Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045290 | XP_013447966.1 | 72.273 | 220 | 19 | 1 | 1 | 178 | 1 | 220 | 2.35e-100 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045290 | A0A072TYR6 | 72.273 | 220 | 19 | 1 | 1 | 178 | 1 | 220 | 1.12e-100 | 298 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1045290 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045290 | MtrunA17_Chr7g0225091 | 72.273 | 220 | 19 | 1 | 1 | 178 | 1 | 220 | 2.16e-104 | 298 |
| Msa1045290 | MtrunA17_Chr7g0255511 | 63.675 | 234 | 29 | 2 | 1 | 178 | 1 | 234 | 5.57e-88 | 257 |
| Msa1045290 | MtrunA17_Chr7g0225121 | 56.410 | 234 | 24 | 5 | 1 | 178 | 1 | 212 | 5.89e-71 | 213 |
| Msa1045290 | MtrunA17_Chr7g0222361 | 55.446 | 202 | 33 | 3 | 34 | 178 | 1 | 202 | 1.02e-66 | 202 |
| Msa1045290 | MtrunA17_Chr7g0222371 | 76.404 | 89 | 21 | 0 | 19 | 107 | 1 | 89 | 9.18e-48 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAATGGAGAAATTTCTT+TGG | 0.293893 | 7_2:+4600606 | None:intergenic |
| CTCCTTCCTCTTGTTGCACA+TGG | 0.338528 | 7_2:+4600786 | None:intergenic |
| TATATATAAATCAAAGCTTA+AGG | 0.356106 | 7_2:+4600135 | None:intergenic |
| TGGTGCATTGCAACATATTC+CGG | 0.362580 | 7_2:-4600321 | Msa1045290:CDS |
| CTTAAGGGTCAAATCAATCT+TGG | 0.396784 | 7_2:+4600151 | None:intergenic |
| AGTAGACAATTTGAAGATGA+TGG | 0.466141 | 7_2:-4600746 | Msa1045290:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7_2:-4600511 | Msa1045290:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7_2:-4600579 | Msa1045290:CDS |
| TGAAGTTGGTAAAGTTTCAG+AGG | 0.484639 | 7_2:-4600234 | Msa1045290:CDS |
| ACTATGTCAAAGATTGAAGT+TGG | 0.488225 | 7_2:-4600248 | Msa1045290:CDS |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | 7_2:-4600204 | Msa1045290:CDS |
| CAAGCGAAAGGCAATTGTGC+TGG | 0.510108 | 7_2:-4600704 | Msa1045290:CDS |
| GAAGTTGGTAAAGTTTCAGA+GGG | 0.513957 | 7_2:-4600233 | Msa1045290:CDS |
| ATATATAAATCAAAGCTTAA+GGG | 0.520066 | 7_2:+4600136 | None:intergenic |
| GGAGATAGATGAAGCGAATG+AGG | 0.566081 | 7_2:-4600300 | Msa1045290:CDS |
| TCATTCGCTTCATCTATCTC+CGG | 0.569516 | 7_2:+4600302 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7_2:-4600518 | Msa1045290:CDS |
| CTGATGCCATGTGCAACAAG+AGG | 0.598560 | 7_2:-4600792 | None:intergenic |
| TAGAATTCTGAGTTTCAACA+TGG | 0.602831 | 7_2:+4600674 | None:intergenic |
| TGCCATGTGCAACAAGAGGA+AGG | 0.602996 | 7_2:-4600788 | None:intergenic |
| GTTGGTAAAGTTTCAGAGGG+AGG | 0.624522 | 7_2:-4600230 | Msa1045290:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7_2:-4600183 | Msa1045290:CDS |
| GTGCAACAAGAGGAAGGAGA+AGG | 0.635140 | 7_2:-4600782 | Msa1045290:CDS |
| GAACAATGAGAACAAGCGAA+AGG | 0.645636 | 7_2:-4600716 | Msa1045290:CDS |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7_2:-4600508 | Msa1045290:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.664402 | 7_2:+4600502 | None:intergenic |
| AGAATTCTGAGTTTCAACAT+GGG | 0.673130 | 7_2:+4600675 | None:intergenic |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7_2:-4600180 | Msa1045290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | + | chr7_2:4600326-4600345 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | - | chr7_2:4600331-4600350 | Msa1045290:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | + | chr7_2:4600340-4600359 | None:intergenic | 25.0% |
| !! | TTTTATGCAGGAAATTTGTA+TGG | - | chr7_2:4600588-4600607 | Msa1045290:CDS | 25.0% |
| AGTAGACAATTTGAAGATGA+TGG | - | chr7_2:4600183-4600202 | Msa1045290:CDS | 30.0% | |
| AGAATTCTGAGTTTCAACAT+GGG | + | chr7_2:4600257-4600276 | None:intergenic | 30.0% | |
| TAGAATTCTGAGTTTCAACA+TGG | + | chr7_2:4600258-4600277 | None:intergenic | 30.0% | |
