AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1063680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1063680 MtrunA17_Chr7g0243081 92.655 354 20 2 1 350 1 352 0.0 641
Msa1063680 MtrunA17_Chr8g0379121 58.824 119 42 2 21 132 58 176 6.51e-38 140
Msa1063680 MtrunA17_Chr2g0320231 52.288 153 51 3 2 132 10 162 1.53e-37 138
Msa1063680 MtrunA17_Chr2g0288601 76.471 68 16 0 51 118 55 122 6.28e-32 116
Msa1063680 MtrunA17_Chr2g0283951 68.605 86 23 1 50 131 72 157 9.12e-32 122
Msa1063680 MtrunA17_Chr1g0210911 72.727 66 18 0 58 123 13 78 2.14e-28 112
Msa1063680 MtrunA17_Chr1g0162931 65.278 72 25 0 52 123 3 74 4.06e-28 110
Msa1063680 MtrunA17_Chr6g0479431 55.294 85 31 1 66 150 201 278 3.72e-23 99.0
Msa1063680 MtrunA17_Chr8g0366281 44.144 111 58 1 35 141 137 247 1.50e-22 97.1
Msa1063680 MtrunA17_Chr1g0154261 59.211 76 29 1 66 141 125 198 1.99e-22 95.5
Msa1063680 MtrunA17_Chr5g0410741 56.098 82 35 1 61 141 212 293 4.90e-22 96.3
Msa1063680 MtrunA17_Chr3g0095611 42.105 114 56 3 49 162 1 104 7.04e-22 92.4
Msa1063680 MtrunA17_Chr3g0109981 64.179 67 23 1 61 126 267 333 2.26e-21 94.7
Msa1063680 MtrunA17_Chr7g0261911 64.286 56 20 0 68 123 263 318 5.53e-19 87.8
Msa1063680 MtrunA17_Chr6g0461641 66.038 53 18 0 68 120 43 95 1.72e-18 85.9
Msa1063680 MtrunA17_Chr1g0190301 62.500 56 21 0 68 123 176 231 1.85e-18 85.9
Msa1063680 MtrunA17_Chr7g0274471 62.500 56 21 0 68 123 219 274 2.41e-18 85.9
Msa1063680 MtrunA17_Chr2g0321461 62.500 56 21 0 68 123 46 101 2.57e-18 85.1
Msa1063680 MtrunA17_Chr5g0409731 62.069 58 22 0 63 120 21 78 3.63e-18 83.6
Msa1063680 MtrunA17_Chr7g0258551 49.367 79 39 1 63 140 208 286 3.66e-18 84.7
Msa1063680 MtrunA17_Chr7g0254981 55.224 67 30 0 59 125 42 108 1.06e-17 82.0
Msa1063680 MtrunA17_Chr1g0197171 60.714 56 22 0 68 123 45 100 6.16e-17 80.1
Msa1063680 MtrunA17_Chr2g0290991 60.714 56 22 0 68 123 40 95 7.94e-17 80.1
Msa1063680 MtrunA17_Chr5g0417521 55.357 56 25 0 68 123 16 71 1.53e-16 79.7
Msa1063680 MtrunA17_Chr2g0291021 61.404 57 22 0 69 125 51 107 1.80e-16 79.3
Msa1063680 MtrunA17_Chr1g0175091 52.055 73 34 1 69 140 260 332 2.53e-16 79.7
Msa1063680 MtrunA17_Chr1g0175081 56.364 55 24 0 69 123 24 78 3.39e-16 73.6
Msa1063680 MtrunA17_Chr8g0356501 55.357 56 25 0 68 123 38 93 1.21e-15 77.0
Msa1063680 MtrunA17_Chr2g0310011 55.357 56 25 0 68 123 36 91 3.15e-15 75.9
Msa1063680 MtrunA17_Chr4g0042451 55.357 56 25 0 68 123 18 73 4.13e-15 75.5
Msa1063680 MtrunA17_Chr7g0242521 56.667 60 23 1 69 125 15 74 2.87e-13 68.6
Msa1063680 MtrunA17_Chr4g0009901 51.786 56 26 1 65 120 166 220 1.13e-12 67.4
Msa1063680 MtrunA17_Chr1g0175071 54.717 53 24 0 69 121 179 231 1.36e-12 67.8
Msa1063680 MtrunA17_Chr4g0045361 45.205 73 31 1 57 120 255 327 2.86e-12 67.4
Msa1063680 MtrunA17_Chr4g0031961 48.684 76 37 2 46 120 109 183 6.50e-12 65.9
Msa1063680 MtrunA17_Chr2g0304841 39.316 117 57 6 66 178 212 318 8.09e-12 66.6
Msa1063680 MtrunA17_Chr4g0062921 55.769 52 23 0 69 120 237 288 1.05e-11 65.9
Msa1063680 MtrunA17_Chr7g0258531 49.231 65 33 0 69 133 215 279 1.81e-11 64.7
Msa1063680 MtrunA17_Chr7g0275401 52.632 57 26 1 66 122 191 246 3.67e-11 63.2
