AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1051580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1051580 MtrunA17_Chr7g0220931 93.684 380 16 4 1 378 1 374 0.0 541
Msa1051580 MtrunA17_Chr6g0456881 46.761 355 106 17 73 364 67 401 5.12e-67 218
Msa1051580 MtrunA17_Chr1g0205131 35.677 384 162 15 41 356 27 393 2.37e-44 157
Msa1051580 MtrunA17_Chr4g0062691 33.459 266 141 11 5 245 14 268 2.37e-26 108
Msa1051580 MtrunA17_Chr8g0376571 34.409 186 79 5 116 266 57 234 6.38e-19 88.2
Msa1051580 MtrunA17_Chr2g0316391 53.846 65 30 0 115 179 15 79 5.07e-17 80.9
Msa1051580 MtrunA17_Chr2g0325171 51.562 64 31 0 115 178 19 82 1.07e-16 80.5
Msa1051580 MtrunA17_Chr4g0040921 52.308 65 31 0 115 179 17 81 1.30e-16 80.1
Msa1051580 MtrunA17_Chr8g0360441 52.308 65 31 0 115 179 49 113 1.41e-16 80.9
Msa1051580 MtrunA17_Chr6g0454781 47.561 82 41 1 119 200 48 127 7.30e-16 77.8
Msa1051580 MtrunA17_Chr7g0219461 51.667 60 29 0 119 178 56 115 1.63e-14 73.9
Msa1051580 MtrunA17_Chr4g0061561 48.333 60 31 0 119 178 56 115 4.35e-13 69.3
Msa1051580 MtrunA17_Chr3g0086961 48.333 60 31 0 119 178 56 115 2.12e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1051580 AT1G67260.2 37.079 267 133 10 73 312 47 305 2.66e-34 130
Msa1051580 AT1G67260.3 37.079 267 133 10 73 312 47 305 2.66e-34 130
Msa1051580 AT1G67260.1 37.079 267 133 10 73 312 47 305 2.74e-34 130
Msa1051580 AT1G68800.2 53.982 113 43 4 120 229 112 218 7.66e-25 104
Msa1051580 AT1G68800.1 53.982 113 43 4 120 229 112 218 8.77e-25 104
Msa1051580 AT3G18550.2 38.562 153 70 3 113 241 141 293 1.25e-21 96.3
Msa1051580 AT3G18550.3 37.580 157 70 4 113 241 141 297 3.52e-20 92.0
Msa1051580 AT3G18550.1 37.580 157 70 4 113 241 141 297 4.78e-20 91.7
Msa1051580 AT3G18550.4 37.580 157 70 4 113 241 141 297 4.78e-20 91.7
Msa1051580 AT4G18390.1 35.374 147 89 3 103 248 27 168 5.07e-18 85.1
Msa1051580 AT4G18390.2 35.374 147 89 3 103 248 27 168 5.07e-18 85.1
Msa1051580 AT1G30210.2 53.968 63 29 0 116 178 46 108 1.61e-16 80.1
Msa1051580 AT1G30210.1 53.968 63 29 0 116 178 46 108 1.61e-16 80.1
Msa1051580 AT1G53230.1 51.515 66 32 0 115 180 44 109 2.20e-16 80.5
Msa1051580 AT1G53230.2 51.515 66 32 0 115 180 44 109 2.20e-16 80.5
Msa1051580 AT3G15030.2 52.308 65 31 0 115 179 40 104 2.83e-16 80.1
Msa1051580 AT3G15030.4 52.308 65 31 0 115 179 40 104 2.83e-16 80.1
Msa1051580 AT3G15030.3 52.308 65 31 0 115 179 40 104 2.83e-16 80.1
Msa1051580 AT3G15030.1 52.308 65 31 0 115 179 40 104 2.83e-16 80.1
Msa1051580 AT2G31070.1 51.562 64 31 0 115 178 24 87 4.73e-16 79.3
Msa1051580 AT3G02150.1 53.333 60 28 0 119 178 73 132 1.12e-14 73.9
Msa1051580 AT3G02150.2 53.333 60 28 0 119 178 73 132 1.79e-14 74.3
Msa1051580 AT5G60970.1 46.667 60 32 0 119 178 62 121 9.30e-13 69.3
Msa1051580 AT5G08070.2 45.000 60 33 0 119 178 32 91 2.51e-11 63.5
Msa1051580 AT5G08070.1 45.000 60 33 0 119 178 32 91 2.51e-11 63.5

Find 81 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCAACTTATTGCTCTTT+AGG 0.129653 7_2:+18954786 Msa1051580:CDS
ATCCTGATACACTAACAAAT+TGG 0.193845 7_2:+18955012 Msa1051580:CDS
AATAAGCCTTCATATTTATC+TGG 0.194825 7_2:-18954452 None:intergenic
GCCCAATTTGTTAGTGTATC+AGG 0.208804 7_2:-18955014 None:intergenic
