AlfalfaGEDB | Msa1063130

M. sativa cultivar zhongmu-4 / Msa1063130

KEGG: K10802|K11296
GO: GO:0000785 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003700 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006333 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030527 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00505.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063130	XP_003623293.1	98.214	168	3	0	1	168	1	168	9.05e-109	317

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063130	sp\|O49595\|HMGB1_ARATH	70.548	146	40	1	3	148	5	147	4.13e-62	192

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063130	G7KS90	98.214	168	3	0	1	168	1	168	4.32e-109	317

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TFs/TRs:

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Gene ID	Type	Classification
Msa1063130	TR	HMG

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063130	MtrunA17_Chr7g0242251	98.214	168	3	0	1	168	1	168	8.32e-113	317
Msa1063130	MtrunA17_Chr3g0143991	79.221	77	16	0	46	122	40	116	3.00e-36	123
Msa1063130	MtrunA17_Chr8g0370431	56.349	126	47	3	16	141	9	126	5.13e-35	119
Msa1063130	MtrunA17_Chr5g0407011	54.286	140	48	5	16	154	9	133	3.67e-32	112
Msa1063130	MtrunA17_Chr1g0212341	50.000	114	53	2	47	157	98	210	4.34e-27	101
Msa1063130	MtrunA17_Chr4g0052011	48.315	89	46	0	38	126	51	139	6.67e-22	86.3
Msa1063130	MtrunA17_Chr1g0155251	43.820	89	48	2	47	134	554	641	2.46e-12	63.9
Msa1063130	MtrunA17_Chr8g0387931	43.421	76	40	1	47	122	262	334	2.48e-11	61.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063130	AT3G51880.2	67.081	161	50	1	3	163	5	162	1.28e-63	194
Msa1063130	AT3G51880.5	70.548	146	40	1	3	148	5	147	4.19e-63	192
Msa1063130	AT3G51880.1	70.548	146	40	1	3	148	5	147	4.19e-63	192
Msa1063130	AT3G51880.3	70.548	146	40	1	3	148	5	147	4.19e-63	192
Msa1063130	AT3G51880.4	69.784	139	39	1	3	141	5	140	7.54e-59	181
Msa1063130	AT1G20693.2	63.866	119	37	1	42	160	29	141	2.65e-41	135
Msa1063130	AT1G20696.1	62.903	124	42	1	22	141	2	125	6.61e-40	132
Msa1063130	AT1G20693.3	68.000	100	32	0	42	141	29	128	2.81e-38	128
Msa1063130	AT1G20693.1	68.000	100	32	0	42	141	29	128	2.90e-38	128
Msa1063130	AT2G17560.2	60.000	115	42	2	42	155	26	137	9.69e-37	124

