AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1069740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1069740 MtrunA17_Chr7g0251891 98.990 198 2 0 116 313 7 204 7.73e-148 413
Msa1069740 MtrunA17_Chr7g0251881 87.500 128 8 3 2 125 1 124 3.53e-68 209
Msa1069740 MtrunA17_Chr7g0237841 37.387 222 82 4 66 230 507 728 3.63e-33 129
Msa1069740 MtrunA17_Chr2g0281191 39.394 132 71 3 125 251 352 479 1.86e-20 92.0
Msa1069740 MtrunA17_Chr5g0432721 49.412 85 42 1 125 209 321 404 1.08e-19 89.7
Msa1069740 MtrunA17_Chr4g0012861 39.806 103 61 1 125 227 314 415 1.49e-19 89.4
Msa1069740 MtrunA17_Chr3g0107701 40.777 103 60 1 125 227 338 439 1.76e-19 89.0
Msa1069740 MtrunA17_Chr4g0047031 40.179 112 65 2 118 229 170 279 3.40e-19 86.7
Msa1069740 MtrunA17_Chr7g0244871 43.182 88 49 1 125 212 12 98 1.08e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1069740 AT3G26790.1 46.742 353 108 14 1 313 1 313 1.53e-86 262
Msa1069740 AT3G24650.1 38.785 214 78 5 66 226 459 672 1.25e-35 136
Msa1069740 AT1G28300.1 49.606 127 64 0 117 243 162 288 2.14e-34 129
Msa1069740 AT2G30470.1 39.806 103 61 1 125 227 294 395 8.09e-21 93.6
Msa1069740 AT4G32010.1 34.104 173 98 5 125 294 285 444 1.78e-20 92.4
Msa1069740 AT4G32010.2 34.104 173 98 5 125 294 281 440 2.01e-20 92.4
Msa1069740 AT4G21550.2 38.835 103 60 2 125 227 327 426 7.54e-17 81.3
Msa1069740 AT4G21550.3 38.835 103 60 2 125 227 327 426 7.72e-17 81.3
Msa1069740 AT4G21550.1 38.835 103 60 2 125 227 327 426 8.58e-17 81.3
Msa1069740 AT1G13260.1 35.897 117 67 5 120 231 182 295 4.53e-11 63.5

Find 59 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGTTCTTCAACTCCTTT+TGG 0.172423 7_2:+65532207 None:intergenic
TGATCCAATTCTTGAGTAAT+TGG 0.227933 7_2:+65531157 None:intergenic
ATTATCTTGGTAAACCATAA+TGG 0.319330 7_2:+65531515 None:intergenic
GGTTTAGTTGCTGCTGCATT+TGG 0.326686 7_2:-65534226 Msa1069740:CDS
ATTGGATCAGTTGAAACATT+TGG 0.328924 7_2:-65531143 Msa1069740:CDS
TACATTCGACTGTTATTGTT+AGG 0.354796 7_2:+65531683 None:intergenic
TGCATTACTACAGGTAATTC+AGG 0.355023 7_2:-65531378 Msa1069740:intron
ATGACCAATTACTCAAGAAT+TGG 0.363460 7_2:-65531161 Msa1069740:CDS
ACGCTTTGGAGACTCCATTA+TGG 0.373590 7_2:-65531529 Msa1069740:CDS
GAATTCCTTCCTTCGATTCA+AGG 0.374741 7_2:+65532004 None:intergenic
CTGCATTTGGAACTGTTCAT+AGG 0.379357 7_2:-65534213 Msa1069740:CDS
AACCGAGGCGTGTGGTTTCA+TGG 0.390478 7_2:-65534341 Msa1069740:CDS
AGCACACATGGTCTACGCTT+TGG 0.436188 7_2:-65531543 Msa1069740:CDS
TTGTGATCAAGATGAGTATA+TGG 0.437591 7_2:-65531345 Msa1069740:CDS
AGAGGTGGAATTGGAGAGAT+TGG 0.443660 7_2:+65534091 None:intergenic
CCTTCCTTCGATTCAAGGAC+AGG 0.443951 7_2:+65532009 None:intergenic