CTCCAATGTTATCACCAAAT+TGG 0.217754 7_2:+18955741 Msa1051580:CDS
GTTAGTGTATCAGGATTAAT+TGG 0.223236 7_2:-18955005 None:intergenic
GGTATATAGCTATGTTCTTA+TGG 0.234961 7_2:-18954685 None:intergenic
CTTCGAAGACGAAGGATTCT+AGG 0.286350 7_2:+18955447 Msa1051580:CDS
CACGGATATTAAATATTTAG+AGG 0.316758 7_2:-18954626 None:intergenic
AGCAAGAACAACATAGTTTC+TGG 0.320896 7_2:+18955320 Msa1051580:CDS
TTCGATCTTCAAGACATGTT+AGG 0.334610 7_2:+18955224 Msa1051580:CDS
TTTGAAGTTTCATCCTCATT+AGG 0.336904 7_2:-18955584 None:intergenic
TATTTAATATCCGTGTAAAA+AGG 0.339349 7_2:+18954634 Msa1051580:five_prime_UTR
TTATTATTAGCATCCCAATT+TGG 0.341400 7_2:-18955755 None:intergenic
TCATGACAATCACTATTATT+AGG 0.360860 7_2:-18955716 None:intergenic
TCAGGGGTAAGGAAAGTAAA+AGG 0.368203 7_2:-18954861 None:intergenic
AATATCAGCTAATATCGATT+TGG 0.375751 7_2:-18956024 None:intergenic
ATTATATGGAAAGTGAGATT+TGG 0.383770 7_2:-18954500 None:intergenic
GTATATAGCTATGTTCTTAT+GGG 0.394932 7_2:-18954684 None:intergenic
TTCTTAGCCGGTTTCTTCAC+CGG 0.405185 7_2:-18955119 None:intergenic
GAGCCATTCAAGTGTGTTGC+TGG 0.409129 7_2:-18955258 None:intergenic
TTCAAATCTTTGGAAAATCA+TGG 0.409744 7_2:+18955983 Msa1051580:CDS
TCCAATGTTATCACCAAATT+GGG 0.411724 7_2:+18955742 Msa1051580:CDS
AATGAACTACTTACTAGTTA+AGG 0.419311 7_2:-18954602 None:intergenic
CTATGCCTGTCTTTCTTAGC+CGG 0.420461 7_2:-18955131 None:intergenic
TAGGTGAAGGGATTTCATTT+TGG 0.421354 7_2:-18954526 None:intergenic
TCTTGATATTGCAGAAAAGT+TGG 0.424180 7_2:-18955804 None:intergenic
GATTGATTACTTAACTCAAA+TGG 0.427355 7_2:-18954752 None:intergenic
TCCTGATACACTAACAAATT+GGG 0.434497 7_2:+18955013 Msa1051580:CDS
TCGATCTTCAAGACATGTTA+GGG 0.442491 7_2:+18955225 Msa1051580:CDS
TTGTTGTGAAACAAGAAACT+AGG 0.471445 7_2:+18954721 Msa1051580:five_prime_UTR
TGAAAGAAATTGATGATGAT+AGG 0.471978 7_2:-18954545 None:intergenic
TTCATCTCCTTCATCGGACG+AGG 0.483567 7_2:-18955378 None:intergenic
TCTTCTTCCTCGTCCGATGA+AGG 0.488840 7_2:+18955371 Msa1051580:CDS
TGATGAGTTGTTTGTGTGTA+GGG 0.490224 7_2:-18954975 None:intergenic
AAAGAAACCGGTGAAGAAAC+CGG 0.491500 7_2:+18955112 Msa1051580:CDS
GGTGTGATGGACAAGGCTCA+TGG 0.495803 7_2:-18954430 None:intergenic
AATAACAATGATGCTGTCAC+TGG 0.496363 7_2:+18955776 Msa1051580:CDS
AAATTTGCTCACAAAGAAAC+CGG 0.498565 7_2:+18955100 Msa1051580:CDS
CATTAGGCATCAATTGATGA+AGG 0.499616 7_2:-18955568 None:intergenic
ATCTTCTTCATCTCCTTCAT+CGG 0.500031 7_2:-18955384 None:intergenic
CCTAAAGAGCAATAAGTTGA+AGG 0.500471 7_2:-18954786 None:intergenic
ATAAGCCTTCATATTTATCT+GGG 0.502281 7_2:-18954451 None:intergenic
AGCAAGATTCACACATCTCA+AGG 0.503482 7_2:+18955152 Msa1051580:CDS
TCAAGAACGAATCTATCATC+AGG 0.510472 7_2:+18955821 Msa1051580:CDS
TGTTGTGAAACAAGAAACTA+GGG 0.521317 7_2:+18954722 Msa1051580:five_prime_UTR
TGTGATGAAGAAGATGAGTT+AGG 0.521777 7_2:-18954828 None:intergenic
TCAAATCTTTGGAAAATCAT+GGG 0.526493 7_2:+18955984 Msa1051580:CDS
GTGTGATGGACAAGGCTCAT+GGG 0.528245 7_2:-18954429 None:intergenic
GAACTTCCTCGCGATCTCGA+TGG 0.528472 7_2:-18955201 None:intergenic
TCATATTTATCTGGGTGTGA+TGG 0.528856 7_2:-18954443 None:intergenic
AAGCCAGCAACACACTTGAA+TGG 0.532128 7_2:+18955255 Msa1051580:CDS
TCATCAATTGATGCCTAATG+AGG 0.536962 7_2:+18955571 Msa1051580:CDS
