AlfalfaGEDB | Msa1059720

M. sativa cultivar zhongmu-4 / Msa1059720

KEGG: K04733
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059720	PNX55140.1	66.667	147	15	2	1	113	36	182	1.49e-54	179

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059720	sp\|Q9LPZ9\|SD113_ARATH	40.268	149	55	3	1	115	681	829	2.22e-22	94.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059720	A0A2K3JM84	66.667	147	15	2	1	113	36	182	7.13e-55	179

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059720	MtrunA17_Chr7g0236691	57.931	145	27	2	1	111	670	814	7.02e-44	152
Msa1059720	MtrunA17_Chr7g0236471	55.705	149	32	2	1	115	510	658	7.22e-44	151
Msa1059720	MtrunA17_Chr7g0236531	54.422	147	33	2	1	113	208	354	1.54e-43	145
Msa1059720	MtrunA17_Chr7g0236571	57.639	144	27	2	6	115	510	653	3.48e-43	149
Msa1059720	MtrunA17_Chr4g0055011	55.782	147	31	2	1	113	241	387	7.69e-42	141
Msa1059720	MtrunA17_Chr7g0236551	55.782	147	31	2	1	113	658	804	1.20e-41	145
Msa1059720	MtrunA17_Chr7g0236341	56.944	144	25	3	1	111	688	827	3.56e-41	144
Msa1059720	MtrunA17_Chr7g0236641	55.102	147	32	2	1	113	695	841	1.82e-40	142
Msa1059720	MtrunA17_Chr7g0236201	53.741	147	34	3	1	113	640	786	2.54e-40	142
Msa1059720	MtrunA17_Chr7g0236121	53.676	136	30	2	1	103	674	809	1.19e-38	137

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059720	AT1G11350.1	40.268	149	55	3	1	115	681	829	2.26e-23	94.0
Msa1059720	AT1G11330.2	38.926	149	57	3	1	115	693	841	1.59e-22	91.7
Msa1059720	AT1G11330.1	38.926	149	57	3	1	115	691	839	1.67e-22	91.7
Msa1059720	AT1G11300.1	41.259	143	55	2	1	115	678	819	3.66e-22	90.5
Msa1059720	AT1G11300.2	41.259	143	55	2	1	115	663	804	4.03e-22	90.5
Msa1059720	AT1G11303.1	41.007	139	53	3	1	111	678	815	1.00e-19	83.6
Msa1059720	AT1G61610.1	36.424	151	57	5	1	115	694	841	4.60e-19	81.6
Msa1059720	AT4G23150.1	37.241	145	55	4	1	110	505	648	1.33e-18	80.5
Msa1059720	AT4G21380.1	57.143	70	24	1	1	64	695	764	6.15e-18	78.6
Msa1059720	AT1G65790.1	32.903	155	64	4	1	115	688	842	7.75e-18	78.2

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Find 18 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGGTTGACTAGGAGG+AGG	0.588090	7_2:+46470016	None:intergenic
AATTCAACATATTCTGCCTT+CGG	0.411991	7_2:+46469990	None:intergenic
AGTATTACATACATAGTGGC+AGG	0.561252	7_2:-46469920	Msa1059720:CDS
ATGAAAGCAGGTTGACTAGG+AGG	0.603846	7_2:+46470013	None:intergenic
CGGATGAAAGCAGGTTGACT+AGG	0.521190	7_2:+46470010	None:intergenic
GTCAACCTGCTTTCATCCGA+AGG	0.564262	7_2:-46470006	Msa1059720:CDS
GTCTGGATACAATGGCAGGA+AGG	0.618922	7_2:-46470201	Msa1059720:CDS
GTGATTATAATCATGTTTCA+AGG	0.450808	7_2:-46470137	Msa1059720:intron
TATATTCCTGGATCTATCAA+AGG	0.578044	7_2:+46470168	None:intergenic
TCACTATGATCATATATTCC+TGG	0.413256	7_2:+46470156	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATATTCCTGGATCTATCAA+AGG	+	chr7_2:46470166-46470185	None:intergenic	30.0%
TCACTATGATCATATATTCC+TGG	+	chr7_2:46470178-46470197	None:intergenic	30.0%
TTTCAAGGTGCATACATATA+GGG	-	chr7_2:46470209-46470228	Msa1059720:intron	30.0%
AATTCAACATATTCTGCCTT+CGG	+	chr7_2:46470344-46470363	None:intergenic	30.0%
TGTCAGTATTACATACATAG+TGG	-	chr7_2:46470407-46470426	Msa1059720:CDS	30.0%
TCTCTTGAATATGCAATGCA+AGG	-	chr7_2:46469910-46469929	Msa1059720:CDS	35.0%
TTGATGCTAGAGATCATTAG+TGG	-	chr7_2:46469973-46469992	Msa1059720:CDS	35.0%
TGATGCTAGAGATCATTAGT+GGG	-	chr7_2:46469974-46469993	Msa1059720:CDS	35.0%
TAGTCAGAAACGAACTATTG+AGG	+	chr7_2:46470041-46470060	None:intergenic	35.0%
ATTGTATCTGCAAACGGAAA+AGG	+	chr7_2:46470075-46470094	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	46469907	46470446	46469907	ID=Msa1059720;Name=Msa1059720
chr7_2	mRNA	46469907	46470446	46469907	ID=Msa1059720-mRNA-1;Parent=Msa1059720;Name=Msa1059720-mRNA-1;_AED=0.67;_eAED=0.67;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|119
chr7_2	exon	46470321	46470446	46470321	ID=Msa1059720-mRNA-1:exon:8422;Parent=Msa1059720-mRNA-1
chr7_2	exon	46470138	46470223	46470138	ID=Msa1059720-mRNA-1:exon:8421;Parent=Msa1059720-mRNA-1
chr7_2	exon	46469907	46470054	46469907	ID=Msa1059720-mRNA-1:exon:8420;Parent=Msa1059720-mRNA-1
chr7_2	CDS	46470321	46470446	46470321	ID=Msa1059720-mRNA-1:cds;Parent=Msa1059720-mRNA-1
chr7_2	CDS	46470138	46470223	46470138	ID=Msa1059720-mRNA-1:cds;Parent=Msa1059720-mRNA-1
chr7_2	CDS	46469907	46470054	46469907	ID=Msa1059720-mRNA-1:cds;Parent=Msa1059720-mRNA-1

Gene Sequence	>Msa1059720 ATGTCTCTTGAATATGCAATGCAAGGACTATTTTCGGAGAAATCAGATGTTTTTAGCTTTGGTGTTTTGATGCTAGAGATCATTAGTGGGAGAAGAAACTCAAGCTTTTATGACAATGAGAATGCTGTCTGGATACAATGGCAGGAAGGCAGTATTTTACCTTTGATAGATCCAGGAATATATGATCATAGTGATTATAATCATGTTTCAAGTGAGGCTGCATTTCTTCCTCCTCCTAGTCAACCTGCTTTCATCCGAAGGCAGAATATGTTGAATTCGAAGTCACCTGATGAAAATCAAAACGATCTATCCATCAATACTGTCAGTATTACATACATAGTGGCAGGTAGCGCACTATAA
Protein sequence	>Msa1059720 MSLEYAMQGLFSEKSDVFSFGVLMLEIISGRRNSSFYDNENAVWIQWQEGSILPLIDPGIYDHSDYNHVSSEAAFLPPPSQPAFIRRQNMLNSKSPDENQNDLSINTVSITYIVAGSAL*