Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1071610 | XP_039683935.1 | 91.026 | 234 | 18 | 1 | 1 | 231 | 1 | 234 | 3.62e-149 | 424 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1071610 | A0A396H4J8 | 91.026 | 234 | 18 | 1 | 1 | 231 | 1 | 234 | 1.73e-149 | 424 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1071610 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1071610 | MtrunA17_Chr7g0255511 | 91.026 | 234 | 18 | 1 | 1 | 231 | 1 | 234 | 3.33e-153 | 424 |
| Msa1071610 | MtrunA17_Chr7g0225091 | 79.060 | 234 | 32 | 2 | 1 | 231 | 1 | 220 | 5.41e-132 | 370 |
| Msa1071610 | MtrunA17_Chr7g0225121 | 75.214 | 234 | 33 | 2 | 1 | 231 | 1 | 212 | 1.80e-124 | 351 |
| Msa1071610 | MtrunA17_Chr7g0222361 | 79.208 | 202 | 41 | 1 | 31 | 231 | 1 | 202 | 8.69e-121 | 341 |
| Msa1071610 | MtrunA17_Chr7g0222371 | 75.207 | 121 | 26 | 2 | 19 | 135 | 1 | 121 | 2.82e-62 | 191 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1071610 | AT1G66140.1 | 29.121 | 182 | 99 | 4 | 77 | 231 | 82 | 260 | 6.02e-11 | 61.2 |
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGTGGATATCATTATTA+TGG | 0.231665 | 7_2:-68370521 | Msa1071610:CDS |
| CTCCTTCCCCTTGTTGCACA+TGG | 0.338672 | 7_2:+68370940 | None:intergenic |
| TATATAATAATCAAAGCTTA+AGG | 0.354043 | 7_2:+68370255 | None:intergenic |
| TCCAAGCATTGTTAAAAGCT+TGG | 0.375606 | 7_2:+68370603 | None:intergenic |
| CACCATCTCTCCATTCCATA+TGG | 0.384400 | 7_2:-68370543 | Msa1071610:CDS |
| CTTAAGGGTCAAATCAATCT+TGG | 0.396784 | 7_2:+68370271 | None:intergenic |
| AATGATATCCACACCCCATA+TGG | 0.402297 | 7_2:+68370528 | None:intergenic |
| AATTACTCAAATGGTGATCA+AGG | 0.411277 | 7_2:-68370578 | Msa1071610:CDS |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7_2:-68370494 | Msa1071610:CDS |
| ATGTTGAAACTCAGAATTCA+AGG | 0.446337 | 7_2:-68370835 | Msa1071610:CDS |
| GCTTGGATAAATTACTCAAA+TGG | 0.450589 | 7_2:-68370587 | Msa1071610:CDS |
| AGTAGCCAATTTGAAGATGA+TGG | 0.452167 | 7_2:-68370900 | Msa1071610:CDS |
| AATATGTCTGAGATTGAAGT+TGG | 0.464395 | 7_2:-68370368 | Msa1071610:CDS |
| TCATCTGCTTCATCTATCTC+TGG | 0.464587 | 7_2:+68370422 | None:intergenic |
| ACCATCTCTCCATTCCATAT+GGG | 0.464694 | 7_2:-68370542 | Msa1071610:CDS |
| TCACAGGAAAAGTATTCAAA+AGG | 0.468253 | 7_2:-68370726 | Msa1071610:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7_2:-68370674 | Msa1071610:CDS |
| TGAAGTTGGTTCAGTTTCAA+AGG | 0.476665 | 7_2:-68370354 | Msa1071610:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7_2:-68370742 | Msa1071610:CDS |
| TGTTAGTGATGAAGTTGATC+AGG | 0.482731 | 7_2:-68370324 | Msa1071610:CDS |
| CAAGAGAAAGGCAATTGTGC+TGG | 0.501297 | 7_2:-68370867 | Msa1071610:CDS |
| CTTTGATGCCATGTGCAACA+AGG | 0.514941 | 7_2:-68370948 | None:intergenic |
| ATATAATAATCAAAGCTTAA+GGG | 0.520066 | 7_2:+68370256 | None:intergenic |
| GAAGTTGGTTCAGTTTCAAA+GGG | 0.529917 | 7_2:-68370353 | Msa1071610:CDS |
| TCTCCATTCCATATGGGGTG+TGG | 0.536840 | 7_2:-68370536 | Msa1071610:CDS |