TTCGAAGACGAAGGATTCTA+GGG 0.537014 7_2:+18955448 Msa1051580:CDS
AGAAAGCAAGAGCAAGAACA+AGG 0.538860 7_2:+18955471 Msa1051580:CDS
TGATATCTTTAACTTCATTG+AGG 0.548363 7_2:+18955622 Msa1051580:CDS
TAGGGAACAGAAATTGCTGC+TGG 0.557496 7_2:-18954957 None:intergenic
TCCCAATTTGGTGATAACAT+TGG 0.563066 7_2:-18955743 None:intergenic
TTAGGATTATAACAAGGGTA+AGG 0.565101 7_2:-18954810 None:intergenic
AGAAACCGGCTAAGAAAGAC+AGG 0.565124 7_2:+18955126 Msa1051580:CDS
GATGAGTTAGGATTATAACA+AGG 0.570713 7_2:-18954816 None:intergenic
TCACACCCAGATAAATATGA+AGG 0.575626 7_2:+18954446 Msa1051580:five_prime_UTR
ATGATGAGTTGTTTGTGTGT+AGG 0.581066 7_2:-18954976 None:intergenic
GGGTAAGGAAAGTAAAAGGA+GGG 0.582814 7_2:-18954857 None:intergenic
CAGCTAATATCGATTTGGAC+TGG 0.588709 7_2:-18956019 None:intergenic
AAATTGATGATGATAGGTGA+AGG 0.588891 7_2:-18954539 None:intergenic
GGGGTAAGGAAAGTAAAAGG+AGG 0.592199 7_2:-18954858 None:intergenic
AATTGATGATGATAGGTGAA+GGG 0.600381 7_2:-18954538 None:intergenic
ATGAGTTAGGATTATAACAA+GGG 0.601724 7_2:-18954815 None:intergenic
GACTTTCCATCGAGATCGCG+AGG 0.608900 7_2:+18955195 Msa1051580:CDS
GATGGACAAGGCTCATGGGT+TGG 0.611574 7_2:-18954425 None:intergenic
CTCAAGGGTTGAGAGACAGA+AGG 0.613193 7_2:+18955168 Msa1051580:CDS
GAAAGCAAGAGCAAGAACAA+GGG 0.614345 7_2:+18955472 Msa1051580:CDS
TTATCTGGGTGTGATGGACA+AGG 0.614983 7_2:-18954437 None:intergenic
GAAAGAATCTTCGAAGACGA+AGG 0.618129 7_2:+18955439 Msa1051580:CDS
TCAAGGGTTGAGAGACAGAA+GGG 0.623957 7_2:+18955169 Msa1051580:CDS
AATCTTTGGAAAATCATGGG+AGG 0.624471 7_2:+18955987 Msa1051580:CDS
GCAAGATTCACACATCTCAA+GGG 0.635283 7_2:+18955153 Msa1051580:CDS
TTAAGGACTATAGTGAACCT+AGG 0.642726 7_2:-18954585 None:intergenic
GTTTCTGGCGATGACGACGA+TGG 0.662203 7_2:+18955335 Msa1051580:CDS
ATGTTGAAACAAGATCTAAG+TGG 0.698091 7_2:+18955056 Msa1051580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAATTAATTAACAAAA+AGG + chr7_2:18955886-18955905 Msa1051580:intron 10.0%
!! CATAAAATTAATTAACAAAA+AGG + chr7_2:18955886-18955905 Msa1051580:intron 10.0%
!! TAAAAATAGAAAGATGAAAA+GGG + chr7_2:18955412-18955431 Msa1051580:CDS 15.0%
!!! TTTTTAGGTTTAAACTTTTT+AGG + chr7_2:18955958-18955977 Msa1051580:intron 15.0%
!!! TTTTAGGTTTAAACTTTTTA+GGG + chr7_2:18955959-18955978 Msa1051580:intron 15.0%
!! TAAAAATAGAAAGATGAAAA+GGG + chr7_2:18955412-18955431 Msa1051580:CDS 15.0%
!!! TTTTTAGGTTTAAACTTTTT+AGG + chr7_2:18955958-18955977 Msa1051580:intron 15.0%
!!! TTTTAGGTTTAAACTTTTTA+GGG + chr7_2:18955959-18955978 Msa1051580:intron 15.0%
!!! TTTCATTTTGGACAATTATA+TGG - chr7_2:18954517-18954536 None:intergenic 20.0%
!! TATTTAATATCCGTGTAAAA+AGG + chr7_2:18954634-18954653 Msa1051580:five_prime_UTR 20.0%
!! CTAAAAATAGAAAGATGAAA+AGG + chr7_2:18955411-18955430 Msa1051580:CDS 20.0%
!!! TTGTGATACTTACAATTTTT+AGG + chr7_2:18955943-18955962 Msa1051580:intron 20.0%
!!! TTTCATTTTGGACAATTATA+TGG - chr7_2:18954517-18954536 None:intergenic 20.0%
!! TATTTAATATCCGTGTAAAA+AGG + chr7_2:18954634-18954653 Msa1051580:five_prime_UTR 20.0%
!! CTAAAAATAGAAAGATGAAA+AGG + chr7_2:18955411-18955430 Msa1051580:CDS 20.0%
!!! TTGTGATACTTACAATTTTT+AGG + chr7_2:18955943-18955962 Msa1051580:intron 20.0%
