AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1054230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1054230 MtrunA17_Chr7g0224271 98.665 674 9 0 1 674 1 674 0.0 1385
Msa1054230 MtrunA17_Chr4g0057891 41.604 399 211 6 288 674 133 521 1.45e-96 307
Msa1054230 MtrunA17_Chr8g0376381 41.895 401 191 10 284 673 153 522 1.68e-94 302
Msa1054230 MtrunA17_Chr2g0306641 42.250 400 190 10 290 674 115 488 2.83e-93 298
Msa1054230 MtrunA17_Chr3g0110971 35.878 524 268 13 184 673 98 587 4.17e-93 300
Msa1054230 MtrunA17_Chr7g0245601 41.910 377 186 6 304 673 442 792 2.23e-92 304
Msa1054230 MtrunA17_Chr5g0447691 40.204 393 205 5 293 673 170 544 3.86e-88 285
Msa1054230 MtrunA17_Chr8g0357601 40.863 394 206 6 292 673 174 552 4.16e-88 286
Msa1054230 MtrunA17_Chr4g0057291 38.303 389 217 7 294 672 286 661 5.97e-82 273
Msa1054230 MtrunA17_Chr1g0159461 39.589 389 201 6 299 673 224 592 7.07e-81 268
Msa1054230 MtrunA17_Chr2g0319091 36.490 433 239 5 258 672 164 578 5.71e-79 262
Msa1054230 MtrunA17_Chr4g0038761 38.710 372 209 6 304 673 88 442 2.86e-78 256
Msa1054230 MtrunA17_Chr3g0101141 32.734 556 296 10 162 673 21 542 3.49e-78 259
Msa1054230 MtrunA17_Chr5g0445331 33.406 461 267 7 233 673 92 532 8.24e-78 258
Msa1054230 MtrunA17_Chr3g0125811 37.887 388 209 5 299 673 202 570 3.05e-76 255
Msa1054230 MtrunA17_Chr4g0076091 35.878 393 221 5 295 674 170 544 3.64e-71 240
Msa1054230 MtrunA17_Chr2g0290241 33.666 401 219 8 295 674 52 426 4.02e-69 231
Msa1054230 MtrunA17_Chr4g0031721 34.000 400 234 6 286 672 301 683 3.20e-65 228
Msa1054230 MtrunA17_Chr1g0183641 35.128 390 224 10 290 674 113 478 7.66e-65 222
Msa1054230 MtrunA17_Chr5g0424131 36.930 417 210 17 288 674 107 500 1.56e-64 222
Msa1054230 MtrunA17_Chr1g0200401 34.140 413 220 14 290 673 70 459 7.60e-64 218
Msa1054230 MtrunA17_Chr3g0114841 37.101 407 219 14 290 674 110 501 1.64e-63 219
Msa1054230 MtrunA17_Chr3g0085681 30.140 428 227 5 301 673 26 436 8.83e-62 212
Msa1054230 MtrunA17_Chr7g0238891 32.398 392 234 5 295 673 367 740 4.74e-59 212
Msa1054230 MtrunA17_Chr4g0031701 31.910 398 234 5 291 670 245 623 8.72e-59 209
Msa1054230 MtrunA17_Chr4g0031731 31.910 398 234 5 291 670 245 623 1.24e-57 206
Msa1054230 MtrunA17_Chr2g0328391 31.807 393 237 6 294 672 293 668 1.12e-56 204
Msa1054230 MtrunA17_Chr4g0031741 31.714 391 234 7 295 672 279 649 1.50e-56 203
Msa1054230 MtrunA17_Chr2g0328341 30.848 389 240 6 295 672 267 637 2.03e-56 202
Msa1054230 MtrunA17_Chr4g0031711 31.671 401 237 7 290 672 351 732 2.44e-55 201
Msa1054230 MtrunA17_Chr2g0328381 30.334 389 242 6 295 672 270 640 7.91e-55 198
Msa1054230 MtrunA17_Chr2g0328411 29.771 393 242 5 293 672 282 653 3.91e-54 197
Msa1054230 MtrunA17_Chr2g0328431 30.227 397 235 8 293 672 282 653 2.04e-51 189
Msa1054230 MtrunA17_Chr4g0039661 33.962 371 208 8 316 673 209 555 1.70e-50 184
Msa1054230 MtrunA17_Chr2g0294461 31.683 404 246 10 286 673 196 585 2.99e-49 181
Msa1054230 MtrunA17_Chr3g0085361 30.747 348 181 5 381 673 8 350 2.56e-48 173
Msa1054230 MtrunA17_Chr5g0401131 30.923 401 240 11 293 673 107 490 7.38e-48 176
Msa1054230 MtrunA17_Chr2g0294471 29.703 404 251 11 286 673 190 576 1.86e-46 173
Msa1054230 MtrunA17_Chr2g0328401 27.933 358 226 5 295 640 285 622 5.85e-45 170
Msa1054230 MtrunA17_Chr2g0328361 28.093 388 227 7 295 672 269 614 1.59e-44 169
Msa1054230 MtrunA17_Chr7g0270031 29.723 397 253 9 286 673 175 554 3.41e-44 166
Msa1054230 MtrunA17_Chr8g0383981 32.426 404 221 15 287 672 126 495 5.34e-42 159
Msa1054230 MtrunA17_Chr2g0328371 34.286 245 150 4 387 620 1 245 3.34e-41 150
Msa1054230 MtrunA17_Chr1g0194621 28.571 406 265 11 283 673 123 518 4.69e-41 157
Msa1054230 MtrunA17_Chr5g0396491 36.797 231 124 2 289 516 45 256 7.08e-40 153
Msa1054230 MtrunA17_Chr5g0396491 43.333 150 85 0 525 674 330 479 5.62e-39 150
Msa1054230 MtrunA17_Chr4g0038851 44.595 148 82 0 526 673 322 469 1.84e-39 151
Msa1054230 MtrunA17_Chr4g0038851 35.088 228 132 2 289 516 37 248 9.98e-35 137
Msa1054230 MtrunA17_Chr7g0236851 34.007 297 175 8 392 672 365 656 1.67e-38 151
Msa1054230 MtrunA17_Chr7g0230771 29.124 388 238 11 297 674 372 732 8.90e-36 144
Msa1054230 MtrunA17_Chr7g0230731 29.124 388 238 11 297 674 369 729 4.41e-35 142
Msa1054230 MtrunA17_Chr6g0468561 29.383 405 216 13 301 673 296 662 4.21e-34 138
Msa1054230 MtrunA17_Chr2g0329591 26.329 395 253 10 300 673 74 451 7.84e-33 132
Msa1054230 MtrunA17_Chr4g0053901 25.714 420 254 9 300 672 59 467 1.76e-32 131
Msa1054230 MtrunA17_Chr4g0012071 28.238 386 235 12 301 674 197 552 5.32e-32 131
Msa1054230 MtrunA17_Chr5g0401421 25.592 422 254 12 300 672 35 445 3.53e-31 127
Msa1054230 MtrunA17_Chr8g0373561 27.226 393 242 14 297 674 179 542 8.13e-29 121