GAACTGTTCATAGGAAGAAA+AGG 0.451838 7_2:-65534204 Msa1069740:CDS
GATAGCAATGATGATGGATG+AGG 0.453480 7_2:-65534395 None:intergenic
AAAGTAGATAGCAATGATGA+TGG 0.461976 7_2:-65534401 None:intergenic
GTTTATAGGAAATCGATCAT+AGG 0.469630 7_2:-65532247 Msa1069740:intron
ATATAGATGGTCTTCATGTA+TGG 0.471743 7_2:-65531966 Msa1069740:CDS
TATCTTCGTCGATGATTATG+AGG 0.472524 7_2:-65531297 Msa1069740:CDS
ACAACAACAACAAGATCAAC+GGG 0.481641 7_2:-65534276 Msa1069740:CDS
TCTCTTGATGACTTCTATTG+AGG 0.486619 7_2:-65531104 None:intergenic
ATGTATGGAGTTTCAAGTAC+AGG 0.489515 7_2:-65531951 Msa1069740:intron
GAGGCGTGTGGTTTCATGGC+AGG 0.489847 7_2:-65534337 Msa1069740:CDS
CTCAGCATGAATGATATAGA+TGG 0.492894 7_2:-65531979 Msa1069740:CDS
ATACTTGTATCATTCATTGC+AGG 0.501561 7_2:+65531245 None:intergenic
CCTTCCTGTCCTTGAATCGA+AGG 0.505006 7_2:-65532013 Msa1069740:CDS
TAGGAGAATGGTGTTGCCTA+AGG 0.511895 7_2:-65532171 Msa1069740:intron
AACAACAAGATCAACGGGTC+GGG 0.512814 7_2:-65534271 Msa1069740:CDS
AGATCAACGGGTCGGGTCAA+CGG 0.513632 7_2:-65534264 Msa1069740:CDS
TAGGCAACACCATTCTCCTA+AGG 0.515359 7_2:+65532174 None:intergenic
TGCCATGAAACCACACGCCT+CGG 0.521021 7_2:+65534339 None:intergenic
TGTTAGCTCCCTTAGGAGAA+TGG 0.536299 7_2:-65532183 Msa1069740:CDS
CAACGGATCCATCATGATCA+TGG 0.538714 7_2:-65534247 Msa1069740:CDS
CTAGGGTTTGTCAACGTGAA+AGG 0.540011 7_2:-65534301 Msa1069740:CDS
GGAGACTTTGTTAGCACACA+TGG 0.544519 7_2:-65531555 Msa1069740:CDS
GCAGGTGTTGAAGATGAACT+AGG 0.544873 7_2:-65534319 Msa1069740:CDS
CAACTAAACCATGATCATGA+TGG 0.545398 7_2:+65534239 None:intergenic
ATAGGAAGAAAAGGATGGCT+AGG 0.560627 7_2:-65534195 Msa1069740:CDS
ATAGCAATGATGATGGATGA+GGG 0.562602 7_2:-65534394 None:intergenic
GACAACAACAACAAGATCAA+CGG 0.575839 7_2:-65534277 Msa1069740:CDS
ACAGTGATGTTAGCTCCCTT+AGG 0.583324 7_2:-65532190 Msa1069740:CDS
CAGGTGTTGAAGATGAACTA+GGG 0.583873 7_2:-65534318 Msa1069740:CDS
CCTGTCCTTGAATCGAAGGA+AGG 0.585425 7_2:-65532009 Msa1069740:CDS
CAACAACAAGATCAACGGGT+CGG 0.589941 7_2:-65534272 Msa1069740:CDS
TATAGGAAATCGATCATAGG+AGG 0.597683 7_2:-65532244 Msa1069740:intron
AGAAGGTAAGAAGAAAGTTG+TGG 0.604435 7_2:-65534371 Msa1069740:CDS
AGGCAACACCATTCTCCTAA+GGG 0.607377 7_2:+65532175 None:intergenic
ATGATGATGGATGAGGGAGA+AGG 0.610410 7_2:-65534388 Msa1069740:CDS
CCTTCGATTCAAGGACAGGA+AGG 0.620428 7_2:+65532013 None:intergenic
TGGAGAGATTGGCACGTGCG+AGG 0.625104 7_2:+65534102 None:intergenic
ATGTATGTACTTGAAAACAC+TGG 0.631186 7_2:-65531665 Msa1069740:intron
GTTCAGAAAACCGAGGCGTG+TGG 0.632033 7_2:-65534349 Msa1069740:CDS
TGTTCATAGGAAGAAAAGGA+TGG 0.632115 7_2:-65534200 Msa1069740:CDS
AAGTGGTGAGTCATTTGAGA+TGG 0.633283 7_2:+65531202 None:intergenic
TAACTTGCAGAAAGCAGCAG+AGG 0.635910 7_2:-65532040 Msa1069740:intron
