Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043230 | A0A396GUT9 | 81.522 | 92 | 8 | 2 | 55 | 146 | 12 | 94 | 6.16e-33 | 124 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1043230 | TR | SNF2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043230 | MtrunA17_Chr7g0214291 | 81.522 | 92 | 8 | 2 | 55 | 146 | 12 | 94 | 1.19e-36 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCCATCACCATTTAATTT+TGG | 0.165025 | 7_2:-1174249 | None:intergenic |
| TTAAACTCTCCAAAATTAAA+TGG | 0.244854 | 7_2:+1174240 | Msa1043230:CDS |
| ATGATACTCTAAGCCTTAAT+TGG | 0.261799 | 7_2:+1173825 | Msa1043230:CDS |
| AGTGAAATATCATGATTTAA+AGG | 0.299376 | 7_2:+1174130 | Msa1043230:CDS |
| CAACAACTCAGGAGAATTAA+TGG | 0.337925 | 7_2:+1174026 | Msa1043230:CDS |
| AAATACCAATCAGCCAATTA+AGG | 0.350978 | 7_2:-1173838 | None:intergenic |
| CTAAGCCTTAATTGGCTGAT+TGG | 0.351787 | 7_2:+1173833 | Msa1043230:CDS |
| GAAGGATTCCTTTCCATCCT+AGG | 0.377685 | 7_2:-1173520 | None:intergenic |
| AAATACCTTCGATTGATAGA+AGG | 0.404032 | 7_2:-1173538 | None:intergenic |
| TATTTCACTGTATTTAGCTC+TGG | 0.423345 | 7_2:-1174116 | None:intergenic |
| CCAATGGTCCATCATCAACT+TGG | 0.427516 | 7_2:-1174069 | None:intergenic |
| TTTAAAGGAAGCTGATTTGA+AGG | 0.442829 | 7_2:+1174145 | Msa1043230:CDS |
| AATAGGCAGTAACGTGGCGC+TGG | 0.447524 | 7_2:+1173796 | Msa1043230:intron |
| TTGTTATTGTCGTTGCTCCT+AGG | 0.483705 | 7_2:+1173503 | Msa1043230:CDS |
| TGTTGCAAACGATGTTGAAA+AGG | 0.485389 | 7_2:+1174214 | Msa1043230:CDS |
| TCTCCAAAATTAAATGGTGA+TGG | 0.515919 | 7_2:+1174246 | Msa1043230:CDS |
| TATTGTCGTTGCTCCTAGGA+TGG | 0.520917 | 7_2:+1173507 | Msa1043230:CDS |
| GCTGATGATGATGGTGGGAG+TGG | 0.523385 | 7_2:+1173475 | Msa1043230:CDS |
| TCTCACGCCCAAGTTGATGA+TGG | 0.528994 | 7_2:+1174061 | Msa1043230:CDS |
| CCAAGTTGATGATGGACCAT+TGG | 0.554041 | 7_2:+1174069 | Msa1043230:CDS |
| TATTAATCTTATCAATCCAA+TGG | 0.563475 | 7_2:-1174085 | None:intergenic |
| ATAGGCAGTAACGTGGCGCT+GGG | 0.577207 | 7_2:+1173797 | Msa1043230:intron |
| CAATGGTCCATCATCAACTT+GGG | 0.577723 | 7_2:-1174068 | None:intergenic |
| AAAGTTGCTGCTGATGATGA+TGG | 0.585831 | 7_2:+1173466 | Msa1043230:CDS |
| TATCATACTTGCAACAACTC+AGG | 0.593179 | 7_2:+1174015 | Msa1043230:CDS |
| TCGTTGCTCCTAGGATGGAA+AGG | 0.616152 | 7_2:+1173512 | Msa1043230:CDS |
| GGAATCCTTCTATCAATCGA+AGG | 0.618500 | 7_2:+1173533 | Msa1043230:CDS |
| TTGTTCAATAGGCAGTAACG+TGG | 0.663232 | 7_2:+1173790 | Msa1043230:intron |
| TTGCTGCTGATGATGATGGT+GGG | 0.685686 | 7_2:+1173470 | Msa1043230:CDS |
| TTGGGCGTGAGAATCAAATG+AGG | 0.687571 | 7_2:-1174050 | None:intergenic |
| GTTGCTGCTGATGATGATGG+TGG | 0.691465 | 7_2:+1173469 | Msa1043230:CDS |
| ATGGTTGTTGATATTGAACG+TGG | 0.759107 | 7_2:+1173442 | Msa1043230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAAGTTTTCTTTTATTTC+AGG | + | chr7_2:1173626-1173645 | Msa1043230:intron | 15.0% |