Msa1054230 MtrunA17_Chr8g0344101 23.587 407 251 12 299 673 222 600 2.02e-19 92.8
Msa1054230 MtrunA17_Chr2g0323111 26.286 175 120 3 505 672 133 305 4.98e-14 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1054230 AT1G66350.1 44.103 390 184 11 291 674 146 507 1.08e-97 310
Msa1054230 AT3G03450.1 42.092 392 198 8 291 673 174 545 3.88e-95 304
Msa1054230 AT3G54220.1 41.709 398 196 6 285 672 278 649 1.23e-92 301
Msa1054230 AT1G14920.1 40.712 393 197 9 291 673 163 529 3.14e-90 291
Msa1054230 AT5G17490.1 42.967 391 190 10 291 673 151 516 3.02e-89 288
Msa1054230 AT2G01570.1 40.458 393 198 10 291 673 215 581 4.11e-87 285
Msa1054230 AT5G41920.1 40.759 395 202 9 287 673 30 400 1.90e-86 277
Msa1054230 AT2G04890.1 39.949 393 209 5 287 673 42 413 5.42e-84 271
Msa1054230 AT5G48150.1 39.481 385 204 5 301 673 123 490 5.57e-83 271
Msa1054230 AT5G48150.4 39.481 385 204 5 301 673 123 490 5.57e-83 271
Msa1054230 AT5G48150.2 39.481 385 204 5 301 673 123 490 5.57e-83 271
Msa1054230 AT5G48150.3 39.481 385 204 5 301 673 123 490 5.57e-83 271
Msa1054230 AT1G50600.3 38.560 389 209 6 297 673 156 526 1.89e-73 246
Msa1054230 AT1G50600.1 39.075 389 207 7 297 673 227 597 4.76e-73 247
Msa1054230 AT1G50600.2 38.560 389 209 6 297 673 231 601 7.46e-73 247
Msa1054230 AT1G21450.1 36.434 387 217 4 299 673 224 593 2.30e-72 245
Msa1054230 AT1G55580.1 37.438 406 226 8 296 673 40 445 1.35e-71 239
Msa1054230 AT4G17230.1 36.061 391 220 4 295 673 153 525 5.49e-68 232
Msa1054230 AT4G17230.2 36.061 391 220 4 295 673 153 525 5.49e-68 232
Msa1054230 AT1G63100.1 34.596 396 227 7 295 672 247 628 4.28e-67 232
Msa1054230 AT1G63100.2 34.596 396 227 7 295 672 272 653 7.86e-67 232
Msa1054230 AT1G07520.2 31.527 406 248 8 283 672 137 528 6.41e-59 207
Msa1054230 AT1G07520.4 31.527 406 248 8 283 672 137 528 6.41e-59 207
Msa1054230 AT1G07520.3 31.592 402 247 7 286 672 305 693 2.05e-58 209
Msa1054230 AT1G07520.1 31.592 402 247 7 286 672 304 692 2.32e-58 209
Msa1054230 AT3G46600.1 30.326 399 246 5 288 673 200 579 3.68e-57 204
Msa1054230 AT5G59450.1 29.442 394 246 6 295 673 222 598 4.77e-57 204
Msa1054230 AT3G46600.3 30.326 399 246 5 288 673 168 547 8.73e-57 202
Msa1054230 AT3G46600.2 30.670 388 237 5 299 673 3 371 3.47e-56 196
Msa1054230 AT1G07530.1 31.538 390 235 6 297 672 393 764 3.44e-55 202
Msa1054230 AT2G29065.1 29.820 389 244 7 300 672 252 627 1.07e-54 198
Msa1054230 AT5G66770.1 35.038 391 217 10 297 673 217 584 1.87e-53 194
Msa1054230 AT2G29060.2 30.612 392 238 7 297 672 281 654 8.92e-53 193
Msa1054230 AT2G29060.1 30.612 392 238 7 297 672 318 691 1.25e-52 193
Msa1054230 AT2G37650.2 30.827 399 229 7 295 673 330 701 1.63e-52 193
Msa1054230 AT2G37650.1 31.078 399 228 8 295 673 342 713 2.26e-52 193
Msa1054230 AT4G08250.1 32.432 407 228 14 288 672 95 476 3.85e-49 179
Msa1054230 AT3G50650.1 33.962 371 205 9 320 673 195 542 7.34e-46 171
Msa1054230 AT4G37650.1 29.397 398 243 10 300 673 146 529 2.87e-43 164
Msa1054230 AT5G52510.1 30.250 400 224 9 301 673 269 640 6.94e-40 155
Msa1054230 AT1G50420.1 45.333 150 82 0 525 674 331 480 6.44e-39 150
Msa1054230 AT1G50420.1 38.496 226 125 2 291 516 48 259 5.59e-38 148
Msa1054230 AT3G49950.1 28.678 401 237 10 299 672 29 407 5.29e-33 132
Msa1054230 AT2G45160.1 27.829 327 204 9 367 674 327 640 2.18e-28 121
Msa1054230 AT4G00150.1 27.431 401 238 13 282 674 200 555 2.64e-28 120
Msa1054230 AT4G36710.1 26.884 398 256 11 287 673 112 485 5.60e-28 118
Msa1054230 AT3G60630.1 28.852 305 191 10 388 674 327 623 9.65e-28 119
Msa1054230 AT3G13840.1 24.557 395 245 14 300 673 148 510 2.25e-14 76.6

Find 162 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCAATATCAACAATTT+TGG 0.134391 7_2:-25604670 Msa1054230:CDS
TGTTGCATATATTGATTATT+TGG 0.203768 7_2:+25604801 None:intergenic
GTAGAAGCTTGTGGTGAATT+AGG 0.209436 7_2:+25604981 None:intergenic
TGTATTCATGTGATGGTTAT+AGG 0.213345 7_2:-25602894 Msa1054230:CDS
CTATCCAAAATTGTTGATAT+TGG 0.238972 7_2:+25604666 None:intergenic
AATAGTAATAATCAACAAAA+AGG 0.242712 7_2:-25604861 Msa1054230:CDS
TGCTTCAAACGCCTCGAAAA+TGG 0.246534 7_2:+25603587 None:intergenic
GGCAAGCTTGGTAGACAATT+TGG 0.255349 7_2:+25603646 None:intergenic
GACGCAATCAAGGATATTAT+TGG 0.274678 7_2:-25602920 Msa1054230:CDS
GGCATGAGAGGTTGGAGAAA+TGG 0.280692 7_2:-25602999 Msa1054230:CDS
GCCAGCTATTGAAGAGTTCT+TGG 0.285111 7_2:-25604760 Msa1054230:CDS
TTAGGGTTAATGTTTCTAAT+TGG 0.288688 7_2:+25604999 None:intergenic
GATGGTAAGTTTAGGCTTTG+TGG 0.292426 7_2:+25605128 None:intergenic
CTTGATTGCGTCACTGCATT+AGG 0.296738 7_2:+25602931 None:intergenic
TAGAAGCTTGTGGTGAATTA+GGG 0.297220 7_2:+25604982 None:intergenic
TAACCGTCTCCACCGTGTTC+CGG 0.313436 7_2:-25603283 Msa1054230:CDS
GTATCATCAAATGGGATCTA+TGG 0.314373 7_2:-25604538 Msa1054230:CDS