AGTTGTGGTTCAGAAAACCG+AGG 0.753509 7_2:-65534356 Msa1069740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATTTATTATATTATA+AGG + chr7_2:65533433-65533452 None:intergenic 0.0%
!! AAAAAAAAAAAAAAACTTCT+TGG + chr7_2:65531733-65531752 None:intergenic 10.0%
!!! TTTTTATACATATTATTACT+TGG + chr7_2:65532086-65532105 None:intergenic 10.0%
!! AGTAATAATATGTATAAAAA+TGG - chr7_2:65532086-65532105 Msa1069740:intron 10.0%
!!! TTAAAATATTTGATGATTAT+TGG - chr7_2:65532915-65532934 Msa1069740:intron 10.0%
!! AAAATCTATACAATTAACAT+CGG + chr7_2:65531612-65531631 None:intergenic 15.0%
!! ATATGTATAAAAATGGAAAA+AGG - chr7_2:65532093-65532112 Msa1069740:intron 15.0%
!!! TGAATGAATAAACATTATTT+TGG + chr7_2:65532198-65532217 None:intergenic 15.0%
!! TGTTAATTAATACTATTCAT+CGG - chr7_2:65532256-65532275 Msa1069740:intron 15.0%
!!! AATTACGAAAGTTAATTTTA+CGG - chr7_2:65532651-65532670 Msa1069740:intron 15.0%
!! TGTTGGATATAAATATAATA+TGG - chr7_2:65533059-65533078 Msa1069740:intron 15.0%
!! AAATTAGTTAATACCATAAA+TGG - chr7_2:65533205-65533224 Msa1069740:intron 15.0%
!! ATTATATTATAAGGTTATGA+TGG + chr7_2:65533424-65533443 None:intergenic 15.0%
!! AAATTTAATGCAATTACATT+CGG - chr7_2:65533624-65533643 Msa1069740:intron 15.0%
!!! TTAGTTTGTTAAATATTTTC+AGG - chr7_2:65533918-65533937 Msa1069740:intron 15.0%
!! CAATTCAAAAGATTAATCTT+TGG + chr7_2:65531790-65531809 None:intergenic 20.0%
!!! CAAAGATTAATCTTTTGAAT+TGG - chr7_2:65531788-65531807 Msa1069740:intron 20.0%
!! ATAATTAAATAAAAGGGAAG+TGG - chr7_2:65531949-65531968 Msa1069740:intron 20.0%
!!! AAGTAATGCTTATAGAATTT+TGG - chr7_2:65532301-65532320 Msa1069740:intron 20.0%
!! TTAACTATATCAATGTGTAT+AGG - chr7_2:65532342-65532361 Msa1069740:intron 20.0%
!!! ATTACGAAAGTTAATTTTAC+GGG - chr7_2:65532652-65532671 Msa1069740:intron 20.0%
!!! AAATATTTGATGATTATTGG+TGG - chr7_2:65532918-65532937 Msa1069740:intron 20.0%
!!! ACAATTTTCATTCTGTTTAT+AGG - chr7_2:65533233-65533252 Msa1069740:intron 20.0%
!! AATTGCATTAAATTTCATTC+TGG + chr7_2:65533619-65533638 None:intergenic 20.0%
!!! AATTGTACTTTAAAGTCATT+TGG - chr7_2:65533712-65533731 Msa1069740:intron 20.0%
!!! ATTGTACTTTAAAGTCATTT+GGG - chr7_2:65533713-65533732 Msa1069740:intron 20.0%
!!! AATTGTGATTTTGATTATCT+TGG + chr7_2:65533995-65534014 None:intergenic 20.0%
!!! ATTTTTGAGATGAATAGTGA+TGG + chr7_2:65531347-65531366 None:intergenic 25.0%
!! TTTTTATTATCTGTCCTAGT+TGG - chr7_2:65531506-65531525 Msa1069740:CDS 25.0%
! TACTAGTAGTTTGATCTTAA+TGG - chr7_2:65531670-65531689 Msa1069740:CDS 25.0%
!! TTATCTTCATTTTCTTGTGT+GGG + chr7_2:65531896-65531915 None:intergenic 25.0%
!! TTTATCTTCATTTTCTTGTG+TGG + chr7_2:65531897-65531916 None:intergenic 25.0%