| !!! | AAGTTTTTTTAATTTTGTTC+AGG | + | chr7_2:1173706-1173725 | Msa1043230:intron | 15.0% |
| !!! | TTTAAGTTTTCTTTTATTTC+AGG | + | chr7_2:1173626-1173645 | Msa1043230:intron | 15.0% |
| !!! | AAGTTTTTTTAATTTTGTTC+AGG | + | chr7_2:1173706-1173725 | Msa1043230:intron | 15.0% |
| !! | AGTTTCTTTAATCCATTAAA+TGG | + | chr7_2:1173734-1173753 | Msa1043230:intron | 20.0% |
| !!! | AAGGTATTAACTATTGTTAT+GGG | + | chr7_2:1173896-1173915 | Msa1043230:intron | 20.0% |
| !! | TATTAATCTTATCAATCCAA+TGG | - | chr7_2:1174088-1174107 | None:intergenic | 20.0% |
| !! | AGTGAAATATCATGATTTAA+AGG | + | chr7_2:1174130-1174149 | Msa1043230:CDS | 20.0% |
| !! | TTAAACTCTCCAAAATTAAA+TGG | + | chr7_2:1174240-1174259 | Msa1043230:CDS | 20.0% |
| !! | AGTTTCTTTAATCCATTAAA+TGG | + | chr7_2:1173734-1173753 | Msa1043230:intron | 20.0% |
| !!! | AAGGTATTAACTATTGTTAT+GGG | + | chr7_2:1173896-1173915 | Msa1043230:intron | 20.0% |
| !! | TATTAATCTTATCAATCCAA+TGG | - | chr7_2:1174088-1174107 | None:intergenic | 20.0% |
| !! | AGTGAAATATCATGATTTAA+AGG | + | chr7_2:1174130-1174149 | Msa1043230:CDS | 20.0% |
| !! | TTAAACTCTCCAAAATTAAA+TGG | + | chr7_2:1174240-1174259 | Msa1043230:CDS | 20.0% |
| ! | AGATTAATGCAACCATTTAA+TGG | - | chr7_2:1173749-1173768 | None:intergenic | 25.0% |
| !!! | GAAGGTATTAACTATTGTTA+TGG | + | chr7_2:1173895-1173914 | Msa1043230:intron | 25.0% |
| ! | AGATTAATGCAACCATTTAA+TGG | - | chr7_2:1173749-1173768 | None:intergenic | 25.0% |
| !!! | GAAGGTATTAACTATTGTTA+TGG | + | chr7_2:1173895-1173914 | Msa1043230:intron | 25.0% |
| AAATACCTTCGATTGATAGA+AGG | - | chr7_2:1173541-1173560 | None:intergenic | 30.0% | |
| TTGCCCATTATTTGTTCAAT+AGG | + | chr7_2:1173779-1173798 | Msa1043230:intron | 30.0% | |
| CTGCCTATTGAACAAATAAT+GGG | - | chr7_2:1173785-1173804 | None:intergenic | 30.0% | |
| ACTGCCTATTGAACAAATAA+TGG | - | chr7_2:1173786-1173805 | None:intergenic | 30.0% | |
| ATGATACTCTAAGCCTTAAT+TGG | + | chr7_2:1173825-1173844 | Msa1043230:CDS | 30.0% | |
| AAATACCAATCAGCCAATTA+AGG | - | chr7_2:1173841-1173860 | None:intergenic | 30.0% | |
| ! | ATTTTCGATCGATCTATGAA+TGG | + | chr7_2:1173857-1173876 | Msa1043230:intron | 30.0% |
| ! | TATTTCACTGTATTTAGCTC+TGG | - | chr7_2:1174119-1174138 | None:intergenic | 30.0% |
| !! | TTTAAAGGAAGCTGATTTGA+AGG | + | chr7_2:1174145-1174164 | Msa1043230:CDS | 30.0% |
| AAATACCTTCGATTGATAGA+AGG | - | chr7_2:1173541-1173560 | None:intergenic | 30.0% | |
| TTGCCCATTATTTGTTCAAT+AGG | + | chr7_2:1173779-1173798 | Msa1043230:intron | 30.0% | |
| CTGCCTATTGAACAAATAAT+GGG | - | chr7_2:1173785-1173804 | None:intergenic | 30.0% | |
| ACTGCCTATTGAACAAATAA+TGG | - | chr7_2:1173786-1173805 | None:intergenic | 30.0% | |
| ATGATACTCTAAGCCTTAAT+TGG | + | chr7_2:1173825-1173844 | Msa1043230:CDS | 30.0% | |
