-58088439 Msa1026330:CDS
AATGAGCTTTGCACCCTTTG+TGG 0.404337 7_1:-58088735 Msa1026330:CDS
TTGTTGCTCAACATGTTGAT+AGG 0.407276 7_1:-58088371 Msa1026330:CDS
CTAGAAACTTCAATAGAATC+CGG 0.407513 7_1:+58088155 None:intergenic
TCTATGACTGTATCAACATT+TGG 0.411655 7_1:+58088654 None:intergenic
ATTAAATGAGGTTGCAACAT+AGG 0.428043 7_1:+58088216 None:intergenic
GTCCAATTGATAGAATGAAT+AGG 0.444780 7_1:-58088440 Msa1026330:CDS
ATAATGGAGAGGGATGTTAG+AGG 0.448969 7_1:+58088290 None:intergenic
ATCTTTCCTTCCGCTCGACA+TGG 0.482389 7_1:+58088854 None:intergenic
TTAAATGAGGTTGCAACATA+GGG 0.493554 7_1:+58088217 None:intergenic
AGCATTGAGCTCACGCACTT+TGG 0.503646 7_1:+58088563 None:intergenic
AGCAACATCTACACCACAAA+GGG 0.507315 7_1:+58088722 None:intergenic
TCATAGATCGCCATCTCTCT+GGG 0.511452 7_1:-58088638 Msa1026330:CDS
TAGAAACTTCAATAGAATCC+GGG 0.517746 7_1:+58088156 None:intergenic
TGTATTCTCACCTAGTGAAA+AGG 0.518205 7_1:-58088694 Msa1026330:CDS
GTTGATAGGAGTGCAATTCA+GGG 0.537321 7_1:-58088357 Msa1026330:CDS
ATTGGCTTCAACAATATGGA+AGG 0.538957 7_1:-58088195 Msa1026330:CDS
GCTTTGAGAAAGTCACTTGG+AGG 0.544594 7_1:+58088784 None:intergenic
TTGTTGAAGCCAATTAAATG+AGG 0.551491 7_1:+58088204 None:intergenic
GAAAACATCTCAGTTTGTTG+AGG 0.554882 7_1:+58088258 None:intergenic
GCGGTGGGATCCCAGAGAGA+TGG 0.573036 7_1:+58088628 None:intergenic
CTTCAACCATGTCGAGCGGA+AGG 0.578339 7_1:-58088860 None:intergenic
CTTTCTCAAAGCGTCGTAGT+GGG 0.584950 7_1:-58088773 Msa1026330:CDS
AATCAACAACACACTGGACG+AGG 0.587728 7_1:-58088529 Msa1026330:CDS
ATGAGCCTCAATGAATTCCA+TGG 0.597814 7_1:+58088593 None:intergenic
TTCAACAATATGGAAGGAGG+AGG 0.598809 7_1:-58088189 Msa1026330:CDS
ACTTTCTCAAAGCGTCGTAG+TGG 0.602122 7_1:-58088774 Msa1026330:CDS
GACGCTTTGAGAAAGTCACT+TGG 0.608413 7_1:+58088781 None:intergenic
AGAAACTTCAATAGAATCCG+GGG 0.619380 7_1:+58088157 None:intergenic
GGATTTGGCTGATAATGGAG+AGG 0.633381 7_1:+58088279 None:intergenic
GGCTTCAACAATATGGAAGG+AGG 0.642874 7_1:-58088192 Msa1026330:CDS
GTTTCAAATCAACAACACAC+TGG 0.650841 7_1:-58088535 Msa1026330:CDS
CAGCACCATGGAATTCATTG+AGG 0.664382 7_1:-58088598 Msa1026330:CDS
ACCGCAAAACAACAGCACCA+TGG 0.667033 7_1:-58088610 Msa1026330:CDS
GAAACTTCAATAGAATCCGG+GGG 0.679421 7_1:+58088158 None:intergenic
GATTTGGCTGATAATGGAGA+GGG 0.685158 7_1:+58088280 None:intergenic
GAGCAACATCTACACCACAA+AGG 0.689768 7_1:+58088721 None:intergenic
TGTACTTCAACCATGTCGAG+CGG 0.700229 7_1:-58088864 None:intergenic
TCGAGCGGAAGGAAAGATCG+TGG 0.717660 7_1:-58088849 Msa1026330:CDS
AAACTTCAATAGAATCCGGG+GGG 0.771286 7_1:+58088159 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTATTCAAGAAGATTT+TGG - chr7_1:58088607-58088626 Msa1026330:CDS 20.0%
!! AAAGAAAATACCTTTTCACT+AGG + chr7_1:58088335-58088354 None:intergenic 25.0%
!!! AGTGAAAAGGTATTTTCTTT+CGG - chr7_1:58088335-58088354 Msa1026330:CDS 25.0%