Msa1063680 MtrunA17_Chr5g0422621 55.102 49 22 0 72 120 203 251 7.44e-11 62.8
Msa1063680 MtrunA17_Chr1g0198601 55.102 49 22 0 72 120 216 264 8.59e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1063680 AT2G38300.1 42.547 322 157 14 44 350 32 340 9.81e-57 188
Msa1063680 AT2G40260.1 63.889 108 37 1 50 157 65 170 5.23e-39 143
Msa1063680 AT2G42660.1 78.788 66 14 0 59 124 42 107 2.44e-31 119
Msa1063680 AT2G02060.2 65.432 81 28 0 40 120 2 82 1.71e-29 114
Msa1063680 AT2G02060.1 65.432 81 28 0 40 120 2 82 1.71e-29 114
Msa1063680 AT4G04605.1 59.302 86 33 1 55 138 2 87 1.68e-28 110
Msa1063680 AT1G14600.1 68.657 67 21 0 59 125 15 81 2.40e-28 111
Msa1063680 AT4G04580.1 58.209 67 28 0 57 123 4 70 3.93e-23 94.7
Msa1063680 AT5G42630.1 67.213 61 20 0 66 126 104 164 2.37e-22 95.5
Msa1063680 AT5G42630.2 67.213 61 20 0 66 126 104 164 2.42e-22 94.4
Msa1063680 AT1G32240.1 54.878 82 36 1 61 141 206 287 1.02e-21 95.5
Msa1063680 AT1G32240.2 54.878 82 36 1 61 141 206 287 1.11e-21 95.1
Msa1063680 AT4G04555.1 60.000 65 26 0 59 123 6 70 1.93e-21 88.2
Msa1063680 AT5G16560.1 58.108 74 30 1 61 133 212 285 6.45e-21 93.2
Msa1063680 AT4G17695.1 62.295 61 23 0 66 126 163 223 4.15e-20 90.1
Msa1063680 AT5G45580.1 62.069 58 22 0 63 120 3 60 2.25e-18 84.0
Msa1063680 AT5G45580.2 62.069 58 22 0 63 120 19 76 2.53e-18 84.3
Msa1063680 AT3G04030.1 58.929 56 23 0 68 123 46 101 1.95e-17 83.2
Msa1063680 AT3G04030.3 58.929 56 23 0 68 123 46 101 2.22e-17 83.2
Msa1063680 AT3G04450.3 49.333 75 30 1 57 123 220 294 2.40e-17 82.0
Msa1063680 AT3G04450.4 49.333 75 30 1 57 123 180 254 2.56e-17 82.8
Msa1063680 AT3G04450.2 49.333 75 30 1 57 123 180 254 2.56e-17 82.8
Msa1063680 AT3G04450.1 49.333 75 30 1 57 123 220 294 2.70e-17 83.2
Msa1063680 AT5G29000.1 63.636 55 20 0 69 123 190 244 3.59e-17 82.0
Msa1063680 AT5G29000.3 63.636 55 20 0 69 123 190 244 3.59e-17 82.0
Msa1063680 AT4G28610.1 61.818 55 21 0 69 123 227 281 3.60e-17 82.4
Msa1063680 AT5G18240.2 58.929 56 23 0 68 123 46 101 3.67e-17 82.4
Msa1063680 AT5G18240.3 58.929 56 23 0 68 123 46 101 3.67e-17 82.4
Msa1063680 AT5G29000.4 63.636 55 20 0 69 123 233 287 4.02e-17 81.3
Msa1063680 AT5G29000.2 63.636 55 20 0 69 123 233 287 4.39e-17 82.4
Msa1063680 AT5G18240.5 58.929 56 23 0 68 123 46 101 4.45e-17 82.0
Msa1063680 AT5G18240.1 58.929 56 23 0 68 123 46 101 4.54e-17 82.0
Msa1063680 AT5G18240.4 58.929 56 23 0 68 123 46 101 4.54e-17 82.0
Msa1063680 AT2G01060.1 61.404 57 22 0 69 125 17 73 1.02e-16 79.7
Msa1063680 AT3G12730.1 57.143 56 24 0 68 123 24 79 1.54e-16 78.6
Msa1063680 AT4G13640.1 56.604 53 23 0 68 120 38 90 5.85e-16 77.8
Msa1063680 AT4G13640.2 56.604 53 23 0 68 120 38 90 6.11e-16 77.8
Msa1063680 AT3G24120.1 56.604 53 23 0 68 120 42 94 7.06e-16 77.4
Msa1063680 AT3G13040.3 59.649 57 23 0 69 125 243 299 7.49e-16 79.0
Msa1063680 AT3G13040.1 59.649 57 23 0 69 125 243 299 7.49e-16 79.0
Msa1063680 AT3G13040.2 59.649 57 23 0 69 125 243 299 7.49e-16 79.0
Msa1063680 AT3G24120.2 56.604 53 23 0 68 120 42 94 7.88e-16 77.4
Msa1063680 AT2G20400.2 57.895 57 24 0 69 125 233 289 2.00e-15 77.4
Msa1063680 AT1G79430.2 55.357 56 25 0 68 123 35 90 2.02e-15 77.0
