AlfalfaGEDB | Msa1050860

M. sativa cultivar zhongmu-4 / Msa1050860

KEGG: K09264
GO: -
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1050860	XP_003621521.1	98.047	256	5	0	1	256	1	256	0.0	518

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1050860	sp\|D7SMN6\|FULL_VITVI	59.144	257	90	6	1	254	1	245	2.75e-86	259

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1050860	G7KR16	98.047	256	5	0	1	256	1	256	0.0	518

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TFs/TRs:

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Gene ID	Type	Classification
Msa1050860	TF	MADS-MIKC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1050860	MtrunA17_Chr7g0220121	98.047	256	5	0	1	256	1	256	0.0	518
Msa1050860	MtrunA17_Chr8g0366231	65.476	168	56	1	1	166	1	168	5.56e-73	223
Msa1050860	MtrunA17_Chr5g0420081	54.911	224	84	6	1	219	1	212	9.31e-73	222
Msa1050860	MtrunA17_Chr4g0061691	51.373	255	92	5	1	251	1	227	1.01e-72	222
Msa1050860	MtrunA17_Chr6g0455511	39.852	271	122	8	1	251	1	250	1.29e-52	171
Msa1050860	MtrunA17_Chr7g0220111	38.760	258	142	4	1	251	1	249	3.61e-51	167
Msa1050860	MtrunA17_Chr3g0122251	50.000	176	83	2	1	171	1	176	1.81e-49	163
Msa1050860	MtrunA17_Chr5g0420091	42.326	215	106	3	1	198	1	214	2.88e-49	161
Msa1050860	MtrunA17_Chr8g0351051	41.045	268	124	9	1	251	1	251	5.57e-49	162
Msa1050860	MtrunA17_Chr4g0061671	48.503	167	84	2	1	166	1	166	4.12e-47	155

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1050860	AT5G60910.1	51.181	254	102	7	1	251	1	235	2.27e-73	224
Msa1050860	AT1G69120.2	63.128	179	63	2	1	176	1	179	2.58e-72	221
Msa1050860	AT1G69120.1	63.128	179	63	2	1	176	1	179	5.42e-72	221
Msa1050860	AT1G26310.1	50.644	233	98	5	1	222	1	227	4.44e-69	214
Msa1050860	AT3G30260.1	57.895	152	62	1	1	150	1	152	4.16e-54	175
Msa1050860	AT1G24260.1	53.529	170	75	2	1	166	1	170	1.27e-50	166
Msa1050860	AT1G24260.2	52.632	171	76	2	1	166	1	171	1.66e-49	163
Msa1050860	AT5G15800.1	48.066	181	92	1	1	179	1	181	2.07e-48	160
Msa1050860	AT5G15800.2	48.066	181	92	1	1	179	1	181	2.27e-48	161
Msa1050860	AT3G02310.1	41.016	256	140	3	1	251	1	250	3.24e-48	160

