AlfalfaGEDB | Msa1069150

M. sativa cultivar zhongmu-4 / Msa1069150

KEGG: -
GO: GO:0006950 GO:0006952 GO:0006968 GO:0008150 GO:0050896
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069150	AES80579.2	87.310	394	33	1	38	431	24	400	0.0	705

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069150	sp\|Q9FF29\|PR5K_ARATH	56.881	327	128	4	85	408	309	625	5.89e-128	387

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069150	G7KUC9	87.310	394	33	1	38	431	24	400	0.0	705

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1069150	MtrunA17_Chr7g0251071	82.294	401	60	1	29	429	280	669	684
Msa1069150	MtrunA17_Chr7g0251101	81.343	402	71	2	31	429	274	674	675
Msa1069150	MtrunA17_Chr7g0251051	81.704	399	61	2	29	427	240	626	665
Msa1069150	MtrunA17_Chr7g0251061	81.612	397	59	3	32	428	280	662	651
Msa1069150	MtrunA17_Chr7g0251001	80.928	388	63	1	44	431	1	377	646
Msa1069150	MtrunA17_Chr1g0212821	76.368	402	79	3	31	429	9	397	631
Msa1069150	MtrunA17_Chr7g0251081	74.734	376	67	3	31	406	54	401	565
Msa1069150	MtrunA17_Chr7g0250931	69.598	398	105	5	32	428	480	862	565
Msa1069150	MtrunA17_Chr7g0250741	71.000	400	99	6	32	429	465	849	559
Msa1069150	MtrunA17_Chr4g0022331	75.291	344	84	1	70	413	489	831	554

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069150	AT5G38280.1	56.881	327	128	4	85	408	309	625	5.99e-129	387
Msa1069150	AT5G38280.2	56.881	327	128	4	85	408	309	625	1.26e-128	387
Msa1069150	AT1G70250.1	55.723	332	133	5	79	406	431	752	4.68e-126	384
Msa1069150	AT5G38260.1	46.898	403	179	6	27	427	259	628	5.72e-126	379
Msa1069150	AT4G18250.1	56.456	333	128	7	78	403	326	648	1.09e-125	380
Msa1069150	AT5G39020.1	55.077	325	134	4	79	401	466	780	7.64e-123	376
Msa1069150	AT1G66980.4	54.545	330	139	4	93	418	767	1089	2.19e-122	382
Msa1069150	AT1G66980.1	54.545	330	139	4	93	418	784	1106	2.86e-122	382
Msa1069150	AT1G66980.2	54.545	330	139	4	93	418	813	1135	4.77e-122	382
Msa1069150	AT1G66980.3	54.545	330	139	4	93	418	811	1133	4.96e-122	382

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Find 84 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGGTTGACAATAATG+TGG	0.606376	7_2:-64683796	Msa1069150:CDS
AAAGTAATAAACGAGTCAAA+GGG	0.556068	7_2:-64683618	Msa1069150:CDS
AAAGTAATAGAAATGTTGCA+AGG	0.635186	7_2:-64682892	Msa1069150:CDS
AAATTAGGTCAAGGAGGATA+TGG	0.363645	7_2:-64683687	Msa1069150:CDS
AACTCATTTCAAGATAAATT+AGG	0.382330	7_2:-64683702	Msa1069150:CDS
AAGACAGGCTTTGAAGGATA+TGG	0.365162	7_2:+64682853	None:intergenic
AAGAGGAACTGTAGGATATA+TGG	0.395252	7_2:-64683208	Msa1069150:CDS
AAGGCTCTACTAAATATTTC+TGG	0.256593	7_2:+64683183	None:intergenic
AAGTGATGGTCGACAAGTGG+CGG	0.624896	7_2:-64683643	Msa1069150:CDS
AATCATAATGATTATGAACA+CGG	0.673704	7_2:+64683911	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATGTCACTCAACTAAAAA+GGG	+	chr7_2:64683034-64683053	None:intergenic	30.0%
ATACTTTCTATGTTGCACTT+GGG	+	chr7_2:64683951-64683970	None:intergenic	30.0%
AATACTTTCTATGTTGCACT+TGG	+	chr7_2:64683952-64683971	None:intergenic	30.0%
TATATATGACTCTCGATAAG+AGG	-	chr7_2:64684052-64684071	Msa1069150:intron	30.0%
AATGTAACTCCTAGAATTTG+TGG	+	chr7_2:64684123-64684142	None:intergenic	30.0%
CAATTCATTGCTGTCTTTAA+TGG	+	chr7_2:64684172-64684191	None:intergenic	30.0%
GATTTAGGTATGTCACATAT+AGG	+	chr7_2:64684195-64684214	None:intergenic	30.0%
TGGAAGATATAATCTCTTCA+AGG	-	chr7_2:64684340-64684359	Msa1069150:intron	30.0%
TCTGTTCTTTGCATAAATTC+AGG	-	chr7_2:64684732-64684751	Msa1069150:intron	30.0%
AAAACTGGTTGACAATAATG+TGG	-	chr7_2:64684896-64684915	Msa1069150:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	64682765	64685949	64682765	ID=Msa1069150;Name=Msa1069150
chr7_2	mRNA	64682765	64685949	64682765	ID=Msa1069150-mRNA-1;Parent=Msa1069150;Name=Msa1069150-mRNA-1;_AED=0.31;_eAED=0.35;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|0\|431
chr7_2	exon	64685881	64685949	64685881	ID=Msa1069150-mRNA-1:exon:13513;Parent=Msa1069150-mRNA-1
chr7_2	exon	64684061	64684091	64684061	ID=Msa1069150-mRNA-1:exon:13512;Parent=Msa1069150-mRNA-1
chr7_2	exon	64682765	64683960	64682765	ID=Msa1069150-mRNA-1:exon:13511;Parent=Msa1069150-mRNA-1
chr7_2	CDS	64685881	64685949	64685881	ID=Msa1069150-mRNA-1:cds;Parent=Msa1069150-mRNA-1
chr7_2	CDS	64684061	64684091	64684061	ID=Msa1069150-mRNA-1:cds;Parent=Msa1069150-mRNA-1
chr7_2	CDS	64682765	64683960	64682765	ID=Msa1069150-mRNA-1:cds;Parent=Msa1069150-mRNA-1