TCATGAAATCATTATCTAAA+TGG 0.317694 7_2:+25605030 None:intergenic
TCTCTCAAATTTGACTTAGA+TGG 0.323118 7_2:-25605107 Msa1054230:CDS
TTGATGATGACTTGGTTGTT+AGG 0.342078 7_2:+25603763 None:intergenic
ATTTGTTTGATTTGAAGAAT+TGG 0.347254 7_2:+25605214 None:intergenic
CATCGACCTTGATATCCTTC+AGG 0.351187 7_2:-25603547 Msa1054230:CDS
AACCGTCTCCACCGTGTTCC+GGG 0.356238 7_2:-25603282 Msa1054230:CDS
TATAGGTTAACTGAGGATAA+AGG 0.379685 7_2:-25602877 Msa1054230:CDS
ACGCAATCAAGGATATTATT+GGG 0.380749 7_2:-25602919 Msa1054230:CDS
ATAAAGGTTGTTTGTTGCTT+GGG 0.381702 7_2:-25602861 Msa1054230:CDS
CTAACAGTTCCTATTCCAAT+TGG 0.386725 7_2:-25604435 Msa1054230:CDS
TGGTGCTTGGTCTCTTATCA+TGG 0.387315 7_2:+25603236 None:intergenic
GATAAAGGTTGTTTGTTGCT+TGG 0.388205 7_2:-25602862 Msa1054230:CDS
GATAGAGAGGCATGAGAGGT+TGG 0.388288 7_2:-25603007 Msa1054230:CDS
GCTTCCCACATTGAACTATC+TGG 0.392042 7_2:+25605065 None:intergenic
AGGACTTCCATAGGGTTTGA+TGG 0.398057 7_2:+25603678 None:intergenic
TCCTCAAGTTGTTCTCCAAC+GGG 0.400097 7_2:+25603363 None:intergenic
AATGCAAAGCCTCGAGAAAT+CGG 0.401833 7_2:+25603157 None:intergenic
GCCAATGCCTGCATGAATGC+AGG 0.402364 7_2:+25603513 None:intergenic
GTCACTGCATTAGGACTTAG+AGG 0.403869 7_2:+25602940 None:intergenic
ATGCAAATTCAAAGCCTATC+AGG 0.406320 7_2:-25604924 Msa1054230:CDS
ATTTGAGAGATGGTAAGTTT+AGG 0.417615 7_2:+25605120 None:intergenic
TGATGATGACTTGGTTGTTA+GGG 0.418899 7_2:+25603764 None:intergenic
TTTGTTTGATTTGAAGAATT+GGG 0.419908 7_2:+25605215 None:intergenic
CAGGCATTGGCTGCACGGCC+CGG 0.426882 7_2:-25603501 Msa1054230:CDS
GAGTGATATGTATCATCAAA+TGG 0.430639 7_2:-25604547 Msa1054230:CDS
TCCACAAGTGTCACTATGTT+TGG 0.431028 7_2:+25603216 None:intergenic
ATATATTTGCACCGGAGATT+CGG 0.433485 7_2:-25603059 Msa1054230:CDS
CTCGAAAATGGCTTGGTTAG+CGG 0.435294 7_2:+25603599 None:intergenic
GGCCGTGCATCGAATCAGTT+AGG 0.436845 7_2:-25603446 Msa1054230:CDS
TCATGCAGGCATTGGCTGCA+CGG 0.438295 7_2:-25603506 Msa1054230:CDS
CCGGAGGAAAGGAGCACCAC+CGG 0.441381 7_2:+25603482 None:intergenic
GGAGAAATGGAGGAAGATAA+TGG 0.442415 7_2:-25602986 Msa1054230:CDS
GGAAACCGGACGATGCTTAA+CGG 0.443248 7_2:-25603424 Msa1054230:CDS
AAAATGATGAATTCACTTTG+TGG 0.443783 7_2:-25605272 Msa1054230:CDS
ATTACCTGCTCAATTCCAAT+TGG 0.449064 7_2:+25604420 None:intergenic
GCTACGATGTTCCGAATCTC+CGG 0.453601 7_2:+25603048 None:intergenic
ATCGAATCAGTTAGGGAAAC+CGG 0.454197 7_2:-25603438 Msa1054230:CDS
CAAAATTCTTCAAGCTATTG+TGG 0.454394 7_2:-25604633 Msa1054230:CDS
TAAATCACTACAAGAATTTG+TGG 0.454463 7_2:+25605172 None:intergenic
TGCTCAATTCCAATTGGAAT+AGG 0.456239 7_2:+25604426 None:intergenic
GTGTTCCGGGCAATCATCTC+GGG 0.457485 7_2:-25603269 Msa1054230:CDS
GGTCTCTTATCATGGACAAC+AGG 0.459964 7_2:+25603244 None:intergenic
AGCAAGAAGCAAGCCATAAC+GGG 0.462121 7_2:-25603194 Msa1054230:CDS
GCCCTTGGTGCTGATTCCAC+TGG 0.463860 7_2:+25603096 None:intergenic
CCGTGTAGGCGAGGCTTTGG+CGG 0.466492 7_2:-25603316 Msa1054230:CDS
ACATTGAACTATCTGGTGAT+TGG 0.469373 7_2:+25605072 None:intergenic
TACTGCTCCACCTTAGCCCT+TGG 0.470876 7_2:+25603081 None:intergenic
TCCAAGAACTCTTCAATAGC+TGG 0.478205 7_2:+25604759 None:intergenic
AACACGGTGGAGACGGTTAA+CGG 0.479130 7_2:+25603287 None:intergenic
ATGAGAGGTTGGAGAAATGG+AGG 0.480103 7_2:-25602996 Msa1054230:CDS
CACTGGAAAAGTGGCGTCCA+AGG 0.485225 7_2:+25603113 None:intergenic
TATTCCAATTGGAATTGAGC+AGG 0.485481 7_2:-25604424 Msa1054230:intron
TTCCATAGGGTTTGATGGGA+AGG 0.488537 7_2:+25603683 None:intergenic
GCATTGGCTGCACGGCCCGG+TGG 0.490051 7_2:-25603498 Msa1054230:CDS
CGTGTTCCGGGCAATCATCT+CGG 0.491435 7_2:-25603270 Msa1054230:CDS
ATCGACCTTGATATCCTTCA+GGG 0.491571 7_2:-25603546 Msa1054230:CDS
GCCTGCATTCATGCAGGCAT+TGG 0.492416 7_2:-25603514 Msa1054230:CDS
GTGTCACTATGTTTGGTGCT+TGG 0.501545 7_2:+25603223 None:intergenic
CCGCCAAAGCCTCGCCTACA+CGG 0.501971 7_2:+25603316 None:intergenic
AGTGATATGTATCATCAAAT+GGG 0.505152 7_2:-25604546 Msa1054230:CDS
TACCTTCCCATCAAACCCTA+TGG 0.506323 7_2:-25603685 Msa1054230:CDS
CTAATGCAGTGACGCAATCA+AGG 0.506595 7_2:-25602930 Msa1054230:CDS
TCCCTAACTGATTCGATGCA+CGG 0.507816 7_2:+25603444 None:intergenic
AGAGGATAGAGAGGCATGAG+AGG 0.511494 7_2:-25603011 Msa1054230:CDS
CCGGTGGTGCTCCTTTCCTC+CGG 0.515736 7_2:-25603482 Msa1054230:CDS
CAAACGCCTCGAAAATGGCT+TGG 0.518445 7_2:+25603592 None:intergenic
CACAAGCTCTACAACAAGAA+AGG 0.518966 7_2:-25604500 Msa1054230:CDS
AAGGTGAGGAGAGGATAGAG+AGG 0.521365 7_2:-25603020 Msa1054230:CDS
GGAAGATAATGGAAGGGAAA+GGG 0.523029 7_2:-25602975 Msa1054230:CDS
TAACTCCGTTAAGCATCGTC+CGG 0.530160 7_2:+25603419 None:intergenic
TTCCTCCGGATCACCGGCGT+AGG 0.532300 7_2:-25603468 Msa1054230:CDS
CCAATGGCCTGCATTCATGC+AGG 0.532300 7_2:-25603520 Msa1054230:CDS
GCTCCTTTCCTCCGGATCAC+CGG 0.533425 7_2:-25603474 Msa1054230:CDS
TAGATCTTGAGGACTTCCAT+AGG 0.534706 7_2:+25603669 None:intergenic