| GGAAAATCAGAACAAGAGAA+AGG | 0.539450 | 7_2:-68370879 | Msa1071610:CDS |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7_2:-68370493 | Msa1071610:CDS |
| CCCCATATGGAATGGAGAGA+TGG | 0.561790 | 7_2:+68370541 | None:intergenic |
| TGCCATGTGCAACAAGGGGA+AGG | 0.572549 | 7_2:-68370942 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7_2:-68370681 | Msa1071610:CDS |
| TTTGATGCCATGTGCAACAA+GGG | 0.577704 | 7_2:-68370947 | None:intergenic |
| GTGCAACAAGGGGAAGGAGA+AGG | 0.585340 | 7_2:-68370936 | Msa1071610:CDS |
| GTTGGTTCAGTTTCAAAGGG+AGG | 0.593873 | 7_2:-68370350 | Msa1071610:CDS |
| TATCCACACCCCATATGGAA+TGG | 0.594771 | 7_2:+68370533 | None:intergenic |
| CCATCTCTCCATTCCATATG+GGG | 0.598148 | 7_2:-68370541 | Msa1071610:CDS |
| ATTACTCAAATGGTGATCAA+GGG | 0.605537 | 7_2:-68370577 | Msa1071610:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7_2:-68370303 | Msa1071610:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.637178 | 7_2:+68370665 | None:intergenic |
| TTGATGCCATGTGCAACAAG+GGG | 0.659240 | 7_2:-68370946 | None:intergenic |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7_2:-68370671 | Msa1071610:CDS |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7_2:-68370300 | Msa1071610:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTTCCATCATCTTCAAAT+TGG | + | chr7_2:68370311-68370330 | None:intergenic | 25.0% |
| !! | TTCTTCAACAACATCTTTTT+GGG | + | chr7_2:68370398-68370417 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | - | chr7_2:68370442-68370461 | Msa1071610:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | + | chr7_2:68370451-68370470 | None:intergenic | 25.0% |
| ATGTTGAAACTCAGAATTCA+AGG | - | chr7_2:68370368-68370387 | Msa1071610:CDS | 30.0% | |
| ! | TCTTCAACAACATCTTTTTG+GGG | + | chr7_2:68370397-68370416 | None:intergenic | 30.0% |
| ! | CTTCTTCAACAACATCTTTT+TGG | + | chr7_2:68370399-68370418 | None:intergenic | 30.0% |
| !! | TCACAGGAAAAGTATTCAAA+AGG | - | chr7_2:68370477-68370496 | Msa1071610:CDS | 30.0% |
| TAGAGAGAAGTCTCAAAAAA+AGG | - | chr7_2:68370575-68370594 | Msa1071610:CDS | 30.0% | |
| GCTTGGATAAATTACTCAAA+TGG | - | chr7_2:68370616-68370635 | Msa1071610:CDS | 30.0% | |
| AATTACTCAAATGGTGATCA+AGG | - | chr7_2:68370625-68370644 | Msa1071610:CDS | 30.0% | |
| ATTACTCAAATGGTGATCAA+GGG | - | chr7_2:68370626-68370645 | Msa1071610:CDS | 30.0% | |
| ! | TTTTATGCAGGGAATTTGTA+TGG | - | chr7_2:68370742-68370761 | Msa1071610:CDS | 30.0% |
| ! | AATATGTCTGAGATTGAAGT+TGG | - | chr7_2:68370835-68370854 | Msa1071610:CDS | 30.0% |
| AGTAGCCAATTTGAAGATGA+TGG | - | chr7_2:68370303-68370322 | Msa1071610:CDS | 35.0% | |
| GGAAAATCAGAACAAGAGAA+AGG | - | chr7_2:68370324-68370343 | Msa1071610:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAC+AGG | - | chr7_2:68370461-68370480 | Msa1071610:CDS | 35.0% | |
| ! | TCCAAGCTTTTAACAATGCT+TGG | - | chr7_2:68370599-68370618 | Msa1071610:CDS | 35.0% |