Msa1063680 AT2G20400.1 57.895 57 24 0 69 125 233 289 2.03e-15 77.0
Msa1063680 AT5G06800.3 54.098 61 28 0 69 129 194 254 3.42e-15 75.5
Msa1063680 AT3G04030.2 57.143 56 23 1 68 123 46 100 6.17e-15 75.9
Msa1063680 AT1G69580.1 55.357 56 25 0 68 123 35 90 7.39e-15 75.1
Msa1063680 AT1G69580.2 55.357 56 25 0 68 123 35 90 7.80e-15 75.1
Msa1063680 AT5G06800.2 54.098 61 28 0 69 129 194 254 8.08e-15 75.5
Msa1063680 AT5G06800.1 54.098 61 28 0 69 129 194 254 8.63e-15 75.1
Msa1063680 AT2G06020.1 35.652 115 63 3 15 120 27 139 1.02e-13 71.2
Msa1063680 AT5G59570.2 45.455 88 44 3 35 120 108 193 3.32e-13 69.7
Msa1063680 AT5G59570.1 45.455 88 44 3 35 120 108 193 3.32e-13 69.7
Msa1063680 AT2G40260.2 58.929 56 21 1 102 157 1 54 2.09e-12 67.4
Msa1063680 AT1G25550.1 59.184 49 20 0 72 120 214 262 2.39e-12 67.8
Msa1063680 AT2G03500.1 57.143 49 21 0 72 120 238 286 1.23e-11 65.9
Msa1063680 AT1G25550.2 46.269 67 32 1 72 138 214 276 1.52e-11 64.7
Msa1063680 AT3G46640.2 54.545 55 24 1 66 120 143 196 2.02e-11 64.7
Msa1063680 AT3G46640.1 54.545 55 24 1 66 120 143 196 2.02e-11 64.7
Msa1063680 AT3G46640.3 54.545 55 24 1 66 120 143 196 3.10e-11 64.3
Msa1063680 AT3G62670.1 43.056 72 34 2 53 120 200 268 3.40e-11 64.7

Find 72 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTGAAGAACCAATTT+TGG 0.129269 7_2:-55775688 None:intergenic
TGTTGATAGTTCTCTAAAAT+TGG 0.214021 7_2:-55776768 None:intergenic
TCTATATATATGTCAATTAT+TGG 0.224109 7_2:-55775061 None:intergenic
CTTCAATTGATGAATATAAA+AGG 0.228877 7_2:+55775700 Msa1063680:CDS
GATATTGATGTTGCTATTAT+TGG 0.260085 7_2:-55776714 None:intergenic
GAAAGGAGTCAATATTTCTT+TGG 0.270906 7_2:+55777126 Msa1063680:CDS
AAAGATTCATGGCCACAAAA+TGG 0.279832 7_2:-55776824 None:intergenic
AAATATTTCATTTCATCATT+TGG 0.298942 7_2:+55775025 Msa1063680:five_prime_UTR
CTATATGATGATGTTTGATT+TGG 0.300830 7_2:-55776109 None:intergenic
GGAAATTATCATTTGGTTCA+TGG 0.322872 7_2:-55776799 None:intergenic
GAAAAGAAAAGCTTCAAATA+TGG 0.323316 7_2:+55776937 Msa1063680:CDS
TCATGTGAAGAGTCATTTAC+AGG 0.340032 7_2:+55775735 Msa1063680:CDS
GATCATCTCTTAAAGATTCA+TGG 0.343857 7_2:-55776835 None:intergenic
TGTAGGAGCAACTCCAAAAT+TGG 0.369328 7_2:+55775675 Msa1063680:intron
TAGAATATGTCTCATTCATA+TGG 0.376004 7_2:-55777155 None:intergenic
GAGGTATATAGATATATTTA+AGG 0.384760 7_2:+55774973 Msa1063680:five_prime_UTR
TCTCATATTCATCTAGCTAT+AGG 0.386781 7_2:-55775119 None:intergenic
AGAAGAACAAGATAGTTCTA+AGG 0.388489 7_2:+55777096 Msa1063680:CDS
GCCTTGAAGCATGGGAAGTT+GGG 0.390047 7_2:-55776080 None:intergenic
ATTAATTGAAACACTTGCCT+TGG 0.400061 7_2:-55775170 None:intergenic
AATGGGAGGAAATTATCATT+TGG 0.400885 7_2:-55776806 None:intergenic
CTCGAAGGAGGGTGAACAAT+AGG 0.402435 7_2:+55774949 Msa1063680:five_prime_UTR
TATCACTAAAATTAAATTCA+AGG 0.404007 7_2:+55776975 Msa1063680:CDS
TAAAATTGGATGAATCCATT+TGG 0.405764 7_2:-55776754 None:intergenic
CTTCATGCTGTGCAAAGACT+TGG 0.407098 7_2:+55775370 Msa1063680:CDS
ACCCAACTTCCCATGCTTCA+AGG 0.422458 7_2:+55776079 Msa1063680:CDS
GAATCTAGAGCTGAGTTCTA+TGG 0.429313 7_2:+55776674 Msa1063680:CDS
AAGATTCATGGCCACAAAAT+GGG 0.429721 7_2:-55776823 None:intergenic