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Find 58 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAATTCATCATGGGAA+GGG	0.551499	7_2:+17013347	Msa1050860:exon
AAACAATTCATCATGGGAAG+GGG	0.571511	7_2:+17013348	Msa1050860:exon
AAACAGCATGGAAGACATTT+TGG	0.233989	7_2:+17017163	Msa1050860:intron
AAAGCGTATCCGAACAAGAA+AGG	0.507445	7_2:+17018000	Msa1050860:CDS
AAATACCTGAGATTTAGAGA+AGG	0.668458	7_2:-17018989	None:intergenic
AAGCCTTAGAGAGGAACTTA+AGG	0.466792	7_2:+17017440	Msa1050860:CDS
AATTCATCATGGGAAGGGGT+AGG	0.529352	7_2:+17013352	Msa1050860:exon
AGAGAAGGAACCAGTCTTTG+TGG	0.527257	7_2:-17018974	None:intergenic
AGATGATGGAGCATCCATGG+TGG	0.769944	7_2:-17019614	None:intergenic
AGATGGATTGATTCATGACT+TGG	0.494556	7_2:-17018159	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACAATTCATCATGGGA+AGG	+	chr7_2:17013346-17013365	Msa1050860:exon	30.0%
AAAACAATTCATCATGGGAA+GGG	+	chr7_2:17013347-17013366	Msa1050860:exon	30.0%
TGTATCTCTTGTATCATCTT+AGG	+	chr7_2:17013847-17013866	Msa1050860:intron	30.0%
TCATAGGCCTTTAAATATGT+TGG	+	chr7_2:17013945-17013964	Msa1050860:intron	30.0%
TAGATAGATAGAAAAAGGGT+TGG	-	chr7_2:17014130-17014149	None:intergenic	30.0%
AACACTTCAAAAAAGCAACA+AGG	-	chr7_2:17014182-17014201	None:intergenic	30.0%
TATCTGGTACTTCATCTTTA+AGG	-	chr7_2:17014613-17014632	None:intergenic	30.0%
ATCTATGAATAACCTAAGCA+AGG	+	chr7_2:17015079-17015098	Msa1050860:intron	30.0%
TGTATGTATAGTCCTTAGTT+AGG	-	chr7_2:17015113-17015132	None:intergenic	30.0%
TGACACTTATGTAATAACCT+AGG	-	chr7_2:17015468-17015487	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	17013339	17019646	17013339	ID=Msa1050860;Name=Msa1050860
chr7_2	mRNA	17013339	17019646	17013339	ID=Msa1050860-mRNA-1;Parent=Msa1050860;Name=Msa1050860-mRNA-1;_AED=0.16;_eAED=0.17;_QI=21\|0.14\|0.12\|1\|1\|1\|8\|0\|256
chr7_2	exon	17013339	17013544	17013339	ID=Msa1050860-mRNA-1:exon:3798;Parent=Msa1050860-mRNA-1
chr7_2	exon	17017169	17017241	17017169	ID=Msa1050860-mRNA-1:exon:3799;Parent=Msa1050860-mRNA-1
chr7_2	exon	17017397	17017461	17017397	ID=Msa1050860-mRNA-1:exon:3800;Parent=Msa1050860-mRNA-1
chr7_2	exon	17017922	17018021	17017922	ID=Msa1050860-mRNA-1:exon:3801;Parent=Msa1050860-mRNA-1
chr7_2	exon	17018157	17018198	17018157	ID=Msa1050860-mRNA-1:exon:3802;Parent=Msa1050860-mRNA-1
chr7_2	exon	17018542	17018580	17018542	ID=Msa1050860-mRNA-1:exon:3803;Parent=Msa1050860-mRNA-1
chr7_2	exon	17018857	17019005	17018857	ID=Msa1050860-mRNA-1:exon:3804;Parent=Msa1050860-mRNA-1
chr7_2	exon	17019529	17019646	17019529	ID=Msa1050860-mRNA-1:exon:3805;Parent=Msa1050860-mRNA-1
chr7_2	five_prime_UTR	17013339	17013359	17013339	ID=Msa1050860-mRNA-1:five_prime_utr;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17013360	17013544	17013360	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17017169	17017241	17017169	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17017397	17017461	17017397	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17017922	17018021	17017922	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17018157	17018198	17018157	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17018542	17018580	17018542	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17018857	17019005	17018857	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1
chr7_2	CDS	17019529	17019646	17019529	ID=Msa1050860-mRNA-1:cds;Parent=Msa1050860-mRNA-1

Gene Sequence

>Msa1050860

ATGGGAAGGGGTAGGGTTCAGCTGAAACGGATCGAGAACAAAACAAGCCAACAAGTAACTTTTTTCAAAAGAAGAACTGGACTTCTCAAGAAAGCTAATGAAATTTCAGTTCTTTGTGATGCTCAAGTTGCTTTGATTATGTTCTCCACCAAAGGAAAACTTTTTGAGTATTCCTCTGCACCAAGCATGGAAGACATTTTGGAACGATATGAGAGACAAAATCATACGGAACTTACTGGAGCTACCAATGAAACACAGGGAAATTGGTCCTTTGAATACATGAAGCTCACTGCTAAAGTTCAAGCCTTAGAGAGGAACTTAAGGAACTTTGTGGGAAATGATCTGGACCCCTTGAGTGTGAAAGAGCTTCAGAGTTTGGAGCAACAGCTTGACACATCGCTAAAGCGTATCCGAACAAGAAAGAACCAAGTCATGAATCAATCCATCTCAGAGCTGCACAAAAGGGCAAGGACATTGCAGGAGCAAAACAGCAAGCTAGCAAAGACAAAGGAGAAAGAGAAGACAGTGAGTGAACATCCTCAAAGATGGCTACAAACCATAGGCATAGGGCAATGTTCATCCACCCTCAACTTAATCTGTCAACCAGAGGTGCTACCACCACCACAAAGACTGGTTCCTTCTCTAAATCTCAGTGGGACTTTACAAGCAAGAGGGTCATTGGAATTTGAAGAAGCAGGTGAAGCTCAAACTGTTCCTAGAAGCAATCATAGTCTCATGCCACCATGGATGCTCCATCATCTCACCAACTAG

Protein sequence

>Msa1050860

MGRGRVQLKRIENKTSQQVTFFKRRTGLLKKANEISVLCDAQVALIMFSTKGKLFEYSSAPSMEDILERYERQNHTELTGATNETQGNWSFEYMKLTAKVQALERNLRNFVGNDLDPLSVKELQSLEQQLDTSLKRIRTRKNQVMNQSISELHKRARTLQEQNSKLAKTKEKEKTVSEHPQRWLQTIGIGQCSSTLNLICQPEVLPPPQRLVPSLNLSGTLQARGSLEFEEAGEAQTVPRSNHSLMPPWMLHHLTN*