! ATAAGCCTTCATATTTATCT+GGG - chr7_2:18954454-18954473 None:intergenic 25.0%
! AATAAGCCTTCATATTTATC+TGG - chr7_2:18954455-18954474 None:intergenic 25.0%
! ATTATATGGAAAGTGAGATT+TGG - chr7_2:18954503-18954522 None:intergenic 25.0%
!!! TGAAAGAAATTGATGATGAT+AGG - chr7_2:18954548-18954567 None:intergenic 25.0%
! AATGAACTACTTACTAGTTA+AGG - chr7_2:18954605-18954624 None:intergenic 25.0%
! CACGGATATTAAATATTTAG+AGG - chr7_2:18954629-18954648 None:intergenic 25.0%
! GTATATAGCTATGTTCTTAT+GGG - chr7_2:18954687-18954706 None:intergenic 25.0%
! GATTGATTACTTAACTCAAA+TGG - chr7_2:18954755-18954774 None:intergenic 25.0%
! ATGAGTTAGGATTATAACAA+GGG - chr7_2:18954818-18954837 None:intergenic 25.0%
!!! TGTTGATTTTGATCAATTTC+AGG - chr7_2:18955545-18955564 None:intergenic 25.0%
!! TGATATCTTTAACTTCATTG+AGG + chr7_2:18955622-18955641 Msa1051580:CDS 25.0%
! TCATGACAATCACTATTATT+AGG - chr7_2:18955719-18955738 None:intergenic 25.0%
! TTATTATTAGCATCCCAATT+TGG - chr7_2:18955758-18955777 None:intergenic 25.0%
!! TTCAAATCTTTGGAAAATCA+TGG + chr7_2:18955983-18956002 Msa1051580:CDS 25.0%
!! TCAAATCTTTGGAAAATCAT+GGG + chr7_2:18955984-18956003 Msa1051580:CDS 25.0%
! ATAAGCCTTCATATTTATCT+GGG - chr7_2:18954454-18954473 None:intergenic 25.0%
! AATAAGCCTTCATATTTATC+TGG - chr7_2:18954455-18954474 None:intergenic 25.0%
! ATTATATGGAAAGTGAGATT+TGG - chr7_2:18954503-18954522 None:intergenic 25.0%
!!! TGAAAGAAATTGATGATGAT+AGG - chr7_2:18954548-18954567 None:intergenic 25.0%
! AATGAACTACTTACTAGTTA+AGG - chr7_2:18954605-18954624 None:intergenic 25.0%
! CACGGATATTAAATATTTAG+AGG - chr7_2:18954629-18954648 None:intergenic 25.0%
! GTATATAGCTATGTTCTTAT+GGG - chr7_2:18954687-18954706 None:intergenic 25.0%
! GATTGATTACTTAACTCAAA+TGG - chr7_2:18954755-18954774 None:intergenic 25.0%
! ATGAGTTAGGATTATAACAA+GGG - chr7_2:18954818-18954837 None:intergenic 25.0%
!!! TGTTGATTTTGATCAATTTC+AGG - chr7_2:18955545-18955564 None:intergenic 25.0%
!! TGATATCTTTAACTTCATTG+AGG + chr7_2:18955622-18955641 Msa1051580:CDS 25.0%
! TCATGACAATCACTATTATT+AGG - chr7_2:18955719-18955738 None:intergenic 25.0%
! TTATTATTAGCATCCCAATT+TGG - chr7_2:18955758-18955777 None:intergenic 25.0%
!! TTCAAATCTTTGGAAAATCA+TGG + chr7_2:18955983-18956002 Msa1051580:CDS 25.0%
!! TCAAATCTTTGGAAAATCAT+GGG + chr7_2:18955984-18956003 Msa1051580:CDS 25.0%
!! AATTGATGATGATAGGTGAA+GGG - chr7_2:18954541-18954560 None:intergenic 30.0%
!! AAATTGATGATGATAGGTGA+AGG - chr7_2:18954542-18954561 None:intergenic 30.0%
! CCTAAAAACACCTTTTTACA+CGG - chr7_2:18954647-18954666 None:intergenic 30.0%
GGTATATAGCTATGTTCTTA+TGG - chr7_2:18954688-18954707 None:intergenic 30.0%
CACAACAAAAAAAGAACAAG+AGG - chr7_2:18954709-18954728 None:intergenic 30.0%
TTGTTGTGAAACAAGAAACT+AGG + chr7_2:18954721-18954740 Msa1051580:five_prime_UTR 30.0%
TGTTGTGAAACAAGAAACTA+GGG + chr7_2:18954722-18954741 Msa1051580:five_prime_UTR 30.0%
TTAGGATTATAACAAGGGTA+AGG - chr7_2:18954813-18954832 None:intergenic 30.0%
GATGAGTTAGGATTATAACA+AGG - chr7_2:18954819-18954838 None:intergenic 30.0%
!!! TAGAAGAAGCATTTTGATCA+GGG - chr7_2:18954881-18954900 None:intergenic 30.0%
!!! TTAGAAGAAGCATTTTGATC+AGG - chr7_2:18954882-18954901 None:intergenic 30.0%