CAAATCAAACATCATCATAT+AGG 0.434083 7_2:+55776110 Msa1063680:CDS
TTTACAGATGTGGATCATAT+GGG 0.447788 7_2:+55776600 Msa1063680:intron
AATCATCTTATGTTTGCAAA+AGG 0.448039 7_2:+55777068 Msa1063680:CDS
TAACAGTGTTGGCTGATCAT+AGG 0.450325 7_2:+55776017 Msa1063680:intron
TAATAGCAACATCAATATCC+AGG 0.451694 7_2:+55776718 Msa1063680:CDS
GAAATGAGATCTTGTGCTCT+TGG 0.453092 7_2:-55776869 None:intergenic
AGAAAACGAAAATGAAGATC+AGG 0.462194 7_2:-55775349 None:intergenic
CCAACGTAGCCTAGGCAACT+TGG 0.470061 7_2:-55775323 None:intergenic
AGCCTTGAAGCATGGGAAGT+TGG 0.470813 7_2:-55776081 None:intergenic
GGAGTATAGCCTTGAAGCAT+GGG 0.488714 7_2:-55776088 None:intergenic
ATGAGAGAGGAAGAAACATA+TGG 0.490280 7_2:+55775136 Msa1063680:CDS
TTCAAATATGGATCTTGAGT+TGG 0.495575 7_2:+55776949 Msa1063680:CDS
TGGAGTATAGCCTTGAAGCA+TGG 0.512500 7_2:-55776089 None:intergenic
GGCAACTTGGATCTAACATA+AGG 0.518664 7_2:-55775310 None:intergenic
ATGTTAGATCCAAGTTGCCT+AGG 0.523365 7_2:+55775314 Msa1063680:CDS
AGTTCTAAGGAGCAAGAGAA+AGG 0.525178 7_2:+55777109 Msa1063680:CDS
CATGCTGTGCAAAGACTTGG+TGG 0.535016 7_2:+55775373 Msa1063680:CDS
GTGTAGAAGCTTGAAAGTTG+TGG 0.545562 7_2:+55775094 Msa1063680:five_prime_UTR
AAAGTAGTTGACACAAATCA+AGG 0.545661 7_2:+55775891 Msa1063680:CDS
TTTGGAAAATACTATGACCC+TGG 0.552012 7_2:-55776736 None:intergenic
AGAGATTTCTAAATTCTATG+TGG 0.554216 7_2:+55774913 None:intergenic
TCAGGAGTCCAACGTAGCCT+AGG 0.559435 7_2:-55775331 None:intergenic
CCAAGTTGCCTAGGCTACGT+TGG 0.561524 7_2:+55775323 Msa1063680:CDS
TCTTGCCATGTATGAAAACA+TGG 0.579727 7_2:+55776631 Msa1063680:CDS
TTGACATATATATAGATCAA+AGG 0.580822 7_2:+55775068 Msa1063680:five_prime_UTR
CATAGGCTTCTTGTAGACAA+TGG 0.581433 7_2:+55776034 Msa1063680:CDS
CTAACATAAGGCCTCACTGA+TGG 0.585574 7_2:-55775298 None:intergenic
TTACAGATGTGGATCATATG+GGG 0.594097 7_2:+55776601 Msa1063680:intron
TATGCTAAATTACTCGAAGG+AGG 0.598837 7_2:+55774937 Msa1063680:five_prime_UTR
ATATATATGTCAATTATTGG+TGG 0.604611 7_2:-55775058 None:intergenic
AAATTAAATTCAAGGAGCAA+TGG 0.605129 7_2:+55776983 Msa1063680:CDS
TAACTTGCCTGAAAATCAAG+AGG 0.607982 7_2:+55776898 Msa1063680:CDS
GATGTGCAAAAGAACAACAA+TGG 0.621747 7_2:+55775226 Msa1063680:CDS
AATAGCAACATCAATATCCA+GGG 0.624382 7_2:+55776719 Msa1063680:CDS
TGGTGATCATAAATGCATAG+AGG 0.634446 7_2:+55777003 Msa1063680:CDS
GATGATTGTGATATTAGTGA+AGG 0.639297 7_2:+55775196 Msa1063680:CDS
ATATGGATCAGAATGTTCCA+AGG 0.643313 7_2:+55775153 Msa1063680:CDS
ATGCTAAATTACTCGAAGGA+GGG 0.643688 7_2:+55774938 Msa1063680:five_prime_UTR
GTGCAAAAGAACAACAATGG+AGG 0.658548 7_2:+55775229 Msa1063680:CDS
TAGCTAGATGAATATGAGAG+AGG 0.659511 7_2:+55775123 Msa1063680:exon
GGATATGCTAAATTACTCGA+AGG 0.659879 7_2:+55774934 Msa1063680:five_prime_UTR
ATTCATGGCCACAAAATGGG+AGG 0.669541 7_2:-55776820 None:intergenic
AGGAGGGTGAACAATAGGAG+AGG 0.694351 7_2:+55774954 Msa1063680:five_prime_UTR
GTGAGAAGAAACCATCAGTG+AGG 0.735513 7_2:+55775287 Msa1063680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTAATTTTATTTTT+TGG - chr7_2:55776412-55776431 None:intergenic 0.0%
!! AAATATTTCATTTCATCATT+TGG + chr7_2:55775025-55775044 Msa1063680:five_prime_UTR 15.0%