Gene Sequence

>Msa1069150

ATGGAGAATGCCAGAGTTGCGTTGATTCTGGTGGAGTTTGTGGGAACGATGGAGGAGCTGAATTTAGATGTTCAAAACGGAAACCGAGGGCTTGTAATAGGTGCTTCCGTTGCTTGGTTTGGAGTAACCGTGTTCATAATCATTATGATTAGCTGTCTCTTTAAAAAGAGTAAAGGCAGACAACAAATGAGTATTTTCAAACAACAAAGCCTTATTCCACTTAATATTTTCAGAAAAAGAAGAAAACTGGTTGACAATAATGTGGAGGTTTTCATGCAAAGTTATAAGTTATCAATGCCAAGAAGATATAGTTACACAGAAGTAAAAAGGATCACAAACTCATTTCAAGATAAATTAGGTCAAGGAGGATATGGTGTTGTGTACAAAGCAAGTTTAAGTGATGGTCGACAAGTGGCGGTGAAAGTAATAAACGAGTCAAAGGGAAATGGAGAAGAATTCATAAATGAAGTTGCTAGCATTAGTAGAACATCACACACCAACATTGTCTCACTTATAGGCTTTTGCTATGAGGTGAACAAAAGAGCATTGATATATGAATTCATGTCCAGAGGATCATTGGATAAGTTCATCTATAAAAGCCAATTTCCTAATGCTATTTGTGATTTTGATTGGAACACTTTGTTCCAAATTGCAATTGGCATTGCTAGAGGACTAGAGAACCTGCATCAAGGATGTAGTTCAAGGATTCTGCATCTTGATATCAAACCCCAAAACATTCTTTTGGATGAAGATTTTTGTCCAAAAATATCTGATTTTGGACTAGAAAAAATATGCCGAAAGAATGATAGTACTGTGTCAATGCTAGGGGCAAGAGGAACTGTAGGATATATGGCACCAGAAATATTTAGTAGAGCCTTTGGTGGAGTTTCTCACGAGTCTGATGTGTATAGTTATGGCATGCTGATTCTAGAAATTATCGGAGGTAGGAAGAACTACGACACAGGAGGGTCATGTGCTTCTGAAATGTACTTTCCAGATTGGATTTATAAAGATCTTGAGCAAGATAATGATCCTCTTTTAAATTGTTCGACAATCTCCGAAGAAGAAAATGACATGGTTAGAAAGATTACTATGGCAAGTCTATGGTGCATTCAAACGAATCCATCAGACAGACCACCGATGAATAAAGTAATAGAAATGTTGCAAGGACCACTTTCATCAGTGCCATATCCTTCAAAGCCTGTCTTATTTTCTCCTGAAAGGCCACCACTACAAATGTCACATATATCTTCAACCGACTGGGATGAGACAAATTCAATTACAATATTAAACTGA

Protein sequence

>Msa1069150

MENARVALILVEFVGTMEELNLDVQNGNRGLVIGASVAWFGVTVFIIIMISCLFKKSKGRQQMSIFKQQSLIPLNIFRKRRKLVDNNVEVFMQSYKLSMPRRYSYTEVKRITNSFQDKLGQGGYGVVYKASLSDGRQVAVKVINESKGNGEEFINEVASISRTSHTNIVSLIGFCYEVNKRALIYEFMSRGSLDKFIYKSQFPNAICDFDWNTLFQIAIGIARGLENLHQGCSSRILHLDIKPQNILLDEDFCPKISDFGLEKICRKNDSTVSMLGARGTVGYMAPEIFSRAFGGVSHESDVYSYGMLILEIIGGRKNYDTGGSCASEMYFPDWIYKDLEQDNDPLLNCSTISEEENDMVRKITMASLWCIQTNPSDRPPMNKVIEMLQGPLSSVPYPSKPVLFSPERPPLQMSHISSTDWDETNSITILN*