! ATAAAAATGGAAAAAGGAAG+AGG - chr7_2:65532099-65532118 Msa1069740:intron 25.0%
! ATAGTTAAACCAAAAAGACA+AGG + chr7_2:65532330-65532349 None:intergenic 25.0%
! TTACTCAATTGTCTATTCTA+TGG - chr7_2:65532365-65532384 Msa1069740:intron 25.0%
! TACTCAATTGTCTATTCTAT+GGG - chr7_2:65532366-65532385 Msa1069740:intron 25.0%
! AATTCAGAAGTCAATTTCAT+AGG - chr7_2:65532488-65532507 Msa1069740:intron 25.0%
!!! ATTTCGAAAGTCGATTTTAT+TGG - chr7_2:65532586-65532605 Msa1069740:intron 25.0%
! TAAATAGAGCCTTTACATTT+GGG - chr7_2:65532941-65532960 Msa1069740:intron 25.0%
! ACATAAATTCCCAAATGTAA+AGG + chr7_2:65532953-65532972 None:intergenic 25.0%
! AAACAGAATGAAAATTGTCA+AGG + chr7_2:65533231-65533250 None:intergenic 25.0%
!! TATTTCTATTTCAATACTGC+AGG - chr7_2:65533782-65533801 Msa1069740:intron 25.0%
! ATTATCTTGGTAAACCATAA+TGG + chr7_2:65533982-65534001 None:intergenic 25.0%
! TGACAGGAAAAAAAAATACA+TGG + chr7_2:65534094-65534113 None:intergenic 25.0%
! AAGTTTTTGCAAATCCAACT+AGG + chr7_2:65531523-65531542 None:intergenic 30.0%
!! TTTTAACTCTTTGCTAGAAG+AGG - chr7_2:65532123-65532142 Msa1069740:intron 30.0%
!! CTACTCATTTTAGAAGTCAA+CGG - chr7_2:65532409-65532428 Msa1069740:intron 30.0%
GGTCAACTATATCATGTATA+CGG - chr7_2:65532509-65532528 Msa1069740:intron 30.0%
!! ATTTCGGAAGTCGATTTTAT+GGG - chr7_2:65532537-65532556 Msa1069740:intron 30.0%
!! AAGTCGATTTTATTGGTCAA+CGG - chr7_2:65532593-65532612 Msa1069740:intron 30.0%
! AAGTTAATTTTACGGGTCAA+CGG - chr7_2:65532659-65532678 Msa1069740:intron 30.0%
ATCGAGTCTATCTAAGTTAT+TGG + chr7_2:65532782-65532801 None:intergenic 30.0%
GTAAATAGAGCCTTTACATT+TGG - chr7_2:65532940-65532959 Msa1069740:intron 30.0%
GTATTTCAAGATAAGCCATA+AGG - chr7_2:65532971-65532990 Msa1069740:intron 30.0%
AGTAAATGCTGCAAAAAGTT+GGG + chr7_2:65533152-65533171 None:intergenic 30.0%
TAGTAAATGCTGCAAAAAGT+TGG + chr7_2:65533153-65533172 None:intergenic 30.0%
AAAATTGTCAAGGCCATTTA+TGG + chr7_2:65533221-65533240 None:intergenic 30.0%
GTTTATAGGAAATCGATCAT+AGG - chr7_2:65533247-65533266 Msa1069740:intron 30.0%
GAAATGAAGTCTATAAGCAA+AGG + chr7_2:65533354-65533373 None:intergenic 30.0%
ATATAGATGGTCTTCATGTA+TGG - chr7_2:65533528-65533547 Msa1069740:intron 30.0%
GTCAATCATTCTTTATCTGA+CGG + chr7_2:65533682-65533701 None:intergenic 30.0%
! TATTTCAATACTGCAGGTTT+TGG - chr7_2:65533788-65533807 Msa1069740:intron 30.0%
TACATTCGACTGTTATTGTT+AGG + chr7_2:65533814-65533833 None:intergenic 30.0%
ATGTATGTACTTGAAAACAC+TGG - chr7_2:65533829-65533848 Msa1069740:intron 30.0%
AGCAAAGTTTAACTCTCAAA+TGG + chr7_2:65533888-65533907 None:intergenic 30.0%
TTGTGATCAAGATGAGTATA+TGG - chr7_2:65534149-65534168 Msa1069740:CDS 30.0%
ATACTTGTATCATTCATTGC+AGG + chr7_2:65534252-65534271 None:intergenic 30.0%