!! TCTATATATATGTCAATTAT+TGG - chr7_2:55775064-55775083 None:intergenic 15.0%
!! TAAAAAGTCATAGATAAATA+TGG + chr7_2:55775758-55775777 Msa1063680:intron 15.0%
!! AATAAGTGATTACATAATTA+GGG + chr7_2:55775952-55775971 Msa1063680:intron 15.0%
!!! TGAATTAACCATTTTTTTTA+TGG + chr7_2:55776148-55776167 Msa1063680:intron 15.0%
!!! TTTTTTTTTTTTTACAGATG+TGG + chr7_2:55776590-55776609 Msa1063680:intron 15.0%
!! TATCACTAAAATTAAATTCA+AGG + chr7_2:55776975-55776994 Msa1063680:CDS 15.0%
!! GAGGTATATAGATATATTTA+AGG + chr7_2:55774973-55774992 Msa1063680:five_prime_UTR 20.0%
!! ATATATATGTCAATTATTGG+TGG - chr7_2:55775061-55775080 None:intergenic 20.0%
!! TTGACATATATATAGATCAA+AGG + chr7_2:55775068-55775087 Msa1063680:five_prime_UTR 20.0%
!! GAAAAATTGAAATCTAGAAA+AGG - chr7_2:55775537-55775556 None:intergenic 20.0%
!!! TCTAGATTTCAATTTTTCAT+AGG + chr7_2:55775539-55775558 Msa1063680:intron 20.0%
!!! CTAGATTTCAATTTTTCATA+GGG + chr7_2:55775540-55775559 Msa1063680:intron 20.0%
!!! TAGATTTCAATTTTTCATAG+GGG + chr7_2:55775541-55775560 Msa1063680:intron 20.0%
!! CTTCAATTGATGAATATAAA+AGG + chr7_2:55775700-55775719 Msa1063680:CDS 20.0%
!! CAATAAGTGATTACATAATT+AGG + chr7_2:55775951-55775970 Msa1063680:intron 20.0%
!!! AAGACCTTTTTTTATTCATA+GGG + chr7_2:55776300-55776319 Msa1063680:intron 20.0%
!! AGTTATCAAAACAAATACAA+AGG + chr7_2:55776359-55776378 Msa1063680:intron 20.0%
!! GTTATCAAAACAAATACAAA+GGG + chr7_2:55776360-55776379 Msa1063680:intron 20.0%
!!! TTTTATTTTTTGGTCAAGTT+CGG - chr7_2:55776402-55776421 None:intergenic 20.0%
!!! TTTTAAACGTAAATGACTTA+AGG - chr7_2:55776487-55776506 None:intergenic 20.0%
!! TATTATTGTCTTAGAATTGA+AGG + chr7_2:55776560-55776579 Msa1063680:intron 20.0%
! TTACATATATGTTATGTGTG+CGG - chr7_2:55775414-55775433 None:intergenic 25.0%
!! TTAATGATTTTCTTGTGTGT+AGG + chr7_2:55775658-55775677 Msa1063680:intron 25.0%
!! ATCAATTGAAGAACCAATTT+TGG - chr7_2:55775691-55775710 None:intergenic 25.0%
! TTGTATATATGTAACAGTGT+TGG + chr7_2:55776006-55776025 Msa1063680:intron 25.0%
!! CTATATGATGATGTTTGATT+TGG - chr7_2:55776112-55776131 None:intergenic 25.0%
! CAAATCAAACATCATCATAT+AGG + chr7_2:55776110-55776129 Msa1063680:CDS 25.0%
! AGCATTCTCCATAAAAAAAA+TGG - chr7_2:55776159-55776178 None:intergenic 25.0%
!!! CAAGACCTTTTTTTATTCAT+AGG + chr7_2:55776299-55776318 Msa1063680:intron 25.0%
! TGATCCCTATGAATAAAAAA+AGG - chr7_2:55776307-55776326 None:intergenic 25.0%
! GTAAATGACTTAAGGAAATT+TGG - chr7_2:55776479-55776498 None:intergenic 25.0%
!! GATATTGATGTTGCTATTAT+TGG - chr7_2:55776717-55776736 None:intergenic 25.0%
! TAAAATTGGATGAATCCATT+TGG - chr7_2:55776757-55776776 None:intergenic 25.0%
!!! TGTTGATAGTTCTCTAAAAT+TGG - chr7_2:55776771-55776790 None:intergenic 25.0%
! GAAAAGAAAAGCTTCAAATA+TGG + chr7_2:55776937-55776956 Msa1063680:CDS 25.0%
! AAATTAAATTCAAGGAGCAA+TGG + chr7_2:55776983-55777002 Msa1063680:CDS 25.0%
! AATCATCTTATGTTTGCAAA+AGG + chr7_2:55777068-55777087 Msa1063680:CDS 25.0%
TCTCATATTCATCTAGCTAT+AGG - chr7_2:55775122-55775141 None:intergenic 30.0%
ATTAATTGAAACACTTGCCT+TGG - chr7_2:55775173-55775192 None:intergenic 30.0%
GATGATTGTGATATTAGTGA+AGG + chr7_2:55775196-55775215 Msa1063680:CDS 30.0%