GTTAGTGTATCAGGATTAAT+TGG - chr7_2:18955008-18955027 None:intergenic 30.0%
ATCCTGATACACTAACAAAT+TGG + chr7_2:18955012-18955031 Msa1051580:CDS 30.0%
TCCTGATACACTAACAAATT+GGG + chr7_2:18955013-18955032 Msa1051580:CDS 30.0%
ATGTTGAAACAAGATCTAAG+TGG + chr7_2:18955056-18955075 Msa1051580:CDS 30.0%
! AAATTTGCTCACAAAGAAAC+CGG + chr7_2:18955100-18955119 Msa1051580:CDS 30.0%
! TTTGAAGTTTCATCCTCATT+AGG - chr7_2:18955587-18955606 None:intergenic 30.0%
TCCAATGTTATCACCAAATT+GGG + chr7_2:18955742-18955761 Msa1051580:CDS 30.0%
TCTTGATATTGCAGAAAAGT+TGG - chr7_2:18955807-18955826 None:intergenic 30.0%
!!! TTTTTAGGGCTTCAAATCTT+TGG + chr7_2:18955973-18955992 Msa1051580:CDS 30.0%
!! AATTGATGATGATAGGTGAA+GGG - chr7_2:18954541-18954560 None:intergenic 30.0%
!! AAATTGATGATGATAGGTGA+AGG - chr7_2:18954542-18954561 None:intergenic 30.0%
! CCTAAAAACACCTTTTTACA+CGG - chr7_2:18954647-18954666 None:intergenic 30.0%
GGTATATAGCTATGTTCTTA+TGG - chr7_2:18954688-18954707 None:intergenic 30.0%
CACAACAAAAAAAGAACAAG+AGG - chr7_2:18954709-18954728 None:intergenic 30.0%
TTGTTGTGAAACAAGAAACT+AGG + chr7_2:18954721-18954740 Msa1051580:five_prime_UTR 30.0%
TGTTGTGAAACAAGAAACTA+GGG + chr7_2:18954722-18954741 Msa1051580:five_prime_UTR 30.0%
TTAGGATTATAACAAGGGTA+AGG - chr7_2:18954813-18954832 None:intergenic 30.0%
GATGAGTTAGGATTATAACA+AGG - chr7_2:18954819-18954838 None:intergenic 30.0%
!!! TAGAAGAAGCATTTTGATCA+GGG - chr7_2:18954881-18954900 None:intergenic 30.0%
!!! TTAGAAGAAGCATTTTGATC+AGG - chr7_2:18954882-18954901 None:intergenic 30.0%
GTTAGTGTATCAGGATTAAT+TGG - chr7_2:18955008-18955027 None:intergenic 30.0%
ATCCTGATACACTAACAAAT+TGG + chr7_2:18955012-18955031 Msa1051580:CDS 30.0%
TCCTGATACACTAACAAATT+GGG + chr7_2:18955013-18955032 Msa1051580:CDS 30.0%
ATGTTGAAACAAGATCTAAG+TGG + chr7_2:18955056-18955075 Msa1051580:CDS 30.0%
! AAATTTGCTCACAAAGAAAC+CGG + chr7_2:18955100-18955119 Msa1051580:CDS 30.0%
! TTTGAAGTTTCATCCTCATT+AGG - chr7_2:18955587-18955606 None:intergenic 30.0%
TCCAATGTTATCACCAAATT+GGG + chr7_2:18955742-18955761 Msa1051580:CDS 30.0%
TCTTGATATTGCAGAAAAGT+TGG - chr7_2:18955807-18955826 None:intergenic 30.0%
!!! TTTTTAGGGCTTCAAATCTT+TGG + chr7_2:18955973-18955992 Msa1051580:CDS 30.0%
TCATATTTATCTGGGTGTGA+TGG - chr7_2:18954446-18954465 None:intergenic 35.0%
TCACACCCAGATAAATATGA+AGG + chr7_2:18954446-18954465 Msa1051580:five_prime_UTR 35.0%
! TAGGTGAAGGGATTTCATTT+TGG - chr7_2:18954529-18954548 None:intergenic 35.0%
!!! AACCACTTTTTTTCTCTCCT+AGG + chr7_2:18954568-18954587 Msa1051580:five_prime_UTR 35.0%
AACCTAGGAGAGAAAAAAAG+TGG - chr7_2:18954573-18954592 None:intergenic 35.0%
TTAAGGACTATAGTGAACCT+AGG - chr7_2:18954588-18954607 None:intergenic 35.0%
!!! CCGTGTAAAAAGGTGTTTTT+AGG + chr7_2:18954644-18954663 Msa1051580:five_prime_UTR 35.0%
! CCTAAAGAGCAATAAGTTGA+AGG - chr7_2:18954789-18954808 None:intergenic 35.0%
CCTTCAACTTATTGCTCTTT+AGG + chr7_2:18954786-18954805 Msa1051580:CDS 35.0%
TGTGATGAAGAAGATGAGTT+AGG - chr7_2:18954831-18954850 None:intergenic 35.0%
!!! AGAAGAAGCATTTTGATCAG+GGG - chr7_2:18954880-18954899 None:intergenic 35.0%
TGATGAGTTGTTTGTGTGTA+GGG - chr7_2:18954978-18954997 None:intergenic 35.0%
ATGATGAGTTGTTTGTGTGT+AGG - chr7_2:18954979-18954998 None:intergenic 35.0%