| AGTCTCAATAAAAAGATCCA+AGG | - | chr7_2:4600472-4600491 | Msa1045290:intron | 30.0% | |
| GTCTCAATAAAAAGATCCAA+GGG | - | chr7_2:4600473-4600492 | Msa1045290:intron | 30.0% | |
| AACAATATGATTCAACATGC+AGG | - | chr7_2:4600555-4600574 | Msa1045290:CDS | 30.0% | |
| ACAATATGATTCAACATGCA+GGG | - | chr7_2:4600556-4600575 | Msa1045290:CDS | 30.0% | |
| ACTATGTCAAAGATTGAAGT+TGG | - | chr7_2:4600681-4600700 | Msa1045290:CDS | 30.0% | |
| ! | CTTCTTCAGCAACATCTTTT+TGG | + | chr7_2:4600288-4600307 | None:intergenic | 35.0% |
| TTGGATTCATTGTTGATCAC+AGG | - | chr7_2:4600350-4600369 | Msa1045290:CDS | 35.0% | |
| AACCATCACTCCATTTCATA+TGG | - | chr7_2:4600506-4600525 | Msa1045290:CDS | 35.0% | |
| ACCATCACTCCATTTCATAT+GGG | - | chr7_2:4600507-4600526 | Msa1045290:CDS | 35.0% | |
| GGGTGTGGATATCAATATAA+TGG | - | chr7_2:4600528-4600547 | Msa1045290:CDS | 35.0% | |
| !! | TGAAGTTGGTAAAGTTTCAG+AGG | - | chr7_2:4600695-4600714 | Msa1045290:CDS | 35.0% |
| !! | GAAGTTGGTAAAGTTTCAGA+GGG | - | chr7_2:4600696-4600715 | Msa1045290:CDS | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | - | chr7_2:4600725-4600744 | Msa1045290:CDS | 35.0% |
| CTTAAGGGTCAAATCAATCT+TGG | + | chr7_2:4600781-4600800 | None:intergenic | 35.0% | |
| GAACAATGAGAACAAGCGAA+AGG | - | chr7_2:4600213-4600232 | Msa1045290:CDS | 40.0% | |
| ! | ATGGTTTGAGACTTATCCCT+TGG | + | chr7_2:4600492-4600511 | None:intergenic | 40.0% |
| CCATCACTCCATTTCATATG+GGG | - | chr7_2:4600508-4600527 | Msa1045290:CDS | 40.0% | |
| TATCCACACCCCATATGAAA+TGG | + | chr7_2:4600519-4600538 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | - | chr7_2:4600576-4600595 | Msa1045290:CDS | 40.0% |
| TGGTGCATTGCAACATATTC+CGG | - | chr7_2:4600608-4600627 | Msa1045290:CDS | 40.0% | |
| TCATTCGCTTCATCTATCTC+CGG | + | chr7_2:4600630-4600649 | None:intergenic | 40.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | - | chr7_2:4600411-4600430 | Msa1045290:intron | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | - | chr7_2:4600418-4600437 | Msa1045290:intron | 45.0% | |
| CCCCATATGAAATGGAGTGA+TGG | + | chr7_2:4600511-4600530 | None:intergenic | 45.0% | |
| ACTCCATTTCATATGGGGTG+TGG | - | chr7_2:4600513-4600532 | Msa1045290:CDS | 45.0% | |
| GGAGATAGATGAAGCGAATG+AGG | - | chr7_2:4600629-4600648 | Msa1045290:CDS | 45.0% | |
| ! | GTTGGTAAAGTTTCAGAGGG+AGG | - | chr7_2:4600699-4600718 | Msa1045290:CDS | 45.0% |
| GGAAGACAAAGAGACTCAAG+AGG | - | chr7_2:4600746-4600765 | Msa1045290:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | - | chr7_2:4600749-4600768 | Msa1045290:CDS | 45.0% | |
| GTGCAACAAGAGGAAGGAGA+AGG | - | chr7_2:4600147-4600166 | Msa1045290:CDS | 50.0% | |
| CAAGCGAAAGGCAATTGTGC+TGG | - | chr7_2:4600225-4600244 | Msa1045290:CDS | 50.0% | |
| ACTAGTCCACAGGCATTAGG+TGG | - | chr7_2:4600421-4600440 | Msa1045290:intron | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | + | chr7_2:4600430-4600449 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 4600145 | 4600806 | 4600145 | ID=Msa1045290;Name=Msa1045290 |
| chr7_2 | mRNA | 4600145 | 4600806 | 4600145 | ID=Msa1045290-mRNA-1;Parent=Msa1045290;Name=Msa1045290-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|1|1|2|0|178 |
| chr7_2 | exon | 4600500 | 4600806 | 4600500 | ID=Msa1045290-mRNA-1:exon:1084;Parent=Msa1045290-mRNA-1 |
| chr7_2 | exon | 4600145 | 4600374 | 4600145 | ID=Msa1045290-mRNA-1:exon:1083;Parent=Msa1045290-mRNA-1 |
| chr7_2 | CDS | 4600500 | 4600806 | 4600500 | ID=Msa1045290-mRNA-1:cds;Parent=Msa1045290-mRNA-1 |
| chr7_2 | CDS | 4600145 | 4600374 | 4600145 | ID=Msa1045290-mRNA-1:cds;Parent=Msa1045290-mRNA-1 |
| Gene Sequence |
| Protein sequence |