| AAATACCAATCAGCCAATTA+AGG | - | chr7_2:1173841-1173860 | None:intergenic | 30.0% | |
| ! | ATTTTCGATCGATCTATGAA+TGG | + | chr7_2:1173857-1173876 | Msa1043230:intron | 30.0% |
| ! | TATTTCACTGTATTTAGCTC+TGG | - | chr7_2:1174119-1174138 | None:intergenic | 30.0% |
| !! | TTTAAAGGAAGCTGATTTGA+AGG | + | chr7_2:1174145-1174164 | Msa1043230:CDS | 30.0% |
| ATGGTTGTTGATATTGAACG+TGG | + | chr7_2:1173442-1173461 | Msa1043230:CDS | 35.0% | |
| !!! | CAATCGAAGGTATTTTACCA+AGG | + | chr7_2:1173546-1173565 | Msa1043230:intron | 35.0% |
| TATCATACTTGCAACAACTC+AGG | + | chr7_2:1174015-1174034 | Msa1043230:CDS | 35.0% | |
| CAACAACTCAGGAGAATTAA+TGG | + | chr7_2:1174026-1174045 | Msa1043230:CDS | 35.0% | |
| TGTTGCAAACGATGTTGAAA+AGG | + | chr7_2:1174214-1174233 | Msa1043230:CDS | 35.0% | |
| ATGGTTGTTGATATTGAACG+TGG | + | chr7_2:1173442-1173461 | Msa1043230:CDS | 35.0% | |
| !!! | CAATCGAAGGTATTTTACCA+AGG | + | chr7_2:1173546-1173565 | Msa1043230:intron | 35.0% |
| TATCATACTTGCAACAACTC+AGG | + | chr7_2:1174015-1174034 | Msa1043230:CDS | 35.0% | |
| CAACAACTCAGGAGAATTAA+TGG | + | chr7_2:1174026-1174045 | Msa1043230:CDS | 35.0% | |
| TGTTGCAAACGATGTTGAAA+AGG | + | chr7_2:1174214-1174233 | Msa1043230:CDS | 35.0% | |
| ! | AAAGTTGCTGCTGATGATGA+TGG | + | chr7_2:1173466-1173485 | Msa1043230:CDS | 40.0% |
| ! | TTGTTATTGTCGTTGCTCCT+AGG | + | chr7_2:1173503-1173522 | Msa1043230:CDS | 40.0% |
| GGAATCCTTCTATCAATCGA+AGG | + | chr7_2:1173533-1173552 | Msa1043230:CDS | 40.0% | |
| ! | TCAATCATGATTTGCGTCCT+TGG | - | chr7_2:1173566-1173585 | None:intergenic | 40.0% |
| TTGTTCAATAGGCAGTAACG+TGG | + | chr7_2:1173790-1173809 | Msa1043230:intron | 40.0% | |
| CTAAGCCTTAATTGGCTGAT+TGG | + | chr7_2:1173833-1173852 | Msa1043230:CDS | 40.0% | |
| CAATGGTCCATCATCAACTT+GGG | - | chr7_2:1174071-1174090 | None:intergenic | 40.0% | |
| ! | AAAGTTGCTGCTGATGATGA+TGG | + | chr7_2:1173466-1173485 | Msa1043230:CDS | 40.0% |
| ! | TTGTTATTGTCGTTGCTCCT+AGG | + | chr7_2:1173503-1173522 | Msa1043230:CDS | 40.0% |
| GGAATCCTTCTATCAATCGA+AGG | + | chr7_2:1173533-1173552 | Msa1043230:CDS | 40.0% | |
| ! | TCAATCATGATTTGCGTCCT+TGG | - | chr7_2:1173566-1173585 | None:intergenic | 40.0% |
| TTGTTCAATAGGCAGTAACG+TGG | + | chr7_2:1173790-1173809 | Msa1043230:intron | 40.0% | |
| CTAAGCCTTAATTGGCTGAT+TGG | + | chr7_2:1173833-1173852 | Msa1043230:CDS | 40.0% | |
| CAATGGTCCATCATCAACTT+GGG | - | chr7_2:1174071-1174090 | None:intergenic | 40.0% | |
| !! | TTGCTGCTGATGATGATGGT+GGG | + | chr7_2:1173470-1173489 | Msa1043230:CDS | 45.0% |
| TATTGTCGTTGCTCCTAGGA+TGG | + | chr7_2:1173507-1173526 | Msa1043230:CDS | 45.0% | |
| GAAGGATTCCTTTCCATCCT+AGG | - | chr7_2:1173523-1173542 | None:intergenic | 45.0% | |
| TGGAGTCTTAGCATACGAGA+AGG | + | chr7_2:1173877-1173896 | Msa1043230:intron | 45.0% | |