CCCTTCGAGTTCCACCCCGT+TGG 0.536028 7_2:-25603378 Msa1054230:CDS
TGCCCGGAACACGGTGGAGA+CGG 0.536445 7_2:+25603280 None:intergenic
CCAACGGGGTGGAACTCGAA+GGG 0.537973 7_2:+25603378 None:intergenic
CAGGCCATTGGTAGCCCTGA+AGG 0.538080 7_2:+25603532 None:intergenic
AGAATCTCTAAGTGCAAGAC+TGG 0.538771 7_2:-25603793 Msa1054230:CDS
GGACTTCCATAGGGTTTGAT+GGG 0.540263 7_2:+25603679 None:intergenic
GGAATCAGCACCAAGGGCTA+AGG 0.543556 7_2:-25603091 Msa1054230:CDS
ATCCTCAAGTTGTTCTCCAA+CGG 0.543558 7_2:+25603362 None:intergenic
TTGGGTTTGTATTCATGTGA+TGG 0.544918 7_2:-25602901 Msa1054230:CDS
AGACAATTTGGTAGATCTTG+AGG 0.549770 7_2:+25603658 None:intergenic
AAGGTTGTTTGTTGCTTGGG+TGG 0.550029 7_2:-25602858 Msa1054230:CDS
TCCAGTGGAATCAGCACCAA+GGG 0.550198 7_2:-25603097 Msa1054230:CDS
AGATCTTGAGGACTTCCATA+GGG 0.551151 7_2:+25603670 None:intergenic
GAGCAAGAAGCAAGCCATAA+CGG 0.555779 7_2:-25603195 Msa1054230:CDS
TCTAAGTCAAATTTGAGAGA+TGG 0.557349 7_2:+25605110 None:intergenic
TGTAACTATAACTATGCTCA+AGG 0.559054 7_2:-25604948 Msa1054230:CDS
ATCAGCACCAAGGGCTAAGG+TGG 0.559830 7_2:-25603088 Msa1054230:CDS
ATGGAAGGGAAAGGGTTCAA+AGG 0.562746 7_2:-25602967 Msa1054230:CDS
GGTAGCCCTGAAGGATATCA+AGG 0.563155 7_2:+25603541 None:intergenic
GGATATCAAGGTCGATGACA+TGG 0.564199 7_2:+25603553 None:intergenic
CGGAGGAAAGGAGCACCACC+GGG 0.564457 7_2:+25603483 None:intergenic
GGTAGATAAACATGATGAGG+AGG 0.565470 7_2:+25603704 None:intergenic
GAAGGTAGATAAACATGATG+AGG 0.572092 7_2:+25603701 None:intergenic
AAATGGAGGAAGATAATGGA+AGG 0.574690 7_2:-25602982 Msa1054230:CDS
GAGGTGGTGAACAACCTGAT+AGG 0.574741 7_2:+25604910 None:intergenic
CGAGATGATTGCCCGGAACA+CGG 0.575356 7_2:+25603271 None:intergenic
ACGCCGGTGATCCGGAGGAA+AGG 0.576975 7_2:+25603471 None:intergenic
AGGTTCCCGAGATGATTGCC+CGG 0.578504 7_2:+25603264 None:intergenic
CTATTATCGCTGCTTCTGCA+TGG 0.579656 7_2:-25602825 Msa1054230:CDS
ACATGAAGCTACTCTTTGCA+TGG 0.581987 7_2:+25603821 None:intergenic
TCCAACGGGGTGGAACTCGA+AGG 0.584775 7_2:+25603377 None:intergenic
TCCTCCGGATCACCGGCGTA+GGG 0.584832 7_2:-25603467 Msa1054230:CDS
CCTGCATGAATGCAGGCCAT+TGG 0.587372 7_2:+25603520 None:intergenic
GTTTAACCGCCGTGTAGGCG+AGG 0.587763 7_2:-25603325 Msa1054230:CDS
GTGTTACTACTCTATTGAGC+TGG 0.588376 7_2:+25603856 None:intergenic
GTGAATACATGCTAGCAAGA+AGG 0.591113 7_2:-25603887 Msa1054230:CDS
TGTGCTGAAGCAGTGGCAAA+AGG 0.593207 7_2:-25603909 Msa1054230:CDS
CTACTCTATTGAGCTGGTGT+AGG 0.595572 7_2:+25603862 None:intergenic
TGCTGATTCCACTGGAAAAG+TGG 0.596664 7_2:+25603104 None:intergenic
CGGAACATCGTAGCATGTGA+AGG 0.597195 7_2:-25603039 Msa1054230:CDS
GAATTAAATACTCTATGAAG+TGG 0.597232 7_2:+25604825 None:intergenic
GCCGTGCATCGAATCAGTTA+GGG 0.599094 7_2:-25603445 Msa1054230:CDS
CAACGGGGTGGAACTCGAAG+GGG 0.601149 7_2:+25603379 None:intergenic
TCTAGCATGTGCTGAAGCAG+TGG 0.602572 7_2:-25603916 Msa1054230:CDS
ATCACCAGATAGTTCAATGT+GGG 0.605621 7_2:-25605069 Msa1054230:CDS
ATATCCTTCAGGGCTACCAA+TGG 0.606276 7_2:-25603536 Msa1054230:CDS
GCCCTACGCCGGTGATCCGG+AGG 0.610803 7_2:+25603466 None:intergenic
AGGAAGATAATGGAAGGGAA+AGG 0.613642 7_2:-25602976 Msa1054230:CDS
CCCCGTTGGAGAACAACTTG+AGG 0.617458 7_2:-25603364 Msa1054230:CDS
CATCGTAGCATGTGAAGGTG+AGG 0.619286 7_2:-25603034 Msa1054230:CDS
CTTTGCATGGAGTCACCTAG+TGG 0.624018 7_2:+25603834 None:intergenic
GGAGCAGTATATATTTGCAC+CGG 0.624565 7_2:-25603067 Msa1054230:CDS
TTCCAGTGGAATCAGCACCA+AGG 0.632372 7_2:-25603098 Msa1054230:CDS
AATGGAGGAAGATAATGGAA+GGG 0.644464 7_2:-25602981 Msa1054230:CDS
ATTTGACTTAGATGGTGATG+TGG 0.646549 7_2:-25605099 Msa1054230:CDS
AATAGAGTAGTAACACCACT+AGG 0.648126 7_2:-25603849 Msa1054230:CDS
CACATGTTTAACCGCCGTGT+AGG 0.660169 7_2:-25603330 Msa1054230:CDS
CAAGTTGTTCTCCAACGGGG+TGG 0.668903 7_2:+25603367 None:intergenic
ACACGGCGGTTAAACATGTG+CGG 0.674730 7_2:+25603333 None:intergenic
TAGCATGTGAAGGTGAGGAG+AGG 0.683443 7_2:-25603029 Msa1054230:CDS
ACCAAACATAGTGACACTTG+TGG 0.687522 7_2:-25603217 Msa1054230:CDS
GATGACGATGATAACGATGA+TGG 0.694572 7_2:+25603726 None:intergenic
TATATGTAGGAACAAGACAG+TGG 0.702151 7_2:-25603960 Msa1054230:intron
TTATTGTAAGTAGAAGCTTG+TGG 0.706364 7_2:+25604972 None:intergenic
CCAATTTGTGATGCAAAACG+AGG 0.711917 7_2:+25604891 None:intergenic
TGATGGTTATAGGTTAACTG+AGG 0.712462 7_2:-25602884 Msa1054230:CDS
AATCACCAGATAGTTCAATG+TGG 0.716750 7_2:-25605070 Msa1054230:CDS
GATGATTGCCCGGAACACGG+TGG 0.729817 7_2:+25603274 None:intergenic
CCTCAAGTTGTTCTCCAACG+GGG 0.734185 7_2:+25603364 None:intergenic
ATTTGTGATGCAAAACGAGG+TGG 0.735195 7_2:+25604894 None:intergenic
CCAAAGCCTCGCCTACACGG+CGG 0.759718 7_2:+25603319 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAATTAATTAAGATTTT+AGG + chr7_2:25603716-25603735 None:intergenic 10.0%