TTCGATCTTCAAGACATGTT+AGG + chr7_2:18955224-18955243 Msa1051580:CDS 35.0%
TCGATCTTCAAGACATGTTA+GGG + chr7_2:18955225-18955244 Msa1051580:CDS 35.0%
AGCAAGAACAACATAGTTTC+TGG + chr7_2:18955320-18955339 Msa1051580:CDS 35.0%
ATCTTCTTCATCTCCTTCAT+CGG - chr7_2:18955387-18955406 None:intergenic 35.0%
CATTAGGCATCAATTGATGA+AGG - chr7_2:18955571-18955590 None:intergenic 35.0%
TCATCAATTGATGCCTAATG+AGG + chr7_2:18955571-18955590 Msa1051580:CDS 35.0%
CTCCAATGTTATCACCAAAT+TGG + chr7_2:18955741-18955760 Msa1051580:CDS 35.0%
! TCCCAATTTGGTGATAACAT+TGG - chr7_2:18955746-18955765 None:intergenic 35.0%
AATAACAATGATGCTGTCAC+TGG + chr7_2:18955776-18955795 Msa1051580:CDS 35.0%
TCAAGAACGAATCTATCATC+AGG + chr7_2:18955821-18955840 Msa1051580:CDS 35.0%
! AATCTTTGGAAAATCATGGG+AGG + chr7_2:18955987-18956006 Msa1051580:CDS 35.0%
TCATATTTATCTGGGTGTGA+TGG - chr7_2:18954446-18954465 None:intergenic 35.0%
TCACACCCAGATAAATATGA+AGG + chr7_2:18954446-18954465 Msa1051580:five_prime_UTR 35.0%
! TAGGTGAAGGGATTTCATTT+TGG - chr7_2:18954529-18954548 None:intergenic 35.0%
!!! AACCACTTTTTTTCTCTCCT+AGG + chr7_2:18954568-18954587 Msa1051580:five_prime_UTR 35.0%
AACCTAGGAGAGAAAAAAAG+TGG - chr7_2:18954573-18954592 None:intergenic 35.0%
TTAAGGACTATAGTGAACCT+AGG - chr7_2:18954588-18954607 None:intergenic 35.0%
!!! CCGTGTAAAAAGGTGTTTTT+AGG + chr7_2:18954644-18954663 Msa1051580:five_prime_UTR 35.0%
! CCTAAAGAGCAATAAGTTGA+AGG - chr7_2:18954789-18954808 None:intergenic 35.0%
CCTTCAACTTATTGCTCTTT+AGG + chr7_2:18954786-18954805 Msa1051580:CDS 35.0%
TGTGATGAAGAAGATGAGTT+AGG - chr7_2:18954831-18954850 None:intergenic 35.0%
!!! AGAAGAAGCATTTTGATCAG+GGG - chr7_2:18954880-18954899 None:intergenic 35.0%
TGATGAGTTGTTTGTGTGTA+GGG - chr7_2:18954978-18954997 None:intergenic 35.0%
ATGATGAGTTGTTTGTGTGT+AGG - chr7_2:18954979-18954998 None:intergenic 35.0%
TTCGATCTTCAAGACATGTT+AGG + chr7_2:18955224-18955243 Msa1051580:CDS 35.0%
TCGATCTTCAAGACATGTTA+GGG + chr7_2:18955225-18955244 Msa1051580:CDS 35.0%
AGCAAGAACAACATAGTTTC+TGG + chr7_2:18955320-18955339 Msa1051580:CDS 35.0%
ATCTTCTTCATCTCCTTCAT+CGG - chr7_2:18955387-18955406 None:intergenic 35.0%
CATTAGGCATCAATTGATGA+AGG - chr7_2:18955571-18955590 None:intergenic 35.0%
TCATCAATTGATGCCTAATG+AGG + chr7_2:18955571-18955590 Msa1051580:CDS 35.0%
CTCCAATGTTATCACCAAAT+TGG + chr7_2:18955741-18955760 Msa1051580:CDS 35.0%
! TCCCAATTTGGTGATAACAT+TGG - chr7_2:18955746-18955765 None:intergenic 35.0%
AATAACAATGATGCTGTCAC+TGG + chr7_2:18955776-18955795 Msa1051580:CDS 35.0%
TCAAGAACGAATCTATCATC+AGG + chr7_2:18955821-18955840 Msa1051580:CDS 35.0%
! AATCTTTGGAAAATCATGGG+AGG + chr7_2:18955987-18956006 Msa1051580:CDS 35.0%
GGGTAAGGAAAGTAAAAGGA+GGG - chr7_2:18954860-18954879 None:intergenic 40.0%
TCAGGGGTAAGGAAAGTAAA+AGG - chr7_2:18954864-18954883 None:intergenic 40.0%
!! AAGCATTTTGATCAGGGGTA+AGG - chr7_2:18954875-18954894 None:intergenic 40.0%
!! GCCCAATTTGTTAGTGTATC+AGG - chr7_2:18955017-18955036 None:intergenic 40.0%
AAAGAAACCGGTGAAGAAAC+CGG + chr7_2:18955112-18955131 Msa1051580:CDS 40.0%
AGCAAGATTCACACATCTCA+AGG + chr7_2:18955152-18955171 Msa1051580:CDS 40.0%