! TCCAATTGATAGAATGAATA+GGG - chr7_1:58088577-58088596 Msa1026330:CDS 25.0%
!! ATTATTCAAGAAGATTTTGG+TGG - chr7_1:58088610-58088629 Msa1026330:CDS 25.0%
! AAATCATTACCAAGAGAAAA+TGG + chr7_1:58088704-58088723 None:intergenic 25.0%
! ATAGGGTTATGAAAAATTTG+TGG + chr7_1:58088785-58088804 None:intergenic 25.0%
!! TTTAATTGGCTTCAACAATA+TGG - chr7_1:58088817-58088836 Msa1026330:CDS 25.0%
!! TCTTTTTCATCTCGATCTTT+TGG + chr7_1:58088196-58088215 None:intergenic 30.0%
TCTATGACTGTATCAACATT+TGG + chr7_1:58088365-58088384 None:intergenic 30.0%
GTCCAATTGATAGAATGAAT+AGG - chr7_1:58088576-58088595 Msa1026330:CDS 30.0%
TTAAATGAGGTTGCAACATA+GGG + chr7_1:58088802-58088821 None:intergenic 30.0%
ATTAAATGAGGTTGCAACAT+AGG + chr7_1:58088803-58088822 None:intergenic 30.0%
ATGTTGCAACCTCATTTAAT+TGG - chr7_1:58088803-58088822 Msa1026330:CDS 30.0%
TTGTTGAAGCCAATTAAATG+AGG + chr7_1:58088815-58088834 None:intergenic 30.0%
TGTATTCTCACCTAGTGAAA+AGG - chr7_1:58088322-58088341 Msa1026330:CDS 35.0%
GTTTCAAATCAACAACACAC+TGG - chr7_1:58088481-58088500 Msa1026330:CDS 35.0%
GCCCTATTCATTCTATCAAT+TGG + chr7_1:58088581-58088600 None:intergenic 35.0%
TTGTTGCTCAACATGTTGAT+AGG - chr7_1:58088645-58088664 Msa1026330:CDS 35.0%
! CACACTTTTCCATTTTCTCT+TGG - chr7_1:58088692-58088711 Msa1026330:CDS 35.0%
!! ATCTCAGTTTGTTGAGGATT+TGG + chr7_1:58088755-58088774 None:intergenic 35.0%
!! GAAAACATCTCAGTTTGTTG+AGG + chr7_1:58088761-58088780 None:intergenic 35.0%
! ATTGGCTTCAACAATATGGA+AGG - chr7_1:58088821-58088840 Msa1026330:CDS 35.0%
AGCAACATCTACACCACAAA+GGG + chr7_1:58088297-58088316 None:intergenic 40.0%
ATGAGCCTCAATGAATTCCA+TGG + chr7_1:58088426-58088445 None:intergenic 40.0%
!! GTTCAAGGCTCAATTTTGGT+GGG - chr7_1:58088550-58088569 Msa1026330:CDS 40.0%
! TGTTGATAGGAGTGCAATTC+AGG - chr7_1:58088658-58088677 Msa1026330:CDS 40.0%
! GTTGATAGGAGTGCAATTCA+GGG - chr7_1:58088659-58088678 Msa1026330:CDS 40.0%
! ATAATGGAGAGGGATGTTAG+AGG + chr7_1:58088729-58088748 None:intergenic 40.0%
!! GATTTGGCTGATAATGGAGA+GGG + chr7_1:58088739-58088758 None:intergenic 40.0%
GTTGAGGATTTGGCTGATAA+TGG + chr7_1:58088745-58088764 None:intergenic 40.0%
TTCAACAATATGGAAGGAGG+AGG - chr7_1:58088827-58088846 Msa1026330:CDS 40.0%
AATATGGAAGGAGGAGGATA+TGG - chr7_1:58088833-58088852 Msa1026330:CDS 40.0%
!! GCTTTGAGAAAGTCACTTGG+AGG + chr7_1:58088235-58088254 None:intergenic 45.0%
!! GACGCTTTGAGAAAGTCACT+TGG + chr7_1:58088238-58088257 None:intergenic 45.0%
!! ACTTTCTCAAAGCGTCGTAG+TGG - chr7_1:58088242-58088261 Msa1026330:CDS 45.0%
!! CTTTCTCAAAGCGTCGTAGT+GGG - chr7_1:58088243-58088262 Msa1026330:CDS 45.0%
AATGAGCTTTGCACCCTTTG+TGG - chr7_1:58088281-58088300 Msa1026330:CDS 45.0%
GAGCAACATCTACACCACAA+AGG + chr7_1:58088298-58088317 None:intergenic 45.0%
TCATAGATCGCCATCTCTCT+GGG - chr7_1:58088378-58088397 Msa1026330:CDS 45.0%
!!! TCCATGGTGCTGTTGTTTTG+CGG + chr7_1:58088410-58088429 None:intergenic 45.0%