!! TTAAATATTTAATACAGAAA+TGG + chr7_2:25604004-25604023 None:intergenic 10.0%
!! AATAGTAATAATCAACAAAA+AGG - chr7_2:25603224-25603243 Msa1054230:CDS 15.0%
!! ATAGTAATAATCAACAAAAA+GGG - chr7_2:25603225-25603244 Msa1054230:CDS 15.0%
!! TATGTTAATTTATTCAGTTA+GGG + chr7_2:25603760-25603779 None:intergenic 15.0%
!! TTATGTTAATTTATTCAGTT+AGG + chr7_2:25603761-25603780 None:intergenic 15.0%
!!! TTTGTTTGATTTGAAGAATT+GGG + chr7_2:25602873-25602892 None:intergenic 20.0%
!!! ATTTGTTTGATTTGAAGAAT+TGG + chr7_2:25602874-25602893 None:intergenic 20.0%
!! TCATGAAATCATTATCTAAA+TGG + chr7_2:25603058-25603077 None:intergenic 20.0%
!!! TGTTGCATATATTGATTATT+TGG + chr7_2:25603287-25603306 None:intergenic 20.0%
!! ATATTTAATACAGAAATGGA+AGG + chr7_2:25604000-25604019 None:intergenic 20.0%
!! GCTATATATCTAATATATGT+AGG - chr7_2:25604112-25604131 Msa1054230:intron 20.0%
!! TTGAAGACATTTTTCTTTGA+GGG + chr7_2:25602850-25602869 None:intergenic 25.0%
! TAAATCACTACAAGAATTTG+TGG + chr7_2:25602916-25602935 None:intergenic 25.0%
!! TTAGGGTTAATGTTTCTAAT+TGG + chr7_2:25603089-25603108 None:intergenic 25.0%
! AATAATCAACAAAAAGGGAA+AGG - chr7_2:25603230-25603249 Msa1054230:CDS 25.0%
! GAATTAAATACTCTATGAAG+TGG + chr7_2:25603263-25603282 None:intergenic 25.0%
!! AACTCCAATATCAACAATTT+TGG - chr7_2:25603415-25603434 Msa1054230:CDS 25.0%
! CTATCCAAAATTGTTGATAT+TGG + chr7_2:25603422-25603441 None:intergenic 25.0%
! AGTGATATGTATCATCAAAT+GGG - chr7_2:25603539-25603558 Msa1054230:CDS 25.0%
! TTAATTTATTCAGTTAGGGA+GGG + chr7_2:25603756-25603775 None:intergenic 25.0%
! ATTGGTTATGTAATCCATAA+AGG - chr7_2:25603946-25603965 Msa1054230:CDS 25.0%
!! AGTGTGCAAATTTTATGTAA+GGG + chr7_2:25604463-25604482 None:intergenic 25.0%
!! AAGTGTGCAAATTTTATGTA+AGG + chr7_2:25604464-25604483 None:intergenic 25.0%
! GTTGAAGACATTTTTCTTTG+AGG + chr7_2:25602851-25602870 None:intergenic 30.0%
ATTTGAGAGATGGTAAGTTT+AGG + chr7_2:25602968-25602987 None:intergenic 30.0%
TCTAAGTCAAATTTGAGAGA+TGG + chr7_2:25602978-25602997 None:intergenic 30.0%
! TCTCTCAAATTTGACTTAGA+TGG - chr7_2:25602978-25602997 Msa1054230:CDS 30.0%
TTATTGTAAGTAGAAGCTTG+TGG + chr7_2:25603116-25603135 None:intergenic 30.0%
TGTAACTATAACTATGCTCA+AGG - chr7_2:25603137-25603156 Msa1054230:CDS 30.0%
!! TGATCACTTTTCATCAACAA+AGG - chr7_2:25603367-25603386 Msa1054230:CDS 30.0%
!!! TTCTTCTGAATGTTTTGACA+TGG - chr7_2:25603391-25603410 Msa1054230:CDS 30.0%
CAAAATTCTTCAAGCTATTG+TGG - chr7_2:25603452-25603471 Msa1054230:CDS 30.0%
! GAAGAATCTACTATTTTGCA+TGG - chr7_2:25603485-25603504 Msa1054230:CDS 30.0%
GAGTGATATGTATCATCAAA+TGG - chr7_2:25603538-25603557 Msa1054230:CDS 30.0%
!! TTTTAGGTGAAGAAAGACAA+AGG + chr7_2:25603700-25603719 None:intergenic 30.0%
TAATTTATTCAGTTAGGGAG+GGG + chr7_2:25603755-25603774 None:intergenic 30.0%
GTTAATTTATTCAGTTAGGG+AGG + chr7_2:25603757-25603776 None:intergenic 30.0%
TGTAATCCATAAAGGAATAG+TGG - chr7_2:25603954-25603973 Msa1054230:CDS 30.0%
ACGCAATCAAGGATATTATT+GGG - chr7_2:25605166-25605185 Msa1054230:CDS 30.0%
TGTATTCATGTGATGGTTAT+AGG - chr7_2:25605191-25605210 Msa1054230:CDS 30.0%
TATAGGTTAACTGAGGATAA+AGG - chr7_2:25605208-25605227 Msa1054230:CDS 30.0%
! ATAAAGGTTGTTTGTTGCTT+GGG - chr7_2:25605224-25605243 Msa1054230:CDS 30.0%
! ATTTGACTTAGATGGTGATG+TGG - chr7_2:25602986-25603005 Msa1054230:CDS 35.0%
ACATTGAACTATCTGGTGAT+TGG + chr7_2:25603016-25603035 None:intergenic 35.0%
AATCACCAGATAGTTCAATG+TGG - chr7_2:25603015-25603034 Msa1054230:CDS 35.0%
ATCACCAGATAGTTCAATGT+GGG - chr7_2:25603016-25603035 Msa1054230:CDS 35.0%
TAGAAGCTTGTGGTGAATTA+GGG + chr7_2:25603106-25603125 None:intergenic 35.0%
ATGCAAATTCAAAGCCTATC+AGG - chr7_2:25603161-25603180 Msa1054230:CDS 35.0%
! GAGTTCTTGGAAGATTTTCA+AGG - chr7_2:25603338-25603357 Msa1054230:CDS 35.0%
! GAATCTACTATTTTGCATGG+TGG - chr7_2:25603488-25603507 Msa1054230:CDS 35.0%
GTATCATCAAATGGGATCTA+TGG - chr7_2:25603547-25603566 Msa1054230:CDS 35.0%
! GCATCTTTTGATGTTTCTCT+TGG + chr7_2:25603613-25603632 None:intergenic 35.0%
CTAACAGTTCCTATTCCAAT+TGG - chr7_2:25603650-25603669 Msa1054230:CDS 35.0%
TGCTCAATTCCAATTGGAAT+AGG + chr7_2:25603662-25603681 None:intergenic 35.0%
TATTCCAATTGGAATTGAGC+AGG - chr7_2:25603661-25603680 Msa1054230:CDS 35.0%
ATTACCTGCTCAATTCCAAT+TGG + chr7_2:25603668-25603687 None:intergenic 35.0%
! GTCTAAAGTCATGATTGATG+CGG - chr7_2:25603831-25603850 Msa1054230:CDS 35.0%
GGACGTTAGATTTACGATAT+TGG - chr7_2:25603928-25603947 Msa1054230:CDS 35.0%
TATATGTAGGAACAAGACAG+TGG - chr7_2:25604125-25604144 Msa1054230:intron 35.0%
AATAGAGTAGTAACACCACT+AGG - chr7_2:25604236-25604255 Msa1054230:intron 35.0%
!! TGATGATGACTTGGTTGTTA+GGG + chr7_2:25604324-25604343 None:intergenic 35.0%
!! TTGATGATGACTTGGTTGTT+AGG + chr7_2:25604325-25604344 None:intergenic 35.0%