GCAAGATTCACACATCTCAA+GGG + chr7_2:18955153-18955172 Msa1051580:CDS 40.0%
GAAAGAATCTTCGAAGACGA+AGG + chr7_2:18955439-18955458 Msa1051580:CDS 40.0%
TTCGAAGACGAAGGATTCTA+GGG + chr7_2:18955448-18955467 Msa1051580:CDS 40.0%
AGAAAGCAAGAGCAAGAACA+AGG + chr7_2:18955471-18955490 Msa1051580:CDS 40.0%
GAAAGCAAGAGCAAGAACAA+GGG + chr7_2:18955472-18955491 Msa1051580:CDS 40.0%
GGGTAAGGAAAGTAAAAGGA+GGG - chr7_2:18954860-18954879 None:intergenic 40.0%
TCAGGGGTAAGGAAAGTAAA+AGG - chr7_2:18954864-18954883 None:intergenic 40.0%
!! AAGCATTTTGATCAGGGGTA+AGG - chr7_2:18954875-18954894 None:intergenic 40.0%
!! GCCCAATTTGTTAGTGTATC+AGG - chr7_2:18955017-18955036 None:intergenic 40.0%
AAAGAAACCGGTGAAGAAAC+CGG + chr7_2:18955112-18955131 Msa1051580:CDS 40.0%
AGCAAGATTCACACATCTCA+AGG + chr7_2:18955152-18955171 Msa1051580:CDS 40.0%
GCAAGATTCACACATCTCAA+GGG + chr7_2:18955153-18955172 Msa1051580:CDS 40.0%
GAAAGAATCTTCGAAGACGA+AGG + chr7_2:18955439-18955458 Msa1051580:CDS 40.0%
TTCGAAGACGAAGGATTCTA+GGG + chr7_2:18955448-18955467 Msa1051580:CDS 40.0%
AGAAAGCAAGAGCAAGAACA+AGG + chr7_2:18955471-18955490 Msa1051580:CDS 40.0%
GAAAGCAAGAGCAAGAACAA+GGG + chr7_2:18955472-18955491 Msa1051580:CDS 40.0%
!! TTATCTGGGTGTGATGGACA+AGG - chr7_2:18954440-18954459 None:intergenic 45.0%
GGGGTAAGGAAAGTAAAAGG+AGG - chr7_2:18954861-18954880 None:intergenic 45.0%
TAGGGAACAGAAATTGCTGC+TGG - chr7_2:18954960-18954979 None:intergenic 45.0%
! TTCTTAGCCGGTTTCTTCAC+CGG - chr7_2:18955122-18955141 None:intergenic 45.0%
AGAAACCGGCTAAGAAAGAC+AGG + chr7_2:18955126-18955145 Msa1051580:CDS 45.0%
CTATGCCTGTCTTTCTTAGC+CGG - chr7_2:18955134-18955153 None:intergenic 45.0%
TCAAGGGTTGAGAGACAGAA+GGG + chr7_2:18955169-18955188 Msa1051580:CDS 45.0%
AAGCCAGCAACACACTTGAA+TGG + chr7_2:18955255-18955274 Msa1051580:CDS 45.0%
CTTCGAAGACGAAGGATTCT+AGG + chr7_2:18955447-18955466 Msa1051580:CDS 45.0%
!! TTATCTGGGTGTGATGGACA+AGG - chr7_2:18954440-18954459 None:intergenic 45.0%
GGGGTAAGGAAAGTAAAAGG+AGG - chr7_2:18954861-18954880 None:intergenic 45.0%
TAGGGAACAGAAATTGCTGC+TGG - chr7_2:18954960-18954979 None:intergenic 45.0%
! TTCTTAGCCGGTTTCTTCAC+CGG - chr7_2:18955122-18955141 None:intergenic 45.0%
AGAAACCGGCTAAGAAAGAC+AGG + chr7_2:18955126-18955145 Msa1051580:CDS 45.0%
CTATGCCTGTCTTTCTTAGC+CGG - chr7_2:18955134-18955153 None:intergenic 45.0%
TCAAGGGTTGAGAGACAGAA+GGG + chr7_2:18955169-18955188 Msa1051580:CDS 45.0%
AAGCCAGCAACACACTTGAA+TGG + chr7_2:18955255-18955274 Msa1051580:CDS 45.0%
CTTCGAAGACGAAGGATTCT+AGG + chr7_2:18955447-18955466 Msa1051580:CDS 45.0%
!! GTGTGATGGACAAGGCTCAT+GGG - chr7_2:18954432-18954451 None:intergenic 50.0%
CTCAAGGGTTGAGAGACAGA+AGG + chr7_2:18955168-18955187 Msa1051580:CDS 50.0%
GAGCCATTCAAGTGTGTTGC+TGG - chr7_2:18955261-18955280 None:intergenic 50.0%
TCTTCTTCCTCGTCCGATGA+AGG + chr7_2:18955371-18955390 Msa1051580:CDS 50.0%
TTCATCTCCTTCATCGGACG+AGG - chr7_2:18955381-18955400 None:intergenic 50.0%
!! GTGTGATGGACAAGGCTCAT+GGG - chr7_2:18954432-18954451 None:intergenic 50.0%
CTCAAGGGTTGAGAGACAGA+AGG + chr7_2:18955168-18955187 Msa1051580:CDS 50.0%
GAGCCATTCAAGTGTGTTGC+TGG - chr7_2:18955261-18955280 None:intergenic 50.0%
TCTTCTTCCTCGTCCGATGA+AGG + chr7_2:18955371-18955390 Msa1051580:CDS 50.0%
