AlfalfaGEDB | Msa1044030

M. sativa cultivar zhongmu-4 / Msa1044030

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1044030	AES95624.2	69.925	266	32	8	4	258	1	229	2.63e-99	299

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1044030	sp\|Q8S9H7\|DIV_ANTMA	54.286	70	26	2	125	191	140	206	6.64e-15	76.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1044030	G7K884	69.925	266	32	8	4	258	1	229	1.26e-99	299

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TFs/TRs:

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Gene ID	Type	Classification
Msa1044030	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1044030	MtrunA17_Chr5g0409831	69.925	266	32	8	4	258	1	229	2.42e-103	299
Msa1044030	MtrunA17_Chr5g0409841	51.493	268	80	11	4	258	1	231	9.32e-72	219
Msa1044030	MtrunA17_Chr5g0440731	45.955	309	84	10	3	258	16	294	7.94e-69	214
Msa1044030	MtrunA17_Chr5g0440741	44.839	310	81	10	3	258	16	289	1.42e-64	203
Msa1044030	MtrunA17_Chr7g0241761	54.688	64	26	1	125	188	148	208	1.14e-16	78.2
Msa1044030	MtrunA17_Chr1g0210471	38.679	106	55	3	127	226	134	235	2.79e-16	75.9
Msa1044030	MtrunA17_Chr1g0162081	52.941	68	29	1	127	194	132	196	3.32e-16	76.6
Msa1044030	MtrunA17_Chr8g0364731	49.275	69	34	1	124	192	8	75	4.87e-16	73.9
Msa1044030	MtrunA17_Chr3g0104071	56.250	64	25	1	125	188	155	215	6.94e-16	75.9
Msa1044030	MtrunA17_Chr5g0429521	56.250	64	25	1	125	188	140	200	7.46e-16	75.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1044030	AT5G23650.1	43.636	110	54	3	125	234	128	229	1.06e-16	79.0
Msa1044030	AT5G58900.1	56.250	64	25	1	125	188	149	209	1.44e-16	77.8
Msa1044030	AT5G01200.1	58.065	62	23	1	127	188	156	214	1.72e-16	77.4
Msa1044030	AT2G38090.1	53.125	64	27	1	125	188	149	209	5.48e-16	76.3
Msa1044030	AT5G04760.1	50.000	72	31	2	125	196	107	173	2.55e-15	73.2
Msa1044030	AT5G08520.1	51.471	68	30	1	127	194	128	192	2.73e-15	74.3
Msa1044030	AT3G10580.2	49.296	71	30	2	127	197	105	169	7.41e-15	72.4
Msa1044030	AT3G10580.1	49.296	71	30	2	127	197	105	169	9.12e-15	72.8
Msa1044030	AT3G11280.2	46.914	81	36	2	112	188	118	195	3.46e-14	70.9
Msa1044030	AT3G11280.1	46.914	81	36	2	112	188	118	195	3.46e-14	70.9