AGAAAACGAAAATGAAGATC+AGG - chr7_2:55775352-55775371 None:intergenic 30.0%
GTAACCTACAATTTACAACA+TGG + chr7_2:55775468-55775487 Msa1063680:intron 30.0%
ATCTATGTGTTTGATCATCA+TGG - chr7_2:55775500-55775519 None:intergenic 30.0%
TTCAATATCAAGTTAGTCCT+AGG + chr7_2:55775566-55775585 Msa1063680:intron 30.0%
TTGAATTGATTAACCTACCT+AGG - chr7_2:55775586-55775605 None:intergenic 30.0%
AAAGCTAGCTAGCTATATAT+AGG - chr7_2:55775790-55775809 None:intergenic 30.0%
AAAGTAGTTGACACAAATCA+AGG + chr7_2:55775891-55775910 Msa1063680:CDS 30.0%
ACACAAATCAAGGTACAATA+AGG + chr7_2:55775901-55775920 Msa1063680:intron 30.0%
TTCTTATCGAAAAACGAGAA+GGG - chr7_2:55776336-55776355 None:intergenic 30.0%
ATTCTTATCGAAAAACGAGA+AGG - chr7_2:55776337-55776356 None:intergenic 30.0%
! AAGGAAATTTGGAACAAAGT+TGG - chr7_2:55776468-55776487 None:intergenic 30.0%
! CGTTTAAAATTGTAACCTCA+AGG + chr7_2:55776498-55776517 Msa1063680:intron 30.0%
! GATTTGAAGTTTAAACCTTG+AGG - chr7_2:55776516-55776535 None:intergenic 30.0%
! AGTAATTTTACAACTGTCGT+TGG - chr7_2:55776541-55776560 None:intergenic 30.0%
! TTTTACAGATGTGGATCATA+TGG + chr7_2:55776599-55776618 Msa1063680:intron 30.0%
TTTACAGATGTGGATCATAT+GGG + chr7_2:55776600-55776619 Msa1063680:intron 30.0%
! TTGTACCATGTTTTCATACA+TGG - chr7_2:55776639-55776658 None:intergenic 30.0%
TAATAGCAACATCAATATCC+AGG + chr7_2:55776718-55776737 Msa1063680:CDS 30.0%
AATAGCAACATCAATATCCA+GGG + chr7_2:55776719-55776738 Msa1063680:CDS 30.0%
! GGAAATTATCATTTGGTTCA+TGG - chr7_2:55776802-55776821 None:intergenic 30.0%
AATGGGAGGAAATTATCATT+TGG - chr7_2:55776809-55776828 None:intergenic 30.0%
GATCATCTCTTAAAGATTCA+TGG - chr7_2:55776838-55776857 None:intergenic 30.0%
TTCAAATATGGATCTTGAGT+TGG + chr7_2:55776949-55776968 Msa1063680:CDS 30.0%
! AGAAGAACAAGATAGTTCTA+AGG + chr7_2:55777096-55777115 Msa1063680:CDS 30.0%
GAAAGGAGTCAATATTTCTT+TGG + chr7_2:55777126-55777145 Msa1063680:CDS 30.0%
GGATATGCTAAATTACTCGA+AGG + chr7_2:55774934-55774953 Msa1063680:five_prime_UTR 35.0%
TATGCTAAATTACTCGAAGG+AGG + chr7_2:55774937-55774956 Msa1063680:five_prime_UTR 35.0%
ATGCTAAATTACTCGAAGGA+GGG + chr7_2:55774938-55774957 Msa1063680:five_prime_UTR 35.0%
TAGCTAGATGAATATGAGAG+AGG + chr7_2:55775123-55775142 Msa1063680:exon 35.0%
ATGAGAGAGGAAGAAACATA+TGG + chr7_2:55775136-55775155 Msa1063680:CDS 35.0%
ATATGGATCAGAATGTTCCA+AGG + chr7_2:55775153-55775172 Msa1063680:CDS 35.0%
GATGTGCAAAAGAACAACAA+TGG + chr7_2:55775226-55775245 Msa1063680:CDS 35.0%
CACACCATGTTGTAAATTGT+AGG - chr7_2:55775475-55775494 None:intergenic 35.0%
CTACAATTTACAACATGGTG+TGG + chr7_2:55775473-55775492 Msa1063680:intron 35.0%
ATATCAAGTTAGTCCTAGGT+AGG + chr7_2:55775570-55775589 Msa1063680:intron 35.0%
TCATGTGAAGAGTCATTTAC+AGG + chr7_2:55775735-55775754 Msa1063680:CDS 35.0%
CTTTCTTGCTTCTATACATC+TGG - chr7_2:55775875-55775894 None:intergenic 35.0%
! AATTTTACAACTGTCGTTGG+AGG - chr7_2:55776538-55776557 None:intergenic 35.0%
TTACAGATGTGGATCATATG+GGG + chr7_2:55776601-55776620 Msa1063680:intron 35.0%
TCTTGCCATGTATGAAAACA+TGG + chr7_2:55776631-55776650 Msa1063680:CDS 35.0%
TTTGGAAAATACTATGACCC+TGG - chr7_2:55776739-55776758 None:intergenic 35.0%