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Find 64 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTCCATATGAAGCCA+AGG	0.600500	7_2:+54681586	Msa1063130:CDS
AAAGACTAAGAAGGAGAAGA+AGG	0.533667	7_2:+54680969	Msa1063130:CDS
AAGACGAAGAAAGCGCTGGG+AGG	0.712358	7_2:-54681021	None:intergenic
AAGACGATGACAAAGGGCAA+GGG	0.619855	7_2:+54679801	Msa1063130:exon
AAGTTCAGCGGACGATGACG+AGG	0.620749	7_2:+54681750	Msa1063130:CDS
AATAATGAAGATGAAGCAAG+TGG	0.555668	7_2:+54681802	Msa1063130:CDS
AATGTTTCATTGGTGGAGGG+AGG	0.535007	7_2:+54682261	Msa1063130:three_prime_UTR
ACAACTGAGGGAAGTTCAAG+TGG	0.622358	7_2:-54682372	None:intergenic
ACTTTGATTGTAGGTTGGAA+AGG	0.560820	7_2:+54681261	Msa1063130:intron
AGAACGAAGACGATGACAAA+GGG	0.510462	7_2:+54679795	Msa1063130:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCACCTTAAAAATGGATGAA+GGG	-	chr7_2:54679085-54679104	None:intergenic	30.0%
AAAAAGGGTCACCTTAAAAA+TGG	-	chr7_2:54679093-54679112	None:intergenic	30.0%
CAGAGGAAAAAAGAGAAAAA+GGG	-	chr7_2:54679108-54679127	None:intergenic	30.0%
ATGATGTTTGATGAGTTATG+AGG	+	chr7_2:54679216-54679235	Msa1063130:intron	30.0%
TTGGTCAAATATCTGAATTG+AGG	+	chr7_2:54679375-54679394	Msa1063130:intron	30.0%
TTTAAATCACAGTCATGGTT+TGG	+	chr7_2:54679554-54679573	Msa1063130:intron	30.0%
CCAACTGTCCAAAAAAATAT+AGG	-	chr7_2:54680044-54680063	None:intergenic	30.0%
TTGCTCAAATATTACTCTCT+AGG	-	chr7_2:54680717-54680736	None:intergenic	30.0%
ACAAATATTAATTGGTGCAG+TGG	-	chr7_2:54680746-54680765	None:intergenic	30.0%
ATGTAAACTTACAGCTGATA+CGG	-	chr7_2:54681176-54681195	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	54678997	54682559	54678997	ID=Msa1063130;Name=Msa1063130
chr7_2	mRNA	54678997	54682559	54678997	ID=Msa1063130-mRNA-1;Parent=Msa1063130;Name=Msa1063130-mRNA-1;_AED=0.07;_eAED=0.07;_QI=130\|1\|1\|1\|0.85\|0.75\|8\|324\|172
chr7_2	exon	54678997	54679100	54678997	ID=Msa1063130-mRNA-1:exon:10030;Parent=Msa1063130-mRNA-1
chr7_2	exon	54679781	54679868	54679781	ID=Msa1063130-mRNA-1:exon:10031;Parent=Msa1063130-mRNA-1
chr7_2	exon	54680924	54681046	54680924	ID=Msa1063130-mRNA-1:exon:10032;Parent=Msa1063130-mRNA-1
chr7_2	exon	54681126	54681183	54681126	ID=Msa1063130-mRNA-1:exon:10033;Parent=Msa1063130-mRNA-1
chr7_2	exon	54681274	54681318	54681274	ID=Msa1063130-mRNA-1:exon:10034;Parent=Msa1063130-mRNA-1
chr7_2	exon	54681584	54681661	54681584	ID=Msa1063130-mRNA-1:exon:10035;Parent=Msa1063130-mRNA-1
chr7_2	exon	54681748	54681828	54681748	ID=Msa1063130-mRNA-1:exon:10036;Parent=Msa1063130-mRNA-1
chr7_2	exon	54682164	54682559	54682164	ID=Msa1063130-mRNA-1:exon:10037;Parent=Msa1063130-mRNA-1
chr7_2	five_prime_UTR	54678997	54679100	54678997	ID=Msa1063130-mRNA-1:five_prime_utr;Parent=Msa1063130-mRNA-1
chr7_2	five_prime_UTR	54679781	54679806	54679781	ID=Msa1063130-mRNA-1:five_prime_utr;Parent=Msa1063130-mRNA-1
chr7_2	CDS	54679807	54679868	54679807	ID=Msa1063130-mRNA-1:cds;Parent=Msa1063130-mRNA-1
chr7_2	CDS	54680924	54681046	54680924	ID=Msa1063130-mRNA-1:cds;Parent=Msa1063130-mRNA-1
chr7_2	CDS	54681126	54681183	54681126	ID=Msa1063130-mRNA-1:cds;Parent=Msa1063130-mRNA-1
chr7_2	CDS	54681274	54681318	54681274	ID=Msa1063130-mRNA-1:cds;Parent=Msa1063130-mRNA-1
chr7_2	CDS	54681584	54681661	54681584	ID=Msa1063130-mRNA-1:cds;Parent=Msa1063130-mRNA-1
chr7_2	CDS	54681748	54681828	54681748	ID=Msa1063130-mRNA-1:cds;Parent=Msa1063130-mRNA-1
chr7_2	CDS	54682164	54682235	54682164	ID=Msa1063130-mRNA-1:cds;Parent=Msa1063130-mRNA-1
chr7_2	three_prime_UTR	54682236	54682559	54682236	ID=Msa1063130-mRNA-1:three_prime_utr;Parent=Msa1063130-mRNA-1

Gene Sequence

>Msa1063130

ATGACAAAGGGCAAGGGAACAGCAAAGACTTCAAGGGATGTTTTGAAACCAGTTGATGACCGAAAAGTAGGTAAGAGAAAGGCTGCTGCTAAACCTGAGAAGGTGCCAAAGACTAAGAAGGAGAAGAAGGCCAAGAAAGATCCCAACAAACCAAAAAGACCTCCCAGCGCTTTCTTCGTCTTCCTTGAGGATTTCAGAAAGACTTTCAAGGCAGAGAACCCTAATGTGAAGGCCGTATCAGCTGTTGGAAAGGCTGGAGGAGAGAAATGGAAATCCTTGACTAAGGCTGAAAAAGCTCCATATGAAGCCAAGGCTGCAAAAAGGAAAGTTGAGTATGAAAAGCTTATGAATGCTTATAATAACAAGCCAAGTTCAGCGGACGATGACGAGGAGGAATCAGACAAAGACAAATCTGAAGTTAATAATGAAGATGAAGCAAGTGGAGAGGATGACCACCAAGATGATGAGGAAGAGGACGACGAGGACGAAGATGACGATGACGATGAAGACGATGAGTGA

Protein sequence

>Msa1063130

MTKGKGTAKTSRDVLKPVDDRKVGKRKAAAKPEKVPKTKKEKKAKKDPNKPKRPPSAFFVFLEDFRKTFKAENPNVKAVSAVGKAGGEKWKSLTKAEKAPYEAKAAKRKVEYEKLMNAYNNKPSSADDDEEESDKDKSEVNNEDEASGEDDHQDDEEEDDEDEDDDDDEDDE*