ATGACCAATTACTCAAGAAT+TGG - chr7_2:65534333-65534352 Msa1069740:CDS 30.0%
TGATCCAATTCTTGAGTAAT+TGG + chr7_2:65534340-65534359 None:intergenic 30.0%
ATTGGATCAGTTGAAACATT+TGG - chr7_2:65534351-65534370 Msa1069740:CDS 30.0%
AGAAGGTAAGAAGAAAGTTG+TGG - chr7_2:65531123-65531142 Msa1069740:CDS 35.0%
GACAACAACAACAAGATCAA+CGG - chr7_2:65531217-65531236 Msa1069740:CDS 35.0%
ACAACAACAACAAGATCAAC+GGG - chr7_2:65531218-65531237 Msa1069740:CDS 35.0%
CAACTAAACCATGATCATGA+TGG + chr7_2:65531258-65531277 None:intergenic 35.0%
GAACTGTTCATAGGAAGAAA+AGG - chr7_2:65531290-65531309 Msa1069740:CDS 35.0%
TGTTCATAGGAAGAAAAGGA+TGG - chr7_2:65531294-65531313 Msa1069740:CDS 35.0%
GTAGTAGTAGTAGTAGAAGA+AGG + chr7_2:65531373-65531392 None:intergenic 35.0%
GAATGAAAAAGAGGTGGAAT+TGG + chr7_2:65531415-65531434 None:intergenic 35.0%
AGAAAAAAAACTCACACGTG+CGG + chr7_2:65531446-65531465 None:intergenic 35.0%
AAGAACTTCGTCATCTATTG+TGG + chr7_2:65531820-65531839 None:intergenic 35.0%
!! CAACGGTGTTAAGTCATTTT+AGG + chr7_2:65531843-65531862 None:intergenic 35.0%
GGGTGTGTATAAATTAGTGT+AGG + chr7_2:65531876-65531895 None:intergenic 35.0%
ATAAAAGGGAAGTGGATGTA+TGG - chr7_2:65531957-65531976 Msa1069740:CDS 35.0%
TAAAAGGGAAGTGGATGTAT+GGG - chr7_2:65531958-65531977 Msa1069740:CDS 35.0%
!! TTTTGGTGACCTTGTCTTTT+TGG - chr7_2:65532318-65532337 Msa1069740:intron 35.0%
CACGTATATGCTACTCATTT+CGG - chr7_2:65532435-65532454 Msa1069740:intron 35.0%
TATATGCTACTCATTTCGGA+AGG - chr7_2:65532439-65532458 Msa1069740:intron 35.0%
!! CATTTCGGAAGTCGATTTTA+TGG - chr7_2:65532536-65532555 Msa1069740:intron 35.0%
TCTCGATCGTTTATAGAGAT+CGG - chr7_2:65532698-65532717 Msa1069740:intron 35.0%
! AAGCTGAAAACTCCTACTTT+TGG - chr7_2:65532865-65532884 Msa1069740:intron 35.0%
AGATTTAGGGAACCAAAAGT+AGG + chr7_2:65532880-65532899 None:intergenic 35.0%
!!! CTTTTGGTTCCCTAAATCTT+TGG - chr7_2:65532881-65532900 Msa1069740:intron 35.0%
TTTACTCGATGCTCTTATGT+TGG - chr7_2:65533042-65533061 Msa1069740:intron 35.0%
TATAGGAAATCGATCATAGG+AGG - chr7_2:65533250-65533269 Msa1069740:intron 35.0%
! GTTGAGATTCCTTTTCCAAA+AGG - chr7_2:65533272-65533291 Msa1069740:intron 35.0%
!! TCTTCAACTCCTTTTGGAAA+AGG + chr7_2:65533284-65533303 None:intergenic 35.0%
CTCAGCATGAATGATATAGA+TGG - chr7_2:65533515-65533534 Msa1069740:intron 35.0%
! ATGTATGGAGTTTCAAGTAC+AGG - chr7_2:65533543-65533562 Msa1069740:intron 35.0%
TGCATTACTACAGGTAATTC+AGG - chr7_2:65534116-65534135 Msa1069740:CDS 35.0%
TATCTTCGTCGATGATTATG+AGG - chr7_2:65534197-65534216 Msa1069740:CDS 35.0%
!!! TGACTCACCACTTGATTTTT+TGG - chr7_2:65534302-65534321 Msa1069740:CDS 35.0%
!!! GACTCACCACTTGATTTTTT+GGG - chr7_2:65534303-65534322 Msa1069740:CDS 35.0%
