Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1036110 | KEH23820.1 | 80.851 | 47 | 8 | 1 | 32 | 77 | 10 | 56 | 2.82e-19 | 85.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1036110 | sp|Q8W3L9|EIL2_ORYSJ | 68.293 | 41 | 13 | 0 | 37 | 77 | 148 | 188 | 8.74e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1036110 | A0A072UDC0 | 80.851 | 47 | 8 | 1 | 32 | 77 | 10 | 56 | 1.35e-19 | 85.5 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1036110 | TF | EIL |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1036110 | MtrunA17_Chr7g0260281 | 80.851 | 47 | 8 | 1 | 32 | 77 | 10 | 56 | 7.49e-23 | 85.1 |
| Msa1036110 | MtrunA17_Chr2g0329151 | 72.727 | 33 | 9 | 0 | 45 | 77 | 106 | 138 | 1.64e-12 | 61.2 |
| Msa1036110 | MtrunA17_Chr5g0440591 | 75.758 | 33 | 8 | 0 | 45 | 77 | 115 | 147 | 2.86e-12 | 60.5 |
| Msa1036110 | MtrunA17_Chr3g0118361 | 75.758 | 33 | 8 | 0 | 45 | 77 | 118 | 150 | 3.59e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1036110 | AT3G20770.1 | 78.788 | 33 | 7 | 0 | 45 | 77 | 115 | 147 | 9.62e-13 | 62.4 |
| Msa1036110 | AT1G73730.1 | 69.697 | 33 | 10 | 0 | 45 | 77 | 106 | 138 | 9.09e-12 | 59.7 |
| Msa1036110 | AT1G73730.2 | 69.697 | 33 | 10 | 0 | 45 | 77 | 94 | 126 | 9.72e-12 | 59.3 |
| Msa1036110 | AT2G27050.1 | 75.758 | 33 | 8 | 0 | 45 | 77 | 116 | 148 | 1.05e-11 | 59.3 |
Find 14 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCAATGAAGAAAGAAATT+TGG | 0.267238 | 7_1:+72293530 | Msa1036110:CDS |
| TACGGAATTATACCTGAAAA+TGG | 0.269291 | 7_1:+72293715 | Msa1036110:CDS |
| GAATATAAACGGTGGTATCA+TGG | 0.436629 | 7_1:+72293555 | Msa1036110:CDS |
| CTGAAAATCTTCACTGTTGG+TGG | 0.437559 | 7_1:+72293758 | Msa1036110:CDS |
| GAAATTTGGAGGAATATAAA+CGG | 0.490162 | 7_1:+72293544 | Msa1036110:CDS |
| ATTGTAGAACAAGCACAAGT+TGG | 0.508697 | 7_1:+72293666 | Msa1036110:intron |
| ATGATCATGTTGTATCAGAA+AGG | 0.538951 | 7_1:+72293806 | Msa1036110:CDS |
| CAATGAAGAAAGAAATTTGG+AGG | 0.552046 | 7_1:+72293533 | Msa1036110:CDS |
| CATCTGAAAATCTTCACTGT+TGG | 0.553597 | 7_1:+72293755 | Msa1036110:CDS |
| AACAAGCACAAGTTGGAAGA+TGG | 0.555633 | 7_1:+72293673 | Msa1036110:CDS |
| AAATCTTCACTGTTGGTGGA+AGG | 0.565627 | 7_1:+72293762 | Msa1036110:CDS |
| CATTCTGATGAATTTGTGTA+CGG | 0.572592 | 7_1:+72293697 | Msa1036110:CDS |
| ATTTGGAGGAATATAAACGG+TGG | 0.680669 | 7_1:+72293547 | Msa1036110:CDS |
| AGTACACAAGTTGAAGACAA+AGG | 0.686224 | 7_1:+72293510 | Msa1036110:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGAACAAGATAAAAA+AGG | + | chr7_1:72293616-72293635 | Msa1036110:intron | 15.0% |