!!! CTTTTTGGTTGATGATGACT+TGG + chr7_2:25604333-25604352 None:intergenic 35.0%
!! CGATGATGGTGTTAACTTTT+TGG + chr7_2:25604348-25604367 None:intergenic 35.0%
GAAGGTAGATAAACATGATG+AGG + chr7_2:25604387-25604406 None:intergenic 35.0%
AGACAATTTGGTAGATCTTG+AGG + chr7_2:25604430-25604449 None:intergenic 35.0%
! ATTTTATGTAAGGGCAAGCT+TGG + chr7_2:25604454-25604473 None:intergenic 35.0%
ATATATTTGCACCGGAGATT+CGG - chr7_2:25605026-25605045 Msa1054230:CDS 35.0%
!! AAATGGAGGAAGATAATGGA+AGG - chr7_2:25605103-25605122 Msa1054230:CDS 35.0%
!! AATGGAGGAAGATAATGGAA+GGG - chr7_2:25605104-25605123 Msa1054230:CDS 35.0%
GACGCAATCAAGGATATTAT+TGG - chr7_2:25605165-25605184 Msa1054230:CDS 35.0%
TTGGGTTTGTATTCATGTGA+TGG - chr7_2:25605184-25605203 Msa1054230:CDS 35.0%
TGATGGTTATAGGTTAACTG+AGG - chr7_2:25605201-25605220 Msa1054230:CDS 35.0%
! GATAAAGGTTGTTTGTTGCT+TGG - chr7_2:25605223-25605242 Msa1054230:CDS 35.0%
GATGGTAAGTTTAGGCTTTG+TGG + chr7_2:25602960-25602979 None:intergenic 40.0%
GTAGAAGCTTGTGGTGAATT+AGG + chr7_2:25603107-25603126 None:intergenic 40.0%
ATTTGTGATGCAAAACGAGG+TGG + chr7_2:25603194-25603213 None:intergenic 40.0%
CCAATTTGTGATGCAAAACG+AGG + chr7_2:25603197-25603216 None:intergenic 40.0%
! CCTCGTTTTGCATCACAAAT+TGG - chr7_2:25603194-25603213 Msa1054230:CDS 40.0%
TCCAAGAACTCTTCAATAGC+TGG + chr7_2:25603329-25603348 None:intergenic 40.0%
CACAAGCTCTACAACAAGAA+AGG - chr7_2:25603585-25603604 Msa1054230:CDS 40.0%
TGTTGTGTCCTCTATGTCTA+TGG - chr7_2:25603907-25603926 Msa1054230:CDS 40.0%
TCTAACGTCCATAGACATAG+AGG + chr7_2:25603918-25603937 None:intergenic 40.0%
GTGAATACATGCTAGCAAGA+AGG - chr7_2:25604198-25604217 Msa1054230:intron 40.0%
! GTGTTACTACTCTATTGAGC+TGG + chr7_2:25604232-25604251 None:intergenic 40.0%
ACATGAAGCTACTCTTTGCA+TGG + chr7_2:25604267-25604286 None:intergenic 40.0%
AGAATCTCTAAGTGCAAGAC+TGG - chr7_2:25604292-25604311 Msa1054230:intron 40.0%
! GATGACGATGATAACGATGA+TGG + chr7_2:25604362-25604381 None:intergenic 40.0%
GGTAGATAAACATGATGAGG+AGG + chr7_2:25604384-25604403 None:intergenic 40.0%
AGATCTTGAGGACTTCCATA+GGG + chr7_2:25604418-25604437 None:intergenic 40.0%
TAGATCTTGAGGACTTCCAT+AGG + chr7_2:25604419-25604438 None:intergenic 40.0%
ATCGACCTTGATATCCTTCA+GGG - chr7_2:25604539-25604558 Msa1054230:CDS 40.0%
ATCGAATCAGTTAGGGAAAC+CGG - chr7_2:25604647-25604666 Msa1054230:CDS 40.0%
! ATCCTCAAGTTGTTCTCCAA+CGG + chr7_2:25604726-25604745 None:intergenic 40.0%
ACCAAACATAGTGACACTTG+TGG - chr7_2:25604868-25604887 Msa1054230:CDS 40.0%
! TCCACAAGTGTCACTATGTT+TGG + chr7_2:25604872-25604891 None:intergenic 40.0%
AGAAATCGGCCCAAAAAGTA+CGG + chr7_2:25604917-25604936 None:intergenic 40.0%
AATGCAAAGCCTCGAGAAAT+CGG + chr7_2:25604931-25604950 None:intergenic 40.0%
GGAGCAGTATATATTTGCAC+CGG - chr7_2:25605018-25605037 Msa1054230:CDS 40.0%
GGAGAAATGGAGGAAGATAA+TGG - chr7_2:25605099-25605118 Msa1054230:CDS 40.0%
!! AGGAAGATAATGGAAGGGAA+AGG - chr7_2:25605109-25605128 Msa1054230:CDS 40.0%
!! GGAAGATAATGGAAGGGAAA+GGG - chr7_2:25605110-25605129 Msa1054230:CDS 40.0%
GCTTCCCACATTGAACTATC+TGG + chr7_2:25603023-25603042 None:intergenic 45.0%
GCCAGCTATTGAAGAGTTCT+TGG - chr7_2:25603325-25603344 Msa1054230:CDS 45.0%
CTACTCTATTGAGCTGGTGT+AGG + chr7_2:25604226-25604245 None:intergenic 45.0%
TACCTTCCCATCAAACCCTA+TGG - chr7_2:25604400-25604419 Msa1054230:intron 45.0%
TTCCATAGGGTTTGATGGGA+AGG + chr7_2:25604405-25604424 None:intergenic 45.0%
GGACTTCCATAGGGTTTGAT+GGG + chr7_2:25604409-25604428 None:intergenic 45.0%
AGGACTTCCATAGGGTTTGA+TGG + chr7_2:25604410-25604429 None:intergenic 45.0%
GGCAAGCTTGGTAGACAATT+TGG + chr7_2:25604442-25604461 None:intergenic 45.0%
!! CTCGAAAATGGCTTGGTTAG+CGG + chr7_2:25604489-25604508 None:intergenic 45.0%
TGCTTCAAACGCCTCGAAAA+TGG + chr7_2:25604501-25604520 None:intergenic 45.0%
GGATATCAAGGTCGATGACA+TGG + chr7_2:25604535-25604554 None:intergenic 45.0%
CATCGACCTTGATATCCTTC+AGG - chr7_2:25604538-25604557 Msa1054230:CDS 45.0%
ATATCCTTCAGGGCTACCAA+TGG - chr7_2:25604549-25604568 Msa1054230:CDS 45.0%
!! TCCCTAACTGATTCGATGCA+CGG + chr7_2:25604644-25604663 None:intergenic 45.0%
! TAACTCCGTTAAGCATCGTC+CGG + chr7_2:25604669-25604688 None:intergenic 45.0%
! TCCTCAAGTTGTTCTCCAAC+GGG + chr7_2:25604725-25604744 None:intergenic 45.0%
GGTCTCTTATCATGGACAAC+AGG + chr7_2:25604844-25604863 None:intergenic 45.0%
TGGTGCTTGGTCTCTTATCA+TGG + chr7_2:25604852-25604871 None:intergenic 45.0%
!! GTGTCACTATGTTTGGTGCT+TGG + chr7_2:25604865-25604884 None:intergenic 45.0%
GAGCAAGAAGCAAGCCATAA+CGG - chr7_2:25604890-25604909 Msa1054230:CDS 45.0%
AGCAAGAAGCAAGCCATAAC+GGG - chr7_2:25604891-25604910 Msa1054230:CDS 45.0%
!!! CATAACGGGCCGTACTTTTT+GGG - chr7_2:25604905-25604924 Msa1054230:CDS 45.0%