CAGCACCATGGAATTCATTG+AGG - chr7_1:58088418-58088437 Msa1026330:CDS 45.0%
AATCAACAACACACTGGACG+AGG - chr7_1:58088487-58088506 Msa1026330:CDS 45.0%
!! TGAGTTGAGCCTTTTGTGCA+AGG - chr7_1:58088526-58088545 Msa1026330:CDS 45.0%
! AGGCGTTCAAGGCTCAATTT+TGG - chr7_1:58088546-58088565 Msa1026330:CDS 45.0%
! CGTTCAAGGCTCAATTTTGG+TGG - chr7_1:58088549-58088568 Msa1026330:CDS 45.0%
!! GGATTTGGCTGATAATGGAG+AGG + chr7_1:58088740-58088759 None:intergenic 45.0%
GGCTTCAACAATATGGAAGG+AGG - chr7_1:58088824-58088843 Msa1026330:CDS 45.0%
GTCATAGATCGCCATCTCTC+TGG - chr7_1:58088377-58088396 Msa1026330:CDS 50.0%
!!! TGGTGCTGTTGTTTTGCGGT+GGG + chr7_1:58088406-58088425 None:intergenic 50.0%
!!! ATGGTGCTGTTGTTTTGCGG+TGG + chr7_1:58088407-58088426 None:intergenic 50.0%
ACCGCAAAACAACAGCACCA+TGG - chr7_1:58088406-58088425 Msa1026330:CDS 50.0%
! AGCATTGAGCTCACGCACTT+TGG + chr7_1:58088456-58088475 None:intergenic 50.0%
CTGGACGAGGAGAAAAAGTG+TGG - chr7_1:58088500-58088519 Msa1026330:CDS 50.0%
CCTTGAACGCCTTGCACAAA+AGG + chr7_1:58088538-58088557 None:intergenic 50.0%
!! CCTTTTGTGCAAGGCGTTCA+AGG - chr7_1:58088535-58088554 Msa1026330:CDS 50.0%
TCGAGCGGAAGGAAAGATCG+TGG - chr7_1:58088167-58088186 Msa1026330:CDS 55.0%
GCGGTGGGATCCCAGAGAGA+TGG + chr7_1:58088391-58088410 None:intergenic 65.0%
GGAGGAGGATATGGACCCCC+CGG - chr7_1:58088842-58088861 Msa1026330:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 58088164 58088874 58088164 ID=Msa1026330;Name=Msa1026330
chr7_1 mRNA 58088164 58088874 58088164 ID=Msa1026330-mRNA-1;Parent=Msa1026330;Name=Msa1026330-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|236
chr7_1 exon 58088164 58088874 58088164 ID=Msa1026330-mRNA-1:exon:11616;Parent=Msa1026330-mRNA-1
chr7_1 CDS 58088164 58088874 58088164 ID=Msa1026330-mRNA-1:cds;Parent=Msa1026330-mRNA-1
Gene Sequence

>Msa1026330

ATGTCGAGCGGAAGGAAAGATCGTGGTAGCCAAAAGATCGAGATGAAAAAGATGAGCAACGAGAGCAACCTCCAAGTGACTTTCTCAAAGCGTCGTAGTGGGCTCTTCAAGAAAGTCAATGAGCTTTGCACCCTTTGTGGTGTAGATGTTGCTCTCATTGTATTCTCACCTAGTGAAAAGGTATTTTCTTTCGGTCATCCAAATGTTGATACAGTCATAGATCGCCATCTCTCTGGGATCCCACCGCAAAACAACAGCACCATGGAATTCATTGAGGCTCATCGTAGCGCCAAAGTGCGTGAGCTCAATGCTGAGTTGTTTCAAATCAACAACACACTGGACGAGGAGAAAAAGTGTGGTGATGAGTTGAGCCTTTTGTGCAAGGCGTTCAAGGCTCAATTTTGGTGGGCTTGTCCAATTGATAGAATGAATAGGGCTCAGCTTGAATTATTCAAGAAGATTTTGGTGGAGCTTAAGAAACTTGTTGCTCAACATGTTGATAGGAGTGCAATTCAGGGTGCTTCTGCTCACACTTTTCCATTTTCTCTTGGTAATGATTTATCCTCTAACATCCCTCTCCATTATCAGCCAAATCCTCAACAAACTGAGATGTTTTCGCCACAAATTTTTCATAACCCTATGTTGCAACCTCATTTAATTGGCTTCAACAATATGGAAGGAGGAGGATATGGACCCCCCGGATTCTATTGA
Protein sequence

>Msa1026330

MSSGRKDRGSQKIEMKKMSNESNLQVTFSKRRSGLFKKVNELCTLCGVDVALIVFSPSEKVFSFGHPNVDTVIDRHLSGIPPQNNSTMEFIEAHRSAKVRELNAELFQINNTLDEEKKCGDELSLLCKAFKAQFWWACPIDRMNRAQLELFKKILVELKKLVAQHVDRSAIQGASAHTFPFSLGNDLSSNIPLHYQPNPQQTEMFSPQIFHNPMLQPHLIGFNNMEGGGYGPPGFY*