!! CAGGTGTTGAAGATGAACTA+GGG - chr7_2:65531176-65531195 Msa1069740:CDS 40.0%
! CTGCATTTGGAACTGTTCAT+AGG - chr7_2:65531281-65531300 Msa1069740:CDS 40.0%
ATAGGAAGAAAAGGATGGCT+AGG - chr7_2:65531299-65531318 Msa1069740:CDS 40.0%
GAAAAAAAACTCACACGTGC+GGG + chr7_2:65531445-65531464 None:intergenic 40.0%
GTGTAGGTCTGAGAATACAA+CGG + chr7_2:65531860-65531879 None:intergenic 40.0%
AGGCAGAATCTATGTCTGTA+CGG - chr7_2:65532143-65532162 Msa1069740:intron 40.0%
!!! ATTATTTTGGTCATTGCCCG+TGG + chr7_2:65532185-65532204 None:intergenic 40.0%
CATGTATACGGCACTCATTT+CGG - chr7_2:65532521-65532540 Msa1069740:intron 40.0%
TCACGTGACTTTCGCAAATT+GGG + chr7_2:65532743-65532762 None:intergenic 40.0%
CTTAGTCAATGTCTAGTCCT+TGG - chr7_2:65532842-65532861 Msa1069740:intron 40.0%
TTGGTTCCCTAAATCTTTGG+TGG - chr7_2:65532884-65532903 Msa1069740:intron 40.0%
ACACCACCACCAAAGATTTA+GGG + chr7_2:65532893-65532912 None:intergenic 40.0%
AAAGAGTAACGGTGGTAAAG+AGG + chr7_2:65533025-65533044 None:intergenic 40.0%
!! GAGCATCGAGTAAAGAGTAA+CGG + chr7_2:65533036-65533055 None:intergenic 40.0%
! CACTGTTCTTCAACTCCTTT+TGG + chr7_2:65533290-65533309 None:intergenic 40.0%
ATAAGCAAAGGTCAGACCTT+AGG + chr7_2:65533342-65533361 None:intergenic 40.0%
GAATTCCTTCCTTCGATTCA+AGG + chr7_2:65533493-65533512 None:intergenic 40.0%
CTGCTATGTGCATACATTGT+TGG - chr7_2:65533567-65533586 Msa1069740:intron 40.0%
CCTGTAGTAATGCATATGAC+AGG + chr7_2:65534110-65534129 None:intergenic 40.0%
CCTGTCATATGCATTACTAC+AGG - chr7_2:65534107-65534126 Msa1069740:CDS 40.0%
AAGTGGTGAGTCATTTGAGA+TGG + chr7_2:65534295-65534314 None:intergenic 40.0%
!!! TCACCACTTGATTTTTTGGG+TGG - chr7_2:65534306-65534325 Msa1069740:CDS 40.0%
GATCCACCCAAAAAATCAAG+TGG + chr7_2:65534312-65534331 None:intergenic 40.0%
!! ATGATGATGGATGAGGGAGA+AGG - chr7_2:65531106-65531125 Msa1069740:CDS 45.0%
AGTTGTGGTTCAGAAAACCG+AGG - chr7_2:65531138-65531157 Msa1069740:CDS 45.0%
!! GCAGGTGTTGAAGATGAACT+AGG - chr7_2:65531175-65531194 Msa1069740:CDS 45.0%
CTAGGGTTTGTCAACGTGAA+AGG - chr7_2:65531193-65531212 Msa1069740:CDS 45.0%
CAACAACAAGATCAACGGGT+CGG - chr7_2:65531222-65531241 Msa1069740:CDS 45.0%
AACAACAAGATCAACGGGTC+GGG - chr7_2:65531223-65531242 Msa1069740:CDS 45.0%
CAACGGATCCATCATGATCA+TGG - chr7_2:65531247-65531266 Msa1069740:CDS 45.0%
GGTTTAGTTGCTGCTGCATT+TGG - chr7_2:65531268-65531287 Msa1069740:CDS 45.0%
AGAGGTGGAATTGGAGAGAT+TGG + chr7_2:65531406-65531425 None:intergenic 45.0%
GGGAGAGAATGAAAAAGAGG+TGG + chr7_2:65531421-65531440 None:intergenic 45.0%
GGAGGGAGAGAATGAAAAAG+AGG + chr7_2:65531424-65531443 None:intergenic 45.0%
CTCACGTGACTTTCGCAAAT+TGG + chr7_2:65532744-65532763 None:intergenic 45.0%