! GGGTCATAGTATTTTCCAAA+TGG + chr7_2:55776739-55776758 Msa1063680:CDS 35.0%
! GATAATTTCCTCCCATTTTG+TGG + chr7_2:55776812-55776831 Msa1063680:CDS 35.0%
AAGATTCATGGCCACAAAAT+GGG - chr7_2:55776826-55776845 None:intergenic 35.0%
AAAGATTCATGGCCACAAAA+TGG - chr7_2:55776827-55776846 None:intergenic 35.0%
! TAACTTGCCTGAAAATCAAG+AGG + chr7_2:55776898-55776917 Msa1063680:CDS 35.0%
! AGTTCTTCCTCTTGATTTTC+AGG - chr7_2:55776908-55776927 None:intergenic 35.0%
TGGTGATCATAAATGCATAG+AGG + chr7_2:55777003-55777022 Msa1063680:CDS 35.0%
GTGTAGAAGCTTGAAAGTTG+TGG + chr7_2:55775094-55775113 Msa1063680:five_prime_UTR 40.0%
GTGCAAAAGAACAACAATGG+AGG + chr7_2:55775229-55775248 Msa1063680:CDS 40.0%
GGCAACTTGGATCTAACATA+AGG - chr7_2:55775313-55775332 None:intergenic 40.0%
ATGTTAGATCCAAGTTGCCT+AGG + chr7_2:55775314-55775333 Msa1063680:CDS 40.0%
TGTAGGAGCAACTCCAAAAT+TGG + chr7_2:55775675-55775694 Msa1063680:intron 40.0%
!! TAACAGTGTTGGCTGATCAT+AGG + chr7_2:55776017-55776036 Msa1063680:intron 40.0%
CATAGGCTTCTTGTAGACAA+TGG + chr7_2:55776034-55776053 Msa1063680:CDS 40.0%
ATCTTTAACCATTACCCCTG+AGG + chr7_2:55776265-55776284 Msa1063680:intron 40.0%
TCTTTAACCATTACCCCTGA+GGG + chr7_2:55776266-55776285 Msa1063680:intron 40.0%
! GAATCTAGAGCTGAGTTCTA+TGG + chr7_2:55776674-55776693 Msa1063680:CDS 40.0%
GAAATGAGATCTTGTGCTCT+TGG - chr7_2:55776872-55776891 None:intergenic 40.0%
!! AGTTCTAAGGAGCAAGAGAA+AGG + chr7_2:55777109-55777128 Msa1063680:CDS 40.0%
GTGAGAAGAAACCATCAGTG+AGG + chr7_2:55775287-55775306 Msa1063680:CDS 45.0%
CTAACATAAGGCCTCACTGA+TGG - chr7_2:55775301-55775320 None:intergenic 45.0%
CTTCATGCTGTGCAAAGACT+TGG + chr7_2:55775370-55775389 Msa1063680:CDS 45.0%
! GGAGTATAGCCTTGAAGCAT+GGG - chr7_2:55776091-55776110 None:intergenic 45.0%
! TGGAGTATAGCCTTGAAGCA+TGG - chr7_2:55776092-55776111 None:intergenic 45.0%
AATGCTAACTAGTGCCCCAA+GGG + chr7_2:55776173-55776192 Msa1063680:intron 45.0%
ATTCATGGCCACAAAATGGG+AGG - chr7_2:55776823-55776842 None:intergenic 45.0%
CTCGAAGGAGGGTGAACAAT+AGG + chr7_2:55774949-55774968 Msa1063680:five_prime_UTR 50.0%
AGGAGGGTGAACAATAGGAG+AGG + chr7_2:55774954-55774973 Msa1063680:five_prime_UTR 50.0%
CATGCTGTGCAAAGACTTGG+TGG + chr7_2:55775373-55775392 Msa1063680:CDS 50.0%
ACCCAACTTCCCATGCTTCA+AGG + chr7_2:55776079-55776098 Msa1063680:CDS 50.0%
GCCTTGAAGCATGGGAAGTT+GGG - chr7_2:55776083-55776102 None:intergenic 50.0%
AGCCTTGAAGCATGGGAAGT+TGG - chr7_2:55776084-55776103 None:intergenic 50.0%
GAATGCTAACTAGTGCCCCA+AGG + chr7_2:55776172-55776191 Msa1063680:intron 50.0%
TTAACCAATGCACGCCCTTG+GGG - chr7_2:55776190-55776209 None:intergenic 50.0%
CTTAACCAATGCACGCCCTT+GGG - chr7_2:55776191-55776210 None:intergenic 50.0%
CCAACGTAGCCTAGGCAACT+TGG - chr7_2:55775326-55775345 None:intergenic 55.0%
CCAAGTTGCCTAGGCTACGT+TGG + chr7_2:55775323-55775342 Msa1063680:CDS 55.0%
TCAGGAGTCCAACGTAGCCT+AGG - chr7_2:55775334-55775353 None:intergenic 55.0%
GCTTAACCAATGCACGCCCT+TGG - chr7_2:55776192-55776211 None:intergenic 55.0%
TTGTTAACCAGCGCCCTCAG+GGG - chr7_2:55776282-55776301 None:intergenic 55.0%