! CTCTGCAATTTTCGACTCCT+TGG - chr7_2:25604955-25604974 Msa1054230:CDS 45.0%
!! TGCTGATTCCACTGGAAAAG+TGG + chr7_2:25604984-25605003 None:intergenic 45.0%
ATGAGAGGTTGGAGAAATGG+AGG - chr7_2:25605089-25605108 Msa1054230:CDS 45.0%
ATGGAAGGGAAAGGGTTCAA+AGG - chr7_2:25605118-25605137 Msa1054230:CDS 45.0%
GTCACTGCATTAGGACTTAG+AGG + chr7_2:25605148-25605167 None:intergenic 45.0%
CTTGATTGCGTCACTGCATT+AGG + chr7_2:25605157-25605176 None:intergenic 45.0%
CTAATGCAGTGACGCAATCA+AGG - chr7_2:25605155-25605174 Msa1054230:CDS 45.0%
! AAGGTTGTTTGTTGCTTGGG+TGG - chr7_2:25605227-25605246 Msa1054230:CDS 45.0%
CTATTATCGCTGCTTCTGCA+TGG - chr7_2:25605260-25605279 Msa1054230:CDS 45.0%
GAGGTGGTGAACAACCTGAT+AGG + chr7_2:25603178-25603197 None:intergenic 50.0%
GCTGCGCCACTATTCCTTTA+TGG + chr7_2:25603963-25603982 None:intergenic 50.0%
TCTAGCATGTGCTGAAGCAG+TGG - chr7_2:25604169-25604188 Msa1054230:intron 50.0%
TGTGCTGAAGCAGTGGCAAA+AGG - chr7_2:25604176-25604195 Msa1054230:intron 50.0%
CTTTGCATGGAGTCACCTAG+TGG + chr7_2:25604254-25604273 None:intergenic 50.0%
!! CGCTAACCAAGCCATTTTCG+AGG - chr7_2:25604487-25604506 Msa1054230:CDS 50.0%
CAAACGCCTCGAAAATGGCT+TGG + chr7_2:25604496-25604515 None:intergenic 50.0%
GGTAGCCCTGAAGGATATCA+AGG + chr7_2:25604547-25604566 None:intergenic 50.0%
GCCGTGCATCGAATCAGTTA+GGG - chr7_2:25604640-25604659 Msa1054230:CDS 50.0%
GGAAACCGGACGATGCTTAA+CGG - chr7_2:25604661-25604680 Msa1054230:CDS 50.0%
! CCTCAAGTTGTTCTCCAACG+GGG + chr7_2:25604724-25604743 None:intergenic 50.0%
ACACGGCGGTTAAACATGTG+CGG + chr7_2:25604755-25604774 None:intergenic 50.0%
CACATGTTTAACCGCCGTGT+AGG - chr7_2:25604755-25604774 Msa1054230:CDS 50.0%
AACACGGTGGAGACGGTTAA+CGG + chr7_2:25604801-25604820 None:intergenic 50.0%
! CCAAAAAGTACGGCCCGTTA+TGG + chr7_2:25604907-25604926 None:intergenic 50.0%
!!! CCATAACGGGCCGTACTTTT+TGG - chr7_2:25604904-25604923 Msa1054230:CDS 50.0%
!! CTTTTTGGGCCGATTTCTCG+AGG - chr7_2:25604919-25604938 Msa1054230:CDS 50.0%
TTCCAGTGGAATCAGCACCA+AGG - chr7_2:25604987-25605006 Msa1054230:CDS 50.0%
TCCAGTGGAATCAGCACCAA+GGG - chr7_2:25604988-25605007 Msa1054230:CDS 50.0%
GCTACGATGTTCCGAATCTC+CGG + chr7_2:25605040-25605059 None:intergenic 50.0%
CGGAACATCGTAGCATGTGA+AGG - chr7_2:25605046-25605065 Msa1054230:CDS 50.0%
CATCGTAGCATGTGAAGGTG+AGG - chr7_2:25605051-25605070 Msa1054230:CDS 50.0%
TAGCATGTGAAGGTGAGGAG+AGG - chr7_2:25605056-25605075 Msa1054230:CDS 50.0%
AAGGTGAGGAGAGGATAGAG+AGG - chr7_2:25605065-25605084 Msa1054230:CDS 50.0%
! AGAGGATAGAGAGGCATGAG+AGG - chr7_2:25605074-25605093 Msa1054230:CDS 50.0%
! GATAGAGAGGCATGAGAGGT+TGG - chr7_2:25605078-25605097 Msa1054230:CDS 50.0%
GGCATGAGAGGTTGGAGAAA+TGG - chr7_2:25605086-25605105 Msa1054230:CDS 50.0%
CCTGCATGAATGCAGGCCAT+TGG + chr7_2:25604568-25604587 None:intergenic 55.0%
CCAATGGCCTGCATTCATGC+AGG - chr7_2:25604565-25604584 Msa1054230:CDS 55.0%
GCCTGCATTCATGCAGGCAT+TGG - chr7_2:25604571-25604590 Msa1054230:CDS 55.0%
GCCAATGCCTGCATGAATGC+AGG + chr7_2:25604575-25604594 None:intergenic 55.0%
!! TCATGCAGGCATTGGCTGCA+CGG - chr7_2:25604579-25604598 Msa1054230:CDS 55.0%
GGCCGTGCATCGAATCAGTT+AGG - chr7_2:25604639-25604658 Msa1054230:CDS 55.0%
CAAGTTGTTCTCCAACGGGG+TGG + chr7_2:25604721-25604740 None:intergenic 55.0%
CCCCGTTGGAGAACAACTTG+AGG - chr7_2:25604721-25604740 Msa1054230:CDS 55.0%
TAACCGTCTCCACCGTGTTC+CGG - chr7_2:25604802-25604821 Msa1054230:CDS 55.0%
CGAGATGATTGCCCGGAACA+CGG + chr7_2:25604817-25604836 None:intergenic 55.0%
CGTGTTCCGGGCAATCATCT+CGG - chr7_2:25604815-25604834 Msa1054230:CDS 55.0%
GTGTTCCGGGCAATCATCTC+GGG - chr7_2:25604816-25604835 Msa1054230:CDS 55.0%
AGGTTCCCGAGATGATTGCC+CGG + chr7_2:25604824-25604843 None:intergenic 55.0%
CACTGGAAAAGTGGCGTCCA+AGG + chr7_2:25604975-25604994 None:intergenic 55.0%
!!! CTTGGACGCCACTTTTCCAG+TGG - chr7_2:25604973-25604992 Msa1054230:CDS 55.0%
GGAATCAGCACCAAGGGCTA+AGG - chr7_2:25604994-25605013 Msa1054230:CDS 55.0%
ATCAGCACCAAGGGCTAAGG+TGG - chr7_2:25604997-25605016 Msa1054230:CDS 55.0%
TACTGCTCCACCTTAGCCCT+TGG + chr7_2:25605007-25605026 None:intergenic 55.0%
! CAGGCCATTGGTAGCCCTGA+AGG + chr7_2:25604556-25604575 None:intergenic 60.0%
GCTCCTTTCCTCCGGATCAC+CGG - chr7_2:25604611-25604630 Msa1054230:CDS 60.0%
CAACGGGGTGGAACTCGAAG+GGG + chr7_2:25604709-25604728 None:intergenic 60.0%
CCAACGGGGTGGAACTCGAA+GGG + chr7_2:25604710-25604729 None:intergenic 60.0%
TCCAACGGGGTGGAACTCGA+AGG + chr7_2:25604711-25604730 None:intergenic 60.0%
GTTTAACCGCCGTGTAGGCG+AGG - chr7_2:25604760-25604779 Msa1054230:CDS 60.0%
AACCGTCTCCACCGTGTTCC+GGG - chr7_2:25604803-25604822 Msa1054230:CDS 60.0%
GATGATTGCCCGGAACACGG+TGG + chr7_2:25604814-25604833 None:intergenic 60.0%
!! GCCCTTGGTGCTGATTCCAC+TGG + chr7_2:25604992-25605011 None:intergenic 60.0%