! GTAGGAGTTTTCAGCTTCCA+AGG + chr7_2:65532862-65532881 None:intergenic 45.0%
GTTCCCTAAATCTTTGGTGG+TGG - chr7_2:65532887-65532906 Msa1069740:intron 45.0%
CACACCACCACCAAAGATTT+AGG + chr7_2:65532894-65532913 None:intergenic 45.0%
!! CTAAATCTTTGGTGGTGGTG+TGG - chr7_2:65532892-65532911 Msa1069740:intron 45.0%
GCATTGTCTAGCATGCCTTA+TGG + chr7_2:65532989-65533008 None:intergenic 45.0%
AGGCATGCTAGACAATGCTT+TGG - chr7_2:65532991-65533010 Msa1069740:intron 45.0%
GGCATGCTAGACAATGCTTT+GGG - chr7_2:65532992-65533011 Msa1069740:intron 45.0%
CATCGAGTAAAGAGTAACGG+TGG + chr7_2:65533033-65533052 None:intergenic 45.0%
ACAGTGATGTTAGCTCCCTT+AGG - chr7_2:65533304-65533323 Msa1069740:intron 45.0%
TGTTAGCTCCCTTAGGAGAA+TGG - chr7_2:65533311-65533330 Msa1069740:intron 45.0%
AGGCAACACCATTCTCCTAA+GGG + chr7_2:65533322-65533341 None:intergenic 45.0%
TAGGCAACACCATTCTCCTA+AGG + chr7_2:65533323-65533342 None:intergenic 45.0%
!! TAGGAGAATGGTGTTGCCTA+AGG - chr7_2:65533323-65533342 Msa1069740:intron 45.0%
! TAACTTGCAGAAAGCAGCAG+AGG - chr7_2:65533454-65533473 Msa1069740:intron 45.0%
GGAGACTTTGTTAGCACACA+TGG - chr7_2:65533939-65533958 Msa1069740:intron 45.0%
!! ACGCTTTGGAGACTCCATTA+TGG - chr7_2:65533965-65533984 Msa1069740:intron 45.0%
!! AAATACAATAATTAAATAAA+AGG - chr7_2:65531942-65531961 Msa1069740:intron 5.0%
!! AATACAATAATTAAATAAAA+GGG - chr7_2:65531943-65531962 Msa1069740:intron 5.0%
AAAAACTCACACGTGCGGGA+GGG + chr7_2:65531441-65531460 None:intergenic 50.0%
AAAAAACTCACACGTGCGGG+AGG + chr7_2:65531442-65531461 None:intergenic 50.0%
TCTGTACGGAATTTGCCTCG+TGG - chr7_2:65532157-65532176 Msa1069740:intron 50.0%
GAATTTGCCTCGTGGATCCA+CGG - chr7_2:65532165-65532184 Msa1069740:intron 50.0%
AATTTGCCTCGTGGATCCAC+GGG - chr7_2:65532166-65532185 Msa1069740:intron 50.0%
CCTTCGATTCAAGGACAGGA+AGG + chr7_2:65533484-65533503 None:intergenic 50.0%
CCTTCCTGTCCTTGAATCGA+AGG - chr7_2:65533481-65533500 Msa1069740:intron 50.0%
CCTTCCTTCGATTCAAGGAC+AGG + chr7_2:65533488-65533507 None:intergenic 50.0%
CCTGTCCTTGAATCGAAGGA+AGG - chr7_2:65533485-65533504 Msa1069740:intron 50.0%
AGCACACATGGTCTACGCTT+TGG - chr7_2:65533951-65533970 Msa1069740:intron 50.0%
GTTCAGAAAACCGAGGCGTG+TGG - chr7_2:65531145-65531164 Msa1069740:CDS 55.0%
AACCGAGGCGTGTGGTTTCA+TGG - chr7_2:65531153-65531172 Msa1069740:CDS 55.0%
TGCCATGAAACCACACGCCT+CGG + chr7_2:65531158-65531177 None:intergenic 55.0%
AGATCAACGGGTCGGGTCAA+CGG - chr7_2:65531230-65531249 Msa1069740:CDS 55.0%
GAGGCGTGTGGTTTCATGGC+AGG - chr7_2:65531157-65531176 Msa1069740:CDS 60.0%
TGGAGAGATTGGCACGTGCG+AGG + chr7_2:65531395-65531414 None:intergenic 60.0%
TCATTGCCCGTGGATCCACG+AGG + chr7_2:65532175-65532194 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 65531106 65534410 65531106 ID=Msa1069740;Name=Msa1069740