CTTGTTAACCAGCGCCCTCA+GGG - chr7_2:55776283-55776302 None:intergenic 55.0%
TCTTGTTAACCAGCGCCCTC+AGG - chr7_2:55776284-55776303 None:intergenic 55.0%
! AGTGCCCCAAGGGCGTGCAT+TGG + chr7_2:55776183-55776202 Msa1063680:intron 65.0%
! ACCATTACCCCTGAGGGCGC+TGG + chr7_2:55776272-55776291 Msa1063680:intron 65.0%
ACCAGCGCCCTCAGGGGTAA+TGG - chr7_2:55776276-55776295 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 55774921 55777162 55774921 ID=Msa1063680;Name=Msa1063680
chr7_2 mRNA 55774921 55777162 55774921 ID=Msa1063680-mRNA-1;Parent=Msa1063680;Name=Msa1063680-mRNA-1;_AED=0.01;_eAED=0.02;_QI=209|1|0.8|1|1|1|5|0|350
chr7_2 exon 55774921 55775403 55774921 ID=Msa1063680-mRNA-1:exon:10460;Parent=Msa1063680-mRNA-1
chr7_2 exon 55775680 55775756 55775680 ID=Msa1063680-mRNA-1:exon:10461;Parent=Msa1063680-mRNA-1
chr7_2 exon 55775876 55775912 55775876 ID=Msa1063680-mRNA-1:exon:10462;Parent=Msa1063680-mRNA-1
chr7_2 exon 55776023 55776131 55776023 ID=Msa1063680-mRNA-1:exon:10463;Parent=Msa1063680-mRNA-1
chr7_2 exon 55776607 55777162 55776607 ID=Msa1063680-mRNA-1:exon:10464;Parent=Msa1063680-mRNA-1
chr7_2 five_prime_UTR 55774921 55775129 55774921 ID=Msa1063680-mRNA-1:five_prime_utr;Parent=Msa1063680-mRNA-1
chr7_2 CDS 55775130 55775403 55775130 ID=Msa1063680-mRNA-1:cds;Parent=Msa1063680-mRNA-1
chr7_2 CDS 55775680 55775756 55775680 ID=Msa1063680-mRNA-1:cds;Parent=Msa1063680-mRNA-1
chr7_2 CDS 55775876 55775912 55775876 ID=Msa1063680-mRNA-1:cds;Parent=Msa1063680-mRNA-1
chr7_2 CDS 55776023 55776131 55776023 ID=Msa1063680-mRNA-1:cds;Parent=Msa1063680-mRNA-1
chr7_2 CDS 55776607 55777162 55776607 ID=Msa1063680-mRNA-1:cds;Parent=Msa1063680-mRNA-1
Gene Sequence

>Msa1063680

ATGAATATGAGAGAGGAAGAAACATATGGATCAGAATGTTCCAAGGCAAGTGTTTCAATTAATGAAGATGATTGTGATATTAGTGAAGGAAATGAAGATGTGCAAAAGAACAACAATGGAGGAATAAGCTCAAGCAATAGTACTATAGAAGAGAATTGTGAGAAGAAACCATCAGTGAGGCCTTATGTTAGATCCAAGTTGCCTAGGCTACGTTGGACTCCTGATCTTCATTTTCGTTTTCTTCATGCTGTGCAAAGACTTGGTGGACAAGAGAGAGCAACTCCAAAATTGGTTCTTCAATTGATGAATATAAAAGGTTTAAGTATTGCTCATGTGAAGAGTCATTTACAGATGTATAGAAGCAAGAAAGTAGTTGACACAAATCAAGTGTTGGCTGATCATAGGCTTCTTGTAGACAATGGAGACAGAAATGTTTATAATCTCACCCAACTTCCCATGCTTCAAGGCTATACTCCAAATCAAACATCATCATATAGATGTGGATCATATGGGGATGCTTCTCTTGCCATGTATGAAAACATGGTACAAATGAGTTCAATAAGTGAATCTAGAGCTGAGTTCTATGGCAAAATGATTGAGAGAACCAATAATAGCAACATCAATATCCAGGGTCATAGTATTTTCCAAATGGATTCATCCAATTTTAGAGAACTATCAACAACTAAAGTCCATGAACCAAATGATAATTTCCTCCCATTTTGTGGCCATGAATCTTTAAGAGATGATCAACAAGTTCAGCCAAGAGCACAAGATCTCATTTCGAACGATAACTTGCCTGAAAATCAAGAGGAAGAACTGAAGACAATGAAAAGAAAAGCTTCAAATATGGATCTTGAGTTGGATCTATCACTAAAATTAAATTCAAGGAGCAATGGTGATCATAAATGCATAGAGGAGCATGAAATTGATAGCAATTTATCACTCTCTTTGTATTCTCAATCATCTTATGTTTGCAAAAGGATGAAAGAAGAACAAGATAGTTCTAAGGAGCAAGAGAAAGGAGTCAATATTTCTTTGGATCTAACCATATGA
Protein sequence

>Msa1063680

MNMREEETYGSECSKASVSINEDDCDISEGNEDVQKNNNGGISSSNSTIEENCEKKPSVRPYVRSKLPRLRWTPDLHFRFLHAVQRLGGQERATPKLVLQLMNIKGLSIAHVKSHLQMYRSKKVVDTNQVLADHRLLVDNGDRNVYNLTQLPMLQGYTPNQTSSYRCGSYGDASLAMYENMVQMSSISESRAEFYGKMIERTNNSNINIQGHSIFQMDSSNFRELSTTKVHEPNDNFLPFCGHESLRDDQQVQPRAQDLISNDNLPENQEEELKTMKRKASNMDLELDLSLKLNSRSNGDHKCIEEHEIDSNLSLSLYSQSSYVCKRMKEEQDSSKEQEKGVNISLDLTI*