TTCATCTCCTTCATCGGACG+AGG - chr7_2:18955381-18955400 None:intergenic 50.0%
GATGGACAAGGCTCATGGGT+TGG - chr7_2:18954428-18954447 None:intergenic 55.0%
!! GGTGTGATGGACAAGGCTCA+TGG - chr7_2:18954433-18954452 None:intergenic 55.0%
GACTTTCCATCGAGATCGCG+AGG + chr7_2:18955195-18955214 Msa1051580:CDS 55.0%
GAACTTCCTCGCGATCTCGA+TGG - chr7_2:18955204-18955223 None:intergenic 55.0%
!! GTTTCTGGCGATGACGACGA+TGG + chr7_2:18955335-18955354 Msa1051580:CDS 55.0%
GATGGACAAGGCTCATGGGT+TGG - chr7_2:18954428-18954447 None:intergenic 55.0%
!! GGTGTGATGGACAAGGCTCA+TGG - chr7_2:18954433-18954452 None:intergenic 55.0%
GACTTTCCATCGAGATCGCG+AGG + chr7_2:18955195-18955214 Msa1051580:CDS 55.0%
GAACTTCCTCGCGATCTCGA+TGG - chr7_2:18955204-18955223 None:intergenic 55.0%
!! GTTTCTGGCGATGACGACGA+TGG + chr7_2:18955335-18955354 Msa1051580:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 18954424 18956038 18954424 ID=Msa1051580;Name=Msa1051580
chr7_2 mRNA 18954424 18956038 18954424 ID=Msa1051580-mRNA-1;Parent=Msa1051580;Name=Msa1051580-mRNA-1;_AED=0.09;_eAED=0.09;_QI=356|0|0.5|1|1|1|2|0|378
chr7_2 exon 18954424 18955842 18954424 ID=Msa1051580-mRNA-1:exon:4168;Parent=Msa1051580-mRNA-1
chr7_2 exon 18955965 18956038 18955965 ID=Msa1051580-mRNA-1:exon:4169;Parent=Msa1051580-mRNA-1
chr7_2 five_prime_UTR 18954424 18954779 18954424 ID=Msa1051580-mRNA-1:five_prime_utr;Parent=Msa1051580-mRNA-1
chr7_2 CDS 18954780 18955842 18954780 ID=Msa1051580-mRNA-1:cds;Parent=Msa1051580-mRNA-1
chr7_2 CDS 18955965 18956038 18955965 ID=Msa1051580-mRNA-1:cds;Parent=Msa1051580-mRNA-1
Gene Sequence

>Msa1051580

ATGTTTCCTTCAACTTATTGCTCTTTAGGTCCTTACCCTTGTTATAATCCTAACTCATCTTCTTCATCACATTCATACCCTCCTTTTACTTTCCTTACCCCTGATCAAAATGCTTCTTCTAACAACAACAACAATAACAACAACAGCAACAACAACATCAACAACTTTCTTCATGATCCAGCAGCAATTTCTGTTCCCTACACACAAACAACTCATCATCATGTTCCAATTAATCCTGATACACTAACAAATTGGGCTGTTGCTGACTATGCAGCTATGTTGAAACAAGATCTAAGTGGTTCTTCTCATTACAATCTCTCAAATTTGCTCACAAAGAAACCGGTGAAGAAACCGGCTAAGAAAGACAGGCATAGCAAGATTCACACATCTCAAGGGTTGAGAGACAGAAGGGTGAGACTTTCCATCGAGATCGCGAGGAAGTTCTTCGATCTTCAAGACATGTTAGGGTTTGACAAAGCCAGCAACACACTTGAATGGCTCTTCAATAAATCAAAAGAAGCAATTGAAGAGTTAACTAGAAGCAAGAACAACATAGTTTCTGGCGATGACGACGATGGTGATCATAGCTTCTCTTCTTCCTCGTCCGATGAAGGAGATGAAGAAGATTATACTAAAAATAGAAAGATGAAAAGGGCACAGAAAGAATCTTCGAAGACGAAGGATTCTAGGGAGAAAGCAAGAGCAAGAACAAGGGAAAGAGCTAGTGAGATGAAGATGCAAGAAGATTTGAAAGAAAAATATCCTGAAATTGATCAAAATCAACAAATCCTTCATCAATTGATGCCTAATGAGGATGAAACTTCAAAATCACTTCAAAGAGATGATATCTTTAACTTCATTGAGGAATCTATTGTTATTAAGAGAAAGTTGAAGAAATCTTCTTCTCATCATTATCAACAAAACACTAATTTGATGCCTAATAATAGTGATTGTCATGACTCTCCAATGTTATCACCAAATTGGGATGCTAATAATAATAACAATGATGCTGTCACTGGAAGATCCAACTTTTCTGCAATATCAAGAACGAATCTATCATCAGGTTTAAACTTTTTAGGGCTTCAAATCTTTGGAAAATCATGGGAGGATTGCAATACCAGTCCAAATCGATATTAG
Protein sequence

>Msa1051580

MFPSTYCSLGPYPCYNPNSSSSSHSYPPFTFLTPDQNASSNNNNNNNNSNNNINNFLHDPAAISVPYTQTTHHHVPINPDTLTNWAVADYAAMLKQDLSGSSHYNLSNLLTKKPVKKPAKKDRHSKIHTSQGLRDRRVRLSIEIARKFFDLQDMLGFDKASNTLEWLFNKSKEAIEELTRSKNNIVSGDDDDGDHSFSSSSSDEGDEEDYTKNRKMKRAQKESSKTKDSREKARARTRERASEMKMQEDLKEKYPEIDQNQQILHQLMPNEDETSKSLQRDDIFNFIEESIVIKRKLKKSSSHHYQQNTNLMPNNSDCHDSPMLSPNWDANNNNNDAVTGRSNFSAISRTNLSSGLNFLGLQIFGKSWEDCNTSPNRY*