| ! | TCCAAGCATTGTTAAAAGCT+TGG | + | chr7_2:68370603-68370622 | None:intergenic | 35.0% |
| GGGTGTGGATATCATTATTA+TGG | - | chr7_2:68370682-68370701 | Msa1071610:CDS | 35.0% | |
| AACAATATGATTCAGCATGC+AGG | - | chr7_2:68370709-68370728 | Msa1071610:CDS | 35.0% | |
| ACAATATGATTCAGCATGCA+GGG | - | chr7_2:68370710-68370729 | Msa1071610:CDS | 35.0% | |
| ! | GGTCTACAAACTTTTATGCA+GGG | - | chr7_2:68370731-68370750 | Msa1071610:CDS | 35.0% |
| ! | TGAAGTTGGTTCAGTTTCAA+AGG | - | chr7_2:68370849-68370868 | Msa1071610:CDS | 35.0% |
| ! | GAAGTTGGTTCAGTTTCAAA+GGG | - | chr7_2:68370850-68370869 | Msa1071610:CDS | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | - | chr7_2:68370879-68370898 | Msa1071610:CDS | 35.0% |
| CTTAAGGGTCAAATCAATCT+TGG | + | chr7_2:68370935-68370954 | None:intergenic | 35.0% | |
| ACCATCTCTCCATTCCATAT+GGG | - | chr7_2:68370661-68370680 | Msa1071610:CDS | 40.0% | |
| AATGATATCCACACCCCATA+TGG | + | chr7_2:68370678-68370697 | None:intergenic | 40.0% | |
| ! | GGGTCTACAAACTTTTATGC+AGG | - | chr7_2:68370730-68370749 | Msa1071610:CDS | 40.0% |
| TCATCTGCTTCATCTATCTC+TGG | + | chr7_2:68370784-68370803 | None:intergenic | 40.0% | |
| CAAGAGAAAGGCAATTGTGC+TGG | - | chr7_2:68370336-68370355 | Msa1071610:CDS | 45.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | - | chr7_2:68370522-68370541 | Msa1071610:CDS | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | - | chr7_2:68370529-68370548 | Msa1071610:CDS | 45.0% | |
| CACCATCTCTCCATTCCATA+TGG | - | chr7_2:68370660-68370679 | Msa1071610:CDS | 45.0% | |
| CCATCTCTCCATTCCATATG+GGG | - | chr7_2:68370662-68370681 | Msa1071610:CDS | 45.0% | |
| TATCCACACCCCATATGGAA+TGG | + | chr7_2:68370673-68370692 | None:intergenic | 45.0% | |
| ! | GTTGGTTCAGTTTCAAAGGG+AGG | - | chr7_2:68370853-68370872 | Msa1071610:CDS | 45.0% |
| GGAAGACAAAGAGACTCAAG+AGG | - | chr7_2:68370900-68370919 | Msa1071610:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | - | chr7_2:68370903-68370922 | Msa1071610:CDS | 45.0% | |
| ACTAGTCCACAGGCATTAGG+TGG | - | chr7_2:68370532-68370551 | Msa1071610:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | + | chr7_2:68370541-68370560 | None:intergenic | 50.0% | |
| CCCCATATGGAATGGAGAGA+TGG | + | chr7_2:68370665-68370684 | None:intergenic | 50.0% | |
| TCTCCATTCCATATGGGGTG+TGG | - | chr7_2:68370667-68370686 | Msa1071610:CDS | 50.0% | |
| GTGCAACAAGGGGAAGGAGA+AGG | - | chr7_2:68370267-68370286 | Msa1071610:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 68370265 | 68370960 | 68370265 | ID=Msa1071610;Name=Msa1071610 |
| chr7_2 | mRNA | 68370265 | 68370960 | 68370265 | ID=Msa1071610-mRNA-1;Parent=Msa1071610;Name=Msa1071610-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|1|1|0|231 |
| chr7_2 | exon | 68370265 | 68370960 | 68370265 | ID=Msa1071610-mRNA-1:exon:14948;Parent=Msa1071610-mRNA-1 |
| chr7_2 | CDS | 68370265 | 68370960 | 68370265 | ID=Msa1071610-mRNA-1:cds;Parent=Msa1071610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