chr7_2 mRNA 65531106 65534410 65531106 ID=Msa1069740-mRNA-1;Parent=Msa1069740;Name=Msa1069740-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|6|0|313
chr7_2 exon 65534066 65534410 65534066 ID=Msa1069740-mRNA-1:exon:13808;Parent=Msa1069740-mRNA-1
chr7_2 exon 65532172 65532261 65532172 ID=Msa1069740-mRNA-1:exon:13807;Parent=Msa1069740-mRNA-1
chr7_2 exon 65531952 65532052 65531952 ID=Msa1069740-mRNA-1:exon:13806;Parent=Msa1069740-mRNA-1
chr7_2 exon 65531666 65531712 65531666 ID=Msa1069740-mRNA-1:exon:13805;Parent=Msa1069740-mRNA-1
chr7_2 exon 65531500 65531576 65531500 ID=Msa1069740-mRNA-1:exon:13804;Parent=Msa1069740-mRNA-1
chr7_2 exon 65531106 65531387 65531106 ID=Msa1069740-mRNA-1:exon:13803;Parent=Msa1069740-mRNA-1
chr7_2 CDS 65534066 65534410 65534066 ID=Msa1069740-mRNA-1:cds;Parent=Msa1069740-mRNA-1
chr7_2 CDS 65532172 65532261 65532172 ID=Msa1069740-mRNA-1:cds;Parent=Msa1069740-mRNA-1
chr7_2 CDS 65531952 65532052 65531952 ID=Msa1069740-mRNA-1:cds;Parent=Msa1069740-mRNA-1
chr7_2 CDS 65531666 65531712 65531666 ID=Msa1069740-mRNA-1:cds;Parent=Msa1069740-mRNA-1
chr7_2 CDS 65531500 65531576 65531500 ID=Msa1069740-mRNA-1:cds;Parent=Msa1069740-mRNA-1
chr7_2 CDS 65531106 65531387 65531106 ID=Msa1069740-mRNA-1:cds;Parent=Msa1069740-mRNA-1
Gene Sequence

>Msa1069740

ATGATGATGGATGAGGGAGAAGGTAAGAAGAAAGTTGTGGTTCAGAAAACCGAGGCGTGTGGTTTCATGGCAGGTGTTGAAGATGAACTAGGGTTTGTCAACGTGAAAGGAGACAACAACAACAAGATCAACGGGTCGGGTCAACGGATCCATCATGATCATGGTTTAGTTGCTGCTGCATTTGGAACTGTTCATAGGAAGAAAAGGATGGCTAGGCAAAGAAGATCTTCATCTTCAACCATCACTATTCATCTCAAAAATCTTCCTTCTTCTACTACTACTACTACCTCGCACGTGCCAATCTCTCCAATTCCACCTCTTTTTCATTCTCTCCCTCCCGCACGTGAAATCGATCATAGGAGGTTGAGATTCCTTTTCCAAAAGGAGTTGAAGAACAGTGATGTTAGCTCCCTTAGGAGAATGGTGTTGCCTAAGAAAGCAGCAGAGGCTTTCCTTCCTGTCCTTGAATCGAAGGAAGGAATTCTTCTCAGCATGAATGATATAGATGGTCTTCATGTATGGAGTTTCAAGTACAGGTTTTGGCCTAACAATAACAGTCGAATGTATGTACTTGAAAACACTGGAGACTTTGTTAGCACACATGGTCTACGCTTTGGAGACTCCATTATGGTTTACCAAGATAATCAAAATCACAATTATGTAATTCAGGCAAAGAAAGCTTGTGATCAAGATGAGTATATGGAAGAAGCTAATGATACAATCAATCATATCTTCGTCGATGATTATGAGGTGAACAAAAGTTGCTTTGATGTAGCTTATCCTGCAATGAATGATACAAGTATGTCATTTATATATGACACAACCATCTCAAATGACTCACCACTTGATTTTTTGGGTGGATCAATGACCAATTACTCAAGAATTGGATCAGTTGAAACATTTGGCTCTGTTGAGAATCTGTCTCTTGATGACTTCTATTGA
Protein sequence

>Msa1069740

MMMDEGEGKKKVVVQKTEACGFMAGVEDELGFVNVKGDNNNKINGSGQRIHHDHGLVAAAFGTVHRKKRMARQRRSSSSTITIHLKNLPSSTTTTTSHVPISPIPPLFHSLPPAREIDHRRLRFLFQKELKNSDVSSLRRMVLPKKAAEAFLPVLESKEGILLSMNDIDGLHVWSFKYRFWPNNNSRMYVLENTGDFVSTHGLRFGDSIMVYQDNQNHNYVIQAKKACDQDEYMEEANDTINHIFVDDYEVNKSCFDVAYPAMNDTSMSFIYDTTISNDSPLDFLGGSMTNYSRIGSVETFGSVENLSLDDFY*