| ! | GAAATTTGGAGGAATATAAA+CGG | + | chr7_1:72293544-72293563 | Msa1036110:CDS | 25.0% |
| ! | GGAATTGAAAAATCATAGTA+AGG | + | chr7_1:72293576-72293595 | Msa1036110:CDS | 25.0% |
| !! | TTATCTTGTTCAATTTTTGC+TGG | - | chr7_1:72293613-72293632 | None:intergenic | 25.0% |
| AGGCAATGAAGAAAGAAATT+TGG | + | chr7_1:72293530-72293549 | Msa1036110:CDS | 30.0% | |
| CAATGAAGAAAGAAATTTGG+AGG | + | chr7_1:72293533-72293552 | Msa1036110:CDS | 30.0% | |
| CATTCTGATGAATTTGTGTA+CGG | + | chr7_1:72293697-72293716 | Msa1036110:CDS | 30.0% | |
| TACGGAATTATACCTGAAAA+TGG | + | chr7_1:72293715-72293734 | Msa1036110:CDS | 30.0% | |
| !!! | CTTATTGGTTTTCCATTTTC+AGG | - | chr7_1:72293730-72293749 | None:intergenic | 30.0% |
| ! | TTTTCAGATGCAACACTTAT+TGG | - | chr7_1:72293745-72293764 | None:intergenic | 30.0% |
| ATGATCATGTTGTATCAGAA+AGG | + | chr7_1:72293806-72293825 | Msa1036110:CDS | 30.0% | |
| ! | AGTACACAAGTTGAAGACAA+AGG | + | chr7_1:72293510-72293529 | Msa1036110:CDS | 35.0% |
| ATTTGGAGGAATATAAACGG+TGG | + | chr7_1:72293547-72293566 | Msa1036110:CDS | 35.0% | |
| GAATATAAACGGTGGTATCA+TGG | + | chr7_1:72293555-72293574 | Msa1036110:CDS | 35.0% | |
| ATTGTAGAACAAGCACAAGT+TGG | + | chr7_1:72293666-72293685 | Msa1036110:intron | 35.0% | |
| CATCTGAAAATCTTCACTGT+TGG | + | chr7_1:72293755-72293774 | Msa1036110:CDS | 35.0% | |
| ! | AACAAGCACAAGTTGGAAGA+TGG | + | chr7_1:72293673-72293692 | Msa1036110:CDS | 40.0% |
| CTGAAAATCTTCACTGTTGG+TGG | + | chr7_1:72293758-72293777 | Msa1036110:CDS | 40.0% | |
| ! | AAATCTTCACTGTTGGTGGA+AGG | + | chr7_1:72293762-72293781 | Msa1036110:CDS | 40.0% |
| ! | GTTGGTGGAAGGAAAAAGTG+AGG | + | chr7_1:72293773-72293792 | Msa1036110:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 72293505 | 72293840 | 72293505 | ID=Msa1036110;Name=Msa1036110 |
| chr7_1 | mRNA | 72293505 | 72293840 | 72293505 | ID=Msa1036110-mRNA-1;Parent=Msa1036110;Name=Msa1036110-mRNA-1;_AED=0.31;_eAED=0.44;_QI=0|0|0|0.5|1|1|2|0|87 |
| chr7_1 | exon | 72293505 | 72293600 | 72293505 | ID=Msa1036110-mRNA-1:exon:17636;Parent=Msa1036110-mRNA-1 |
| chr7_1 | exon | 72293673 | 72293840 | 72293673 | ID=Msa1036110-mRNA-1:exon:17637;Parent=Msa1036110-mRNA-1 |
| chr7_1 | CDS | 72293505 | 72293600 | 72293505 | ID=Msa1036110-mRNA-1:cds;Parent=Msa1036110-mRNA-1 |
| chr7_1 | CDS | 72293673 | 72293840 | 72293673 | ID=Msa1036110-mRNA-1:cds;Parent=Msa1036110-mRNA-1 |
| Gene Sequence |
| Protein sequence |