!! CGGAGGAAAGGAGCACCACC+GGG + chr7_2:25604605-25604624 None:intergenic 65.0%
!! CCGGAGGAAAGGAGCACCAC+CGG + chr7_2:25604606-25604625 None:intergenic 65.0%
CCGGTGGTGCTCCTTTCCTC+CGG - chr7_2:25604603-25604622 Msa1054230:CDS 65.0%
ACGCCGGTGATCCGGAGGAA+AGG + chr7_2:25604617-25604636 None:intergenic 65.0%
TTCCTCCGGATCACCGGCGT+AGG - chr7_2:25604617-25604636 Msa1054230:CDS 65.0%
TCCTCCGGATCACCGGCGTA+GGG - chr7_2:25604618-25604637 Msa1054230:CDS 65.0%
TTCGATGCACGGCCCTACGC+CGG + chr7_2:25604633-25604652 None:intergenic 65.0%
! CCCTTCGAGTTCCACCCCGT+TGG - chr7_2:25604707-25604726 Msa1054230:CDS 65.0%
CCAAAGCCTCGCCTACACGG+CGG + chr7_2:25604769-25604788 None:intergenic 65.0%
CCGCCAAAGCCTCGCCTACA+CGG + chr7_2:25604772-25604791 None:intergenic 65.0%
! CCGTGTAGGCGAGGCTTTGG+CGG - chr7_2:25604769-25604788 Msa1054230:CDS 65.0%
TGCCCGGAACACGGTGGAGA+CGG + chr7_2:25604808-25604827 None:intergenic 65.0%
!! CAGGCATTGGCTGCACGGCC+CGG - chr7_2:25604584-25604603 Msa1054230:CDS 70.0%
ACGGCCCTACGCCGGTGATC+CGG + chr7_2:25604625-25604644 None:intergenic 70.0%
CCGCCGTGTAGGCGAGGCTT+TGG - chr7_2:25604766-25604785 Msa1054230:CDS 70.0%
!! GCATTGGCTGCACGGCCCGG+TGG - chr7_2:25604587-25604606 Msa1054230:CDS 75.0%
GCCCTACGCCGGTGATCCGG+AGG + chr7_2:25604622-25604641 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 25602816 25605291 25602816 ID=Msa1054230;Name=Msa1054230
chr7_2 mRNA 25602816 25605291 25602816 ID=Msa1054230-mRNA-1;Parent=Msa1054230;Name=Msa1054230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|674
chr7_2 exon 25604425 25605291 25604425 ID=Msa1054230-mRNA-1:exon:5517;Parent=Msa1054230-mRNA-1
chr7_2 exon 25602816 25603973 25602816 ID=Msa1054230-mRNA-1:exon:5516;Parent=Msa1054230-mRNA-1
chr7_2 CDS 25604425 25605291 25604425 ID=Msa1054230-mRNA-1:cds;Parent=Msa1054230-mRNA-1
chr7_2 CDS 25602816 25603973 25602816 ID=Msa1054230-mRNA-1:cds;Parent=Msa1054230-mRNA-1
Gene Sequence

>Msa1054230

ATGATGAATTCACTTTGTGGAAGCTCAAATTCCCTCAAAGAAAAATGTCTTCAACCCAATTCTTCAAATCAAACAAATACTCACTCAAAGAAAAATGCCACAAATTCTTGTAGTGATTTAGAGCAAATTAATGTTCTAACTCCACAAAGCCTAAACTTACCATCTCTCAAATTTGACTTAGATGGTGATGTGGAAGTCCAATCACCAGATAGTTCAATGTGGGAAGCATTTTTCAATGACCATTTAGATAATGATTTCATGATATCATCACCAATTAGAAACATTAACCCTAATTCACCACAAGCTTCTACTTACAATAATTGTAACTATAACTATGCTCAAGGCATGCAAATTCAAAGCCTATCAGGTTGTTCACCACCTCGTTTTGCATCACAAATTGGTTCATTAAATAGTAATAATCAACAAAAAGGGAAAGGACTTAGTCCACTTCATAGAGTATTTAATTCACCAAATAATCAATATATGCAACATGTTGAAAATCTTTCATTGCCAGCTATTGAAGAGTTCTTGGAAGATTTTCAAGGTGATGTTGATCACTTTTCATCAACAAAGGTTTCTTCTGAATGTTTTGACATGGAAACTCCAATATCAACAATTTTGGATAGTTTAACAATGCAAAATTCTTCAAGCTATTGTGGTTCAGTTAATGAAGAATCTACTATTTTGCATGGTGGTAATTCTTCTTCTCAACTTTCTCAAGAGAGTGATATGTATCATCAAATGGGATCTATGGCTAGTGCTTCTCTTTCACAAGCTCTACAACAAGAAAGGTACCAAGAGAAACATCAAAAGATGCAAGCTCAACAACAGAGTCTAACAGTTCCTATTCCAATTGGAATTGAGCAGGAACAAGACAGTGGACTTCAACTAGTGCACTTACTTCTAGCATGTGCTGAAGCAGTGGCAAAAGGTGAATACATGCTAGCAAGAAGGTACCTACACCAGCTCAATAGAGTAGTAACACCACTAGGTGACTCCATGCAAAGAGTAGCTTCATGTTTCACAGAATCTCTAAGTGCAAGACTGGCTGCAACCCTAACAACCAAGTCATCATCAACCAAAAAGTTAACACCATCATCGTTATCATCGTCATCCTCCTCATCATGTTTATCTACCTTCCCATCAAACCCTATGGAAGTCCTCAAGATCTACCAAATTGTCTACCAAGCTTGCCCTTACATAAAATTTGCACACTTTACCGCTAACCAAGCCATTTTCGAGGCGTTTGAAGCAGAAGAGCGTGTCCATGTCATCGACCTTGATATCCTTCAGGGCTACCAATGGCCTGCATTCATGCAGGCATTGGCTGCACGGCCCGGTGGTGCTCCTTTCCTCCGGATCACCGGCGTAGGGCCGTGCATCGAATCAGTTAGGGAAACCGGACGATGCTTAACGGAGTTAGCTCATTCTCTTCGTATCCCCTTCGAGTTCCACCCCGTTGGAGAACAACTTGAGGATCTTAAACCGCACATGTTTAACCGCCGTGTAGGCGAGGCTTTGGCGGTTAACACCGTTAACCGTCTCCACCGTGTTCCGGGCAATCATCTCGGGAACCTGTTGTCCATGATAAGAGACCAAGCACCAAACATAGTGACACTTGTGGAGCAAGAAGCAAGCCATAACGGGCCGTACTTTTTGGGCCGATTTCTCGAGGCTTTGCATTACTACTCTGCAATTTTCGACTCCTTGGACGCCACTTTTCCAGTGGAATCAGCACCAAGGGCTAAGGTGGAGCAGTATATATTTGCACCGGAGATTCGGAACATCGTAGCATGTGAAGGTGAGGAGAGGATAGAGAGGCATGAGAGGTTGGAGAAATGGAGGAAGATAATGGAAGGGAAAGGGTTCAAAGGAGTGCCTCTAAGTCCTAATGCAGTGACGCAATCAAGGATATTATTGGGTTTGTATTCATGTGATGGTTATAGGTTAACTGAGGATAAAGGTTGTTTGTTGCTTGGGTGGCAAGACAGAGCTATTATCGCTGCTTCTGCATGGCGATGCTGA
Protein sequence

>Msa1054230

MMNSLCGSSNSLKEKCLQPNSSNQTNTHSKKNATNSCSDLEQINVLTPQSLNLPSLKFDLDGDVEVQSPDSSMWEAFFNDHLDNDFMISSPIRNINPNSPQASTYNNCNYNYAQGMQIQSLSGCSPPRFASQIGSLNSNNQQKGKGLSPLHRVFNSPNNQYMQHVENLSLPAIEEFLEDFQGDVDHFSSTKVSSECFDMETPISTILDSLTMQNSSSYCGSVNEESTILHGGNSSSQLSQESDMYHQMGSMASASLSQALQQERYQEKHQKMQAQQQSLTVPIPIGIEQEQDSGLQLVHLLLACAEAVAKGEYMLARRYLHQLNRVVTPLGDSMQRVASCFTESLSARLAATLTTKSSSTKKLTPSSLSSSSSSSCLSTFPSNPMEVLKIYQIVYQACPYIKFAHFTANQAIFEAFEAEERVHVIDLDILQGYQWPAFMQALAARPGGAPFLRITGVGPCIESVRETGRCLTELAHSLRIPFEFHPVGEQLEDLKPHMFNRRVGEALAVNTVNRLHRVPGNHLGNLLSMIRDQAPNIVTLVEQEASHNGPYFLGRFLEALHYYSAIFDSLDATFPVESAPRAKVEQYIFAPEIRNIVACEGEERIERHERLEKWRKIMEGKGFKGVPLSPNAVTQSRILLGLYSCDGYRLTEDKGCLLLGWQDRAIIAASAWRC*