| TTGGGCGTGAGAATCAAATG+AGG | - | chr7_2:1174053-1174072 | None:intergenic | 45.0% | |
| CCAATGGTCCATCATCAACT+TGG | - | chr7_2:1174072-1174091 | None:intergenic | 45.0% | |
| !! | CCAAGTTGATGATGGACCAT+TGG | + | chr7_2:1174069-1174088 | Msa1043230:CDS | 45.0% |
| !! | TTGCTGCTGATGATGATGGT+GGG | + | chr7_2:1173470-1173489 | Msa1043230:CDS | 45.0% |
| TATTGTCGTTGCTCCTAGGA+TGG | + | chr7_2:1173507-1173526 | Msa1043230:CDS | 45.0% | |
| GAAGGATTCCTTTCCATCCT+AGG | - | chr7_2:1173523-1173542 | None:intergenic | 45.0% | |
| TGGAGTCTTAGCATACGAGA+AGG | + | chr7_2:1173877-1173896 | Msa1043230:intron | 45.0% | |
| TTGGGCGTGAGAATCAAATG+AGG | - | chr7_2:1174053-1174072 | None:intergenic | 45.0% | |
| CCAATGGTCCATCATCAACT+TGG | - | chr7_2:1174072-1174091 | None:intergenic | 45.0% | |
| !! | CCAAGTTGATGATGGACCAT+TGG | + | chr7_2:1174069-1174088 | Msa1043230:CDS | 45.0% |
| !! | GTTGCTGCTGATGATGATGG+TGG | + | chr7_2:1173469-1173488 | Msa1043230:CDS | 50.0% |
| TCGTTGCTCCTAGGATGGAA+AGG | + | chr7_2:1173512-1173531 | Msa1043230:CDS | 50.0% | |
| !! | TCTCACGCCCAAGTTGATGA+TGG | + | chr7_2:1174061-1174080 | Msa1043230:CDS | 50.0% |
| !! | GTTGCTGCTGATGATGATGG+TGG | + | chr7_2:1173469-1173488 | Msa1043230:CDS | 50.0% |
| TCGTTGCTCCTAGGATGGAA+AGG | + | chr7_2:1173512-1173531 | Msa1043230:CDS | 50.0% | |
| !! | TCTCACGCCCAAGTTGATGA+TGG | + | chr7_2:1174061-1174080 | Msa1043230:CDS | 50.0% |
| !! | GCTGATGATGATGGTGGGAG+TGG | + | chr7_2:1173475-1173494 | Msa1043230:CDS | 55.0% |
| AATAGGCAGTAACGTGGCGC+TGG | + | chr7_2:1173796-1173815 | Msa1043230:intron | 55.0% | |
| ATAGGCAGTAACGTGGCGCT+GGG | + | chr7_2:1173797-1173816 | Msa1043230:intron | 55.0% | |
| !! | GCTGATGATGATGGTGGGAG+TGG | + | chr7_2:1173475-1173494 | Msa1043230:CDS | 55.0% |
| AATAGGCAGTAACGTGGCGC+TGG | + | chr7_2:1173796-1173815 | Msa1043230:intron | 55.0% | |
| ATAGGCAGTAACGTGGCGCT+GGG | + | chr7_2:1173797-1173816 | Msa1043230:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 1173442 | 1174265 | 1173442 | ID=Msa1043230;Name=Msa1043230 |
| chr7_2 | mRNA | 1173442 | 1174265 | 1173442 | ID=Msa1043230-mRNA-1;Parent=Msa1043230;Name=Msa1043230-mRNA-1;_AED=0.40;_eAED=0.44;_QI=0|0|0|0.33|1|1|3|0|146 |
| chr7_2 | exon | 1173442 | 1173554 | 1173442 | ID=Msa1043230-mRNA-1:exon:205;Parent=Msa1043230-mRNA-1 |
| chr7_2 | exon | 1173805 | 1173854 | 1173805 | ID=Msa1043230-mRNA-1:exon:206;Parent=Msa1043230-mRNA-1 |
| chr7_2 | exon | 1173988 | 1174265 | 1173988 | ID=Msa1043230-mRNA-1:exon:207;Parent=Msa1043230-mRNA-1 |
| chr7_2 | CDS | 1173442 | 1173554 | 1173442 | ID=Msa1043230-mRNA-1:cds;Parent=Msa1043230-mRNA-1 |
| chr7_2 | CDS | 1173805 | 1173854 | 1173805 | ID=Msa1043230-mRNA-1:cds;Parent=Msa1043230-mRNA-1 |
| chr7_2 | CDS | 1173988 | 1174265 | 1173988 | ID=Msa1043230-mRNA-1:cds;Parent=Msa1043230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