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Find 45 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGAGGGCGTAAGGAAA+GGG	0.530997	7_2:-2203718	Msa1044030:CDS
AACCCAACAAATGTATCAGT+TGG	0.579773	7_2:-2199431	Msa1044030:CDS
AAGGTAGCTGTGTCTTGAGG+AGG	0.587982	7_2:+2199484	None:intergenic
ACAATTTAAGAGTACAAGAT+GGG	0.574479	7_2:-2201934	Msa1044030:CDS
AGGCCAACTGATACATTTGT+TGG	0.437602	7_2:+2199428	None:intergenic
AGTACAAGATGGGAGCTACA+TGG	0.581291	7_2:-2201924	Msa1044030:CDS
AGTACCGATTGGATAAAGCA+AGG	0.686649	7_2:+2199408	None:intergenic
ATATGTAGGTCGTTTCTACA+GGG	0.546671	7_2:-2199727	Msa1044030:intron
ATCATATTGATCTAAATGAG+AGG	0.625540	7_2:-2203926	Msa1044030:CDS
ATGTAGGTCGTTTCTACAGG+GGG	0.657825	7_2:-2199725	Msa1044030:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTAAGTATTCAATCTATCCT+TGG	-	chr7_2:2199697-2199716	Msa1044030:CDS	30.0%
AACAAACAGATACAAAGACT+GGG	+	chr7_2:2199882-2199901	None:intergenic	30.0%
AAACAAACAGATACAAAGAC+TGG	+	chr7_2:2199883-2199902	None:intergenic	30.0%
GGAATAATCTACAAAAATCG+AGG	-	chr7_2:2200215-2200234	Msa1044030:intron	30.0%
TTTCAATGTATGAGCATGTT+TGG	-	chr7_2:2200339-2200358	Msa1044030:intron	30.0%
AGAGAAGTGGAAAATAAGTA+GGG	+	chr7_2:2200365-2200384	None:intergenic	30.0%
AAGAGAAGTGGAAAATAAGT+AGG	+	chr7_2:2200366-2200385	None:intergenic	30.0%
TCACTAGGTTTATTCAGATT+TGG	+	chr7_2:2200641-2200660	None:intergenic	30.0%
TGGAACATATATATGTCACT+AGG	+	chr7_2:2200656-2200675	None:intergenic	30.0%
CAGTGCATATATCATGTAAA+AGG	+	chr7_2:2200815-2200834	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	2199339	2204050	2199339	ID=Msa1044030;Name=Msa1044030
chr7_2	mRNA	2199339	2204050	2199339	ID=Msa1044030-mRNA-1;Parent=Msa1044030;Name=Msa1044030-mRNA-1;_AED=0.06;_eAED=0.14;_QI=0\|0\|0\|0.8\|0.5\|0.4\|5\|0\|258
chr7_2	exon	2204046	2204050	2204046	ID=Msa1044030-mRNA-1:exon:546;Parent=Msa1044030-mRNA-1
chr7_2	exon	2203779	2203983	2203779	ID=Msa1044030-mRNA-1:exon:545;Parent=Msa1044030-mRNA-1
chr7_2	exon	2203693	2203762	2203693	ID=Msa1044030-mRNA-1:exon:544;Parent=Msa1044030-mRNA-1
chr7_2	exon	2201896	2201989	2201896	ID=Msa1044030-mRNA-1:exon:543;Parent=Msa1044030-mRNA-1
chr7_2	exon	2199339	2199741	2199339	ID=Msa1044030-mRNA-1:exon:542;Parent=Msa1044030-mRNA-1
chr7_2	CDS	2204046	2204050	2204046	ID=Msa1044030-mRNA-1:cds;Parent=Msa1044030-mRNA-1
chr7_2	CDS	2203779	2203983	2203779	ID=Msa1044030-mRNA-1:cds;Parent=Msa1044030-mRNA-1
chr7_2	CDS	2203693	2203762	2203693	ID=Msa1044030-mRNA-1:cds;Parent=Msa1044030-mRNA-1
chr7_2	CDS	2201896	2201989	2201896	ID=Msa1044030-mRNA-1:cds;Parent=Msa1044030-mRNA-1
chr7_2	CDS	2199339	2199741	2199339	ID=Msa1044030-mRNA-1:cds;Parent=Msa1044030-mRNA-1

Gene Sequence

>Msa1044030

ATGATGAATATGGAAGGAAGAAAAGTTCAACAATTACCTCATCATATTGATCTAAATGAGAGGACTTCAATTCAAGATCCCCCTGAGAATAGCATGGACCAACAACAAGAGAAACTTGACACTGATGTCGTTGAGTCGGGTTGTGCCGTCGCAATTCCAAACTCTAATGATGATAACATTGCGACTCTAAACTCTAATGATAATAACTTATACCAGAAGGAAGTCATGAAGCAAAGAGAGGGCGTAAGGAAAGGGTACATTGGACGGAAGGTGAACATAAAAGAAAGAAACATTAATGAAGATTTGAGTTCATACAATTTAAGAGTACAAGATGGGAGCTACATGGATAGAGCTCGAAATCAAATAGCCACTAAGTCGTTTCTACAGGGGGTGAAAAAACATGGTAGGGGACGTTGGAAAGATATTTCAAAAGAATTTGTGCGAACAAAAACTCCAACTCAAATTGCTAGTCATGCTCAAAAATACTTTGTTCACCACCAAACTGCCAAAGAAATAGAGAAGAAGAAAAAGAGAAGAAGTATTCACGACATAACCTTAAACAACAATGACACAGTAGTCACTCTCCCTCTTGAAAAACAACTTGAAATTCCTCCTCAAGACACAGCTACCTTAAATAACAAGGACAATGACAACTTGCAACAAACCCAACAAATGTATCAGTTGGCCTTGCTTTATCCAATCGGTACTGCACTTCCTGATATGAACAAACTTGAGAAGATGCGCAACTTGTTTAACTTGTTGGCAAAAGACCTTTGA

Protein sequence

>Msa1044030

MMNMEGRKVQQLPHHIDLNERTSIQDPPENSMDQQQEKLDTDVVESGCAVAIPNSNDDNIATLNSNDNNLYQKEVMKQREGVRKGYIGRKVNIKERNINEDLSSYNLRVQDGSYMDRARNQIATKSFLQGVKKHGRGRWKDISKEFVRTKTPTQIASHAQKYFVHHQTAKEIEKKKKRRSIHDITLNNNDTVVTLPLEKQLEIPPQDTATLNNKDNDNLQQTQQMYQLALLYPIGTALPDMNKLEKMRNLFNLLAKDL*